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Microbiology 26Mar2009 DO NOT DISTRIBUTE - 1 Lab 1 Gram Reactions 1) Which of the following is NOT Gram-positive? a) Listeria monocytogenes b) Streptococcus pneumoniae c) Staphylococcus saprophyticus d) Klebsiella pneumonia e) Streptococcus pyogenes 2) a) b) c) d) e) 3) a) b) c) d) e) Which of the following is rod shaped? Staphylococcus aureus Neisseria gonorrhoeae Bacillus anthracis Borrelia burgdorferi Moraxella catarrhalis Which of the following is the best description oflisteria monocytogenes? Gram-negative spirals Gram-negative cocci Gram-negative rods Gram-positive cocci Gram-positive rods

4) Which of the following would be the best magnification power for viewing bac teria such as Escherichia coli with an oil immersion microscope? a) 1x b) 10x c) 100x d) 1,000x e) 10,000x Lab 2 Gram-positive Cocci 1) Which of the following tests would involve looking for bubbling after applicati on of

the a) b) c) d) e)

test agent? Catalase Coagulase Bile esculin Bacitracin Optochin

2) If a bacteria ferments mannitol salt, which is a peachy red color, what colo r will it change to if the test is positive? a) Blue b) Red c) Green d) Yellow e) Black 3) PT: a) b) c) Streptococci pneumoniae is the number one cause of all of the following EXCE Adult meningitis Neonatal meningitis Community acquired pneumonia

Microbiology 26Mar2009 DO NOT DISTRIBUTE - 2 d) Otitis media in children 4) In the hemolysis test on a blood agar plate, an incomplete hemolysis will sho w streaks of what color? a) Blue

b) c) d) e)

Clear Green Yellow Black

5) Which of the following Gram-positive cocci is catalase positive and coagulas e positive? a) Staphylococci epidermidis b) Staphylococci saprophyticus c) Staphylococci aureus d) Streptococci pyogenes e) Streptococci agalactiae 6) Which of the following Gram-positive cocci is! (gamma) hemolytic? a) Streptococci pneumonia b) Viridans strep c) Streptococci pyogenes d) Streptococci agalactiae e)Enterococcus Lab 3 Gram-negative Bacteria 1) Which of the following Gram-negative rods does not ferment glucose and is oxi dase positive? a) E. coli b) P. vulgaris c) P. aeruginosa d)Salmonella e)Shigella 2) In the lactose fermentation MacConkey test, what color would indicate a posi tive test? a) Black b) Pink c) Blue d) Purple e) Green 3) Which of the following Gram-negative rods ferments glucose, is oxidase negat ive, ferments lactose, and is indole positive (tryptophan metabolizing)? a) E. coli b)Shigella c) K. pneumoniae d)Salmonella e) P. vulgaris 4) A child presents with vomiting and bloody stools. A bacterial culture yields

Gramnegative rods that are H2S negative. Which of the following is most likely? a) E. coli

Microbiology 26Mar2009 DO NOT DISTRIBUTE - 3 b)Shigella c) K. pneumoniae d)Salmonella e) P. vulgaris 5) Which of the following can swarm on a plate, making it difficult to distingu ish colonies? a) E. coli b)Shigella c) K. pneumoniae d)Salmonella e) P. vulgaris 6) a) b) c) d) e) 7) a) b) c) d) What is the primary virulence factor for E. coli? Inflammatory response cAMP inducing toxin Toxin inhibits protein synthesis LPS Superantigen What is the primary virulence factor for P. aeruginosa? cAMP inducing toxin Capsule Spore Teichoic acid

e) Type III secretion system Lab 4 Chocolate Agar and CHO Fermenters 1) Bacteria are cultured and found to be Gram-negative diplococci that grow on c hocolate agar but not blood agar. Which of the following is the most likely? a)Staphylococci b)Streptococci c)Mycoplasma d)Haemophilus e)Neisseria 2) Which of the following is a dextrose (glucose) fermenter and is a maltose an d sucrose non-fermenter? a) H. influenza b) H. parainfluenza c) S. pyogenes d) N. gonorrhea e) N. meningitis 3) A sexually active male presents with a painful sore (ulcer on his penis) and inguinal lymphadenopathy. Culture reveals Gram-negative coccobacilli that grow on chocola te agar but not blood agar. Which of the following is the most likely? a) C. trachomatis b) T. pallidum c) N. gonorrhea d) H. ducreyi

Microbiology

26Mar2009 DO NOT DISTRIBUTE - 4 e) H. influenza 4) What is the required growth factor associated with the bacteria in the previ ous question? a) Heme (X) b) NAD (V) c) Heme and NAD (X and V) d) Cysteine e) Cysteine and heme (X) 5) Which of the following describes Moraxella catarrhalis and Neisseria gonorrh oeae in regards to Trio-tube (CHO) fermentation, where yellow is positive and peach is n egative? a) Yellow for dextrose, peach for maltose, peach for sucrose b) Yellow for dextrose, yellow for maltose, peach for sucrose c) Yellow for dextrose, yellow for maltose, yellow for sucrose d) Peach for dextrose, yellow for maltose, yellow for sucrose e) Peach for dextrose, peach for maltose, yellow for sucrose 6) a) b) c) d) e) 7) a) b) c) d) e) What is the primary virulence factor for N. gonorrhea? Capsule Teichoic acid Pili Toxin M protein What is the primary virulence factor for H. influenza? Capsule Teichoic acid Pili Toxin M protein

8) A 1-year-old infants presents with a fever after her parents noticed she was unusually drowsy. The physician notices neck rigidity and occasional seizures. Bacterial c ulture reveals Gram-negative coccobacilli that grow on chocolate agar but not blood aga r. a) H. parainfluenza b) H. influenza c) N. gonorrhea d) S. pyogenes e) N. meningitis

9) What is the required growth factor associated with the bacteria in the previ ous question? a) Heme (X) b) NAD (V) c) Heme and NAD (X and V) d) Cysteine e) Cysteine and heme (X) 1 Introduction to Medical Microbiology 1.1) Which of the following is smallest in physical size? a) Viruses

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Sections show all prev next 19 Mechanisms of Bacterial Pathogenesis 22 Staphylococcus 23 Streptococci 24 Enterococcus 25 Bacillus 26 Listeria 28 Nocardia 29 Mycobacteria 30 Neisseriae

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Almunaleezaa Leezaaleft a comment THAAAAAAAAAAAAAAAANKS 03 / 29 / 2011 . .

This document has made it onto the Rising list! 08 / 12 / 2010 . .

Corry Qyleft a comment The quiz was really helpful in assessing your microbiology knowledge. Thanks a b unch!!!! :D 05 / 07 / 2010 . .

rebenPleft a comment thanks 09 / 22 / 2009 . .

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