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    Bio Cape P and D

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    winstonbrown1516
    The document outlines a plan to isolate and compare the DNA concentration of strawberries and bananas. It details the materials, method, variables, expected results and sources of error for an experiment where DNA is extracted from each fruit and the volume of precipitate measured to indicate concentration.

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    Bio Cape P and D

    Uploaded by

    winstonbrown1516
    3 views3 pages
    The document outlines a plan to isolate and compare the DNA concentration of strawberries and bananas. It details the materials, method, variables, expected results and sources of error for an experiment where DNA is extracted from each fruit and the volume of precipitate measured to indicate concentration.

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    Bio P & D

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    The document outlines a plan to isolate and compare the DNA concentration of strawberries and bananas. It details the materials, method, variables, expected results and sources of error for an experiment where DNA is extracted from each fruit and the volume of precipitate measured to indicate concentration.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    3 views3 pages

    Bio Cape P and D

    Uploaded by

    winstonbrown1516
    The document outlines a plan to isolate and compare the DNA concentration of strawberries and bananas. It details the materials, method, variables, expected results and sources of error for an experiment where DNA is extracted from each fruit and the volume of precipitate measured to indicate concentration.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Damien Hutchinson

    L6
    P and D
    A molecular biologist interested in investigating the DNA concentration of two
    fruits namely strawberry and banana. Plan and design an experiment to isolate and
    compare the DNA concentration of named fruits.
    Hypothesis: The DNA concentration of the strawberry will be determined to be
    higher than that of the banana due to its’ cellular characteristics and/or genetic
    make up
    Aim: To determine the DNA concertation of the strawberry and banana by
    comparing the volume of their precipitate
    Materials/Apparatus: bananas, strawberries, dishwashing detergent, table salt,
    Isopropyl alcohol(chilled in freezer), two ziplock bags, coffee filter, test tube,
    spoon,electronic balance , knife , beaker, clean container, graduated cylinder
    Method :
    1. Ensure all apparatus are thoroughly cleaned before starting the experiment
    2. Peel and cut the banana into small pieces using a knife
    3. Place individual pieces of cut banana on the electronic balance until a weight
    of 7 grams is obtained
    4. Discard the remaining slices and place the 7 grams of the fruit into a ziplock
    bag
    5. Use a beakerto measure 70 ml of water
    6. Add a pinch of salt and the 70 ml of water to theziplock bag
    7. Use a spoon to mash the fruits in the ziplock bagto a slurry
    8. Measure 3ml of dishwashing liquid using the beakerand add it to ziplock bag
    9. Close the ziplock bag, shake it rigorously then let it sit for 7 minutes
    10.Place the coffee filter over the test tube then pour the liquid from the
    ziplock bag into the test tube to separate the liquid from the solid debris
    11.Transfer the liquid in the test tube into a clean container
    12.Measure 70ml of isopropyl alcohol
    13.Slowly layer chilled isopropyl alcohol on top of the liquid then observe the
    formation of a white, stringy precipitate ( DNA ) at the interface of the
    alcohol and fruit extract
    14.Use a spoon to scoop out the precipitate from container and place in a
    graduated cylinder to obtain the volume
    15.Record the observations made in a table
    16.Repeat steps 1 to 14 for the strawberry
    Data collection table:
    Fruits Volume of Precipitate (cm^3)
    Strawberry
    Banana

    Variables:
    Controlled – the amount of water, dishwashing liquid and grams used for each
    fruit. The time given for each fruit to sit in the ziplockbagsand the amount of
    isopropyl alcohol used on each fruit
    Independent –the fruits used (banana and strawberry)
    Responding – the volume of the precipitate obtained
    Precautions:
     Ensure the graduated cylinder is read at eye level on a flat surface
     Ensure the fruits used were at relative levels of ripeness
    Sources of error:
     Inadequate amount of water or alcohol being used on each fruit
     The precipitate of each fruit was not properly extracted resulting in
    inaccurate values
    Expected results: The volume of the precipitate obtained from the strawberry will
    be greater than that of the banana confirming the proposed hypothesis.

    Explanation of Expected Results:


    The detergent helps break down cell membranes and release cellular contents,
    including DNA, into the solution. Once the DNA is in solution, the addition of
    alcohol causes the DNA to precipitate out of the solution, allowing it to be
    separated from the other cellular components. The combination of these steps
    allows for the extraction and isolation of DNA from the cells of the fruit.The
    volume of the precipitate from the strawberry will be greater than that of the
    banana as strawberries are octoploid meaning they have eight copies of each type
    of DNA chromosome hence yielding more DNA than most other fruits.
    Comparatively bananas are triploid meaning they have three sets of
    chromosomes.This indicates there is a higher probability of the yield of the
    precipitate from the strawberry being higher than that of the banana which may not
    always be the case as DNA concentration is dependent on various other factors not
    strictly accounted for in the experiment like ripeness.

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