j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4

Available online at www.sciencedirect.com

journal homepage: www.elsevier.com/locate/jmrt

Original Article

Characterization of compressed bacterial cellulose

nanopaper film after exposure to dry and humid
conditions

Hairul Abral a,*, Melati Krista Chairani a, Muhammad Dinul Rizki a,

Melbi Mahardika e, Dian Handayani b, Eni Sugiarti c,
Ahmad Novi Muslimin c, S.M. Sapuan d, R.A. Ilyas f
a
Department of Mechanical Engineering, Andalas University, 25163 Padang, Sumatera Barat, Indonesia
b
Laboratory of Sumatran Biota, Faculty of Pharmacy, Andalas University, 25163 Padang, Sumatera Barat,
Indonesia
c
Laboratory of High-Temperature Coating, Research Center for Physics Indonesian Institute of Sciences (LIPI)
Serpong, Indonesia
d
Department of Mechanical and Manufacturing Engineering, Faculty of Engineering, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia
e
Department of Biosystems Engineering, Institut Teknologi Sumatera, 35365 South Lampung, Indonesia
f
School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Skudai,
Johor Bahru, Johor, Malaysia

article info abstract

Article history: This work investigates the effects of two different levels of humidity (RH 50% and 75%) on
Received 25 September 2020 the characterizations of compressed bacterial cellulose nanopaper film. The film was
Accepted 15 January 2021 prepared with different heat treatments; compression at 25
C for 72 h and then at 100
C
Available online 27 January 2021 for 24, 72 or 120 h. Maximum tensile strength (250.7 MPa) and tensile modulus (18.6 GPa)
were measured on the film treated at 100
C for 120 h and RH 50%. However, tensile
Keywords: strength and tensile modulus dropped by 53.6% and 75.8% respectively and elongation at
Lattice strain break increased by 32% when this film was stored in more humid conditions (RH 75%). FTIR
Bacterial cellulose spectra and XRD patterns indicate changes of the deformed chain structure of the cellulose
Nanofiber as more water was incorporated between the layers of the lattice after exposure to
Relaxation humidity.
Residual stress © 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC
BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

* Corresponding author.
E-mail address: [email protected] (H. Abral).
https://doi.org/10.1016/j.jmrt.2021.01.057
2238-7854/© 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://
creativecommons.org/licenses/by-nc-nd/4.0/).
 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4 897

rinsed repeatedly with distilled water until pH 7. The wet

1. Introduction pellicle was clamped using a stainless steel flange which was
compressed using a hydraulic press at a pressure of 8 MPa for
Cellulose is the most abundant organic compound in nature 3 days at 25
C to form an unheated film (labeled as 0 h). Other
and is synthesized by a variety of microorganisms such as clamped pellicles (8 MPa) were stored in a drying oven
bacteria, algae and fungi [1]. Bacterial cellulose (BC) is secreted (Memmert UN-55) at 100
C for 24, 72 or 120 h to produce
directly as nanofibres by cellulose-producing bacteria to form heated films (labeled as 24 h, 72 h or 120 h, respectively). All
a pellicle (e.g. a thick bio-film) floating on top of a static unheated and heated films were peeled off the flange and
fermentation medium [2]. BC is a pure biopolymer, without directly stored in a desiccator chamber with relative humidity
collateral biogenic compounds like lignin, hemicelluloses, or (RH) of 50% or 75% at 25
C for 3 days. The RH level of the
pectin [3]. The BC pellicle nanofibers form a porous tridi- chamber was controlled using adsorbent sodium silicate
mensional network of cross-linked fibers immersed in a liquid (Na2SiO3) at  2% RH, and measured by a humidity meter
matrix [4]. Unlike many natural fibers, the BC can be used in (HTC-1, Hebei, China).
the production of nanopaper without the use of corrosive
chemicals [2,5]. The mechanical and electrical properties of 2.3. Characterization
the nanopaper are known to vary with water content [6].
However, stable tensile and physical properties of hydrophilic 2.3.1. Morphology investigation using field emission scanning
cellulose-based nanopaper regardless of humidity would be electron microscopy (FESEM)
an asset in many industrial applications. The FESEM samples were placed on the FESEM sample stub.
Compression is a common mechanical method in the prep- All samples were coated with carbon followed by gold for
aration of cellulose nanopaper [7e9]. Research has shown that 2 min using an argon plasma metallizer (sputter coater K575X)
compression of BC mats at 100 MPa at 70
C increased in the (Edwards Limited, Crawley, United Kingdom) to reduce the
tensile strength of about 182 MPa [9]. Cellulose film is hydrophilic electron charge. A field-emission scanning electron micro-
attracting water molecules which diffuse between through the

scope FEI Nova NanoSEM 230 (FEI, Brno-Cernovice, Czech Re-
cellulose intra- and interchains. The presence of these water public) was used with 10 kV at 20,000x magnification to
molecules may promote relaxation of the lattice strain resulting optimize observation of the surface morphology of the sample
in the cellulose chains becoming more mobile. Many previous and the fracture surface of the tensile sample was observed.
works have explored the effect of humidity on the mechanical
and physical properties of cellulose-based materials [10e13]. 2.3.2. Tensile properties
The mechanical properties of cellulose nanocrystal film have
been found to decrease with increasing humidity [10]. However, A ComeTen testing machine 95 T was used to measure tensile
the role of humidity of the lattice structure in the decrease of properties (tensile strength (TS), tensile modulus (TM), and
tensile properties remains unclear. FTIR and XRD have been elongation at break (EB)) performed with a tensile speed of
considered the effective tools to study the change of cellulose 5 mm/min at room temperature. ASTM D638-type V is the
molecular structure [14]. This paper employs these technologies standard used for tensile testing and all samples were cut to
(FTIR and XRD) to study the effects of two different levels of 63.5 mm length and 9.5 mm width [15]. Before testing, all
humidity on the characterizations of the crystalline structure of samples were conditioned for 48 h at 50 ± 5% relative humidity
compressed bacterial cellulose nanopaper film. and 25
C in a desiccator. Thickness and width of the film were
measured using a dial micrometre to 1 mm accuracy. Tensile
tests were repeated five times for each sample.
2. Materials and methods
2.3.3. FTIR
2.1. Materials Prior to FTIR characterization, dried samples were stored in a
closed chamber with RH 50% or 75% for 24 h. FTIR of the
Wet BC pellicle cuboids (250 mm  150 mm  25 mm) were sample was characterized using a PerkinElmer Frontier
purchased from the same home industry in Padang, Indonesia equipment. The film was scanned at a frequency range of
that manufactures in the form of nata de coco used in our 4000e600 cm1 at 4 cm1 resolution.
previous work [4]. The original pellicle which was purified
using distilled water and analytical grade sodium hydroxide 2.3.4. XRD testing
(Brataco Brand), and adsorbent (silica gel) was supplied by the
Andeska Laboratory and PT. Brataco, Padang, Indonesia. The crystallinity index (Icr) can be quantified using XRD
method [16]. Dried samples were stored in a closed chamber
2.2. Preparation of sample with RH 50% or 75% for 24 h before XRD characterization. X-
ray diffraction testing was carried out using PANalytical Xpert
The nata de coco pellicle was cleaned with distilled water until PRO at 25
C, 40 kV and 30 mA. The samples were scanned
pH 7 then cut into rectangles (50 mm  100 mm) with a steel from 2q ¼ 5
to 50
with l ¼ 0.154 nm. Percentage of Icr was
knife. The purified pellicle was soaked in 5% NaOH for 24 h and measured using Eq. (1) [17]:
898 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4
 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4 899

Table 1 e The crystallinity index (Icr) from Fig. 3 (without

ðI200  Iam Þ normalization), the hydrogen bond intensity (HBI) at
Icr ð%Þ ¼ x 100 (1)
I200 A3338/A1330 cm¡1 from Fig. 2, OeH stretching vibration
where I200 is the maximum intensity of the peak corre- wavenumber (cm¡1) and transmittance (T %) for samples
at RH 50% or 75% from Fig. 2.
sponding to cellulose I, and Iam is the minimum intensity of
the amorphous fraction between the 101 and 200 peak. Sample RH (%) Icr (%) HBI OH-Stretching
3340 cm1 T%
2.3.5. Statistical analysis 0h 50 89.34 1.9 3337 18.46
24 h 88.63 2.0 3339 17.83
Experimental data were analyzed using IBM SPSS Statistics 72 h 87.66 2.0 3338 28.12
25.0 (IBM Corporation, Chicago, USA). One-way analysis of 120 h 91.45 2.1 3340 26.12
variance (ANOVA) and p-test were used to identify the sig- 0h 75 85.44 2.1 3339 23.19
24 h 90.93 2.1 3340 19.51
nificance of any effects of dry and humid conditions (RH 50%
72 h 91.12 2.2 3342 23.51
and 75%) on tensile properties of the nanopaper film. 120 h 91.26 2.0 3345 25.87

3. Results and discussion e), OeH bending (Fig. 2f-g), CeC and CeO skeletal vibrations
(Fig. 2h-i), and CeO stretching (Fig. 2j-k) respectively [19e22].
3.1. FESEM morphology The wavenumbers of OeH stretching shifted to a higher value
(see Table 1) as humidity increased. This is attributable to the
Fig. 1 shows the FESEM morphology of the BC nanopaper film hydrogen bond interaction between the OeH groups of cellu-
for each treatment. The appearance of the film surfaces at RH lose and water [23]. The shape of the band at RH 50% was
50% (Fig. 1a, c) or 75% (Fig. 1b, d) was similar; humidity did not broader as a result of the more disoriented crystal (Fig. 2c).
obviously change the appearance of the films. Longer This shape became sharper at higher humidity (RH 75%). A
compression duration at 100
C increased in compactness of similar phenomenon is also observed for other functional
the nanofiber (marked with a white arrow in Fig. 1e, f). A groups (Fig. 2d-i). The increased sharpness of the band for the
similar phenomenon was also observed on the fracture sur- hydroxyl groups was attributed to an increase in intra- and
face of the compressed film which was heated at 100
C for intermolecular hydrogen bonds and chain reordering [23,24].
120 h and shows more fractions of high nanofiber density This explanation is supported by the ratio value between the
(marked with a white arrow in Fig. 1h) in comparison to the absorbance bands at 3324/1320 cm1 (see Table 1) demon-
compressed film treated at 25
C for 120 h (Fig. 1g). This denser strating the increased hydrogen bonding [25]. A similar trend
nanofiber section has greater interconnection between the was also presented by the wavenumber of about 1640 cm1
cellulose molecules on the surface through hydrogen bonding. (Fig. 2f-g) showing lower value at lower humidity. This phe-
The highly interconnected nanofibers have low mobility nomenon is similar to previous work that the absorption
which can obstruct the movement of mobile nanofibers. maximum of the water peak shifts slightly toward a higher
Consequently, more fractions of the low nanofiber mobility wavenumber as the relative humidity increases [23]. The peak
may increase tensile strength and modulus, and decrease intensity at 1640 cm1 for BC sample exposed to RH 50%
elongation at break of the BC film (see Fig. 4). In contrast to decreased as heating duration increased (Fig. 2f) due to water
Fig. 1h, g shows a fracture surface with a larger number of big evaporation [26]. In contrast, the intensity at this band
cavities, and less compact network structure. Therefore, the (1640 cm1) for BC exposed to RH 75% shows a different trend
cellulose chain as shown in Fig. 1g was more mobile than that (Fig. 2g). The 24 h and 72 h samples had a stronger peak in-
in Fig. 1h. Consequently, BC nanopaper with the mobile tensity than the 0 h sample. The hydroxyl groups in the cel-
nanofiber had low tensile strength and modulus but its elon- lulose polymer can reform hydrogen bonds between different
gation at break was high. cellulose polymers or within the polymer itself [27]. The
intramolecular bonds give stiffness to the polymer chain,
3.2. FTIR spectra while the intermolecular bonds allow the linear polymers to
reform sheet structures [28]. Intermolecular bond formation
The effect of treatment on the functional groups of the cel- leads to an increase in the rigidity of the cellulose chain
lulose can be observed using FTIR spectroscopy [18]. Fig. 2a network resulting from an increase in the structural relaxa-
and b displays FTIR for each sample averaged from triplicate tion. However, after longer heating durations the peak in-
measurements. All spectra display a similar pattern, however, tensity became weaker indicating a decrease in hydrophilic
there are differences in the broadness, intensity and wave- nature and probably an increase in the amount of the aggre-
number value of the peak (see Fig. 2c-k for more details). The gated crystalline cellulose microfibrils which are less hydro-
main peaks at about 3310, 2897, 1640, 1328 and 1030 cm1 philic than nonaggregated microfibrils.
correspond to OeH stretching (Fig. 2c), CeH stretching (Fig. 2d-

Fig. 1 e FESEM morphology of nanopaper film prepared with various treatments. Surface of film treated at 25
C for 72 h and
RH 50% (a) or RH 75% (b); Surface of film treated at 100
C for 24 h at RH 50% (c) or RH 75% (d); for 72 h (e) or 120 h (f) at RH 50%;
from fracture surface at 25
C for 72 h and RH 50% (g) or at 100
C for 120 h at RH 75% (h).
900 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4

Fig. 2 e FTIR spectra of BC nanopaper films formed with different heating durations at 100
C (aeb) The full spectrum from
4000 to 250 cm¡1 (cek) sections of the spectrum expanded to show changes in peaks related to specific functional groups
after storage in a closed chamber with RH 50% or RH 75%.

The peaks at wavenumber 1290-1390 cm1 at RH 50% phenomenon results in an increase in the molecular vibration
(Fig. 2h) were broad probably corresponding to a highly dis- recorded in FTIR spectra as a shift to a higher wavenumber
oriented crystal structure [24]. After RH 75% exposure (Fig. 2i) value. These results reconfirm that the cellulose film with
the peak became sharper which can be related to the reduc- higher deformation of cellulose structures was more relaxed
tion of lattice strain [29e31]. The band at about 1030 cm1 and had a higher sensitivity to higher humidity.
corresponding to CeO stretching is related to the backbone
structure of cellulose [32]. The 0 h films for both RH 50% and 3.3. XRD pattern
75% showed peaks at the lowest wavenumber at 1029 cm1
and 1031 cm1 respectively. These bands at RH 75% (Fig. 2k) Fig. 3 displays XRD patterns of samples exposed to 50% or 75%
were sharper than that at RH 50% (Fig. 2j). Wavenumber po- RH. The main peaks were observed at about 2q ¼ 14.5
and
sition at RH 75% shifted from 1031 cm1 (0 h film) to 1035 cm1 2q ¼ 22.5
corresponding to the (101) and (200) crystal plane
(120 h film). This shifting was more visible than in the RH 50% which indicates a semi-crystalline nature [35]. This is char-
film. The reason for the 0 h films has the lowest wavenumber acteristic of pure bacterial cellulose [4]. Compression results in
and the broadest band is because the cellulose structure was a decrease in the peak intensity and a broadening of the peaks
probably experiencing the strongest deformation [33,34]. This as shown by Fig. 3a and b without normalization (i). Table 1
deformation causes an increase in the microfibril density, a displays the crystallinity index (Icr) of each sample as a func-
decrease in the bond lengths and the interlayer spacing of tion of heating duration for various RH. Icr value overall in-
chains, consequently a decrease in the vibration of the creases as temperature increases. The X-ray diffraction
deformed molecules. These cellulose networks in higher hu- profile, notably at RH 75%, presents narrower and sharper
midity interact with more water molecules creating intra- and peaks as a result of an increase in the crystallinity of the
intermolecular hydrogen bonds [27]. Consequently, the cel- structures (see inset of Fig. 3b). This suggests that absorption
lulose structures expand and molecular chains move into of water molecules into the structure did not decrease in the
more thermodynamically favourable positions. This Icr value but did increase the crystal spacing. When the
 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4 901

Fig. 3 e XRD curves of nanopaper with RH 50% (a) and 75% (b) without (i) and with normalization (ii).

material is under stress, the interlayer spacing of cellulose structures and releasing activation energy [38]. A slight in-
chains can increase or decrease, which leads to a shift in the crease in the bound water was measured on the ratio between
peak positions in an XRD pattern [36]. the absorbance bands at 3400 and 1320 cm1 at RH 75%
According to previous research, a shift the peak position to compared to RH 50% [39].
the right side after compression (see normalized XRD curve of
Fig. 3a(ii)) can be associated with an increase in compressive 3.4. Tensile properties
residual stress resulting from a decrease in the interlayer
spacing of the cellulose polymer chains [36e38]. The peak Fig. 4 shows TS (a), TM (b), and EB (c) of cellulose film after
position for the unheated nanopaper film (0 h film) at RH 50% exposure to RH 50% or 75%. After further heating, TS and TM
was 2q ¼ 22.7
lower than the same film after exposure to 75% increased and EB decreased. An increase in these properties
RH (2q ¼ 22.9
) probably due to different lattice spacing [38]. was obviously in the RH 50% attributed to decreased degree of
This peak (200 plane) position for the 120 min heated nano- molecular chain mobility resulting from the denser chain
paper after storing in RH 50% was still at a larger angle than network, greater amount of the interconnected nanofibers,
that of the unheated film. This is because the increase in and the presence of the compressive residual stress. The
absorbed water molecules at this degree of humidity was not external tensile stress must overcome the compressive re-
sufficient to restructure the lattice plane. In contrast, RH 75% sidual stress before the crack tips experiences sufficient ten-
exposure caused the peak to shift toward the left side or to sile stress to propagate. Maximum TS (250.7 MPa) and TM
smaller angles for all samples (see normalized XRD curve of (18.6 GPa) were measured in the film treated at 100
C for 72 h
Fig. 3b(ii)) showing an expansion of lattice planes as a result of after RH 50% exposure (246% and 597% increases compared to
the presence of more water molecules relaxing the chain the nontreated film). In this case, TS improved by 116%
902 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4

Fig. 4 e TS (a), TM (b) and EB (c) for unheated and heated samples.

compared to the same film exposed to RH 75%. However, the residual stress. This internal stress opposes external tensile
value of TS dropped from 250.7 MPa to 116.2 MPa (a decrease stress increasing the TS and TM. However, exposure to higher
by 53.6%) at this humidity and surprisingly EB increased after humidity (RH 75%) resulted in a relaxation of the lattice
the film was stored in a more humid room. This probably planes, thus reducing the compressive residual stress.
corresponds to a decrease in compressive residual stress Consequently, TS and TM dropped and the stiffness of the film
resulting from relaxation of the cellulose chain structure decreased.
which was promoted by more water molecules. This result is
in agreement with Fig. 3b (XRD pattern) showing more relax-
ation for samples stored in higher humidity (RH 75%). The Declaration of Competing Interest
incorporation of more water molecules at RH 75% restructured
chain networks via inter- and intramolecular hydrogen bonds The authors declare no conflict of interest.
leading to an increase in the interlayer spacing and the length
of the bonds, thus an increase in the cellulose chain mobility.
Significantly more mobile chain structures were observed in
Acknowledgement
unheated (0 h) film which had a higher elongation at break at
RH 75% (EB ¼ 36%) than 50% (EB ¼ 25%).
Acknowledgement is addressed to Universitas Andalas for
supporting research funding with project name Riset Publikasi
Guru Besar, number T/5/UN.16.17/PP.IS-KRP1GB/LPPM/2020.
4. Conclusion

Tensile properties of compressed BC film were found to be references

strongly dependent on the humidity level and the presence of
the residual stress. Longer heating duration increased tensile
strength and the tensile modulus of the film particularly when [1] Satari B, Karimi K, Kumar R. In: Cellulose solvent-based
under conditions of lower humidity (RH 50%). This increase pretreatment for enhanced second-generation biofuel
corresponds to lower cellulose chain mobility due to a denser production: a review, vol. 3. Royal Society of Chemistry; 2019.
https://doi.org/10.1039/c8se00287h.
network structure of the nanofibril and higher compressive
 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4 903

[2] Hervy M, Blaker JJ, Braz AL, Lee KY. Mechanical response of
M, Marinc
[18] Kolar J, Strlic
ek M. IR pulsed laser light interaction
multi-layer bacterial cellulose nanopaper reinforced with soiled cellulose and paper. Appl Phys Mater Sci Process
polylactide laminated composites. Compos Part A Appl Sci 2002;75:673e6. https://doi.org/10.1007/s003390201309.
Manuf 2018;107:155e63. https://doi.org/10.1016/ [19] Syafri E, Kasim A, Abral H, Sudirman, Sulungbudi GT,
j.compositesa.2017.12.025. Sanjay MR, et al. Synthesis and characterization of cellulose
[3] Tischer PCSF, Sierakowski MR, Westfahl H, Tischer CA. nanofibers (CNF) ramie reinforced cassava starch hybrid
Nanostructural reorganization of bacterial cellulose by composites. Int J Biol Macromol 2018;120:578e86. https://
ultrasonic treatment. Biomacromolecules 2010;11:1217e24. doi.org/10.1016/j.ijbiomac.2018.08.134.
[4] Abral H, Lawrensius V, Handayani D, Sugiarti E. Preparation [20] Nacos MK, Katapodis P, Pappas C, Daferera D, Tarantilis PA,
of nano-sized particles from bacterial cellulose using Christakopoulos P, et al. Kenaf xylan - a source of biologically
ultrasonication and their characterization. Carbohydr Polym active acidic oligosaccharides. Carbohydr Polym
2018;191:161e7. 2006;66:126e34. https://doi.org/10.1016/j.carbpol.2006.02.032.
[5] Abral H, Hartono A, Hafizulhaq F, Handayani D, Sugiarti E, [21] Sun XF, Xu F, Sun RC, Fowler P, Baird MS. Characteristics of
Pradipta O. Characterization of PVA/cassava starch degraded cellulose obtained from steam-exploded wheat
biocomposites fabricated with and without sonication using straw. Carbohydr Res 2005;340:97e106. https://doi.org/
bacterial cellulose fiber loadings. Carbohydr Polym 10.1016/j.carres.2004.10.022.
2018;206:593e601. https://doi.org/10.1016/ [22] Abral H, Ariksa J, Mahardika M, Handayani D, Aminah I,
j.carbpol.2018.11.054. Sandrawati N, et al. Transparent and antimicrobial cellulose
[6] Rebelo A R, Archer AJ, Chen X, Liu C, Yang G, Liu Y. film from ginger nanofiber. Food Hydrocoll 2020;98:105266.
Dehydration of bacterial cellulose and the water content https://doi.org/10.1016/j.foodhyd.2019.105266.
effects on its viscoelastic and electrochemical properties. Sci [23] Velazquez G, Herrera-Go  mez A, Martı́n-Polo MO.
Technol Adv Mater 2018;19:203e11. https://doi.org/10.1080/ Identification of bound water through infrared spectroscopy
14686996.2018.1430981. in methylcellulose. J Food Eng 2003;59:79e84. https://doi.org/
[7] Henriksson M, Berglund LA, Isaksson P, Lindstro € m T, 10.1016/S0260-8774(02)00428-4.
Nishino T. Cellulose nanopaper structures of high toughness. [24] Abral H, Ariksa J, Mahardika M, Handayani D, Aminah I,
Biomacromolecules 2008;9:1579e85. https://doi.org/10.1021/ Sandrawati N, et al. Effect of heat treatment on thermal
bm800038n. resistance, transparency and antimicrobial activity of
[8] Hu L, Zheng G, Yao J, Liu N, Weil B, Eskilsson M, et al. sonicated ginger cellulose film. Carbohydr Polym
Transparent and conductive paper from nanocellulose fibers. 2020;240:116287. https://doi.org/10.1016/
Energy Environ Sci 2013;6:513e8. https://doi.org/10.1039/ j.carbpol.2020.116287.
c2ee23635d. [25] Oh SY, Dong IY, Shin Y, Hwan CK, Hak YK, Yong SC, et al.
[9] Retegi A, Gabilondo N, Pen ~ a C, Zuluaga R, Castro C, Gan
~ an P, Crystalline structure analysis of cellulose treated with
et al. Bacterial cellulose films with controlled sodium hydroxide and carbon dioxide by means of X-ray
microstructure-mechanical property relationships. Cellulose diffraction and FTIR spectroscopy. Carbohydr Res
2010;17:661e9. https://doi.org/10.1007/s10570-009-9389-7. 2005;340:2376e91. https://doi.org/10.1016/
[10] Wu Q, Meng Y, Concha K, Wang S, Li Y, Ma L, et al. Influence j.carres.2005.08.007.
of temperature and humidity on nano-mechanical [26] Cheng D, Li T, Smith G, Yang J, Hang C, Miao Z, et al.
properties of cellulose nanocrystal films made from Influence of calcium chloride impregnation on the thermal
switchgrass and cotton. Ind Crop Prod 2013;48:28e35. https:// and high-temperature carbonization properties of bamboo
doi.org/10.1016/j.indcrop.2013.03.032. fiber. PLoS One 2019;14:1e12. https://doi.org/10.1371/
[11] Gennadios A, Park HJ, Weller CL. Relative humidity and journal.pone.0212886.
temperature effects on tensile strength of edible protein and [27] Chami Khazraji A, Robert S. Self-assembly and
cellulose ether films. Trans ASAE 1993;36:1867e72. https:// intermolecular forces when cellulose and water interact
doi.org/10.13031/2013.28535. using molecular modeling. J Nanomater 2013;2013:1e12.
[12] Yousefi H, Faezipour M, Hedjazi S, Mousavi MM, Azusa Y, https://doi.org/10.1155/2013/745979.
Heidari AH. Comparative study of paper and nanopaper [28] Bo€ rjesson M, Westman G. Crystalline nanocellulose d
properties prepared from bacterial cellulose nanofibers and preparation, modification, and properties. Intech; 2016. p. 13.
fibers/ground cellulose nanofibers of canola straw. Ind Crop https://doi.org/10.5772/57353.
Prod 2013;43:732e7. https://doi.org/10.1016/ [29] Chalmers JM, Mackenzie MW, Willis HA, Edwards HGM,
j.indcrop.2012.08.030. Lees JS, Long DA. FTIR spectroscopic studies of isotactic
[13] Ebrahimzadeh PR, Kuba  t DG. Effects of humidity changes on polypropylene films under stress. Spectrochim Acta Part A
damping and stress relaxation in wood. J Mater Sci Mol Spectrosc 1991;47:1677e83. https://doi.org/10.1016/0584-
1993;28:5668e74. https://doi.org/10.1007/BF00367845. 8539(91)80005-4.
[14] Dai D, Fan M. Investigation of the dislocation of natural fibres [30] Svenson MN, Thirion LM, Youngman RE, Mauro JC,
by Fourier-transform infrared spectroscopy. Vib Spectrosc Bauchy M, Rzoska SJ, et al. Effects of thermal and pressure
2011;55:300e6. https://doi.org/10.1016/j.vibspec.2010.12.009. histories on the chemical strengthening of sodium
[15] ASTM D882 - 12. Standard test method for tensile properties aluminosilicate glass. Front Mater 2016;3:1e11. https://
of thin plastic sheeting. Am Soc Test Mater 2002. doi.org/10.3389/fmats.2016.00014.
[16] Ju X, Bowden M, Brown EE, Zhang X. An improved X-ray [31] Brown EN, Clausen B, Brown DW. In situ measurement of
diffraction method for cellulose crystallinity measurement. crystalline lattice strains in phase IV
Carbohydr Polym 2015;123:476e81. https://doi.org/10.1016/ polytetrafluoroethylene. J Neutron Res 2007;15:131e8.
j.carbpol.2014.12.071. https://doi.org/10.1080/10238160701372620.
[17] Segal L, Creely JJ, Martin AE, Conrad M. Empirical method for [32] Wiley JH, Atalla RH. Band assignments in the Raman spectra
estimating the degree of crystallinity of native cellulose of celluloses. Carbohydr Res 1987;160:113e29. https://doi.org/
using the X-ray diffractometer. Textil Res J 1958:786e94. 10.1016/0008-6215(87)80306-3.
904 j o u r n a l o f m a t e r i a l s r e s e a r c h a n d t e c h n o l o g y 2 0 2 1 ; 1 1 : 8 9 6 e9 0 4

[33] Tanpichai S, Quero F, Nogi M, Yano H, Young RJ, Lindstro€ m T, [36] Epp J. X-ray diffraction (XRD) techniques for materials
et al. Effective young’s modulus of bacterial and characterization. Elsevier Ltd; 2016. https://doi.org/10.1016/
microfibrillated cellulose fibrils in fibrous networks. B978-0-08-100040-3.00004-3.
Biomacromolecules 2012;13:1340e9. https://doi.org/10.1021/ [37] Bandyopadhyay R, Selbo J, Amidon GE, Hawley M.
bm300042t. Application of powder X-ray diffraction in studying the
[34] Kac
ura kova
 M, Smith AC, Gidley MJ, Wilson RH. Molecular compaction behavior of bulk pharmaceutical powders. J
interactions in bacterial cellulose composites studied by 1D Pharm Sci 2005;94:2520e30. https://doi.org/10.1002/jps.20415.
FT-IR and dynamic 2D FT-IR spectroscopy. Carbohydr Res [38] Cao W, Ma C, Tan S, Ma M, Wan P, Chen F. Ultrathin and
2002;337:1145e53. https://doi.org/10.1016/S0008-6215(02) flexible CNTs/MXene/cellulose nanofibrils composite paper
00102-7. for electromagnetic interference shielding. Nano-Micro Lett
[35] Tsouko E, Kourmentza C, Ladakis D, Kopsahelis N, Mandala I, 2019;11:1e17. https://doi.org/10.1007/s40820-019-0304-y.
Papanikolaou S, et al. Bacterial cellulose production from [39] Poletto M, Ornaghi Júnior HL, Zattera AJ. Native cellulose:
industrial waste and by-product streams. Int J Mol Sci structure, characterization and thermal properties. Materials
2015;16:14832e49. https://doi.org/10.3390/ijms160714832. (Basel) 2014;7:6105e19. https://doi.org/10.3390/ma7096105.