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Paper 3

The document provides instructions for a biology practical exam involving various food and plant tests. Students are provided unknown food substances to test for proteins, starch, sugars and vitamins. They also investigate diffusion using a Visking tubing experiment and examine plant specimens under the microscope.

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549 views5 pages

Paper 3

The document provides instructions for a biology practical exam involving various food and plant tests. Students are provided unknown food substances to test for proteins, starch, sugars and vitamins. They also investigate diffusion using a Visking tubing experiment and examine plant specimens under the microscope.

Uploaded by

Denis
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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KISII SCHOOL

Department Of Biological Sciences


231/3 - BIOLOGY - Paper 3
(Practical)
FORM 1 June, 2021 – Time: 13/4 Hours

Name…………………………………….…..........................Admission Number.....................Stream........................

Invigilator ..................................................Signature…................... Date…..........................

Instructions to candidates
 This paper consists of 5 printed pages. Students should check the question paper to ensure
that all pages are printed as indicated and that no questions are missing
 Answer all questions in this paper
 You are required to spend the first 15 minutes of the 1¾ hours allocated for this paper to
read through the questions before beginning the practical.
 Answers must be written in spaces provided on the question paper.
 Additional pages should not be inserted

For Examiner`s Use Only


Question Maximum Score Student’s Score

1 13

2 13

3 14

TOTAL 40

1
1.(a)You are provided with food substance labelled M, use it to carry out the food test and
complete the table below. (8 marks)

Food substance Procedure Observation Conclusion


To 2mls of solution M in a
test-tube, add 3 drops of
Starch Iodine solution and shake

To 2mls of solution M in a
test-tube,add 2mls of
Reducing Sugar Benedict’s solution and
then heat to boil.

To 2mls of solution M in a
test-tube, add 2mls of 10%
Proteins Sodium hydroxide solution
and shake. Into the
mixture, add 1%
Copper(II) Sulphate
solution drop wise as you
shake after each drop.

To2mls of DCPIP in the


test-tube, add solution M
drop wise as you shake
Vitamin C after each drop.

(b)(i) Give one role of any food substance present in solution M (1 mark)
…………………………………………………………………………………………………
(ii) Name the deficiency diseased cause by lack of Vitamin C in the diet (1 mark)
…………………………………………………………………………………………………
(c) Explain the digestion of Starch in the mouth. (3 marks)
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
………………………………………………………………………………………………

2
2.You are provided with avisking tubing, pieces of thread, starch solution and iodine solution in
a beaker. Open one end of the visking and tie tightly using a thread. Pour starch solution into a
visking tubing to about ¾ full. Then tie the other end tightly to ensure than there is no leakage.
Rinse the sides of the tubing with water. Immerse the visking tubing into a beaker containing
Iodine solution. Leave the set up to settle for 20 Minutes.

(a)State the observations made after 20 minutes


(i)Inside the visking tubing (1 mark)
………………………………………………………………………………………………………
(ii)Outside the visking tubing (1 mark)
………………………………………………………………………………………………………
(b)Name the physiological process under investigation (1 mark)
………………………………………………………………………………………………………
(c)Account for the observations made in a above (3marks)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(d) (i)State two factors affecting the process being demonistrated above (2 marks)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(ii) State two roles of the process demonistrated above in living organisms (2 marks)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(e) (i) State the expected observation in the set up above, if the visking tubing was immersed in
distilled water instead of iodine solution (1 mark)
……………………………………………………………………………………………………..
(ii) Account for the observation made in e(i) above (2 marks)
………………………………………………………………………………………………………
……………………………………………………………………………………………………..

3
3 (A)During an ecological study, a form one student collected the specimen shown below for
study.

(i) The scientific name of the above organism is Zea mays. Classify it into its
(a) Kingdom (1 mark)
………………………………………………………………………………………………………
(b) Genus (1 mark)
………………………………………………………………………………………………………
(iii) State two observable features on the specimen that are used for classification (2 marks)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(iv) The magnification of the above specimen was found to be X10. Calculate its actual length in
millimeters. (2marks)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(B) A student then obtained a thin section of the epidermis from part X, placed it on a
microscope slide and then applied a drop of water on it. He then stained it using Iodine solution
and observed the slide under low power and medium power objective lens.
(i) Give reasons why the student carried out the following processes.
(a)Applying a drop of water (1 mark)
………………………………………………………………………………………………………
(b) Applying Iodine solution (1 mark)
………………………………………………………………………………………………………

4
(c) Cutting a thin section (1 mark)
………………………………………………………………………………………………………
(ii) Name any two organelles that a student was able to observe (2 marks)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(iii) Give the formular for calculating magnification when using the light microscope (1mark)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(iv)Give the advantage of using a light microscope at the expense of an electron microscope
when observing specimens (1 mark)
………………………………………………………………………………………………………
………………………………………………………………………………………………………
(v) State the function of the condenser in a light microscope (1 mark)
………………………………………………………………………………………………………

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