Dry Heat and Gaseous Sterilization Methods

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DRY HEAT AND GASEOUS STERILIZATION

QUESTIONS

1.Mention the principle of autoclave

2.list the phases of autoclave.

3.advantages and disadvantages of autoclave.

4.Dry heat sterilization - sterilization control.

5.Sterilization control of dry heat sterilization.

6.Advantages and disadvantages of ETO sterilization

7.what is flash sterilization?

8.mention the sterilization control of plasma sterilization.

9.Disinfecion of operation theatre.


1.Principle: Water boils when its vapor pressure equals that of the surrounding
atmosphereWhen the atmospheric pressure is raised, the boiling temperature is also raised.At
normal pressure, water boils at 100°C but when the pressure inside a closed vessel increases,
the temperature at which water boils also increases

2.Conditioning phase:air in the chamber is completely displaced by the steam produced The
steam pressure rises and when it reaches the desired level , the safety valve opens
Exposure phase: The holding periodis count,which is about 15 minutes in most cases Exhaust
phase:the steam sterilizer is cooled till it reaches atmospheric pressure.

3.advantages:1)It is low cost and fast 2) It is nontoxic and leaves no by-product Disadvantage 1)
Heat can damage and corrode some metals 2) Higher temperature shorten the life of
instruments.

4.Sterilization control: Spores of Bacillus atrophaeusis used as biological indicator as they are
more resistant to dry heat than are Geobacillus stearothermophilus spores.

5.Preconditioning: air is removed from the chamber and vacuum is created. Then the physical
conditions (temperature, pressure and humity) for sterilization are set
Sterilization: sterilization is achieved in 4-5 hours at 38°C or 1 hour at 55°C

Aeration: The ETO residues left on surgical instruments may be toxic to the patients and staff,
extensive aeration for 8-12 hours is necessary to remove residual ETO

6.Advantages of ETO: (i) Large chamber capacity(ii) suitable for heat sensitive items, (iii) high
penetration power(iv) non- corrosive to plastic, metal and rubber materials

Disadvantages: (i) ETO is highly inflammable and carcinogenic, (ii) supplied in a 10-20%
concentration; mixed with inert gas, (iii) long duration (iv) high cost of instrument

7.Flash sterilization is a modification of conventional steam sterilization, designed to be used at


emergency. (134°C for 3-10 minutes)in an unwrapped condition

8. Sterilization control of plasma sterilization: Spores of Geobacillus stearo thermophilus is used


as a biological indicator to check the effectiveness of sterilization.

9.Disinfection of Operation Theatre:Environmental cleaning in OT minimizes only remove


patients' and HCWs' exposure to potentially infectious micro- organisms.

1) Surface disinfection: Cleaning with a cleansing agent, followed by disinfection by aldehyde-


based disinfectant. 2) Fogging: Also called aerial disinfection, involves spraying of a disinfectant
(e.g. glutaraldehyde, H₂O, or QAC based product) with the help of a fogger machine

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