High Speed Amino Acid Analyzer

■ Specifications ■ Setup example

System performance (by protein hydrolyzate analysis method)
Analysis time 30 min (Net) 100 –
700 mm 300 mm 700 mm
Resolution (JP) 1.2 (Thr-Ser, Gly-Ala, Ile-Leu)
Reproducibility of
RSD 0.3 % (Arg), 0.5 % (Ala)
peak retention time N2 Gas
Reproducibility of Power supply
RSD 1.0 % (Gly, His) & grounding
peak area

5m
2.5m
Detection limit 2.5 pmol (S/N=2, Asp)

Analyzer
Column Size: 4.6 mm ID × 60 mm ※ PC Installation

600 mm
Resin: Hitachi custom ion exchange resin LA8080 main unit site (Table) or
PC Rack
Pump Flow rate setting: 0.001 – 1.000 mL/min
Discharge pressure: 0 – 34 MPa
Gradient elution method with 6 solutions
Autosampler Injection method: Direct injection method
Sample vial capacity: 1,500 µL
No. of vials accommodated: 120
(with optional cooling unit installed: 100)

≥ 1,000 mm
Sample injection volume: 0.5 – 100 µL
Column oven System: Peltier
Temperature setting: 20 - 90 ˚C (in 1 ˚C steps)
Reaction unit System: Electronic heating
Temperature setting: 50 - 140 ˚C (in 1 ˚C steps)
Detector Spectrophotometer: Aberration-corrected
concave diffraction grating
Wavelength: 570 nm, 440 nm

Software Agilent OpenLAB CDS Version 2

Dimensions (excluding PC) 700 (W) × 600 (D) × 1,380 (H) mm

Weight (excluding PC) Approx. 175 kg
Temperature range 15 - 35 ˚C
Power supply 220, 240 V ± 10 V, 800 VA, 50 or 60 Hz
Nitrogen gas Nitrogen gas source must be prepared.

※ Standard column

*This logo is the trademark of Hitachi High-Technologies Corporation throughout the world.

“AminoSAAYA” is a trademark of Hitachi High-Tech Science Corporation.

CAUTION: For correct operation, follow the instruction manual when using the instrument.
Specifications in this catalog are subject to change with or without notice, as Hitachi High-Tech Science Corporation continues to develop the latest
technologies and products for its customers.
NOTICE: The system is For Research Use Only, and is not intended for any animal or human therapeutic or diagnostic use.
These data are an example of measurement; the individual values cannot be guaranteed.

www.hitachi-hightech.com/global/hhs/
Head Office
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo 105-0003, Japan

Printed in Japan (H) HSS-E013 2018.8

 Reduced workload for operators
Since the product launch, Hitachi high speed amino acid analyzers have been used by a wide range of operators, from beginners to
experts. Their various application areas include the quality control of food and pharmaceutical products as well as biochemical
research and contract analyses. This time, AminoSAAYA was developed in a compact design while maintaining the reliability and

Feel of the “Ease of use” stability cultivated over many years. The product was realized from users’ perspectives with the aims to provide a better usability for
beginner operators and a more comfortable operating environment.

With consideration for the work Space-saving compact Use of highly reliable and
and motions of operators based design (System setup area stable post-column nihydrin
on the ergonomic concept. was reduced by about 30 %). method.
Designed with consideration for the operational Compact design with the unchanged reliability and stability The analysis methods are compatible with ones of conventional
perspectives, daily work, and motions of users. of the dedicated system. models, L-8800 and L-8900.

■ Consideration for operability and safety ■ Space-saving Design ■ Reliability and stability cultivated over many years
While retaining the conventional front access design, By optimizing the locations of the flow channel system and As the analysis methods are inherited from conventional
the height of the reagent setup unit was adjusted for main unit internal module, the compact (system setup area models, the basic analytical conditions, columns (packing
better accessibility. It is devised to ensure the smooth reduced by about 30 %) system was realized. materials), and reagents that have been in use can be used
operation of daily tasks such as reagent replacement without making any changes.
and sample setup and maintenance, and the work Internal structure
performed in a half-sitting position has been minimized Reagent box
■ Post-column ninhydrin derivatization method
(The front door is also removable). Detector
By using the ninhydrin reagent which gives a characteristic
Nitrogen gas color reaction with amino acids, highly selective analysis
control unit
without the interference by contaminants (components other
than amino acids) in samples can be achieved.
Reliability and stability of Hitachi amino acid analyzer Reaction unit
55 years since the product launch. After achieving high speed, Flow line diagram
high resolution, and computerization, AminoSAAYA was born.
Column oven

Autosampler

Ninhydrin
reagent
Model L-8900
Easy operation was Pump
achieved

Features of Hitachi Amino Acid Analyzer Stock buffer

Dedicated column for ion-exchange chromatography
and various modules optimized for amino acid analysis. Select the analysis method (column, reagent, analytical
conditions) depending on the purpose. Commercially available
Model L-8800 buffer solutions and reaction reagents (dedicated reagents)
PC was installed
can be used without preparation.
in the control unit
Reaction column Unstable ninhydrin reagents usually require refrigeration.
method was employed However, with Hitachi amino acid analyzer, the two solutions
are mixed immediately before the derivatization reaction.
■ Amino acid analyzer
Therefore, there is no need for refrigeration.
Model L-8500
The system is designed specially for amino acid analysis.
First Column (3 µm particles)
Model 835 in the Nihydrin two-reagent system
Unlike general-purpose HPLC systems, it is comprised of
Column (5 µm particles) industry
Analysis time: 30 min component modules specifically designed for amino acid
Model KLA-5 Analysis time: 50 min analysis. Therefore, the system can provide excellent
Model KLA-2 Column (17 µm particles) Computerized control
performance for amino acid analysis.
Column (40 µm particles) Analysis time: 2 hrs
Analysis time: 22 hrs Installed with autosampler
 Analysis Method and Analysis Column Selection Available for various applications

Select and use the analysis column (column, analysis conditions) depending on the purpose.
Either the "Standard analysis method" or "Physiological fluid analysis method" can be selected depending
on the analyte in the sample, analysis time, resolution, etc.

たん白質加水分解物分析法
Protein （標準分析法）
hydrolyzate analysis method (standardと生体液分析法
analysis method) and physiological fluid analysis method Analysis Examples by Various Analysis Methods

■ Protein hydrolyzate analysis method (30 min) ■ High resolution standard analysis method (80 min)
Protein hydrolyzate analysis method (standard analysis method) Analysis of amino acids as
Nutrients Analyte: amino acids of protein hydrolyzate Analyte: amino acids of protein hydrolyzate
nutrients in food products
Analysis for constituent amino acids of protein
(approx. 20 components)

Response (mv)

Response (mv)
Analysis of nutritional values of
Fertilizer
amino acids contained in
Pretreatment feedstuff
(extraction, fertilizers or fodders
Protein hydrolyzation) Constituent amino Amino acid measurements
acids of protein of composition and amount
Analysis of medicinal amino acids used Retention Time (min) Retention Time (min)

Pharmaceutical for infusion or pharmaceutical products.

* STANDARD ANALYSIS COLUMN (Part No. 855-4506) * HIGH RESOLUTION PH COLUMN (Part No. 855-4508)
products Analysis of the amino acid compositions
Basic method for the simultaneous analysis of 18 Analysis method for the high resolution simultaneous analysis of 18 protein-constituting
for biopharmaceutical products, etc. protein-constituting amino acids. amino acids. It is suitable for the analysis of samples with a wide range of amino acid
contents or those containing contaminants (components other than amino acids).

Physiological fluid analysis method Analysis of amino acids for

Food
Analysis for free amino acids (approx. 40 components)
tastes and flavors in food ■ Performic acid-oxidized protein hydrolysate ■ High resolution standard analysis method (45 min)
analysis method (30 min) Analyte: amino acids of protein hydrolyzate
Analyte: amino acids of protein hydrolyzate
Analysis of amino acids in urine
Pretreatment Biological
and blood (for research purpose
(extraction, samples
only)
Foods, blood, defatting, Free amino acids Amino acid measurements

Response (mv)

Response (mv)
etc. deproteination) of amount

Analysis Column and Analysis Time Retention Time (min) Retention Time (min)

* STANDARD ANALYSIS COLUMN (Part No. 855-4506) * HIGH RESOLUTION PH TWIN COLUMN (Part No. 855-4360)
Based on the amino acids (amino acids as analytes) contained in samples, analysis time, and resolution, select the analysis
The performic acid oxidation allows the analysis of CySO3H By using the twin-column, high-resolution analysis is available
method (PH or PF) and analysis column from those listed below. (cysteic acid) and MetSON (methionine sulfone). relatively fast.

Part No. Product Name Remarks Time (min)

855-4506 STANDARD ANALYSIS COLUMN #2622, 4.6 mm × 60 mm 30 ■ Physiological fluid analysis method (110 min) ■ High-speed physiological fluid analysis
Analyte: Free amino acids method (60 min) Analyte: Free amino acids
855-4360 HIGH RESOLUTION PH TWIN COLUMN #2622, 4.6 mm × (40+40) mm 45

855-4508 HIGH RESOLUTION PH COLUMN #2622, 4.6 mm × 80 mm 80

Response (mv)

Response (mv)
Part No. Product Name Remarks Time (min)

855-4600 HIGH SPEED PF TWIN COLUMN #2623, 5.4 mm × (25+25) mm 60

855-4507 PHYSIOLOGICAL ANALYSIS COLUMN #2622, 4.6 mm × 60 mm 110 Retention Time (min) Retention Time (min)

855-4420 HIGH RESOLUTION PF TWIN COLUMN #2622, 4.6 mm × (40+40) mm 125 * PHYSIOLOGICAL ANALYSIS COLUMN (Part No. 855-4507) * HIGH SPEED PF TWIN COLUMN (Part No. 855-4600)
42 components including the protein-constituting amino acids By reducing the pressure loss of each column to about a half, the
Note) Ammonia filtering column is neccesary for the usage.
can be simultaneously analyzed. Suitable for the analysis of free twin-column enable the high-speed analysis.
* The most suited combinations for general-purpose analysis are PH method + STANDARD ANALYSIS COLUMN and PF method + amino acids (amino acids found in physiological samples).
PHYSIOLOGICAL ANALYSIS COLUMN.
 Simple operation by various special screens Operational friendliness

たん白質加水分解物分析法
Sample （標準分析法）
information entry and と生体液分析法
method selection Data Analysis screen

■ Single sample analysis ■ Sequence analysis

Sample Method Volume Sample

type name

Single sample start. Depending on the application, select a dedicated method,

then run the sequence.
Change the parameters to process the integration, then To check reproducibility, drag output data, click “Stability_
execute reanalysis. Report.rdl” in report templates.The overlay chromatogram
Data Acquisition screen and reproducibility results (retention time and area value) are
output.
Recalculation of other data is also available
during acquisition.
Report creation

Status

Acquired data Pre-created report templates are installed. Templates are

editable depending on purposes. The report result output is
also available in PDF and text formats.
By specifying the processing method in advance, reports
can be automatically created at the end of analysis.

Screen for automatic reagent amount calculation function User-friendly instrument status screen

Press the button of reagent

Maintenance information
Calculate consumption calculation in
Reagent Consumption instrument condition window,
Reaction reagent then a dialogue box appears.
The function can estimate the Mentenance Information
consumption amount of the
Status Maintenance information (parts for periodical maintenance) recorded
measurement, during editing
on the instrument can be checked.
the method, or after determining
The number of use, resetting date, and standard life are indicated.
that.
Those are indexes for maintenance to confirm the usage of parts.
With the automatic calculation
of reagent amount, the damage
to the instrument due to the Manual Mentenance button
contorol
reagent exhaustion during the
analysis or the waste overflow The operation buttons are situated to allow the manual
Buffers Waste
can be prevented. operation while checking the instrument status. The number Resetting Standard
of use date life