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    Official - ncm0176 - Yeast and Mold Agar - Technical Specifications - en Us

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    Luki Wahyuning Tiyas
    The document provides a technical specification sheet for Yeast and Mold Agar, which is recommended for isolation and maintenance of yeasts and molds. It describes the intended use, typical formulation, preparation instructions, quality control specifications, test procedures and limitations of the agar.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Official - ncm0176 - Yeast and Mold Agar - Technical Specifications - en Us

    Uploaded by

    Luki Wahyuning Tiyas
    21 views2 pages
    The document provides a technical specification sheet for Yeast and Mold Agar, which is recommended for isolation and maintenance of yeasts and molds. It describes the intended use, typical formulation, preparation instructions, quality control specifications, test procedures and limitations of the agar.

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    Mold and yeast

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    official_ncm0176_yeast-and-mold-agar_technical-specifications_en-us

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    The document provides a technical specification sheet for Yeast and Mold Agar, which is recommended for isolation and maintenance of yeasts and molds. It describes the intended use, typical formulation, preparation instructions, quality control specifications, test procedures and limitations of the agar.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    21 views2 pages

    Official - ncm0176 - Yeast and Mold Agar - Technical Specifications - en Us

    Uploaded by

    Luki Wahyuning Tiyas
    The document provides a technical specification sheet for Yeast and Mold Agar, which is recommended for isolation and maintenance of yeasts and molds. It describes the intended use, typical formulation, preparation instructions, quality control specifications, test procedures and limitations of the agar.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Technical Specification Sheet

    Yeast and Mold Agar (NCM0176)


    Intended Use
    Yeast and Mold Agar is recommended for the isolation and maintenance of yeasts and molds. This
    medium can also be used for the detection of wild yeasts in beer. Yeast and Mold Agar is not intended
    for use in the diagnosis of disease or other conditions in humans.

    Description
    Yeast and Mold Agar is prepared according to the formulation published by Wickerham and was
    formulated for the selective isolation of yeasts from mixed cultures containing bacteria and molds. The
    selectivity of this medium may be enhanced through acidification or through addition of selective agents.
    Yeast and Mold Agar should be sterilized without pH adjustment and sterile acid added to the medium
    cooled to 45 - 50°C. Acidified Yeast and Mold Agar should not be heated. Antibiotics may be aseptically
    added to sterile media. Other fungistatic materials may also be added to Yeast and Mold Agar to
    eliminate molds and permit enumeration of yeasts to mixed populations.
    Revision: 1 Effective Date: 10/27/2022

    Typical Formulation
    Enzymatic Digest of Gelatin 5.0 g/L
    Yeast Extract 3.0 g/L
    Malt Extract 3.0 g/L
    Dextrose 10.0 g/L
    Agar 20.0 g/L
    Final pH: 6.2 ± 0.2 at 25°C
    Formula may be adjusted and/or supplemented as required to meet performance specifications.

    Precaution
    Refer to SDS

    Preparation
    1. Suspend 41 grams of the medium in one liter of purified water.
    2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
    3. Autoclave at 121°C for 15 minutes.
    4. Cool to 45-50°C.

    Quality Control Specifications


    Dehydrated Appearance: homogeneous, free-flowing, beige

    Prepared Appearance: Clear and yellow

    Expected Cultural Response: Cultural response on YM Agar incubated aerobically at 30°C and
    examined for growth after 2 - 5 days.
    Technical Specification Sheet

    Approx. Expected Results


    Microorganism
    Inoculum (CFU) Recovery Response
    Aspergillus brasiliensis ATCC® 16404 Point Inoculation Growth White mycelium black spores
    Candida albicans ATCC® 10231 50-200 >70% Cream colonies
    Escherichia coli ATCC® 25922 4 Quad Streak Growth ----
    Lactobacillus fermentum ATCC® 9338 4 Quad Streak Growth ----
    Penicillium roquefortii ATCC® 10110 Point Inoculation Growth Grey - green mycelium
    Pichia fermentans ATCC® 10651 4 Quad Streak Growth Off-white to beige
    Saccharomyces cerevisiae ATCC® 9763 4 Quad Streak Growth Off-white to beige
    Saccharomyces pastorianus NCYC 185 4 Quad Streak Growth Off-white to beige
    The organisms listed are the minimum that should be used for quality control testing.

    Test Procedure
    1. Inoculate YM Agar with the appropriate sample for the presence of yeasts, molds, or aciduric
    microorganisms.
    Revision: 1 Effective Date: 10/27/2022

    2. Incubate at 30 ± 2°C for 2 to 7 days.


    Results
    Examine plates for growth. Record YM Agar results as colony forming units (CFU) per volume of sample.

    Expiration
    Refer to expiration date stamped on container. The dehydrated medium should be discarded if not free
    flowing, or if appearance has changed from the original color. Expiry applies to medium in its intact
    container when stored as directed.

    Limitations of the Procedure


    1. Due to nutritional variation, some strains may be encountered that grow poorly or fail to grow on this
    medium.
    2. Acidified YM Agar should not be reheated.

    Storage
    Store dehydrated culture media at 2-30°C away from direct sunlight. Once opened and recapped, place
    container in a low humidity environment at the same storage temperature. Protect from moisture and light
    by keeping container tightly closed.

    References
    1. 1951. U. S. Dept. Agricult. Tech. Bull. No. 1029.
    2. 1939. J. Tropical Med. Hyg. 42:176.
    3. Jong, S. C., and M. J. Edwards. 1991. American Type Culture Collection Catalog of filamentous fungi.
    18th ed. American Type Collection, Rockville, MD.

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