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UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS

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International General Certificate of Secondary Education

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CO-ORDINATED SCIENCES 0654/05
Paper 5 Practical Test
May/June 2006

2 hours
Candidates answer on the Question Paper.
Additional Materials: As listed in Instructions to Supervisors

READ THESE INSTRUCTIONS FIRST

Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use a pencil for any diagrams, graphs or rough working.
Do not use staples, paper clips, highlighters, glue or correction fluid.

Answer all questions.

Chemistry practical notes for this paper are printed on page 12.
At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.

For Examiner’s Use

1

Total

This document consists of 11 printed pages and 1 blank page.

IB06 06_0654_05/3RP
 UCLES 2006 [Turn over
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For
Examiner's
1 This question is about two digestive enzymes, amylase and pepsin. Pepsin breaks down Use
protein in the stomach. Amylase breaks down starch in the mouth and small intestine. The
experiment will find out whether pH affects the activity of these enzymes.

Label six test-tubes with the numbers 1 to 6 and place them in a test-tube rack.
Pepsin Test
The enzyme will be added to a cloudy protein suspension. If the protein is digested it
becomes clear.

Using clean syringes for each substance make up the first three test-tubes as follows:
• Put 2 cm3 protein solution into tubes 1, 2 and 3.
• Add 1 cm3 water to tube 1.
• Add 3 cm3 water to tube 3.
• Add 1 cm3 hydrochloric acid to tube 2.
• Add 2 cm3 pepsin solution to tubes 1 and 2.
• Put the tubes into the water bath provided.

Amylase Test
The enzyme will be added to a starch solution. If the starch is broken down, iodine will stay
light brown when tested with the reaction mixture.
Prepare the next three tubes as follows:
• Put 2 cm3 starch solution into tubes 4, 5 and 6.
• Add 1 cm3 water to tube 4.
• Add 3 cm3 water to tube 6.
• Add 1 cm3 hydrochloric acid to tube 5.
• Add 2 cm3 amylase solution to tubes 4 and 5.
• Put these tubes in the water bath.

(a) (i) Leave all the tubes in the water bath for 5 minutes. During this time complete the
contents column for each tube in Fig. 1.1. [1]

tube number contents observations

1 protein, water, pepsin

Fig. 1.1

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For
Examiner's
(ii) After the end of 5 minutes carry out the following. Use

• Remove all 6 test-tubes from the water bath and place them in the test-tube
rack.
• Carefully look at tubes 1, 2 and 3 and record in Fig. 1.1 whether their
appearance is clear or cloudy.
• Place 3 drops of iodine on a white tile making sure they are far apart from each
other (see Fig. 1.2).

Fig. 1.2

• Dip a glass rod into tube 4. When you bring it out of the tube it will have a drop
of the reaction mixture on it. Transfer this drop so that it mixes with one of the
drops of iodine. Observe the colour and record it in Fig. 1.1.
• Wipe the glass rod clean with a paper towel. Test the contents of tubes 5 and 6
in the same way and record the results in Fig. 1.1. [4]

(b) (i) Which of the six tubes showed digestion?

[1]

(ii) Which enzyme worked better in conditions of low pH?

[1]

(iii) Explain your observations for tube 5 in terms of enzyme action.

[1]

(iv) Why were tubes 3 and 6 included in the experiment?

[1]

© UCLES 2006 0654/05/M/J/06 [Turn over

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For
Examiner's
(c) This experiment is to find out if amylase is a protein. Use

• Using the piece of material provided, prepare a lining for the filter funnel, then place
the lined funnel in a clean test-tube in a test-tube rack.
• Using a syringe, transfer 3 cm3 amylase solution into a different test-tube. Heat the
amylase solution until it boils. Filter the boiled amylase by pouring it into the filter
funnel.
• Allow the filtrate to cool for a few minutes.
• Add 1 cm3 acid and 2 cm3 pepsin to the filtrate. Put the test-tube into the water bath
at 40 oC for 5 minutes. After this time remove the tube from the water bath and
place it in the test-tube rack.

(i) Describe the appearance of the amylase after boiling.

What had happened to the amylase?

[2]

(ii) Describe the appearance of the mixture after warming in the water bath at 40 oC.
Explain what has happened.

appearance

explanation

[2]

(iii) Give two pieces of evidence from the experiment that indicate that amylase is a
protein.

[2]

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For
Examiner's
2 You are going to find the density of plasticine by measuring the mass and volume by two Use
different methods. The two sets of results for the mass, together with the result for the
volume will be used to calculate the density each time.

(a) Shape the plasticine into a cube or rectangular block. Use a ruler to find the dimensions
of the block and record them in the space below. Your measurements should be in
centimetres. Use these values to calculate the volume of the plasticine.

1
2
3

Fig. 2.1

measurements

1. cm 2. cm 3. cm

volume = cm3 [2]

(b) Weigh the block of plasticine to the nearest gram.

mass of plasticine = g [1]

(c) Calculate the density of plasticine from the figures obtained in (a) and (b).
Show your working.

density = g / cm3 [1]

(d) You will now determine the volume of the same piece of plasticine by measuring the
displacement of water in a measuring cylinder.

(i) Place enough water in the measuring cylinder to allow the plasticine to be totally
immersed. Read and record the volume of water.

volume before adding plasticine = cm3

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For
Examiner's
(ii) Carefully place the plasticine into the water and record the new volume. You can Use
alter the shape of the plasticine to allow it to fit into the measuring cylinder.

volume after adding plasticine = cm3

(iii) Using these measurements, calculate the volume of the plasticine.

volume of plasticine = cm3 [1]

(e) You are now going to find the mass of the plasticine by using a second method.
You will need to remove it carefully from the measuring cylinder and dry off any excess
water from its surface.

(i) Balance the metre rule on the pivot and record the value of the balance point as
shown in Fig. 2.2.

balance point

metre 0 100
rule
pivot

Fig. 2.2

balance point = cm [1]

(ii) Hang the 25 g mass from the10 cm mark on the rule. Record the distance, d1, of the
10 cm mark from the pivot.

d1 = cm

(iii) Attach a thread to the plasticine and hang this on the other end of the rule. Adjust
the position of the plasticine until the rule balances. Record the distance, d2, from
the pivot. See Fig. 2.3. Make sure the balance point is the same as in (e)(i).

d2 = cm [1]

metre 0 100
rule
d1 d2 plasticine
pivot
25 g mass

Fig. 2.3

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For
Examiner's
(iv) Use the following equation to calculate the mass of the plasticine. Use

d1 × 25 = d2 x mass of plasticine

Show your working.

mass = g [2]

(f) Using the mass from (e)(iv) and the volume from (d)(iii), calculate the density of
plasticine. Show your working.

density = g / cm3 [2]

(g) Which measurement of volume (a) or (d)(iii) is the more accurate?

Explain your answer.

[2]

(h) Briefly describe how you would find the density of plasticine at a temperature of 80 oC.

[2]

© UCLES 2006 0654/05/M/J/06 [Turn over

 8
For
Examiner's
3 You are going to find the volume of gas given off when substance X is heated. Use

(a) Clamp the test-tube containing substance X and place in a stand. Place the bung
containing the delivery tube in the test-tube. Place enough water in the trough to cover
the end of the delivery tube.

Completely fill the measuring cylinder with water. Place your hand over the end, invert
the cylinder and stand it in the trough of water before removing your hand. Clamp the
cylinder in the stand.

Refer to Fig. 3.1 to see how the apparatus should appear.

The end of the delivery tube should be under the measuring cylinder. It may be
necessary to hold this in place while heating is carried out.

delivery
substance X tube measuring cylinder

water trough

Fig. 3.1

Adjust the flame of the burner to about 10 cm high or less. Heat substance X and
collect the bubbles in the cylinder. When there are no more bubbles, remove the
delivery tube from the water and stop heating. You must make sure the delivery tube is
out of the water before you stop heating to avoid water sucking back into the hot tube.

Leave the tube and contents to cool down. You will need the cooled solid for part (d).

(i) Record the mass of solid X used.

mass = g [1]

(ii) Record the volume of gas collected in the measuring cylinder.

volume of gas collected = cm3 [2]

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For
Examiner's
(b) (i) Remove the cylinder from the clamp. Allow any remaining water to come out. Add Use
about 5 cm3 of limewater to the gas in the cylinder. Gently shake the limewater and
record any observation.

observation

name the gas identified by this test [2]

(ii) The first few bubbles collected were not the gas identified. What were they?

[1]

(c) Explain why “sucking back” may take place and why this would be dangerous.

[2]

(d) Dissolve a little of the cooled residue from the tube in which X was heated, in about
5 cm3 of water. Add a few drops of Universal Indicator. Record the colour and estimate
the pH.

colour

pH = [2]

(e) You are to carry out two tests on a fresh sample of substance X.
Dissolve substance X in about 10 cm3 of warm water.

(i) Place about 2 cm3 of the solution into a test-tube and add dilute hydrochloric acid.
Record your observations.

observations

name of gas [2]

(ii) To another portion add a few drops of Universal Indicator. Record the colour and
estimate the pH.

colour

pH = [1]

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For
Examiner's
(f) The teacher calculated the expected volume of gas using the mass of substance X. The Use
volume actually collected was less than this volume. He said that some of the gas must
have dissolved in the water. Suggest another way of collecting and measuring the
volume without using water. You should draw a diagram of any apparatus that would be
used.

[2]

© UCLES 2006 0654/05/M/J/06

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BLANK PAGE

0654/05/M/J/06
 12

CHEMISTRY PRACTICAL NOTES

Test for anions

anion test test result

carbonate (CO32–) add dilute acid effervescence, carbon dioxide
produced
chloride (Cl–) acidify with dilute nitric acid, then white ppt.
[in solution] add aqueous silver nitrate
nitrate (NO3–) add aqueous sodium hydroxide ammonia produced
[in solution] then aluminium foil; warm carefully
sulphate (SO42–) acidify then add aqueous barium white ppt.
[in solution] chloride or aqueous barium nitrate

Test for aqueous cations

cation effect of aqueous sodium hydroxide effect of aqueous ammonia

ammonium (NH4+) ammonia produced on warming -
copper (II) (Cu2+) light blue ppt., insoluble in excess light blue ppt., soluble in excess
giving a dark blue solution
iron(II) (Fe2+) green ppt., insoluble in excess green ppt., insoluble in excess
iron(III) (Fe3+) red-brown ppt., insoluble in excess red-brown ppt., insoluble in excess
zinc (Zn2+) white ppt., soluble in excess giving white ppt., soluble in excess, giving
a colourless solution a colourless solution

Test for gases

gas test and test results

ammonia (NH3) turns damp litmus paper blue
carbon dioxide (CO2) turns limewater milky
chlorine (Cl2) bleaches damp litmus paper
hydrogen (H2) “pops” with a lighted splint
oxygen (O2) relights a glowing splint

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reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.

University of Cambridge International Examinations is part of the University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department
of the University of Cambridge.

© UCLES 2006 0654/05/M/J/06