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BM1002

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11 views2 pages

BM1002

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wilsonaustin963
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NATIONAL INSTITUTE OF TECHNOLOGY ROURKELA

MID - SEM EXAMINATION, 2024


SESSION: 2023 – 2024 (Spring)
B.Tech., Semester- II
Subject code: BM1002 Subject Name: Introduction to Bioengineering Dept. Code: BM
No. of pages: 1 Full Marks: 30 Duration: 2 h
All parts of a question should be answered at one place.
Q. No. Particulars Marks COs*
1. How does bioengineering contribute to advancements in healthcare and 2 1
medicine?
2. A peptide with the amino acid sequence Ala-Gly-Lys-Pro-Asp-His- 2+3 2
Glu-Lys-Ala-His-Leu was dissolved in a buffer of pH 1.5. Find out:
(a) The net charge on the predominant form of the peptide.
(b) Calculate the pI of the peptide.
Amino acid pKa1 pKa2 pKa3 pI
Glycine 2.34 9.60 --- 5.97
Alanine 2.34 9.69 --- 6.00
Leucine 2.36 9.60 --- 5.98
Proline 1.99 10.60 --- 6.30
Glutamine 2.17 9.13 --- 5.65
Aspartic acid 1.88 9.60 3.65 2.77
Glutamic acid 2.19 9.67 4.25 3.22
Lysine 2.18 8.95 10.53 9.74
Histidine 1.82 9.17 6.00 7.59
3. Discuss about classification of enzymes and cite examples in each of 1.5+1.5 2
the categories. To what category do the enzymes "Glucose Oxidase,
Peroxidase and Urease" belong?
4. In an enzyme catalysed reaction, initial reaction velocity is only one 2 2
fourth of its maximum velocity. If the substrate concentration is 3 µM,
find out the value of Km.
5. What are glycosyltransferases, and how do they play a role in blood 3 2
typing?
6. What are reducing sugars? Explain with the help of examples. 2 2
7. Describe how polyunsaturated fatty acids (PUFA) function as 3 2
cardioprotective agents.
8. Represent the following fatty acid in C- and ω- system: 2 2
CH3(CH2)7CH=CH(CH2)11COOH
9. Discuss about various strategies used for immobilization of enzymes 3 3
on point of care devices.
10. Discuss the mode of functioning of a paper based diagnostic device for 5 3
blood urea nitrogen sensing.
*Course Outcomes:
At the end of the course, the students will be able to:
CO1: Gain foundational understanding of bioengineering in terms of its opportunities and challenges
CO2: Understand the role of basic biological principles for bio-based design
CO3: Integrate the fundamentals of engineering with basic biological rudiments for design based
thinking
CO4: Understand the avenues for diverse applications of bioengineering
CO5: Gain basic knowledge of artificial intelligence and its application in the field of bioengineering

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