16.07.

2023

MOLECULAR BIOLOGY & GENETIC ENGINEERING Semester IV

Course Code BBT401 CIE Marks so
Teachln2 Hours/Week [L: T:P: S) 3:0:0:0 SEE Marks so
Total Hours of Peda2ol!V 40 Total Marks 100
Credits 03 Exam Hours 3
Examination tvoe (SEEl Theorv
Cou rs e objectives:
• To acquire the fundamentals of molecuJar biology and genetic engineering principles.
• To u nderstand the p rotocols of isolation of Nucleic acids and their analysis.
• To develop a conceptuaJ a p plication of gene libraries and various interactions.
• To learn the strategies for gene manipuJation, editing technologies and its applications.
Teaching-Leaming Process (General Instructions)
These are sample Strategies, which teachers can use to accelerate the attainment of the various course
outcomes.
• Explanation via real life problem, situation modelling, and deliberation of solutions, hands-on
sessions, reflective and questioning /inquiry-based teaching.
• Instructions with interactions in classroom lectures (physical/hybrid).
• Use of ICT tools, including YouTube videos, related MOOCs, AR/VR/MR tools.
• Flipped classroom sessions (~10% of the classes).
• Industrial visits, Guests talks and competitions for learning beyond the syllabus.
• Students' participation through audio-video based content creation for the syllabus (as
assignments).
• Use of gamification tools (in both physicaJ/hybrid classes) for creative learning outcomes.
• Students' seminars (in solo or group) /oraJ presentations.
ModuJe-1 (8 Hours)
CENTRAL DOGMA OF MOLECULAR BIOLOGY:
Replication of DNA in Prokaryotic cell and Eukaryotic cell Mechanism of action of telomerase, DNA damage,
and repair: Base excision repair, mismatch excision repair, photo-reactivation, nucleotide excision, and Sos
repair. Transcription in the prokaryotic and eukaryotic cell: Initiation, elongation, and termination.
Processing of mRNA. Translation in the prokaryotic and eukaryotic cell: Initiation, elongation, and
termination. Wobble Hypothesis Post-translational modification of proteins. Protein targeting
Module-2 (8 Hours)
GENE REGULATION:
Regulation of gene expression in prokaryotes (lac-operon and trp-operon). Positive and negative gene
regulation, riboswitches. Regulation of gene expression in eukaryotes: Transcriptional control, RNA
processing control, Translational control, and post-translational level control. Hormonal control of gene
expression in eukaryotes (steroid hormone, auxin, and gibberellic acid). Gene silencing: antisense technique,
RNA interference, Ribozymes.
ModuJe-3 (8 Hours)
INTRODUCTI ON TO GENETIC ENGJNEERING:
Basics of Genetic Engineering. Vectors for gene cloning: Cloning and Expression vectors. Plasmids, Phages,
Cosmids, Fosmids, Phagemids, and Artificial chromosomes. ViraJ vectors. MolecuJar tools for gene cloning:
Restriction and Modification systems: Restriction Endonucleases, Methylases, Ligases. Polynucleotide
ldnases, Phosphatases, DNA and RNA polymerases, Reverse transcriptase, Terminal transferase, DNAses
(Extremophiles), Nuclease. RNases, Topoisomerase. Cloning Techniques: Restriction digestion based cloning.
Linkers and adapters, Strategies for cloning TA cloning. Ligase free cloning
ModuJe-4 (8 Hours)
GENE TRANSFER TECHNIQUES:
Physical, chemical and biological methods, Competent cells: Chemical and Electro-competent
Transformation/ transfection in plants and animals. Construction of genomic and cD NA libraries: Screening
of DNA libraries for clone identification. Characterization of clones. Methods of nucleic acid detection;
 16.07.2023

Polymerase chain reaction (PCR) • techniques and requirements, types of PCR, applications. Blotting
techniques (Southern, Northern and Western), Radioactive and non-radioactive labelling of nucleic acids
Module-5 (8 Hoursl
APPLICATIONS OF GENETIC ENGINEERING:
Engineering microbes for the production of antibiotics, enzymes, insulin and monoclonal antibodies.
Transgenic technology for plant and animal improvement, Over expression and Knock out/ knock down
studies, RNAi. Bio pharming- Animals and plants as bioreactors for recombinant proteins. Genome-Editing
Technologies: Types, Principles and Applications; CRISPR- associated protein - Cas 9.
Course outcome {Course Skill Set)
At the end of the course, the student will be able to:
1. Understand the basic concepts of genetic engineering for augmentation of traits.
2. Apply and comprehend the principles of gene manipulation, expression and interaction of genes and
proteins.
3. Evaluate the screening and interaction studies using classical/conventional and high through put
methods.
4. Design the strategies for gene cloning and gene editing.
Assessment Details (both CIE and SEE)
The weightage of Continuous Internal Evaluation (CIE) is 50% and for Semester End Exam (SEE) is
50%. The minimum passing mark for the CIE is 40% of the maximum marks (20 marks out of 50)
and for the SEE minimum passing mark is 35% of the maximum marks (18 out of 50 marks). A
student shall be deemed to have satisfied the academic requirements and earned the credits
allotted to each subject/ course if the student secures a minimum of 40% (40 mar ks out of 100) in
the sum total of the CIE (Continuous Internal Evaluation) and SEE (Semester End Examination)
taken together.

Continuous internal Evaluation:

• For the Assignment component of the CIE, there are 25 marks and for the Internal Assessment
Test component, there are 25 marks.
• The first test will be administered after 40-50% of the syllabus has been covered, and the
second test will be administered after 85-90% of the syllabus has been covered
• Any two assignment methods mentioned in the 22082.4, if an assignment is project-based then
only one assignment for the course shall be planned. The teacher should not conduct two
assignments at the end of the semester if t\.vo assignments are planned.
• For the course, CIE marks will be based on a scaled-down sum of two tests and other methods
of assessment
l.nternal Assessm ent Tes t question pa per is designed to attain the different levels of Bloom's
taxonomy as per the out come defined for the course.

Semester-End Examination:
Theory SEE will be conducted by University as per the scheduled timetable, with common question papers
for the course (duration 03 hours).
1. The question paper will have ten questions. Each question is set for 20 marks.
2. There will be 2 questions from each module. Each of the two questions under a module (with a
maximum of3 sub-questions), should have a mix ortopics under that module.
3. The students have to answer S full questions, selecting one full question from each module.
4. Marks scored shall be proportionally reduced to SO marks
 16.07.2023

Suggested Learning Resources:

Books
• Gene Cloning and DNA Analysis - An Introduction, T.A. Brown, Wiley-Blackwell Science, 7th edition,
2018.
• From Genes to Genomes, Concepts and applications of DNA Technology. Jeremy W. Dale a nd MV
Schantz. 2nd edition, 2018.
• Lewin's genes XII Burlington, Massachusetts: Krebs, Jocelyn E., Goldstei n, Elliott S., Kilpatrick,
Stephen T., Jones & Bartlett Leaming. 2018.
• Molecular Biotechnology - Principles and applications of recombinant DNA, B.R Glick, J.J. Pasternak
and C.L Patten; ASM Press; 6th edn; 2017.
Web links and Video Lectures Ce-Resources):
VTU EDUSAT / SWAYAM / NPTEL / MOOCS / Coursera / MIT-open learning resource
• bttps://www.coursera.org/courses?query=molecular%20biology
• bttps://www.edx.org/learn/molecular-biology
• https://www.classcentral.com/tag/molecular-biology
• https://www.cdc.gov/lahtrain ing/traini ng-coursesjbasic-molecular-biology/ index.html
• https://plLharvard.edu/subject/molecular-biology
• https://onlinecourses.swayam2.ac.in/cec19_bt02/preview
• https://nptel.ac.in/courses/102103013
• https://blo.llbretexts.org/Bookshelves/Microblology/Book%3A_Microblology_(Boundless)/7%3A_
Mlcroblal_Genetics/7.23%3A_Genetic_Engineering_Products/7.23B%3A_Appllcatlons_of_Genetic_E
ngineerlng

Activity Based Learning (Suggested Activities in Class)/ Practical Based learning

• Group Discussion of Case studies
• Model Making and poster presentations
 16.07.2023

BI OSTATISTICS AND TOOLS + LAB Semester IV

Course Code BBT402 CIE Marks so
Teachin2 Hours/Week (L:T:P: S) 3:0:2:0 SEE Marks so
Total Hours of Peda20~ 40 hours Theory+ 8-10 Lab slots Total Marks 100
Credits 04 Exam Hours 03
Examination nature (SEE) Theorv/oractical
Course objectives:
• Understand different types of data and variables.
• Usage of descriptive tools to summarize and display data from public health or medical studies.
• To appropriately choose, define probability distributions such as the Binomial, Poisson and
normal distribution to solve engineering problems.
• To calculate and articulate the associate between two variables.
• Understand the principles of various study designs used in epidemiological studies and explain
their advantages and limitations.
• Practical application of design of experiments in agricultural and laboratory experiments.
• Understand the importance and basic principles of estimation in clinical and translational
research.
• To learn how to formulate hypothesis, perform hypothesis testing and to work with sample data
to make inferences about a population.
T eaching-Learning Process (Gener al Instructions)
These are sample Strategies, which teacher can use to accelerate the attainment of the var ious course
outcomes.
• Explanation via real life problem. situation modelling. and deliberation of solutions, hands-on sessions,
reflectiveand questioning /i nquiry-based teaching.
• Instructions with interactions in classroom lectures (physical/hybrid).
• Use oflCT tools, including YouTube videos, related MOOCs, AR/VR/MR tools.
• Flipped classroom sessions (-10% of the classes).
• Industrial visits, Guests talks and competitions for learning beyond the syllabus.
• Students' participation through audio-video based content creation for the syllabus (as assignments) .
• Use of gamification tools (in both physical/hybrid classes) for creative learning outcomes.
• Students' seminars (in solo or group) /oral presentations.
MODULE-1 (8 hours1
REPRESENTATION OF DATA AND DESCRIPTIVE STATISTICS:
Introduction to Biostatistics, classification of variables, types of data, data collection and sampling
methods, data representation- diagrammatic methods (line diagram, bar diagram, pie chart),
graphical methods (Histogram, frequency polygon, frequency curve, ogive). Measure of central
tendency- mean, median, mode, quartiles, harmonic mean and geometric mean. Measure of
dispersion- mean deviation, quartile deviation, standard deviation and coefficient of variation.
MODULE-2 (8 hours)
BIVARIATE ANALYSIS AND PROBABILITY DISTRIBUTION
Correlation- types, reasons and methods of estimating correlation Spearman's Rank correlation
coefficient and Karl Pearson's coefficient of correlation. Linear Regression analysis, Curve fitting.
Probability distribution-Binominal distribution, Poisson distribution and Normal distribution.
MODULE-3 (8 hours)
EPIDEMIOLOGICAL STUDY DESIGNS:
Observational studies and experi mental studies-case reports and case series, ecological study, cross-
sectional, case-control. cohort study and nested design. historically controlled studies, cross over
studies, randomized controlled design, Selection of Cases and Controls, Types of Controls, Matching in
a Case-Control Study. Measures of Association-Relative Risk & Odds Ratio, Risk difference,
attributable risk. excessive risk incidence, prevalence and incidence rate, prospective and
retrospective studies, Selectivity, specificity and sensitivity, Bias, and Confounding, multiple sources
of variation, Ethical considerations. Replication and repetition, randomisation and blocking, single-
and double-blind experiments.
 16.07.2023

MODULE-4 (8 hours)
DESIGN AND ANALYSIS OF EXPERIMENTS
Randomized complete block design (RCBD) and CRD analysis, Variants of RCBD such as Latin Square,
central composite design, etc., Full factorial experiments, Blocking and Confounding in 2k, Fractional
factorial experiments, Plackett-Burman Designs, Response surface methodology (RSM)
MODULE-5 (8 hours)
INFERENTIAL STATISTlCS FOR CLINlCAL RESEARCHERS
Point estimation, interval estimation- mean and proportion, sample size estimation, sampling
distributions of mean and its properties, testing of hypothesis, type 1 error and type II error, power of
study, test statistics (two tailed only)- Z-test, t-test (Paired and unpaired), chi-squared test Wilcoxon
Signed Rank Test, Wilcoxon-Mann-Whitney Test, ANOVA- One-way and Two way,
PRACTICAL COMPONENT OF CPCC
Sl.N Experiments
0
1 Measurement and Sampling: To select a simple random sample from the population and enter
these data into SPSS/Minitab/or any other statistical software.
2 Diagrammatic & Graphical representation: To plot line diagrams, bar diagram. Pie chart,
Histogram and frequency distribution of the collected data.
3 Summary Statistics: To calculate and interpret summary statistics for the data in your sample.
4 Correlation: Calculation & interpretation of correlation and regression between variables
s Randomization: Use of open-source randomization tools and sample size estimation.
6 Hypothesis testing: To test a hypothesis by determining a significance difference for mean and
proportion.
7 t - test: To use t- test for determining a significance difference between two groups.
8 Chi - Square test; Use of Chi - Square test of independent of Attributes for 2 X 2 contingency
table.
9 Experimental Design: Design and analysis of experiments based on factorial design and
calculate main effect, interaction effect
10 Experimental Design: Design and analysis of mixture experiments using different factors.
11 Experimental Design: Design and analysis of screening experiments using Plackett- Burman
designs
12 Experimental Design: Design and analysis of experiments based on response sur face
methodology (RSM).
Course outcomes (Course Skill Set) :
At the end of the course, the student will be able to:
1. Gather data, present appropriately and perform uni-variate, bi-variate analysis of data.
2. Analyze the statistically designed biological experiments,
3. Draw inferences about the characteristics of population from the samples using parametric and non-
parametric tests.
Assessment Details (both CU: and SEE)
The weightage of Continuous Internal Evaluation (CIE) is 50% and for Semester End Exam (SEE) is
50%. The minimum passing mark for the CIE is 40% of the maximum marks (20 marks out of 50) and
for the SEE minimum passing mark is 35% of the maximum marks (18 out of 50 marks). A student
shall be deemed to have satisfied the academic requirements and earned the credits allotted to each
subject/ course if the student secures a minimum of 40% ( 40 marks out of 100) in the sum total of the
CIE (Continuous Internal Evaluation) and SEE (Semester End Examination) taken together.

CIE for the theory component of the IPCC (maximum marks 50)
 16.07.2023

• IPCC means practical portion integrated with the theory of the course.
• CIE marks for the theory component are 25 ma rks and that for the practical component is 25
marks.
• 25 marks for the theory component are split into 15 marks for two Internal Assessment Tests
(Two Tests, each of 15 Marks with 01-hour duration, are to be conducted) and 10 marks for other
assessment methods mentioned in 22084.2. The first test at the end of 40-50% coverage of the
syllabus and the second test after covering 85-90% of the sylJabus.
• Scaled-down marks of the sum of nvo tests and other assessment methods will be CI E marks for
the theory component of IPCC (that is for 25 marks).
• The student has to secure 40% of25 marks to qualify in the CIE of the theory component of IPCC.
CIE for the practical component of the IPCC

• 15 marks for the conduction of the experiment and preparation of laboratory record, and 10
marks for the test to be conducted after the completion of all the laboratory sessions.
• On completion of every experiment/program in the laboratory, the students shall be evaluated
including viva-voce and marks shall be awarded on the same day.
• The CIE marks awarded in the case of the Practical component shall be based on the continuous
evaluation of the laboratory report Each experiment report can be evaluated for 10 marks. Marks
of all experiments' write-ups are added and scaled down to 15 marks.
• The laboratory test (duration 02/ 03 hours) after completion of all the experiments shall be
conducted for SO marks and scaled down to 10 marks.
• Scaled-down marks of write-up evaluations and tests added will be CIE marks for the laboratory
component of IPCC for 25 marks.
• The student has to secure 40% of 25 marks to qualify in the CIE of the practical component of the
IPCC.
SEE forlPCC
Theory SEE will be conducted by University as per the scheduled timetable, with common question
papers for the course (duration 03 hours)
1. The question paper will have ten questions. Each question is set for 20 marks.
2. There will be 2 questions from each module. Each of the two questions under a module (with
a maximum of3 sub-questions), s hould have a mix of to pics under that module.
3. The students have to answer S full questions, selecting one full question from each module.
4. Marks scored by the student shaJI be proportionally scaled down to SO Marks.
The theory portion of the IPCC shall be for both CIE and SEE, whereas the practical portion will
have a CIE component only. Questions mentioned in the SEE pape r may include questions from
the practical component.
Suggested Learning Resources:
Books
• Fundamentals Of Statistics (Paperback, SC GUPTA) Edition, 6; Publisher, Himalaya, 1984.
• Design of Experiments 1st Edition Bradley Jones, Douglas C. Montgomery/
• Fundamentals ofBiostatistics Paperback - 1 December 2009 by V.B. Rastogi (Author).
Web links a nd Video Lectures (e-Resources):
• VTU EDUSAT / SWAYAM / NPTEL / MOOCS / Cour-sera / MIT-open learning resource
• https://archive.nptel.ac.in/courses/102/106/102106051/
• https://onlinecourses.nptel.ac.in/noc21_mg48/preview
• https://archive.nptel.ac.in/noc/courses/noc18/SEM1/noc18-mg01/
 16.07.2023

Activity Based Learning (Sugges ted Activities in Class)/ Practical Based learning
• Demonstration of sampling
• Two group studies in clinical trials, randomisation and case control experiments.
• https://boltmph.ufl.edu/category/important-concepts/actlvity-learn-by-doing/
 16.07.2023

IMMUNOTECHNOLOGY+ LAB Semester IV

Course Code BBT403 CIE Marks so
Teachini.? Hours/Week {L:T:P: Sl 3:0:2:0 SEE Marks 50
Total Hours of Peda2ol!V 40 hours Theory + 8-10 Lab slots Total Marks 100
Credits 04 Exam Hours 3
Examination nature {SEE1 Theorv/Laboratorv
Course objectives:
• To learn the underlying concepts of molecular and cellular mechanisms involved in the
development and regulation of the immu ne response.
• To describe the ca use for immune System Disorders.
• To learn the techniques of lmmunodiagnostics.
Teaching-Leaming Process (General lnstructions)
Tbese are sample Strategies; tbat teachers can use to accelerate tbe attainment of tbe various course outcomes.
. Explanation via real life problem, situation modelling. and deliberation of solutions, hands-on sessions,
reflective and questioning /inquiry-based teaching.
• Instructions with interactions in classroom lectures (physical/hybrid).
• Use of ICT tools, including YouTube videos, related MOO Cs, AR/VR/MR tools.
• Flipped classroom sessions ( ~10% of the classes) .
. Industrial visits, Guests talks and competitions for learning beyond the syllabus.
. Students' participation through audio-video based content creation for the syllabus (as assignments) .
. Use of gamification tools (in both physical/hybrid classes) for creative learning outcomes.
. Students' seminars (in solo or group) /oral presentations.
MODULE-1 (8 HOURS)
IMMUNE SYSTEM:
Introduction; Immunity-innate and acquired immunity; Haematopoiesis; Cells of immune system - lymphoid cells,
mononuclear cells, granulocytes, dendritic cells & mast cells; organs of immune system - primary and secondary
lymphoid organs; Humeral and Cell mediated immunity; Antigens: Chemical and biological Factors affecting
antigenicity/lmmunogenicity and molecular nature, Haptens, adjuvants; Antibodies: structure and function,
lmmunoglobulin classes and subclasses (isotypic, allotypes, idiotypes and anti-idiotytopic antibodies).

MODULE-2 (8 HOURS)
HUMORAL AND CELL MEDIATED IMMUNITY:
B-lymphocytes and their activation, development and maturation. antibody genes and generation of diversity, Class
Switching mechanism; production of monoclonal antibodies, polyclonal antibodies and applications; Thymus
derived lymphocytes (T cells): activation, development and maturation. thei r ontogeny and types. Major
histocompatibility Complex (MHC) Complex, MHC Class I and II molecules. Antigen processing and presentation
process.

MODULE-3 (8 HOURS)
IMMUNE SYSTEM IN HEALTH AND DISEASE:
Complement system, pathways of complement activation and its functions, Hypersensitivity: GeU and Coombs
classification of Hypersensitivity, Autoimmune disorders-types, animal model and treatment; Immune response to
infections: immunity to viruses, bacteria, fungi and parasites; Immunodeficiency disorders: Primary and secondary
immunodeficiencies (AIDS); Injury and inflammation, Vaccines and their types, production of recombinant vaccine,
Vaccine for hepatitis B surface antigen.

MODULE-4 (8 HOURS)
TRANSPLANTATION AND TUMOR IMMUNOLOGY:
Transplantation and Its classification, Immunologic basis of graft rejection and its mechani.sm, transplantation
antigens, tissue typing, role of MHC molecules in allograft rejection, Clinical transplantations, bone marrow, 1-ISC
transplantation and immunosuppressive therapy; Tumours of the immune system-tumour antigens and immune
response to tumours, tumour immune-therapy.
 16.07.2023

MODULE-5 (8 BOORS)
MOLECULAR IMMU NOLOGY & IMMUNODIAGNOSIS:
Antigen antibody interaction - Precipitation reactions, Agglutination reactions; ABO Blood typing principles;
Principles and applications of ELISA, Radio lmmuno Assay (RJA), western blot analysis, immunoelectrophoresis,
lmmunofluorescence, Fluorescence Activated Cell Sorting (FACS) analysis. Role of stem cells technology in
immunology, Production of humanized monoclonal antibodies (Single chain fragment variable).
PRACTICAL COMPONENT OF IPCC
SLNO Experime nts
1 Agg.lutination Technique: ABO typing
2 Isolation of lymphocytes from peripheral blood
3 Differential counting of WBC
4 Bacterial Agglutination reaction-Wida! test (Tube)
5 Bacterial Agglutination reaction-Wida! test (slide agglutination)
6 Ouchterlony Double Diffusion (ODO)
7 Rocket Immune-electrophoresis (RIEP)
8 Counter-current Immune-electrophoresis (CClEP)
9 Enzyme-linked lmmunosorbent assay (ELISA)
10 Western blotting
11 Complement fLXation test
12 Radial lmmunodiffusion (RJD)
Course outcomes (Course Skill Set) :
At the end of the course, the student will be able to:
1. Outline the molecular and cellular mechanisms involved in the development and regulation of the immune
response,
2. Detail the cause, challenges and treatment for Immune System Pathologies and Dysfunctions.
3. Apply the major immunological laboratory techniques and their application to both clinical analysis and
experimental research.
Assessment Details (both ClE and SEE)
The weightage of Continuous Internal Evaluation (Cl E) is 50% and for Semester End Exam (SEE) is 50%.
The minimum passing mark for the CIE is 40% of the maximum marks (20 marks out of 50) and for the
SEE minimum passing mark is 35% of the maximum marks (18 out of SO marks). A student shall be
deemed to have satisfied the academic requirements and earned the credits allotted to each subject/
course if the student secures a minimum of 40% (40 marks out of 100) in th e sum total of the CIE
(Continuous Internal Evaluation) and SEE (Semester End Examination) taken together.

ClE for the theory compone nt of the lPCC (maximum marks 50)
• LPCC means practical portion integrated with the theory of the cour se.
• ClE marks for the theory component are 25 marks and that for the practical compo nent is 2 5 marks.
• 25 marks for the theory component are split into 1 5 marks for two lnternal Assessment Tests (Two
Tests, each of 15 Marks with 01-hour duration, a re to be conducted) and 10 marks fo r other
assessment methods m e n tio ned in 22084.2. Tbe first test at the end of 40-50% coverage of the
syllabus and the second test after covering 85-90% of the syllabus.
• Scaled-down marks oftbe sum of two tests and other assessment methods will be CIE marks for the
theory component of IPCC (that is for 25 marks).
• The student has to secure 40% of 25 marks to q ualify in the CIE of the theory component of IPCC.
ClE for the practical compo n e nt of the IPCC
 16.07.2023

• 15 marks for the conduction of the experiment and preparation of laboratory record, and 10 marks
for the test to be conducted after the completion of all the laboratory sessions.
• On completion of every experiment/program in the laboratory, the students shall be evaluated
including viva-voce and marks shall be awarded on the same day.
• The CIE marks awarded in the case of the Practical component shall be based on the continuous
evaluation of the laboratory report Each experiment report can be evaluated for 10 marks. Marks of
all experiments' write-ups are added and scaled down to 15 marks.
• The laboratory test (duration 02/ 03 hours) after completion of all the experiments shalJ be
conducted for SO marks and scaled down to 10 marks.
• Scaled-down marks of write-up evaluations and tests added will be CIE marks for the laboratory
component of IPCC for 25 marks.
• The student has to secure 40% of 25 marks to qualify in the CIE of the practical component of the IPCC.
SEE for LPCC
Theory SEE will be conducted by University as per the scheduled timetable, with common question papers
for the course (duration 03 hours)
1. The question paper will have ten questions. Each question is set for 20 marks.
2. There will be 2 questions from each module. Each of the two questions under a module (with a
maximum of 3 sub-questions), s hould have a mix of topics under that module.
3. The students have to answer S full questions, selecting one full question from each module.
4. Marks scored by the student shalJ be proportionally scaled down to SO Marks.
The theory portion of the LPCC shall be for both CIE and SEE, whe r eas the practical portion will
have a CIE component only. Questions mentioned in the SEE pape r may include questions from the
practical component.
Suggested Learning Resources:
Books
• Kuby Immunology by by Jenni Punt, Sharon Stranford, Patricia Jones, Judith A Owen, WH Freeman; 8th ed.
2018.
• Immunology- an Introduction by Tizard Thomson. Saunders College Publising. 1984
• Immunology &lmmunotechnology, Ashim K Chakravarthy, Oxford University Press. 2006.
• lmmundiagnostlcs by SC Rastogi, New Age International. 1996.
• Essential Immunology by Roltt I. Blackwell Scientific Publications, 13th Edition, 2017.
• Immunology: A Short Course Richard Coico, Geoffrey Sunshine Wiley-Blackwell 7th Edition, 2015.
• Understand ing Immunology by Peter Wood, Pearson Education, ZOOL
Web Links and Video Lectures (e-Resources):
VTU EDUSAT / SWAYAM / NPTEL / MOOCS / Coursera / MIT-open learning resource
• https://www.coursera.org/courses?query=immunology
• https://www.edx.org/learn/immunology
• https://www.tangolearn.com/best-immunology-courses-classe.s -online/
• https://www.classcentralcom/course/swayam-immunology-14 ll 7
• https://onlinecourses.nptel.ac.in/noc20_bt43/preview
• https://pll.harvard.edu/course/hmx-immunology?delta=l
Activity Based Learning (Suggested Activi ties in Class)/ Practical Based learning
• AV presentation by students (on specific topics).
• Online surprise quizzes.
• Discussion of case studies based on research findings.
• Model maklne and Poster oresentations.
 16.07.2023

MOLECULAR BIOLOGY & GENETIC ENGINEERING LAB Semester IV

Course Code BBTL404 CIE Marks so
Teachin2 Hours/Week [L:T:P: S) 0:0:2:0 SEE Marks so
Total hours 15 Total marks100
Credits 01 Exam Hours 3
Examination tvoe (SEE) Practical
Course objectives:
• To understand the methods related to isolation, quantification, characterization and amplification of
nucleic acids.
• To perform experiments related to Genetic transformation and recombinants.
• To learn protocols related to separation of Proteins and DNA
SI.NO Exneriments
1 Preparations of common molecular biology lab buffers (TAE, THE, TE, Tris-Hcl etc.)

2 Isolation of genomic DNA plant sources

3 Isolation of genomic DNA microbial or animal sources

4 Agarose gel electrophoresis and quantification of nucleic acids

5 Isolation of total RNA from bacteria/plant/animal samples

6 Estimation of RNA Using Orcinol Method

7 Characterization of DNA by Spectrophotometric Assay and Melting Temperature (Tm)

8 Isolation of plasmid DNA from bacteria

9 Restriction Digestion of plasmid pUC18

10 Amplification of DNA by PCR

11 Separation of Proteins - SOS-PAGE

12 Genetic transformation of E.coli and blue-white screening

13 DIV experiment (To be designed and executed by students themselves)

Course outcomes (Course Skill Set):
At the end of the course the student will be able to:
1. Apply the principles of molecular biology and genetic engineering.
2. Conduct experiments related to isolation, separation, quantification, digestion and amplification of nucleic
acids.
3. Interpret and discuss the outcome of the experiments formally through written reports.

Assessment Details (both CIE and SEE)

The weightage of Continuous lntemal Evaluation (CCE) is 50% and for Semester End Exam (SEE) is 50%.
The minimum passing mark for the CIE is 40% of the maximum marks (20 marks out of 50) and for the
SEE minimum passing mark is 35% of the maximum marks (18 out of 50 marks). A student shall be
deemed to have satisfied the academic requirements and earned the credits allotted to each subject/
course if the student secures a minimum of 40% (40 marks out of 100) in the sum total of the CIE
(Continuous Internal Evaluation) and SEE (Semester End Examination) taken together

Continuous Internal Evaluation (CIE):

CIE marks for the practical course are 50 Marks.
The split-up of CIE marks for record/ journal and test are in the ratio 60:40.
 16.07.2023

• Each experiment is to be evaluated for conduction with an observation sheet and record write-up.
Rubrics for the evaluation of the journal/write-up for hardware/software experiments are
designed by the faculty who is handling the laboratory session and are made known to students at
the beginning of the practical session.
• Record should contain all the specified experiments in the syllabus and each experiment write-up
will be evaluated for 10 marks.
• Total marks scored by the students are scaled down to 30 marks (60% of maximum marks).
• Weightage to be given for neatness and submission of recordj,-vrite-up on time.
• Department shall conduct a test of 100 marks after the completion of all the experiments listed in
the syllabus.
• In a test, test write-up, conduction of experiment, acceptable result, and procedural knowledge will
carry a weightage of 60% and the rest 40% for viva-voce.
• The suitable rubrics can be designed to evaluate each student's performance and learning ability.
• The marks scored shall be scaled down to 20 marks (40% of the maximum marks).
The Sum of scaled-down marks scored in the report write-up/journal and marks ofa test is the total ClE
marks scored by the student
Semester End Evaluation (SEE):
• SEE marks for the practical course are SO Marks.
• SEE shall be conducted jointly by the two examiners of the same institute, examiners are
appointed by the Head of the Institute.
• The examination schedule and names of examiners are informed to the university before the
conduction of the examination. These practical examinations are to be conducted between the
schedule mentioned in the academic calendar of the University.
• All laboratory experiments are to be included for practical examination.
• (Rubrics) Breakup of marks and the instructions printed on the cover page of the answer script
to be strictly adhered to by the examiners. OR based on the course requirement evaluation
rubrics shall be decided jointly by examiners.
• Students can pick one question (experiment) from the questions lot prepared by the examiners
jointly.
• Evaluation of test write-up/ conduction procedure and result/viva will be conducted jointly by
examiners.
General rubrics suggested for SEE are mentioned here, writeup-20%, Conduction procedure and
result in -60%, Viva-voce 20% of maximum marks. SEE for practical shall be evaluated for 100 marks
and scored marks shall be scaled down to SO marks (however, based on course type, rubrics shall be
decided by the examiners)
Change of experiment is allowed only once and 1S% of Marks allotted to the procedure part are to be
made zero.
The minimum duration of SEE is 02 hours
Suggested Lear ning Resources:
• Gene Cloning and DNA Analysis - An Introduction; TA Brown; Wiley-Blackwell Science; 7th edn;201B.
• Laboratory manual for genetic engineering, Vennison S John. Phi learning publishers. 2009.
• Basic techniques in molecular biology by Surzycki, Stefan. Springer Science & Business Media, 2012.
• Basic Techniques in Biochemistry, Microbiology and Molecular Biology: Principles and Techniques by
Aakanchha Jain et al., Springer Protocols Handbooks, 2020.
 16.07.2023

Web links and Video Lectures (e-Res ources):

VTU EDUSAT / SWAYAM / NPTEL / MOOCS / Coursera / MIT-open learning resource
• https://onlinecourses.nptel.ac.in/noc21_bt35/preview
• https://www.mitconbiopharma.com/training/bio-tech-training/certificate-course-in-genetic-engineering/
• https://alison.com/course/understanding-molecular-biology
• h s: s tores.biotecnika.or roducts molecular-biolo -techni ues-certificatlon-course
 16.07.2023

BIOPROCESS PRINCIPLES & STOICHIOMETRY Semester IV

Course Code BBT40 SC CIE Marks so
Teachlne. Hours/Week [L: T:P: S) 3:0:0:0 SEE Marks so
Total Hours of Pedae.ol!V 40 Total Marks 100
Credits 03 Exam Hours 3
Examination tvoe (SEEl Theorv
Cour se objectives:
• Learn fundamentals of chemical calculations and material and energy balance.
• Discuss the material balance aspects involving chemical reactions and without chemical reactions .
• Highlight the energy balance and materiaJ balance for the development ofbioprocess technology.

Teaching-Learning Process (General Instructions)

These are sample Strategies, which teachers can use to accelerate the attainment of the various course
outcomes.
• Explanation via real life problem, situation modelling. and deliberation of solutions, bands-on
sessions, re0ective and questioning /inquiry-based teaching.
• Instructions with interactions in classroom lectures (physical/hybrid).
• Use of ICT tools, including YouTube videos, related MOOCs, AR/VR/MR tools.
• Flipped classroom sessions ( ~ 10% of the classes).
• Industrial visits, Guests talks and competitions for learning beyond the syllabus.
• Students' participation through audio-video based content creation for the syllabus (as
assignments).
• Use of gamification tools (in both physical/hybrid classes) for creative learning outcomes.
• Students' seminars (in solo or group) /oral presentations.
Modul e-1 £8 Hours)
BASIC CHEMICAL CALCULATIONS AND MATERIAL BALANCE:
Concept of atom and mole, expressing composition of mixtures in Solids, liquids and gases. Expressing
composition of mixtures and solutions - Percentage by weight percentage, mole percentage and Volume
percentage; Normality, Morality, Molality. Generalized material balance equations for distillation, absorption,
extraction, crystallization, mixing. drying & evaporation.
Module-2 (8 Hours)
MATERIAL BALANCE WITHOUT CHEMICAL REACTIONS AND FUELS
Material balances calculation in Distillation, Absorption, Extraction, Crystallization, Drying. Mixing and
Evaporation Operations, Fuels - types of fuels. (solid, liquid and gaseous fuel), relevance to biofuels,
characteristics of fuels, Ultimate and proximate analyses of fuels.
Module-3 £8 Hours)
MATERIAL BALANCE INVOLVING CHEMICAL REACTIONS:
Material balances calculation involving bypass, recycle and operations. Generalized material balance
equations, Principles of stoichiometry, Definitions of limiting and excess reactants, fractions and percentage
conversion, yield and percentage yield, Selectivity, unit process - neutralization, oxidation, nitration,
hydrolysis, and problems relating to these unit processes.
Module-4 (8 Hours)
ENERGY BALANCE:
General energy balance equation for steady state. Heat capacity, estimation of beat capacity for solids,
liquids, gases and their mixtures. Enthalpy, Standard Heat of formation, standard beat of reaction, Standard
heat of combustion and calorific value, Calculation of heat of reaction at elevated temperature
Module-5 (8 Hours)
BIO PROCESS PRINCIPLES & STOICHIOMETRY OF BIOPROCESS:
Historical development ofbloprocess technology; Bioprocess principles and operations, generalized process
now sheets. General material balance equation for steady state (for manufacture of penicillin and ethanol) -
outline of a bioprocess and the various (upstream and downstream) unit operations involved in
bioprocesses. Stoichiometry of microbial growth and product formation.
 16.07.2023

Course outcome (Course Skill Set)

At the end of the course, the student will be able to:
1. Discuss the significance of material and energy balance for bioprocess technology.
2. Solve problems related to material and energy balance to give solutions for bioprocess development
3. Develop the flowsheet for general processes operating in bioprocess industry.
4. Appreciate the stoichiometry of microbial growth and product formation involved in bioprocess
technology.
Assessment Details (both CIE and SEE)
The weightage of Continuous Internal Evaluation (CIE) is 50% and for Semester End Exam (SEE) is
50%. The minimum passing mark for the CIE is 40% of the maximum marks (20 marks out of 50)
and for the SEE minimum passing mark is 35% of the maximum marks (18 out of 50 marks). A
student shall be deemed to have satisfied the academic requirements and earned the credits
allotted to each subject/ course if the student secures a minimum of 40% (40 marks out of 100) in
the sum total of the CCE (Continuous Internal Evaluation) and SEE (Semester End Examination)
taken together.

Continuous Internal Evaluation:

• For the Assignment component of the CIE, there are 25 marks and for the Internal Assessment
Test component, there are 25 marks.
• The first test will be administered after 40-50% of the syllabus has been covered, and the
second test will be administered after 85-90% of the syllabus has been covered
• Any two assignment methods mentioned in the 22082.4, if an assignment is project-based then
only one assignment for the course shall be planned. The teacher should not conduct two
assignments at the end of the semester if two assignments are planned.
• For the course, CIE marks will be based on a scaled-down sum of two tests and other methods
of assessment
Internal Assessment Test question paper is designed to attain the different levels of Bloom's
taxonomy as per the outcome defined for the course.

Semester-End Examination:
Theory SEE will be conducted by University as per the scheduled timetable, with common question papers
for the course (duration 03 hours).
1. The question paper will have ten questions. Each question Is set for 20 marks.
2. There will be 2 questions from each module. Each of the two questions under a module (with a
maximum of3 sub-questions), sh ould have a mix of topics under that module.
3. The students have to answer 5 full questions, selecting one full question from each module.
4. Marks scored shall be proportionally reduced to SO marks
Suggested Leaming Resources:
Books
• Basic Principles and Calculations in Chemical Engineering by David Himmelblau, PHI
• Bioprocess Engineering by Shuler and Kargi, Prentice Hall.
• Stoichiometry by Bhatt and Vora, Tata McGraw Hill, 2004.
• Chemical Process Calculations by R. Asokan, University Press, 2011
• Principles of Biochemistry by David L Nelson (Editors), W. H. freeman and company.
• Bioprocess Engineering Principles by Pauline Doran, Academic Press.
• Biochemical Engineering Fundamentals by J E Bailey & D. F. Ollis, McGraw Hill.
• Calculations by I. H. Segel, John Wiley & Sons
Web links a nd Video Lectures (e-Res ources):
 16.07.2023

• https://nptel.ac.in/courses/102105064
• https://nptel.ac.in/courses/102106053
• https://onlinecourses.nptel.ac.in/noc21_btl7 /preview
• https://www.youtube.com/watch?v=-ZrvfgfiOZs
• https://archive.nptel.ac.in/courses/ 103/103/103103153/
Activity Based Learning (Suggeste d Activities in Class)/ Practical Based learning
• AV presentation by students (on topics as per choice of the teacher).
• Online tools for surprise quizzes.
• Collection of case studies based on research findings
• Poster presentations on specific case studies.
 16.07.2023

BIOPESTICIDES AND BIOFERTILlZERS Semester rv

Course Code BBT456D CIE Marks so
Teachin£ Hours/Week [L: T:P: S) 1:0:0:0 SEE Marks so
Total Hours of Peda£Ol!V 15 Total Marks 100
Credits 01 Exam Hours 1
Examination tvoe (SEE) Theorv
Cou rse objectives:
• To familiarize the students on biopesticides and biofertilizers that are free from harmful chemicals
and are more environment friendly for the purposes of achieving better crop production

Teaching-Leaming Process (General Instructions)

These are sample Strategies, which teachers can use to accelerate the attainment of the various course
outcomes.
• Explanation via real life problem, situation modelling. and deliberation ofsolutions, hands-on
sessions, reflective and questioning /inquiry based teaching.
• Instructions with interactions in classroom lectures (physical/hybrid).
• Use of ICT tools, including YouTube videos, related MOOCs, AR/VR/MR tools.
• Flipped classroom sessions ( ~ 10% of the classes).
• Industrial visits, Guests talks and competitions for learning beyond the syllabus.
• Students' participation through audio-video based content creation for the syllabus (as assignments).
• Use of gamification tools (in both physical/hybrid classes) for creative learning outcomes.
• Students' semina rs (in solo or group) /oral presentations.
Module-1 [3 Hours}
PATHOGENS AND PESTS MANAGEMENT:
Pathogens and Pests Management, Natural Enemies, Reduviids and Their Merits in Biological Control, Weaver
Ants and Biocontrol of the Nuisance Pest Luprops tristis (Coleoptera: Tenebrionidae), Ground Beetles
(Coleoptera: Carabidae): Their Potential as Bio-agents in Agroecosystems, Eco-friendly Control of Three
Common Mosq uito Larvae Species by Odonata Nymphs, Spiders as Potential Eco-friendly Predators Against
Pests.
Module-2 (3 Hours)
BIOFERTILlZERS:
Types and importance of biofertilizers, Biopesticides and bioagents In agriculture and organic farming system,
History of biofertllizers production Classification of biofertilizers microorga nisms used In biofertilizers
production
ModuJe-3 (3 Hoursl
NITROGEN FIXATION:
Concept of Nitrogen fixation. Structure and characteristic features of bacterial biofertilizers - Azotobacter,
Bacillus, Rhizobium; Cynobacteriol biofertilizers - Anabaena, and fungal biofertilizers - VAM.
Module-4 (3 Hours)
BIOPESTJCIDES:
General account of microbes used as bioinsecticides and their advantages over synthetic pesticides, Bacillus
thuringiensis, Mechanism of phosphate solubillzation and phosphate mobilization, K solubilization. Botanicals:
botanical pesticides, and biorationales. Botanicals and their uses. Plant Essential Oils and Pest Management
Module-5 (3 Hours)
PRODUCTION AND QUALITY CONTROL :
Strain selection, sterlllzation, growth and fermentation, mass production of biofertllzers. Storage, shelf life,
quality control and marketing. Factors influencing the efficacy of biofertillzers/Biopesticides, FCO
specifications and quality control of biofertllizers. Application technology for seeds, seedlings, tubers, etc.
 16.07.2023

Course outcome (Course Skill Set)

At the end of the course the student will be able to:
1. Correlate the principles of Microbiology towards Blofertilizers and Bioinsectlcldes.
2. Comprehend Pest-Plant Interactions and apply the same In Agriculture.
3. Understand strain selection and apply the same to scale up production of Biofertilizers and
Bioinsecticides.
Assessment Details (both CIE and SEE)
The weightage of Continuous Internal Evaluation (CIE) is 50% and for Semester End Exam (SEE) is
50%. The minimum passing mark for the ClE is 40% of the maximum marks (20 marks out of SO)
and for the SEE minimum passing mark is 35% of the maximum marks (18 out of SO marks). A
student shall be deemed to have satisfied the academic req uirements and earned the credits allotted
to each subject/ course if the student secures a minimum of 40% (40 marks out of 100) in the sum
total of the CIE (Continuous Internal Evaluation) and SEE (Semester End Examination) taken
together.

Continuous inte rnal Examination (CJ£)

• For the Assignment component of the CIE, there are 25 marks and for the Internal Assessment
Test component, there are 25 marks.
• The first test will be administered after 40-50% of the syllabus has been covered, and the second
test will be administered after 85-90% of the syllabus has been covered
• Any two assignment methods mentioned in the 22082.4, if an assignment is project-based then
only one assignment for the course shall be planned. The teacher should not conduct two
assignments at the end of the semester if two assignments are planned.
• For the course, Cl E marks will be based on a scaJed-down sum of two tests and other methods of
assessment
Internal Assessment Test question pa per is d esigned to attain the different levels of Bloom's taxonomy
as per the outcome defined for the course.

Semeste r End Examinations (SEE)

SEE paper shall be set for 50 questions, each of the 01 marks. The pattern of the question paper is MCQ
(multiple choice questions). The time aUotted for SEE is 01 hour. The student has to secure a minimum of
35% of the maximum marks meant for SEE.
OR
MCQ (Multiple Choice Questions) are preferred for 01 credit courses, however, if course content demands the
general question paper pattern that followed for 03 credit course, then
1. The question paper wiU have ten questions. Each question is set for 10 marks.
2. There will be 2 questions from each module. Each of the two questions under a module may or may
not have the sub-questions (with maximum sub-questions of 02, "vith marks distributions 5+5, 4+6,
3+7).
3. The students have to answer 5 full questions, selecting one full question from each module.

Suggested Leaming Resources:

Books
• Biofertllizer Technology, Marketing and Usage, Motsara, 1.M.R., Bhattacharyya, P. and Srivastava, B.
1995.
• Biofertilizers in Agriculture and Forestry, Subba Rao, N.S. Oxford and IBH. Publ. Co., New Delhi. 1993.
• Formulation of Microbial Biopesticides: Beneficial microorganisms, nematodes and seed treatments,
H. D. Burges, Spingerlink, 1998.
• Biofertilizer and Biopesticide by Shalini Suri (Author)Aph Publishing Corporation 2011.
• Biological control of insect pest suppression. Coppel H.C. and J.W. Martin. Springer. 1977.
• Biofertllizers and Biopesticides by Krishnendu Acharya, Surjit Sen, Techno World; 2019.
 16.07.2023

Web links and Vid eo Lectures (e-Resources):

VTU EDUSAT / SWAYAM / NPTEL / MOOCS / Coursera / MIT-open learning resource Features of Risk
• http://courseware.cutm.ac.ln/courses/certificate-course-bio-fertilizer-preparation/
• https://onlinecourses.swayam2.ac.in/cec21_ag03/preview
• https://www.udemy.com/course/basics-of-fertilizers/
• https://www.youtube.com/watch?v=Qxv-lEGucFs
• https://knowledge.unccd.int/e-learning-course-organic-fertilizer-sustainable-agriculture
• http://www.digimatin/nptel/courses/video/102105058/LSS.html
Activity Based Learning (Suggested Activities in Dass)/ Practical Based l earning.
• Study of biological agents like anabena, nostoc, VAM and Rhizobium
• Group Discussion of Case studies
• Model Making and poster presentations
 16.07.2023

BIOLOGY FOR ENGINEERS Semester IV

Course Code BBOK407 CIE Marks so
Teachin2 Hours/ Week [L: T:P: S) 3:0:0:0 SEE Marks so
Total Hours of Peda2ol!V 40 Total Marks 100
Credits 03 Exam Hours 3
Examination tvoe (SEEl Theorv
Cou rse objectives:
• To familiarize the students with the basic biological concepts and their engineering
applications.
• To enable the students with an understanding ofbiodesign principles to create novel
devices and structures.
• To provide the students an appreciation of how biological systems can be re-designed as
s u bstitute products for natural systems.
• To motivate the studen ts to develop interdisciplinary vision of biological engineering.

T eaching-Learning Process (General Instructions)

These are sample Strategies, which teachers can use to accelerate the attainment of the various course
outcomes.
• Explanation via real life problem, situation modelling. and deliberation of solutions, hands-on
sessions, reflective and questio ning /inquiry-based teaching.
• Instructions with interactions in classroom lectures (physical/hybrid).
• Use of ICT tools, including YouTube videos, related MOOCs, AR/VR/MR tools .
• Flipped classroom sessions ( ~10% of the classes).
• Indus trial visits, Guests talks and competitions for learning beyond the syllabus.
• Students' participation through audio-video based content creation for tbe syllabus (as
assignments).
• Use of gamification tools (in botb physical/hybrid classes) for creative learning outcomes.
. Students' seminars (in solo or group) /oral presentations.
Module-1 rs Hours}
LNTRODUCTION TO BIOLOGY:
The cell: the basic unit or life. Structure and functions or a cell. The Plant Cell and animal cell, Prokaryotic
and Eukaryotlc cell. Stem cells and thei r application. Biomolecules: Properties and functions of
Carbohydrates. Nucleic acids. proteins, lipids. Importance or special biomolecules: Enzymes (Classification
(with one example each), Properties and functions), vitamins and hormones.

Modul e- 2 (8 Hours)
BIOMOLECULES AND THEIR APPLICATIONS (QUALITATIVE):
Carbohydrates (cellulose-based water filters. PHA and PLA as bioplastics). Nucleic acids (DNA Vaccine for
Rabies and RNA vaccines for Covid19, Forensics - DNA fingerprinting), Proteins (Proteins as food - whey
protein and meat analogs, Plant based proteins), lipids (biodiesel, cleaning agents/detergents), Enzymes
(glucose-oxidase in biosensors, lignolytic enzyme in bio-bleacbing).
Module-3 rs Hours}
HUMAN ORGAN SYSTEMS AND BIO DESIGNS (QUALI TATIVE):
Brain as a CPU system (architecture, CNS and Peripheral Nervous System, signal transmission, EEG,
Robotic arms for prosthetics. Engineering solutions for Parkinson's disease). Eye as a Camera system
(architecture of rod and cone cells, optical corrections, cataract, lens materials, bionic eye). Heart as a
pump system (architecture, electrical signalling • ECG monitoring and heart related issues, reasons for
blockages of blood vessels, design of stents, pace makers, defibrillators). Lu ngs as purification system
(architecture, gas exchange mechanisms, spirometry, abnormal lung physiology - COPD. Ventilators,
Heart-lung machine). Kidney as a filtration system (architecture, mechanism of filtration, CKD, dialysis
systems).
 16.07.2023

Module-4 (8 Hours)
NATURE-BIOINSPIREO MATERIAl.S AND MECHANISMS (QUALITATIVE):
Echolocation (ultrasonography, sonars), Photosynthesis (photovoltaic cells, bionic teal). Bird flying (GPS and
aircrafts), Lotus leaf effect (Super hydrophobic and self-cleaning surfaces), Plant burrs (Velcro), Shark skin
(Friction reducing swim suits), Kingfisher beak (Bullet train). Human Blood substitutes • hemoglobin-based
oxygen carriers (HB0Cs) and perflourocarbons (PFCs).
Module-5 (8 Hours}
TRENDS IN BIOENGINEERING (QUALITATIVE):
Muscular and Skeletal Systems as scaffolds (architecture, mechanisms, bioengineering solutions for
muscular dystrophy and osteoporosis), scaffolds and tissue engineering. Bioprinting techniques and
materials, 3D printing of ear, bone and skin. 30 printed foods. Electrical tongue and electrical nose in food
science, DNA origami and Biocomputing, Bioimaging and Artificial Intelligence for disease diagnosis. Self-
healing Bioconcrete (based on bacillus spores, calcium lactate nutrients and biomineralization processes)
and Bioremediation and Biomining via microbial surface adsorption {removal of heavy metals like Lead,
Cadmium, Mercury, Arsenic).
Cou rse ou tcome {Course Skill Set)
At the end of the course, the student will be able to :
1. Elucidate the basic biological concepts via relevant industrial applications and case studies.
2. Evaluate the principles of design and development, for exploring novel bioengineering projects.
3. Corroborate the concepts ofbiomimetics for specific requirements.
4. Think critically towards exploring innovative biobased solutions for socially relevant problems.
Assessment Details (both ClE and SEE)
The weightage of Continuous Internal Evaluation (ClE) is 50% and for Semester End Exam (SEE) is
50%. The minimum passing mark for the C1E is 40% of the maximum marks (20 marks out of SO)
and for the SEE minimum passing mark is 35% of the maximum marks (18 out of SO marks). A
student shall be deemed to have satisfied the academic requir ements and earned the credits
allotted to each subject/ course if the student secures a minimum of 40% (40 marks out of 100) in
the sum total of the ClE (Continuous Internal Evaluation) and SEE (Semester End Examination)
taken together.

Continuous Internal Evaluation:

• For the Assignment component of the CIE, there are 25 marks and for the Internal Assessment
Test component, there are 25 marks.
• The first test will be administered after 40-50% of the syllabus has been covered, and the
second test will be administered after 85-90% of the syllabus has been covered
• Any two assignment methods mentioned in the 22082.4, if an assignment is project-based then
only one assignment for the course shall be planned. The teacher should not conduct two
assignments at the end of the semester if two assignments are planned.
• For the course, CIE marks will be based on a scaled-down sum of two tests and other methods
of assessment
lntemal Assessment Tes t question paper is designed to attain the differe nt levels of Bloom's
taxonomy as per the outcome defined for the course.

Semester-End Examination:
Theory SEE will be conducted by University as per the scheduled timetable, with common question papers
for the course (duration 03 hours).
1. The question paper will have ten questions. Each question Is set for 20 marks.
2. There will be 2 questions from each module. Each of the two questions under a module (with a
maximum of3 sub-questions), s hould have a mix of topics under that module.
3. The students have to answer 5 full questions, selecting one full question from each module.
4. Marks scored shall be proportionally reduced to 50 marks
 16.07.2023

Suggested Learning Resources:

Books
• Biology for Engineers, Rajendra Singh C and Rathnakar Rao N, Rajendra Singh C and Rathnakar Rao
N Publishing, Bengaluru, 2023.
• Human Physiology, Stuart Fox, Krista Rompolskl, McGraw-Hill eBook. 16th Edition, 2022
• Biology for Engineers, Thyagarajan S., Selvamurugan N., Rajesh M.P., Nazeer R.A., Thilagaraj W.,
Barathi S., and Jaganthan M.K., Tata McGraw-HUI, New Delhi, 2012.
• Biology for Engineers, Arthur T. Johnson, CRC Press, Taylor and Francis, 2011
• Biomedical Instrumentation, Leslie Cromwell, Prentice Hall 2011.
• Biology for Engineers, Sohini Singh and Tanu Allen, Vayu Education oflndia, New Delhi, 2014.
• Biomimetics: Nature-Based Innovation, Yoseph Bar-Cohen. 1st edition, 2012, CRC Press.
• Bio-Inspired Artificial Intelligence: Theories, Methods and Technologies, D. Floreano and C.
Mattiussi, MIT Press, 2008.
• Bioremediation of heavy metals: bacterial participation, by C R Sunilkumar, N Geetha A C
Udayashankar Lambert Academic Publishing, 2019.
• 30 Bioprinting: Fundamentals, Principles and Applications by Ibrahim Ozbolat. Academic Press,
2016.
• Electronic Noses and Tongues in Food Science, Maria Rodriguez Mende, Academic Press, 2016
Web links a nd Video Lectures Ce-Resources):
• https://nptel.ac.in/courses/121106008
• https://freevideolectures.com/course/4877/nptel-biology-engineers-other-non-biologists
• https://ocw.mit.edu/courses/20-020-introduction-to-biological-engineering-design-spring-2009
• https://ocw.mit.edu/courses/20-0lOj-introductlon-to-bioengineeri.ng-be-OlOj-spring-2006
• https://www.coursera.org/courses?query=biology
• https://onllnecourses.nptel.ac.in/nocl9_ge31/preview
• https://ww,.v.classcentral.com/subject/biology
• https://www.futurelearn.com/courses/biology-basic-concepts

Activity Based Learning (Suggested Activities in Qass)/ Practical Based learning

• Group Discussion of Case studies.
• Model Making and seminar/poster presentations.
• Design of novel device/equipment like Cellulose-based water filters, Filtration system.