PATROL UPLC Process Analyzer System Guide Rev B

PATROL UPLC Process
Analyzer
System Guide
Revision B
Copyright © Waters Corporation 2010

All rights reserved
Copyright notice
© 2010 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF
AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS
DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY
FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and
should not be construed as a commitment by Waters Corporation. Waters
Corporation assumes no responsibility for any errors that may appear in this
document. This document is believed to be complete and accurate at the time
of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use.
Trademarks
ACQUITY, ACQUITY UPLC, NuGenesis, PATROL UPLC, UPLC, and Waters
are registered trademarks of Waters Corporation, and Empower and “THE
SCIENCE OF WHAT’S POSSIBLE.” are trademarks of Waters Corporation.
Phillips is a registered trademark of Phillips Screw Co.
TORX is a registered trademark of Textron, Inc.
Windows is a registered trademark of the Microsoft Corporation.
Other registered trademarks or trademarks are the sole property of their
owners.
ii
Customer comments
Waters’ Technical Communications department invites you to tell us of any
errors you encounter in this document or to suggest ideas for otherwise
improving it. Please help us better understand what you expect from our
documentation so that we can continuously improve its accuracy and
usability.
We seriously consider every customer comment we receive. You can reach us
at [email protected].
Contacting Waters
®
Contact Waters with enhancement requests or technical questions regarding
the use, transportation, removal, or disposal of any Waters product. You can
reach us via the Internet, telephone, or conventional mail.
Waters contact information:
Contacting medium Information

Internet The Waters Web site includes contact
information for Waters locations worldwide.
Visit www.waters.com.
Telephone and fax From the USA or Canada, phone 800
252-HPLC, or fax 508 872-1990.
For other locations worldwide, phone and fax
numbers appear in the Waters Web site.
Conventional mail Waters Corporation
34 Maple Street
Milford, MA 01757
USA
Safety considerations
Some reagents and samples used with Waters instruments and devices can
pose chemical, biological, and radiological hazards. You must know the
potentially hazardous effects of all substances you work with. Always follow
iii
Good Laboratory Practice, and consult your organization’s safety
representative for guidance.
When you develop methods, follow the “Protocol for the Adoption of Analytical
Methods in the Clinical Chemistry Laboratory,” American Journal of Medical
Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating
procedures and the techniques necessary to validate system and method
performance.
Considerations specific to the PATROL system

See also:
®
• For additional safety information about ACQUITY components used
with the PATROL system, consult the PATROL documentation CD.
• For specific warnings regarding the TUV detector, consult the TUV
detector maintenance section of the ACQUITY UPLC System Operator’s
Guide.
• For specific warnings regarding the PDA and eλPDA detectors, consult
the ACQUITY UPLC Photodiode Array and eλPhotodiode Array Detector
Operator’s Overview and Maintenance Guide.
Warning: To avoid injury from various causes, allow only

Waters-trained personnel (with access to the inside of the enclosure) to
install the system and its enclosure and to perform service and
maintenance operations.
Warning: To avoid contact with hazardous substances, wear clean,

chemical-resistant, powder-free gloves when handling tubing and other
parts of the system that come in contact with solvents.
Warning: To avoid personal exposure to biologically hazardous agents,

do not use the system to analyze such materials, or allow them to come
into contact with the system or its enclosure.
Warning: To avoid injury, spills, and damage to the equipment, remove

all instrument modules, solvent containers, and waste containers before
relocating the PATROL system enclosure. Do not relocate the PATROL
system enclosure with instruments or reservoirs installed.
iv
Warning: To avoid igniting solvents, do not use an electrical stirrer
inside the enclosure.
Warning: To avoid electric shock and damage to the equipment, ensure

that the circuit breakers are in the off position before connecting the AC
power cord to a receptacle.
Warning: To avoid exposure to harmful vapors, connect an exhaust

system or other apparatus to the flanged duct (vent) on the top
surface of the enclosure capable of evacuating vapors released
from the PATROL system.
Warning: To avoid electric shock, use extreme caution when working in

areas where you see this sign:
Warning: To avoid exposure to laser radiation, use caution when

performing maintenance operations involving the process sample
manager’s internal bar-code reader. The bar code reader utilizes a
Class 1 laser.
Warning: To avoid injury and damage to the enclosure, do not exert a

downward force greater than the weight of one computer keyboard on
the process sample manager access door when it is open.
Caution: To ensure proper drainage, verify that the waste management

system for the ACQUITY modules is fully aligned.
Requirements:
• Ensure that a master AC disconnect is located near the system and is
easily accessible to system users.
v
• Ensure that use of the PATROL system, including any emergency
procedures, is consistent with the safety practices followed by your
organization. If a serious malfunction occurs that requires you to
®
immediately remove AC power from the PATROL UPLC Process
Analyzer system, press the emergency-stop button located on the front of
the options compartment.
• Locking the PATROL system enclosure restricts access to system
components and reduces the safety risks of users who have not received
specific training from Waters on performing tasks within the enclosure.
Users who have been trained by Waters to perform tasks inside the
enclosure, must observe all safety warnings and cautions associated
with the PATROL UPLC Process Analyzer system and enclosure.
• For installations outside of the United States, a grounded AC plug must
be provided by the customer.
Safety advisories
Consult Appendix A for a comprehensive list of warning and caution
advisories.
Disposal
Do not dispose of instrument components in municipal waste.
Dispose of the PATROL UPLC Process Analyzer system according to local,
state, provincial, federal, and international regulations.
Contact Waters with questions regarding the transportation, removal, or
disposal (consistent with the WEEE Directive) of any Waters product.
Operating this system

When operating this system, follow standard quality-control (QC) procedures
and the guidelines presented in this section.
vi
Applicable symbols
Symbol Definition
Authorized representative of the European
Community
Confirms that a manufactured product complies

with all applicable European Community
directives
ABN 49 065 444 751 Australia C-Tick EMC Compliant
Confirms that a manufactured product complies

with all applicable United States and Canadian
safety requirements
This product has been tested to the requirements
of CAN/CSA-C22.2 No. 61010-1, second edition,
including Amendment 1, or a later version of the
same standard incorporating the same level of
testing requirements
Audience and purpose

This document is for personnel who work in a manufacturing environment. It
includes procedures for operating and maintaining the PATROL system as a
tool for QC/QA analysis.
Intended use of the PATROL system

Waters designed the PATROL UPLC Process Analyzer to analyze in-process
and final-production samples in a manufacturing environment.
Warning: Do not use the PATROL UPLC Process Analyzer in a manner

not specified in this guide or that is otherwise inconsistent with the
instrument's design. Doing so could defeat or impair the function of
various systems and devices designed to protect the operator from
personal injury.
vii
Calibrating
To calibrate LC systems, follow acceptable calibration methods using at least
five standards to generate a standard curve. Include the entire range of QC
samples, typical specimens, and atypical specimens in the concentration range
for standards.
When calibrating mass spectrometers, consult the calibration section of the
operator’s guide for the instrument you are calibrating. In cases where an
overview and maintenance guide, not operator’s guide, accompanies the
instrument, consult the instrument’s online Help system for calibration
instructions.
Quality control
Routinely run three QC samples that represent subnormal, normal, and
above-normal levels of a compound. Ensure that QC sample results fall within
an acceptable range, and evaluate precision from day to day and run to run.
Sometimes, data collected when QC samples are out of range is invalid. Do not
report these data until you are certain that the instrument performs
satisfactorily.
ISM classification
ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with IEC CISPR 11
Industrial Scientific and Medical (ISM) instruments requirements. Group 1
products apply to intentionally generated and/or used conductively coupled
radio-frequency energy that is necessary for the internal functioning of the
equipment. Class B products are suitable for use in both commercial and
residential locations and can be directly connected to a low voltage,
power-supply network.
viii
EC Authorized Representative
Waters Corporation (Micromass UK Ltd.)

Floats Road
Wythenshawe
Manchester M23 9LZ
United Kingdom
Telephone: +44-161-946-2400
Fax: +44-161-946-2480
Contact: Quality manager
ix
x
Table of Contents
Copyright notice ................................................................................................... ii
Trademarks ............................................................................................................ ii
Customer comments ............................................................................................ iii
Contacting Waters ............................................................................................... iii
Safety considerations .......................................................................................... iii

Considerations specific to the PATROL system................................................ iv
Safety advisories ................................................................................................. vi
Disposal ............................................................................................................... vi
Operating this system ......................................................................................... vi

Applicable symbols ........................................................................................... vii
Audience and purpose....................................................................................... vii
Intended use of the PATROL system............................................................... vii
Calibrating ....................................................................................................... viii
Quality control ................................................................................................. viii
ISM classification .............................................................................................. viii

ISM Classification: ISM Group 1 Class B ...................................................... viii
EC Authorized Representative ......................................................................... ix
1 Analyzing Manufacturing Processes with the PATROL System 1-1

System technology ............................................................................................ 1-2
System components .......................................................................................... 1-2

Installation of the PATROL UPLC Process Analyzer system, enclosure, and
components................................................................................................. 1-5
System enclosure ............................................................................................. 1-6
ACQUITY components .................................................................................... 1-8
Process sample manager ................................................................................. 1-9
Computer and touch screen........................................................................... 1-11
Table of Contents xi
Accessing the system through the software interface ........................... 1-11
Determining tasks allowed for a user level .............................................. 1-11
2 Configuring the System ........................................................................ 2-1

Configuring PATROL software ..................................................................... 2-2
Creating and modifying methods.................................................................... 2-3
Specifying a decision-maker file...................................................................... 2-3
Configuring timeouts ....................................................................................... 2-4
Logging in to Empower software from the touch screen ........................ 2-4
3 Running Samples ................................................................................... 3-1

Introducing a bar-coded sample vial into the system .............................. 3-2
Running priority samples................................................................................ 3-4
Loading standards ............................................................................................ 3-4
Unloading an accepted, analyzed, or rejected vial ................................... 3-5
Configuring methods ....................................................................................... 3-5
4 Maintaining the System ........................................................................ 4-1

Maintenance schedule ..................................................................................... 4-2
Configuring maintenance warnings ............................................................. 4-3
Spare parts ......................................................................................................... 4-4
Maintenance considerations .......................................................................... 4-6

Safety and handling......................................................................................... 4-6
Powering on the system................................................................................... 4-6
Shutting down the system............................................................................... 4-7
Accessing or removing instrument components ....................................... 4-8

Disconnecting the process sample manager................................................... 4-9
Sliding an instrument forward or removing it from the enclosure ............... 4-9
xii Table of Contents

Adding or replacing solvents ....................................................................... 4-11
Emptying waste containers .......................................................................... 4-11

Switching columns ......................................................................................... 4-12
Relocating the system .................................................................................... 4-12
Maintaining the process sample manager ................................................ 4-13

Online valve control....................................................................................... 4-13
Priming the online flow path......................................................................... 4-14
Running a leak test........................................................................................ 4-15
Calibrating the needle seal ........................................................................... 4-16
Upgrading firmware ...................................................................................... 4-17
Resolving leak sensor errors ......................................................................... 4-17
Replacing the process sample manager’s leak sensor.................................. 4-23
Replacing the sample loop ............................................................................. 4-26
Replacing pumps and valves ......................................................................... 4-30
Replacing the mixer ....................................................................................... 4-43
Cleaning the filters ........................................................................................ 4-44
Replacing the fuses ........................................................................................ 4-45
Cleaning the system enclosure’s exterior ................................................. 4-47
A Safety Advisories .................................................................................. A-1

Warning symbols ............................................................................................... A-2
Task-specific hazard warnings........................................................................ A-2
Specific warnings ............................................................................................. A-3
Caution symbol .................................................................................................. A-4

Warnings that apply to all Waters instruments ......................................... A-4
Electrical and handling symbols ................................................................. A-11

Electrical symbols .......................................................................................... A-11
Handling symbols .......................................................................................... A-12
Table of Contents xiii

B Materials of Construction and Compatible Solvents ................... B-1
Preventing contamination ............................................................................. B-2
Items exposed to solvent ................................................................................ B-2

Solvents used to prepare mobile phases .................................................... B-2
C Specifications ........................................................................................ C-1

Specifications for the PATROL UPLC Process Analyzer ....................... C-2
Specifications for the process sample manager ....................................... C-3
xiv Table of Contents

1 Analyzing Manufacturing
Processes with the PATROL
System
Waters designed the PATROL UPLC Process Analyzer for use on the
production floor, performing QC and QA analysis. The system enables
real-time, quantitative analysis of in-process and final-production
samples. It analyzes samples introduced by a walk-up user or aspirated
directly from a manufacturing process stream.
Contents:
Topic Page
System technology 1-2
System components 1-2
Accessing the system through the software interface 1-11
Determining tasks allowed for a user level 1-11
1-1
System technology
The PATROL system is a process analytical technology (PAT) system. Its
UPLC® technology produces real-time, quantitative results. The PATROL
®
system is part of the same technology platform as Waters’ ACQUITY UPLC
System. The system performs process sampling, sample handling,
chromatographic analysis, and data management tasks.
A system enclosure facilitating walk-up sample submission houses the system
components.
To use the system, you introduce individual, bar-coded vials to the process
sample manager, which then reads the bar codes, accepts or rejects the vials,
and runs the specified test procedures. You can also configure the process
sample manager to aspirate samples directly from a manufacturing process
stream.
The modular design of the PATROL system’s components lends itself to fast
and efficient maintenance and support.
System components
The PATROL UPLC Process Analyzer system comprises the following
elements:
• ACQUITY binary solvent manager
• ACQUITY column manager (CM-A)
• ACQUITY auxiliary column manager (CM-Aux), optional
• ACQUITY detector (TUV, PDA, or eλPDA)
• ACQUITY process sample manager (PSM)
• Leak sensors
• Computer, with touch screen and keyboard
™
• PATROL and Empower software
• Solvent and waste storage
• Solvent recycling valve module, optional
• Options compartment
1-2 Analyzing Manufacturing Processes with the PATROL System

• System enclosure (vented, with a status-indicating light tower and
emergency stop button)
System enclosure:
Light tower
Vent
Emergency-stop
button (e-stop)
Instrument
compartment
Options
compartment
Computer touch
screen
Solvent and waste

compartment
Sample access
door (to access
process sample
manager)


Connections to the top of the enclosure:
Light tower Vent
Ethernet
Instrument air
AC power
See also: For more information on connections to the enclosure, see the
PATROL UPLC Process Analyzer Site Preparation Guide.
System components:
Light tower
E-stop
Detector Circuit
breakers
Auxiliary DC supply
column
manager System PC
Column Discrete I/O

manager panel
Process
sample
manager Solvent
reservoirs
Binary
solvent
manager
Waste
reservoirs
(not shown)
Recycle valve
(not shown)

Installation of the PATROL UPLC Process Analyzer system,
enclosure, and components

system enclosure with instruments or reservoirs installed.
Warning: To avoid electric shock and damage to the equipment, ensure

that the circuit breakers are in the off position before connecting the AC
power cord to a receptacle.
Warning: To avoid exposure to harmful vapors, connect an exhaust

system or other apparatus to the flanged duct (vent) on the top
surface of the enclosure capable of evacuating vapors released
from the PATROL system.

areas where you see the following sign:
Caution: To ensure proper drainage, verify that the waste management

system for the ACQUITY modules is fully aligned.
Requirement: For installations outside of the United States, a grounded AC

plug must be provided by the customer.

See also: For additional details about connecting an exhaust system, consult
the PATROL UPLC Process Analyzer System Site Preparation Guide.
Note: During installation, the fan that is part of the thermal management
system is set to the minimum speed necessary for successful thermal
management and the evacuation of vapors.
System enclosure
The system enclosure houses three adjoining compartments: instrument,
solvent and waste, and options. Each compartment is fitted with a separate
front-panel door, which you can secure using a locking mechanism, if desired.
Requirement: The front-panel doors interlock such that the solvent and waste
compartment door must be opened before opening either the instrument or
options compartment doors.
Tip: System administrators use the separate doors and locking mechanisms to
restrict access to specific user types.
Requirement: Locking the PATROL system enclosure restricts access to
system components and reduces the safety risks of users who have not
received specific training from Waters on performing tasks within the
enclosure. Users who have been trained by Waters to perform tasks inside the
enclosure, must observe all safety warnings and cautions associated with the
PATROL UPLC Process Analyzer system and enclosure.
The enclosure includes a light tower on the top surface to indicate system
status:
Light color Indication

Red Error – An error has occurred. The current sample set
aborts and the system goes offline.
Yellow Warning – Continuing system operation exceeds a
maintenance counter threshold or a sample vial has
been rejected.
Green Normal operation – The system is ready to accept
samples.
An emergency-stop button (e-stop) is located on the front of the options

compartment. When pushed, the e-stop removes AC power from the system.

For information about using the e-stop, see “Considerations specific to the
PATROL system” on page iv.
Thermal management within the enclosure is accomplished by forced-air
convection. An air intake vent is located on the bottom surface of the enclosure
and a vent for exhaust is located on the top surface of the enclosure. This
system also helps to remove vapors from the enclosure.
Note: During installation, the fan that is part of the thermal management
system is set to the minimum speed necessary for successful thermal
management and the evacuation of vapors.
Instrument compartment
Inside the instrument compartment (left-hand side of the enclosure), each of
the system components rests on a sliding drawer so that, when the
compartment door is fully open, you can slide the components forward,
enabling access to panels and connections.
Note: An optional solvent recycling valve module is also located in the
instrument compartment if it is included as part of the system.
When the main instrument compartment door is closed, you can open a
smaller entry door to directly access the process sample manager from the
front of the enclosure.

Solvent and waste compartment

Inside the solvent and waste compartment, the solvent supply and waste
containers occupy secondary containment receptacles. The solvent supply is
located on the top (sliding) drawer and the waste containers on the bottom
shelf.
Tubing for the analysis solvent exits the compartment through a manifold.
Waste tubing enters through two separate waste outlets from the instrument
compartment.

Requirement: Use only the combinations of solvent bottle sizes listed in the
table, below:
Acceptable configurations for solvent reservoir bottles:
Configuration A Configuration B Configuration C

Reservoir
volumes volumes volumes
Solvent A1 2000 mL 5000 mL 5000 mL
Solvent A2 2000 mL – 5000 mL
Solvent B1 2000 mL 5000 mL 1000 mL
Solvent B2 2000 mL – 1000 mL
Diluent 1000 mL 1000 mL 1000 mL
Seal Wash 500 mL 500 mL 500 mL
Warning: To avoid spills, ensure that the waste container volume is

large enough to contain the total volume of all solvents in use on the
system.
Warning: To avoid igniting solvents, do not use an electrical stirrer

inside the enclosure.
Note: From the solvent and waste compartment, you can access a regulated
instrument air supply (758 kPa/110 psig/7.58 bar maximum pressure). You
can use the instrument air to supply an ancillary laboratory device (a
pneumatic stir plate for chromatographic solvents, for example), if desired.
See the PATROL UPLC Process Analyzer Site Preparation Guide for
additional details.
Options compartment
Authorized users can access the options compartment to perform installation
and maintenance functions.
ACQUITY components
The system uses four ACQUITY UPLC components (the binary solvent
manager, column manager, auxiliary column manager, and detector) along
with a process sample manager specific to the PATROL system. See the

PATROL documentation CD for information about operating and maintaining
ACQUITY UPLC components.
Process sample manager

Front and rear view of the process sample manager:
Power button
and LEDs
Access panel
Fuse holder
Power connection
Ethernet connection
Sample door
(for vials)
Access panel
Leak tray with

leak detector
Front Rear
You can submit samples by using the process sample manager. When you
insert a bar-coded vial through the process sample manager’s door and into
the sample carousel, the process sample manager reads the bar code to verify
sample identity. It then performs any required dilutions and injects the
sample mixture onto the appropriate analysis column (located in the column
manager or auxiliary column manager). You can also configure the process
sample manager to aspirate samples directly from a manufacturing process
stream.

Process sample manager’s flow path:
Process line #2
Process line #3
Process line #6
Process line #1
Process line #5
Sampling
valve
Priming
valve Injection
valve
Plugged
Waste
To column
Transducer
Transducer
Sensor manager
Waste Mixer
10 μL To binary
Diluent Process solvent
pump manager
Process
valve
Wash Needle drive

Process
Diluent Sample line #4
pump pump
Wash waste
Wash
Vial
Injection
port Wash pump

Tip: The process sample manager can perform only full-loop injections using a
1-μL, 2-μL or 5-μL sample loop.
The sample carousel holds 32, 2-dram vials. Inserted samples and standards
remain in the carousel until you remove them. The process sample manager
controls (locks and unlocks) the entry door, limiting to one at a time your
ability to introduce and remove samples, and preventing unauthorized users
from accessing the carousel.
The control-panel buttons and front-panel LEDs of the process sample
manager function as they do on standard ACQUITY components. (See the
ACQUITY UPLC System Operator’s Guide on the PATROL documentation
CD.) You control the process sample manager’s instrument parameter values
via the Empower™ software method editor and the ACQUITY UPLC Console
software.
Computer and touch screen

A computer, located inside the options compartment, runs the Windows®
operating system, the PATROL system software, and Empower software to
control the PATROL system. A user can interact with the PATROL computer
via the touch screen mounted in the front-panel door of the instrument
compartment, or by connecting to it by using Windows Remote Desktop
Connection software.
Accessing the system through the software interface

The PATROL system software is a collection of components that coordinates
loading and aspirating samples, creating and executing Empower sample sets,
®
and exporting results to the Waters NuGenesis Scientific Data Management
System (SDMS).
The system software loads when the Windows operating system starts,
enabling you to interact with the system interface via the touch screen on the
outside of the enclosure. You must log in to Windows and Empower software
to access advanced software functions.
Determining tasks allowed for a user level

There are two types of PATROL system users:
Accessing the system through the software interface 1-11

• Users who have walk-up access, but no access to components inside the
system enclosure.
• Users who have access to system components located inside the system
enclosure.
Requirements:
• Users who have access to the inside of the enclosure must receive
additional training from Waters to maintain the PATROL UPLC
Process Analyzer system and enclosure. Contact Waters for more
information.
• Locking the PATROL system enclosure restricts access to system
components and reduces the safety risks of users who have not
received specific training from Waters on performing tasks within
the enclosure. Users who have been trained by Waters to perform
tasks inside the enclosure, must observe all safety warnings and
cautions associated with the PATROL UPLC Process Analyzer
system and enclosure.
Your Empower user type determines your level of system software access.
Without a Windows or Empower login, you can perform only limited tasks
using the basic operator interface. Contact your system administrator for a
list of tasks allowed for your user type.

2 Configuring the System
The PATROL system administrator configures instrument software and

user accounts in the Empower software at installation. Users given
log-in access can perform some advanced tasks. See the Empower
software online Help or the Empower 2 System Administrator’s Guide
for information on software administration.
Contents:
Topic Page
Configuring PATROL software 2-2
Logging in to Empower software from the touch screen 2-4
2-1
Configuring PATROL software
After the instrument control software for the PATROL system is installed, a
system administrator must configure a PATROL system in the Empower
software at system installation. Consult the Empower online Help or the
Empower 2 System Administrator’s Guide for details on how to configure a
new chromatographic system.
To configure a PATROL system:

1. Verify that the PATROL software (PATROL Process Analyzer
November 2010 Driver Pack, for example) is installed.
2. Log in to Empower software from the Windows desktop.
3. From the Run Samples window, launch the ACQUITY Console.
4. From the ACQUITY UPLC Console window, click PATROL in the
instrument tree.
5. Click Configure > System.
6. Select a database and project, enter a valid user name and password for
the system, and then click Next.
Tip: The software acquires data using the login account (Empower) you
enter and stores data in the project and database you specify.
7. Select appropriate PATROL decision makers for your configuration (see
“Specifying a decision-maker file” on page 2-3) and a PATROL system.
8. Select Enable Screen Saver if using the touch-screen system interface is
desired, and then click Next.
Important: The status-indicating light tower does not function when the
touch-screen system interface is disabled.
9. Verify that the sample status file name, configuration file name, file
access parameter values, bar-code format, conditioning step, and
configuration table entries are correct. Make changes, if needed.
10. In the configuration table, select method sets for your samples, and then
click Next or Finish.
Requirement: You must select method sets and reconfigure standards
whenever you change projects (see“Loading standards” on page 3-4).
2-2 Configuring the System

11. If you are configuring online parameters, enter or select appropriate
Command, Action, and Sample Set information, and then click Finish.
12. Exit Empower software and restart Windows on the system PC.
Creating and modifying methods

Authorized users can create and modify methods in the Empower software for
use in the configuration table of the decision-maker file (see “Specifying a
decision-maker file” on page 2-3).
See also: Empower online Help for information on creating and modifying
analysis and report methods.
Specifying a decision-maker file

Atline decision-maker file
The atline decision-maker file that the administrator chooses when
configuring a PATROL system includes sample status and identification and
assigns analysis methods to specific samples (or process steps). It also
determines the rules for the process steps and whether a sample can run.
PATROL system administrators specify an atline decision-maker file for each
system at installation, if appropriate. The administrator chooses the file as
part of the software configuration. Only authorized users can change the
decision-maker file assigned to a PATROL system.
The atline default decision-maker file specifies the following information:
• Sample status – A table of accepted samples stating whether a sample
has been run, or is currently running, and on which system.
• Configuration – A table matching the process step (sample ID from the
bar code), with the sample description and information about general
analysis parameter values. The administrator can specify a file name for
a new configuration file, or browse and select a file that already exists.
In the configuration file, the administrator specifies parameter values
for each process step that the software uses to create an Empower
sample set. The parameters associated with each process step/sample ID
are sample name, dilution, method set, and run time.
Configuring PATROL software 2-3

Online decision maker file
The online decision-maker file specifies and describes commands that an
external controller uses to conduct the analysis of samples aspirated directly
from a process stream. The online decision-maker file also specifies which
sample set to use when executing the commands.
Configuring timeouts
The process sample manager requires you to complete certain tasks within a
specified time interval. An authorized PATROL user can configure intervals
(timeouts) that allow these operations:
• Insertion or removal of a sample or standard
• Removal of a rejected sample or standard
To configure timeouts:
1. In the process sample manager information window, click Configure >
Timeouts.
2. Specify appropriate time intervals for each operation.
Logging in to Empower software from the touch screen

You can access the Empower software by logging in to the Windows operating
system through the PATROL touch-screen interface.
Alternative: Log in to the system using Windows Remote Desktop Connection
to access Empower software.
To log in to Empower software from the touch screen:

1. Touch Tech in the lower right-hand corner of the screen.
2. Enter a valid Empower user name and password, and then touch OK.
3. In the window that appears, touch Exit to Windows.
4. Enter a valid Windows user name and password, and then touch OK.
5. On the Windows desktop, double-touch the Empower icon.
6. Enter a valid Empower user name and password, and then click OK.

Requirement: A PATROL system must be configured in the Empower
software at system installation. Otherwise, consult your system
administrator.
Logging in to Empower software from the touch screen 2-5

3 Running Samples
The PATROL system enables walk-up sample submission and

automated sampling from manufacturing process streams.
Alternatively, you can run samples remotely, using the Empower
software.
Contents:
Topic Page
Introducing a bar-coded sample vial into the system 3-2
Loading standards 3-4
Unloading an accepted, analyzed, or rejected vial 3-5
Configuring methods 3-5
3-1
Warning: To avoid spills and inadequate solvent supply, verify daily
that fluid reservoirs are at an appropriate level. Mobile-phase and
liquid-waste counters are not currently active.
Caution: To avoid delays in testing, you can configure the system to

notify users when the carousel occupancy reaches a specified level. If the
carousel is full (32 vials) and you attempt to load a vial, the system
displays a status of “idle”.
Caution: To ensure reliable temperature control, do not set the process

sample manager’s sample compartment temperature more than 20 °C
below the temperature in the instrument compartment of the enclosure.

areas where you see the following sign:
Introducing a bar-coded sample vial into the system

Sample vials must display an acceptable, bar-coded label (Waters part
number 615004117). They must also be correctly sized and have a septum and
cap (2 dram, Waters part number 186004903C).
3-2 Running Samples

Caution: To ensure that the bar-code reader performs reliably, verify
that bar-coded labels have the following characteristics:
• A width of 0.75 inch and a height of 2.25 inches
• Use bar-code symbology code 39 with extended character set
• Use a code of 7 to 16 characters
• Are composed of weatherproof polyester suitable for laser printing
• Are applied to the vial so that the bar-code lines are perpendicular to
the vertical axis of the vial (and are no more than 10° askew).
Properly applied bar-coded label:
To run a sample, initiate the vial loading process via the touch screen.
Introducing a bar-coded sample vial into the system 3-3

To load a vial:
1. Touch the green Load Samples button on the PATROL status screen.
Tip: If the system is busy, a message appears stating that the system
will complete the current operation before proceeding.
2. When instructed, open the sample door of the process sample manager,
insert a correctly oriented vial into the carousel, and then close the
sample door.
Result: The software notifies you of the time allowed to complete the
action and whether the sample is accepted or rejected. If the vial is
accepted, the software prompts you to insert another.
3. Touch Done when no vials remain to insert.
Requirement: If a sample is rejected, you must remove it before the
displayed time interval ends and the system takes custody of the vial
and notifies a system administrator about the error.
Running priority samples

The PATROL system can recognize high-priority samples inserted into the
sample carousel (see “To load a vial:” on page 3-4). The bar code on the label of
such samples identifies them as high priority, and they run immediately or
after the completion of a sample and control pair if one is currently running.
Important: Samples aspirated directly from a process stream are given highest
priority.
Loading standards
You must log in to the PATROL system to load standard vials.
To load standards:
1. Touch the Tech button in the lower right-hand corner of the PATROL
touch screen.
2. Enter a valid password, and then touch OK.
3. In the window that appears, touch Insert Standards.
4. Specify the vial position, method set, volume, and threshold for the
standard.
3-4 Running Samples

5. Select which process steps will use the standard, and then touch OK.
Result: A message appears instructing you to insert the standard vial
during a specified time interval.
6. Open the sample door, insert the vial, close the sample door within the
specified time interval, and touch Done.
Unloading an accepted, analyzed, or rejected vial

You must remove rejected sample vials from the sample carousel within a
configurable time interval after their rejection (30 seconds, for example).
To remove an accepted or analyzed vial from the PATROL sample carousel,
you must have the Empower login privilege.
To remove an accepted or analyzed vial:

1. From the PATROL touch screen, touch the Tech button in the lower
right-hand corner.
2. Enter a valid password, and then touch OK.
3. In the window that appears, touch View Carousel.
Alternative: After logging in, touch Remove Vials, to remove all of the
vials in the carousel, one at a time.
4. In the window that appears, locate the information about the vial (or
vials) you are removing.
5. Mark the boxes that correspond to the positions of the vials you are
removing, and then click Remove vial or vials.
Result: When the vial being removed is identified, the system allows a
specified interval during which you must remove the sample.
6. Open the sample door, remove the sample, close the sample door within
the specified interval, and touch Done.
Configuring methods
You can configure instrument methods in Empower by using the process
sample manager’s instrument method editor.
Unloading an accepted, analyzed, or rejected vial 3-5

From the General tab of the editor, you can choose parameter values for
washes, sample temperature, and dilutions. You can also set parameter
values for online process sampling. Advanced options allow you to specify the
syringe draw rate and the distance of the sample needle from the bottom of
sample vials.
General tab of the instrument method editor:
Advanced options from the General tab:
3-6 Running Samples

From the Data tab of the editor, you can select which data channels to acquire
for your system.
Data tab of the instrument method editor:
Additional channels are available to use for diagnosing problems with the
system.
Diagnostic channels available from the Data tab:
Configuring methods 3-7

3-8 Running Samples
4 Maintaining the System
The system enclosure facilitates access to instruments and other system

components for maintenance or replacement purposes. For routine
operation, you can access the solvent storage and waste receptacles
without accessing the instrument compartment.
Contents:
Topic Page
Maintenance schedule 4-2
Configuring maintenance warnings 4-3
Spare parts 4-4
Maintenance considerations 4-6
Accessing or removing instrument components 4-8
Adding or replacing solvents 4-11
Emptying waste containers 4-11
Switching columns 4-12
Relocating the system 4-12
Maintaining the process sample manager 4-13
Cleaning the system enclosure’s exterior 4-47
4-1
Maintenance schedule

For details about the routine maintenance of the binary solvent manager,
column manager, auxiliary column manager, TUV detector, PDA detector, or
eλPDA detector, consult the ACQUITY UPLC System Operator’s Guide and
the appropriate column compartments or detector information (on the
PATROL documentation CD).
Waters recommends that you perform the following routine maintenance on
the process sample manager to ensure reliable operation and accurate results.
Recommended routine maintenance schedule for the process sample

manager:
For
Maintenance procedure Frequency
information...
Resolving leak sensor errors As needed See page 4-17
Replacing the leak sensor As needed See page 4-23
Replacing a sample loop When the See page 4-26
injection valve is
replaced during
scheduled
preventative
maintenance, or
as needed
Replacing the injection valve cartridge During scheduled See page 4-31
preventative
maintenance
Replacing the priming valve cartridge During scheduled See page 4-31
preventative
maintenance
4-2 Maintaining the System

Recommended routine maintenance schedule for the process sample
manager:
For
Maintenance procedure Frequency
information...
Replacing the sampling valve During scheduled See page 4-31
cartridge preventative
maintenance
Replacing the process valve cartridge During scheduled See page 4-31
preventative
maintenance
Replacing the sample pump During scheduled See page 4-36
preventative
maintenance, or
as needed
Replacing the diluent pump During scheduled See page 4-39
preventative
maintenance
Replacing the process pump and As needed See page 4-41
installing the tubing
Replacing the mixer As needed (see See page 4-43
tip, below)
Calibrating the needle seal As needed See page 4-16
Cleaning the air filters During scheduled See page 4-44
preventative
maintenance, or
as needed
Replacing the fuses As needed See page 4-45
Tip: Replace the mixer if the sample/wash pressure or diluent pressure

remains above 500 psi during normal use.
Configuring maintenance warnings

Maintenance counters provide real-time usage status information that can
help you determine when to schedule routine maintenance for specific
components. You can set usage thresholds and maintenance warnings that
alert you when a component reaches the designated threshold limit. By
Configuring maintenance warnings 4-3

setting threshold limits and monitoring these usage counters regularly, you
can minimize unexpected failures and unscheduled downtime during
important work.
For information on setting maintenance warnings for the binary solvent
manager, column manager, or detector, consult the ACQUITY UPLC Console
online Help.
A maintenance warning can be set for the number of process sample manager
injection cycles. The process sample manager requires regular maintenance,
the frequency of which depends on how many injections it makes. From the
Maintenance Counter window of the ACQUITY UPLC Console software, you
can set a maintenance warning to alert you when the injection count reaches a
specified number.
To set the injection count warning:

1. In the Maintenance Counter window of the ACQUITY UPLC Console
software, click the indicator bar.
Alternative: Click Process Sample Manager in the instrument tree, and
then click the injections indicator bar in the Console window.
2. Check Enable Sample Manager injections warning level, and enter the
number of injections.
3. Click the green check mark.
To reset the injection counter:

1. In the ACQUITY UPLC Console window, click Process Sample Manager
in the instrument tree.
2. Click Maintain > Reset injection count.
3. In the window that appears, click Yes to reset the counter.
Spare parts
Replace only the component parts mentioned in this document and in the
ACQUITY UPLC System Operator’s Guide (on the PATROL documentation
CD). See the Waters Quality Parts Locator on the Waters Web site Services &
Support page.

Spare parts for the process sample manager
The following spare parts are available for maintaining the process sample
manager.
Note: There are no customer serviceable parts of the PATROL system
enclosure.
Spare parts for the process sample manager:
Part number Description

700002765 ASSY, VALVE, INJECTOR (Injection valve cartridge)
700002660 ASSY, VALVE, VENT (Priming valve cartridge)
700005236 ASSY, VALVE, INJECTOR, CPSM (Sampling valve
cartridge)
700005408 ASSY, VALVE, SOLVENT SELECT (Process valve cartridge)
700005140 ASSY, PUMP MOTOR, DILUTE (Diluent pump)
700005141 ASSY, PUMP MOTOR, SAMPLE (Sample pump)
700005543 PUMP, PERISTALTIC, 114DVP (Process pump)
700005544 TUBING, PERI PUMP, .03 ID (Process pump tubing)
700005557 Needle, 0.058, 100 DEG (Sample needle, 0.058-inch OD)
430001516 ASSY, SAMPLE LOOP, 1uL (Sample loop, 1 μL)
430001264 ASSY, 2uL SAMPLE LOOP, GROUND (Sample loop, 2 μL)
430001311 ASSY,SAMPLE LOOP, 5UL (Sample loop, 5 μL)
700005139 FUSE, 10A 250V FAST ACTING 5X20 (Replacement fuse)
205000505 KIT, LEAK SENSOR ASSEMBLY (Leak sensor kit)
700005133 FILTER, AIR INTAKE, PSM (Air filter for PSM)
615004117 PATROL sQT Bar Code Labels (Vial labels for bar codes)
186004903C 15 × 75 Glass, Screw Cap, preslit Septa (Sample vials)
Spare parts 4-5

Maintenance considerations
Safety and handling

Observe these warning and caution advisories when you perform maintenance
operations on your system.

Warning: To prevent injury, always observe Good Laboratory Practices

when you handle solvents, change tubing, or operate the system. Know
the physical and chemical properties of the solvents you use. See the
Material Safety Data Sheets for the solvents in use.
Warning: To avoid contact with hazardous substances, wear clean,

chemical-resistant, powder-free gloves when handling tubing and other
parts of the system that come in contact with solvents.
Warning: To avoid electric shock, do not remove an instrument’s

protective panels unless instructed. The components within are not
user-serviceable. Use extreme caution when working in areas of the
enclosure where you see the following sign:
Powering on the system

To power on the system (after a non-emergency shutdown):
1. Verify that all system instruments are powered off.
2. Set the master AC disconnect control to the “on” position.

3. Set the circuit breakers to the “on” position.
4. Power on the system PC.
5. Power on all system instruments, except the process sample manager.
6. Log in to Windows.
7. Power on the process sample manager.
8. Log in to Empower software.
To power on the system after an emergency shutdown:

1. Power off each system instrument.
2. Reset the emergency-stop button by rotating it clockwise.
3. Set the master AC disconnect control to the “on” position.
4. Set the circuit breakers to the “on” position.
5. Power on the system PC.
6. Power on all system instruments, except the process sample manager.
7. Log in to Windows.
8. Power on the process sample manager.
9. Log in to Empower software.
Shutting down the system

To shut down the system:
1. Exit all software and power off the system PC.
2. Power off all system instruments.
3. Set the master AC disconnect control to the “off” position.
4. Set the circuit breakers to the “off” position.
Important: If you need to immediately remove AC power from the PATROL
UPLC Process Analyzer system, press the emergency-stop button located on
the front of the options compartment.
Maintenance considerations 4-7

Accessing or removing instrument components
Each of the instrument components rests on a sliding drawer. When the door
to the instrument compartment is fully open, the components can slide
forward, enabling access to panels and connections for maintenance or
removal.
Requirement: You must use the release levers on the right-hand and left-hand
sides of each drawer slide before pulling it forward (levers located near the
front) or moving it after it is fully extended (levers located near the back).
Drawer slide release mechanisms:
Front of
drawer slide
TP03345
Fully extended
drawer slide
The binary solvent manager, column manager, auxiliary column manager,

and detector are components of an ACQUITY UPLC system. Find operational
details for these instrument modules in the user documentation on the
PATROL documentation CD.

See also: ACQUITY UPLC user documentation on the PATROL
documentation CD for details on connecting and disconnecting ACQUITY
components.
Caution: To avoid damaging instruments, ensure that any tubing

connections between instrument components are removed before
sliding a drawer forward.
Warning: To avoid injury when you are removing an instrument:

• Ensure that its sliding drawer is pulled completely forward.
• Use an appropriate scissor lift table to support and transport the
instrument from the enclosure.
Disconnecting the process sample manager

The process sample manager is specific to the PATROL system. See the
instructions for managing connections to the process sample manager, below.
To disconnect the process sample manager:

1. Stop flow to the process sample manager and power-off the instrument.
2. Remove any external tubing connections to the process sample manager.
(See figure “Process sample manager’s flow path:” on page 1-10.)
Sliding an instrument forward or removing it from the enclosure

To slide an instrument forward or remove it from the enclosure:
1. Unlock the instrument compartment door, if necessary, and fully open
it.
2. If necessary for a maintenance procedure or removal, power-off the
instrument.
Caution: To avoid damaging electrical parts, never disconnect an

electrical assembly while power is applied to an instrument. To
completely interrupt power to an instrument, set the power
switch to Off, and then unplug the power cord from the rear of
the instrument. After power is removed, wait 10 seconds
thereafter before you disconnect an assembly.
Accessing or removing instrument components 4-9

3. Disconnect any tubing connections to the process sample manager (see
page 4-9).
Caution: To avoid damaging instruments, ensure that any tubing

connections between instrument components are removed before
sliding a drawer forward.
4. Press the release mechanism for the desired drawer, and slowly pull the
platform forward until it stops.
5. Remove the power and ethernet cables from the rear of the instrument.

switch to Off, and then unplug the power cord from the rear of
the instrument. After power is removed, wait 10 seconds
thereafter before you disconnect an assembly.
Rear-panel of the process sample manager:
Fuse holder
Ethernet connection
Power connection

6. Perform maintenance or transfer the instrument to an appropriate
scissor lift table for transport.
Warning: To avoid injury when you are removing an instrument:

• Ensure that its sliding drawer is pulled completely forward.
• Use an appropriate scissor lift table to support and transport the
instrument from the enclosure.
Adding or replacing solvents

The solvent supply for chromatographic analysis is inside the solvent/waste
compartment, located at the bottom, right-hand side of the system enclosure.
The solvent reservoirs occupy a secondary containment receptacle on the
upper-most sliding drawer of the compartment.
To add or replace solvents:

1. Unlock the solvent/waste compartment, if necessary, and fully open it.
2. Press the release mechanism for the desired drawer, and slowly pull the
platform forward until it stops.
3. Identify the solvent line and reservoir you are servicing, and secure the
end of the tubing to prevent spills.
Warning: To prevent contact with hazardous chemicals, wear

clean, chemical-resistant, powder-free gloves when working with
solvents.
4. Replace the reservoir, or add solvent, as needed.

5. Slide the drawer back into place, and close the front-panel door of the
compartment.
Emptying waste containers

Solvent waste drains to the same compartment that houses the analysis
solvents, filling two waste containers.
Adding or replacing solvents 4-11

To empty solvent waste:
Warning: To prevent contact with hazardous chemicals, wear

clean, chemical-resistant, powder-free gloves when working with
solvents.
1. Open the solvent/waste compartment door, and pull out the waste
drawer.
Tip: See “Adding or replacing solvents” on page 4-11 for information
about how to access the compartment and drawers.
2. Identify which waste container you are emptying.
3. Secure the end of the waste tubing to prevent spills.
4. Remove the waste container, and transfer the contents to an appropriate
disposal location.
5. Replace the waste container and tubing line.
6. Slide the drawer back into place, and close the front-panel door.
Switching columns
Access to the column manager to add/remove analysis columns can be
restricted to specific user types. Authorized users can find information on
performing these tasks in the column compartments user documentation,
provided on the PATROL documentation CD.
Relocating the system


system enclosure with instruments or reservoirs installed. (See
“Accessing or removing instrument components” on page 4-8.)
Maintaining the process sample manager

Valve control, process pump control, diagnostic tests and maintenance
procedures for the process sample manager are accessible through the
ACQUITY Console.
Important:
• Accessing the window for a specific test or procedure moves the
instrument assemblies to the correct starting position for that task.
• When you exit a diagnostic or maintenance window, the process sample
manager performs a reset.
Tip: The control, test, and maintenance windows are available in Empower
software and from the PATROL touch-screen interface.
Online valve control

Online Valve Control window:

When you access the Online Valve Control window, the sample needle moves
to the injection port position. From this page, you can select process valve and
sample valve positions and control process pump flow. A pressure value,
displayed in the window, helps determine whether all lines are flowing
properly.
Tip: The window indicates status in addition to allowing you to control valves
and pumps. The selected option displayed in the window is the current
position of the valve or pump.
To access the Online Valve Control window:

1. In the ACQUITY UPLC Console software, select Process Sample
Manager from the system tree.
2. In the process sample manager information window, click Maintain >
Online Valve Control.
Priming the online flow path

Online Prime window:
When you access the priming window, the sample needle moves to the
injection port position.
From the priming window, you can use the process pump to prime the process
valve and each of the six process lines.

To access the priming window:
2. In the process sample manager information window, click Control >
Online Prime.
Note: To prime the atline flow path, click Control > Prime.
Running a leak test

Leak Test window:
You can select which section of the flow path to test for leaks:
• Sample – tests for leaks along the flow path from the sample pump to
the priming valve (the priming valve is blocked).
• Diluent – tests for leaks along the flow path from the diluent pump to
the priming valve (the priming valve is blocked).
• System – tests for leaks along the flow paths from both pumps.through
the injection port. The priming valve and the injection valve are
blocked.and the needle is in the injection port during the test. The leak
test includes the needle seal.

When you start a leak test from this window, the description of each test step
is highlighted as it is executed.
To access the leak test window:

Leak Test.
Calibrating the needle seal

To calibrate the needle seal:
Calibrate Seal.
3. In the window that appears, use the controls to calibrate the needle seal.
Seal calibration window:
When you access the seal calibration window and start a calibration,
each step is highlighted as it is executed.

Upgrading firmware
Upgrade firmware window:
You can upgrade the process sample manager’s firmware from this window.
To access the Upgrade firmware window:

Upgrade Firmware.
Note: If the instrument control software (ICS) is installed, you can
browse to the appropriate firmware file on your computer’s local drive
(C:\Empower\Instruments\Firmware\ACQUITY Process Sample
Manager.sft, for example) and start the upgrade. Consult the software
documentation for details.
Resolving leak sensor errors

After approximately 1.5 mL of liquid accumulate in the leak sensor reservoir,
an alarm sounds, indicating that the leak sensor detected a leak.
Warning: The leak sensor can be contaminated with toxic

materials. Always wear clean, chemical-resistant, powder-free
gloves when performing this procedure.

Caution: To avoid scratching or damaging the leak sensor
• do not allow buffered solvents to accumulate and dry on it.
• do not submerge it in a cleaning bath.
Required materials
• Clean, chemical-resistant, powder-free gloves
• Cotton swabs
• Nonabrasive, lint-free wipes
®
• T10 TORX screwdriver
• 4-mm hex wrench
To resolve a process sample manager leak sensor error:

1. View the Leak Sensors dialog box in the ACQUITY UPLC Console to
confirm that the process sample manager’s leak sensor detected a leak.
If an error message appears that reads “Leak Detected”, power-off the
process sample manager.

switch to Off, and then unplug the power cord from the AC
outlet. After power is removed, wait 10 seconds thereafter before
you disconnect an assembly.
2. Using the hex wrench, remove the two screws securing the bottom-front
panel to the process sample manager.
3. Remove the bottom panel from the front of the process sample manager
by gently pulling both the right-hand and left-hand edges toward you.

Front of the process sample manager:
Top panel
Bottom panel
Leak sensor reservoir
4. Locate the source of the leak, and make the repairs necessary to stop the
leak.
Caution: To avoid damaging the leak sensor, do not grasp it by

the ribbon cable.

5. Using the TORX screwdriver, remove the two screws securing the leak
sensor reservoir to the process sample manager.
Screw (right-hand side)
Screw (left-hand side)
6. Lower the reservoir until the top of the leak sensor is accessible.

7. Remove the leak sensor from its reservoir by grasping it by its serrations
and pulling upward on it.
Leak sensor
8. Tip: If you cannot easily manipulate the leak sensor after removing it
from its reservoir, detach the connector from the front of the instrument.

the ribbon cable.
Connector
Ribbon cable

9. Use a nonabrasive, lint-free wipe to dry the leak sensor prism.
Prism
Lint-free wipe
TP02891
10. Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from
the leak sensor reservoir and its surrounding area.
11. With a cotton swab, absorb any remaining liquid from the corners of the
leak sensor reservoir and its surrounding area.
12. Align the leak sensor’s T-bar with the slot in the side of the leak sensor
reservoir, and slide the leak sensor into place.
TP02892
T-bar TP03343
Slot in leak
sensor reservoir
13. If you detached the connector from the front of the instrument, reattach
it.
14. In the ACQUITY UPLC Console, select Process Sample Manager from
the system tree.
Reset PSM, to reset the process sample manager.

Replacing the process sample manager’s leak sensor
Warning: The leak sensor can be contaminated with toxic
materials. Always wear clean, chemical-resistant, powder-free
gloves when performing this procedure.
Required materials
• Clean, chemical-resistant, powder-free gloves
• New leak sensor
• 4-mm hex wrench
To replace the process sample manager’s leak sensor:

1. Using the hex wrench, remove the two screws securing the bottom-front
panel to the process sample manager.
2. Remove the bottom panel from the front of the process sample manager
by gently pulling both the right-hand and left-hand edges toward you.
3. Using the TORX screwdriver, remove the two screws securing the leak
sensor reservoir to the process sample manager.
Screw (right-hand side)

4. Lower the reservoir until the top of the leak sensor is accessible.
5. Press the tab to detach the leak sensor connector from the front of the
instrument.

the ribbon cable.
Connector
Ribbon cable

6. Remove the leak sensor from its reservoir by grasping it by its serrations
and pulling upward on it.
Leak sensor
7. Unpack the new leak sensor.

8. Align the leak sensor’s T-bar with the slot in the side of the leak sensor
reservoir, and slide the leak sensor into place.
TP02892
T-bar TP03343
Slot in leak
sensor reservoir
9. Plug the leak sensor connector into the front of the instrument.
10. Reattach the leak sensor reservoir to the process sample manager.
11. In the ACQUITY UPLC Console, select Process Sample Manager from
the system tree.
Reset PSM, to reset the process sample manager.

Replacing the sample loop
Warning: To prevent injury, always observe Good Laboratory
Practices when you handle solvents, change tubing, or operate the
process sample manager. Know the physical and chemical properties
of the solvents you use. See the Material Safety Data Sheets for the
solvents in use.
Caution: To prevent contamination, wear clean, chemical-resistant,

powder-free gloves when replacing the sample loop.
Caution:
• To prevent bandspreading or excessive carryover, be sure to
reinstall a sample loop in exactly the same way that it was
previously installed, so that the same end goes into the same port.
• To avoid leaks, remove the O-rings prior to installing the sample
loop. The O-rings on the new sample loop hold the fittings in place
during shipping.
Replace the sample loop when it clogs or when its capacity is inappropriate for
your chromatographic need.
Required materials
• 1/4-inch open-end wrench
• ACQUITY UPLC sample loop
To replace the sample loop:

1. Stop flow to the process sample manager.
2. Remove the top panel from the front of the process sample manager.

3. Use the wrench to remove the fittings on ports 1 and 4 of the injection
valve.
Fittings on the injection valve:
To waste Sample loop
From mixer To column

manager
Sample loop From binary

solvent
manager
TP03023
4. Remove the sample loop and its fittings from the valve.
5. If you are going to reuse the sample loop, label each end with the port it
was connected to.
Tip: If you replaced the injection valve cartridge, do not install
previously used sample loops on the new cartridge.
6. Place the sample loop and its fittings in a contamination-free bag.

7. Unpack the replacement sample loop and fittings.
Sample loop (assembled):

To ports 1 and 4 on
injection valve
Caution: To avoid leaks, remove the O-rings prior to installing

the sample loop. The O-rings on the new sample loop are used to
hold the fittings in place during shipping.
8. Remove the O-rings from the sample loop.
Caution:
• To prevent dead volume in the system, ensure that the ends of
the loop are fully inserted into the ports of the injection valve
before swaging the ferrules.
• Use only Waters approved ACQUITY UPLC loops with
ACQUITY UPLC fittings. Each loop must be fitted to its
injector.
9. Ensure the 2-piece ferrule is in place on one end of the sample loop. If
not, slide a 2-piece ferrule and compression screw onto one end of the
sample loop, and then seat the end into injection valve port 1.
10. Finger-tighten the fitting and then add 3/4-turn with the wrench.
11. Remove the fitting and inspect the ferrule to ensure it has not moved.

Ferrule installation:
Ferrule Tubing Ferrule Tubing
TP02506 TP02507
Correct Incorrect
12. Repeat step 8 through step 10 for the other end of the sample loop and
for injection valve port 4.
13. Reinstall the sample loop fitting into valve ports 1 and 4.
Caution: To prevent bandspreading or excessive carryover, be

sure to reinstall the sample loop in exactly the same way that it
was previously installed, so that the same end goes into the same
port.
14. Replace the top panel.

16. In the process sample manager information window, click Configure >
Volumes, and then specify the volume of the new sample loop.

Replacing pumps and valves
Warning: To prevent injury, always observe Good Laboratory
Practices when you handle solvents, change tubing, or operate the
process sample manager. Know the physical and chemical properties
of the solvents you use. See the Material Safety Data Sheets for the
solvents in use.
Caution: To prevent contamination, wear clean, chemical-resistant,

powder-free gloves when replacing the injection valve cartridge.
Valves and pumps in the process sample manager:
Process pump
Sampling
valve
Sample
Injection
pump
valve
Diluent
pump Process
valve
Priming
valve
Mixer
To prepare a pump or valve for replacement:

Replace Pumps/Valves.
3. In the window that appears, use the controls to prepare the pump or
valve for replacement.

Replace Pumps/Valves window:
Required materials
• 2-mm hex wrench
• 1/4-inch open-end wrench
• Pump or valve cartridge
• Sample loop, if necessary
To replace a valve cartridge:

Requirement: When you replace the injection valve cartridge, you must
also replace the sample loop.

1. Prepare the valve for replacement (see “To prepare a pump or valve for
replacement:” on page 4-30).
2. Verify that there is no flow to the process sample manager and power-off
the instrument.
3. Remove the top panel from the front of the process sample manager.
4. Remove the fittings attached to the valve cartridge.
Fittings on the injection valve:
Set screw
To waste Sample loop
From mixer To column

manager
Sample loop From binary

solvent
manager
TP03023

Fittings on the process valve:
Set screw
Process line #1
Process line #2
Process line #3 Process line #6
To process
pump
Process line #4 Process line #5
Fittings on the sampling valve:
Set screw
Plugged Plugged
Connected to To waste
port 1 of priming
valve
Needle Sensor
(from
process
pump)
TP03023

Fittings on the priming valve:
Set screw
Connected to port 3
Diluent of sampling valve
Transducer Transducer
(diluent pump) (sample pump)
Injection port Wash
5. Use the 2-mm hex wrench to remove the set screw at the 10 o’clock
position on the valve cartridge.
6. Remove the valve cartridge from the valve assembly by pulling it toward
you.
7. Carefully unpack the replacement valve cartridge.
8. Ensure that the groove in the cartridge housing and the groove on the
drive clamp are aligned. If they are not, turn the drive clamp until the
grooves line up.

Housing for injection and sampling valves:
Valve cartridge housing
Aligned grooves
Drive clamp
9. Insert the new valve cartridge into the valve assembly.

Requirement: If the valve cartridge does not slide fully into the valve
assembly, contact your Waters service representative.
10. Insert the 2-mm set screw at the 10 o’clock position on the valve
cartridge, and use the 2-mm hex wrench to tighten it.
11. Reattach all fittings and install a new sample loop, if necessary (see
page 4-26).
Tip: To prevent bandspreading and excessive carryover, do not install
previously used sample loops on the new cartridge.
12. Push the valve back to the closed position.
13. Replace the top panel.
14. Power-on the process sample manager.
Prime.
17. In the Prime dialog box
a. specify 20 seconds for the external needle wash.
b. specify 5 cycles for the sample wash.
c. specify 5 cycles for the diluent.

d. specify 0 seconds for the flush time.
Note: You do not need to specify a process line.
e. click OK. The process sample manager status displays “Priming”.
When priming is complete, the status returns to “Idle”.
Required materials
• 3/32-inch hex wrench
®
• Phillips screwdriver
• Replacement sample pump
To replace a sample pump:

1. Prepare the sample pump for replacement (see “To prepare a pump or
valve for replacement:” on page 4-30).
the instrument.
3. Remove the top panel from the front of the process sample manager by
grasping both the right-hand and left-hand sides and pulling toward
you.
4. Remove the middle panel from the top of the process sample manager by
you.

5. Remove the finger-tight fitting connecting the sample pump to the
transducer.
Sample pump fitting
Sample pump
Transducer
6. Remove the three electrical connectors from the top of the sample pump.
Important: Use the Phillips screwdriver to remove the two screws
securing the sensor connector to the pump.

7. Using the hex wrench, remove the two screws (one on each side)
securing the pump to the mounting bracket.
Motor
connection Sensor connection
Encoder
connection Fitting
Sample pump
Diluent pump
Mounting bracket
8. Grasp the front of the pump and remove it from the instrument.
9. Unpack the new pump, if necessary.
10. Position the pump on the mounting bracket
11. Using the hex wrench and mounting screws, secure the replacement
pump to the bracket.
12. Attach the sensor, motor, and encoder connectors to the appropriate
locations.
13. Replace the process sample manager’s top and front panels.
Required materials
• 3/32-inch hex wrench
• Phillips screwdriver
• Replacement diluent pump

To replace a diluent pump:
1. Prepare the diluent pump for replacement (see “To prepare a pump or
the instrument.
you.
you.
5. Remove the fitting connecting the diluent pump to the transducer.
Diluent pump fitting
Diluent pump
Transducer

6. Using the TORX screwdriver, remove the two screws (one on each side)
securing the pump mounting bracket to the instrument’s support
platform.
Sample pump
Mounting bracket
Diluent pump
7. Grasp the entire pump assembly (including mounting bracket and

pumps, if present) and tilt it at an angle to access the electrical
connectors beneath the diluent pump.
8. Remove the three electrical connectors from the bottom of the diluent
pump. Important: Use the Phillips screwdriver to remove the two screws
securing the sensor connector to the pump.
9. Lower the pump assembly to its original position.
10. Using the hex wrench, remove the two screws (one on each side)
securing the pump to the mounting bracket.

Diluent pump
Mounting bracket
11. Grasp the front of the pump and remove it from the instrument.

13. Position the pump beneath the mounting bracket
14. Using the hex wrench and mounting screws, secure the replacement
pump to the bracket.
15. Grasp the entire pump assembly (including mounting bracket and
pumps, if present) and tilt it at an angle to replace the electrical
connectors to the bottom of the diluent pump.
16. Attach the sensor, motor, and encoder connectors to the appropriate
locations on the pump.
17. Replace the mounting screws securing the mounting bracket to the
instrument’s support platform.
18. Replace the process sample manager’s top and front panels.
Required materials
To replace a process pump and install tubing:

1. Prepare the process pump for replacement (see “To prepare a pump or
the instrument.
you.
you.

5. Open the cover of the process pump and remove the tubing.
Process pump cover
Process pump tubing
6. Remove the two electrical connectors from the back of the process pump.
Front of process pump
Process pump mounting bracket
Electrical connections
Mounting screws
7. Using the TORX wrench, remove the two mounting screws securing the
process pump assembly to the instrument’s support platform.
8. Remove the pump assembly from the instrument.

10. Using the TORX wrench and mounting screws, secure the replacement
pump to the instrument’s support platform.
11. Reattach the two electrical connections to the back of the pump.
12. Reinstall the process pump tubing.
Caution: To avoid damaging the process tubing, ensure that it is

routed through the two tubing guides and kept under slight
tension while you close the pump cover.
Tip: The process pump tubing is connected to the flow path by two
finger-tight fittings. The fittings are pre-installed on new tubing
assemblies.
Process pump tubing
Finger-tight fittings
Replacing the mixer

Required materials
• Replacement mixer
To replace the mixer:

1. Gently pull the mixer upwards out of the holding clip.
2. Remove the finger-tight fitting on each end.
3. Attach the fittings to the end of the new mixer.
4. Secure the new mixer in the holding clip.

Cleaning the filters
Required materials
• 2.5-mm hex wrench
• Clean water
To remove and clean the filters:

1. Using the hex wrench, remove the top and bottom screws from the filter
panels on the left-hand and right-hand sides of the process sample
manager.
Screw
Front of process
sample manager
Filter panel
Screw

2. Remove the metal supports from the back of the filter panels by sliding
them first to one side and then the other, lifting the metal tabs securing
the support to the assembly as they become visible.
Metal support
Metal tab
3. Remove the soft filter material from the filter assemblies.

4. Rinse the soft filters thoroughly in clean water.
5. To dry the filters, absorb excess water with lint-free wipes and then
allow the filter to air dry.
6. When they are completely dry, reassemble the filter panels.
Tip: To replace the metal support, insert the straight edge first, and then
slide it to each side to secure the end tabs.
Replacing the fuses

See also: Information about fuses in other ACQUITY modules is located at
the beginning of the fuse maintenance procedures for each module. Consult
the PATROL documentation CD for related maintenance information.
Warning: To avoid electric shock, power-off and unplug the process

sample manager before examining fuses. For continued protection
against fire, replace fuses with those of the same type and rating
only.
Suspect an open or otherwise defective fuse when any of these conditions

applies:
• The process sample manager fails to power-on.

• The process sample manager status LEDs are unlit.
• The power supply fan does not operate.
Required materials
• Fuses (see Appendix C for type)
• Flat-blade screwdriver
To replace the fuses:

Requirement: Replace both fuses, even when only one is open or defective.

1. Power-off the process sample manager.

Tip: The fans inside the process sample manager run continuously, even
after you power it off.
2. Disconnect the power cord from the power entry module.
3. Use a flat-blade screwdriver to open the fuse holder door, located above
the power entry module on the rear panel.
4. With minimum pressure, pull on each spring-loaded fuse holder and
remove it.
5. Remove and discard the fuses.
Warning: For continued protection against fire, replace fuses

with the appropriate type and rating.
6. Insert the new fuses into the holders and the holders into the power
entry module.
7. Reconnect the power cord to the power entry module.

Cleaning the system enclosure’s exterior
Use a soft cloth, dampened with water, to clean the outside of the system
enclosure.
Cleaning the system enclosure’s exterior 4-47

A Safety Advisories
Waters instruments display hazard symbols designed to alert you to the

hidden dangers of operating and maintaining the instruments. Their
corresponding user guides also include the hazard symbols, with
accompanying text statements describing the hazards and telling you
how to avoid them. This appendix presents all the safety symbols and
statements that apply to the entire line of Waters products.
Note: Not all of the warnings and advisories listed in Appendix A are
applicable to the PATROL system.
Contents:
Topic Page
Warning symbols A-2
Caution symbol A-4
Warnings that apply to all Waters instruments A-4
Electrical and handling symbols A-11
A-1
Warning symbols
Warning symbols alert you to the risk of death, injury, or seriously adverse
physiological reactions associated with an instrument’s use or misuse. Heed
all warnings when you install, repair, and operate Waters instruments.
Waters assumes no liability for the failure of those who install, repair, or
operate its instruments to comply with any safety precaution.
Task-specific hazard warnings

The following warning symbols alert you to risks that can arise when you
operate or maintain an instrument or instrument component. Such risks
include burn injuries, electric shocks, ultraviolet radiation exposures, and
others.
When the following symbols appear in a manual’s narratives or procedures,
their accompanying text identifies the specific risk and explains how to avoid
it.
Warning: (General risk of danger. When this symbol appears on an

instrument, consult the instrument’s user documentation for important
safety-related information before you use the instrument.)
Warning: (Risk of burn injury from contacting hot surfaces.)
Warning: (Risk of electric shock.)
Warning: (Risk of fire.)
Warning: (Risk of needle puncture.)
Warning: (Risk of injury caused by moving machinery.)
Warning: (Risk of exposure to ultraviolet radiation.)
Warning: (Risk of contacting corrosive substances.)
Warning: (Risk of exposure to a toxic substance.)
Warning: (Risk of personal exposure to laser radiation.)
A-2 Safety Advisories

Warning: (Risk of exposure to biological agents that can pose a serious
health threat.)
Warning: (Risk of eye injury.)
Specific warnings
The following warnings can appear in the user manuals of particular
instruments and on labels affixed to them or their component parts.
Burst warning
This warning applies to Waters instruments fitted with nonmetallic tubing.
Warning: Pressurized nonmetallic, or polymer, tubing can burst.

Observe these precautions when working around such tubing:
• Wear eye protection.
• Extinguish all nearby flames.
• Do not use tubing that is, or has been, stressed or kinked.
• Do not expose nonmetallic tubing to incompatible compounds like
tetrahydrofuran (THF) and nitric or sulfuric acids.
• Be aware that some compounds, like methylene chloride and
dimethyl sulfoxide, can cause nonmetallic tubing to swell, which
significantly reduces the pressure at which the tubing can rupture.
Chemical hazard warning

This warning applies to Waters instruments that can process corrosive, toxic,
flammable, or other types of hazardous material.
Warning: Waters instruments can be used to analyze or

process potentially hazardous substances. To avoid injury
with any of these materials, familiarize yourself with the
materials and their hazards, observe Good Laboratory
Practices (GLP), and consult your organization’s safety
representative regarding proper use and handling.
Guidelines are provided in the latest edition of the National
Research Council's publication, Prudent Practices in the
Laboratory: Handling and Disposal of Chemicals.
Warning symbols A-3

Caution symbol
The caution symbol signifies that an instrument’s use or misuse can damage
the instrument or compromise a sample’s integrity. The following symbol and
its associated statement are typical of the kind that alert you to the risk of
damaging the instrument or sample.
Caution: To avoid damage, do not use abrasives or solvents to clean the

instrument’s case.
Warnings that apply to all Waters instruments

When operating this device, follow standard quality control procedures and
the equipment guidelines in this section.

Attention: Changes or modifications to this unit not expressly approved by the
party responsible for compliance could void the user’s authority to operate the
equipment.
Important: Toute modification sur cette unité n’ayant pas été expressément
approuvée par l’autorité responsable de la conformité à la réglementation peut
annuler le droit de l’utilisateur à exploiter l’équipement.
Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die

ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit
verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des
Systems führen.
Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non

espressamente autorizzata dai responsabili per la conformità fa decadere il
diritto all'utilizzo dell'apparecchiatura da parte dell'utente.
Atencion: cualquier cambio o modificación efectuado en esta unidad que no

haya sido expresamente aprobado por la parte responsable del cumplimiento
puede anular la autorización del usuario para utilizar el equipo.
注意：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設
備的權利。
注意：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操
作该设备的合法性。
주의: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우,

이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.
注意：規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユー
ザーとしての承認が無効になる可能性があります。

Warning: Use caution when working with any polymer tubing under pressure:
• Always wear eye protection when near pressurized polymer tubing.
• Extinguish all nearby flames.
• Do not use tubing that has been severely stressed or kinked.
• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated
nitric or sulfuric acids.
• Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic
tubing to swell, which greatly reduces the rupture pressure of the tubing.
Attention: Manipulez les tubes en polymère sous pression avec precaution:
• Portez systématiquement des lunettes de protection lorsque vous vous
trouvez à proximité de tubes en polymère pressurisés.
• Eteignez toute flamme se trouvant à proximité de l’instrument.
• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.
• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF)
ou de l'acide sulfurique ou nitrique concentré.
• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le
gonflement des tuyaux non métalliques, ce qui réduit considérablement leur
pression de rupture.
Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere
Vorsicht angebracht:
• In der Nähe von unter Druck stehenden Polymerschläuchen stets
Schutzbrille tragen.
• Alle offenen Flammen in der Nähe löschen.
• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind.
• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder
konzentrierte Salpeter- oder Schwefelsäure verwenden.
• Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische
Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich
reduziert.

Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico
sotto pressione:
• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero
pressurizzati.
• Spegnere tutte le fiamme vive nell'ambiente circostante.
• Non utilizzare tubi eccessivamente logorati o piegati.
• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico
o nitrico concentrati.
• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano
rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di
rottura dei tubi stessi.
Advertencia: se recomienda precaución cuando se trabaje con tubos de
polímero sometidos a presión:
• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos
de polímero sometidos a presión.
• Si hubiera alguna llama las proximidades.
• No se debe trabajar con tubos que se hayan doblado o sometido a altas
presiones.
• Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano
(THF) o ácidos nítrico o sulfúrico concentrados.
• Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo
dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los
tubos.
警告：當在有壓力的情況下使用聚合物管線時，小心注意以下幾點。
• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。
• 熄滅附近所有的火焰。
• 不要使用已經被壓癟或嚴重彎曲管線。
• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。
• 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹，大大降低管線的耐壓能力。

警告：当有压力的情况下使用管线时，小心注意以下几点：
• 当接近有压力的聚合物管线时一定要戴防护眼镜。
• 熄灭附近所有的火焰。
• 不要使用已经被压瘪或严重弯曲的管线。
• 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。
• 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。
경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.

• 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.
• 근처의 화기를 모두 끄십시오.
• 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.
• 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는
농축 질산 또는 황산과 함께 사용하지 마십시오.
• 염화 메틸렌(Methylene chloride) 및 디메틸술폭시드(Dimethyl sulfoxide)는
비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오.
警告：圧力のかかったポリマーチューブを扱うときは、注意してください。
• 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。
• 近くにある火を消してください。
• 著しく変形した、または折れ曲がったチューブは使用しないでください。
• 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流
さないでください。
• 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合が
あり、その場合、チューブは極めて低い圧力で破裂します。

Warning: The user shall be made aware that if the equipment is used in a
manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une
façon non spécifiée par le fabricant, la protection assurée par le matériel risque
d’être défectueuses.
Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei

unsachgemäßer Verwenddung des Gerätes die eingebauten
Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß
funktionieren.
Attenzione: si rende noto all'utente che l'eventuale utilizzo

dell'apparecchiatura secondo modalità non previste dal produttore può
compromettere la protezione offerta dall'apparecchiatura.
Advertencia: el usuario deberá saber que si el equipo se utiliza de forma

distinta a la especificada por el fabricante, las medidas de protección del equipo
podrían ser insuficientes.
警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供
的保護將被消弱。
警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供
的保护将被削弱。
경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이

제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다.
警告：ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供
している保証が無効になる可能性があることに注意して下さい。

Warning: To protect against fire, replace fuses with those of the type
and rating printed on panels adjacent to instrument fuse covers.
Attention: pour éviter tout risque d'incendie, remplacez toujours les
fusibles par d'autres du type et de la puissance indiqués sur le panneau
à proximité du couvercle de la boite à fusible de l'instrument.
Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit
Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben
den Sicherungsabdeckungen des Geräts gedruckt sind.
Attenzione: per garantire protezione contro gli incendi, sostituire i
fusibili con altri dello stesso tipo aventi le caratteristiche indicate sui
pannelli adiacenti alla copertura fusibili dello strumento.
Advertencia: Para evitar incendios, sustituir los fusibles por aquellos
del tipo y características impresos en los paneles adyacentes a las
cubiertas de los fusibles del instrumento.
警告 : 為了避免火災，更換保險絲時，請使用與儀器保險絲蓋旁面板上所印刷之相同類
型與規格的保險絲。
警告 : 为了避免火灾，应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的
保险丝。
경고: 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한

타입 및 정격의 제품으로 퓨즈를 교체하십시오.
警告 : 火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記
載されているタイプおよび定格のヒューズをご使用ください。

Electrical and handling symbols
Electrical symbols
These can appear in instrument user manuals and on the instrument’s front
or rear panels.
Electrical power on
Electrical power off
Standby
Direct current
Alternating current
Protective conductor terminal
Frame, or chassis, terminal
Fuse
Recycle symbol: Do not dispose in municipal waste.
Electrical and handling symbols A-11

Handling symbols
These handling symbols and their associated text can appear on labels affixed
to the outer packaging of Waters instrument and component shipments.
Keep upright!
Keep dry!
Fragile!
Use no hooks!

B Materials of Construction and
Compatible Solvents
Warning: To confirm the integrity of the source exhaust

system, you must address any safety issues raised by the
contents of this Appendix.
See also: For additional information on compatible solvents (except

tetrahydrofuran and hexane), see Appendix C of the ACQUITY UPLC
System Operator’s Guide. Important information about avoiding the use
of tetrahydrofuran and hexane on the PATROL system appears below.
Contents
Topic Page
Preventing contamination B-2
Items exposed to solvent B-2
Solvents used to prepare mobile phases B-2
B-1
Preventing contamination
For information on preventing contamination, visit www.waters.com and refer
to Controlling Contamination in LC/MS Systems (part number 715001307).
Items exposed to solvent

The items that appear in the following table can be exposed to solvent. You
must evaluate the resultant safety issues if the solvents used in your
application differ from those normally used with these items. See “Solvents
used to prepare mobile phases” on page B-2 for details about the most common
ingredients used to prepare mobile phases.
Materials of construction:
Item Material
Wetted materials 316 SST, UHMWPE, synthetic
sapphire, synthetic ruby, FEP,
PTFE, ETFE, diamond-like coating,
PEEK, PEEK alloys, titanium alloys
Process sample manager, chassis 5051-H32 Aluminum
Process sample manager, external 5051-H32 Aluminum
panels
Process sample manager, front 5051-H32 Aluminum
panel
Enclosure panels 304 SST, 11 ga and 13 ga
Enclosure finish Number 4 finish
Drip trays Polyethylene
Solvents used to prepare mobile phases

Following are the most common solvents, additives, and modifiers used to
prepare mobile phases for use on the PATROL system:
• Water
• Methanol
• Acetonitrile
B-2 Materials of Construction and Compatible Solvents

• Isopropanol
• Urea (7 M)
• Formic acid (0.2%)
• Trifluoroacetic acid (0.1%)
• Triethylamine (0.1%)
• Ethylenediaminetetraacetic acid (0.1%)
• Phosphate buffer (10 mM)
• Ammonium bicarbonate (10 mM)
• Ammonium hydroxide (50 mM)
• Ammonium acetate (50 mM)
These solvents are not expected to cause any problems when used in
combination with the materials identified in “Items exposed to solvent” on
page B-2.
The following solvents are NOT compatible with the PATROL system:
Caution: To avoid damage to the system, do not use the solvents listed
below on the PATROL system.
• Toluene
• Methylene chloride
• Trichlorobenzene
• Ethyl acetate
• Chloroform
• Chlorinated solvents
• Strong acids (greater than 5% v/v)
• Hexane
• Tetrahydrofuran
Important: Tetrahydrofuran and hexane are not recommended for use on the
PATROL system. This restriction differs from solvent recommendations of
some other ACQUITY UPLC systems.
Solvents used to prepare mobile phases B-3

B-4 Materials of Construction and Compatible Solvents
C Specifications
See also: For the specifications of the binary solvent manager, column
manager, and auxiliary column manager, and detectors, consult the
appropriate user documentation on the PATROL documentation CD.
Contents
Topic Page
Specifications for the PATROL UPLC Process Analyzer C-2
Specifications for the process sample manager C-3
C-1
Specifications for the PATROL UPLC Process Analyzer
PATROL UPLC Process Analyzer physical specifications:
Attribute Specification
Height 72.0 inches (77.0 inches with light tower installed)
Width 30.0 inches
Depth 33.0 inches
Weight 1,019 pounds
Rear clearance 0.5 inch, minimum clearance required
Side clearance 0.5 inch, minimum clearance required
PATROL UPLC Process Analyzer electrical specifications:
Moisture protection
a Normal (IPX0)
Voltage range 100 to 240 VAC
Frequency 50/60 Hz
Power consumption 1000 VA nominally
a. Moisture Protection – Normal (IPX0) – IPX0 means that no Ingress Protection against any
type of dripping or sprayed water exists. The X is a placeholder that identifies protection
against dust, if applicable.
PATROL UPLC Process Analyzer environmental specifications:
Operating temperature 4 to 35 °C (39.2 to 95 °F)
Operating humidity 20% to 80%, noncondensing
Shipping and storage temperature -40 to 60 °C (-22 to 140 °F)
Shipping and storage humidity 10% to 90%, noncondensing
See also: For the performance specifications of the PATROL UPLC Process
Analyzer System, consult the information for the individual instrument
C-2 Specifications
components, available in the user documentation on the PATROL
documentation CD.
Specifications for the process sample manager
Process sample manager physical specifications:
Height 21.5 inches
Width 13.5 inches
Depth 26 inches
Weight 110 pounds
Rear clearance 2 inches, required minimum clearance
Side clearance 1 inch, required minimum clearance
Process sample manager electrical specifications:
Pollution degree
a 2
Moisture protection
b Normal (IPX0)
Line voltages, nominal Grounded AC

Voltage range 100 to 240 VAC nominal
Frequency 50/60 Hz
Fuse Two fuses, F 6.3 A, 250 V (slow-blow),
5 × 20 mm (IEC)
Power consumption 450 VA nominal
Laser (internal to OEM bar-code Class 1
reader)
a. Pollution Degree 2 – A measure of pollution on electrical circuits, which can produce a
reduction of dielectric strength or surface resistivity. Degree 2 refers only to normally noncon-
ductive pollution. Occasionally, however, expect a temporary conductivity caused by conden-
sation.

b. Moisture Protection – Normal (IPX0) – IPX0 means that no Ingress Protection against any
type of dripping or sprayed water exists. The X is a placeholder that identifies protection
against dust, if applicable.
Process sample manager environmental specifications:
Operating temperature 4 to 40 °C (39.2 to 104 °F)
Operating humidity 20% to 80%, noncondensing
Shipping and storage temperature -30 to 60 °C (-22 to 140 °F)
Shipping and storage humidity 10% to 90%, noncondensing
Process sample manager performance specifications:
Maximum sample capacity 32 available sites for process and
control samples
Number of sample injections Up to 60 injections per sample vial
Sample Access Total random access
Injection modes Full-loop injections only. Injected
material can be from a vial or process
source.
Injection loop volumes 1-μL, 2-μL, or 5-μL sample loops
Dilution range Up to 1:50 in 0.1 increments
Injection precision (without dilution) Less than 0.5% RSD for caffeine, full
loop injection
Injection precision with dilution Less than 1.0% RSD for caffeine, full
loop injection
Injection linearity Greater than 0.999 coefficient of
deviation
1 to 50 dilution factor
Wash solvent consumption Variable (configured by user)
Sample carryover Less than 0.01% of the previous
injection for caffeine
C-4 Specifications
Process sample manager performance specifications: (Continued)
Diluent solvent consumption Variable (configured by user).
Minimum of 500 μL per injection for
wash.
Minimum injection cycle time 120 seconds (vial), or 180 seconds
(process line)
Minimum sample volume required 1,200 µL must be dispensed into the
vial in order to perform an injection.
Maximum sample consumed per 100 μL
analysis (vial)
Sample compartment temperature 4 to 40 ºC programmable in 0.1 ºC
control increments
Sample compartment temperature ± 0.5 ºC from set point measured at
stability the control sensor
Sample compartment temp. ± 1.0 ºC from set point measured at
accuracy the control sensor
Sample compartment temp. ± 3 ºC from set point as measured in
distribution air, not in a vial
Sample compartment cooling time Less than 60 minutes from 25 ºC to
4 ºC
Audible noise Less than 65 dBA

C-6 Specifications
Index
A configuration table 2-3
accessing instrument components 4-8 connections
ACQUITY UPLC system 1-8, 4-8 top of enclosure 1-4
advanced access to software tasks 1-12 construction materials B-1
atline decision-maker file 2-3 contamination, preventing B-2
audience and purpose vii counters, maintenance 4-3
auxiliary column manager 1-9, 4-2, 4-8
D
B decision-maker file 2-2
bar code 3-2, 3-4 atline 2-3
reading 1-9 online 2-4
sample ID 2-3 detectors iv
bar-code reader C-3 diagnostic tests 4-13
basic operator interface 1-12 diluent pump 4-39
binary solvent manager 4-2, 4-8 replacing 4-39
burst warning A-3 dilutions 1-9, 2-3
disconnecting the process sample
C manager 4-9
calibrating disposal of Waters products iii, vi
needle seal 4-16 documentation, ACQUITY UPLC 1-8
carousel 1-11 drawer 1-7, 4-8
occupancy 3-2 release 4-8
viewing 3-5
cartridge E
valve, replacing 4-31 EC Authorized Representative ix
caution symbol A-4 electrical symbols A-11
chemical hazard warning A-3 eλPDA detector 4-2
cleaning filters 4-44 emergency-stop button (e-stop) vi, 1-3,
column manager 1-9, 4-2, 4-8 4-7
columns, switching 4-12 Empower
compatible solvents B-1 login 2-4
components methods 2-3
accessing or removing 4-8 user type 1-12
ACQUITY UPLC 1-8 enclosure 1-3, 1-4, 1-6, 4-1
system 1-2, 1-4 compartments 1-6
computer and touch screen 1-11, 2-4 light tower 1-3
configuration file 2-3 locking vi, 1-6
Index-1
sliding drawer 1-7 login
equipment guidelines vi, A-4 advanced access 1-11, 2-1
Empower 2-4
F Tech 2-4, 3-4, 3-5
fan speed 1-6, 1-7 Windows 1-11, 2-4
ferrule installation 4-29
filters, cleaning 4-44 M
firmware, upgrade 4-17 maintaining the system 4-2, 4-3
flow path manufacturing processes 1-2, 1-9, 3-1,
process sample manager 1-10 3-4
front-panel buttons, process sample master AC disconnect v
manager 1-11 materials of construction B-1
full-loop injections 1-11 method editor 3-5
fuses, type 4-45, C-3 method set 2-3
method sets, selecting 2-2
H methods 3-5
handling symbols A-12 mixer 4-43
hexane and tetrahydrofuran B-3 mobile phases, solvents used in
high priority sample 3-4 preparation of B-2
I moving the system 4-12
injection cycles, process sample N
manager 4-4 needle seal
injection valve 4-27, 4-32, 4-35 calibrating 4-16
injections, full-loop 1-11 NuGenesis Scientific Data
inserting a vial 2-4 Management System 1-11
instrument air 1-8
instrument compartment 1-7 O
instrument methods 3-5 online decision-maker file 2-4
intended use vii online prime 4-14
interface, basic software 1-12 online valve control 4-14
ISM classification viii options compartment 1-8
L P
laser, bar-code reader v, C-3 PATROL system
leak sensor 4-18, 4-23 administration 2-1
leak test 4-15 compatible solvents B-1
light tower 1-3, 1-6, 2-2 components 1-2
loading a sample or standards 3-3, 3-4 maintenance and support 1-2
locking the enclosure vi materials of construction B-1
Index-2
software 1-11 replacing
user types 1-11 diluent pump 4-39
PDA detector 4-2 fuses 4-45, 4-46
preventing contamination B-2 mixer 4-43 I
priming 4-35 process pump 4-41
online 4-14 sample loop 4-26
priming valve 4-34 sample pump 4-36
priority sample 3-4 valve cartridge 4-31
process analytical technology (PAT) 1-2
process pump 4-41 S
replacing 4-41 safety advisories A-1
process sample manager (PSM) 1-2 sample carousel 1-11
diluent pump, replacing 4-39 sample door 1-11
disconnecting 4-9 sample loop 4-31
flow path 1-10 replacing 4-26
front-panel buttons 1-11 sample pump 4-36
fuses, replacing 4-46 replacing 4-36
injection cycles 4-4 samples
needle seal, calibrating 4-16 high priority 3-4
process pump, replacing 4-41 process stream 1-2, 1-9, 3-1, 3-4
sample door 1-11 status 2-3
sample loop, replacing 4-26 submitting 1-9, 3-1
sample pump, replacing 4-36 sampling valve 4-33, 4-35
software control 1-11 scientific data management 1-11
submitting samples 1-9 screen saver interface 1-12
valve cartridge, replacing 4-31 SDMS 1-11
process sampling 1-2, 1-9, 3-4 secondary containment 1-7
process valve 4-33 selecting a project 2-2
project, selecting 2-2 selecting method sets 2-2
pump control 4-13 sliding drawer 1-7, 4-8, 4-9
pumps 4-30, 4-36, 4-39, 4-41 software 1-11, 2-2
purpose and audience vii solvent recycling valve 1-7
solvents
R compatible B-1
release lever 4-8 exposure of PATROL system
relocating the system 4-12 components to B-2
remote connection 1-11 managing supply 4-11
removing manifold 1-7
instrument components 4-8 reservoir configurations 1-8
vials 2-4, 3-5
Index-3
supply 1-7 inserting or removing 2-4, 3-3, 3-4,
use in mobile phases B-2 3-5
spare parts 4-4 size 3-2
standards 3-4 viewing the carousel 3-5
status, sample 2-3
submitting samples 3-1 W
switching columns 4-12 walk-up sample submission 3-1
symbols warning symbols A-2, A-4
caution A-4 warnings and cautions, PATROL
electrical A-11 specific 4-6
handling A-12 waste containers 1-7
warning A-2 emptying 4-11
system waste outlet 1-7
administration 2-1 Windows login 1-11, 2-4
components 1-2 Windows Remote Desktop Connection
enclosure 1-3, 4-1 software 1-11, 2-4
relocating 4-12
software 1-11
system enclosure 1-6
T
Tech login 2-4, 3-4, 3-5
tetrahydrofuran and hexane B-3
thermal management 1-6, 1-7
timeouts 2-4, 3-5
touch screen, computer 1-11, 2-4, 3-3
training 1-12
training, for PATROL users vi
TUV detector 4-2, 4-8
types of users, PATROL system 1-11
U
upgrade firmware 4-17
user access 1-12
user type, Empower 1-12
V
valve control 4-13
valves 4-30, 4-32, 4-33, 4-34, 4-35
vials
Index-4

PATROL UPLC Process Analyzer System Guide Rev B

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PATROL UPLC Process

Copyright © Waters Corporation 2010

Waters contact information:

Contacting medium Information

Considerations specific to the PATROL system

Warning: To avoid injury from various causes, allow only

Warning: To avoid contact with hazardous substances, wear clean,

Warning: To avoid personal exposure to biologically hazardous agents,

Warning: To avoid injury, spills, and damage to the equipment, remove

Warning: To avoid electric shock and damage to the equipment, ensure

Warning: To avoid exposure to harmful vapors, connect an exhaust

Warning: To avoid electric shock, use extreme caution when working in

Warning: To avoid exposure to laser radiation, use caution when

Warning: To avoid injury and damage to the enclosure, do not exert a

Caution: To ensure proper drainage, verify that the waste management

Operating this system

Confirms that a manufactured product complies

ABN 49 065 444 751 Australia C-Tick EMC Compliant

Confirms that a manufactured product complies

Audience and purpose

Intended use of the PATROL system

Warning: Do not use the PATROL UPLC Process Analyzer in a manner

ISM Classification: ISM Group 1 Class B

Waters Corporation (Micromass UK Ltd.)

Contacting Waters ............................................................................................... iii

Safety considerations .......................................................................................... iii

Operating this system ......................................................................................... vi

ISM classification .............................................................................................. viii

EC Authorized Representative ......................................................................... ix

1 Analyzing Manufacturing Processes with the PATROL System 1-1

System components .......................................................................................... 1-2

Determining tasks allowed for a user level .............................................. 1-11

2 Configuring the System ........................................................................ 2-1

Logging in to Empower software from the touch screen ........................ 2-4

3 Running Samples ................................................................................... 3-1

Loading standards ............................................................................................ 3-4

Unloading an accepted, analyzed, or rejected vial ................................... 3-5

Configuring methods ....................................................................................... 3-5

4 Maintaining the System ........................................................................ 4-1

Spare parts ......................................................................................................... 4-4

Maintenance considerations .......................................................................... 4-6

Accessing or removing instrument components ....................................... 4-8

xii Table of Contents

Emptying waste containers .......................................................................... 4-11

Relocating the system .................................................................................... 4-12

Maintaining the process sample manager ................................................ 4-13

Cleaning the system enclosure’s exterior ................................................. 4-47

A Safety Advisories .................................................................................. A-1

Caution symbol .................................................................................................. A-4

Electrical and handling symbols ................................................................. A-11

Table of Contents xiii

Items exposed to solvent ................................................................................ B-2

C Specifications ........................................................................................ C-1

Specifications for the process sample manager ....................................... C-3

xiv Table of Contents

1-2 Analyzing Manufacturing Processes with the PATROL System

Solvent and waste

Warning: To avoid injury and damage to the enclosure, do not exert a

System components 1-3

Column Discrete I/O