9027 Product Sheet - Pseudomonas Paraeruginosa Rudra Et Al.

Pseudomonas
Product Sheet
paraeruginosa Rudra et
al.
9027™
Description
Pseudomonas paraeruginosa strain R. Hugh 813 was isolated from an outer ear
infection. This whole-genome sequenced culture can be used in media testing and
efficacy testing, and it is recommended as a test culture in USP chapters <51>, <60>,
<61>, <62>, and <71>.
Strain designation: R. Hugh 813
Deposited As: Pseudomonas aeruginosa (Schroeter) Migula
Type strain: Yes
Storage Conditions
Product format: Freeze-dried
Storage conditions: 2°C to 8°C
Intended Use
This product is intended for laboratory research use only. It is not intended for any
animal or human therapeutic use, any human or animal consumption, or any
diagnostic use.
BSL 2
ATCC determines the biosafety level of a material based on our risk assessment as
guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories
(BMBL), U.S. Department of Health and Human Services. It is your responsibility to
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Pseudomonas paraeruginosa Rudra et al.
Product Sheet
9027
understand the hazards associated with the material per your organization’s policies
and procedures as well as any other applicable regulations as enforced by your local
or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always

used when handling vials. For cultures that require storage in liquid nitrogen, it is
important to note that some vials may leak when submersed in liquid nitrogen and
will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid
nitrogen back to its gas phase may result in the vial exploding or blowing off its cap
with dangerous force creating flying debris. Unless necessary, ATCC recommends
that these cultures be stored in the vapor phase of liquid nitrogen rather than
submersed in liquid nitrogen.
Certificate of Analysis
For batch-specific test results, refer to the applicable certificate of analysis that can
be found at www.atcc.org.
Growth Conditions
Medium:
ATCC Medium 3: Nutrient agar or nutrient broth
Temperature: 37°C
Atmosphere: Aerobic
Handling Procedures
1. Open vial according to enclosed instructions.
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Product Sheet
9027
2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0

mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
Notes
Both fluorescein and pyocyanin pigments are produced.
Purified genomic DNA of this strain is available (ATCC 9027D-5, ATCC 9027DQ). This
strain is available as Certified Reference Material (ATCC CRM-9027).
Additional information on this culture is available on the ATCC web site at
www.atcc.org.
Material Citation
If use of this material results in a scientific publication, please cite the material in the
following manner: Pseudomonas paraeruginosa Rudra et al. (ATCC 9027)
References
References and other information relating to this material are available at
www.atcc.org.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30
days from the date of shipment, provided that the customer has stored and handled
the product according to the information included on the product information sheet,
website, and Certificate of Analysis. For living cultures, ATCC lists the media
formulation and reagents that have been found to be effective for the product.
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Product Sheet
9027
While other unspecified media and reagents may also produce satisfactory results, a
change in the ATCC and/or depositor-recommended protocols may affect the
recovery, growth, and/or function of the product. If an alternative medium
formulation or reagent is used, the ATCC warranty for viability is no longer valid.
Except as expressly set forth herein, no other warranties of any kind are provided,
express or implied, including, but not limited to, any implied warranties of
merchantability, fitness for a particular purpose, manufacture according to cGMP
standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any
animal or human therapeutic use, any human or animal consumption, or any
diagnostic use. Any proposed commercial use is prohibited without a license from
ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information
on this product sheet, ATCC makes no warranties or representations as to its
accuracy. Citations from scientific literature and patents are provided for
informational purposes only. ATCC does not warrant that such information has been
confirmed to be accurate or complete and the customer bears the sole responsibility
of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and
assumes all risk and responsibility in connection with the receipt, handling, storage,
disposal, and use of the ATCC product including without limitation taking all
appropriate safety and handling precautions to minimize health or environmental
risk. As a condition of receiving the material, the customer agrees that any activity
undertaken with the ATCC product and any progeny or modifications will be
conducted in compliance with all applicable laws, regulations, and guidelines. This
product is provided 'AS IS' with no representations or warranties whatsoever except
as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries,
directors, officers, agents, employees, assigns, successors, and affiliates be liable for
indirect, special, incidental, or consequential damages of any kind in connection with
or arising out of the customer's use of the product. While reasonable effort is made
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Page 4 of 5
Product Sheet
9027
to ensure authenticity and reliability of materials on deposit, ATCC is not liable for
damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the
use of this product. The MTA is available at www.atcc.org.
Copyright and Trademark Information

© ATCC 2023. All rights reserved.
ATCC is a registered trademark of the American Type Culture Collection.
Revision
This information on this document was last updated on 2023-06-10
Contact Information
ATCC
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Email: [email protected] or contact your local distributor
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Pseudomonas

ATCC highly recommends that appropriate personal protective equipment is always

2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0

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