2021 Lab Manual For CXC
2021 Lab Manual For CXC
2021 Lab Manual For CXC
List of labs
YEAR I
1 Diffusion To demonstrate diffusion in a liquid ORR
15. Metals and non – Metals To determine the reactivity of metals with *MM &
metals &Non-metals dilute H2SO4 *ORR
16. Excretion To draw the internal structure of a D
mammalian kidney
17. Health and sanitation *PD
Planning and Designing
Problem Statement: A problem statement is a brief description of the issue(s) that need to be addressed.
Hypothesis: Your hypothesis is like a good guess or prediction of what you expect to find from carrying out
your experiment. Even though your hypothesis is a good guess, it is stated as a definite
prediction e.g. X 'will' or 'will not' happen. In addition, a well-developed hypothesis should
begin with a short phrase which links back to the theoretical basis for the experiment.
Aim: An aim identifies the purpose of the investigation. It is a straightforward expression of what the
researcher is trying to find out from conducting an investigation. The aim typically involves the
word “investigate” or “investigation”.
Apparatus & Materials: A listing of all equipment, and chemicals needed to carry out the experiment
Method: This section of the report gives a detailed account of the procedure that will be followed in
completing the experiment discussed in the report. This is very important as it gives the reader a
clear understanding of the experiment, and also serves as a set of instructions for anyone desiring
to replicate the study in the future. For a planning and design lab the method is written in
PRESENT TENSE.
Variables
The things that are changing in an experiment are called variables. A variable is any factor, trait, or condition
that can exist in differing amounts or types. An experiment usually has three kinds of variables: controlled,
manipulating and responding.
● Controlled – A control variable is the one element that is not changed throughout an experiment, because
its unchanging state allows the relationship between the other variables being tested to be better understood.
● Responding – The responding (or Dependent) Variable is the variable that changes as a result of the
changes in the manipulated variable.
Expected results – This must be clearly stated. This can be in the form of a table or graph. Often times it is a
short paragraph describing what you expect to happen.
Discussion of expected results / Treatment of results – Links the expected results to the hypothesis
Precautions / limitations – Any shortcomings of the design which may become sources of error.
Experiment: 1
Date:
Topic: Diffusion
Diagram:
Method:
Observation:
1. Define diffusion
2. Discuss results
3. Examples of diffusion in living organisms.
Conclusion: From experiment answer the Aim.
Criteria:
__________
10
Experiment # 2
Date:
Topic: Osmosis
Aim: To investigate the effects of osmosis on potato cells in salt solution of varied concentration.
Apparatus: measuring cylinder, ruler, scalpel, three petri dishes, spoon, stopwatch
Materials: label tape, salt solutions 5% and 10 %, pure water, blotting paper
Diagram:
Method:
1. Cut six strips of potato into exact lengths of 3cm.
2. Label three petri dishes A, B and C respectively.
3. In dish A add 20 cm3 of pure water.
4. In dish B add 20 cm3 of 5% salt solution.
5. In dish C add 20 cm3 of 20% salt solution.
6. Add TWO strips each to each petri dish and start timer for 15minutes.
7. After 15 minutes, remove strips and place on blotting paper.
8. Measure and record the new lengths of each strip in a results table.
Observation / result:
TABLE SHOWING THE EFFECT OF OSMOSIS ON POTATO CELLS PLACED IN WATER AND SALT SOLUTIONS
1 2 1 2
A (control)
B ( 5% salt solution)
1. Define osmosis
2. Explain the trend in the results (how did the length of strip change with the amount of water particles in
each petri dish A, B and C).
3. Why did the strips look and feel that way,
4. How did osmosis occur in each petri dish (direction of the diffusion gradient)?
5. What effect does osmosis have on the potato cells? (shrinking potato cells (flaccidity) and damaging cell
parts or swelling cells (turgidity) causing bulging as the amount of water in cell sap increases.
6. Explain what layer of the potato cell was partially permeable.
__________
10
Experiment: # 3
Date:
Topic:
Hypothesis:
Aim:
Apparatus & Materials:
Diagram (if applicable)
Method
Variables
● Controlled
● Manipulated
● Responding
Expected results
Discussion of expected results
Precautions / limitations
Hypothesis
✔ Stated clearly 1
✔ Testable 1
Method
✔ Suitable 1
✔ Manipulated or responding variable included 1
Expected result
✔ Reasonable 1
✔ Linked with method 1
___________
10
Experiment # 4
Date:
Topic:
Apparatus/ materials:clamp and stand, hot plate, three 200 ml beakers, water, aluminium foil, thermometers,
cotton wool, stop watch,
Method:
1. Label three beakers A, B, and C.
2. Wrap beaker A with aluminium foil, beaker B with cotton wool and leave beaker C unwrapped.
3. Boil 500ml of water.
4. Using a measure cylinder measure and pour 150 ml of boiled water into each of the labelled beakers.
5. Using a thermometer measure the temperature of the water in each beaker at one minute intervals for ten
(10) minutes.
6. Record the results in a table and plot a line graph to show time interval against temperature.
Results: (Write suitable title for table with correct headings table)
b. Evaluation of data 2
c. Relationship between temperature and time
d. Appropriate conclusion. 1
e. Source of error/ Precaution/ Limitation 1
f. Reflection 1
___________
10
Date:
Topic: Electricity
Skill Assessed: ORR
Apparatus: Battery, connecting wires, switch, copper, bulb (lamp), crocodile clips, plastic, paper,
paper clip, glass, rubber, leather
1. Join cell, bulb, switch together with connecting wire as shown in diagram above.
2. Use crocodile clips to connect different material such as plastic, paper clip, copper etc in the gap to
complete the circuit.
3. Record observation of what happens as the materials are placed in the circuit when switch is opened and
closed.
4. Record results in a suitable table
Criteria Marks
Grammar 1
Format 1
10
Experiment 6
Date:
Topic: Acid, Base and Mixture
Apparatus / materials: test tube, test tube racks, red and blue litmus paper, bleach, soft drink, soap powder
solution ( other household chemicals) universal indicator solution
Procedure:
Observation:
Title: _____________________________________________________________________________________
Household Red litmus Blue litmus Colour change with universal pH value Inference
chemicals indicator solution
From To
Skill assessed: ORR
Criteria
Organization
Table
⮚ 7 – 10 correct 2
⮚ 3 – 6 correct 1
⮚ Below 3 0
⮚ 7 – 10 correct 2
⮚ 3 – 6 correct 1
⮚ Below 3 0
___________________
10
Experiment 7
Date:
Topic:
Problem Statement: when the bacteria in your mouth feed on food remains between your teeth, they produce
acid. This acid will destroy or eat away the enamel of your teeth, which will eventually
lead to tooth decay. You are given three brands of toothpaste; colgate fluoride, colgate
with hydrogen peroxide an colgate with baking soda. Plan and design an experiment to
determine which brand of toothpaste would be more effective in preventing tooth decay.
__________________________________________________________________________________________
Criteria
Hypothesis
✔ Stated clearly 1
✔ Testable 1
Method
✔ Suitable 1
✔ Manipulated or responding variable included 1
Expected result
✔ Reasonable 1
✔ Linked with method 1
___________
10
Experiment # 8
Date:
Topic: photosynthesis
Drawing
Points
Clarity
Accuracy
Labelling
Title
- View/orientation stated 1
- Written in capitals, underlined and placed below the drawing 1
Magnification
Total 10 marks
Experiment # 9
Date:
Topic: Photosynthesis
Apparatus: Heat source, wire gauze, large beaker, boiling tube, dropper, test tube holder, tongs, tile / plate
Method
1. Set up heat source with wire gauze before placing beaker half – filled with water (water bath) on it.
2. Place the leaf in boiling water for three (3) minutes to soften it.
3. Remove leaf from water and place in a boiling tube with 20ml of alcohol (enough to cover the leaf) for
15 minutes or until leaf is decolorized.
4. Carefully top up alcohol in tube in water bath, if necessary.
5. Careful remove decolorized leaf and gently wash with water.
6. Spread the leaf on a flat surface and add 3 – 5 drops of iodine solution on the decolorized leaf.
7. Note observation.
Observation:
Note the following:
● Texture of leaf before and after being boiled.
● Colour of alcohol before leaf was placed in it and colour seen after the 15 minutes
● Colour of leaf after being removed from alcohol
● Colour seen upon addition of iodine to leaf.
1. What is photosynthesis?
2. State the reasons why it was necessary to
i. Boil the leaf in water
ii. Boil leaf in alcohol
iii. Wash the decolourised leaf
3. State why testing the leaf for starch is a reliable indication that photosynthesis has taken place.
4. State two importance of photosynthesis
____________
10
Experiment # 10
Date:
Topic: Nutrition
Skill Assessed: PD
Problem statement: Jimmy, Carla and Kacy were engaged in a heated discussion at breakfast over which bread
contains the most nutrients. Jimmy thinks its white bread; Carla thinks its multigrain bread while Kacy thinks its
wheat bread. Plan and design an experiment to find out which bread contains the most nutrients.
Criteria
Hypothesis
✔ Stated clearly 1
✔ Testable 1
Method
✔ Suitable 1
✔ Manipulated or responding variable included 1
Expected result
✔ Reasonable 1
✔ Linked with method 1
___________
10
Experiment # 11
Date:
Topic: Reproduction
Aim: To draw, label and annotate the whole and half red pea
Drawing
Points
Clarity
Accuracy
Labelling
- View/orientation stated 1
- Written in capitals, underlined and placed below the drawing 1
Magnification
𝑠𝑖𝑧𝑒 𝑜𝑓 𝑑𝑟𝑎𝑤𝑖𝑛𝑔
Magnification = 𝑠𝑖𝑧𝑒 𝑜𝑓 𝑠𝑝𝑒𝑐𝑖𝑚𝑒𝑛
Total 10 marks
Experiment 12
Date:
Topic: Respiration
Method:
1. Draw a table to record the results to show the pulse rate at rest, jogging and running.
2. Place students in groups of two (2).
3. Allow PersonA to sit quietly for one (1) minutes to be completely relaxed.
4. Count the number of pulses for one (1) minute and record in the table.
5. Allow person to jog for two (2) minutes.
6. Immediately after,count the number of pulse for one minute and record.
7. Allow Person A to rest for one (1) minute.
8. Immediately after allow person A to run for two (2) minutes and afterwards count the number of
pulses for one 1 minute and record in the table.
9. At the end of this allow person A to rest for one (1) minute.
10. Take the pulse for one (1) minute and record the result as recovery one (R1).
11. Allow the person to rest again for one (1) minute.
12. Count the pulse for one (1) minute afterwards and record this result as the 2nd recovery (R2).
13. Repeat steps 11 and 12, but record the result as 3rd recovery (R3).
14. Repeat steps 2 -13 with person B of the group.
15. Plot a line graph using data present in the table for comparison of pulse rate at rest, jogging and
running for persons A and B.
Results: (Title for results table)
Conclusion: How did the pulse rate change with the activities and why?
Skill Assessed: Observation, Recording and Reporting & Analysis and Interpretation
c. include equations; 1
2. Evaluating details
a. Explain the increase pulse beat means increase in oxygen intake and
carbon dioxide removal for respiration 2
b. Deduce that lactic acid is formed in anaerobic respiration 1
c. Deduce that panting means oxygen debt is being repaid 1
d. Deduce from data that the healthier individual has lower pulse rates overall _________
10
Experiment: # 13
Date:
Aim: To determine blood matching by simulating antigen-antibody reactions using food colouring.
Apparatus / Materials: test tube rack with test tubes, spotting tile, measuring cylinder (0-10 ml),
droppers, 4 pieces of straw / glass rod, food colouring- red and blue, water,
Method:
1. Label recipient’s blood type in tile and donor’s blood type in test tube -A. B, AB & O.
2. Measure and collect from ‘blood bank stock’ 5cm3 of each donor’s blood type A. B. AB & O.
● Red colouring- represents blood group A
● Blue colouring – represents blood group B
● Purple colouring ( red & blue mix)– represents blood group AB
● Pure water – represents blood group O
3. Add two drops each in four spots on the tile for each recipient’s blood type A, B, AB and O from the
teacher’s stock.
4. Use straw/splint to add two drops each of donor’s blood group A to recipient’s blood group A. Stir and
note colours in a result table.
5. Rinse straw/glass rod thoroughly and repeat step 4 with recipient’s blood group B, then to recipient’s
blood group AB and, then to recipient’s blood group O. If there is a colour change, then clumping
(agglutination) has occurred.
6. Repeat steps 4 – 5 using donor’s blood group B with a different straw/glass rod, then use donor’s blood
group AB and finally donor’s blood group O with recipients’ blood.
DON
OR’S RECEIPIENT’S BLOOD
BLO A B AB O
OD
A
AB
4. Which blood type can donate blood to any other blood types? Explain your answer.
5. Which blood can receive blood from any other blood types? Explain your answer.
CRITERIA
Use of background knowledge (1)
Experiment: # 14
Date:
IMPLEMENTATION
Skill assessed: AI
Method 1
(Change of tense to past tense)
Results 4
● Accurate and appropriately presented observation
● Tabular form with title
● Experimental setup along with results
Discussion 5
● Define nutrition, nutrients and the importance of eating healthy
● Trends on results identified (which bread has more nutrient)
● Explain trends (use of scientific evidence)
● Include other info to compare nutrients if test results were the same
Limitation 1
Precaution 1
Source of error 1
Reflection 5
Conclusion 2
● Stated
● Related to aim
__________________
20
Experiment: # 15
Date:
Skill Assessed: Measurement and Manipulation and Observation, Recording and Reporting
Aim: To determine the reactivity of metals with dilute Hydrochloric Acid (HCl)
Apparatus/ materials:test tubes, test tube rack, aluminium, magnesium ribbon, copper, iron filings, zinc powder
hydrochloric acid, sulphuric acid, measuring cylinder, splint, matches,
Method:
1. Weigh 0.5 grams of each metal and place it into a labelled test tube.
2. Using a measuring cylinder measure out 5cm3 of hydrochloric acid and pour it into the test tube labelled
zinc.
3. Observe for effervescence noting how vigorous the reaction is.
4. Place a lighted splint at the end of the test tube and note what occurs.
5. Repeat steps 2 – 4 using the other metals.
6. Record the results in a table.
Magnesium turnings
Aluminium powder
Iron powder
Copper powder
In the results table for each observation state whether the reaction was very vigorous, vigorous, moderately vigorous or
no vigour and state whether the metal has diminished or if effervescence has occurred. State if a popping sound was heard
with the reaction of the glowing splint. Under the heading inference state whether hydrogen gas was given off and
include the equation that governs the reaction.
Conclusion: How do acids react with metals in terms of where they are found in the reactivity series?
Skill Assessed: Manipulation / Measurement
Criteria
10
Skill assessed : ORR
Table
- Title stated in heading of each column 1
- Table title stated accurately in ALL capital letters 1
Observations
- All 5 correct 2
- 3 -4 correct 1
- Below 0
- All 5 correct 2
- 3 -4 correct 1
- Below 0
Conclusion 2
Experiment: # 16
Date:
Title: Excretion
Clarity
Experiment # 17
Date:
Problem statement: Andre went away for a week. When he returned home, he noticed mold was growing on
the bread he left on the kitchen counter. He knows that the mold is a microorganism but
he wanted to find out the conditions/factors that caused the growth of the mold /
microorganism. Plan and design an experiment to find out the factors that cause the
growth of microorganisms.
CRITERIA
● Hypothesis 2
- Clearly stated 1
- Testable 1
● Aim 1
- Related to hypothesis 1
TOTAL = 10 MARKS