Brain

Introduction to Neuroscience
INTRODUCTION TO NEUROSCIENCE
Open Edition
VALERIE HEDGES
Michigan State University Libraries

East Lansing
Introduction to Neuroscience by Valerie Hedges is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike
4.0 International License, except where otherwise noted.
CONTENTS
Acknowledgments ix
Image Credits x
Introduction 1
Valerie Hedges
Part I. Introduction to Neuroscience
1. What is Neuroscience? 5
Part II. Cells of the Nervous System Structure & Function
2. Cells of the Nervous System: The Neuron 25

3. Cells of the Nervous System: Glia 43
4. Visualizing Cells of the Nervous System 50
5. Ion Movement 56
6. Membrane Potential 63
7. The Membrane at Rest 77
8. Electrical Activity in Neurons 88
9. Action Potentials 98
10. Voltage Clamp 116
Part III. Neuronal Communication
11. Synapse Structure 131

12. Steps in Synaptic Signaling 139
13. Neurotransmitter Identification and Action: Ionotropic Receptors versus 150
Metabotropic Receptors
14. Introduction to Small Molecule Neurotransmitters 167
15. Neurotransmitters: Acetylcholine 172
16. Neurotransmitters: Amino Acid Neurotransmitters (Glutamate, GABA, 182
Glycine)
17. Neurotransmitters: Biogenic Amines (Serotonin, Histamine) 191
18. Neurotransmitters: Catecholamines (Dopamine, Norepinephrine, 196
Epinephrine)
19. Neurotransmitters: Atypical Neurotransmitters 206
20. Drug and Toxin Effects 213
Part IV. Nervous System Organization
21. Anatomical Terminology 221

22. Brain Structure Differentiation 229
23. Brain Anatomy 243
24. Additional Structures of the Nervous System 264
25. Imaging the Living Brain 277
26. Spinal Cord Structure 290
27. Peripheral Nervous System 304
Part V. Sensory Systems
28. General Principles of Sensory Systems 335

29. Visual System: The Eye 339
30. Visual System: Central Processing 363
31. Auditory System: The Ear 396
32. Auditory System: Central Processing 423
33. Vestibular System 443
34. Gustatory System 455
35. Olfactory System 470
36. Somatosensory Systems 490
37. Touch: The Skin 497
38. Touch: Central Processing 513
39. Pain and Nociception 538
Part VI. Motor Systems
40. Spinal Motor Control and Proprioception 563

41. Spinal Reflexes 591
42. Central Pattern Generators 602
43. Planning of Movement 609
44. Execution of Movement 618
45. Basal Ganglia 631
46. Neurodegenerative Diseases- Motor 660
Part VII. Behavior
47. Neurodegenerative Disease- Non Motor 683

48. Motivated Behavior: Feeding Behavior 695
49. Motivated Behavior: Reward Pathway 723
50. Motivated Behavior: Sexual Behavior 732
51. Drug Addiction 738
52. Sexual Differentiation 762
53. Sex Steroid Hormones 773
54. Organizational and Activational Effects of Steroid Hormones 781
55. Social Bonding 794
56. Sleep and EEG 804
57. Circadian Rhythms 816
58. Emotions: Overview 825
59. Emotions: Fear 835
60. Neural Control of Stress 845
61. HPA Axis 851
62. Psychiatric Disorders: Anxiety 861
63. Psychiatric Disorders: Affective Disorders- Major Depression Disorder 871
64. Psychiatric Disorders: Affective Disorders- Bipolar Disorder 883
65. Psychiatric Disorders: Schizophrenia 887
66. Memory Systems 897
67. Molecular Mechanisms of Memory: Aplysia 920
68. Molecular Mechanisms of Memory: Hippocampus 927
Additional Review Answers 949

Images of Animations 963
ACKNOWLEDGMENTS | IX
ACKNOWLEDGMENTS
The creation of this open educational resource was made possible through the Michigan State
University Libraries and through a grant from the Michigan State University OER Program. Special
thanks goes to Regina Gong for her support throughout this process and to the Michigan State
University Libraries editors and accessibility liaisons.
X | IMAGE CREDITS
IMAGE CREDITS
Cover Image
Cover design provided by Valerie Hedges.
Cover brain artwork by Valerie Hedges.
INTRODUCTION | 1
INTRODUCTION
Valerie Hedges
Introduction to Neuroscience is designed for undergraduate students enrolled in a typical 2-semester

sequence of introductory neuroscience courses. This book specifically targets students enrolled in
Introduction to Neuroscience 1 and Introduction to Neuroscience 2 at Michigan State University
and primarily contains topics covered in those courses.
This book will guide students through concepts, such as: the structure and function of nervous
system cells and nervous system structures; the different sensory systems; motor systems; motivation
and reward; stress; sex and the brain; emotions; nervous system disorders; learning; and memory.
This text has been remixed and revised from two different open educational resources aimed at
undergraduate neuroscience students:
Foundations of Neuroscience by Casey Henley, Michigan State University
The Open Neuroscience Initiative by Austin Lim, DePaul University
In addition, new original content has been added to supplement what was provided in the above
texts. The text includes many images and animations throughout and will be divided into shorter
chapters that focus on a single topic. As this text is meant for undergraduate students, the writing is
aimed at students that have not taken a neuroscience course before. Neuroscience terminology will be
defined throughout the text through the use of the embedded Glossary terms to help with ease of
reading. Each chapter will end with an interactive quiz for student self-evaluation of the content.
Find errors or have suggestions? Please email [email protected]
About the Author
Valerie Hedges
MICHIGAN STATE UNIVERSITY
2 | INTRODUCTION
INTRODUCTION TO NEUROSCIENCE | 3
PART I
INTRODUCTION TO
NEUROSCIENCE
4 | INTRODUCTION TO NEUROSCIENCE
WHAT IS NEUROSCIENCE? | 5
1.
WHAT IS NEUROSCIENCE?
What is Neuroscience?
Neuroscience is the study of the nervous system, the
collection of nerve cells that interpret all sorts of
information which allows the body to coordinate activity
in response to the environment. Resources
The study of neuroscience has taught us that the brain
is a complicated organ with several connection routes,
both between different bodily organs and within itself.
Some of those connections communicate information • Scientist links to learn
down towards the body, such as signals that allow us to more
control the movements of our muscles or to change the • Glossary Terms
activity of our internal organs. Other connections ascend • Key Takeaways
into the brain, conveying all sorts of information from the • Test Yourself
world around us into a representation of our
surroundings. Still, other routes communicate between
brain areas, such as when the sudden detection of a threat
passes through our visual system and turns into a “get ready” signal that then prepares the rest of our
body for conflict. Because of this complex system of communication, the nervous system can be
thought of as a series of highways and roads that connect different cities (organs).
The nervous system conveys all of these different types of information using a combination of
electrical and chemical signals. The main active cellular units of the nervous system, the neurons,
are highly sensitive to changes in their environment. A wide variety of chemicals called
neurotransmitters are responsible for passing information between neurons.
6 | WHAT IS NEUROSCIENCE?
Figure 1.1. Brain

size comparison
between
different
vertebrate
mammals. Brain
images are
shown for a rat,
cat, chimpanzee,
human, and
dolphin. There
are similarities in
the layout of the
structure of the
brain across all
species.
Neuroscience is an integrative field of study

Realistically, our modern understanding of “neuroscience” is a combination of several academic
disciplines, all using their strengths to understand some aspect of the nervous system. Because of this
integrative nature, it is possible to study neuroscience from many different perspectives, each of them
more fitting for answering different types of questions. These “angles” of analysis are described below.
Biology
At the root of the study is biology. Whenever you are studying living processes, such as
learning, visual perception, or consciousness, you dip into the realm of biology. The broad field
of biology can be subdivided into smaller, more precise categories. Molecular neurobiologists
study proteins and gene regulation, cellular neurobiologists examine how networks of neurons
communicate with one another, and cognitive neuroscientists study the underlying causes of
behaviors. Understanding neuroscience involves genetics, such as the autosomal dominant
neurodegenerative condition Huntington’s disease. Other biological sub-disciplines, such as
ecology and evolution, are also considered in neuroscience as well, such as the parasite
Toxoplasma, which changes an animal’s response to fearful stimuli, allowing the organism to
reproduce as it moves through different species in the food web.
Psychology
Psychology provided the earliest explanations about the brain and ideas about the origin of
the mind. Some questions in this field branched off from philosophy as people began thinking
about the “mind–body problem”, the discussion that centered around the question of whether
a function as complex as consciousness could result from the activity of a clump of cells.
Psychologists also wondered whether parts of the brain in isolation have different properties
than when those parts are working together. This property, called emergence, is the idea that
the whole is greater than the sum of its parts. Psychologists examine neuroscience from a top-
down view, aiming questions at understanding the whole organism before looking at smaller
components of the organism (compare this with biological approaches, often a bottom-up view
that starts at the level of cells or molecules).
Chemistry
Chemistry is a strong influencer of nervous system function—just ask anyone who forgot
their morning cup of coffee! We use a variety of endogenous (originating from within the
body) chemicals that act as signaling molecules, allowing communication between cells. These
chemicals exist in many different structures, which determine their function; some are acidic
while others are basic, some are polar, others are fat-soluble, and some are even gases. The
nervous system is also highly sensitive to influence by exogenous chemicals (meaning they
originate from outside the body), such as caffeine and cocaine.
Physics
Many principles of physics can be observed through the functioning of neurons. For example,
neurons maintain a negative electrical charge, usually measured on the scale of tens of millivolts
(a millivolt is a thousandth of a volt.) The main way for neurons to send signals depends on a
temporary change in this voltage; this signal is called an action potential. This change in voltage
is brought on by the movement of charged ions across the cell membrane, and they closely
follow the rules of magnetism: opposite charges attract while like charges repel.
Mathematical Modeling
The field of computational neuroscience has grown from the use of mathematical modeling
to describe or predict some aspect of the nervous system. If our current estimates are correct,
we have around 86 billion neurons in the brain, a number so large that it is difficult to
conceptualize. It would be nearly impossible to understand that many components of a system

without taking advantage of the sheer mathematical strength of a computer.
Healthcare Providers
Healthcare providers, like neurologists and psychiatrists, work from a different angle. They
coordinate closely with researchers to apply scientific knowledge from the field or laboratory to
treat patients, thus using biological principles as therapies. For example, neurologist Dr. Oliver
Sacks used his knowledge of the dopamine neurotransmitter system to treat patients with a
paralysis-like condition in the 1960s, leading to the development of levadopa treatment for
Parkinson’s disease. Other healthcare providers use imaging strategies like a CT scan to assess
the extent of a head injury or the location of a brain tumor, while an EEG can be helpful for the
diagnosis of epilepsy.
Engineers
Engineers help develop the tools needed to understand questions in neuroscience, such as the
patch clamp rig or electron microscope, highly specialized pieces of lab equipment. They also
work closely with healthcare providers to translate science into therapy, such as the deep brain
stimulator devices for the treatment of conditions such as Parkinson’s disease. Collectively,
all the people who participate in neuroscience in some way are united by their interest in the
workings of the body. Because of the overwhelming complexity of the nervous system, there are
many questions still unanswered. The continual appearance of new questions in neuroscience
keeps us wondering, inspires curiosity, and promises a multitude of fascinating career paths for
centuries to come.
Figure 1.2 Areas

of study that
contribute to the
field of
Neuroscience.
Many fields
contribute to our
understanding of
modern
neuroscience
including biology,
psychology,
chemistry,
mathematical
modeling,
healthcare
providers, and
engineers.
How do we learn about neuroscience?
Experimental design
The gold standard in science is the use of experimental design. In an experiment, the scientist uses
a stepwise process of developing a research question and hypothesis, then answering that question
by performing tests. The main goal of an experiment is to establish a causal relationship between
one factor that is being changed, the independent variable, and the factor that is influenced, the
dependent variable. A well-designed experiment has variables that are carefully controlled, which
minimizes the influence of extraneous variables, often called confounding variables. The influence of
confounding variables can be eliminated by comparing the experimental group with a control group,
a group that is as similar as possible in every way except for the manipulation of the independent
variable. Importantly, subjects or patients are generally assigned to the experimental or control group
at random.
Figure 1.3 The

Scientific
Method. The
Scientific Method
starts with the
development of a
research
question or
observation that
will lead to a
research topic
area and
hypothesis. The
hypothesis is
tested through
experimentation
and analysis. The
results of the
experiment are
then
communicated
via a report,
which can
further support
additional
observations or
questions.
Case Studies
Another strategy is the case study, a highly detailed description of a single patient and their condition.
A case study documents the details regarding a specific deficit or enhancement and is an opportunity
to examine individuals with very rare conditions, which are useful for informing about the functions
of different brain structures. Like a quasi-experimental study, case studies only show correlation, not
causation. It is difficult to generalize the findings from a case study to the population at large.
Perhaps the most famous case study in all of neuroscience is the 1848 story of the railroad worker
Phineas Gage. Gage was a construction foreman working on the railroad when an unfortunate
explosive workplace accident caused a iron rod to be driven through his left frontal lobe, largely
destroying it. Remarkably, Gage survived this accident and lived another 12 years. However, Gage’s
acquaintances described subsequent changes in his personality, teaching us that one of the functions
of this area of the brain is regulating our inhibitions.
Figure 1.4 Image

of Phineas Gage
and his site of
injury. The figure
on the left is an
image of Phineas
Gage holding the
iron rod that
caused his injury.
The image on the
right shows a
Magnetic
Resonance Image
rendering of the
location of the
rod in the injury.
The rod entered
below the left
eye and
damaged much
of the left frontal
cortex.
Case studies can be helpful for the development of hypotheses that can later be tested experimentally.
For example, consider another famous case study of Patient HM, the man who had his left and right
hippocampus surgically removed and couldn’t create certain types of memory. A research question
based on this case study might be: “Is the hippocampus needed for the creation of navigational
memory?” Then, an experimental study could be performed in rodents, where we surgically remove
the hippocampus (experimental group) or a different part of the brain (control group) and see how
well the rodents perform on a memory task.
The Use of Animals in Research

Though there are many ways that we can directly study humans through experimentation or case
studies, it is often impossible to test every question in humans. Instead of always studying humans,
scientists often use nonhuman model organisms, the most common organisms being the worm C.
elegans, fruit flies (Drosophila melanogaster), zebrafish (Danio rerio), song birds, mice, rats, and
macaque monkeys.
Figure 1.5
Experimental
Animal Models.
Images of C.
elegans (worms),
Drosophila
melanogaster
(fruit flies), mice,
rats, and
Macaque
monkeys.
The closer we move towards the human, the more similarities the model organism shares with us. Of
the commonly used model organisms, macaque monkeys are the non-humans that are most similar to
humans. We share 93% of our genetic material with macaques, but we still have different metabolic
and physiological processes, and our behaviors are much different from theirs. Ethical constraints
prevent us from performing experiments that may cause physical or psychological harm if performed in
humans. We would never conduct a test on humans to assess what concentration of neurotoxin leads
to brain damage (these experiments aren’t done very frequently in nonhumans anyway). Invertebrates,
such as worms and fruit flies are not as heavily regulated by ethics oversight committees, allowing
scientists to conduct a wider set of experiments on these animals.
Our moral responsibilities toward animal subjects are that:
1. Animals should only be used in worthwhile experiments.

2. All steps are taken to minimize pain and distress.
3. All possible alternatives to animal research are considered.
Research facilities at colleges and universities are monitored by an Institutional Care and Use
Committee (IACUC). The IACUC consists of fulltime veterinarians, scientists, and community
members. They must follow federal laws when approving animal research.
Experimental Preparations
Performing an experiment in an intact, living organism, whether human or nonhuman, is

described as an in vivo (Latin meaning “within life”) preparation. The main strength of this
strategy is that the data collected here are more predictive of the human condition, which is
one of the main goals of biomedical research. However, the in vivo preparation has
challenges, because thousands of variables within a living system are uncontrolled or still
unknown. There are also very strict ethical limitations on the nature of experiments that
can be done in vivo.
On the other hand, an in vitro (Latin meaning “within glass”) preparation is an experiment
performed on cultured cells or isolated molecules of DNA, RNA, or protein. These
preparations have the opposite strengths and weaknesses of in vivo preparations. They
allow for extremely good control over variables, but the results are less reliable in
translating to a therapy. The regulations on these experiments are much more lax compared
to in vivo experiments; most of the regulatory guidelines are to protect the experimenter
rather than the patient or the experimental subject.
Falling in between these two preparations is an ex vivo experiment. In this kind of

experiment, a section of the living organism is taken, such as a slice of brain, a tissue biopsy,
or a detached frog leg. The strengths and limitations of these experiments are somewhere
in between that of the other two preparations.
Figure 1.6 Experimental Preparations. Details of the in vivo, ex vivo, and in vitro preparations can be
found in the text. The in vivo preparation has the least control over variables, but increased ability to
predict therapeutic potential and increased strictness of ethical regulations. The ex vivo preparation
has more control over variables, and decreasing ability to predict therapeutic potential and strictness
of ethical regulations. The in vitro preparation has the most control over variables but the least ability
to predict therapeutic potential and the least strict ethical regulations.
What neuroscience is not

As complex as the brain is, naturally misconceptions make their way into popular culture. It’s valuable
to address these myths about neuroscience and explain the evidence that refutes these statements .
Myth 1: “We only use 10% of our brain.”

This wildly inaccurate statistic has been the foundation for several fictional movies, TV shows, and
books. The truth is that we use every part of the brain, and most of our brain is active most of the
time—just not at the same time. Neurologist V.S. Ramachandran uses a great analogy to describe the
fallacy of this myth: does a traffic light only use 33% of its lights? A properly functioning traffic light
will use all three lights at very precise times. The activity of the brain is closely regulated by multiple
mechanisms which prevent unusual electrical activity. In fact, if too many cells were active at the
wrong times, just like a traffic light showing both green and red, chaos ensues—one cause of seizures is
excessive neural activity.
Myth 2: “Forming memories causes new neurons to be

born.”
Another misconception is the idea that each new cell in our brain represents a new memory. While
we are far from understanding the process of exactly how memories are formed in the brain, we
do have a few clues. Most likely, memories are stored at the sites of close contact between neurons,
called synapses. Changes in ways neurons connect and communicate with one another is likely the
mechanism behind how memories are formed and stored, rather than the creation of new neurons.
Even though the process of cell reproduction is halted in the majority of adult neurons, we are still
capable of new neuronal growth, a process called neurogenesis. A few brain areas in particular, like
the hippocampus (used in learning and memory functions and the olfactory epithelium (used for
smelling), do exhibit frequent birth and death of new neurons.
Myth 3: “The brain cannot repair itself.”

If neurons aren’t being replaced in adulthood, then how do people spontaneously recover from
neurological injuries like a stroke? One of the most amazing features of the brain is the phenomenon
of plasticity, the ability to change over time. Even if critical brain areas are damaged, it is theorized that
the brain learns how to “rewire itself”, essentially figuring out how to carry out these functions without
using the damaged connections. Unfortunately, there are some conditions that are neurodegenerative,
meaning that their symptoms get progressively worse over time. Many of these disorders, like
Parkinson’s disease and Alzheimer’s disease, currently do not have any simple cures or treatments
that don’t carry risks and side effects. For people with these conditions, there is not strong evidence
that the brain can recover from the destruction caused by these diseases.
Myth 4: “If you are analytical, you are left brain

dominant, but if you are creative, you are right brain

dominant.”
A common misconception is that the two hemispheres of the brain are responsible for wildly different
functions. The truth is that nearly every function that the left half of the brain can do, the right half
can do just as well, and vice versa. Sensory information, voluntary control of the muscles, memories,
and many other behaviors can be performed equally well by both the left and right halves of the brain.
A major exception to the “left vs. right” component is the processing and production of language. For
some reason unknown to scientists, these functions are heavily lateralized in the left hemisphere for
most people.
Fascinatingly, we do have one strange quirk about signaling between the brain and the rest of the
body: signaling pathways from the left brain crosses over to communicate with the right half of the
body, and vice versa. This contralateral organization is an unintended consequence of evolution, and is
one of the major distinguishing features of the vertebrate brain.
Neuroscience is ever-changing
One of the most exciting and satisfying aspects of modern science is the rapidity of new discoveries in
the field. New findings are often communicated by publishing academic studies in scientific journals.
More neuroscience studies were published between 2015 and 2020 than in the previous seventy years!
But, advancements in neuroscience haven’t always moved so quickly.
Trepanation was a surgical intervention that involved drilling a hole into an individual’s skull. It is
believed to be one of the oldest surgical procedures according to archaeological evidence. Interestingly,
skulls that show evidence of trepanation have been dated to 6500 BCE and show evidence of healing,
indicating that the patient survived the surgery.
Figure 1.7
Trepanated Skull.
This image
shows an
example of
trepanation, or
drilling holes in
the skull of
individuals as a
form of
treatment. The
growth of the
skull around the
site of surgery
suggests healing
and that this
procedure was
performed while
the individual
was still alive.
Localizationism
For hundreds of years, physicians attempted to correlate behaviors with changes in the brain. In the
mid 1800s, the physician Paul Broca contributed to localization theory by concluding that specific
areas of the brain were responsible for carrying out specific functions. This idea was supported by
ablation studies that demonstrated that when different brain structures were ablated, or lesioned,
there were specific associated functional losses. Further, electrically exciting specific brain structures
resulted in eliciting specific behaviors.
Most likely, some behaviors are more localized than others, but still rely on signals from across many
other brain areas. As with most fields of biology, absolutes are rare in neuroscience.
Figure 1.8. Image of Paul Broca.
Plasticity
The real strength of our brain is its flexibility: brains are capable of changing and adapting to a wide
variety of circumstances. Blind people use their visual areas of the brain while echolocating, stroke
survivors can regain lost motor functions using the unaffected brain circuits, and babies can effortlessly
learn two languages simultaneously in a bilingual household.
Plasticity is based on the idea that not only is the brain capable change, but that our experiences
change the structure and function of our nervous system.
Figure 1.9
Example of Brain
Plasticity. Brain
images are
shown of two
different
individuals, a
blind individual
that is an
echolocation
expert and a
control
participant. The
blind
echolocation
expert shows an
increase in
activity within
areas of the brain
that typically
respond to visual
information, but
no activation in
areas of the brain
that respond to
auditory
information. The
brain is plastic
and has allowed
for the blind
individual to use
areas of the brain
that typically
process sight to
instead process
echolocation
information.
Key Takeaways
• Neuroscience is the study of the nervous system and is an integrative field of study
that incorporates biology, psychology, chemistry, physics, mathematical modeling,
and health care providers.
• The study of neuroscience is accomplished through experimental studies, case
studies, and the use of experimental animal models.
• There are many popular myths concerning neuroscience and it is important to
analyze data that refutes these myths.
• Though the field of neuroscience is relatively young and ever-changing, humans
have been interested in the brain and its function for centuries.
Test Yourself!
An interactive H5P element has been excluded from this version of the text. You can
view it online here:
https://openbooks.lib.msu.edu/introneuroscience1/?p=407#h5p-23
Attributions
Portions of this chapter were remixed and revised from the following sources:
1. Open Neuroscience Initiative by Austin Lim. The original work is licensed under a Creative
Commons Attribution-NonCommercial 4.0 International License.
Media Attributions
• Comparison of Brain Size Across Species © OpenStax adapted by Valerie Hedges is licensed
under a CC BY-SA (Attribution ShareAlike) license
• Neuroscience is an integrative field of study © T. Wesley Mills adapted by Valerie Hedges is
licensed under a Public Domain license
• Scientific method © Efbrazil adapted by Valerie Hedges is licensed under a CC BY-SA
(Attribution ShareAlike) license
• Phineas Gage and his injury adapted by Valerie Hedges is licensed under a Public Domain license
• Commonly used animal models in neuroscience adapted by Valerie Hedges
• Preparations © Valerie Hedges is licensed under a CC BY-SA (Attribution ShareAlike) license
• Trepanated skull © Rama adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Paul Broca © Unknown is licensed under a Public Domain license
• Plasticity © Alan Thistle adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
CELLS OF THE NERVOUS SYSTEM STRUCTURE & FUNCTION | 23
PART II
CELLS OF THE NERVOUS
SYSTEM STRUCTURE &
FUNCTION
24 | CELLS OF THE NERVOUS SYSTEM STRUCTURE & FUNCTION
CELLS OF THE NERVOUS SYSTEM: THE NEURON | 25
2.
CELLS OF THE NERVOUS SYSTEM: THE

NEURON
There are 2 major cell types within the nervous system:

Neurons and Neuroglia. Neurons are cells that transmit
electrical information. Neuroglia are supporting cells of Resources
the nervous system.
Neurons are the basic units of the brain. Their main
function is to send electrical signals over short and long
• Scientist Links to Learn
distances in the body, and they are electrically and
More
chemically excitable. The function of the neuron is
• Glossary Terms
dependent on the structure of the neuron. The typical
• Key Takeaways
neuron consists of the dendrites, cell body, axon
• Test Yourself
(including the axon hillock), and presynaptic terminal.
26 | CELLS OF THE NERVOUS SYSTEM: THE NEURON
Figure 2.1. A typical neuron. Dendrites branch out from the cell body, where the nucleus is located.
The axon hillock is located where the cell body transitions into the axon. The axon begins at the
axon hillock and ends at the presynaptic terminal, which can branch into multiple terminals. ‘Neuron’
by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike
(CC-BY-NC-SA) 4.0 International License.
Although neurons do have a variety of adaptations that make them unique from other types of cells
in the body, they are still cells. Therefore, they contain all of the basic features of a typical mammalian
cell.
For example, they are made up of an aqueous cytoplasm bounded by a cell membrane. This cell
membrane, also called a plasma membrane or lipid membrane, consists of a sheet of several individual
molecules called phospholipids, which consist of two hydrophobic (water-fearing) tails and a
hydrophilic (water-loving) end. These phospholipids arrange themselves into a bilayer, with the
hydrophobic tails touching each other and the hydrophilic sides facing the cytoplasm and the
extracellular space, which are both mostly water. Because of the chemical properties of the cell
membrane, it is very effective at keeping ions and charged molecules separated, while allowing small
molecules like water and oxygen across the cell.
Neurons also have all the organelles that you would see in other cell types, like a nucleus and
mitochondria. The number of neurons in the adult human brain, according to our current best
estimate, is close to 86 billion. This number was calculated using a revolutionary technique, the
isotropic fractionator or “brain soup”, developed by Brazilian neuroanatomist Suzana Herculano-
Houzel. To put this number in context, we have about 37 trillion cells in the whole body, so neurons
in the brain make up about 0.2% of all cells in the body. Below are some unique characteristics that
neurons have in common.
1. Neurons are electroactive, which means that they are charged cells that can change their charge.
2. Neurons are specialized for rapid communication.
Many cells are capable of sending and receiving chemical signals across long distances and
time scales, but neurons are able to communicate with a combination of electrical and chemical
signals in a matter of milliseconds. Additionally, the shape of neurons and the organization of
the neurons on a microscopic level make them effective for sending signals in a very specific
direction.
3. Neurons are “forever” cells.
We are constantly replacing non-neuronal cells. For example, the cells in our bones replace
themselves frequently at a rate of about 10% each year. Our body makes new skin cells to replace
the dying skin cells on the surface so that we have a “new” skin every month. The cells along the
inside of our stomachs, exposed to very harsh acidic conditions, get replaced about every week.
About 100 million new red blood cells are created every minute! On the other hand, the mature
nervous system generally does not undergo much neurogenesis: the creation of new neurons.
The neurons that we have after development are the ones that we will keep until we die and
this permanence of neuronal count makes them different from almost every other cell of the
body. However, the idea of adult neurogenesis is a topic of debate among neuroscientists since
some areas, like the olfactory system and the hippocampus, display new nerve cell production.
4. …But, neurons can change.
Even though new neurons are not created in most areas of the brain, neurons still have the
capability to change in their structure and function. Some of these changes, such as physical
changes to the structures of the input sites of the neurons, are believed to last for a lifetime.
We use the word plasticity to describe the ability for the brain to alter its morphology. This
term is derived from the Greek plastikos, meaning “capable of being shaped or molded”—think
of plastic surgery, where a person changes their physical appearance.
Also, neurons do have the capacity to repair themselves to some extent. Neurons of the
Peripheral Nervous System may get injured or completely destroyed as a result of trauma to the
body. Afterwards, those injured neurons can regrow to connect once again with their original
partner. This regrowth seems to depend on a few chemical signals that the body produces, such
as nerve growth factor and brain derived neurotrophic factor. However, this process is often very
slow, and does not always successfully restore the nervous system to the way it was pre-injury.
Dendrites
The main function of neurons is to use changes in electrical properties in order to communicate with
connected cells. This communication usually moves in one direction, and we will use this pathway as
an outline for discussing the anatomical structures of the neurons.
Dendrites, shown here in green, are processes that branch out in a tree-like fashion from the cell
body. They are the main target for incoming signals received from other cells. The number of inputs
a neuron receives depends on the complexity of the dendritic branching. Dendrites may also have
small protrusions along the branches known as spines. Spines (illustrated in the inset box) are the sites
of some synaptic contacts. Spines increase the surface area of the dendritic arbor, which may be an
important factor in receiving communication.
We believe that spines are one of the most important sites where the nervous system is able to
change. For example, neurons change shape after exposure to various environmental conditions, such
as stress or exposure to drugs. Tiny changes to the surface of the neuron at the level of dendritic spines
is an example of plasticity.
Dendritic plasticity is thought to underlie the reason that we can learn new facts or maintain
memories about our childhood over long periods of time. Some set of tiny, submicroscopic changes to
the morphology of dendritic spines may represent a single complex memory that you form. A neuron
does not need spines for receiving information or for plasticity to take place. Many cells lack spines but
are still capable of permanently changing. The input site may be anywhere along the dendrite, or even
at the cell body—the “center” of the neuron.
Figure 2.2. Dendrites branch out from the soma. Their function is to receive information from other
neurons. Some dendrites have small protrusions called spines that are important for communicating
with other neurons. ‘Dendrites’ by Casey Henley is licensed under a Creative Commons Attribution
Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License.
Cell Body
Information that arrives through the many dendrites of a neuron eventually filters into the cell body,
or the soma, of the neuron. The cell body (shown below in green) contains the nucleus and cellular
organelles, including the endoplasmic reticulum, Golgi apparatus, mitochondria, ribosomes, and
secretory vesicles. The nucleus houses the DNA of the cell, which is the template for all proteins
synthesized in the cell. The organelles (illustrated in the inset box) in the soma are responsible for
cellular mechanisms like protein synthesis, packaging of molecules, and cellular respiration.
The cell body is responsible for deciding whether to pass a signal onto the next cell. The cell
membrane of the soma performs a complex set of “cellular arithmetic” that weighs all of the incoming
signals: excitatory, inhibitory, and modulatory signals. After all of the calculations have been
performed, the membrane decides to send a signal, either a “yes” or “no” output, which travels down
the axon.
Figure 2.3. The cell body, or soma, of the neuron contains the nucleus and organelles that are
commonly found in other cell types and are important for basic cellular functions. These organelles
include mitochondria, endoplasmic reticulum, and Golgi apparatus. ‘Soma’ by Casey Henley is
licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0
International License.
Axon
The axon is the main output extension of the neuron. The axon (highlighted in green) is usually a long,
single process that begins at the axon hillock and extends out from the cell body. The axon hillock is
located where the cell body transitions into the axon. Axons can branch in order to communicate with
more than one target cell.
Several axons can bundle and travel together; these are nerves. Axons can be very long; the longest
axon in the human body is part of the sciatic nerve that runs from the posterior end of the spinal cord
down the leg to control the muscles of the big toe.
Figure 2.4. The axon is a long single projection that begins at the axon hillock, the region between
the cell body and the axon. The axon terminates at the presynaptic terminal. ‘Axon’ by Casey Henley
is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0
Action Potential
The axon transmits an electrical signal—called an action potential—from the axon hillock to the
presynaptic terminal, where the electrical signal will result in a release of chemical neurotransmitters
to communicate with the next cell. The action potential is a very brief change in the electrical potential,
which is the difference in charge between the inside and outside of the cell. During the action potential,
the electrical potential across the membrane moves from a negative value to a positive value and back.
One or more interactive elements has been excluded from this version of the text. You
can view them online here: https://openbooks.lib.msu.edu/
introneuroscience1/?p=29#video-29-1
Animation 2.1. The action potential is a brief but significant change in electrical potential across the
membrane. The membrane potential will move from a negative, resting membrane potential, shown
here as -65 mV, and will rapidly become positive and then rapidly return to rest during an action
potential. The action potential moves down the axon beginning at the axon hillock. When it reaches
the synaptic terminal, it causes the release of chemical neurotransmitter. ‘Action Potential
Propagation’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Share-Alike (CC-BY-NC-SA) 4.0 International License. View static image of animation.
Myelin
Many axons are also covered by a myelin sheath, a fatty substance that wraps around portions of
the axon and increases action potential speed. There are breaks between the myelin segments called
Nodes of Ranvier, and this uncovered region of the membrane regenerates the action potential as
it propagates down the axon in a process called saltatory conduction. There is a high concentration
of voltage-gated ion channels, which are necessary for the action potential to occur, in the Nodes of
Ranvier.
Figure 2.5. Myelin wraps around and insulates the axon. The spaces between the myelin sheath,
where the axon is uncovered, are call the Nodes of Ranvier. ‘Myelin’ by Casey Henley is licensed
under a Creative Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0
Axon Characteristics
Axon Length
The length of an axon is variable depending on the location of the neuron and its function. The axon
of a sensory neuron in your big toe needs to travel from your foot up to your spinal cord, whereas an
interneuron in your spinal cord may only be a few hundred micrometers in length.
Figure 2.6. Axons vary in length. Spinal interneurons, neurons that fully exist within the spinal cord,
can have short axons, whereas sensory or motor neurons, which need to reach from the spinal cord
to the appropriate body region, for example the toe, have long axons. ‘Axon Length’ by Casey
Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike
Axon Diameter
Axon diameter is also variable and can be used to differentiate different types of neurons. The
diameter affects the speed at which the action potential will propagate. The larger the diameter, the
faster the signal can travel. Additionally, larger diameter axons tend to have thicker myelin.
Figure 2.7. The diameter of the axon and the amount of myelination varies. Large diameter axons
typically have thicker myelin sheath, which results in fast action potential speed. Small diameter
axons may have no myelin present, resulting in slow action potential speed. ‘Axon Diameter’ by
Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike
Axoplasmic Transport
Axoplasmic transport refers to the movement of material within the axon. Organelles, vesicles, and
proteins can be moved from the cell body to the terminal via anterograde transport or from the
terminal to the cell body via retrograde transport. Anterograde transport can be either fast or slow.
Microtubules run the length of the axon and provide the cytoskeleton tracks necessary for the
transportation of materials. Proteins aid in axoplasmic transport. Kinesin is a motor protein that uses
ATP and is used in anterograde transport of materials. Dynein is another motor protein that also uses
ATP, but is used in retrograde transport of materials.
The Synapse
Figure 2.8. Cellular components need to be able to
move throughout the cell to have proper
functioning. Anterograde transport moves The synapse is the physical distance that
components from the cell body toward the
separates two neurons.
terminal. Retrograde transport moves
components from the terminal toward the cell
body. ’Axonal Transport’ by Casey Henley is
licensed under a Creative Commons Attribution Electrical Synapse
Non-Commercial Share-Alike (CC BY-NC-SA) 4.0
Electrical synapses physically share cytoplasm.
An electrical synapse may be less than 5
nanometers apart. Cells connected by electrical synapses share cytoplasm, but have two separate cell
membranes.
Figure 2.9. Since

an electrical
synapse is a
direct, physical
connection
between the
cytoplasm of two
neurons, ions are
able to flow in
either direction
across the gap
junction.
‘Bidirectional
Electrical
Synapse’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Chemical Synapse
Chemical synapses use neurotransmitters to communicate. Chemical synapses can vary depending
on the nature of the synapse. A chemical synapse is a larger distance, about 15–40 nm across. Adjacent
neurons connected by chemical synapses do not share cytoplasm.
Figure 2.10. Synapses are found between two adjacent cells. In this image, the axon terminals
(presynaptic terminals) are synapsing on the dendrites of another neuron (postsynaptic cell).
“Presynaptic Terminal’ by Casey Henley is licensed under a Creative Commons Attribution
Presynaptic versus Postsynaptic

The axon terminates at the presynaptic terminal or terminal bouton. The terminal of the presynaptic
cell forms a synapse with another neuron or cell, known as the postsynaptic cell. When the action
potential reaches the presynaptic terminal, the neuron releases neurotransmitters into the synapse. The
neurotransmitters act on the postsynaptic cell. Therefore, neuronal communication requires both an
electrical signal (the action potential) and a chemical signal (the neurotransmitter). Most commonly,
presynaptic terminals contact dendrites, but terminals can also communicate with cell bodies or even
axons. Neurons can also synapse on non-neuronal cells such as muscle cells or glands.
Figure 2.11. The presynaptic terminal forms synaptic contacts with a postsynaptic cell. ‘Presynaptic
Terminal’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Share-Alike (CC-BY-NC-SA) 4.0 International License.
The terms presynaptic and postsynaptic are in reference to which neuron is releasing
neurotransmitters and which is receiving them. Presynaptic cells release neurotransmitters into the
synapse and those neurotransmitters act on the postsynaptic cell.
Figure 2.12. The presynaptic cell is the neuron that releases neurotransmitters into the synapse to
act upon the postsynaptic cell. ‘Postsynaptic Cell’ by Casey Henley is licensed under a Creative
Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License.
Variations in Structure
Although these typical structural components can be seen in all neurons, the overall structure can vary
drastically depending on the location and function of the neuron. Some neurons, called unipolar, have
only one branch from the cell body, and the dendrites and axon terminals project from it. Others,
called bipolar, have one axonal branch and one dendritic branch. Multipolar neurons can have many
processes branching from the cell body. Additionally, each of the projections can take many forms,
with different branching characteristics. The common features of cell body, dendrites, and axon,
though, are common among all neurons.
Figure 2.13. Neuron structure is variable, but the main components of cell body (shown in black),
dendrites (shown in brown), and axon (shown in blue) are common among all neurons. ‘Neuron
Types‘ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Key Takeaways
• Each structural component of the neuron has an important function

• Overall structure of the cell can vary depending on location and function of
the neuron
Test Yourself!
Attributions
1. Foundations of Neuroscience by Casey Henley. The original work is licensed under a Creative
Commons Attribution-NonCommercial-ShareAlike 4.0 International License
CELLS OF THE NERVOUS SYSTEM: GLIA | 43
3.
CELLS OF THE NERVOUS SYSTEM: GLIA
Although most of neuroscience is concerned with

understanding the functions of neurons, there are other
cells in the nervous system that are just as interesting.
These cells are grouped together under the umbrella Resources
classification of glia. Historically, when these non-
neuronal cells were visualized under the microscope, the
histologists and anatomists had no idea about their
function. They were seen all around the neurons, so the • Glossary Terms
assumption was that these cells were structural elements, a • Key Takeaways
sort of living glue, that held the nervous system together. • Test Yourself
Today, we know that these glia serve a variety of functions;
unfortunately, the misnomer “glia”—derived from the
Latin word for “glue”—is still used to describe these non-
neuronal components of the nervous system.
Astrocytes
Astrocytes are named for their characteristic star-shaped morphology. One of the main functions of
astrocytes in the brain is to help maintain the blood-brain barrier. At the end of the extensions of the
astrocyte are protrusions called “endfeet”. These endfeet are often wrapped around the endothelial cells
that surround the blood vessels. The endfeet release important biological compounds that allow the
endothelial cells to remain healthy as they function in maintaining the blood-brain barrier. Astrocytes
are also very closely associated with synapses.
Astrocytes also synthesize and produce a variety of trophic factors, which are helper molecular
signals that serve several different functions. For one, trophic factors signal to neurons that the neuron
44 | CELLS OF THE NERVOUS SYSTEM: GLIA
should continue to live, or that specific synapses should be maintained. They help guide the neurons
as they reach out, forming synapses where appropriate.
Figure 3.1
Astrocyte. A
green fluorescent
marker has been
used to stain
astrocytes within
brain tissue. The
astrocytes has
star-like
projections off
the cell body.
Oligodendrocytes
The main function of the oligodendrocytes is to add a layer of myelin around the axons of nearby
neurons in the central nervous system. A single oligodendrocyte is able to myelinate up to 50
segments of axons. As cells that produce myelin, they are responsible for increasing the conduction
speed of nearby neurons as they send signals. Oligodendrocytes only exist in the central nervous
system.
Figure 3.2 Image

of an
oligodendrocyte.
A single
oligodendrocyte
shown in blue
covers axons
from multiple
neuron axons
with myelin
sheath.
Schwann Cells
Schwann cells can only be found in the peripheral nervous system. The main action of Schwann
cells is to provide a section of myelin sheath for peripheral nervous system neurons, and in this way,
they function similarly to the oligodendrocytes. Schwann cells produce only a single section of myelin,
compared to oligodendrocytes, which myelinate multiple sections. Schwann cells also function in the
regeneration of injured axons. When nerves in the peripheral nervous system are damaged after trauma,
Schwann cells rapidly mobilize to the site of injury.
Figure 3.3
Schwann Cell.
The Schwann cell
is shown in blue.
A single
Schwann cell
wraps myelin
around a neuron
axon, shown in
yellow. Multiple
Schwann cells
are needed to
myelinate one
neuron axon.
Microglia
Microglia are a bit different from the other glial cell populations. For one, microglia are more immune
cells rather than neural. They act as cellular scavengers that travel throughout the brain and spinal cord.
It is estimated that microglia make up 10-15% of all cells in the brain.
As immune cells, microglia identify and destroy clumps of proteins, dead/dying cells, or foreign
pathogens that enter into the brain. After an injury to the central nervous system, like a traumatic
blow to the head, microglia rapidly react to the area of the insult.
Figure 3.4
Photograph of
microglia and
neurons. In this
microscope
photograph,
microglia are
stained with a
green fluorescent
stain and
neurons are
stained with a
red fluorescent
stain. Microglia
are much smaller
than neurons.
Ependymal Cells
Along the inside of the ventricles are a lining of glia called ependymal cells. These ependymal cells
are columnar with small fingerlike extensions called cilia that extend into the ventricles and into the
central canal that runs down the inside of the spinal cord. The cilia have motor properties that allow
for them to rhythmically beat to create a current in the surrounding fluid.
Figure 3.5 Image

of brain
ventricles. The
brain ventricles
(shown in blue)
are hollow areas
within the brain
that are
interconnected
and filled with
cerebrospinal
fluid. The
ventricles are
connected to the
central canal of
the spinal cord.
The ventricles are
show in a lateral
view (left) and
anterior view
(right).
Ependymal cells produce cerebral spinal fluid (CSF). In total, the body can make about half a liter
of CSF each day (a little more than two cups.) The ependymal cells are part of a structure called the
choroid plexus, the network of blood vessels and cells that form a boundary between the blood and the
CSF.
Figure 3.6.
Ependymal Cells.
Ependymal cells
are ciliated
columnar cells
that line the
ventricles and
other fluid-filled
spaces of the
central nervous
system. The
rhythmic beating
of the cilia create
movement of the
surrounding
cerebral spinal
fluid. ‘Ependymal
Cells’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Key Takeaways
• There are multiple different types of glia cells that each have their own functions
Test Yourself!
Attributions
Media Attributions
• Astrocyte © Bruno Pascal adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Oligodendrocyte © Holly Fischer adapted by Valerie Hedges is licensed under a CC BY
(Attribution) license
• Schwann cell © OpenStax adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Microglia © Gerry Shaw adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Brain Ventricles © Bruce Blaus adapted by Valerie Hedges is licensed under a CC BY
• Ependymal Cells © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
NonCommercial ShareAlike) license
50 | VISUALIZING CELLS OF THE NERVOUS SYSTEM
4.
VISUALIZING CELLS OF THE NERVOUS

SYSTEM
For the majority of human history, the only way we were

able to study the structure of the brain was with crude,
butcher-like methods. Wait for a person to die, saw a giant
Resources hole in the top of the skull, take the brain out, and slice it
into pieces to see if there was some correlation between the
way the brain looks and the way they died. With these
methods, only major changes in gross anatomy could be
• Scientist Links to Learn observed, such as those resulting from severe birth defects
More or trauma.
• Glossary Terms Brain analysis methods were enhanced by the scientific
• Key Takeaways adoption of light microscopy. Naturalists in the mid
• Test Yourself 1600s such as Antonie van Leeuwenhoek, Jan
Swammerdam, and Robert Hooke began looking at
biological substances up close, and the brain proved to be
a complex and interesting sample of tissue.
Visualizing Cells: Cell Staining

Staining is an imaging method that is often used in conjunction with microscopy. Thin slices of brain
tissue are exposed to various chemical processes. The chemicals that are used for staining have different
affinities for parts of cells.
For staining to work, the tissue needs to be subjected to a series of chemical processes. First, the
tissue needs to be fixed. Fixation is a chemical process that is accomplished by exposing the tissue
to a chemical like paraformaldehyde. The most effective way to expose every part of the body to
VISUALIZING CELLS OF THE NERVOUS SYSTEM | 51
fixative is to “hijack” the endogenous circulatory system by flushing fixative through the arteries, a
process called perfusion. Chemically, fixatives cause adjacent proteins to become covalently bonded
(crosslinked), a process which causes the proteins to become unchangeable; they become “fixed” in
time. These fixatives are very harsh chemicals, and usually kill microorganisms and inactivate the
endogenous enzymes that normally degrade biological tissue. (As a side note, fixatives are particularly
nasty carcinogens that can permeate easily through latex gloves.)
After fixation, devices such as a microtome or cryostat are used to slice the brain into sections as thin
as 10 microns. Stains do not always reliably pass all the way through thick sections of tissue, such as an
intact brain. With thin slices, chemical stains are able to permeate through the depth of the tissue.
Next, stains are used to color the cells of the nervous system. This process is used because it is good
for identifying the location of specific proteins at a subcellular level.
Nissl-Staining (Cresyl Violet Staining)

A stain is needed to distinguish individual cells in nervous tissue.
Nissl stain (Cresyl Violet Stain) was discovered by Franz Nissl. It stains nucleic acids such as RNA
and DNA. Thus, the stain is only localized to neuronal cell bodies, where RNA and DNA are found.
This staining method is useful for studying neuronal arrangement and how densely neurons are packed
in specific brain structures.
Figure 4.1 Nissl

Staining (Cresyl
Violet Staining).
This microscope
photograph
shows neuronal
cell bodies
stained purple
within mouse
brain tissue in a
structure called
the
hippocampus.
The Nissl stain
allows
researchers to
determine
cellular density.
Golgi Staining
A major advancement in the study of neuronal morphology came about in the late 1800s. The Italian
anatomist and biologist Camillo Golgi identified a shortcoming with the cellular analysis techniques
of the time: structures in the central nervous sytem were impossible to distinguish from one another.
The cells in the brain were so densely packed together, that it became difficult to identify which cellular
material belonged to which cell.
Figure 4.2
Photograph
image of Camillo
Golgi, the
developer of the
Golgi-staining
technique.
Golgi came up with a new technique using a silver compound that caused the silver to precipitate
inside the cell membranes. However, not every cell took up the silver. Instead, only a small fraction
of neurons, maybe 1% or even less, were completely stained in black, which stood out remarkably
well against the light yellow background of the surrounding tissue. This reaction, initially called the
“black reaction”, is now known as a “Golgi stain”. (Despite being more than a hundred years old, we
currently don’t know the mechanism by which the silver stain is taken up into the neurons, or what
determines why certain cells take the stain and others don’t.)
Because of the great contrast between cell and background, every single part of the neuron was
completely filled, allowing Golgi to do drawings of the morphology of this nervous tissue. Based on
his staining results, Golgi supported the idea that the parts of the nervous system are all one very large,
physically connected network. This idea was known as the Reticular Theory.
Figure 4.3
Golgi-stained
pyramidal
neuron. In this
microscope
photograph, the
black/dark stain
is staining a
single neuron
and its
projections. The
Golgi stain allows
for visualization
of dendrites and
the neuron axon.
Santiago Ramon y Cajal

About 10 years later, the Spanish neuroanatomist Santiago Ramon y Cajal repeated some of Golgi’s
staining experiments with other sections of nervous tissue. Looking at similar darkly-filled neurons,
Cajal arrived at a different conclusion: the nervous system is not a giant net, but rather a series of
individual units that are separated from one another physically. This idea came to be known as the
Neuron Doctrine.
Figure 4.4.
Photograph of
Santiago Ramon
y Cajal.
Both Golgi and Cajal were awarded the shared Nobel Prize in Physiology or Medicine in 1906 for
their accomplishments in helping to understand “the structure of the nervous system”. Even though
Cajal’s Neuron Doctrine was adopted widely by scientists, the elucidation of this organization would
not have been made possible without Golgi’s development of the silver stain. The sharing of this
prestigious award was ironic because of the many disagreements between the two scientists.
Cajal’s Neuron Doctrine was eventually given more support with the aid of modern techniques,
like electron microscopy, that are capable of physically seeing the distance between two neurons. The
Neuron Doctrine represents our current understanding of how the nervous system is organized.
Figure 4.5.
Example of
Santiago Ramon
y Cajal drawing.
This is an
example of one
of the many
intricate hand
drawings done
by Santiago
Ramon y Cajal
while he looked
through a
microscope at
nervous system
tissue.
Key Takeaways
• Nissl stains stain neuronal cell bodies

• Golgi stains stain entire neurons and allows for visualization of cell structures
(dendrites and axons)
• Neurons are discreet cells and are not interconnected together together into a
network
Test Yourself!
Attributions
Media Attributions
• Nissl Stain © Marika Nosten-Bertrand, Caroline Kappeler, Céline Dinocourt, Cécile Denis,
Johanne Germain, Françoise Phan Dinh Tuy, Soraya Verstraeten, Chantal Alvarez, Christine
Métin, Jamel Chelly, Bruno Giros, Richard Miles, Antoine Depaulis et Fiona Francis adapted by
Valerie Hedges is licensed under a CC BY (Attribution) license
• Camillo Golgi © Unknown adapted by Valerie Hedges is licensed under a CC BY (Attribution)
license
• Golgi Stain © Cahass adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Santiago Ramon y Cajal © Anonymous adapted by Valerie Hedges is licensed under a Public
Domain license
• Santiago Ramon y Cajal drawing © Santiago Ramon y Cajal adapted by Valerie Hedges is
56 | ION MOVEMENT
5.
ION MOVEMENT
Ion flow into and out of the neuron is a critical

component of neuron function. Ions move in predictable
Resources ways, and the control of ion movement affects the cell at
rest and while sending and receiving information from
other neurons.
• Glossary Terms
• Key Takeaways Phospholipid Bilayer
• Test Yourself
Prevents Ion Movement
The cell membrane that separates the inside of the cell
from the outside is a very effective boundary. It is described as being selectively permeable, which means
that some molecules are able to travel across the membrane easily, other molecules have an intermediate
ability to cross, and other molecules are completely incapable of passing. Generally, gases and molecules
of water are able to pass through the cell membrane easily. Large molecules like glucose, and charged
molecules like ions or amino acids, are unable to pass across the membrane.
The neuronal membrane is composed of lipid molecules that form two layers. The hydrophilic
heads of the molecules align on the outside of the membrane, interacting with the intra- and
extracellular solution of the cell, whereas the hydrophobic tails are arranged in the middle, forming a
barrier to water and water-soluble molecules like ions. This barrier is critical to neuron function.
ION MOVEMENT | 57
Figure 5.1. The neuronal membrane is composed of two layers of phospholipid molecules that form a
barrier to water and water-soluble molecules due to the organization of the hydrophilic heads and
hydrophobic ends of the molecules. ‘Phospholipid Bilayer’ by Casey Henley is licensed under a
Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License.
Ion Channels Allow Ion Movement

Most cells of the body, including neurons, have specialized transmembrane proteins embedded in the
cell membrane. These transmembrane proteins are huge protein complexes that span the entirety of
the membrane, with an outer side and an inner side. In the middle of the protein is a pore, which is
essentially a “tunnel” that allows molecules and ions to pass across the cell membrane. These proteins
are called ion channels. These channels are passive since they do not use any cellular energy to move
ions. Rather, they simply provide easy passage for ions. It may be useful to think of an ion channel as a
“cellular door”. Ion channels are embedded throughout the neuronal membrane.
Channels can be opened and closed in a number of different ways. We can categorize ion channels
into four major classes based on their opening and closing conditions.
1. Leak channels are persistently open. You can think of these leak channels as revolving doors
that are never locked. Neurons usually have several leak channels.
2. Voltage-gated ion channels open in response to a change in membrane potential.
3. Ligand-gated ion channels open in response to chemical (ligand) binding, such as
neurotransmitters.
4. The fourth class of ion channels is a catch-all category that includes a wide variety of
channels that are used by the sensory systems. They open and close in response to unique
stimuli depending on what they are able to sense. For example, some open and close depending
when they are moved physically, such as a distortion or stretch (mechanoreceptors). We have
these in the hair cells of our ears, in our skin, and in our muscles. Photoreceptors in our eyes
58 | ION MOVEMENT
have ion channels that close in response to being hit by photons of light, and this activity is
necessary for us to be able to see in both brightly and dimly lit environments.
One important feature of ion channels is their ability to distinguish ions based on their chemical
properties. For example, some channels are selective for Na+, while preventing the passage of all other
ions. Each ion channel has special molecular characteristics that allow certain types of ions to pass
through the pore while excluding other ions. Channels can be specific to one ion or allow the flow of
multiple ions.
Figure 5.2. The phospholipid bilayer with embedded ion channels. The dotted, blue channels
represent sodium channels; the striped, green channels represent potassium channels; the solid
yellow channels represent chloride channels. ‘Membrane with Channels’ by Casey Henley is licensed
under a Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License.
Ion channels control ion movement across the cell membrane because the phospholipid bilayer is
impermeable to the charged atoms. When the channels are closed, no ions can move into or out of the
cell. When ion channels open, however, then ions can move across the cell membrane.
Animation 5.1. When ion channels in the membrane are closed, ions cannot move into or out of the
neuron. Ions can only cross the cell membrane when the appropriate channel is open. For example,
only sodium can pass through open sodium channels. The dotted, blue channels represent sodium
channels; the striped, green channels represent potassium channels; the solid yellow channels
represent chloride channels. ‘Ion Movement’ by Casey Henley is licensed under a Creative Commons
ION MOVEMENT | 59
Attribution Non-Commercial (CC-BY-NC) 4.0 International License. View static image of

animation.
Gradients Drive Ion Movement

Ions move in predictable ways. Concentration (chemical) and electrical gradients drive ion movement.
The chemical gradient refers to the natural process by which a high concentration of a substance,
given enough time, will eventually diffuse to a lower concentration and settle evenly over the space.
Ions will diffuse from regions of high concentration to regions of low concentration. Diffusion is a
passive process, meaning it does not require energy. As long as a pathway exists (like through open ion
channels), the ions will move down the concentration gradient.
In addition to concentration gradients, electrical gradients can also drive ion movement. The
electrical gradient refers to the electrical forces acting on charged molecules, “pulling” opposite
charges together while also “pushing” like charges away from one another—just like the polarity of
magnets. Ions are attracted to, and will move toward, regions of opposite charge. Positive ions will
move toward regions of negative charge, and vice versa.
For discussion of ion movement in this text, the combination of these two gradients will be referred
to as the electrochemical gradient. Sometimes the concentration and electrical gradients driving ion
movement can be in the same direction; sometimes the direction is opposite. The electrochemical
gradient is the summation of the two individual gradients and provides a single direction for ion
movement.
Animation 5.2. Concentration and electrical gradients drive ion movement. Ions diffuse down
concentration gradients from regions of high concentration to regions of low concentration. Ions also
move toward regions of opposite electrical charge. ‘Gradients’ by Casey Henley is licensed under a
Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License. View
60 | ION MOVEMENT
static image of animation.
When Gradients Balance, Equilibrium Occurs

When the concentration and electrical gradients for a given ion balance—meaning they are equal
in strength, but in different directions—that ion will be at equilibrium. Ions still move across the
membrane through open channels when at equilibrium, but there is no net movement in either
direction, meaning there is an equal number of ions moving into the cell as there are moving out of the
cell.
Animation 5.3. When an ion is at equilibrium, which occurs when the concentration and electrical
gradients acting on the ion balance, there is no net movement of the ion. The ions continue to move
across the membrane through open channels, but the ion flow into and out of the cell is equal. In this
animation, the membrane starts and ends with seven positive ions on each side even though the ions
move through the open channels. ‘Ion Equilibrium’ by Casey Henley is licensed under a Creative
Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License. View static image
of animation.
Important Ions for Neurons

Sodium, potassium, and chloride ions are found in different concentrations across the neuron cell
membrane. The location of these ions across the cell membrane and their concentration gradients
are important for the function of the neuron. Sodium (Na+) ions are more concentrated in the
extracellular fluid and less concentrated within the intracellular fluid. Whereas potassium (K+) ions are
more concentrated within the intracellular fluid and less concentrated in the extracellular fluid.
ION MOVEMENT | 61
Table 5.1. Concentrations of sodium, potassium, and chloride inside and outside of the cell.
Ion Inside concentration (mM) Outside concentration (mM)
Sodium (Na+) 15 145
Potassium (K+) 125 5
Chloride (Cl-) 13 150
Key Takeaways
• The phospholipid bilayer prevents ion movement into or out of the cell
• Ion channels allow ion movement across the membrane
• Electrochemical gradients drive the direction of ion flow
• At equilibrium, there is no net ion movement (but ions are still moving)
Test Yourself!
Attributions
62 | ION MOVEMENT
MEMBRANE POTENTIAL | 63
6.
MEMBRANE POTENTIAL
The membrane potential is the difference in electrical

charge between the inside and the outside of the neuron.
This is measured using two electrodes. A reference Resources
electrode (also called the ground electrode) is placed in the
extracellular solution. The recording electrode is inserted
into the cell body of the neuron.
• Glossary Terms
• Key Takeaways
• Test Yourself
64 | MEMBRANE POTENTIAL
Figure 6.1. The membrane potential is measured using a reference electrode placed in the
extracellular solution and a recording electrode placed in the cell soma. The membrane potential is
the difference in voltage between these two regions. ‘Measuring Membrane Potential’ by Casey
Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC
BY-NC-SA) 4.0 International License.
Terminology
There is more than one way to describe a change in membrane potential. The membrane potential is
measured as the difference in charge between the outside of the cell and the inside of the cell. If the
membrane potential moves toward zero, that is a depolarization, because the membrane is becoming
less polarized—meaning there is a smaller difference between the charge on the inside of the cell
compared to the outside. This is also referred to as a decrease in membrane potential. This means that
when a neuron’s membrane potential moves from rest, which is typically around -65 mV, toward 0 mV
and becomes more positive, this is a decrease in membrane potential. Since the membrane potential is
the difference in electrical charge between the inside and outside of the cell, that difference decreases as
the cell’s membrane potential moves toward 0 mV.
If the membrane potential moves away from zero, or gets more negative than it was at rest, that is
a hyperpolarization because the membrane is becoming more polarized. This is also referred to as an
increase in membrane potential.
Figure 6.2. A decrease in membrane potential is a change that moves the cell’s membrane potential
toward 0, or depolarizes the membrane. An increase in membrane potential is a change that moves
the cell’s membrane potential away from 0 or hyperpolarizes the membrane. ‘Membrane Potential
Terms’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Share-Alike (CC BY-NC-SA) 4.0 International License.
Voltage Distribution
At rest, ions are not equally distributed across the membrane. This distribution of ions and other
charged molecules leads to the inside of the cell having a more negative charge compared to the outside
of the cell.
Figure 6.3. The inside of the neuron has a more negative charge than the outside of the neuron.
‘Membrane Potential’ by Casey Henley is licensed under a Creative Commons Attribution
Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License.
A closer look shows that sodium, calcium, and chloride are concentrated outside of the cell membrane
in the extracellular solution, whereas potassium and negatively-charged molecules like amino acids and
proteins are concentrated inside in the intracellular solution.
Figure 6.4. For a typical neuron at rest, sodium, chloride, and calcium are concentrated outside the
cell, whereas potassium and other anions are concentrated inside. This ion distribution leads to a
negative resting membrane potential. The dotted, blue channels represent sodium leak channels;
the striped, green channels represent potassium leak channels; the solid yellow channels represent
chloride leak channels. ‘Membrane at Rest’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License.
Ion Distribution Creates Electrochemical

Gradients
These concentration differences lead to varying degrees of electrochemical gradients in different
directions depending on the ion in question. For example, the electrochemical gradients will drive
potassium out of the cell, but will drive sodium into the cell.
Figure 6.5. The distribution of ions on either side of the membrane lead to electrochemical gradients
for sodium and potassium that drive ion flow in different directions. If the membrane is permeable
to sodium, ions will flow inward. If the membrane is permeable to potassium, ions will flow
outward. The dotted, blue channels represent sodium channels; the striped, green channels
represent potassium channels; the solid yellow channels represent chloride channels. ‘Gradients
Across Membrane’ by Casey Henley is licensed under a Creative Commons Attribution
Equilibrium Potential
The neuron’s membrane potential at which the electrical and concentration gradients for a given ion
balance out is called the ion’s equilibrium potential. Let’s look at sodium in more detail:
Example: Driving Forces on Sodium Ions

When sodium channels open, the neuron’s membrane becomes permeable to sodium, and
sodium will begin to flow across the membrane. The direction is dependent upon the
electrochemical gradients. The concentration of sodium in the extracellular solution is about
10 times higher than the intracellular solution, so there is a concentration gradient
driving sodium into the cell. Additionally, at rest, the inside of the neuron is more negative
than the outside, so there is also an electrical gradient driving sodium into the cell.
As sodium moves into the cell, though, these gradients change in driving strength. As the
neuron’s membrane potential become positive, the electrical gradient no longer works to
drive sodium into the cell. Eventually, the concentration gradient driving sodium into the
neuron and the electrical gradient driving sodium out of the neuron balance with equal and
opposite strengths, and sodium is at equilibrium. The membrane potential of the neuron at
which equilibrium occurs is called the equilibrium potential of an ion, which is
approximately +60 mV for sodium.
One or more interactive elements has been excluded from this version of the text. You can
view them online here: https://openbooks.lib.msu.edu/introneuroscience1/?p=41#video-41-1
Animation 6.1. At rest, both the concentration and electrical gradients for sodium point into
the cell. As a result, sodium flows in. As sodium enters, the membrane potential of the cell
decreases and becomes more positive. As the membrane potential changes, the electrical
gradient decreases in strength, and after the membrane potential passes 0 mV, the
electrical gradient will point outward, since the inside of the cell is more positively charged
than the outside. The ions will continue to flow into the cell until equilibrium is reached. An
ion will be at equilibrium when its concentration and electrical gradients are equal in
strength and opposite in direction. The membrane potential of the neuron at which this
occurs is the equilibrium potential for that ion. Sodium’s equilibrium potential is
approximately +60 mV. The dotted, blue channels represent sodium channels; the striped,
green channels represent potassium channels; the solid yellow channels represent chloride
channels. ‘Sodium Gradients’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License. View

Calculate Equilibrium Potential with Nernst Equation

The gradients acting on the ion will always drive the ion towards equilibrium. The equilibrium
potential of an ion is calculated using the Nernst equation:
The Nernst Equation
The constant, 61, is calculated using values such as the universal gas constant and
temperature of mammalian cells
Z is the charge of the ion
[Ion]inside is the intracellular concentration of the ion
[Ion]outside is the extracellular concentration of the ion
An Example: Sodium’s Equilibrium Potential

For Sodium:
z=1
[Ion]inside = 15 mM
[Ion]outside = 145 mM
Predict Ion Movement by Comparing Membrane

Potential to Equilibrium Potential
It is possible to predict which way an ion will move by comparing the ion’s equilibrium potential to
the neuron’s membrane potential. Let’s assume we have a cell with a resting membrane potential of
-70 mV. Sodium’s equilibrium potential is +60 mV. Therefore, to reach equilibrium, sodium will need
to enter the cell, bringing in positive charge. On the other hand, chloride’s equilibrium potential is -65
mV. Since chloride is a negative ion, it will need to leave the cell in order to make the cell’s membrane
potential more positive to move from -70 mV to -65 mV.
Figure 6.6. A) If a cell is at rest at -70 mV, sodium ions will flow into the cell to move the cell’s
membrane potential toward sodium’s equilibrium potential of +60 mV. B) At the same resting
membrane potential, chloride would flow out of the cell, taking away its negative charge, making
the inside of the cell more positive and moving toward chloride’s equilibrium potential of -65 mV.
The dotted, blue channels represent sodium channels; the striped, green channels represent
potassium channels; the solid yellow channels represent chloride channels. ‘Moving Toward
Equilibrium’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Concentration and Equilibrium Potential Values

We will use the following ion concentrations and equilibrium potentials:
Table 6.1. Typical ion concentrations inside and outside of the Neuron and the equilibrium potential for each ion.
Ion Inside concentration (mM) Outside concentration (mM) Equilibrium Potential
Sodium (Na+) 15 145 +60 mV
Potassium (K+) 125 5 -85 mV
Chloride (Cl-) 13 150 -65 mV

Key Takeaways
• Moving the membrane potential toward 0 mV is a decrease in potential; moving

away from 0 mV is an increase in potential
• The distribution of ions inside and outside of the cell at rest vary among the different
ions; some are concentrated inside, some are concentrated outside
• Equilibrium potentials are calculated using the Nernst equation
• To predict ion movement, compare the current membrane potential of the neuron
with the ion’s equilibrium potential. Determine which way the ion needs to move to
cause that membrane potential change (i.e. does the ion need to move into the cell or
out of the cell?)
Test Yourself!
Attributions

THE MEMBRANE AT REST | 77
7.
THE MEMBRANE AT REST
As covered in the previous chapter, at rest there is an

uneven distribution of ions on either side of the
membrane. The inside of the neuron is more negatively Resources
charged than the outside. The resting membrane potential
of a typical neuron is around -65mV to -70mV, though it
can vary.
• Glossary Terms
• Key Takeaways
• Test Yourself
78 | THE MEMBRANE AT REST
Figure 7.1. For a typical neuron at rest, sodium, chloride, and calcium are concentrated outside the
cell, whereas potassium and other anions are concentrated inside. This ion distribution leads to a
negative resting membrane potential. The dotted, blue channels represent sodium leak channels;
the striped, green channels represent potassium leak channels; the solid yellow channels represent
chloride leak channels. ‘Membrane at Rest’ by Casey Henley is licensed under a Creative Commons
Why is the Resting Membrane Potential -65 mV?

How the ions are distributed across the membrane plays an important role in the generation of the
resting membrane potential. When the cell is at rest, some non-gated, or leak, ion channels are actually
open. Significantly more potassium channels are open than sodium channels, and this makes the
membrane at rest more permeable to potassium than sodium.
Figure 7.2. At rest, the distribution of ions across the membrane varies for different ions.
Additionally, at rest, more potassium non-gated ion channels (emphasized by green circles) are open
than sodium channels (emphasized by the blue circle). The dotted, blue channels represent sodium
leak channels; the striped, green channels represent potassium leak channels; the solid yellow
channels represent chloride leak channels. ‘Channels at Rest’ by Casey Henley is licensed under a
Creative Commons Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International
License.
1. Potassium Can Cross Membrane at Rest through

Leak Channels
Since the membrane is permeable to potassium at rest due to the open non-gated channels, potassium
will be able to flow across the membrane. The electrochemical gradients at work will cause potassium
to flow out of the cell in order to move the cell’s membrane potential toward potassium’s equilibrium
potential of -80 mV.
Animation 7.1. Electrochemical gradients drive potassium out of the cell, removing positive charge,
making the cell’s membrane potential more negative, in the direction of potassium’s equilibrium
potential. The dotted, blue channels represent sodium leak channels; the striped, green channels
represent potassium leak channels; the solid yellow channels represent chloride leak channels.
‘Potassium Flow at Rest’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Commercial Share-Alike (CC BY-NC-SA) 4.0 International License. View static image of animation.
Resting Membrane Potential Value

The resting membrane potential of a neuron is typically around -65mV to -70mV (but can vary quite
a bit). You might ask, though, if the cell has these open non-gated ion channels, and ions are moving
at rest, won’t the cell eventually reach potassium’s equilibrium potential if the membrane is only
permeable to potassium?
If the open non-gated potassium channels were the only structural ion flow element present in the
cell membrane, the membrane potential would eventually reach potassium’s equilibrium potential.
However, the membrane has other open non-gated ion channels as well. Although, there are fewer of
these channels compared to the potassium channels. The permeability of chloride is about half that
of potassium and the permeability of sodium is about 25 to 40 times less than that of potassium.
This leads to enough chloride and sodium ion movement to keep the neuron at a resting membrane
potential that is slightly more positive than potassium’s equilibrium potential.
Animation 7.2. The membrane at rest is most permeable to potassium and this leads to potassium
efflux. However, the membrane is also permeable to chloride and sodium, so the flow of these ions
keeps the resting membrane potential more positive than potassium’s equilibrium potential. The
dotted, blue channels represent sodium leak channels; the striped, green channels represent potassium
leak channels; the solid yellow channels represent chloride leak channels. ‘Ion Flow at Rest’ by Casey
Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC BY-
NC-SA) 4.0 International License. View static image of animation.
2. Intracellular Anions
There are negatively charged anions trapped within the cell that contribute to the negative intracellular
charge when compared to the extracellular charge.
3. Activity of the Sodium/Potassium Pump

It is critical the ion gradients that exist across the membrane be maintained for proper neuronal
function.
As ions move across the membrane, both at rest and when the neuron is active, the concentrations
of ions inside and outside of the cell would change. This would lead to changes in the electrochemical
gradients that are driving ion movement. What, then, maintains the concentration and electrical
gradients critical for the ion flow that allows the neuron to function properly?
The sodium-potassium pump is the key. The pump uses energy in the form of ATP to move
three sodium ions out of the cell and two potassium ions in. This moves the ions against their
electrochemical gradients, which is why it requires energy. The pump functions to keep the ionic
concentrations at proper levels inside and outside the cell. The pump is removing three positively
charged ions from inside the cell and adding two positively charged ions. This leaves the inside of the
cell always more negative than the outside of the cell.
Animation 7.3. The sodium-potassium pump is embedded in the cell membrane and uses ATP to
move sodium out of the cell and potassium into the cell, maintaining the electrochemical gradients
necessary for proper neuron functioning. Three intracellular sodium ions enter the pump. ATP is
converted to ADP, which leads to a conformational change of the protein, closing the intracellular
side and opening the extracellular side. The sodium ions leave the pump while two extracellular
potassium ions enter. The attached phosphate molecule then leaves, causing the pump to again open
toward the inside of the neuron. The potassium ions leave, and the cycle begins again. ‘Sodium-
Potassium Pump’ by by Casey Henley is licensed under a Creative Commons Attribution Non-
Calculating Membrane Potential with Goldman

Equation
Importantly, membrane potential is affected by all of the ions that exist around the membrane. It is
possible to calculate the membrane potential of a cell if the concentrations and relative permeabilities
of the ions are known.
Recall from the last chapter, the Nernst equation is used to calculate one ion’s equilibrium
potential. Knowing the equilibrium potential can help you predict which way one ion will move,
and it also calculates the membrane potential value that the cell would reach if the membrane were
only permeable to one ion. However, at rest, the membrane is permeable to potassium, chloride, and
sodium. To calculate the membrane potential, the Goldman equation is needed.
The Goldman Equation
Like the Nernst equation, the constant 61 is calculated using values such as the
universal gas constant and temperature of mammalian cells
Pion is the relative permeability of each ion
[Ion]inside is the intracellular concentration of each ion
[Ion]outside is the extracellular concentration of each ion
Example: The Neuron at Rest
[table id=2 /]
Potassium Levels Must be Regulated within the

Brain
Clearly, potassium levels must be tightly regulated due to the permeability of potassium for the
neuronal membrane. Due to the importance of maintaining appropriate potassium levels, the brain
has a specialized structure called the Blood Brain Barrier that helps to maintain proper extracellular
potassium in the brain by limiting the amount of potassium that can move from capillaries into the
brain. Without the blood brain barrier, eating foods that are high in potassium, like a banana, could
completely halt the function of your brain!
Typically, capillaries are very leaky vessels, allowing a variety of different nutrients and waste
products to pass between the capillaries and the body tissues. The capillaries in the brain, however,
are surrounded by astrocytes (a type of glia that we learned about in Chapter 3). The addition of the
astrocytes makes it more difficult for substances to pass between the blood and the brain tissue.
In addition to blocking the movement of potassium from the blood to the brain tissue, astrocytes
also have potassium pumps in the astrocyte membranes that actively pump potassium out of the
extracellular fluid and into the astrocytes to help regulate extracellular potassium levels. This is called
potassium buffering.
Figure 7.3. Blood

Brain Barrier.
Capillary vessels
are typically lined
with epithelial
cells wtih leaky
junctions
between
adjacent cells
that allow for
transfer of
substances
between
circulation and
the body tissues.
Within the brain,
astrocyte
podocytes (feet)
surround the
capillaries,
forming tight
junctions that
provide another
barrier to the
movement of
substances
between
circulation and
neural tissues.
This blood brain
barrier restricts
the movement of
many different
things to help
keep the solution
surrounding the
brain cells fairly
consistent, which
is especially
important in
maintaining
potassium
concentration.
Key Takeaways
• Non-gated (leak) potassium channels are open at rest causing potassium to have the
highest permeability at rest
• Other ion channels (chloride and sodium) are also open, but fewer are open than
potassium
• The resting membrane potential of a typical neuron is relatively close to the
equilibrium potential for potassium
• The sodium-potassium pump is responsible for maintaining the electrochemical
gradients needed for neuron functioning
• The membrane potential can be calculated by knowing the concentrations of each
ion inside and outside the cell membrane and the permeabilities of each ion
Test Yourself!
Attributions
Media Attributions
• Blood Brain Barrier © Armin Kübelbeck adapted by Valerie Hedges is licensed under a CC BY
88 | ELECTRICAL ACTIVITY IN NEURONS
8.
ELECTRICAL ACTIVITY IN NEURONS
The membrane potential will always fall into one of the

following categories:
Resources
1. Resting membrane potential
2. Graded Potentials (postsynaptic potentials)
3. Action potentials
• Glossary Terms
• Key Takeaways
When the neuron is at rest, there is a baseline level of ion
• Test Yourself
flow through leak channels. However, the ability of
neurons to function properly and communicate with
other neurons through action potentials relies on ion
flow through channels other than the non-gated leak channels. We will cover how these channels open
in a later lesson. This chapter will examine ion flow through these channels after a stimulus and how
the membrane potential changes in response.
Postsynaptic Potentials (Graded Potentials)

Postsynaptic potentials (also called graded potentials) are changes in membrane potential that move
the cell away from its resting state. For our purposes, postsynaptic potentials are measured in the
dendrites and cell bodies. Ion channels that are opened by a stimulus allow brief ion flow across the
membrane.
A stimulus can range from neurotransmitters released by a presynaptic neuron, changes in the
extracellular environment like exposure to heat or cold, interactions with sensory stimuli like light or
odors, or other chemical or mechanical events. The change in membrane potential in response to the
stimulus will depend on which ion channels are opened by the stimulus.
ELECTRICAL ACTIVITY IN NEURONS | 89
Animation 8.1. A stimulus can cause ion channels in the membrane of the cell body or dendrites to
open, allowing ion flow across the membrane. The dotted, blue channels represent sodium channels;
the striped, green channels represent potassium channels; the solid yellow channels represent chloride
channels. ‘Postsynaptic Ion Flow’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License. View static
image of animation.
Depolarization: Excitatory Postsynaptic Potentials

(EPSPs)
An Excitatory Postsynaptic Potential (EPSP) occurs when sodium channels open in response to
a stimulus. The electrochemical gradient drives sodium to rush into the cell. When sodium brings its
positive charge into the cell, the cell’s membrane potential becomes more positive, or depolarizes.
This change is called a depolarization because the cell’s membrane potential is moving toward 0 mV,
and the membrane is becoming less polarized. At 0 mV, there is no potential or polarization across
the membrane, so moving toward 0 would be a decrease in potential. This depolarization increases
the likelihood a neuron will be able to fire an action potential, which makes this ion flow excitatory.
Therefore, an EPSP is an excitatory change in the membrane potential of a postsynaptic neuron.
A postsynaptic potential is typically brief, with ion channels closing quickly after the stimulus
occurs. If there is not another stimulus, the cell will return to the resting membrane potential.
Animation 8.2. When a stimulus opens sodium channels, sodium rushes into the cell because the
equilibrium potential of sodium is +60 mV. This causes an excitatory depolarization called an
excitatory postsynaptic potential (EPSP). After the stimulus, the ion channels close, and the
membrane potential returns to rest. The dotted, blue channels represent sodium channels; the striped,
green channels represent potassium channels; the solid yellow channels represent chloride channels.
‘EPSP’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-
Alike (CC BY-NC-SA) 4.0 International License. View static image of animation.
Hyperpolarization: Inhibitory Postsynaptic Potentials

(IPSPs)
An inhibitory postsynaptic potential, or IPSP, on the other hand, is caused by the opening of
chloride channels. The equilibrium potential of chloride is -65 mV, so if the neuron is at rest at -60
mV, the electrochemical gradients drive chloride to flow into the cell when chloride channels open.
Chloride brings its negative charge into the cell, causing the cell’s membrane potential to become more
negative, or hyperpolarize. This change is called a hyperpolarization because the cell’s membrane
potential is moving away from 0 mV, and the membrane is becoming more polarized. An IPSP
decreases the likelihood a neuron will be able to fire an action potential, which make this ion flow
inhibitory. Therefore, an IPSP is an inhibitory change in the membrane potential of a postsynaptic
neuron.
An IPSP can also be caused by the opening of potassium channels. The equilibrium potential of
potassium is -80 mV, so if the neuron is at rest at -60mV, the electrochemical gradients drive potassium
out of the cell when potassium channels open. As positively charged potassium ions leave the cell,
this causes the cell’s membrane potential to hyperpolarize. This demonstrates the importance of the
equilibrium potential in driving ion movement.
Like an EPSP, an IPSP is also typically brief, and the membrane potential will return to rest if no
additional stimulation occurs.
can view them online here: https://openbooks.lib.msu.edu/introneuroscience1/?p=48#video-48-3
Animation 8.3. When a stimulus opens chloride channels, and the resting membrane potential is more
positive than chloride’s equilibrium potential of -65 mV, chloride rushes into the cell. This causes an
inhibitory hyperpolarization called an inhibitory postsynaptic potential (IPSP). After the stimulus, the
ion channels close, and the membrane potential returns to rest. The dotted, blue channels represent
sodium channels; the striped, green channels represent potassium channels; the solid yellow channels
represent chloride channels. ‘IPSP’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License. View static
image of animation.
The Resting Membrane Potential is Critical

In the previous example, the resting membrane potential of that cell was -60 mV, so chloride moved
into the cell. If the resting membrane potential was instead equal to chloride’s equilibrium potential
of -65 mV, then chloride would be at equilibrium and move into and out of the cell; there would be
no net movement of the ion. Even though this would lead to no change in membrane potential, the
opening of chloride channels continues to be inhibitory. Increased chloride conductance would make
it more difficult for the cell to depolarize and to fire an action potential.
Animation 8.4. If the cell is at rest at chloride’s equilibrium potential, when a stimulus opens the
chloride channels, there will be no net movement of chloride in either direction because chloride will
be at equilibrium. Since there is no net movement, there will also be no change in membrane potential
because there is an equal amount of ion flow into and out of the cell. The dotted, blue channels
represent sodium channels; the striped, green channels represent potassium channels; the solid yellow
channels represent chloride channels. ‘IPSP at Equilibrium’ by Casey Henley is licensed under a
License. View static image of animation.
If the resting membrane potential of the cell was more negative than chloride’s equilibrium
potential—for example, at -70 mV—then chloride would leave the cell in order to move the membrane
potential toward -65 mV. This would result in a depolarization of the membrane potential. However,
the overall effect is still inhibitory because once the cell reaches -65 mV, the driving forces acting on
chloride would try to keep the cell at that membrane potential, making it more difficult for the cell to
depolarize further and fire an action potential.
A good rule of thumb is to remember that opening of sodium channels is excitatory whereas
opening of potassium or chloride channels is typically inhibitory.
Animation 8.5. If the cell is at rest at chloride’s equilibrium potential, chloride will leave the cell when
a stimulus opens the chloride channels, removing its negative charge. This causes a depolarization in
the membrane potential, but it is still inhibitory since chloride movement will try to keep the cell near
-65 mV. The dotted, blue channels represent sodium channels; the striped, green channels represent
potassium channels; the solid yellow channels represent chloride channels.‘Inhibitory Depolarization’
(CC BY-NC-SA) 4.0 International License. View static image of animation.
Summation of Inputs
If an excitatory stimulus is followed by additional excitatory stimuli, the sodium channels will either
remain open or additional sodium channels will open. The increased sodium conductance will cause
the EPSPs to summate, depolarizing the cell further than one EPSP alone. Each neuron has a
threshold membrane potential at which the cell will fire an action potential. The summation of EPSPs
causes the neuron to reach that threshold.
Animation 8.6. Excitatory stimuli that occur quickly in succession lead to summation of EPSPs. This
leads to increased depolarization of the membrane potential compared to a single EPSP. The dotted,
blue channels represent sodium channels; the striped, green channels represent potassium channels;
the solid yellow channels represent chloride channels. ‘Summated EPSP Ion Flow’ by Casey Henley is
licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0
International License. View static image of animation.
Summation can occur in two ways. Temporal summation occurs when one presynaptic input
stimulates a postsynaptic neuron multiple times in a row. Spatial summation occurs when multiple
presynaptic inputs each stimulate the postsynaptic neuron at the same time. Both types of summation
result in a depolarization of a higher magnitude than when only a single excitatory input occurs.
Figure 8.1 EPSPs can summate via temporal or spatial summation. Temporal summation occurs
when a presynaptic neuron, Input 1 in the figure, stimulates the postsynaptic neuron multiple times
in a row. Spatial summation occurs when more than one presynaptic neuron (inputs 1 through 4 in
the figure) each stimulate the postsynaptic neuron at the same time. The EPSPs of each stimulation
will add together to cause a stronger depolarization of the membrane potential of the postsynaptic
neuron than one excitatory stimulus alone. ‘Synaptic Summation” by Casey Henley is licensed under
a Creative Commons Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International
License.
In addition to the summation of excitatory inputs, EPSPs can also summate with inhibitory inputs.
The addition of an inhibitory stimulus will result in either a weaker depolarization compared to a single
excitatory stimulus, or possibly no depolarization at all depending on the strength of the inhibitory
input.
Figure 8.2. If an inhibitory input (input 3 in the figure) stimulates the postsynaptic neuron at the
same time as an excitatory input (input 1 in the figure), the result is a decrease in the amount of
depolarization or the complete prevention of depolarization depending on the strength of the
inhibitory input. ‘EPSP and IPSP Summation’ by Casey Henley is licensed under a Creative Commons
In the case of combined inhibitory and excitatory stimuli, both chloride and sodium channels will
open. As sodium enters the cell, trying to move the membrane potential to +60 mV (the equilibrium
potential of sodium) chloride will also enter, trying to keep the cell near -65 mV (the equilibrium
potential of chloride).
Animation 8.7. When an inhibitory input and an excitatory input stimulate a postsynaptic neuron at
the same time, chloride and sodium channels open. Due to the equilibrium potentials of the two ions,
both will flow into the cell. Sodium tries to depolarize the cell, whereas chloride tries to keep the cell
near rest. The dotted, blue channels represent sodium channels; the striped, green channels represent
potassium channels; the solid yellow channels represent chloride channels. ‘EPSP and IPSP Ion Flow’
Key Takeaways
• Postsynaptic potentials occur in the dendrites or cell body

• Excitatory postsynaptic potentials are caused by sodium channels opening
• Inhibitory postsynaptic potentials are caused by chloride channels opening
• Since the resting membrane of a typical neuron is usually very close to chloride’s
equilibrium potential, knowing and comparing these two values is important for
determining direction of ion flow when chloride channels open
• Input effects, whether excitatory or inhibitory, can summate and affect the
postsynaptic neuron’s membrane potential
Test Yourself!
Attributions
98 | ACTION POTENTIALS
9.
ACTION POTENTIALS
As covered in previous chapters, the action potential is a

very brief change in the electrical potential, which is the
Resources difference in charge between the inside and outside of the
cell. During the action potential, the electrical potential
across the membrane moves from a negative resting value
to a positive value and back.
• Glossary Terms
• Key Takeaways
• Test Yourself
ACTION POTENTIALS | 99
Figure 9.1. The action potential is a brief but significant change in electrical potential across the
membrane. The membrane potential will begin at a negative resting membrane potential, will
rapidly become positive, and then rapidly return to rest during an action potential. ‘Action Potential’
Propagation
The propagation of the action potential from the axon hillock down the axon and to the
presynaptic terminal results in the release of chemical neurotransmitters that communicate with a
postsynaptic neuron.
Animation 9.1. The action potential moves down the axon beginning at the axon hillock. The action
potential moving down a myelinated axon will jump from one Node of Ranvier to the next. This
saltatory conduction leads to faster propagation speeds than when no myelin in present. When the
action potential reaches the synaptic terminal, it causes the release of chemical neurotransmitter.
‘Action Potential Propagation’ by Casey Henley is licensed under a Creative Commons Attribution
Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License. View static image of
animation.
Voltage-Gated Ion Channels

The change in membrane potential during the action potential is a function of ion channels in the
membrane. In the previous lessons, we have learned about the principles of ion movement and have
discussed non-gated (leak) channels at rest, as well as ion channels involved in the generation of
postsynaptic potentials. In this chapter, we will examine a different type of ion channel: voltage-
gated ion channels. For our purposes, these channels are located primarily at the axon hillock, along
the axon, and at the terminal. They are necessary for the propagation of the action potential.
Figure 9.2. Voltage-gated channels critical for the propagation of the action potential are located at
the axon hillock, down the axon at the Nodes of Ranvier, and in the presynaptic terminal.
‘Voltage-Gated Channel Location’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License.
Voltage-gated channels allow ions to cross the membrane using the same ion movement principles
covered in previous lessons. The main difference between voltage-gated channels and leak channels
are how they are opened or “gated”. Voltage-gated channels open when the cell’s membrane potential
reaches a specific value, called threshold. The neuron reaches threshold after enough EPSPs summate
together.
Animation 9.2. As EPSPs summate, a result of ion movement not shown in the animation, the cell’s
membrane potential will depolarize. Reaching threshold causes voltage-gated ion channels to open.
Once the channels are open, ions will move toward equilibrium. In the animation, sodium ions
flow inward. The dotted, blue channels represent voltage-gated sodium channels; the striped, green
channels represent voltage-gated potassium channels; the solid yellow channels represent chloride
channels. ‘Voltage-Gated Channel’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License. View static
image of animation.
The Action Potential

The action potential begins when the cell’s membrane potential reaches threshold. Once initiated in a
healthy, unmanipulated neuron, the action potential has a consistent structure and is an all-or-nothing
event. It will run through all the phases to completion.
The rising phase is a rapid depolarization followed by the overshoot, when the membrane
potential becomes positive. The falling phase is a rapid repolarization followed by the undershoot,
when the membrane potential hyperpolarizes past rest. Finally, the membrane potential will return
to the resting membrane potential.
Figure 9.3. EPSPs that summate to reach threshold initiate the action potential. The depolarizing
rising phase moves the membrane potential from threshold to above 0 mV. The overshoot is the
peak of the action potential where the membrane potential is positive. The falling phase repolarizes
the membrane potential, and the undershoot takes the membrane potential more negative than the
resting membrane potential. After the undershoot, the membrane potential returns to rest. ‘Action
Potential Phases’ by Casey Henley is licensed under a Creative Commons Attribution
Rising Phase
The rising phase is caused by the opening of voltage-gated sodium channels. These ion channels are
activated once the cell’s membrane potential reaches threshold, opening immediately. The
electrochemical gradients drive sodium into the cell causing the depolarization.
Animation 9.3. Voltage-gated sodium channels open once the cell’s membrane potential reaches
threshold. The rapid influx of sodium results in a large depolarization called the rising phase. The
dotted, blue channels represent voltage-gated sodium channels; the striped, green channels represent
voltage-gated potassium channels; the solid yellow channels represent chloride channels. ‘Rising Phase’
(CC-BY-NC-SA) 4.0 International License. View static image of animation.
Falling Phase
The falling phase of the action potential is caused by the inactivation of the sodium channels and the
opening of the potassium channels. After approximately 1 msec, the sodium channels inactivate. The
channel becomes blocked, preventing ion flow. At the same time, the voltage-gated potassium channels
open. This allows potassium to rush out of the cell because of the electrochemical gradients, taking
its positive charge out of the cell and repolarizing the membrane potential, which returns the cell’s
membrane potential back near rest.
Like the voltage-gated sodium channels, the voltage trigger for the potassium channel is when
the cell’s membrane potential reaches threshold. The difference is that the sodium channels open
immediately, whereas the potassium channels open after a delay.
Animation 9.4. After approximately 1 msec, the voltage-gated sodium channels inactivate, which
prevents any further ion flow into the cell. Although the voltage-gated potassium channels are activated
in response to the cell reaching threshold, their opening is delayed and occurs alone with the sodium
channel inactivation. This allows an efflux of potassium ions, which causes the repolarization of the
falling phase. The dotted, blue channels represent voltage-gated sodium channels; the striped, green
channels. ‘Falling Phase” by Casey Henley is licensed under a Creative Commons Attribution Non-
Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License. View static image of animation.
Undershoot
As the membrane potential returns to resting level, the sodium channels will de-inactivate, returning
to the closed position, ready to be opened again by another voltage change. The potassium channels
will also close, but they remain open long enough to cause a hyperpolarizing undershoot as potassium
continues to move toward its equilibrium potential of -80 mV.
Animation 9.5. Once the cell’s membrane potential repolarizes, the voltage-gated sodium channels de-
inactivate and return to their closed state. The voltage-gated potassium channels remain open long
enough for the undershoot to occur as potassium continues to flow out of the cell. The dotted, blue
channels represent voltage-gated sodium channels; the striped, green channels represent voltage-gated
potassium channels; the solid yellow channels represent chloride channels. ‘Undershoot’ by Casey
Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC-BY-
Return to Rest
Once the voltage-gated channels close, the sodium-potassium pumps will reestablish the proper ionic
concentrations needed for the electrochemical gradients. This action, along with open leak channels,
will return the cell to its resting membrane potential.
Animation 9.6. Once the voltage-gated potassium channels close, the sodium-potassium pump will
work to re-establish the electrochemical gradients and return the cell to its resting membrane potential.
‘Return to Rest’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Refractory Periods
The Absolute Refractory Period

Each neuron does have a maximum firing rate. And even if the stimulus continues to increase in
strength, the neuron cannot fire at a higher frequency. The maximum firing rate of a cell is determined
by the status of the ion channels in the neuronal membrane during the different phases of the action
potential. During the absolute refractory period, a second action potential cannot be fired under
any circumstances, regardless of the strength of the stimulus. The voltage-gated sodium channels are
either open (during the rising phase) or inactivated (during the falling phase).
The Relative Refractory Period

When the cell repolarizes and the voltage-gated sodium channels de-inactivate and return to a closed
state, the cell is again able to fire another action potential. However, during the end of the falling
phase and the during the undershoot, voltage-gated potassium channels are still open. During the
undershoot, while the neuron is hyperpolarized, a larger-than-normal stimulus is needed to make
the cell reach threshold again. This segment of the action potential is called the relative refractory
period. Action potentials can be fired, but a stronger stimulus is needed than when the cell is at rest.
Figure 9.4. The maximum firing rate of a neuron is determined by the refractory periods. A) During
the absolute refractory period, no additional action potentials can be fired because the
voltage-gated sodium channels are either already open (rising phase) or inactivated (falling phase).
In these states, they cannot be opened again to begin a second action potential. B) The relative
refractory period occurs when the voltage-gated sodium channels are closed, but the open
voltage-gated potassium channels cause a hyperpolarization of the membrane. After the potassium
channels close, it takes a short period of time for the membrane potential to return to rest. Action
potentials can be fired during this time, but a stronger stimulus is required to reach threshold
compared to when the cell is at rest. The dotted, blue channels represent voltage-gated sodium
channels; the striped, green channels represent voltage-gated potassium channels; the solid yellow
channels represent chloride channels. ‘Refractory Periods” by Casey Henley is licensed under a
Creative Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International
License.
Action Potential Characteristics

For a given cell, all action potentials have the same characteristics; they depolarize to the same
membrane potential value and take the same amount of time. However, different neurons may exhibit
different action potential characteristics. Likewise, if a neuron has a change in its environment, like
altered extracellular ion concentrations, the shape of the action potential would change due to a change
in the electrochemical gradients. For example, if the external concentration of sodium is decreased, the
equilibrium potential of sodium (as well as the strength of the electrochemical gradients) will change,
which will result in a slower rate of rise and a lower amplitude of the action potential.
Figure 9.5. A) A neuron kept under the same conditions will display action potentials of similar
height and length. B) However, if cellular conditions change, so will the action potential
characteristics. If extracellular sodium levels are decreased compared to control levels, the action
potential will show a slower rate of rise and a decreased height. ‘Low Sodium Action Potential’ by
Stimulus Strength
The strength of a stimulus needs to be encoded by the neurons. We need to be able to perceive
the difference, for example, between a dim light and a bright one. The frequency, or rate, of action
potential firing informs the nervous system of stimulus strength.
Since the height of the action potential is always the same for a given neuron, the strength of the
stimulus is determined by the frequency of action potential firing. A weak stimulus would cause fewer
action potentials to be fired than a strong stimulus.
Figure 9.6. Information about the strength of a stimulus is encoded by the rate of action potential
firing. A) A weak stimulus results in few action potentials being fired. B) A strong stimulus results in
many action potentials firing in a row. ‘Stimulus Strength’ by Casey Henley is licensed under a
License.
Direction of Propagation
The action potential moves down the axon due to the influx of sodium depolarizing nearby axon
segments to threshold.
Animation 9.7. A voltage change that reaches threshold will cause voltage-gated sodium channels to
open in the axonal membrane. The influx of sodium causes the rising phase of the action potential, but
the ion flow also depolarizes nearby axon regions. As the depolarization reaches threshold, the action
potential moves down the axon. The dotted, blue channels represent voltage-gated sodium channels;
the striped, green channels represent voltage-gated potassium channels. ‘Action Potential Movement’
(CC-BY-NC-SA) 4.0 International License. View static image of animation.
Action potentials only move in one direction, from the cell body to the presynaptic terminal. The
refractory period keeps the action potential from moving backward down the axon. As the action
potential moves from one Node of Ranvier to the next, the inactivated sodium channels in the
previous axon segment prevent the membrane from depolarizing again. Therefore, the action potential
can only move forward toward axon segments with closed sodium channels ready for rising phase
depolarization.
Figure 9.7. Action potentials only travel in one direction. The inactivated sodium channels prevent
the action potential from moving backward down the axon. Blue dotted channels: sodium channels;
green striped channels: potassium channels. The dotted, blue channels represent voltage-gated
sodium channels; the striped, green channels represent voltage-gated potassium channels. ’No
Backward Propagation’ by Casey Henley is licensed under a Creative Commons Attribution
Speed of Propagation
Presence of Myelin
The presence of myelin leads to a significant increase in action potential conduction speed compared
to an unmyelinated axon. For a myelinated axon, the action potential “jumps” between Nodes of
Ranvier in a process called saltatory conduction. The nodes have a high density of voltage-gated
channels, and the action potential is able to skip the axon segments covered by the myelin. In an
unmyelinated axon, the action potential moves in a continuous wave. In additional to the saltatory
conduction process, the presence of myelin also insulates the axon, preventing charge loss across the
membrane, which also increases speed of the action potential.
One or more interactive elements has been excluded from this version of the text. You can view
them online here: https://openbooks.lib.msu.edu/introneuroscience1/?p=57#video-57-8
Animation 9.8. The action potential moves down an unmyelinated axon like a wave, opening voltage-
gated channels along the length of the axon. In a myelinated axon, the action potential is able to skip
portions of the axon that are covered by the myelin; the action potential jumps from node to node
and travels further down the axon in the same amount of time. The dotted, blue channels represent
voltage-gated sodium channels; the striped, green channels represent voltage-gated potassium channels.
‘Action Potential Speed’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Diameter of Axon
The diameter of the axon also affects speed. The larger the diameter of the axon, the faster the
propagation of the action potential down the axon. A larger axon leads to less resistance against the
flow of ions, so the sodium ions are able to move more quickly to cause the regeneration of the action
potential in the next axon segment.
Figure 9.8. The diameter of the axon and the amount of myelination varies. Large diameter axons
typically have thicker myelin sheath, which results in fast action potential speed. Small diameter
axons may have no myelin present, resulting in slow action potential speed. ‘Axon Diameter’ by
Key Takeaways
• The voltage-gated ion channels are located along the axon hillock and axon; they
open in response to the membrane potential reaching a threshold value
• The rising phase of the action potential is a result of sodium influx
• The falling phase of the action potential is a result of potassium efflux
• Action potentials are all-or-none (postsynaptic potentials are graded)
• Action potential have the same height of depolarization for a given cell under typical
conditions
• The neuron cannot fire a second action potential during the absolute refractory
phase
• The neuron can fire a second action potential during the relative refractory phase,
but it requires a stronger stimulus than when the neuron is at rest
• Stimulus strength is coded by frequency of action potential firing
• Action potential travel in one direction due to the presence of inactivated voltage-
gated sodium channels
• Speed of propagation relies on presence and thickness of myelin and diameter of
axon
Test Yourself!
Attributions
116 | VOLTAGE CLAMP
10.
VOLTAGE CLAMP
In the previous chapter, we covered ion flow and

membrane potential changes that occur in the neuron
Resources during the action potential. We have this level of
understanding about how ions move during the action
potential because of a special technique called a voltage
clamp experiment that was used in the 1950s. The
• Glossary Terms
voltage clamp method allows researchers to study
• Key Takeaways
voltage-gated ion channels by controlling the
• Test Yourself
membrane potential of a neuron.
The Voltage Clamp

Experiment
Initial Set-Up
To conduct a voltage clamp experiment, a portion of the axon, which would include the cell
membrane and all the voltage-gated ion channels located there, is removed from a neuron and placed
into a solution that mimics that of physiological extracellular solution. The ion concentrations across
the membrane, as well as the electrochemical gradients, would remain the same.
VOLTAGE CLAMP | 117
Figure 10.1. To conduct a voltage clamp experiment, a portion of the axon is removed from the
neuron. The axon is placed in a special solution that is similar to physiological extracellular solution.
‘In Vitro Axon’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Measuring the Membrane Potential

The initial step in the voltage clamp method is to measure the membrane potential of the axon. A
recording electrode is placed into the axon and a reference electrode (or ground electrode) is placed
into the extracellular solution. The voltage difference between these two electrodes is the membrane
potential of the axon.
118 | VOLTAGE CLAMP
Figure 10.2. Measuring the membrane potential of the axon segment is the first step in the
voltage-clamp experiment. The membrane potential is the difference in voltage between the
intracellular recording electrode and the extracellular reference electrode. ‘Measure Membrane
Potential’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Clamping the Voltage

The researchers running the experiment can set a desired membrane potential for the cell. The
equipment then compares the desired membrane potential with the measured membrane potential
from the electrodes. If these values differ, current is injected into the cell to change the measured
membrane potential and make it equal to the desired potential.
VOLTAGE CLAMP | 119
Figure 10.3. A desired membrane potential is set for the experiment. The voltage-clamp
experimental equipment then compares the measured membrane potential with the desired
potential. Current is then injected into the axon through a current-passing electrode to make the
measured membrane potential equal to the desired potential. ‘Clamping Voltage’ by Casey Henley is
Repeat
The equipment continues this cycle for the length of the experiment. It constantly measures and
compares the actual membrane potential with the desired potential, and then uses current to correct
any changes, “clamping” the potential at one value.
120 | VOLTAGE CLAMP
Figure 10.4. The voltage clamp cycle repeats continuously. The actual membrane potential of the
axon is measured, compared to the set desired potential value, and then current is passed into the
axon to keep the actual membrane potential equal to the desired potential. ‘Voltage Clamp Cycle’ by
Voltage Clamp Experiment Example

VOLTAGE CLAMP | 121
At Rest
Let’s work through the system with an example. Here is an axon bathed in the extracellular
solution. The resting membrane potential is measured at -65 mV.
Figure 10.5. Measure the membrane potential. The membrane potential of this axon at rest is -65 mV.
‘Voltage Clamp Example at Rest’ by Casey Henley is licensed under a Creative Commons Attribution
Set Clamped Membrane Potential Value
For this experiment, the desired membrane potential value is 0 mV.

122 | VOLTAGE CLAMP
Figure 10.6. Set desired membrane potential. The set value for this experiment is 0 mV. ‘Voltage
Clamp Example Set Value’ by Casey Henley is licensed under a Creative Commons Attribution
Compare Actual and Set Membrane Potential Values
The equipment will determine that the actual membrane potential of the cell is not correct
(-65 mV compared to 0 mV), so the cell must depolarize to reach the set value.
VOLTAGE CLAMP | 123
Figure 10.7. Compare measured membrane potential to desired potential. The actual membrane
potential of the axon is at -65 mV, so the cell needs to be depolarized to reach the desired potential of
0 mV. ‘Voltage Clamp Example Comparison’ by Casey Henley is licensed under a Creative Commons
Adjust Membrane Potential
To make the axon move from its resting membrane potential to 0 mV, the current electrode
will pass positive current into the cell, depolarizing the cell until the membrane potential
reaches the set value.
124 | VOLTAGE CLAMP
Figure 10.8. Correct actual membrane potential. To depolarize this axon from rest at -65 mV to the
desired clamp value of 0 mV, positive current will be injected into the cell. The membrane potential
will then depolarize to 0 mV and remain there. ‘Voltage Clamp Example Current’ by Casey Henley is
Ion Channels Continue to Function During

Voltage Clamp
The important aspect of the depolarization seen in the example is that it is above threshold. Moving
the membrane potential above threshold will activate the voltage-gated ion channels. Sodium
channels will open immediately, and sodium will begin rushing into the cell. This influx of positive
ions would normally cause the membrane potential to depolarize, but the voltage clamp equipment
will measure the ion flow and inject a current of equal strength and opposite charge into the axon to
maintain the membrane potential at 0 mV. This happens almost instantly and is a constant process,
so, as the ion flow changes, so does the injected current.
VOLTAGE CLAMP | 125
Animation 10.1. Clamping the cell at 0 mV will result in current being passed into the axon to
depolarize the membrane potential. This depolarization is above threshold, so the voltage-gated ion
channels in the membrane will be activated. Sodium will enter the axon through the open sodium
channels. The voltage clamp equipment will inject current equal in strength and opposite in charge
to the sodium influx in order to keep the membrane potential of the axon at 0 mV. The membrane
potential will remain at 0 mV because the injected current offsets any change that would normally
occur due to ion flow. ‘Voltage Clamp Sodium Flow’ by Casey Henley is licensed under a Creative
View static image of animation.
Since the ion channels function as expected during the voltage clamp experiment, the voltage-gated
sodium channels will inactivate and the delayed voltage-gated potassium channels will open. This is
because, like the sodium channels, they are also activated when the membrane potential reaches
threshold. This causes the ion flow to change from inward to outward. Normally, potassium efflux
would cause a repolarization of the membrane potential, but the voltage clamp equipment will again
inject a current that is equal in strength and opposite in charge to the potassium flow to keep the
membrane potential steady at 0 mV.
Animation 10.2. The voltage-gated sodium channels will inactivate and the potassium channels will
open. Potassium will then flow out of the axon. Similar to the sodium influx, the voltage clamp
equipment will inject current equal in strength and opposite in charge to the potassium efflux in
126 | VOLTAGE CLAMP
order to keep the membrane potential of the axon at 0 mV. ‘Voltage Clamp Potassium Flow’ by Casey
Data Collection
Researchers can determine how much current is moving through the voltage-gated ion channels by
observing how much current the equipment must inject into the cell to keep the membrane potential
steady. If the equipment has to inject negative current in for 2 milliseconds, then the researchers know
that positive ions were flowing in for 2 milliseconds. The voltage-clamp setup allowed researchers in
the 1950s to learn about how the voltage-gated ion channels were functioning during an action
potential.
Key Takeaways
• The membrane potential does not change during a voltage clamp experiment
• Voltage-gated ion channels are still able to function normally and allow ion flow
• If the clamped membrane potential is above threshold, the voltage-gated channels
will act as if the cell is firing an action potential
• The equipment must compensate for the neuron’s ion flow by injecting current into
the axon. The amount of current needed to keep the membrane potential steady is
equal and opposite to the current actually flowing in the cell
VOLTAGE CLAMP | 127
Test Yourself!
Attributions
128 | VOLTAGE CLAMP
NEURONAL COMMUNICATION | 129
PART III
NEURONAL COMMUNICATION
130 | NEURONAL COMMUNICATION
SYNAPSE STRUCTURE | 131
11.
SYNAPSE STRUCTURE
For the nervous system to function, neurons must be able

to communicate with each other and they do this through
structures called synapses. At the synapse, the terminal of Resources
a presynaptic cell comes into close contact with the cell
membrane of a postsynaptic neuron.
• Glossary Terms
• Key Takeaways
• Test Yourself
132 | SYNAPSE STRUCTURE
Figure 11.1. The terminal of a presynaptic neuron comes into close contact with a postsynaptic cell at
the synapse. ‘Synapse’ by Casey Henley is licensed under a Creative Commons Attribution
Synapse Types
There are two types of synapses: electrical and chemical.
Electrical
Electrical synapses are a direct connection between two neurons. Imagine two neurons that are
connected by an electrical synapse. First of all, both of them are complete cells on their own. Each
one contains a complete plasma membrane surrounding the neuron, a nucleus, and all the individual
organelles needed to carry out that cell’s basic life processes.
Cell membrane proteins called connexons form gap junctions between the neurons. The gap
junctions form pores that allow ions to flow between neurons, so, as an action potential propagates
in the presynaptic neuron, the influx of sodium can move directly into the postsynaptic neuron and
depolarize the cell. The response in the postsynaptic cell is almost immediate, with little-to-no delay
between signaling in the pre- and postsynaptic neurons.
Animation 11.1. Membrane-bound proteins called connexons form gap junctions between
presynaptic and postsynaptic neurons. This allows for direct exchange of ions between neurons. An
action potential in the presynaptic neuron will cause an immediate depolarization of the postsynaptic
membrane because the sodium ions will cross the membrane through the gap junctions. ‘Electrical
Synapse – Ion Flow’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Since the gap junctions allow diffusion of ions without any obstruction, the signal can flow
bidirectionally through an electrical synapse. The electrochemical gradients will drive direction of
ion flow.
This means that a signal does not always move sequentially from the presynaptic cell to the
postsynaptic cell. Rather, ions and signaling molecules are free to move through the connexons in
either direction. Also, each cell within an electrically-coupled network can receive inputs at any of the
cells, making it able to detect several signals at once—the same way a huge satellite dish can detect more
signals than a small dish.
Electrical synapses likely evolved because of evolutionary pressures that selected for speed. These
synapses can pass signals as fast as electrical charges can move through an electrolyte-rich fluid like
cytoplasm; almost instantaneous.
Another advantage of electrical synapses is that they can form a large network of interconnected
neurons with synchronized activity. For example, neuroendocrine cells in the hypothalamus are
connected by electrical synapses. When the “go” signal arrives, all the cells depolarize at once, which
can result in the massive release of hormones into the bloodstream. A network can also cause sudden,
powerful inhibition. Like an angry mob of people chanting, a network of electrical synapses
connecting inhibitory interneurons allows the network to send an immediate “shutdown” signal under
specific circumstances.
Animation 11.2. Since an electrical synapse is a direct, physical connection between two neurons, ions
are able to flow either direction across the gap junction. ‘Bidirectional Electrical Synapse’ by Casey
Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC BY-
Electrical synapses share the cytoplasm between the two connected cells, so ions, ATP, and larger
signaling molecules and proteins are able to move between the two cells. These signaling molecules play
an important role in cellular mechanisms, which we will see in a later chapter.
Animation 11.3. Gap junctions are large enough to allow the flow of small cellular molecules like
ATP or second messengers. ‘Electrical Synapse – Small Molecules’ by Casey Henley is licensed under
Chemical
At a chemical synapse, a signaling molecule is released by the presynaptic cell to influence the
postsynaptic cell by binding to postsynaptic receptors. Since chemical synapses do not rely on a direct
physical protein “tunnel” to connect the two neurons, the distance between the two cells can be much
larger.
On average, a chemical synapse is a distance of about 20-40 nanometers, roughly a thousand times
smaller than the diameter of a human hair. A chemical synapse can pass a variety of signals, depending
on the neurotransmitter and the receptor. For example, some signals are directly excitatory and allow
positively charged cations to enter the neuron causing depolarization. Other signals are
hyperpolarizing, and therefore inhibitory. And yet other signals are much more complex, inducing
changes in protein expression that can modify cellular excitability over the course of minutes or hours.
Figure 11.2. A chemical synapse does not make direct contact between the two neurons. The
presynaptic terminal and the postsynaptic membrane are separated by the synaptic cleft.
Neurotransmitters are stored in the presynaptic cell, and the postsynaptic cell has neurotransmitter
receptors in the membrane. ‘Chemical Synapse’ by Casey Henley is licensed under a Creative
Commons Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License.
At a chemical synapse, the depolarization of an action potential reaching the presynaptic terminal
causes release of neurotransmitters. These neurotransmitters are synthesized and stored in neurons.
After being released, these neurotransmitters diffuse randomly across the synapse, where they are able
to affect nearby neurons once the chemical binds to its corresponding receptor located in the cell
membrane of the postsynaptic neuron. The structure and function of chemical synapses make them
slower than electrical synapses and permit signaling in only one direction.
Because of the complexity of the signals that chemical synapses can convey, evolutionary
development through time has allowed for a tremendous variety of responses. Chemical synapses allow
for fine-tuning of neural networks, giving these nervous systems a larger range of possibilities. The
nervous systems of “higher” organisms like humans tend to have several chemical synapses since these
signals are likely necessary for complex behaviors and cognition.
Animation 11.4. An action potential causes release of neurotransmitters from the presynaptic terminal
into the synaptic cleft. The transmitters then act on neurotransmitter receptors in the postsynaptic
membrane. ‘Chemical Synapse – Neurotransmitter Release’ by Casey Henley is licensed under a
Synapse Location
As we discuss synaptic transmission, we will focus mainly on axodendritic synapses, in which the
presynaptic terminal (axon) synapses on the dendrites of the postsynaptic cell. But synapses can also
be located between the terminal and the cell body of the postsynaptic cell, called axosomatic, or even
between the terminal and the axon of the postsynaptic cell, called axoaxonic.
Figure 11.3. A) Axodendritic synapses occur when the presynaptic terminal makes a synaptic
connection with the dendrite of a postsynaptic neuron. B) Axosomatic synapses occur when the
presynaptic terminal makes a synaptic connection with the cell body of a postsynaptic neuron. C)
Axoaxonic synapses occur when the presynaptic terminal makes a synaptic connection with the
axon of a postsynaptic neuron. ‘Chemical Synapse Types’ by Casey Henley is licensed under a
License.
Key Takeaways
• Electrical synapses make direct contact between neurons, are faster than chemical
synapses, and can be bidirectional
• Chemical synapses form a synaptic cleft between the neurons and are unidirectional
• Synapses can occur between the presynaptic terminal and the postsynaptic dendrites
(axodendritic), cell body (axosomatic), or axon (axoaxonic)
Test Yourself!
Attributions
STEPS IN SYNAPTIC SIGNALING | 139
12.
STEPS IN SYNAPTIC SIGNALING
Steps in Chemical
Signaling Resources
As we have covered, when an action potential propagates

down the axon to the presynaptic terminal, the electrical
signal will result in a release of chemical neurotransmitters • Glossary Terms
• Key Takeaways
that will communicate with the postsynaptic cell.
• Test Yourself
At a chemical synapse, the process of neurotransmitter
release is very tightly regulated. If there were no
mechanisms to control the release of chemicals at the
synapse, nerve cells would deplete their entire stock of
neurotransmitter. Regulation of release depends on several proteins that are important parts of the
process. These proteins are often embedded within cell membranes of the vesicles or the neuronal
membrane.
Step 1: An action potential arrives at the axon

terminal
There are a series of steps that take place during chemical synaptic transmission.
First, an action potential propagates down the axon until it arrives at the axon terminal, depolarizing
the membrane of the presynaptic terminal.
140 | STEPS IN SYNAPTIC SIGNALING
Animation 12.1. The action potential is a brief, but significant, change in electrical potential across the
membrane. The membrane potential will move from a negative, resting membrane potential (shown
here as -65 mV) and will rapidly become positive and then rapidly return to rest during an action
the synaptic terminal, it causes the release of chemical neurotransmitter. ‘Action Potential Propagation’
(CC-BY-NC-SA) 4.0 International License. View static image of animation
Step 2: Membrane depolarization from action

potential causes influx of calcium ions
When the action potential reaches the terminal, there is an influx of sodium ions. This inward positive
current causes a depolarization of the terminal, activating voltage-gated calcium channels that are
embedded in the cell membrane of the axon terminals. Due to the electrochemical gradient of calcium,
when the voltage-gated calcium channels are opened, calcium will rush into the cell. The concentration
of intracellular calcium, generally in the range of 100 nM, is much lower than the concentration
outside the cell, therefore there is a strong electrochemical gradient that moves calcium into the
2+
terminal. As it turns out, an elevation of Ca in the intracellular space is the “go ahead” signal that
causes neurotransmitter release.
Animation 12.2. An action potential causes an influx of sodium in the terminal. The depolarization
opens voltage-gated calcium channels, and calcium ions flow into the terminal down their
electrochemical gradient. The blue, dotted channels represent voltage-gated sodium channels, and the
purple, striped channels represent voltage-gated calcium channels. ‘Terminal Calcium Influx’ by Casey
Active Zones
The voltage-gated calcium channels are concentrated in the presynaptic terminal at active zones, the
regions of the membrane where small molecule neurotransmitters are released. At active zones, some
synaptic vesicles are docked and are ready for immediate release upon arrival of the action potential.
Other neurotransmitter-filled vesicles remain in a reserve pool outside of the active zone.
Vesicles filled with neuropeptides do not dock at active zones. They are located outside of the active
zone, further away from the membrane and the high density of voltage-gated calcium channels. They
are, therefore, slower to release than the small molecule transmitters.
Figure 12.1. Some synaptic vesicles filled with small molecule neurotransmitters dock at active zones
on the presynaptic membrane, ready for immediate release. Other synaptic vesicles remain nearby
in reserve pools, ready to move into empty active zones. Neuropeptide-filled vesicles do not dock at
active zones. The blue, dotted channels represent voltage-gated sodium channels, and the purple,
striped channels represent voltage-gated calcium channels. ‘Active Zones’ by Casey Henley is
Location of vesicles
Synaptic vesicles can be found in one of three places at the axon terminal.
1. Readily releasable pool. These vesicles are located close to the cell membrane at the axon
terminal. In fact, many of them are already “docked”, meaning that their coat proteins are
already interacting closely with the proteins on the inside of the cell membrane. When the
depolarizing charge of an action potential reaches the terminal, these vesicles at the readily
releasable pool are the first ones that fuse with the cell membrane and release their contents.
2. Recycling pool. These vesicles are the ones that have been depleted due to release. They are
currently in the process of being refilled or reloaded with neurotransmitter. They are farther
from the cell membrane, and the protein machinery is not primed for release, so it requires a
more intense stimulation to release the contents of these vesicles.
3. Reserve pool. These vesicles are the farthest from the surface of the cell membrane, and most
vesicles are held in this reserve pool. For these neurotransmitters to be released, very intense
stimulation is required.
Step 3: Docking of synaptic vesicles at the

membrane
Docking of synaptic vesicles packaged with small molecule neurotransmitters occurs through the
interaction of three membrane-bound proteins called SNARE proteins. Synaptobrevin is called a
v-SNARE because it is located on the Vesicular membrane. Syntaxin and SNAP-25 are called t-
SNARES because they are located on the terminal membrane, which is the Target membrane. The
interaction of these three proteins leads to vesicle docking at the active zone.
Figure 12.2. Synaptic vesicles filled with small molecule neurotransmitters are able to dock at active
zones by the interaction of v- and t-SNARE proteins. Synaptobrevin is embedded in the membrane
of the vesicle whereas SNAP-25 and Syntaxin are embedded in the presynaptic terminal membrane.
The purple, striped channels represent voltage-gated calcium channels. ‘SNARE proteins’ by Casey
Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike
The influx of calcium through the voltage-gated calcium channels initiates the exocytosis process that
leads to neurotransmitter release. Calcium enters the cell and interacts with a vesicle-bound protein
called synaptotagmin. Synaptotagmin is a calcium sensor that detects elevated levels of calcium in the
axon terminal.
In the presences of calcium, the v-SNAREs and the t-SNAREs interact with one another, forming a
molecular structure called a SNARE complex. The SNARE complex looks a lot like two twist ties that
are wound tightly together. As they twist tighter together, it causes the vesicle membrane to approach
the inside of the cell membrane, which results in vesicular fusion.
Animation 12.3. Calcium enters the cell when the voltage-gated channels open. In the presence of
calcium, synaptotagmin, a protein bound to the vesicular membrane interacts with the SNARE
proteins. The purple, striped channels represent voltage-gated calcium channels. ‘Synaptotagmin’ by
Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC-
BY-NC-SA) 4.0 International License. View static image of animation
Step 4: Release of neurotransmitters into the

synapse
The last step of neurotransmitter release is the fusing of the cell membrane. In order to release their
chemical contents into the synapse, vesicles need to fuse with the cell membrane. As the vesicular
membrane merges with the interior of the neuronal membrane, the membranes fuse and the contents
of the vesicle become exposed to the extracellular space. The neurotransmitters then float across the
aqueous synapse, giving them the opportunity to interact with postsynaptic receptors.
Animation 12.4. Once the synaptotagmin-SNARE protein complex forms, the synaptic vesicle
membrane fuses with the terminal membrane, and the neurotransmitters are released into the synaptic
cleft through exocytosis. The purple, striped channels represent voltage-gated calcium channels.
‘Transmitter Exocytosis’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Step 5: Receptor Activation

After exocytosis of the transmitter molecules, neurotransmitters traverse across the synapse and bind
to receptors on the postsynaptic membrane. Receptors fall into two main categories: ionotropic
receptors (also called ligand-gated channels) and metabotropic receptors (also called G-protein
coupled receptors). These two types of receptors will be covered in upcoming chapters.
Figure 12.4. After exocytosis of the neurotransmitters into the synaptic cleft, the transmitters bind to
receptors present on the postsynaptic membrane. ‘Neurotransmitter in Synapse’ by Casey Henley is
Step 6: Postsynaptic potentials generated in

postsynaptic cell summate
Recall the process of summation from Chapter 8. The postsynaptic potentials generated from
neurotransmitter binding summate at the axon hillock. If the membrane potential is over threshold
potential for the cell, then a new action potential will be generated in the postsynaptic cell.
Postsynaptic potentials (graded potentials) can occur following different types of stimulation.
When neurotransmitters bind to receptors on postsynaptic cells, they cause the receptor proteins to
undergo a conformational change, and open ligand-gated ion channels that produce depolarization or
hyperpolarization.
Figure 12.5. EPSPs can summate via temporal or spatial summation. Temporal summation occurs
when a presynaptic neuron (Input 1 in the figure) stimulates the postsynaptic neuron multiple times
in a row. Spatial summation occurs when more than one presynaptic neuron (Inputs 1 through 4 in
the figure) each stimulate the postsynaptic neuron at the same time. The EPSPs of each stimulation
will add together to cause a stronger depolarization of the membrane potential of the postsynaptic
neuron than one excitatory stimulus alone. ‘Synaptic Summation’ by Casey Henley is licensed under
License.
Step 7: Neurotransmitter Inactivation

It is important that neurotransmitter signaling be tightly regulated. This means that there needs to be
ways to terminate chemical signaling. Inactivation can be accomplished in three different ways.
1. After being released, neurotransmitters can be altered into inactive substances.

2. Neurotransmitters can go through the reuptake process, where they are recycled by being
transported back into the presynaptic cell and repackaged into synaptic vesicles.
3. Some neurotransmitters simply float away from the synapse due to the aqueous environment
surrounding neurons.
Key Takeaways
• In order for neurotransmitter release to be initiated, an action potential (depolarizing

stimulus) needs to arrive at the axon terminal to open voltage-gated calcium
channels
• Neurotransmitter release is dependent on the influx of calcium into the terminal
through voltage-gated calcium channels
• SNARE proteins are important for vesicle docking at active zones and exocytosis of
neurotransmitters into the synapse
• Synaptotagmin is a calcium sensor
• Neurotransmitters bind to postsynaptic receptors and cause postsynaptic potentials
that summate within the postsynaptic cell
• Neurotransmitter signaling is terminated through chemical inactivation, reuptake, or
through diffusion away from the synapse.
Test Yourself!
Attributions
150 | NEUROTRANSMITTER IDENTIFICATION AND ACTION: IONOTROPIC RECEPTORS VERSUS
METABOTROPIC RECEPTORS
13.
NEUROTRANSMITTER IDENTIFICATION
AND ACTION: IONOTROPIC RECEPTORS
VERSUS METABOTROPIC RECEPTORS
Neurotransmitters are chemicals that are released at the

synapse. In this chapter, we will introduce basic
Resources information about neurotransmitters and how they are
identified. Next, an overview of neurotransmitter
receptors will be provided.
Receptors are proteins located on the postsynaptic cell
• Glossary Terms
that are capable of sending a signal to change the function
• Key Takeaways
or activity of the postsynaptic neuron. Most receptors
• Test Yourself
that function in neurotransmission are large
transmembrane proteins. On the extracellular surface of
the protein is a specific series of amino acid residues called
the active site. The active site, also called the orthosteric site, is shaped to allow molecules of
neurotransmitter to bind to the receptor. Receptors are classified into one of two main categories:
Ionotropic receptors and Metabotropic receptors (G-protein coupled receptors).
Neurotransmitters
Neurotransmitters are the chemicals released at the presynaptic terminal that then bind to the
postsynaptic cell. A neurotransmitter system is the neurotransmitter and everything needed for the
synthesis of the transmitter, the packaging of the transmitter into vesicles, the proteins necessary for
reuptake of the transmitter into the presynaptic cell, degradation of the transmitter, and postsynaptic
NEUROTRANSMITTER IDENTIFICATION AND ACTION: IONOTROPIC RECEPTORS VERSUS METABOTROPIC
RECEPTORS | 151
signaling of the transmitter. In the following chapters, we will discuss various neurotransmitter
systems.
Though there are neurons that can release more than one type of neurotransmitter (referred to
as co-transmitters), most neurons release just one type of neurotransmitter, allowing neurons to
be identified by the neurotransmitter they release (e.g. dopaminergic neurons). The different
neurotransmitters can be separated into three different chemical categories:
1. Amino Acids (Glutamate, GABA, Glycine)

2. Amines (Acetylcholine, Dopamine, Epinephrine, Histamine, Norepinephrine, Serotonin)
3. Peptides (Dynorphin, Enkephalin, Substance P, Neuropeptide Y)
To identify whether a chemical can be classified as a neurotransmitter, it must meet the following
requirements:
1. The chemical has to be stored or located within the presynaptic neuron. Both
immunohistochemistry and immunocytochemistry can use antibodies directed against a specific
protein important for neurotransmitter synthesis or storage to localize the protein to a neuron
and determine whether a neuron has the appropriate machinery to make the neurotransmitter
and thus likely release the neurotransmitter.
2. The chemical has to be released from the presynaptic neuron following appropriate stimulation.
3. When the chemical is applied to the postsynaptic cell by an experimenter, the response of the
postsynaptic cell should be similar to the response following normal release from the presynaptic
cell.
Neurotransmitters can be classified on their function as either ‘excitatory’ or ‘inhibitory’. In the

following chapters, we will see that glutamate is an example of an excitatory neurotransmitter. This is
because the binding of glutamate to the postsynaptic cell typically generates excitatory postsynaptic
potentials, which makes the inside of the cell closer to zero and thus closer to threshold potential
for the cell, increasing the likelihood of firing an action potential. GABA and Glycine, however, are
typically considered as inhibitory neurotransmitters. This is because the binding of either GABA or
glycine to the postsynaptic cell typically generates inhibitory postsynaptic potentials, which makes
the inside of the cell more negative and further from threshold potential of the cell, decreasing the
likelihood of firing an action potential.
Most neurotransmitters, however, can produced either excitatory postsynaptic potentials or

inhibitory postsynaptic potentials depending on the properties of the postsynaptic receptor that they
bind to. Neurotransmitters each bind to specific receptors that produce specific postsynaptic
responses.
The subsequent chapters will review the main neurotransmitters including their synthesis, storage,
and their receptors.
Neurotransmitter Receptors
Ionotropic Receptors
Ionotropic receptors are also called neurotransmitter-gated or ligand-gated channels. They are ion
channels that open in response to the binding of a neurotransmitter. They are primarily located along
the dendrites or cell body, but they can be present anywhere along the neuron if there is a synapse.
Ionotropic receptors are important for receiving incoming information from other neurons.
RECEPTORS | 153
Figure 13.1. Ligand-gated channels critical for receiving incoming synaptic information are primarily
located along the dendrites and cell body. ‘Receptor Location’ by Casey Henley is licensed under a
License.
Physically, ionotropic receptors are transmembrane proteins with a large-diameter pore through which
ions can pass. Although ionotropic receptors are ion channels, they open in a different way than the
voltage-gated ion channels needed for propagation of the action potential. The ionotropic receptors
are ligand-gated, which means that a specific molecule, such as a neurotransmitter, must bind to the
receptor to cause the channel to open and allow ion flow. As seen in previous chapters, the voltage-
gated channels open in response to the membrane potential reaching threshold (a specific voltage).
Ionotopic channels are often said to use a direct signaling mechanism because the
neurotransmitter binds directly to the ion channel that it is going to open.
Animation 13.1. Ionotropic receptors, also called ligand-gated channels, are ion channels that are
opened by the binding of neurotransmitters. Voltage-gated channels are opened by the membrane
potential of the cell reaching threshold. Both types of channels allow ions to diffuse down their
electrochemical gradient. The lined, teal channels represent glutamate receptors; the solid yellow
channels represent GABA receptors; the dotted, blue channels represent voltage-gated sodium
channels. ‘Ion Channel Gating’ by Casey Henley is licensed under a Creative Commons Attribution
Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License. View static image of
animation.
These channels only open when a specific ligand binds to the active site on the extracellular side
of the protein. Neurotransmitters and receptors fit together like a lock and key; only certain
neurotransmitters are able to bind to and open certain receptors.
Once a neurotransmitter activates the ionotropic receptor, ions will move through the channel
based on the electrochemical gradient for that ion. As a result of ion movement, the cell’s membrane
potential will change. For example, if the ionotropic channel allowed for movement of sodium, the
electrochemical gradient for sodium will promote sodium rushing into the cell, causing the inside of
the cell to get more positive (decrease in membrane potential).
Ionotropic receptors are able to induce a change in membrane potential very rapidly, on the scale
of milliseconds. Due to the nature of the amino acid residues that make up the pore of ionotropic
receptors, they can be very selective for certain ions. For example, negatively charged residues lining the
–
inside of the pore repel negatively charged Cl ions while allowing positively charged cations to pass
through the channel.
RECEPTORS | 155
Animation 13.2. Since neurotransmitter receptors can only bind specific neurotransmitters, glutamate
binds to and opens glutamate receptors, but has no effect on GABA receptors. The lined, teal channels
represent glutamate receptors; the solid yellow channels represent GABA receptors. ‘Ligand and
Receptor’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Metabotropic Receptors (G-protein-coupled receptors)

Metabotropic receptors (also called G-protein-coupled receptors [GPCRs]), are membrane-bound
proteins. These receptor complexes cause the cell to change its metabolism in a way that leads to either
excitation or inhibition of the postsynaptic cell. Like ionotropic receptors, metabotropic receptors are
primarily located along the dendrites or cell body, but they can be present anywhere along the neuron
if there is a synapse.
Unlike ionotropic receptors (direct mechanism), ions do not pass through GPCRs. Instead,
metabotropic receptors use the actions of G proteins, proteins which induce changes in neuronal
excitability through the action of second messenger signaling molecules. Due to these extra steps,
GPCRs have slower effects than ionotropic receptors, but they can have long-lasting effects, unlike the
brief action of a postsynaptic potential.
G-Proteins
G-proteins are enzymes with three subunits: alpha, beta, and gamma. There are multiple types of alpha
subunits (Gαs, Gαi, Gαq), and each initiate different cellular cascades in the neuron.
Functionally speaking, these G-proteins are capable of binding to molecules of guanosine
triphosphate (GTP) or guanosine diphosphate (GDP). Chemically similar to ATP, GTP can function
as a source of energy. G-proteins themselves exhibit catalytic activity of GTP. This means that they are
capable of breaking down GTP into the less-energetic GDP. When GTP is bound to the GPCR, the
receptor is active. When this molecule is hydrolyzed into GDP, the receptor becomes inactive.
Figure 13.2. The unactivated G-protein complex in the cell consists of three subunits (alpha, beta,
and gamma) and a bound GDP molecule. ‘G-protein Complex’ by Casey Henley is licensed under
License.
Metabotropic Receptors
A metabotropic receptor or GPCR is a transmembrane protein that has an extracellular binding site
for a neurotransmitter. Metabotropic receptors are physically linked to G-proteins, which exist on the
inner surface of the cell membrane.
When a neurotransmitter binds to a GPCR it undergoes a conformational change, which allows the
receptor to interact with an associated inactivated G-protein complex.
The complex that binds is specific to the receptor; different metabotropic receptors for the same
neurotransmitter can have different effects in the cell due to which G-protein binds. Once coupled
to the receptor, the GDP molecule is exchanged for a GTP molecule and the G-protein becomes
activated.
Animation 13.3. Neurotransmitter binding to a G-protein-coupled receptor causes the inactivated G-

protein complex to interact with the receptor. The GDP molecule is then exchanged for a GTP molecule,
which activates the G-protein complex. ‘G-protein Binding’ by Casey Henley is licensed under a Creative
Commons Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0 International License. View
RECEPTORS | 157
After activation, the G-protein complex will separate into the alpha-GTP subunit and the beta-
gamma subunit. Both components can alter the function of effector proteins in the cell. Effector
protein functions can range from altering ion permeability across the membrane by opening ion
channels to initiating second messenger cascades. Second messenger cascades can have long-term,
widespread, and diverse cellular effects including activation of cellular enzymes or altering gene
transcription.
Animation 13.4. Once activated, the G-protein complex will separate into the alpha-GTP subunit and
the beta-gamma subunit. These subunits can stimulate or inhibit effector proteins within the cell. ‘G-
protein Effects’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Share-Alike (CC BY-NC-SA) 4.0 International License. View static image of anim ation.
Adenylyl Cyclase / cAMP Second Messenger Cascade
The cyclic AMP (cAMP) second messenger pathway is used by many GPCRs. When a
neurotransmitter binds to a GPCR, it causes activation of the Gαs alpha subunit. The Gαs subunit
(“s” for stimulatory) translocates within the cell to stimulate an effector enzyme called adenylyl
cyclase. When activated, adenylyl cyclase converts ATP to cAMP in the cytoplasm. cAMP is
considered a 2nd messenger (the 1st messenger was the neurotransmitter). As a second messenger,
cAMP has the ability to alter proteins inside the cell. Specifically, cAMP activates another enzyme
called protein kinase A (PKA) by binding to the regulatory subunits, allowing the catalytic
(functional) subunits to separate and become active. PKA is a kinase, a group of enzymes that add a
phosphate molecule to proteins, a mechanism called phosphorylation. The addition of the phosphate
changes the activity of the protein and how it functions in the cell. Typically, phosphorylation activates
proteins within the cell.
Animation 13.5. GPCRs that couple to the Gs alpha subunit initiate the adenylyl cyclase / cAMP
pathway. The Gs subunit activates adenylyl cyclase, which then converts ATP to cAMP. cAMP binds
to and activates protein kinase A (PKA), which phosphorylates proteins in the cell. ‘Adenylyl Cyclase
Pathway’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-
Alike (CC BY-NC-SA) 4.0 International License. View static image of animation.
The end effects of this pathway will depend on which proteins are targeted. For example, cAMP
can gate ion channels and PKA can phosphorylate ion channels altering permeability and membrane
potential. Phosphorylation can open the channel, or it may modulate the activity of the channel,
making the channel easier to open or remain open longer.
Animation 13.6. The adenylyl cyclase / cAMP pathway can alter many cellular functions. One example
is that both cAMP and PKA can open ion channels. Like ligand-gated channels, there are also cAMP-
gated channels, which open after cAMP binding. PKA is able to phosphorylate and modulate ion channel
function by converting ATP to ADP. ‘Second Messenger Ion Channel Action’ by Casey Henley is licensed
under a Creative Commons Attribution Non-Commercial Share-Alike (CC BY-NC-SA) 4.0
In addition to altering ion channel function, PKA can phosphorylate other proteins important for
RECEPTORS | 159
neuron function, such as proteins involved with neurotransmitter synthesis and release. One other
critical target of PKA phosphorylation is the transcription factor CREB (cAMP response element-
binding protein). Transcription factors bind to DNA in the nucleus and change the rate of gene
transcription. Phosphorylation by PKA can cause CREB to initiate transcription of genes, creating
new proteins for the neuron. Depending on which genes are transcribed, the effects on the neuron can
be long-lasting.
Overall, neurotransmitters working through GPCRs and second messenger cascades (like the
adenylyl cyclase pathway) can cause a diverse range of cellular effects: from opening ion channels, to
changing protein activity via phosphorylation, to altering the proteins synthesized in the neuron.
Animation 13.7. PKA can phosphorylate a number of proteins involved with neuron function. It can
target proteins involved with neurotransmitter synthesis, packing, and release, or it can enter the nucleus
and phosphorylate CREB, a transcription factor that can initiate gene transcription and protein synthesis.
‘PKA Targets’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Share-Alike (CC BY-NC-SA) 4.0 International License. View static image of animation.
Alternatively, a GPCR that is coupled with Gαi causes a decrease in excitability. In many ways,
Gαi proteins serve the opposite function as Gαs proteins—the “i” stands for inhibitory. Whereas
activation of Gαs increases the action of adenylyl cyclase, Gαi proteins decrease adenylyl cyclase activity.
Therefore, Gαi activation decreases the intracellular concentration of cAMP, in turn decreasing PKA
activity. Given the function of PKA as a kinase that increases cellular excitation as described above, a
GPCR coupled to Gαi that causes decreased PKA activity inhibits cellular activity through multiple
mechanisms, some of which include decreased current through receptors, decreased trafficking of
receptors to the presynaptic neuronal membrane, and decreased transcription of certain genes.
Phosphlipase C / IP3 / DAG Second Messenger Cascade
Generally, Gαq is an excitatory G-protein alpha subunit. The Gαq subunit initiates a separate signaling
pathway in the cell by activating the effector enzyme phospholipase C. Phospholipase C targets PIP2
(phosphatidylinositol 4,5-bisphosphate), which is a phospholipid present in the plasma membrane of
the cell. PLC will split PIP2 into two separate second messenger molecules: the soluble IP3 (inositol
1,4,5-trisphosphate) and membrane-embedded DAG (diacylglycerol).
Animation 13.8. The Gq G-protein subunit activates phospholipase C, which converts the phospholipid
PIP2 in the cell membrane into DAG, another membrane-bound molecule, and IP3, a cytoplasmic
molecule. DAG can interact with PKA, initiating phosphorylation of cellular proteins. IP3 opens calcium
channels in the endoplasmic reticulum, allowing calcium to flow into the cytoplasm. Calcium, another
second messenger can have many cellular effects. It can bind to calmodulin, which then activates CaMK,
causing phosphorylation of more protein targets. ‘IP3-DAG Pathway’ by Casey Henley is licensed under
DAG remains in the membrane and interacts with protein kinase C (PKC). PKC is an enzyme
that can act to increase neurotransmitter release probability.
One function of IP3 is to move to the endoplasmic reticulum, where it opens calcium channels
embedded in the endoplasmic reticulum and allows calcium to flow into the cytosol, elevating
intracellular calcium levels. This increase in calcium concentration can depolarize the cell and activate
calcium-dependent processes, which often lead to cellular excitation.
Calcium also acts as a second messenger in the cell. One important effect is the binding of calcium
to the calmodulin protein. This complex can then activate another kinase, the calcium/calmodulin-
dependent protein kinase (CaMK). Both PKC and CaMK can phosphorylate specific cellular and
nuclear proteins like PKA.
So, depending on the type of Gα subunit that the G-protein is associated with, there will be
different outcomes for the cell. In general, Gαs will “stimulate” the cell by activating adenylyl cyclase.
Gαi will “inhibit” the cell by inhibiting adenylyl cyclase. Gαq will “stimulate” the cell by activating
phospholipase C.
RECEPTORS | 161
Figure 13.3. The second messenger pathway used, and whether that pathway is stimulated or
inhibited, depends on the type of alpha subunit in the G-protein complex. Different receptors couple
to different G-protein complexes. This allows one neurotransmitter to initiate multiple types of
signaling cascades. A) The norepinephrine beta-adrenergic receptor couples to the Gs subunit and
activates adenylyl cyclase, which initiates downstream cellular effects. B) The norepinephrine alpha
2-adrenergic receptor couples to the Gi subunit and inhibits adenylyl cyclase, which prevents
RECEPTORS | 163
downstream cellular effects. C) The norepinephrine alpha 1-adrenergic receptor couples to the
Gq subunit and activates phospholipase C, which initiates downstream cellular effects. ‘Alpha
Subunit Effects’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Signal Amplification
One characteristic of GPCR activation is the signal amplification that takes place. One receptor is
able to activate more than one G-protein complex. The effector protein activated by the G-protein
can create many second messengers, and the activated protein kinases can each phosphorylate multiple
cellular proteins. This means that one neurotransmitter can have a significant effect on cellular
function.
Figure 12.4. The second messenger cascades initiated by GPCRs undergo significant signal
amplification. A) Multiple G-proteins can be activated by a GPCR. B) Each effector protein is able to
synthesize numerous second messenger molecules. C) Each protein kinase activated by the second
messengers can phosphorylate various cellular proteins. ‘Signal Amplification’ by Casey Henley is
RECEPTORS | 165
Signal Termination
Eventually, the cascade initiated by binding of the neurotransmitter to the GPCR needs to end. The
alpha subunit of the G-protein is able to convert the bound GTP back to GDP after a short period
of time, inactivating the G-protein. The alpha subunit will then interact with a beta-gamma subunit
and stay in the resting state until activated by another GPCR. Enzymes in the cell called protein
phosphatases find and remove the phosphate groups added to cellular proteins by the protein kinases.
Finally, other cellular mechanisms exist to remove calcium from the cytoplasm and degrade other
second messengers.
Key Takeaways
• Ionotropic receptors are ligand-gated ion channels that open when a specific
neurotransmitter binds
• Ionotropic receptors represent a direct mechanism to alter the postsynaptic cell
because the neurotransmitter binds to the ion channel directly
• Metabotropic receptors represent an indirect mechanisms to alter the postsynaptic
cell because the neurotransmitters binds to a separate protein from the effector
proteins that alter the postsynaptic cell
• G-protein-coupled receptors rely on the activation of G-proteins to cause cellular
changes
• G-protein-coupled receptors have slower effects than ligand-gated receptors
• G-proteins can open ion channels, alter protein function via phosphorylation, and
alter gene transcription
• The Gs subunit initiates the adenylyl cyclase / cAMP signaling pathway
• The Gi subunit inhibits the adenylyl cyclase / cAMP signaling pathway
• The Gq subunit initiates the phospholipase C / IP3 / DAG signaling pathway
Test Yourself!
Attributions
INTRODUCTION TO SMALL MOLECULE NEUROTRANSMITTERS | 167
14.
INTRODUCTION TO SMALL MOLECULE

NEUROTRANSMITTERS
Neurotransmitters are the substances that are released at

chemical synapses, and they are the signaling molecules
that allow neurons to communicate with one another. To Resources
date, scientists have identified more than 100
neurotransmitters.
A few criteria must be met for a molecule to be called a
• Key Takeaways
neurotransmitter. First, the neurotransmitter must be
• Test Yourself
synthesized within the presynaptic neuron. Second, the
neurotransmitter must be released by the presynaptic
neuron in response to stimulation. Third, when a
postsynaptic neuron is treated with the neurotransmitter by a researcher, the molecule must cause the
same effect in the postsynaptic neuron as when it is released by a presynaptic neuron.
The next several chapters will cover a subset of neurotransmitter systems. For each neurotransmitter
system, we will provide information on: the synthesis and storage of the neurotransmitter in the
presynaptic cell; the receptors that the neurotransmitter binds to on the postsynaptic cell and the
postsynaptic effects; and how neurotransmitter signaling is terminated.
Neurotransmitter Vesicles: Types of Vesicles

There are two main categories of neurotransmitters: small molecule neurotransmitters and peptide
neurotransmitters (covered in Chapter 19). Synthesis and storage of these neurotransmitter groups
differ. Small molecule neurotransmitters are synthesized and stored in the terminal for fast release.
Neuropeptides are synthesized in the cell body and must be transported to the terminal, which can
168 | INTRODUCTION TO SMALL MOLECULE NEUROTRANSMITTERS
lead to slower release. Additionally, a neuron typically will synthesize and release only one type of small
molecule neurotransmitter, but can synthesize and release more than one neuropeptide.
Molecules of neurotransmitters are often stored in synaptic vesicles before being released. Synaptic
vesicles are tiny spheres of neurotransmitter enclosed by a phospholipid bilayer just like the cell
membrane. These vesicles can be roughly characterized into two classes:
1. Small vesicles. Small vesicles are responsible for storage of small molecule neurotransmitters.
Given the size of neurotransmitters, we can estimate that thousands to tens of thousands of
molecules of neurotransmitter can be stored in each vesicle. Small vesicles store many of the
neurotransmitters we most often think of, including glutamate, GABA, dopamine, and
norepinephrine. Small vesicles are almost always exclusively found in the axon terminals.
2. Large dense-core vesicles. These vesicles are much larger than small vesicles. They store
peptides such as dynorphin or enkephalin, which have chemical structures much larger than the
small molecule neurotransmitters. Since these peptides are packaged into their vesicles near the
nucleus, large dense-core vesicles can be found in the cell bodies and all along the axons in
addition to the axon terminal.
Small Molecule Transmitters

Let’s first discuss the small molecule neurotransmitters. The small molecule neurotransmitters can be
divided into two main groups: amino acid neurotransmitters and biogenic amines (also called
monoamines). In addition to acting as neurotransmitters, the amino acids glutamate and glycine are
used to synthesize proteins in all cell types throughout the body. GABA (Ɣ-Aminobutyric acid) is a
metabolite of glutamate, but is not used in protein synthesis in the body. The biogenic amines include
serotonin, histamine, and the subgroup catecholamines: dopamine, norepinephrine, and epinephrine.
Acetylcholine does not fit into either division but is still considered a small molecule neurotransmitter.
Most small molecule neurotransmitters are synthesized by enzymes that are located in the cytoplasm
(the exception is norepinephrine, see Chapter 17). This means that small molecule neurotransmitters
can be synthesized and packaged for storage in the presynaptic terminal using enzymes present in the
terminal.
Figure 14.1. Small

molecule
neurotransmitter
s can be
subdivided into
groups based on
chemical
structure. Amino
acid transmitters
include
glutamate,
GABA, and
glycine. The
biogenic amines
include serotonin
and histamine,
and the
catecholamines, a
subgroup of the
biogenic amines,
include
dopamine,
norepinephrine,
and epinephrine.
Acetylcholine
does not fit into
a group. ‘Small
Molecule
Neurotransmitter
s’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
The effect of the neurotransmitter is dependent

170 | INTRODUCTION TO SMALL MOLECULE NEUROTRANSMITTERS
on the properties of the postsynaptic receptor

In the last chapter, the mechanisms of ionotropic and metabotropic (GPCRs) were covered. In the
chapters that follow, we will focus on a subset of neurotransmitter systems and identify the different
neurotransmitter receptors and their function.
Key Takeaways
• Small molecules neurotransmitters are synthesized and packaged into vesicles in the
presynaptic terminal
• There are different types of vesicles that hold small molecule neurotransmitters
versus neuropeptide neurotransmitters
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Attributions

172 | NEUROTRANSMITTERS: ACETYLCHOLINE
15.
NEUROTRANSMITTERS:
ACETYLCHOLINE
Acetylcholine was the first neurotransmitter discovered

and chemically isolated, a feat which earned two
researchers the shared Nobel Prize in Physiology or
Resources Medicine in 1936. One of the two scientists, a German
pharmacologist named Otto Loewi, stimulated the vagus
nerve connected to an isolated frog heart, which caused
the heart rate to slow down. When he applied the
• Scientist Links to Learn surrounding solution from this experiment to a second
More heart, he observed that the second heart also slowed down,
• Glossary Terms despite having no physical connection to the first heart.
• Key Takeaways From this, he concluded that a chemical was being
• Test Yourself released by the vagus nerve that was decreasing the heart
rate. This chemical was first called Vagusstoff, the German
word meaning Vagus substance. Today, we know it as
acetylcholine.
NEUROTRANSMITTERS: ACETYLCHOLINE | 173
Figure 15.1. Otto

Loewi
Experiment. The
vagus nerve that
served an
isolated frog
heart was
stimulated and
the contractile
force of the heart
was measured.
Stimulation of
the vagus nerve
resulted in a
slowed heart
rate. When the
solution
surrounding the
heart was
removed and
applied to a
second separate
frog heart, that
second heart also
showed a slowed
heart rate. ‘Otto
Loewi
Experiment’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Acetylcholine Synthesis and Storage

Acetylcholine (ACh) is a small molecule neurotransmitter best known for its role at the
neuromuscular junction, the synapse between a motor neuron and a muscle fiber. In the presynaptic
terminal, acetylcholine is synthesized from acetyl coenzyme A (acetyl CoA) and choline via the
enzyme choline acetyltransferase (ChAT). The level of enzyme activity is the rate-limiting step in the
synthesis pathway. The presence of ChAT in a neuron is used as a biochemical marker for neurons that
produce acetylcholine. Acetylcholine is packaged into small vesicles for storage in the terminal via the
vesicular acetylcholine transporter (VAChT), a protein found in the synaptic vesicle membrane.
Figure 9.2.
Acetylcholine is
synthesized from
acetyl CoA and
choline by
choline
acetyltransferase,
the rate-limiting
step in the
pathway.
Acetylcholine is
then packaged
into vesicles by
vesicular
acetylcholine
transporter.
‘Acetylcholine
Synthesis’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Acetylcholine Receptors
Nicotinic Acetylcholine Receptors

Acetylcholine is able to act at both ionotropic and metabotropic receptors, and activity at both
receptor classes is essential for normal function. The ionotropic receptors of the nervous system are
called nicotinic acetylcholine receptors because they can be activated by nicotine in addition to
acetylcholine. Nicotinic receptors are located primarily outside of the central nervous system in the
periphery. Specifically, the nicotinic receptors are used at the neuromuscular junction, which is the
junction of a motor neuron and a skeletal muscle. Acetylcholine is released by motor neurons, where
it activates nicotinic acetylcholine receptors on skeletal muscle cells. This excites the muscle cells and
causes them to contract.
Nicotinic acetylcholine receptors are non-selective cation channels. The channel is closed when
acetylcholine is not bound to the receptor. The nicotinic receptor has two binding sites for
acetylcholine. When acetylcholine is bound to both sites on the receptor, the pore of the channel
opens to allow movement of both sodium and potassium. This ionotropic receptor is an example of a
direct mechanism of action, due to the fact that the receptor and the channel are located on the same
protein. The properties of the receptors cause more sodium to enter than potassium leaves, ultimately
causing the membrane to depolarize. Thus, nicotinic receptors are always excitatory (produce EPSPs)
and cause depolarization of the postsynaptic cell.
Figure 15.3.
Nicotinic
Acetylcholine
Receptor. The
nicotinic
acetylcholine
receptor is an
ionotropic
receptor.
Without
acetylcholine
bound, the
nicotinic receptor
is inactive and in
a closed state
(left). When two
molecules of
acetylcholine
(shown as blue
circles) are bound
to the receptor,
the channel pore
opens and allows
for movement of
+
potassium (K )
out of the cell
and also
movement of
+
sodium (Na )
into the cell. The
movement of
sodium (Na+)
dominates,
leading to
depolarization or
excitatory
postsynaptic
potentials in the
postsynaptic cell
(right).
Muscarinic Receptors
On the other hand, muscarinic acetylcholine receptors are GPCRs (or metabotropic receptors) and
utilize an indirect mechanism of action. When acetylcholine binds to muscarinic receptors, the beta-
gamma subunit of the G-protein complex translocates in the cell to affect potassium channels in the
cell membrane, causing them to open. Due to the electrochemical gradient of potassium, potassium
will leave the cell and cause membrane hyperpolarization, or IPSPs.
Muscarinic receptors are located in the heart, and their activation causes a decrease in heart rate due
to this hyperpolarization caused by the muscarinic receptors (as Otto Loewi demonstrated with the
isolated frog heart preparation.)
Animation 15.1. Some GPCRs, like the muscarinic acetylcholine receptors in the heart, alter cellular
permeability by opening ion channels. The activated beta-gamma subunit of the muscarinic receptor
opens GIRK potassium channels and allows the efflux of potassium. ‘Beta-Gamma Ion Channels’ by
Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC
BY-NC-SA) 4.0 International License. View static image of animation.
Neuropharmacology and Acetylcholine Systems

Different drugs have the ability to interact within a neurotransmitter system, in many cases by directly
interacting with a neurotransmitter receptor protein. There are two main classes of drugs that we will
discuss: agonists and antagonists. An agonist is a drug that binds to a receptor and acts to activate
the normal function of that receptor. An antagonist is a drug that binds to a receptor that acts to
block the normal function of that receptor. The acetylcholine neurotransmitter system can be used as
an example to see the function of both agonists and antagonists.
Drugs that interact with the nicotinic acetylcholine

receptor
Nicotine is an agonist for the nicotinic acetylcholine receptor (which is how the receptor got its
name). Normally, the nicotinic receptor causes excitatory postsynaptic potentials. Because nicotine
is an agonist for this receptor, when it binds to the nicotinic acetylcholine receptor it also causes
excitatory post synaptic potentials and lead to an overall increase in activity.
There are also antagonists for the nicotinic acetylcholine receptor. Curare, a substance that comes
from the resin of a tree in South America and α-bungarotoxin, a toxin found in Krait snake venom,
are both examples of antagonists for the nicotinic acetylcholine receptor. Recall that normally the
nicotinic acetylcholine receptor causes excitatory post synaptic potentials. As antagonists, curare and
α-bungarotoxin will block excitatory postsynaptic potentials, leading to an overall decrease in activity.
Drugs that interact with the muscarinic receptor

Muscarine, a substance found in certain mushrooms, is an agonist for the muscarinic receptor (and
how the receptor got its name). As an agonist, muscarine will promote the normal function of the
receptor. Normally, muscarinic receptors produce inhibitory post synaptic potentials. Thus,
muscarine binding to muscarinic receptor will cause inhibitory post synaptic potentials, causing an
overall decrease in activity.
Atropine is a drug that acts as an antagonist at the muscarinic receptor. Antagonists block the
normal function of the receptor. Because normally the muscarinic receptor is inhibitory, atropine will
block the normal inhibitory effects, leading to an overall increase in activity.
Termination of Acetylcholine Signaling

The enzyme acetylcholinesterase, located in the synaptic cleft and within the postsynaptic
membrane, chemically breaks down acetylcholine into acetic acid and choline.
Choline can also undergo the process of reuptake which will remove choline from the synapse
(which will effectively stop acetylcholine signaling) and bring it into the presynaptic cell through the
choline transporter. Acetylcholinesterase is an important enzyme to regulate acetylcholine within
the body. Recall that acetylcholine is used at the neuromuscular junction between motor neurons and
skeletal muscles. The acetylcholinesterase enzyme prevents continuous stimulation of our muscles,
allowing tight control. In fact, acetylcholinesterase is one of the fastest acting enzymes within the
body. Nerve gas and neostigmine (in Nigerian beans) both act to inhibit acetylcholinesterase, causing
excessive acetylcholine signaling.
Figure 15.4. Acetylcholine is degraded into choline and acetate within the synaptic cleft via
acetylcholinesterase. Choline is then transported back into the presynaptic terminal. ‘Acetylcholine
Degradation’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Key Takeaways
• Acetylcholine was the first identified neurotransmitter and was discovered by Otto
Loewi
• Acetylcholine is synthesized within the presynaptic terminal from choline and acetate
through ChAT and packaged into vesicles by VAchT
• Acetylcholine binds to both ionotropic (nicotinic) and metabotropic (muscarinic)
receptors
• Nicotinic receptors are excitatory and muscarinic receptors are inhibitory
• Agonists are drugs that promote the normal activity of the receptor that they bind to
• Antagonists are drugs that block the normal activity of the receptor that they bind to
• Acetylcholine signaling is terminated through the enzymatic activity of
acetylcholinesterase
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Attributions
Media Attributions
• Acetylcholine Experiment © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

• Acetylcholine Synthesis © Casey Henley is licensed under a CC BY-SA (Attribution ShareAlike)
license
• Nicotinic Receptor © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-SA
182 | NEUROTRANSMITTERS: AMINO ACID NEUROTRANSMITTERS (GLUTAMATE, GABA, GLYCINE)
16.
NEUROTRANSMITTERS: AMINO ACID

NEUROTRANSMITTERS (GLUTAMATE,
GABA, GLYCINE)
Glutamate
Glutamate is an amino acid transmitter and is the primary
Resources
excitatory neurotransmitter in the brain. Glutamate is the
same as the amino acid glutamic acid. There is more
glutamate per volume of brain tissue than any other
neurotransmitter.
• Glossary Terms
In the presynaptic terminal, glutamine is converted
• Key Takeaways
into glutamate via the enzyme glutaminase, which is the
• Test Yourself
rate-limiting step in the synthesis pathway. Glutamate is
packaged into small vesicles for storage via the vesicular
glutamate transporter (vGLUT). Staining for the
presence of vGLUT is one way that researchers are able to identify glutamatergic neurons.
NEUROTRANSMITTERS: AMINO ACID NEUROTRANSMITTERS (GLUTAMATE, GABA, GLYCINE) | 183
Figure 16.1. Glutamate is synthesized from glutamine by glutaminase, the rate-limiting step in the
pathway. Glutamate is then packaged into vesicles by vesicular glutamate transporter. ‘Glutamate
Synthesis’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Glutamate Receptors
Glutamate can activate both ionotropic and metabotropic receptors. Glutamate is the primary
excitatory neurotransmitter in the central nervous system and opens non-selective cation channels.
There are three subtypes of ionotropic glutamate receptors: AMPA, kainate, and NMDA receptors.
The AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) and kainate receptors allow
both sodium and potassium to cross the membrane. Although potassium can leave the cell when
the receptors open, the electrochemical gradient driving sodium ion movement is stronger than the
gradient driving potassium movement, resulting in a depolarization of the membrane potential.

Animation 16.1. AMPA and kainate glutamate receptors are non-selective ion channels that allow
both sodium and potassium to flow across the membrane. When glutamate binds, sodium flows in
and potassium flows out. The lined, teal channel represent AMPA receptors; the checkered, teal channel
represents kainate receptors. ‘AMPA and Kainate’ by Casey Henley is licensed under a Creative
Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International License. View
The NMDA (N-methyl-D-aspartate) receptor requires the binding of glutamate to open, but it is
also dependent on voltage. When the membrane potential is below, at, or near rest, a magnesium ion
blocks the open NMDA receptor and prevents other ions from moving through the channel. Once the
cell depolarizes, the magnesium block is expelled from the receptor, which allows sodium, potassium,
and calcium to cross the membrane. The voltage change needed to open the NMDA receptor is usually
a result of AMPA receptor activation.
Animation 16.2. NMDA receptors are opened by a combination of glutamate binding and a voltage
trigger. At low levels of stimulation, when the the membrane potential is near rest, a magnesium ion
blocks the open NMDA receptor channel preventing ion flow. Ions can flow through open AMPA receptors,
which begins to depolarize the membrane. The voltage change eventually expels the magnesium ion from
the channel, allowing sodium, potassium, and calcium to cross the membrane. The lined, teal channel
represents AMPA receptors; the dotted, violet channel represents NMDA receptors. ‘AMPA and NMDA’
by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC-
BY-NC-SA) 4.0 International License. View static image of animation.
Termination of Glutamate Signaling

Glutamate action is terminated by two mechanisms. Reuptake of glutamate molecules into the
presynaptic terminal can occur, or glutamate can be transported into nearby glial cells. The
excitatory amino acid transporters are sodium co-transporters and use the sodium electrochemical
gradient to drive neurotransmitter transport. Within glial cells, glutamate is converted into glutamine
by glutamine synthetase. Glutamine is then transported out of the glial cell and back into the
presynaptic terminal for use in future glutamate synthesis. If glutamate is transported back into the
presynaptic terminal, it can be repackaged in synaptic vesicles.
Figure 16.2. Glutamine needs to removed from the synapse. The excitatory amino acid transporter
that uses sodium to drive glutamate movement across the membrane can move glutamate into glial
cells or back into the presynaptic terminal. In the terminal, glutamate is repackaged into synaptic
vesicles. In the glial cells, glutamate is broken down into glutamine by glutamine synthetase.
‘Glutamate Degradation’ by Casey Henley is licensed under a Creative Commons Attribution
GABA
Glutamate is then used to synthesize Gamma-aminobutyric acid (GABA), the main inhibitory
neurotransmitter in the brain. According to one estimate, about 25% of neurons in the brain are
GABAergic.
In the presynaptic terminal, glutamate is converted into GABA via the enzyme glutamic acid
decarboxylase (GAD), which—like the other synthesis pathways—is the rate-limiting step. GAD
is often used as a biochemical marker for the presence of GABAergic neurons. GABA is packaged
into small vesicles for storage in the terminal via the vesicular inhibitory amino acid transporter
(VIAAT).
Figure 16.3. GABA is synthesized from glutamate by glutamic acid decarboxylase, the rate-limiting
step in the pathway. GABA is then packaged into vesicles by vesicular inhibitory amino acid
transporter. ‘GABA Synthesis’ by Casey Henley is licensed under a Creative Commons Attribution
Glycine
Glycine is another inhibitory amino acid neurotransmitter, but unlike GABA, is mostly used by
neurons of the spinal cord and brain stem. Serine hydroxymethyltransferase converts the amino
acid serine into glycine in the presynaptic terminal. The rate-limiting step for glycine synthesis occurs
earlier in the pathway prior to serine synthesis. Glycine is packaged into small vesicles by the vesicular
inhibitory amino acid transporter (VIAAT) like GABA.
Figure 16.4. Glycine is synthesized from serine by serine hydroxymethyltransferase. Glycine is then
packaged into vesicles by vesicular inhibitory amino acid transporter. ‘Glycine Synthesis’ by Casey
GABA and Glycine Receptors

GABA and glycine receptors are chloride channels. Since an increase in chloride permeability across
the membrane is inhibitory, the binding of GABA or glycine to their respective ionotropic receptor
will cause inhibition.
Animation 16.3. GABA and glycine are inhibitory receptors that are selective to chloride. The solid yellow
channel represents a GABA receptor; the patterned, yellow channel represents a glycine receptor. ‘GABA
and Glycine’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Termination of GABA and Glycine Signaling
Like glutamate, GABA and glycine action are terminated by either reuptake into the presynaptic
terminal and packaging in synaptic vesicles or through transport into glial cells where breakdown can
occur. The GABA and glycine transporter also use the sodium electrochemical gradient to drive
the movement of the transmitter across the membrane.
Figure 16.5. GABA and glycine action is terminated by reuptake by sodium co-transporters into
either glial cells or back into the presynaptic terminal. In both locations, the neurotransmitters can
be broken down by enzymes, whereas in the presynaptic terminal, the transmitters can be
repackaged in synaptic vesicles. ‘GABA and Glycine Degradation’ by Casey Henley is licensed under
a Creative Commons Attribution Non-Commercial Share-Alike (CC-BY-NC-SA) 4.0 International
License.
Key Takeaways
• Glutamate is the primary excitatory neurotransmitter in the brain

• Glutamate binds to both ionotropic (AMPA, NMDA, and kainate) receptors and
metabotropic receptors
• GABA is the primary inhibitory neurotransmitter in the brain
• Glycine is the primary inhibitory neurotransmitter in the periphery
• GABA and Glycine bind to ionotropic receptors that open chloride channels
Test Yourself!
Attributions

NEUROTRANSMITTERS: BIOGENIC AMINES (SEROTONIN, HISTAMINE) | 191
17.
NEUROTRANSMITTERS: BIOGENIC
AMINES (SEROTONIN, HISTAMINE)
The biogenic amines encompass multiple

neurotransmitters, including serotonin, histamine,
dopamine, norepinephrine, and epinephrine. Included
within the biogenic amines is a separate group of Resources
neurotransmitters, the catecholamines.
This chapter will provide information for only
serotonin and histamine. Catecholamines are covered in
the following chapter. • Glossary Terms
• Key Takeaways
• Test Yourself
Serotonin
Serotonin, a biogenic amine neurotransmitter, is known
for its role in mood. As with dopamine (another monoamine neurotransmitter), there are only a few
areas of the brain that synthesize serotonin, the major one being the Raphe nucleus in the brain stem.
Synthesis
The enzyme tryptophan hydroxylase is the first step of serotonin biosynthesis, converting the amino
acid tryptophan into 5-hydroxytryptophan and is often used as a marker to identify serotonergic
neurons. This is also the rate-limiting step of the synthesis pathway. Then aromatic L-amino acid
decarboxylase converts the 5-hydroxytryptophan into serotonin. Serotonin is packaged into small
vesicles by the vesicular monoamine transporter similar to the other monoamine neurotransmitters:
dopamine, norepinephrine, and epinephrine.
192 | NEUROTRANSMITTERS: BIOGENIC AMINES (SEROTONIN, HISTAMINE)
Figure 18.1. Serotonin is synthesized in a two-step process. Tryptophan is converted into

5-hydroxytryptophan by tryptophan hydroxylase, the rate-limiting step in the pathway. Then serotonin
is synthesized from 5-hydroxytryptophan by aromatic L-amino acid decarboxylase. Serotonin is then
packaged into vesicles by vesicular monoamine transporter. ‘Serotonin Synthesis’ by Casey Henley is
Serotonin Receptors
Serotonin binds to many different classes of receptors, including both ionotropic and metabotropic
receptors. Most of the serotonin receptors are metabotropic receptors that utilize G-proteins to cause
the opening of ion channels, or alter the activity of phospholipase C or adenylyl cyclase. Due to the
large number of different serotonin receptors, the effects of serotonin can vary depending on the
properties of the postsynaptic receptor. Only one class of serotonin receptors are ligand-gated ion
channels and utilize a direct mechanism of action.
Termination of Serotonin Signaling

Like the other monoamines, serotonin goes through reuptake and is transported back into the
presynaptic terminal via the serotonin transporter (SERT). The difference between serotonin and
the catecholamines dopamine, norepinephrine, and epinephrine is that monoamine oxidase is the
only enzyme used for degradation.
Figure 18.3. Serotonin action is terminated by reuptake into the presynaptic terminal via SERT.
Serotonin is then either degraded by MAO or repackaged into synaptic vesicles. ‘Serotonin
Degradation’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Histamine
Finally, histamine is another biogenic amine transmitter that is synthesized from histidine through the
action of histamine decarboxylase, the rate-limiting step of the pathway. Like the other monoamine
neurotransmitters, it is packaged into small synaptic vesicles via the vesicular monoamine
transporter.
194 | NEUROTRANSMITTERS: BIOGENIC AMINES (SEROTONIN, HISTAMINE)
Though histamine has known roles in the immune system with allergic reactions and the
inflammatory response, this chemical can also act as a hormone and a neurotransmitter within the
central nervous system.
Figure 18.4. Histamine is synthesized from histadine by histadine decarboxylase, the rate-limiting step in
the pathway. Histamine is then packaged into vesicles by vesicular monoamine transporter. ‘Histamine
Synthesis’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-
Alike (CC BY-NC-SA) 4.0 International License.
Key Takeaways
• Serotonin and histamine are both examples of biogenic amine neurotransmitters

• Serotonin and histamine have different synthesis pathways, but are both packaged
into vesicles by the same protein, VMAT
• Serotonin binds to both ionotropic and metabotropic receptors
Test Yourself!
Attributions
196 | NEUROTRANSMITTERS: CATECHOLAMINES (DOPAMINE, NOREPINEPHRINE, EPINEPHRINE)
18.
NEUROTRANSMITTERS:
CATECHOLAMINES (DOPAMINE,
NOREPINEPHRINE, EPINEPHRINE)
Catecholamines are a class of neurotransmitters that are

found within the larger class of neurotransmitters,
biogenic amines. The catecholamines share many
Resources characteristics.
Dopamine
• Glossary Terms
• Key Takeaways Dopamine, a catecholamine transmitter, plays many roles
• Test Yourself in the nervous system, but it is best known for its roles in
reward and movement.
Synthesis
In the presynaptic terminal, the amino acid tyrosine is converted into DOPA via tyrosine
hydroxylase, which is the rate-limiting step in the synthesis of all the catecholamines. DOPA is then
converted to dopamine by DOPA decarboxylase. Dopamine is packaged into small synaptic vesicles
by the vesicular monoamine transporter (VMAT).
Unlike glutamate or GABA, dopamine-producing neurons are not widely abundant in the brain.
Instead, there are generally only a few patches of neurons that produce dopamine, most of which are
found in the midbrain. Two areas include the ventral tegmental area and the substantia nigra.
NEUROTRANSMITTERS: CATECHOLAMINES (DOPAMINE, NOREPINEPHRINE, EPINEPHRINE) | 197
Figure 18.1. Dopamine is synthesized in a two-step process. Tyrosine is converted into DOPA by tyrosine
hydroxylase, the rate-limiting step in the pathway. Then dopamine is synthesized from DOPA by DOPA
decarboxylase. Dopamine is then packaged into vesicles by vesicular monoamine transporter. ‘Dopamine
Synthesis’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-
Dopamine Receptors
Dopamine binds to metabotropic receptors on postsynaptic cells. There are two classes of dopamine
metabotropic receptors: D1-like receptors and D2-like receptors. D1-like receptors are typically
associated with Gαs and cause activation of the cAMP/adenylyl cyclase second messenger system and
the generation of EPSPs in the postsynaptic cell.
D2-like receptors, however, are typically associated with Gαi that inhibits the cAMP/adenylyl
cyclase second messenger system and generate IPSPs in the postsynaptic cell.
Figure 18.2.
Dopamine
Receptors.
Dopamine
(shown by the
‘D’) binds to
metabotropic
receptors. The
D1-like class of
receptors couples
to the Gαs
subunit and
activates
adenylyl cyclase,
which initiates
downstream
cellular effects. B)
The D2-like class
of dopamine
receptors couples
to the Gαi
subunit and
inhibits adenylyl
cyclase, which
prevents
downstream
cellular effects.
Termination of Dopamine Signaling

Dopamine action is terminated by reuptake into the presynaptic terminal via the dopamine
transporter (DAT). Once inside the cell, dopamine is either degraded via the actions of either
monoamine oxidase (MAO) or catechol-O-methyltransferase (COMT), or it is repackaged into
vesicles.
Figure 18.3. Dopamine action is terminated by reuptake into the presynaptic terminal via DAT.
Dopamine is then either degraded by MAO or COMT or repackaged into synaptic vesicles.
‘Dopamine Degradation’ by Casey Henley is licensed under a Creative Commons Attribution
Norepinephrine
Norepinephrine-producing cells are localized in the pons of the brain stem, a structure called the locus
coeruleus. The locus coeruleus is very small, but these neurons send projections widely throughout
the brain. Outside of the brain, we think of norepinephrine as being responsible for triggering the
sympathetic nervous system response of the body, the “fight-or-flight” reaction that prepares the body
for physical activity in times of fear or acute stress.
Synthesis
In neurons that release norepinephrine, which is another catecholamine transmitter, once dopamine is
packaged into small synaptic vesicles, a membrane-bound enzyme called dopamine beta-hydroxylase
converts dopamine into norepinephrine. Therefore, unlike the other small molecule
neurotransmitters, norepinephrine is synthesized within the vesicles, not in the cytoplasm. Like
dopamine, the rate-limiting step of this synthesis pathway is the activity of tyrosine hydroxylase.
Figure 18.4. Norepinephrine is synthesized from dopamine by dopamine beta-hydroxylase after

packaging into vesicles. ‘Norepinephrine Synthesis’ by Casey Henley is licensed under a Creative
Norepinephrine Receptors
Norepinephrine binds to metabotropic adrenergic receptors (α1, α2, and β). The binding of
norepinephrine to its receptor activates second messenger signaling cascades that will cause either
EPSPs or IPSPs, depending on the receptor subtype.
Figure 18.5. The norepinephrine beta-adrenergic receptor couples to the Gαs subunit and activates
adenylyl cyclase, which initiates downstream cellular effects. B) The norepinephrine alpha 2-adrenergic
receptor couples to the Gαi subunit and inhibits adenylyl cyclase, which prevents downstream cellular
effects. C) The norepinephrine alpha 1-adrenergic receptor couples to the Gαq subunit and activates
phospholipase C, which initiates downstream cellular effects. ‘Alpha Subunit Effects’ by Casey Henley is
Termination of Norepinephrine Signaling

Termination of norepinephrine signaling is similar to the termination of dopamine signaling.
Reuptake into the presynaptic terminal occurs via the norepinephrine transporter (NET), and
then the transmitter is either degraded within the cell by MAO or COMT or repackaged into
synaptic vesicles.
Figure 18.6. Norepinephrine action is terminated by reuptake into the presynaptic terminal via NET.
Norepinephrine is then either degraded by MAO or COMT or repackaged into synaptic vesicles.
‘Norepinephrine Degradation’ by Casey Henley is licensed under a Creative Commons Attribution
Epinephrine
Epinephrine, also called adrenaline, is a catecholamine, but it is often considered a hormone instead of
a neurotransmitter. Epinephrine is primarily released by the adrenal medulla into the circulation; it is
used as a neurotransmitter in only a small number of neurons.
Synthesis
Epinephrine is synthesized from norepinephrine in the cytoplasm by the enzyme
phenylethanolamine-N-methyltransferase, so epinephrine synthesis requires norepinephrine to
exit the vesicles where it was synthesized. After synthesis in the cytoplasm, epinephrine is repackaged
into small vesicles via the vesicular monoamine transporter.
Figure 18.7. Epinephrine is synthesized from norepinephrine by phenylethanolamine-N-

methyltransferase in the cytoplasm. Epinephrine is then packaged into vesicles by vesicular monoamine
transporter. ‘Epinephrine Synthesis’ by Casey Henley is licensed under a Creative Commons Attribution
Epinephrine Receptors
Epinephrine also binds to α and β adrenergic receptors (described above for norepinephrine) and
causes similar activity when bound to these receptors.
Termination of Epinephrine Signaling

Epinephrine, similarly to norepinephrine, also goes through reuptake into the presynaptic cell.
Further, it is also degraded within the cell by both MAO or COMT or repackaged into synaptic
vesicles.
Key Takeaways
• The catecholamines are synthesized from the amino acid tyrosine

• The synthesis pathways overlap and share many enzymes and proteins
• The catecholamines bind to metabotropic receptors
• The catecholamines are inactivated via similar mechanisms: reuptake, MAO and
COMT
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• Dopamine Receptors © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
NC-SA (Attribution NonCommercial ShareAlike) license
206 | NEUROTRANSMITTERS: ATYPICAL NEUROTRANSMITTERS
19.
NEUROTRANSMITTERS: ATYPICAL
NEUROTRANSMITTERS
Although we generally think of

neurotransmitters as neurochemicals that
function as described above, there are a
Resources few atypical neurotransmitters that don’t
quite fit the mold of the other chemical
signals.
• Glossary Terms
• Key Takeaways Neuropeptides
• Test Yourself
Neuropeptides are a class of large signaling molecules that
some neurons synthesize. Neuropeptides are different
from the traditional neurotransmitters because of their
chemical size. Neuropeptides are a short string of amino acids and are known to have a wide range of
effects from emotions to pain perception. Unlike small molecule neurotransmitters, neuropeptides are
synthesized in the cell body and transported to the axon terminal.
Synthesis and Storage

Like other proteins, neuropeptides are synthesized from mRNA into peptide chains made from amino
acids. In most cases, a larger precursor molecule called the prepropeptide is translated into the original
amino acid sequence in the rough endoplasmic reticulum. The prepropeptide is processed further to
the propeptide stage. The remaining processing and packaging of the final neuropeptide into a vesicle
occurs in the Golgi apparatus.
NEUROTRANSMITTERS: ATYPICAL NEUROTRANSMITTERS | 207
Monoamines like dopamine, norepinephrine, or serotonin have a molecular weight around

150-200, while one of the smaller neuropeptides, enkephalin, has a molecular weight of 570. One of
the largest neuropeptides, dynorphin, has a molecular weight greater than 2,000. Because of their large
size, neuropeptides have to be packaged in dense core vesicles very close to the site of production near
the nucleus, rather than in clear vesicles right at the terminal. These large vesicles must then move from
the soma to the terminal.
Figure 19.1. Neuropeptide synthesis occurs in the cell body. Each neuropeptide is encoded by a
gene on the DNA located in the nucleus. mRNA is translated into an amino acid sequence for a
precursor molecule called a prepropeptide in the rough endoplasmic reticulum. Further processing
and packaging of the neuropeptide into vesicles occurs in the Golgi apparatus. ‘Neuropeptide
Synthesis’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Axonal Transport of Neuropeptides

The packaged peptides need to be transported to the presynaptic terminals to be released into the
synaptic cleft. Organelles, vesicles, and proteins can be moved from the cell body to the terminal via
anterograde transport or from the terminal to the cell body via retrograde transport. Anterograde
transport can be either fast or slow.
The packaged neuropeptides are transported to the synaptic terminals via fast anterograde axonal
transport mechanisms.
Figure 19.2. Cellular components need to be able to move throughout the cell to have proper
functioning. Anterograde transport moves components from the cell body toward the terminal.
Retrograde transport moves components from the terminal toward the cell body. ’Axonal Transport’
by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-Alike (CC
Neuropeptide Receptors
Neuropeptides such as enkephalin and dynorphin are agonists at a class of receptors called the opioid
receptors. These opioid receptors fall into four main types. The three classical opioid receptors are
named using Greek letters: δ (delta), μ (mu), and κ (kappa); the fourth class is the nociceptin receptor.
All of these receptors are inhibitory metabotropic receptors which signal using the Gαi protein. These
receptors are expressed in several brain areas, but expression is particularly heavy in the periaqueductal
gray, a midbrain area that functions to inhibit the sensation of pain. Drugs that activate the opioid
receptors, like morphine, oxycontin, or fentanyl, are the most effective clinical treatments that we know
of for acute pain. Unfortunately, these same drugs also represent a tremendous health risk, as opioid
drugs can be lethal in overdose and have a high risk of substance use disorder.
Endocannabinoids
Endocannabinoids are endogenous lipid neurotransmitters that are a bit unusual compared to the
other neurotransmitters that we have covered. First, they signal in a retrograde manner. This means
that the endocannabinoids neurotransmitters are released by the postsynaptic cell and bind to
receptors on the presynaptic cell. Additionally, endocannabinoids are not packaged into vesicles and
released by fusion with the cell membrane.
Instead, when the postsynaptic cell has an action potential, it causes voltage-gated calcium
channels in the postsynaptic cell to open, and for calcium ions to rush into the postsynaptic cell.
The local increase in calcium concentration within the postsynaptic cell triggers the cell to synthesize
endocannabinoids. Again, these neurotransmitters are not stored in vesicles like the other
neurotransmitters that have been discussed. Instead, these lipid neurotransmitters are made on
demand, or synthesized ‘de novo’. As lipids, the endocannabinoids are membrane permeable and can
diffuse out of the cell through the cell membrane. The two most well-characterized eCBs in humans
are called 2-Arachidonoylglycerol (2-AG) and Anandamide (ANA).
The endocannabinoids bind to one of two receptors, CB1 or CB2. Both receptor types are
inhibitory metabotropic receptors that are located on the presynaptic cell and coupled to Gαi.
Generally, CB1 receptors are found in the nervous system, while the CB2 receptors are found elsewhere
in the body, such as in the immune system. Activity of the metabotropic CB1 receptors causes
presynaptic calcium channels to close, reducing the concentration of calcium in the presynaptic cell,
and thus decreasing the amount of neurotransmitter released by the presynaptic cell.
The endocannabinoid system is widely used by various systems in the body. It is estimated that
endocannabinoid receptors are the most abundant GPCRs in the whole body. These substances were
named because they are endogenous chemicals that are functionally similar to compounds found in
plants of the genus Cannabis. One reason cannabis is used is because of its ability to interact with our
endocannabinoid receptors.
Figure 19.3. Endocannabinoid Signaling. When the postsynaptic terminal is stimulated, it causes the
voltage-gated calcium channels to open in the postsynaptic cell. Calcium rushes into the
postsynaptic cell and causes intracellular enzyme (shown in green) to produce lipid
endocannabinoids. The endocannabinoids signal in a retrograde fashion to the presynaptic cell,
where they bind to metabotropic CB1 receptors. Activation of the CB1 receptors causes closing of
presynaptic voltage-gated calcium channels, and decreased release of neurotransmitters from the
presynaptic cell. ‘Endocannabinoid Signaling’ by Valerie Hedges is licensed under a Creative
Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Nitric Oxide
The nervous system is capable of signaling via the gas nitric oxide (NO). This gas transmitter is not
stored in vesicles, but is synthesized as it is needed. NO is formed when the amino acid arginine is
degraded by the enzyme NO synthase (NOS). Because NO is a gas, it easily permeates across cell
membranes. Therefore, the receptors for NO do not need to be transmembrane proteins expressed on
the cell surface. Instead, the receptor for NO is an intracellular receptor called soluble guanylate cyclase
(sGC). SGC works through a signaling pathway that is different from other metabotropic receptors
so far described. SGC is linked with the signaling molecule cyclic GMP (cGMP), which activates
protein kinase G (PKG). PKG can either be excitatory or inhibitory, depending on the intracellular
components.
Key Takeaways
• Neuropeptides are proteins that are synthesized in the cell body and are transported
in vesicles to the axon terminal
• Endocannabinoids are lipid neurotransmitters that use retrograde signaling. They are
synthesized and released by the postsynaptic cell and bind to metabotropic CB1
receptors on the presynaptic cell to alter neurotransmitter release.
• Nitric oxide is a gas neurotransmitter that is not stored in vesicles but instead is made
on demand. As a gas, nitric oxide can easily cross the phospholipid bilayer and bind to
intracellular receptors.
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DRUG AND TOXIN EFFECTS | 213
20.
DRUG AND TOXIN EFFECTS
Drugs and toxins can alter neuron functioning in a range

of ways, from activation to inhibition and all levels of
modulation. Although many drugs exist that alter Resources
molecular process typical of many cells, this lesson will
focus on neuron-specific targets.
• Glossary terms
Synaptic Effects • Key Takeaways

• Test Yourself
As we have seen, the synapse is an incredibly complex

structure, and for small molecule neurotransmitters, the
entire “lifecycle” of the transmitter occurs in this space:
synthesis, packaging, release, action, and termination. This means there are numerous targets upon
which drugs and toxins can act to alter synaptic communication.
Drug Effects on Neurotransmitter Release

Drugs can alter neurotransmitter synthesis pathways, either increasing or decreasing the amount of
neurotransmitter made in the terminal, affecting how much transmitter is released. An example of
this is administration of L-DOPA, a dopamine precursor molecule in the dopamine synthesis pathway
that results in increased dopamine production; it is used as a treatment for Parkinson’s Disease.
Neurotransmitter packaging is another site of possible drug action. Reserpine, which has been used
to treat high blood pressure, blocks the transport of the monoamine transmitters into vesicles by
inhibiting the vesicular monoamine transporter (VMAT). This decreases the amount of
neurotransmitter stored and the amount of neurotransmitter released in response to an action
potential.
214 | DRUG AND TOXIN EFFECTS
Figure 20.1. Drugs and toxins can alter neurotransmitter synthesis and packaging into synaptic
vesicles. L-DOPA increases the synthesis of dopamine in the terminal. Reserpine prevents packaging
of the biogenic amines, resulting in low concentrations of transmitter stored in synaptic vesicles.
‘Drug Effects on Neurotransmitter Release’ by Casey Henley is licensed under a Creative Commons
Drug Effects on the Postsynaptic Membrane

The neurotransmitter receptors are another critical location for drug and toxin action. Agonists
mimic neurotransmitter effects, whereas antagonists block neurotransmitter effects. Muscimol, a
component of some mushrooms, is an agonist for the ionotropic GABA receptor. Bicuculine, a
component of some plants, is an antagonist to this receptor and blocks the action of GABA.
Additionally, many chemicals are able to modulate receptors in either a positive or negative fashion.
Alcohol binds to the GABA receptor and increases the time the receptor is open when GABA binds.
Figure 20.2. Drugs and toxins can alter neurotransmitter receptors on the postsynaptic neuron. A
GABA agonist, muscimol, would replicate the actions of GABA and cause an IPSP. A GABA
antagonist, bicuculine, would prevent GABA actions resulting in no IPSP. Modulators such as
alcohol, alter how the receptor works, so when GABA binds the response is a stronger IPSP than
when alcohol is not present. ‘Postsynaptic Drug Effects’ by Casey Henley is licensed under a
License.
Drug Effects on Neurotransmitter Clearance

Finally, neurotransmitter degradation and reuptake can also be altered by drugs and toxins.
Depending on the neurotransmitter, enzymes located in either the synapse or in the terminal are
responsible for degradation of the transmitter, and these enzyme can be blocked by drugs.
Organophosphates are found in many pesticides and prevent the action of acetylcholinesterase, the
enzyme that breaks down acetylcholine in the synapse. This inhibition increases acetylcholine action
on the postsynaptic neuron. Monoamine oxidase inhibitors (MAOIs) prevent monoamine oxidase
from degrading the biogenic amine neurotransmitters. MAOIs have been used as antidepressants
since they increase the amount of transmitter available. Additionally, drugs can prevent the reuptake
of neurotransmitters into the presynaptic terminal. Cocaine blocks the dopamine transporter, which
results in increased action of dopamine in the synapse.
Figure 20.3. Drugs and toxins can alter neurotransmitter degradation and reuptake into the
presynaptic terminal. Organophosphates prevent the degradation of acetylcholine in the synapse.
MAOIs prevent the degradation of monoamine transmitters in the terminal. Cocaine prevents
dopamine from being transported into the presynaptic terminal. All of these effects lead to
increased neurotransmitter action and availability. ‘Drug Effects on Neurotransmitter Clearance” by
Non-Synaptic Effects
Drugs and toxins can also affect neuron function by acting outside of the synapse. For example,
some chemicals change voltage-gated ion channel dynamics. Veratridine, a compound found in plants
from the lily family, prevents voltage-gated sodium channels from inactivating. Initially, this causes an
increase in neurotransmitter release, but it can quickly lead to excitotoxicity.
Key Takeaways
• There are many ways in which drugs and toxins can alter neuron function
• Effects can be excitatory, inhibitory, or modulatory
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NERVOUS SYSTEM ORGANIZATION | 219
PART IV
NERVOUS SYSTEM
ORGANIZATION
220 | NERVOUS SYSTEM ORGANIZATION
ANATOMICAL TERMINOLOGY | 221
21.
ANATOMICAL TERMINOLOGY
The nervous system is one of the most complex systems

that we know of. Parts of this system malfunction
frequently, and the results are a wide range of neurological Resources
disorders that affect humans, from injury to genetic
disorders. The gross anatomy of the nervous system is an
important foundation to the studies of other aspects of
• Glossary Terms
neuroscience. This chapter covers some of the major
• Key Takeaways
anatomical structures in the nervous system.
• Test Yourself
Divisions of the Nervous

System
Broadly speaking, the nervous system can be divided into two main categories: The central nervous
system (CNS) and the peripheral nervous system (PNS). Simply put, the CNS is the brain and spinal
cord, while the PNS is all the other nerve cells in the body in the periphery. The two systems are
not isolated from each other; information passes rapidly between the PNS and the CNS, and vice
versa. When a signal that originates in the PNS moves to the CNS, we sometimes say that the signal is
incoming or ascending, while the CNS to PNS direction is outgoing or descending. Information that
arrives into the CNS is also called an afferent signal, while information leaving the CNS is an efferent
signal. These two terms are frequently confused, but you can use the knowledge of other words that
start with “e” to remember that an “exit” or an “escape” is something that moves away for ‘efferent’,
and “arrive” for ‘afferent’. Alternatively, an efferent signal is something that has an effect on the outside
world, while an afferent signal affects the person.
222 | ANATOMICAL TERMINOLOGY
Figure 21.1. The

nervous system
is divided into
the central
nervous system,
which includes
the brain and
spinal cord, and
the peripheral
nervous system,
which includes
the cranial and
spinal nerves.
Information
traveling toward
the brain is called
afferent,
whereas
information
traveling from
the brain is called
efferent. ‘CNS
and PNS’ by
Casey Henley is
licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Anatomically, the CNS consists of two organs, the brain and the spinal cord. The brain is the main
organ where movement originates, where thoughts and plans develop, and where consciousness is
housed. The brain is what pushes us to act on our drives and desires, where language begins, and where
memories are stored. The intact adult brain weighs about 1.5 kg (3 pounds), which is barely 2% of total
body weight. Despite this relatively small size, it is extremely power hungry, and uses up about one-fifth
of the body’s total energy expenditure.
Anatomical Terms
When talking about parts of the brain, it is helpful to have a set of directional terms that can describe
the location of various anatomical structures unambiguously.
Familiarity with the terminology used to describe location and relationships within the nervous
system is critical as we move forward into examining brain systems.
Directional Terms
Directional terms are used to locate one structure, usually in relation to another structure. Some
terms, like dorsal or ventral, are relative to the axis of the central nervous system, so the direction these
terms define changes if used for brain regions versus other body regions. Other terms, like superior or
inferior, keep their meaning across the entire body.
• Anterior: In front of; toward the face

• Posterior: Behind; toward the back
• Superior: Above; toward the head
• Inferior: Below; toward the feet
• Medial: Toward the middle
• Lateral: Toward the edge
• Dorsal: Toward the top of the brain or the back of the spinal cord
• Ventral: Toward the bottom of the brain or the front of the spinal cord
• Rostral: Toward the front of the brain or the top of the spinal cord
• Caudal: Toward the back of the brain or the bottom of the spinal cord
Often anterior and rostral are used interchangeably and posterior and caudal are used interchangeably.
Neuroanatomists use all of these terms to describe the relationship of one structure to another.
For example, we’ll see in Chapter 22 that introduces the four lobes of the brain, the frontal lobe is
anterior or rostral to the parietal lobe, and the parietal lobe is dorsal to the temporal lobe. These
anatomical words can also be combined to subdivide complex brain regions. For example, a structure
called the thalamus has many small subsections, such as the dorsomedial nucleus or the ventropostero-
lateral nucleus. Naming structures with this anatomical language is useful in identifying where they
are located in a brain scan or autopsy, but these words tell us nothing about function.
Figure 21.2. Directional terms used to locate nervous system structures. The dorsal / ventral and
rostral / caudal pairs point in different directions depending on if they are referring to the axis of the
brain (orange arrows) or the axis of the spinal cord (blue arrows). The definitions of each term are
described in the text. ‘Anatomical Directions’ by Casey Henley is licensed under a Creative Commons
Visualizing the Brain: Anatomical Planes

As a three-dimensional structure, the brain can be sectioned, or cut, for visualization or analysis in
several ways. Here, we will describe the three main orientations, each at right angles to the others.
1. The frontal or coronal plane is a vertical plane in a medial to lateral direction, dividing objects
into front and back pieces.
2. The sagittal plane is also a vertical plane but in a rostral-caudal direction, meaning it divides
objects into right and left regions.
3. Finally, the horizontal plane divides objects into top and bottom regions.
Figure 21.3. Three anatomical planes are used to divide the nervous system to be able to view
internal regions and structures. The frontal or coronal plane is a vertical plane that runs parallel to
the eyes or ears and will divide the body into front and back regions. The sagittal plane is a vertical
plane that runs perpendicular to the eyes or ears and will divide the body into left and right regions.
The horizontal plane runs parallel to the ground and will divide the body into top and bottom
regions. ‘Anatomical Planes’ by Casey Henley is licensed under a Creative Commons Attribution
Gray Matter and White Matter

In a sliced section of the brain, you might notice that brain tissue has different colored areas. Some
brain tissue is pale and almost white, and these areas are described as white matter. Generally,
white matter represents pathways of communication. White matter regions are comprised of axons.
It appears white due to the myelin sheath on the axons. Other sections of brain tissue have a darker
pink/gray color, appropriately called gray matter. These areas are usually dense with cell bodies and
dendrites. Gray matter is the location of most synapses.
Figure 21.4. The central nervous system tissue can be divided into white and gray matter. White
matter is primarily myelinated axons. Gray matter is primarily neuronal cell bodies and dendrites. In
the brain, the surface of the cerebral cortex is a layer of gray matter. White matter can be found
below the gray matter layer and is the location of the axons traveling to and from the cortical cell
layer. Gray matter can also be found deep in the brain in subcortical regions that play critical roles in
behavior. ‘White and Gray Matter’ by Casey Henley is licensed under a Creative Commons
White Matter Tracts

The brain has two very similar halves, the left and right hemisphere. Oftentimes, in the neurotypical
individual, information passes between both hemispheres rapidly: what one hemisphere senses or
learns, so does the other hemisphere. It is the white matter tracts that allow for this transfer of
information. When a white matter pathway crosses from one side of the body to another, we call it a
decussation.
Commissures are white matter tracts that connect gray matter between the left and right
hemispheres of the brain. The main commissure that allows for the passage of information between
the two hemispheres is called the corpus callosum.
Association fibers are white matter tracts that connect different bits of gray matter within the same
hemisphere of the brain. These connections do not cross the midline.
Projection fibers are white matter tracts that connect the hemispheres of the brain with lower brain
structures or with the spinal cord. They “project” from the cortex to the lower brain structures or
spinal cord.
Figure 21.5.
Commissures
(red) connect
gray matter
between the left
and right
hemispheres.
Association
fibers (blue)
connect gray
matter within the
same hemisphere
of the brain.
Projection fibers
(green) connect
gray matter with
lower brain
structures or the
spinal cord.
‘White Matter
Connections’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Key Takeaways
• Anatomical terminology is critical for determining neurological landmarks and

discussing the anatomy of the nervous system
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• White Matter Connections © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

BRAIN STRUCTURE DIFFERENTIATION | 229
22.
BRAIN STRUCTURE DIFFERENTIATION
In the following chapters, some of the many structures

that make up the brain will be introduced. To begin this
discussion, we will identify some of the basic development
of the nervous system to understand how different areas Resources
of the brain are organized.
Very early in development, an embryo looks like a flat
disc with 3 different layers of cells.
• Glossary Terms
1. Endoderm: will eventually develop into the viscera • Key Takeaways
(organs of the body) • Test Yourself
2. Mesoderm: will eventually develop into the bones
and muscles
3. Ectoderm: will eventually develop in the nervous
system and the skin
230 | BRAIN STRUCTURE DIFFERENTIATION
Figure 22.1.
Developmental
layers of the
embryo. Early in
development, the
embryo looks like
a flat disc made
up of three
different layers
(left image). The
ectoderm layer is
what will
develop into the
nervous system.
A cross section of
the embryo
(right image)
reveals the
endoderm,
mesoderm, and
ectoderm layers.
‘Developmental
layers of the
embryo’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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The Neural Tube

Let’s focus on the ectoderm that will develop into the nervous system. The ectoderm layer will fold
together in a process called neurulation to form a neural tube. Neurulation occurs within the first
month following conception in humans.
During neurulation, the middle of the neural plate folds first, followed by the anterior and posterior
ends. The central nervous system will develop from the walls of the neural tube and the hollow lumen
of the tube will become the cerebral ventricles and spinal canal within the central nervous system.
Figure 22.2.
Neurulation.
During the
process of
neurulation, the
neural plate
pinches together
at the neural fold
in the middle,
and then closes
at the anterior
and posterior
ends to form a
neural tube. The
structures of the
central nervous
system will form
from the walls of
the neural tube
and the lumen of
tube will become
the ventricles of
the brain and
central canal of
the spinal cord.
‘Neurulation’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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Neural Tube Defects
Early in pregnancy, there can be problems with the process of neurulation and the
formation and closure of the neural tube. Collectively, these are referred to as neural tube
defects that include defects that occur within the brain, spine, or spinal cord. One of the
more common neural tube defects is spina bifida. In spina bifida, the neural tube fails to
close completely, and as a result the backbone that typically protects the spinal cord also
does not form appropriately. This event can occur anywhere along the spine, and will
typically result in nerve damage at that particular site that will potentially lead to physical
and / or intellectual disability ranging in severity with the degree of damage.
Anencephaly is a neural tube defect that is caused by the failure of the anterior portion
of the neural tube to close. Anencephaly is a serious birth defect that typically prevents the
development of the anterior portion of the brain and associated skull.
Unfortunately, many neural tube defects occur very early in pregnancy, such that many
women do not yet know that they are pregnant. As a result, daily folic acid supplements or
a diet that includes foods rich in folate is recommended to all women of reproductive age to
help prevent neural tube defects in the event of a pregnancy.
Figure 22.3.
Neural Tube
Defects. Spina
bifida (left) occurs
when the
posterior neural
tube fails to close.
Anencephaly
(right) occurs
when the
anterior neural
tube fails to close,
causing
decreased
development of
the brain and
skull. ‘Neural
Tube Defects’ by
Valerie Hedges is
licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Three Vesicle Stage of Development

Early in development, the anterior portion of the neural tube has three distinct vesicles, which will
each develop into different structures. These vesicles, from most anterior to most posterior, are the
prosencephalon (forebrain), the mesencephalon (midbrain), and the rhombencephalon
(hindbrain).
Through development, the walls of these vesicles will differentiate into adult brain structures.
Differentiation is the process by which structures become more complex and functionally specialized
during development. The names of these early vesicles can be used to describe either the stages through
development, or a grouping of structures that eventually form in adulthood. In the following chapters,
we will learn more about some of these anatomical structures.
Figure 22.4.
Three vesicle
stage of
embryonic
development.
Early in
development the
anterior portion
of the neural
tube can be
divided into three
vesicles: the
prosencephalon,
the
mesencephalon,
and the
rhombencephalo
n. ‘Three vesicle
stage’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Prosencephalon (Forebrain)
The prosencephalon, or the forebrain, is most anterior in the neural tube and eventually develops
into the “higher order” brain regions, including the cerebral cortex. Most of the time, when you see an
image of an intact brain from the side or the top, the structures that are visible to you are the forebrain
structures. The prosencephalon is made up of the telencephalon and the diencephalon.
As the prosencephalon continues to develop, additional structures will form that are associated with
the cerebral hemispheres. For instance, the optic vesicles that bud off the surface of the prosencephalon
will differentiate into the retinas of the eyes—thus, the retina is made up of neural tissue. The olfactory
bulbs will differentiate from the ventral surface of the cerebral hemispheres.
Note that as the walls of the tube develop into these structures, the lumen of the tube remains
and will become the fluid-filled ventricles of the brain. The lateral ventricles will form from the tube
within the cerebral hemispheres and the third ventricle will be found ventrally surrounded by the
diencephalon.
Structures of the Telencephalon: Cerebral Cortex and

the Basal Ganglia
The cerebral cortex makes up the outermost layer of the brain. The word “cortex” comes from the
word meaning “bark”, the outer layer of a tree. The cerebral cortex is the most evolved structure of the
human brain and is responsible for higher order thinking. Here, the brain processes behaviors such as
attention, memory, and language.
The basal ganglia are made up of a series of brain structures (including the caudate and putamen,
globus pallidus, substantia nigra, and subthalamic nucleus) that are used for such behaviors as motor
and habit learning, emotional processing, and action selection.
Figure 22.5. The basal ganglia are subcortical structures located at the base of the forebrain. They
are comprised of the caudate and putamen, which both make up the striatum, as well as the globus
pallidus, substantia nigra, and subthalamic nucleus. ‘Basal Ganglia’ by Casey Henley is licensed
Structures of the Diencephalon: Thalamus and

Hypothalamus
The thalamus is often referred to as a “sensory relay station” in the brain, since almost every sensory
modality (sight, taste, touch, and hearing) passes information through the thalamus before being
directed to the appropriate area of the cortex.
The hypothalamus is also within the diencephalon. This structure serves as an autonomic control
center to alter visceral function and a communication route to the body’s endocrine system through
control of anterior pituitary hormones and production of posterior pituitary hormones. Neural signals
originating in the hypothalamus have the capability to influence the chemistry and function of the
entire body. The hypothalamus also has nuclei that function in emotional responses, and regulate body
temperature, food intake, water balance, and sleep.
Figure 22.6. The

pituitary, shown
in green in a
mid-sagittal
section, lies
inferior to the
hypothalamus,
shown in blue.
‘Hypothalamus
and Pituitary’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Mesencephalon
Moving more caudally, the mesencephalon (or the midbrain) differentiates into the tectum and the
tegmentum.
Tectum
The tectum (which means “roof”) is the dorsal portion of the midbrain and it has two major
structures: the superior colliculus and inferior colliculus. The superior colliculus is important in
reflexive eye movements that allow you to quickly orient to something changing in your environment.
The inferior colliculus relays auditory information to the thalamus for auditory function.
Tegmentum
The tegmentum (which means “floor”) is the ventral portion of the midbrain. There are many
structures in the tegmentum and they can perform a wide variety of functions. For example, the
periaqueductal gray allows us to respond to painful stimuli, the red nucleus and substantia nigra
coordinate complex movements, and the ventral tegmental area is important for the processing of
reward and motivation.
Figure 22.7. A
midsagittal
section of the
brain. The
midbrain (tectum
and tegmentum)
are located
anterior to the
pons. ‘Internal
Brain Regions’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Rhombencephalon
The most posterior/caudal portion of the neural tube is the rhombencephalon (or hindbrain).
Evolutionarily speaking, the rhombencephalon represents the oldest part of the central nervous system.
These structures likely evolved some 570 million years ago.
Rostral Hindbrain
The more rostral portion of the hindbrain differentiates into two structures: the cerebellum and
the pons. The cerebellum, or “little brain”, is best known as a structure that enables motor control
functions, such as balance, coordination, posture, and learning physical actions. The cerebellum helps
us recognize and predict sequences of events during motor learning. More recently, the cerebellum
has also been recognized to play a role in non-motor functions like learning. The pons (which means
“bridge”) is an important structure that relays impulses between the motor cortex and the cerebellum.
It has a critical function to help us perform involuntary functions like breathing.
Caudal Hindbrain
The caudal portion of the hindbrain differentiates into the medulla. The medulla is found at the
far posterior end of these three early developmental vesicles. The medulla contains many clumps of
neurons that are responsible for functions that an organism carries out unconsciously. It contains a
cardiovascular center that is especially important in maintaining and changing blood pressure and
heart rate, and also has connections to the pons to help regulate breathing. The medulla contains
areas that can detect toxins in the blood that come from dietary sources, triggering vomiting. Other
behaviors like hiccupping, swallowing, coughing, and sneezing are also controlled by the medulla.
Moving beyond this anterior portion of the neural tube that contains the prosencephalon,
mesencephalon, and rhombencephalon, the neural tube continues down the length of the embryo.
The remainder of the neural tube will differentiate into the spinal cord, with the lumen of the tube
becoming the central canal of the spinal cord. The spinal cord is important in relaying information to
and from the body.
Figure 22.8.
Structures of the
rhombencephalo
n. The rostral
portion of the
rhombencephalo
n develops into
the cerebellum
and pons. The
caudal portion of
the of the
rhombencephalo
n develops into
the medulla. The
spinal cord is
connected to the
posterior end of
the medulla and
continues down
the length of the
spine. ‘Structures
of the
rhombencephalo
n’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Key Takeaways
• The ectoderm layer of the embryo develops into the nervous system
• Neurulation is the process that forms the neural tube and failure for portions of the
neural tube to close can cause neural tube defects
• The prosencephalon, mesencephalon, and rhombencephalon are the three vesicles at
the anterior end of the neural tube. They will develop into structures in the adult
brain.
Test Yourself!
Attributions
Media Attributions
• Three layers of the embryo © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Neurulation © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Neural Tube Defects © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• 3 vesicle stage of development © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Sagittal section of brain structures © Casey Henley is licensed under a CC BY-NC-SA
(Attribution NonCommercial ShareAlike) license
• Structures of the rhombencephalon © Valerie Hedges is licensed under a CC BY-NC-SA
BRAIN ANATOMY | 243
23.
BRAIN ANATOMY
The brain is comprised of the cerebrum, cerebellum, and

brainstem. The cerebrum is the most prominent region of
the brain. It is divided into left and right hemispheres. The Resources
hemispheres have many of the same functions. For
example, each perceives touch on one side of the body, but
some functions demonstrate laterality, meaning they are
• Glossary Terms
primarily controlled on one side of the brain.
• Key Takeaways
Most of what we think of when we imagine the brain is
• Test Yourself
the cerebral cortex in humans. The cortex has many folds
to increase the surface area of the brain. The bumps or
raised ridges on the outer surface are called gyri (singular
gyrus) and the grooved indentations are called sulci (singular sulcus). A large, deep sulcus is sometimes
also called a fissure.
Although each gyrus and sulcus has a name that either identifies its function or location, there are
only three sulci that we will introduce here to help orient us around the neuroanatomical features
of the cortex. The longitudinal fissure is the most obvious fissure in the brain. It divides the two
hemispheres, running along the anterior–posterior axis, visible from a dorsal view of the brain. If you
were to cut along the longitudinal fissure completely, you would get two symmetrical portions of brain:
the left and right hemispheres.
The central sulcus is a large fissure that starts at the dorsal part of the brain at about the halfway
point on the anterior–posterior axis. In a sagittal view, the central sulcus runs ventrally about half the
length of the brain. The other groove worth noting is the lateral fissure. This one runs roughly along
the anterior-to-posterior direction, and curves gently dorsally. Again, in a sagittal view, it is roughly
seen in the middle third of the brain in the anterior–posterior axis.
244 | BRAIN ANATOMY
Figure 23.1 An external side view of the parts of the brain. The cerebrum, the largest part of the
brain, is organized into folds called gyri and grooves called sulci. The cerebellum sits behind
(posterior) and below (inferior) the cerebrum. The brainstem connects the brain with the spinal cord
and exits from the ventral side of the brain. ‘External Brain Regions’ by Casey Henley is licensed
Anatomical Lobes of the Cortex

The cortex is roughly divided into four major lobes, which are named after the bones of the skull that
surround each section of brain. The lobes are paired, meaning that the whole brain contains two of
each, a left and a right. In general, the structures are roughly symmetrical.
Frontal Lobes
The frontal lobes are the most rostral, located in the front of the brain. The posterior border of the
frontal lobe is the central sulcus. Among mammals, it is the largest of the four lobes. The frontal
lobes are responsible for higher level executive functions like attention, critical thinking, and impulse
control. The frontal lobes allows us to do mental math, to hold a string of letters in our head to be
BRAIN ANATOMY | 245
repeated backward, and to suppress socially unacceptable actions. Our personality is influenced by the
frontal lobe. An injury to these brain structures can result in a radical change in a person’s behavior.
They are the last brain region to fully develop, not completing development until individuals reach
their mid 20s. The frontal lobes are also the location of the primary motor cortex, the region of
the brain responsible for planning and executing movement. Specifically, the primary motor cortex is
located in the precentral gyrus, which is directly anterior to the central sulcus.
Figure 23.2. The frontal lobe is located in the front of the brain. It includes the precentral gyrus, the
location of the primary motor cortex. ‘Frontal Lobe’ by Casey Henley is licensed under a Creative
View the frontal lobe using the BrainFacts.org 3D Brain

246 | BRAIN ANATOMY
Phineas Gage
Phineas Gage, who was mentioned back in Chapter 1, is one of the most famous case
studies in neuroscience.
The mid-1800s saw an expansion of industry in the United States. The most reliable and
quickest way to move goods and people was with the railways that were starting to zig-
zag across the growing country. The factor slowing down railway expansion was terrain:
land had to be relatively flat for the tracks to be laid down. Terraforming mountains was a
dangerous ordeal, and in the years before TNT (a relatively safe explosive) was developed,
work accidents were a risk that these demolition workers faced.
Phineas Gage was one of those workers. In the green mountain hills of Vermont, Gage was
putting explosive powder into a crack in a mountain to clear land for a new railway. As
Gage packed the explosive using a three-foot-long metal rod, a spark accidentally ignited
the blast prematurely, causing the tamping iron to rocket cleanly through Gage’s skull.
Miraculously, Gage survived the blast. Within a month, he had made an almost complete
recovery. Gage was talking excitedly with his doctors, he was eating voraciously, and even
reported experiencing no pain. While the doctors noticed that his entire frontal lobe had
been destroyed, it was his friends who noticed the dramatic change in his personality:
whereas he was once a friendly man, adored and respected by his coworkers, the new
Gage was irreverent, generally unlikable, and prone to using profanity at the most
inappropriate times. The pre-injury Gage was a shrewd businessman who followed
through with his plans, but Gage now was unreliable and at times, acted in a more like an
animal. Because of the injuries to his frontal lobe, his friends described him as being “no
longer Gage”.
BRAIN ANATOMY | 247
Figure 23.3. Image of Phineas Gage and his site of injury. The figure on the left is an image of Phineas
Gage holding the iron rod that caused his injury. The image on the right shows a Magnetic Resonance
Image rendering of the location of the rod in the injury. The rod entered below the left eye and
damaged much of the left frontal cortex.
Parietal Lobes
The central sulcus lies caudal to the frontal lobe and divides the frontal lobes from the parietal
lobes. The primary somatosensory cortex is located in the postcentral gyrus of the parietal lobe
and is responsible for the perception of touch and pain. With our skin, we are able to detect light
touch, temperature, pain, vibration, and many other modalities. This ability to sense different tactile
248 | BRAIN ANATOMY
properties of things in the world around us with our body is one of the major functions of the parietal
lobe. Another closely related sense, proprioception (the ability to identify where parts of your body are
located), is also processed by neurons of the parietal lobe. The parietal lobes also perform higher-level
visual processing.
Figure 23.4. The parietal lobe is located on the top of the brain. It includes the postcentral gyrus, the
location of the primary somatosensory cortex. The central sulcus divides the parietal lobe from the
frontal lobe. ‘Parietal Lobe’ by Casey Henley is licensed under a Creative Commons Attribution
View the parietal lobe using the BrainFacts.org 3D Brain
Temporal Lobes
The temporal lobes are located on the side of the brain, separated from the frontal and parietal lobes
by the lateral fissure. Like the parietal lobe, the temporal lobe plays a role in sensory processing,
specifically with hearing, smell, taste, and higher-level visual processing. The auditory system allows
our brain to interpret sound waves. The ability to remember important facts depends on memory-
related processes. These functions are carried out in part by a brain structure called the hippocampus,
BRAIN ANATOMY | 249
which is buried medially and ventrally in the temporal lobe. The temporal lobe also houses some
structures that are important for language.
Figure 23.5. The temporal lobe is located on the side of the brain. The lateral fissure divides the
temporal lobe from the frontal and parietal lobes. ‘Temporal Lobe’ by Casey Henley is licensed under
License.
View the temporal lobe using the BrainFacts.org 3D Brain
Occipital Lobes
The occipital lobes, the most caudal lobes, are located in the back of the brain. Anatomically, there is
not an obvious border that separates the occipital lobe from adjacent areas of the cortex. The occipital
lobe is the smallest of the four lobes. The main function of the occipital lobe is for processing of visual
stimuli. Our eyes are capable of capturing light and converting that light into signals. The primary
visual cortex of the occipital lobe, also called V1, interprets those signals into a representation of the
visual world. Other vision-related stimuli, such as objects in motion, object orientation, and color are
also processed by neurons in the occipital lobe.
250 | BRAIN ANATOMY
Figure 23.6. The occipital lobe is located in the back of the brain. ‘Occipital Lobe’ by Casey Henley is
View the occipital lobe using the BrainFacts.org 3D Brain
Non-Cerebral Components
The cerebellum lies inferior to the occipital lobes. The cerebellum is divided into two hemispheres
like the cerebral cortex. The cerebellum is best known for its role in regulation and control of
movement, but it is also involved in cognitive functions like emotions.
The brainstem is located between the cerebrum and the spinal cord. It is important for regulating
critical functions like heart rate, breathing, and sleep. It is also the location of most of the cranial nerves.
The spinal cord, which is part of the central nervous system but not part of the brain, is
responsible for receiving sensory information from the body and sending motor information to the
body. Involuntary motor reflexes are also a function of the spinal cord, indicating that the spinal cord
can process information independently from the brain.
BRAIN ANATOMY | 251
Figure 23.7. The cerebellum, brainstem, and spinal cord are located below the brain. ‘Hindbrain’ by
View the brainstem using the BrainFacts.org 3D Brain

View the cerebellum using the BrainFacts.org 3D Brain
Dorsal View
Viewing the brain from above shows the bilateral symmetry of the left and right cerebral hemispheres,
which are separated by the longitudinal fissure. The frontal, parietal, and occipital lobes can be seen.
Similar to the lateral view, the central sulcus divides the frontal lobe from the parietal lobe. The
precentral gyrus—which is the location of the primary motor cortex—sits rostral to the central sulcus,
whereas the postcentral gyrus—which is the location of the primary somatosensory cortex—lies
caudal to the central sulcus.
252 | BRAIN ANATOMY
Figure 23.8. The dorsal view of the brain. The left and right cerebral hemispheres are separated by
the longitudinal fissure. Three of the four lobes (the frontal, parietal, and occipital) can be seen in
this view. ‘Dorsal Surface of Brain’ by Casey Henley is licensed under a Creative Commons
Ventral View
Underneath the brain, the frontal and temporal lobes are visible, as is the cerebellum. Like the dorsal
view, the longitudinal fissure divides the cerebrum into right and left hemispheres. The pons and
medulla (components of the brain stem) connect the cerebrum to the spinal cord.
BRAIN ANATOMY | 253
Fig 23.9. Ventral Surface of the Brain. The frontal lobe, temporal lobe, cerebellum, pons, medulla,
spinal cord, and longitudinal fissure can be seen when viewing the bottom of the brain. “Ventral
Surface of the Brain” by Casey Henley is licensed under a Creative Commons Attribution
Cranial nerves are also visible on the ventral surface of the brain. The olfactory tract leads out to the
olfactory bulb, which connects to the olfactory nerve. The optic tract crosses the midline at the
optic chiasm, and then the optic nerve projects to the retina. Other cranial nerves enter or leave the
brain at the level of the brainstem. The hypothalamus is located caudal to the pons and the
mammillary bodies project out from the hypothalamus.
254 | BRAIN ANATOMY
Figure 23.10. Cranial nerves, optic chiasm, and olfactory tract are visible on the bottom of the brain.
In the center, the hypothalamus and mammillary bodies can also be seen. “Ventral Surface Cranial
Nerves” by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
There are twelve pairs of cranial nerves that are outlined in the table below.
BRAIN ANATOMY | 255
Table 23.1. A table that defines each of the twelve cranial nerves by identifying their name, the type of
nerve, and function.
Type of
Cranial Nerve Function
Nerve
CN I Olfactory nerve Sensory Sense of smell
CN II Optic nerve Sensory Sense of vision
CN III Oculomotor Control of extraocular muscles that allow movement of eyeballs;

Motor
nerve constriction of pupils; changing of lens shape
Control of the superior oblique muscle of the eye that moves the
CN IV Trochlear nerve Motor
eyeball down and lateral
Sensory + Tactile and pain sensory information from the face and mouth;
CN V Trigeminal nerve
Motor Control of muscles used in chewing
Control of the lateral rectus muscle of the eye that moves the
CN VI Abducens nerve Motor
eyeball outward laterally
Sensory + Control of the muscles that allow for facial expressions; Taste
CN VII Facial nerve
Motor sensation on the anterior two thirds of the tongue
CN VIII Detection of sound information and head positional (vestibular)

Sensory
Vestibulocochlear nerve information
Detection of somatic sensory in the middle ear and posterior third

CN IX Sensory + of the tongue; Taste sensation on the posterior third of the
Glossopharyngeal nerve Motor tongue; Controls the stylopharyngeal muscle that allows
swallowing
Sensory + Control of the internal organs by autonomic nervous system using

CN X Vagus nerve
Motor parasympathetic activity
Control of the sternocleidomastoid and trapezius muscles of the

CN XI Accessory nerve Motor
neck and shoulders
CN XII Hypoglossal
Motor Control of the muscles of the tongue
nerve
Mid-Sagittal View
A mid-sagittal section slices the brain through the longitudinal fissure and separates the right
hemisphere from the left. It also reveals more structures. In a mid-sagittal view, all four cortical lobes
are visible. The frontal lobe is separated from the parietal lobe by the central sulcus, the occipital
256 | BRAIN ANATOMY
lobe is in the posterior region of the brain, and the temporal lobe can be seen behind the brainstem.
The cerebellum, pons, medulla, and spinal cord are seen caudal to the cerebrum, but in this view, the
midbrain—which is made up of two regions: the tegmentum and tectum—is also visible superior to
the pons. The corpus callosum is located in the center of the cerebrum and is a white matter bundle
made up of axons crossing from one hemisphere to the other. Surrounding the corpus callosum is the
cingulate gyrus, a region important for emotion.
Figure 23.11. A
midsagittal
section of the
brain. All four
cerebral lobes are
visible, as in the
cingulate gyrus,
which extends
through the
medial aspects of
the frontal and
parietal lobes.
The corpus
callosum sits
beneath the
cingulate gyrus.
Below the
cerebrum lies the
midbrain, pons,
medulla, and
cerebellum.
‘Internal Brain
Regions’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
The diencephalon region of the brain consists of the region around the thalamus and hypothalamus.
BRAIN ANATOMY | 257
It is located inferior to the fornix and lateral ventricle, posterior to the anterior commissure, and
superior to the brainstem. The fornix is a nerve fiber bundle containing primarily output from the
hippocampus. The anterior commissure sits above the hypothalamus and is a white matter tract (like
the corpus callosum) that allows information to cross from one hemisphere to the other. The thalamus
is best known for its role as a relay and processing location for the sensory and motor systems. The
hypothalamus has a variety of functions including control of stress and the “fight or flight” response of
the autonomic nervous system, reproduction, sleep, thirst, hunger, and other homeostatic functions.
The mamillary bodies sit in the posterior part of the hypothalamus and are important for memory. The
optic nerves from the retina cross at the optic chiasm and then the optic tracts continue back into the
diencephalon.
In the brainstem, the tectum of the midbrain consists of the superior and inferior colliculi, which
are important for vision and hearing, respectively. Finally, the reticular formation is located throughout
the brainstem. Networks within the reticular formation are important for regulating sleep and
consciousness, pain, and motor control.
258 | BRAIN ANATOMY
Figure 23.12.
Regions of the
diencephalon and
brainstem in a
midsagittal
section. The
thalamus,
hypothalamus,
and mammillary
bodies are part of
the
diencephalon.
The optic tracts
leave the
diencephalon,
cross at the optic
chiasm, and
continue as the
optic nerves out
to the retina. The
anterior
commissure and
fornix create the
front and upper
border of the
diencephalon.
The superior and
inferior colliculi
are part of the
midbrain tectum,
and the reticular
formation is
located
throughout the
brainstem.
‘Midsagittal
Diencephaon and
Brainstem’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BRAIN ANATOMY | 259
BY-NC-SA) 4.0
International
License.
Coronal Section View

Coronal sections of the brain allow deep tissue structures to be visible. A cut through the anterior
portion of the temporal lobe shows the amygdala, a region important for emotion, located in the
medial temporal lobe. The regions of the basal ganglia are also visible: the striatum (which consists of
the caudate and the putamen) and the globus pallidus. The basal ganglia has multiple functions, but is
best known for its role in regulation of movement. The lateral ventricle sits medial to the basal ganglia
and below the corpus callosum. The third ventricle is located in the middle of the brain, inferior to
the lateral ventricle. The longitudinal fissure separates the left and right cerebral hemispheres and the
lateral sulcus is the border between the frontal and temporal lobes.
260 | BRAIN ANATOMY
Figure 23.13 A
coronal section at
the location of
the amygdala.
The amygdala is
located in the
temporal lobe,
and the basal
ganglia is a
subcortical
structure located
near the lateral
ventricle. The
corpus callosum,
third ventricle,
longitudinal
fissure, and
lateral sulcus can
also be seen.
‘Amygdala and
Basal Ganglia’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
A coronal section taken closer to the central sulcus will make the hippocampus visible. The
hippocampus is known for its role in memory and spatial awareness. At this location, the basal ganglia
is more defined; the caudate and putamen are still present, but the two separate regions of the globus
pallidus (the internal and external segments) can be seen, as well as the subthalamic nucleus and the
substantia nigra. The thalamus is located on either side of the third ventricle. The corpus callosum
is superior to the lateral ventricle. The cerebrum is divided in half by the longitudinal fissure and the
lateral sulcus separates the temporal lobe from the frontal and parietal lobes.
BRAIN ANATOMY | 261
Figure 23.14. A
coronal section at
the location of
the
hippocampus.
The
hippocampus is
located in the
temporal lobe,
and the basal
ganglia is a
subcortical
structure located
lateral to the
thalamus and
lateral ventricle.
‘Hippocampus
and Basal
Ganglia’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Key Takeaways
• The four lobes of the cerebral cortex each have specific functions
• The cerebral cortex has gyri and sulci to increase the surface area
262 | BRAIN ANATOMY
• The cerebral cortex, underlying structures, cerebellum, brainstem, and spinal cord
form the central nervous system
• Cranial nerves are visible on the ventral surface of the brain
Test Yourself!
Attributions
Media Attributions
• Phineas Gage and his injury adapted by Valerie Hedges is licensed under a Public Domain license
• Sagittal section of brain structures © Casey Henley is licensed under a CC BY-NC-SA
• sagittal 2
• Amygdala and Basal Ganglia
BRAIN ANATOMY | 263
• Hippocampus and Basal Ganglia

264 | ADDITIONAL STRUCTURES OF THE NERVOUS SYSTEM
24.
ADDITIONAL STRUCTURES OF THE

NERVOUS SYSTEM
Although we mostly think about the neurons that make

up the brain and spinal cord as being the main characters
of the nervous system, there are many other anatomical
Resources features that play important supporting roles. These are
often non-neuronal structures that are still critically
important in allowing the nervous system to do what it
needs to do.
• Glossary Terms
• Key Takeaways
• Test Yourself Meninges
The brain is a soft and delicate internal organ housed
inside the skull. If there weren’t some protective buffer
separating the soft brain matter from the rigid bone, the jelly-like brain would be smashed up against
the inside of the skull and get injured as the head moves around.
The meninges are a series of protective membranes that minimize this kind of damage. They
surround the brain and extend all the way down the spinal cord. Think of the meninges as an organic
type of “bubble wrap” that encases a fragile nervous system.
There are three types of membranes that collectively make up the meninges. From the outermost to
innermost layer, they are:
1. Dura mater. The dura is made of thick, fibrous material and can get to be 0.8 mm thick in the
adult body (if you took a piece of printer paper and fold it in half four times, that should give
you an idea of how thick the dura mater is in the adult human). The dura mater is physically
attached to the inside of the skull with highly resilient connections found at the sutures between
ADDITIONAL STRUCTURES OF THE NERVOUS SYSTEM | 265
the plates of the cranium. The name originates from Latin meaning “tough mother”.
2. Arachnoid mater. The arachnoid mater is the middle layer of the meninges. The fibers are very
delicate and resemble a spider web, which is where the name comes from. Within this space,
there are protrusions that allow for CSF to drain into sinuses, which allow for recycling of
soluble substances. Most of the CSF in the brain exists underneath this layer in the subarachnoid
space.
3. Pia mater. The pia mater is the third layer of the meninges. It is very fragile, is in direct contact
with the surface of the brain, and closely follows the sulci and gyri. The name means “pious
mother”.
Figure 24.1. CNS

Meninges. There
are three
meninges that
cover both the
brain and the
spinal cord. The
outermost layer
beneath the skull
is the dura mater
(shown in
orange). Below
the dura mater is
the spider-web
like arachnoid
mater (light blue
and web-like).
The innermost
meninge is the
pia mater. The
pia mater (red) is
in direct contact
with the brain
and has a greater
surface area due
to it following
the gyri and sulci
of the brain.
‘Meninges’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Meningitis
Inflammation of the meninges is a potentially deadly condition called meningitis. Exposure

to infectious agents like viruses or bacteria such as Neisseria meningitidis (that leaks from
the blood into the meninges) is a common cause of the inflammation. When the meninges
are inflamed, the brain gets compressed from all sides, increasing intracranial pressure,
producing many of the same symptoms seen in hydrocephalus: fever, stiff neck, headache,
seizures, and altered mental status. The N. meningitidis bacteria and the viruses are highly
transmissible in close contact, but vaccinations are highly effective at minimizing the
infection rate. As with bacterial infections, broad-spectrum antibiotics are effective at
treating the infection.
Brain Circulation
Like every other organ in the body, the brain requires oxygen and nutrients to function. In humans,
this function is accomplished by the blood that is pumped around the body using a network of blood
vessels called the circulatory system. The brain has a very high demand for oxygen and nutrients: at
only 2% of total body weight, it receives about 15% of total cardiac output.
Stroke
Stroke is an extremely common, life-threatening medical condition that results in a loss of

blood flow to the brain. According to 2016 statistics from the World Health Organization,
stroke is the second-highest cause of death worldwide. The number one risk factor for
stroke is high blood pressure. There are two common types of strokes that a person may
experience.
More than 80% of all strokes are ischemic strokes (pronounced is-keemik), which
happens when normal blood flow is interrupted, causing cell death by deprivation of
oxygen and nutrients to brain tissue. Generally, this type of injury can happen when a blood
clot forms, travels through the circulatory system, and gets lodged in a tiny brain blood
vessel, thus, blocking the passage of blood.
The other 20% of strokes are hemorrhagic strokes, which result from a burst blood
vessel that causes bleeding into the brain. The presence of uncontrolled blood inside the
brain causes an increase in intracranial pressure, which can be lethal. Many brain cells may
die since they cannot take up oxygen directly from the blood. Additionally, blood has
dramatically different properties than the normal solution brain cells live in, and this can
cause the neurons to trigger a self-destruction program. Generally, hemorrhagic stroke is
more deadly than ischemic stroke.
Because the different blood vessels of the brain’s circulatory system are responsible for
providing blood to specific areas of the brain, it is possible to diagnose the specific area
where the stroke is happening based on the presentation of symptoms. For example, if the
middle cerebral artery blood is occluded by an ischemic stroke, the left hemisphere motor
cortex will lose blood flow. Because of the contralateral organization of the descending
motor pathway, the patient may therefore present with paralysis or weakness in the right
half of the body. It is vitally important to correctly diagnose and differentiate between the
two types of strokes. An ischemic stroke may be treated with injection of a “clot-busting”
drug, a substance that helps the body break down the offending blockage. However, these
clot-busters could make the bleeding from a hemorrhagic stroke even worse.
Figure 24.2. Types

of Stroke. A
hemorrhagic
stroke is caused
by a burst blood
vessel that
causes blood to
leak into
surrounding brain
tissue. An
ischemic stroke is
caused by a
blocked blood
vessel that does
not allow blood
to perfuse to
brain tissue.
‘Stroke’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
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Blood–Brain Barrier
It is important for oxygen and nutrients to pass from the blood into the brain tissue. Small blood
vessels outside of the brain, such as the capillaries in the fingertips, have very thin walls—sometimes the
width of a single cell—and are, therefore, highly permeable to gases. These vessels can either contain
tiny holes or large protein structures that physically transport substances across the blood vessel. On
the other hand, it is also advantageous to separate toxins and foreign pathogens in the bloodstream
from getting into brain tissue.
The blood–brain barrier (BBB) is an anatomical adaptation that selectively transports substances
necessary for normal biological function, while simultaneously excluding potentially harmful invaders
from the brain. The BBB physically surrounds blood vessels in the brain. It is made up of endothelial
cells and a type of glial cell called an astrocyte. The BBB is injured in a variety of medical disorders,
ranging from stroke, epilepsy, and Alzheimer’s disease, just to name a few. It is still unknown what
role the disruption of the BBB plays in brain disorders.
The exclusive nature of the BBB can be a double-edged sword. It is difficult to deliver a drug into the
brain from the blood stream if that drug is unable to pass through the BBB. For example, the current
gold standard pharmaceutical treatment for Parkinson’s disease is to increase the brain’s levels of
dopamine. However, dopamine does not pass through the BBB. To get around this, physicians give
the BBB-permeable substance L-DOPA, which the brain is able to convert into dopamine. Many other
therapeutic drugs do not cross the BBB, so researchers are developing methods using electromagnetic
fields to temporarily weaken the barrier, or surround the drugs in nanoparticles so small that the body
cannot identify them as foreign.
Figure 24.3.
Blood–Brain
Barrier.
Endothelial cells
in the body are
typically leaky
due to the
presence of gaps
and fenestrations
surrounding
blood vessels.
The endothelial
cells that
surround blood
vessels of the
brain have
astrocytes that
create tight
junctions that
restrict the
movement of
many substances
between the
blood and the
surrounding
tissues.
Ventricles
There are hollow spaces within the brain called ventricles. The human brain has a total of four
ventricles. The two very large, paired ventricles (one in each hemisphere) are the lateral ventricles.
They are connected medially to the third ventricle, which extends to the posterior aspect of the
brain. From here, the cerebral aqueduct that runs ventrally extends into the fourth ventricle before
continuing into the central canal: a narrow space that runs all the way through the length of the spinal
cord along the midline.
Figure 24.4.
Image of brain
ventricles. The
brain ventricles
(shown in blue)
are hollow areas
within the brain
that are
interconnected
and filled with
cerebrospinal
fluid. The
ventricles are
connected to the
central canal of
the spinal cord.
The ventricles are
show in a lateral
view (left) and
anterior view
(right).
The entire ventricular system is interconnected. The ventricles are filled with a liquid called
cerebrospinal fluid (CSF). CSF is basically a high-salt water solution. Because of the high osmolarity
of CSF, it is a very buoyant solution. Like a fully grown person who can float easily on the surface of
the extremely salty Dead Sea, CSF allows the brain to remain “floating” inside the skull. Without CSF,
the brain weighs almost 1.5 kg (~3 lbs). Cells and blood vessels at the ventral base of the brain would
be crushed under the weight of the brain itself. But when the brain is surrounded by CSF, it weighs less
than 50 grams, almost two orders of magnitude lighter!
CSF is also found within the meninges that encase the brain. In fact, more than 80% of the CSF in
the body exists in this space outside the brain. This liquid serves as a form of “cushioning” that protects
the brain from rapid head movements. If it weren’t for this physical protection, the inertia of head
movement may cause your brain to smash against the inside of the rigid skull if you move your head
too quickly.
The CSF layer allows the head to withstand some sloshing of the brain, but a movement that is too
abrupt can cause a traumatic brain injury. CSF can also function as a way to wash impurities out
of the brain. The volume of CSF in the typical human body is about 150 mL, a little more than half a
cup. Because there is frequent turnover of CSF, the material gets absorbed back into the body regularly.
Each day, the body produces about half a liter of CSF, so the brain cycles through the entire volume a
few times.
Hydrocephalus
Hydrocephalus, historically called “water on the brain”, is a common condition affecting the
brain of about 1 in 200 newborns and a small number of adults. In patients with
hydrocephalus, the volume of CSF increases, which elevates intracranial pressure, causing
symptoms such as fever, stiff neck, headache, seizures, or altered mental status.
In adults, the skull is rigid and unmoving. But in newborns with hydrocephalus, the plates
of the skull are not completely fused together. Often, these children will have a bulging
parts on the skull and an expansion of the forehead.
Increased CSF volume can happen in a couple ways. The clearance of CSF may fail while
production remains normal, or the entrance to the central canal in the spinal cord may be
narrowed or blocked by a tumor, leading to an increase in the volume in the brain. A
common treatment for hydrocephalus is to surgically implant a shunt (a hollow tube that
runs from the ventricle down into the abdominal space) that allows for drainage, thus
decreasing intracranial pressure.
Figure 24.5.
Hydrocephalus.
The typical infant
has normally
sized ventricles
(shown in dark
purple) in the left
image. The infant
with
hydrocephalus
has enlarged
ventricles and a
bulging forehead
(right image).
‘Hydrocephalus’
by Valerie Hedges
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Key Takeaways
• The meninges are protective coverings that cover central nervous system structures
• There are two types of stroke: ischemic (blocked blood vessel) and hemorrhagic
(burst blood vessel)
• Ischemic strokes account for 80% of strokes and hemorrhagic strokes account for
20% of strokes
• The blood–brain barrier surrounds blood vessels of the brain to restrict the
substances that can enter the brain
• Ventricles are hollow spaces in the brain that are filled with cerebral spinal fluid
Test Yourself!
Attributions
Media Attributions
• Meninges © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial

ShareAlike) license
• Stroke © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Blood Brain Barrier © Armin Kübelbeck adapted by Valerie Hedges is licensed under a CC BY
• Brain Ventricles © Bruce Blaus adapted by Valerie Hedges is licensed under a CC BY
• hydrocephalus © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
IMAGING THE LIVING BRAIN | 277
25.
IMAGING THE LIVING BRAIN
The earliest methods of analyzing nervous system

anatomy were crude: manual dissection of the brain post-
mortem (after death). The techniques discussed below
were developed to allow scientists to see some aspect of the Resources
anatomy of the nervous system—either gross anatomical
differences or connectivity. Additional techniques were
developed to see how the living brain functions.
• Glossary Terms
• Key Takeaways
Studying Brain Structure • Test Yourself
The following techniques are used to analyze brain

structure.
CT Scan
Example questions answered:
“Does the patient have a brain tumor and where is the brain tumor located?”
“Are the meninges intact?”
The CT scan relies on X-ray technology. X-rays are high energy beams of electromagnetic radiation
that are capable of passing through many physical objects. Traditional two-dimensional X-rays, such
as those used to image a broken bone or tooth decay, use radiographic film to detect where the X-rays
get blocked. When an X-ray passes unimpeded, it causes the film to darken. But, wherever the X-rays
are blocked, the film remains white. Therefore, material that is more dense (such as bone) appears as
white, less dense material (such as the air surrounding the body or CSF) appears dark. Other tissue are
some shade of gray in between.
278 | IMAGING THE LIVING BRAIN
The CT scan is essentially a three-dimensional X-ray that revolves around the person as they move
through the scanner. Instead of using radiographic film, the CT scan uses a computer that detects
the passage of X-rays, located directly across the emission source of the X-ray. Instead of a flat, two
dimensional-image, the CT scan uses an X-ray gun that revolves around the person’s body as they
advance slowly through a large circular hole. The computer is then able to compile the series of two-
dimensional images and turn them into a three-dimensional reconstruction that can be used to see the
brain from any projection. CT scans give us a spatial resolution of about 0.5 mm.
CT scans are generally used clinically to assess diagnostic changes over several days (such as before
and after tumor removal or to determine if an intracranial bleed has healed), so temporal resolution is
not a major consideration. As an anatomical analysis that can easily identify tissues of different density,
it is great for identifying and diagnosing particular brain conditions. Brain tumors can be visualized
in a CT scan, since they are identified by an increase in tissue density compared to normal brain
tissue. Hydrocephalus, an abnormal and potentially deadly expansion of the CSF-filled ventricles, can
be quickly identified by this analysis. Meningitis, an inflammation of the meninges, may present as
increased contrast in the CT scan.
The big advantage of the CT scan is that it is noninvasive. You can use a CT scan in order to
diagnose and identify the cause of a condition while a person is still alive, and hopefully work towards
developing an intervention. It is also a relatively quick technique. A full-head CT scan takes only
minutes, which allows for a rapid diagnosis of anatomical structures. However, X-rays are highly
mutagenic. Prolonged exposure to X-rays dramatically increases the risk of developing various cancers,
since X-rays interfere with the process of DNA replication. It is estimated that the radiation exposure
in a single head CT scan is similar to the background exposure of X-rays in a few months. When a CT
scan is prescribed, the diagnostic information gained from a CT scan is more important than the risks
from increased radiation exposure.
Figure 25.1. A CT
scan (or CAT
scan) uses X-ray
technology to
produce an
image of brain
structures. CT
scans are
non-invasive and
are useful for
visualization of
the brain
ventricles or
brain tumors. In
the above
images are
horizontal
sections of a
human brain
with brain
tumors that are
shown as
lightly-colored
areas of tissue.
Magnetic Resonance Imaging (MRI)

“Are their structural abnormalities in a brain structure?”
“What are the measurements of a brain structure?”
Magnetic resonance imaging (MRI) is used for imaging brain structures. An MRI is able to more
clearly differentiate between different types of tissues with high resolution. It can be used in place of
CT scan for visualization of structures that are very small or need finer detail than can be seen with a
CT scan.
The MRI machine is a circular tunnel through which a person on a table moves. As the person
moves through the scanner, an extremely powerful magnet revolves around their head. The power of
a typical magnet used in a hospital MRI may be as powerful as 10,000 gauss (1 Tesla)—strong enough
to lift a car. The more powerful fMRI machines can be as powerful as 100,000 gauss (10 Tesla). The
stronger the magnets, the better the spatial resolution that the machine can produce (our current best
spatial resolution is on the order of millimeters).
MRI technology detects and quantifies the movement of water molecules, which moves differently
in gray matter than white matter. The movement of a water molecule in biological tissue is not
completely random, largely because the brain is made up of heterogeneous tissue.
Although the technique has a great capacity for analyzing brain structure, the nature of the machine
itself presents limitations. The machine can also be very loud, which is not trivial if you are interested in
using this technology on younger patients. The use of a tremendously powerful magnetic field presents
a different set of limitations. At the risk of severe injury or death, the patient entering the scanner
cannot have any magnetosensitive implants, such as metallic aneurism clips, intrauterine devices, or
shrapnel. Even older generation tattoos have trace amounts of metal that cause burns when exposed to
the magnets of the MRI machine.
Figure 25.2. MRI Scan. An MRI scan uses high-powered magnets to

produce high resolution images. MRI scans can clearly distinguish brain
structures with higher resolution than other imaging techniques like CT
scans. In this image of a midsagittal human brain fine details of brain
structures can be visualized.
Diffusion tensor imaging (DTI)

“Is the volume of the white matter tract medial longitudinal fasciculus important for normal language
processing?”
“Does spinal cord compression cause neurological deficits?”
While a CT scan is great for detecting gross anatomical anomalies like tumors or intracranial
bleeding, it has a difficult time with subtle anatomical changes like differences between gray matter
and white matter tracts. A technique for identifying these differences was proposed in 1994, called
diffusion tensor imaging (DTI).
DTI quantifies white matter because of the morphological features of white matter. More
specifically, DTI uses MRI technology to detect and quantify the movement of water molecules, which
move differently in gray matter than white matter. The movement of a water molecule in biological
tissue is not completely random, largely because the brain is made up of heterogeneous tissue. White
matter is very different from gray matter. A water molecule is more easily able to diffuse along the same
direction as a tract of white matter but has a difficult time moving perpendicularly across such tissue.
When looking at DTI images, they will have coloring added to the figure to show the different white
matter tracts.
Axonal projections are directional, with the soma at one end and the axon terminal at the other. One
of the shortcomings of DTI is that it cannot give us information about the directionality of the axonal
projections.
Figure 25.3. Diffusor Tensor Imaging. DTI imaging uses MRI technology to detect and
quantify the movement of water molecules along tracts of white matter. This
techniques allows for the visualization of white matter connections within the brain.
The coloring is added to the image to show the different white matter tracts.
Studying Brain Function

The following techniques are used to study brain function.
Positron emission tomography (PET scan)

“Which areas of the brain decrease in activity when a person experiences mild cognitive impairment?”
“Do drug-dependent people have a high density of opioid receptors?”
The positron emission tomography (PET) scan is an application of nuclear medicine best known
for its applications in the medical setting for the diagnosis of cancer. Before the PET scan, a radioactive
compound called a tracer is injected into the bloodstream. The PET scanner itself is a large circular
device that looks similar to the CT scanner. The tracer is chemically unstable, and it produces a signal
within the body that can be detected by the PET scanner as the person moves through the machine.
A common tracer is a radioactive analog of glucose. Areas of the brain that are active will metabolize
glucose for energy. Therefore, when an area of the brain increases in energetic demand, that change can
be detected by identifying the increase in glucose movement.
PET scans can be effective at diagnosing and identifying the location of tumors in the nervous
system. It also provides an overall picture of brain activity, which may be useful in diagnosing disorders
of cognitive deficits, like dementia associated with Alzheimer’s disease. PET scans have also been used
to image the activity of specific brain areas as a person performs behavioral tasks, but this use of PET
scan has largely been replaced by functional magnetic resonance imaging. Another application of PET
scanning is to visualize levels of receptors in vivo.
When looking at PET images, they will have coloring added to the figure to show
where, anatomically, activity in the brain has increased or decreased. By convention, many times an
increase in brain activity is depicted with warm colors (yellow, orange, red) whereas a decrease in brain
activity is depicted with cool colors (blue, purple).
The downside of the PET scan as a diagnostic tool is similar to a limitation of the CT scan. A person
is exposed to radioactive compounds and gamma wave radiation, which are potentially mutagenic. In
images produced by a PET scan, it is often difficult to identify boundaries between tissue, even between
dramatically different internal organs. To make up for this deficit, PET scans are frequently performed
simultaneously with an anatomical analysis like a CT scan. PET scans generally have very poor spatial
and temporal resolution.
Figure 25.4. PET Scan Images. The positron emission tomography (PET) scan is used to
visualize brain activity. A radioactive tracer is injected into the bloodstream prior to scanning
that can be detected by the scanning machine. PET scans are colored to show where in the
brain is experience increases or decreases in brain activity. Typically, increased brain activity is
indicated with warm colors (yellow, orange, red) and decreased brain activity is depicted with
cool colors (blue, purple).
Functional magnetic resonance imaging (fMRI)

“Do neurons in the right hemisphere cingulate gyrus increase in activity when a person sees their loved
ones?”
“Which areas of the brain change in activity when a person is planning a motor action?”
The functional magnetic resonance imaging (fMRI) technique is probably the most well-known
method of studying brain activity. Because fMRI can be performed while a person is engaged in a task,
many research studies use fMRI as a means to correlate behavior with activity patterns in specific parts
of the brain.
fMRI measures differences in blood flow and oxygen levels. Like the PET scan, the fMRI hinges
on the idea that more active areas of the brain have different metabolic demands than less active areas
of the brain. When there is more activity in one area of the brain, the neurons in that area need more
oxygen. This change in blood flow is detected by the fMRI. The main reason fMRI is useful in so many
research applications is that you are able to visualize brain activity real-time during the performance
of complex behavioral tasks. You can present specific visual stimuli to a person in an fMRI scan
and evaluate which parts of the brain change in activity. For example, seeing pictures of faces causes
increased blood flow into the fusiform face area. You can ask a person to perform a gambling task and
evaluate the areas of the prefrontal lobe that are responsive to risk taking.
When looking at fMRI images, they will have coloring added to the figure to show anatomically
where activity in the brain has increased or decreased. By convention, many times an increase in
brain activity is depicted with warm colors (yellow, orange, red) whereas a decrease in brain activity is
depicted with cool colors (blue, purple).
The limitations introduced with MRI earlier are the same for fMRI. In addition, due to the small
tube, it can be difficult to study anxiety with endangering the patient. Further, the data collected by
fMRI can be very difficult to analyze and are frequently subject to false positives. fMRI also assumes
that increased blood flow is directly correlated with the amount of neural activity, which may not
always be the case.
Figure 25.5. fMRI

Scan Images. An
fMRI scan
measures
differences in
blood flow and
oxygen levels,
similarly to an
MRI scan. An
fMRI, however,
can be used
while an
individual is
performing a
task to look at
changes in brain
activity over
time. Coloring is
added to fMRI
images to show
where within the
brain there is
increased or
decreased brain
activity. Similar to
PET scans,
increases in brain
activity are
depicted with
warm colors
(yellow, orange,
red) whereas
decreases in
brain activity are
depicted with
cool colors (blue,
purple).
Key Takeaways
• CT scans, MRI scans, DTI are used to visualize brain structures

• PET scans and fMRI scans are used to visualize brain function
Test Yourself!
Attributions
Media Attributions
• CT scan © Jmarchn adapted by Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

• MRI scan © deradrian adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• DTI image © Thomas Schultz is licensed under a CC BY-SA (Attribution ShareAlike) license
• PET scan © Health and Human Services Department, National Institutes of Health, National
Institute on Aging : p.24 is licensed under a Public Domain license
• fMRI scan © John Graner is licensed under a Public Domain license
290 | SPINAL CORD STRUCTURE
26.
SPINAL CORD STRUCTURE
It is sometimes easy to think of neuroscience as a focused

study of the brain: How does activity of the brain
contribute to behavior? In what ways does the brain
Resources change in disease? Why do the cells of the brain behave the
way they do?
The truth is there are many parts of the body that also
fall under the broad study of neuroscience. For example,
• Glossary Terms the automatic kneejerk reflex that a clinician examines
• Key Takeaways when they tap on your patellar tendon is a test of the
• Test Yourself nervous system. The reflex is driven by sensory neurons
that detect muscle stretch, motor neurons that cause the
kicking response, and interneurons that prevent the
opposing muscle from acting. We have neural circuits that
provoke changes in the activity of our internal organs, from the beating of our heart to the digestion of
food, and the study of these systems is certainly part of neuroscience as well.
Moving posterior from the brainstem is the other organ of the central nervous system: a long, thin
structure of nervous tissue called the spinal cord. It functions to carry information both upwards
towards the brain and downwards towards the body’s other organs and muscles. It can also process
sensations and form an appropriate motor response in the absence of brain input.
Spinal Column Anatomy

The spinal cord begins at the base of the brainstem and runs down to the small of your back, giving
it a length around 44 cm (17.5 inches). The spinal cord is housed within a series of bones, called the
vertebral column. Although the spinal cord itself is continuous, it can be divided based on the overlying
SPINAL CORD STRUCTURE | 291
vertebrae. A combination of a letter and a number is used to identify each section of the spinal cord;
the letter corresponds to the vertebral section and the number refers to the number of bones down
from the previous section (the smaller numbers are more anterior, larger numbers more posterior).
The diameter and shape of the spinal cord changes over the length of vertebral column, a result of the
function of the spinal nerves. For example, the region where motor neurons are located (ventral horn)
is larger in the cervical region of the spinal cord compared to other regions of the spinal cord with
minimal motor output.
Branching off from each section of the spinal cord are two pairs of nerves, the afferent (incoming to
the CNS) sensory nerve roots, which branch from the dorsal side of the spinal cord, and the efferent
(outgoing from the CNS) motor nerve roots, which branch from the ventral side of the spinal cord.
These two branches meet and extend away from the spinal cord. After merging, they are called the
spinal nerves.
The vertebral column is divided into four main regions: cervical, thoracic, lumbar, and sacral. The
spinal cord and spinal nerves that enter and exit the vertebral column are divided into these regions as
well. Moving from anterior (top) to posterior (bottom), the four regions of the spinal cord are:
1. Cervical. The cervical region corresponds to C1 through C7. Nerves that exit through the
cervical region innervate the muscles in the neck, shoulders, arms, and hands. Afferent nerves
detect somatosensory inputs from these same areas. Sections C3 through C5 innervate the
diaphragm, so an injury at this level or higher can quickly lead to death since the person may
stop breathing. The spinal cord is at the widest diameter at the cervical area, as it has a swelling
that corresponds to the many inputs and outputs to the arms.
2. Thoracic. The thoracic region corresponds to T1 through T12. These regions innervate the
middle trunk area, the intercostal muscles between the ribs, and abdominal muscles. Branches of
the spinal nerves in the thoracic areas are responsible for changing the activity of the various
internal organs during a fight-or-flight response (more on the autonomic nervous system in
Chapter 27).
3. Lumbar. The lumbar region corresponds to L1 through L5. These pathways carry motor
command information to the hips, thighs, and knees. Afferent lumbar inputs detect sensory
information from the ventral side of the legs, such as the top of the thigh or the shin bone. As in
the cervical region, the lumbar region has a swelling that increases the diameter of this section of
spinal cord compared to the thoracic or sacral areas.
4. Sacral. At the posterior-most end of the spinal cord is the sacral region, which corresponds to S1
through S5. Sacral spinal nerves control flexing of the toes. These nerves detect sensory
information around the genital organs and the dorsal aspects of the legs, like the buttocks and
the back of the thighs. There are also parasympathetic nerves that come from the sacral region
and these innervate the colon, bladder, and genital organs (more on the autonomic nervous
system in Chapter 27). Since information must pass through the anterior regions of the spinal
cord to reach the posterior parts of the body, the more anterior an injury, the more parts of the
body that are affected.
Figure 26.1. The

vertebral column
and
representative
spinal cord
cross-sections.
The vertebral
column and
corresponding
spinal cord and
spinal nerves are
divided into four
regions. The
cervical division
is the most
rostral, starting
at the base of
the brainstem.
The thoracic is
the largest
division, just
caudal to the
cervical. The
lumbar is the
next division, and
the sacral is the
most caudal.
‘Spinal Cord’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
From L2 through S5, the spinal cord exists as the cauda equina, meaning “horse’s tail” due to the
appearance of the cord as individual nerves that branch from the main spinal cord. These nerves then
innervate the pelvis area and lower limbs. This area of the spinal cord is where spinal tap and epidural
procedures take place due to the decreased risk of spinal injury.
Figure 26.2.
Cauda equina.
The spinal cord at
the L2-S5 levels
is called the
cauda equina. At
the cauda equina,
the spinal cord
branches into
individual nerves
that innervate
the pelvis area
and the lower
limbs.
The spinal cord is part of the central nervous system, but the fibers that leave and enter the spinal cord
are located in the peripheral nervous system. These spinal nerves can then extend to or from target
tissues throughout the body.
Figure 26.3. The

spinal cord is part
of the central
nervous system,
but the axons
that exit and
enter the spinal
cord are in the
peripheral
nervous system.
‘Spinal Cord CNS
and PNS’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Nerves are collections of neuron axons found within the peripheral nervous system and can be
classified as sensory, motor, or mixed nerves. The dorsal root is an example of a sensory (afferent) nerve
that is responsible for carrying information toward the central nervous system. The ventral root is an
example of a motor (efferent) nerve that is responsible for carrying information away from the central
nervous system. Most nerves in the body are classified as mixed nerves that contain both sensory and
motor fibers.
Figure 26.4.
Sensory, or
afferent nerves,
contain only
sensory axons,
which can be
found at the
dorsal root. of
the spinal cord.
Motor, or
efferent, nerves
contain only
motor axons and
can be found at
the ventral root
of the spinal
cord. Most
nerves within the
body contain
both sensory and
motor neurons
and are referred
to as mixed
nerves. ‘Nerve
Classification’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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Cross Section Anatomy of the Spinal Cord

Like the brain, the spinal cord is also made up of regions of white matter and gray matter. White matter
regions are comprised of axons. It appears white due to the myelin sheath on the axons. Gray matter
regions are comprised of cell bodies and dendrites. Gray matter is the location of most synapses.
In cross section, the gray matter of the spinal cord is found medially, and the white matter is found
laterally. When referring to the spinal cord, we will typically use the directional terms “dorsal” and
“ventral”. By convention, when looking a cross section of the spinal cord (a horizontal cut through
the cord), the dorsal portion of the spinal cord will be located at the top of the image and the ventral
portion of the spinal cord will be located at the bottom of the image. There are a few structures to be
aware of when examining the spinal cord in cross section.
Figure 26.5. The

spinal cord can
be divided into
white and gray
matter. White
matter is
primarily
myelinated
axons. Gray
matter is
primarily
neuronal cell
bodies and
dendrites. In the
spinal cord, the
inner part is gray
matter, whereas
the surround
tissue is white
matter. The
regions that
extend from the
central nervous
system and into
the peripheral
nervous system
primarily
contains axons
traveling to or
from peripheral
targets and,
therefore, are
mainly white
matter except for
ganglia, which
are clusters of
cell bodies in the
periphery. ‘Spinal
Cord White and
Gray Matter’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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The white matter in the spinal cord is divided into structures called columns because the axons in
these regions are either ascending toward the brain or descending toward the appropriate spinal nerve.
The dorsal column is on the dorsal or posterior side of the spinal cord, the ventral horn is on the
ventral or anterior side of the spinal cord, and the lateral column lies between them. The gray matter
is likewise divided into regions called horns. The dorsal horn is the location of sensory synapses, the
ventral horn is the location of motor neuron cell bodies, and the lateral horn is the location of cell
bodies of the autonomic nervous system. The dorsal root and ventral root consist of the axons of
afferent (dorsal) and efferent (ventral) fibers. They combine to form the spinal nerves. Sensory neuron
cell bodies are located in the dorsal root ganglion, a gray matter region of the dorsal root.
Figure 26.6. The

spinal cord is
comprised of
white and gray
matter. The
dorsal column
and dorsal horn
are on the
posterior side of
the spinal cord.
The ventral
column and
ventral horn are
located on the
anterior side of
the spinal cord.
The lateral
column and
lateral horn are
located in the
middle. The
spinal nerves
that extend into
the periphery
consist of fibers
that split into the
dorsal root and
the ventral root
to enter (dorsal)
or exit (ventral)
the spinal cord.
The dorsal root
ganglion is a gray
matter region of
the dorsal root.
‘Spinal Cord
Anatomy’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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All somatosensory receptor neurons have their cell bodies located in the dorsal root ganglion; a
structure found just outside the dorsal aspect of the spinal cord. The receptor neurons (also called
primary afferent fibers) of the somatosensory system are bipolar neurons, meaning they have one
process from the cell body that splits into two branches. Afferent fibers coming from the periphery
through the spinal nerves enter the spinal cord via the dorsal root. The cell bodies of sensory neurons
are located in the dorsal root ganglion. The axons continue into the spinal cord and typically synapse in
the dorsal horn. Interneurons are very short neurons that are a communication link between cell types
in the spinal cord. They can be either excitatory or inhibitory depending on their role. They can also
cross the midline of the spinal cord. The cell bodies of motor neurons that innervate skeletal muscles
are located in the ventral horn. The efferent axons of these neurons leave the spinal cord via the ventral
root and then enter the spinal nerve on their way to their target tissue.
The ventral portion of the spinal cord is concerned with motor output, or efferent signals. Muscle
fibers are innervated by alpha motor neurons that have their cell bodies in the ventral horn of the
spinal cord. Their axons leave the spinal cord via the ventral roots and travel to the muscle via efferent
peripheral spinal nerves.
In summary, sensory information is concerned with the dorsal portion of the spinal cord whereas
motor information is concerned with the ventral portion of the spinal cord.
Figure 26.7.
Afferent axons
coming from the
periphery travel
through the
dorsal root to
enter the spinal
cord. These
axons can
synapse on
interneurons,
cells with short
axons that
communicate
with other cell
types. Efferent
fibers, like those
of the skeletal
muscle motor
neurons located
in the ventral
horn, leave the
spinal cord
through the
ventral root.
‘Spinal Cord
Fibers” by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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Key Takeaways
Type your key takeaways here.
• There are four regions of the spinal cord

• There are gray matter and white matter areas of the spinal cord
• Sensory information enters into the spinal cord via the dorsal root ganglion to the
dorsal horn
• Sensory cell bodies are located within the dorsal root ganglion
• Motor information exits the spinal cord from the ventral horn then through the
ventral root out to the body
Test Yourself!
Attributions

Media Attributions
• Veretbral Column
• Cauda equina © John A Beal adapted by Valerie Hedges is licensed under a CC BY (Attribution)
license
• Spinal Cord CNS and PNS
• Nerve Classifications
• Spinal Cord White and Gray Matter
• Spinal Cord Anatomy
• Spinal Cord Fibers
304 | PERIPHERAL NERVOUS SYSTEM
27.
PERIPHERAL NERVOUS SYSTEM
The Peripheral Nervous System (PNS) functions as the

intermediary between the central nervous system
(CNS) and the rest of the body, including the skin,
Resources internal organs, and muscles of our limbs. The PNS can
be divided into three main branches:
1. Somatic nervous system

• Glossary Terms 2. Autonomic nervous system
• Key Takeaways 3. Enteric nervous system
• Test Yourself
In this chapter, the somatic and autonomic nervous
systems will be compared and contrasted. An overview of
the enteric nervous system will also be provided.
Somatic vs. Autonomic Nervous System

The somatic and autonomic nervous system differ in their:
• Target/effector organs
• Efferent pathways and the neurotransmitters that are used
• How the target/effector organ responds to the neurotransmitter
PERIPHERAL NERVOUS SYSTEM | 305
Peripheral Nervous System: Somatic Nervous

System
The somatic nervous system represents all the parts of the PNS that are involved with the outside
environment, either in sensing the environment or acting on it. For example, the nerves that detect
pressure or pain on the foot are part of the afferent somatic nervous system. We also think of the
somatic nervous system as the branch that sends signals to our skeletal muscles. The nerves that
innervate the muscles of the legs as we run are part of the efferent somatic nervous system. The somatic
nervous system is also called the “voluntary nervous system” since it is used to cause muscle movement
related to intentional actions.
The somatic nervous system has an efferent path from the central nervous system to the target /
effector organ that is made up of one neuron. The axon of this neuron is heavily myelinated, which
allows for fast delivery of messages. This somatic motor neuron has its cell body in the central nervous
system and has an axon that extends all the way to the target/effector organ, which—for the somatic
nervous system—will be a skeletal muscle.
Figure 27.1.
Somatic nervous
system efferent
pathway. The
somatic nervous
system efferent
path consists of a
neuron that has
a cell body within
the central
nervous system
that extends all
the way to the
target tissue:
skeletal muscles.
The
neurotransmitter
released onto the
skeletal muscle is
acetylcholine.
‘Somatic Nervous
System’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
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4.0 International
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Synapse: Neuromuscular Junction

All somatic motor neurons release the same neurotransmitter, acetylcholine, onto the skeletal muscle
target/effector organ at the neuromuscular junction (NMJ). The NMJ is one of the largest synapses
in the body and one of the most well-studied because of its peripheral location. Acetylcholine is the
neurotransmitter released at the NMJ and it acts upon ligand-gated, non-selective cation channels
called nicotinic acetylcholine receptors that are present in postjunctional folds of the muscle fiber.
Acetylcholinesterase, an enzyme that breaks down acetylcholine and terminates its action, is present
in the synaptic cleft of the neuromuscular junction.
Figure 27.2. The neuromuscular junction (NMJ) is the synapse between a motor neuron and a muscle
fiber. Acetylcholine is released at the NMJ and acts on nicotinic acetylcholine receptors located in the
postjunctional folds of the muscle fiber. Neurotransmitter action is terminated through breakdown
by acetylcholinesterase. ‘Neuromuscular Junction’ by Casey Henley is licensed under a Creative
Nicotinic acetylcholine receptors allow for the influx of sodium ions into the muscle cell. The
depolarization will cause nearby voltage-gated channels to open and fire an action potential in the
muscle fiber. In a healthy system, an action potential in the motor neurons always causes an action
potential in the muscle cell. The action potential leads to contraction of the muscle fiber.
Figure 27.3. The ionotropic nicotinic acetylcholine receptors in the postjunctional folds of the muscle
fiber are non-selective cation channels that allow the influx of sodium and the efflux of potassium.
The depolarization of the cell by the sodium influx will activate nearby voltage-gated ion channels.
‘NMJ Ion Flow’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Peripheral Nervous System: Autonomic Nervous

System
The autonomic nervous system encompasses all the branches of the peripheral nervous system that
deal with the internal environment. As with the somatic nervous system, the autonomic nervous
system is comprised of nerves that detect the internal state as well as nerves that influence the internal
organs. The body carries out all sorts of functions and responses unconsciously without any
intentional control. It can do so by sending signals to smooth muscles and glands. The signals that
cause us to sweat when it is hot, our pupils to dilate when it is dark, and our blood pressure to adjust
when we stand up too quickly are all driven by the nerves of the autonomic nervous system.
The autonomic nervous system has an efferent path from the central nervous system to the target/
effector organ that is made up of a chain of two neurons with a ganglion in the middle. Recall that a
ganglion is a collection of neuron cell bodies in the periphery.
The neuron that has its cell body in the central nervous system is called the preganglionic neuron.
The preganglionic cell axon is lightly myelinated and extends to a ganglion in the periphery where it
synapses on the second neuron in the chain called the postganglionic neuron. The postganglionic
neuron has its cell body in the ganglion and its unmyelinated axon extends from the ganglion to the
target/effector organ, which for the autonomic nervous system will be: smooth muscle, cardiac muscle,
glands, or organs.
The first synapse happens at the site of the ganglion. All preganglionic cells release acetylcholine
onto the postganglionic cells. The acetylcholine binds to nicotinic acetylcholine receptors, which
results in the generation of an excitatory postsynaptic potential for the postganglionic cell body.
This effectively passes the message between the preganglionic and postganglionic cells.
The second synapse occurs between the postganglionic cell and the target/effector organ.
Postganglionic neurons in the autonomic nervous system release either norepinephrine or
acetylcholine (more on this later). These neurotransmitters can have either stimulatory or inhibitory
activity at the target organs, dependent on the properties of the postsynaptic receptor that they bind
to.
Fig 27.4.
Autonomic
nervous system
efferent path.
The efferent path
for the
autonomic
nervous system
consists of two
neurons. The first
neuron is called
the preganglionic
neuron and it
starts in the
central nervous
system and
extends to a
ganglion in the
periphery where
it releases
acetylcholine
onto the
postganglionic
neuron. The
postganglionic
neuron extends
to the target
tissue where it
releases either
acetylcholine or
norepinephrine.
Target tissues
include smooth
muscle, cardiac
muscle, glands,
and organs.
‘Autonomic two
neuron chain’ by
Valerie Hedges is
licensed under a
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Autonomic Effector Synapse

Within the autonomic nervous system, synapses have a different structure than what is observed at
the NMJ of the somatic nervous system. Instead, autonomic axons are highly branched and have
enlarged varicosities that are spread along the axon. These varicosities contain synaptic vesicles that
are filled with neurotransmitters. The varicosities form synapses ‘en passant’ (literally meaning ‘in
passage’) with the target organ. These axon branches with varicosities drape over the cells of the target
tissue, allowing a single axon branch to affect change over a greater area of the target tissue and better
distribute autonomic activation at the target tissue.
Figure 27.5. An autonomic axon branches extensively to cover effector tissues like a net. Multiple
varicosities have synaptic vesicles that release neurotransmitters onto effector cells. ‘Synapse en
passant’ by Valerie Hedges is licensed under a Creative Commons Attribution Non-Commercial
Comparing Somatic to Autonomic Nervous

System
In summary, the somatic nervous system has a one neuron path that originates in the central nervous
system. That neuron extends all the way to the skeletal muscle (target tissue) where it releases
acetylcholine. The acetylcholine binds to nicotinic acetylcholine receptors that cause EPSPs in the
muscle fibers and giving a stimulatory effect. The autonomic nervous system (both branches) use a
two neuron pathway between the CNS and the target tissues that are smooth muscle, cardiac muscle,
glands, and organs. The sympathetic division consists of short presynaptic neurons that synapse on
longer postsynaptic neurons in peripheral ganglia that are situated close to the spinal cord. The
parasympathetic division consists of long presynaptic neurons that synapse on shorter postsynaptic
neurons in peripheral ganglia that are situated close to the target organ. Like the somatic motor system,
the primary neurotransmitter in the parasympathetic division is acetylcholine. In the sympathetic
division, the preganglionic neuron releases acetylcholine, but the postganglionic neuron releases
norepinephrine. The effects of these neurotransmitters at the target tissue can be either stimulatory or
inhibitory depending on the properties of the postsynaptic receptor. This information is summarized
in the figure and table below.
Figure 27.6. The

somatic motor
system consists
of single neuron
that extends
from the central
nervous system
directly to
skeletal muscle
where it releases
acetylcholine.
The autonomic
nervous system
uses a
two-neuron
pathway. The
preganglionic cell
extends from the
central nervous
system to a
peripheral
ganglion where it
synapses on the
postganglionic
cell body and
releases
acetylcholine.
The
postganglionic
neuron then
innervates the
target tissue,
which could be
smooth muscle,
cardiac muscle, or
a gland. In the
sympathetic
division, the
postganglionic
neuron releases
norepinephrine
onto the target
tissue, whereas
in the
parasympathetic
system, the
postganglionic
neuron releases
acetylcholine
onto the target
tissue. ‘Somatic
vs SNS and PNS
Motor’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Table 27.1. Table comparing the somatic nervous system to the autonomic nervous system specifically
identifying the efferent path, the target / effector organ, the neurotransmitter released at the ganglion
and effect, the neurotransmitter released at the target organ, and the response of the target organ to
the neurotransmitter.
Neurotransmitter
Neurotransmitter Target Organ
Branch of Efferent Target / Released at
Released at Response to
PNS Path Effector Organ Ganglion and
Target Organ Neurotransmitter
Effect
1 None- No
Somatic Skeletal Muscle Acetylcholine Stimulatory
Neuron Ganglion
2 Smooth /
Acetylcholine, Acetylcholine or Can be stimulatory
Autonomic Neuron Cardiac Muscle,
excitatory Norepinephrine or inhibitory
Chain Glands, Organs
Two Branches of the Autonomic Nervous System

There are two branches of the autonomic nervous system, called the sympathetic nervous system and
the parasympathetic nervous system, that typically have opposite effects at target tissues.
Consider a scenario in which you encounter a bear on hike through Yellowstone National Park.
Your heartrate would likely increase and your breathing rate would increase as you contemplate how
to survive your encounter. This complex set of physiological reactions are due to activation of one of
the branches of the autonomic nervous system: the sympathetic nervous system. The sympathetic
nervous system mobilizes a set of physiological changes to a threat that is sometimes called the fight-or-
flight response, which is activated when we are faced with a threat, either perceived or real. All of these
rapid bodily responses result in the body preparing to attack or defend itself. Increased respiration
allows the body to take in more oxygen, and dilation of blood vessels in the muscles allows that oxygen
to get to the muscles, which is needed for muscle activation.
Now, consider a completely opposite scenario. You’ve just eaten a large meal and you are spending
the evening watching television on your couch. You would probably feel relaxed, satisfied, and more
than a little sluggish. A different physiological response is happening, a behavior called the rest-and-
digest response. These physiological changes are driven by the other main branch of the autonomic
nervous system, called the parasympathetic nervous system.
Targets and Effects of the 2 Branches of the Autonomic

Nervous System
The sympathetic nervous system and the parasympathetic nervous system are referred to as
antagonistic. These branches are called ‘antagonistic’ because they typically have opposite effects at
target tissues.
There is dual innervation at many target organs of the autonomic nervous system. This means that
target organs receive connections from both sympathetic and parasympathetic neurons. In a sense, this
dual innervation provides both a ‘break’ and an ‘accelerator’ for changing the activity of our internal
organs, offering a good amount of control.
Both the sympathetic nervous system and parasympathetic nervous systems influence the internal
organs simultaneously. At all times, the heart is getting signals from the sympathetic nervous system
which increase heart rate, and signals from the parasympathetic nervous system which decreases heart
rate. However, this seesaw-like balance can shift quickly in either direction, such as inducing a
sympathetic response if a fearful stimulus is encountered.
Table 27.2. Comparison between sympathetic and parasympathetic action at various target organs.
Target Organ Sympathetic Action Parasympathetic Action
Eye Pupil dilation Pupil constriction
Salivary glands Prevents saliva secretion Increases saliva secretion
Lungs Airway dilation Airway constriction
Heart Increases heart rate Decreases heart rate
Blood vessels Constriction No innervation
Stomach, liver, intestines, pancreas Decreases digestion Increases digestion
Kidneys Sodium and water retention No innervation
Increases epinephrine and

Adrenal gland No innervation
norepinephrine secretion
Blood vessel dilation leading to

Reproductive organs Orgasm and ejaculation
erection
Anatomical Differences between 2 Branches of

Autonomic Nervous System
The sympathetic and parasympathetic nervous systems differ anatomically as well. The sympathetic
preganglionic neurons are short and the sympathetic postganglionic neurons are long. The
parasympathetic preganglionic neurons are long and the parasympathetic postganglionic neurons are
short.
Figure 27.7. Anatomical differences between the sympathetic and parasympathetic nervous system.
The sympathetic nervous system has short preganglionic neurons due to the proximity of the
ganglion to the spinal cord (central nervous system). The sympathetic nervous system has long
postganglionic neurons that release norepinephrine (red squares) onto target tissues. The
parasympathetic nervous system has long preganglionic neurons due to the ganglions being close
to target tissues. The postganglionic neurons of the parasympathetic nervous system are short and
release acetylcholine (blue ovals) onto target tissues. ‘Anatomical differences between sympathetic
and parasympathetic nervous systems’ by Valerie Hedges is licensed under a Creative Commons
Sympathetic Anatomy
The cell bodies of the preganglionic neurons of the sympathetic nervous system are located in the
thoracic and lumbar sections of the spinal cord, so we can use thoracolumbar to describe the site of
origin. The axons leave the central nervous system and travel only a short distance to the peripheral
ganglion. In the sympathetic system, most of the ganglia are located along the spinal cord in the
sympathetic paravertebral chain. A few sympathetic ganglia, called prevertebral, are located slightly
more laterally. The preganglionic neurons synapse on the postganglionic neurons in the sympathetic
ganglia. The postganglionic neurons then travel the rest of the distance to synapse on the target organs.
Figure 27.8. The

preganglionic
neurons of the
sympathetic
nervous system
arise from the
thoracic and
lumbar sections
of the spinal cord
and terminate in
paravertebral
and prevertebral
ganglia. The
preganglionic
neurons synapse
on
postganglionic
neurons, which
then extend to
the target organ
tissue and
innervate the
muscle and gland
cells.
‘Sympathetic
nervous system’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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One exception to this structure is the innervation of the adrenal medulla. The adrenal gland is a
structure located at the top of each kidney. The gland has an outer portion called the cortex and an
inner portion called the adrenal medulla, which is made up of cells called chromaffin cells. The
sympathetic preganglionic cell releases acetylcholine at the synapse with the chromaffin cells of the
medulla and releases acetylcholine onto the chromaffin cell that is serving as a modified post-ganglionic
neuron. Then the chromaffin cells of the adrenal medulla release epinephrine and norepinephrine
into the bloodstream. Within the bloodstream these neurotransmitters can act as hormones affecting
change throughout the body leading to a rapid onset of sympathetic activity and sustained activation.
Figure 27.9.
Autonomic
control of the
adrenal medulla.
The adrenal
glands are
located on top of
the kidneys and
consist of outer
cortex and an
inner medulla.
Sympathetic
preganglionic
neurons release
acetylcholine
onto chromaffin
cells that serve
as modified
postganglionic
neurons. The
chromaffin cells
of the adrenal
medulla release
epinephrine and
norepinephrine
into the blood
stream.
‘Sympathetic
control of the
adrenal medulla’
by Valerie
Hedges is
licensed under a
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The sympathetic preganglionic cell bodies are located in the intermediolateral cell columns in the
lateral horn of the spinal cord, a gray matter region between the dorsal and ventral horns. The neurons
send their axons out through the ventral root, and then can take one of multiple pathways. The axon
can terminate in the paravertebral ganglion at the same spinal level as the cell body, or the axon can
extend up or down the sympathetic chain to synapse on a postganglionic fiber at a different spinal level.
Another possible pathway is to enter and exit the sympathetic chain without synapsing and continue
on to terminate in a prevertebral ganglion located closer to the target organ.
Figure 27.10. The

cell bodies of the
sympathetic
preganglionic
neurons are
located in the
intermediolateral
cell column of the
spinal cord. The
preganglionic
neurons leave
the spinal cord
via the ventral
root and can
synapse in
paravertebral
ganglion at the
same spinal level
as the cell body
location or can
travel rostrally or
caudally along
the sympathetic
chain to a
different spinal
level (right side
of image).
Preganglionic
neurons can also
leave the spinal
cord, pass
through the
paravertebral
ganglia without
synapsing and
instead synapse
on a
postganglionic
neuron in a
prevertebral
ganglion (left
side of image).
‘Sympathetic
Preganglionic
Fibers’ by Casey
Henley is
licensed under a
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Commons
Attribution
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Parasympathetic Anatomy
On the other hand, parasympathetic neurons originates predominantly in the cervical spinal cord (near
the neck), with some signals originating in the sacral areas (near the tail bone). The parasympathetic
nervous system usually receives signals from several cranial nerves. Cranial nerve X, also called the
vagus nerve innervates multiple bodily organs in the midsection of the body. For the parasympathetic
nervous system, the ganglia are located very close to the target tissues. Therefore, the preganglionic
neuron must be very long to reach all the way to the ganglion to makes its first synapse. But due to the
proximity of the ganglion to the target tissue, the postganglionic neuron is very short.
Figure 27.11. The

preganglionic
neurons of the
parasympathetic
nervous system
arise from the
brainstem and
sacral sections of
the spinal cord
and terminate in
ganglia located
on or near the
target organs.
The
preganglionic
neurons synapse
on
postganglionic
neurons, which
then innervate
the muscle and
gland cells.
‘Parasympathetic
nervous system’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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License.
Neurotransmitters of the Autonomic Nervous System

We have already established that at the first synapse in the chain of autonomic neurons, all
preganglionic neurons release acetylcholine, which binds to excitatory nicotinic receptors on the
postganglionic neurons. This is true of both the sympathetic and parasympathetic nervous system.
However, the two branches of the autonomic nervous system have different effects at target tissues
because they use different neurotransmitters that produce either stimulatory or inhibitory effects at
target tissues.
The sympathetic postganglionic neurons release norepinephrine at target tissues. Norepinephrine
(and epinephrine) binds two major classes of adrenergic receptors: alpha adrenergic receptors and
beta adrenergic receptors. The effects of norepinephrine (and epinephrine) can be either excitatory or
inhibitory depending on the properties of the postsynaptic receptor.
The parasympathetic postganglionic neurons release acetylcholine at target tissues. The target
tissues express muscarinic acetylcholine receptors. The effect of the acetylcholine can be either
excitatory or inhibitory depending on the subtype of muscarinic (metabotropic) receptors. The
location of function of these transmitters and receptors are critical for clinical use, for example, when
treating high blood pressure or sexual dysfunction.
Figure 27.12.
Acetylcholine is
the primary
neurotransmitter
in both the pre-
and
postganglionic
fibers of the
parasympathetic
nervous system
and in the
preganglionic
neurons in
sympathetic
system. The
sympathetic
postganglionic
fibers release
norepinephrine
onto their
targets.
‘Autonomic
Neurotransmitter
s’ by Casey
Henley is
licensed under a
Creative
Commons
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Autonomic Control Centers in the Brain

Visceral functions are regulated through a number of autonomic reflexes, but they can be directly
controlled by higher brain areas. Within the brain stem, the medulla most directly controls autonomic
activity. The medulla receives most of its information via the vagus nerve (a mixed nerve that contains
both sensory and motor fibers). Within the medulla are a variety of control centers that regulate
functions such as cardiovascular activity, respiratory rate, vomiting, and swallowing. The pons,
another brain stem structure also serves as an autonomic control center for respiration.
In addition to brain stem structures, there are also higher order brain areas that are important for
autonomic control. The hypothalamus can directly regulate the medulla, and is also critical for the
regulation of water balance, temperature control, and hunger. The Limbic System, made up of a
number of structures including the the hippocampus and amygdala, functions in our expression of
emotion. The limbic system structures are responsible for visceral responses associated with emotional
states, including blushing, fainting, pallor, nervous cold sweats, racing heart rate, and uneasy feelings
in the stomach.
Further, the cerebral cortex and cerebellum also function in autonomic control. The cerebral cortex
has been shown to regulate lower brain structures especially in autonomic control associated with
emotion and personality. The cerebellum has connections to the medulla that are critical for the
control of autonomic functions such as sweating and nausea.
Figure 27.13.
Autonomic
control centers of
the brain. Various
higher order
brain structures
control
autonomic
nervous system
function
including the
medulla and
pons within the
brain stem, the
hypothalamus,
the limbic
system, cortex,
and cerebellum.
‘Autonomic
control centers of
the brain’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Enteric Nervous System

The internal organs that carry out digestive functions, such as the esophagus, stomach, and intestines,
are surrounded by a dense mesh of neurons that regulate their activity. Consisting of half a billion
nerve cells, this net of neurons cause the digestive tract to increase or decrease the rate of these
processes depending on the body’s demands The enteric nervous system receives signals from both the
sympathetic and parasympathetic nervous systems, and functions without our conscious knowledge.
Historically, these digestive functions have been classified as part of the autonomic nervous system,
but these responses do not share the same reflex pathway, and the enteric signals can work entirely
independent of the vagus nerve, for example.
Key Takeaways
• There are 3 main divisions of the peripheral nervous system: the somatic nervous
system, the autonomic nervous system and the enteric nervous system.
• The somatic and autonomic nervous systems different in their efferent path, how
they synapse on target tissues, the neurotransmitters that are used, and the action
of those neurotransmitters at target tissues.
• The autonomic nervous system can be subdivided into the sympathetic and
parasympathetic nervous systems.
• The sympathetic nervous system is the “fight or flight” system and the
parasympathetic nervous system is the “rest and digest” system.
• There are anatomical differences between the sympathetic and parasympathetic
nervous system.
• Several brain areas serve as autonomic control centers.
• The enteric nervous system controls the gut and receives connections from both
autonomic nervous system branches.
Test Yourself!

Attributions
Media Attributions
• Somatic Nervous System 1 Neuron Chain © Valerie Hedges is licensed under a CC BY-NC-SA
• Autonomic two neuron chain © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Synapse en passant © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• summary © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Anatomical differences between sympathetic and parasympathetic nervous system © Valerie
Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial ShareAlike) license
• anatomy of autonomic nervous system © Casey Henley is licensed under a CC BY-NC-SA
• Adrenal medulla © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Pathways © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Parasympathetic anatomy © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Neurotransmitters of the Autonomic Nervous System © Casey Henley is licensed under a CC
BY-NC-SA (Attribution NonCommercial ShareAlike) license
• Autonomic control centers in the brain © Valerie Hedges is licensed under a CC BY-NC-SA
SENSORY SYSTEMS | 333
PART V
SENSORY SYSTEMS
334 | SENSORY SYSTEMS
GENERAL PRINCIPLES OF SENSORY SYSTEMS | 335
28.
GENERAL PRINCIPLES OF SENSORY

SYSTEMS
Each sensory system is obviously quite different in the type of stimulation that it responds to and the
manner in which environmental stimuli is converted to neuronal signaling. However, there are many
principles that can be generalized across sensory systems.
Sensory transduction
Our sensory systems work by converting different types of stimuli in the environment (i.e. visible light,
sound waves, chemical molecules) into action potentials in the nervous system. This conversion is
called sensory transduction and occurs in all sensory systems.
Sensory receptors
Sensory transduction begins at the sensory receptors. Each sensory system has specialized cells that are
able to detect the environmental stimuli. Photoreceptors detect light, chemical receptors in the
tongue and nose detect odors and taste, mechanoreceptors detect touch, and hair cells detect sound.
Receptor Potentials
We have learned about postsynaptic potentials in neurons, receptor potentials are similar membrane
potential changes that happen in sensory receptors in response to a stimulus.
336 | GENERAL PRINCIPLES OF SENSORY SYSTEMS
Receptive fields
Receptive fields are easiest to understand in the visual and somatosensory systems. The receptive field
for a neuron is the region of the retina or skin where a stimulus (light or touch) will evoke a response
in the neuron. Receptive fields in the auditory system can consist of a certain frequency of sound and/
or the location of sound in space.
Receptive fields can vary in size and shape depending on the characteristics of neuron (i.e. type,
location in body, location in pathway). Receptive fields become more complex as information travels
to the brain.
Lateral Inhibition
Lateral inhibition is a process used by sensory systems to enhance the perception of signals, particularly
at edges, points, or other changes in the stimulus. It occurs because overlapping receptive fields can
inhibit each other. This inhibition enhances the perceived differences between the stimulus and the
area not stimulated.
Neural Coding
There are a number of different ways in which the nervous system encodes complex information. Two
that are common within the sensory systems are line coding and population coding.
Labeled Line Coding

In the labeled line coding of information, one cell encodes for one type of sensory quality. Pain is a
good example of this. If a pain receptor is activated, the resulting sensation will be pain, regardless of
the manner in which the receptor is stimulated. In other words, the sensory neurons are specifically
tuned to one sensory stimulus. If that receptor-cell type was dysfunctional, the sensation will not be
perceived. For example, there is a mutation that prevents sodium channels in pain receptors (but not
other cell types) from working. When this mutation occurs, the subject cannot feel pain.
GENERAL PRINCIPLES OF SENSORY SYSTEMS | 337
Population Coding
In populating coding, one cell can encode more than one sensory modality, and it is the combination
of many cells that make up the perception. An example of this is color vision. Each color photoreceptor
is most sensitive to a specific color (blue, green, or red), but a range of wavelengths can elicit changes in
firing rates in the neuron. Therefore, the responses from a population of color photoreceptors must be
combined to perceive the full spectrum of color.
Higher level processing of taste and olfaction also uses population coding – sometimes the sense of
smell is needed in addition to the sense of taste to fully perceive a flavor. Have you ever been congested
from a cold and food just doesn’t taste the same? That’s due to this combining of the senses for a full
perception.
Pathways
In general, the route sensory information takes from the periphery to the central nervous system is
similar among most of the systems. Environmental stimuli become encoded by a specialized receptor
in the periphery. Information then enters the central nervous system via the spinal cord or brainstem
and relays through the thalamus, a structure that sits deep in the forebrain. The only sensory system
that does not relay through the thalamus is the olfactory system. The thalamus then sends projections
out to the primary cortical regions for each sensory system.
Role of the Thalamus

It’s common to hear that sensory information “relays” through the thalamus on the way to the cortex
(for example, in the paragraph above). This language can give the impression that the thalamus is
only responsible for making sure the sensory signal gets from periphery to the cortex. This greatly
underestimates the thalamic role. The thalamus is known to contribute to the processing and
modification of the sensory signal.
338 | GENERAL PRINCIPLES OF SENSORY SYSTEMS
Attributions
VISUAL SYSTEM: THE EYE | 339
29.
VISUAL SYSTEM: THE EYE
Humans are remarkably dependent on the visual system

to gain information about our surroundings. Consider
how tentatively you walk from the light switch to your Resources
bed right after turning off the lights!
The visual system is complex and consists of several
interacting anatomical structures. Here, we will describe
• Glossary Terms
the process of how photons of light from our
• Key Takeaways
surroundings become signals that the brain turns into
• Test Yourself
representations of our surroundings.
• Additional Review
Properties of Light
Visual sensation starts at the level of the eye. The eye is an organ that has evolved to capture photons,
the elementary particle of light. Photons are unusual because they behave as both particles and as
waves, but neuroscientists mostly focus on the wave-like properties. Because photons travel as waves,
they oscillate at different frequencies. The frequency at which a photon oscillates is directly related to
the color that we perceive.
The human visual system is capable of seeing light in a very narrow range of frequencies on the
electromagnetic spectrum. On the short end, 400 nm wavelengths are observed as violet, while on
the long end, 700 nm wavelengths are red. Ultraviolet light oscillates at a wavelength shorter than 400
nm, while infrared light oscillates at a wavelength longer than 700 nm. Neither ultraviolet nor infrared
light can be detected with our eyes.
340 | VISUAL SYSTEM: THE EYE
Figure 29.1.
Electromagnetic
spectrum.
Humans are only
able to perceive
wavelengths of
light between
400nm and
700nm. The
wavelength is
directly related to
the perceived
color that is seen.
Shorter
wavelengths are
perceived as
violet, where are
longer
wavelengths are
perceived as red.
Anatomy of the Eye

Photons pass through several anatomical structures before the nervous system processes and interprets
them. The front of the eye consists of the cornea, pupil, iris, and lens. The cornea is the transparent,
external part of the eye. The cornea refracts, or bends, the incoming rays of light so that they converge
precisely at the retina, the posterior most part of the eye. If the light rays fail to properly converge, a
person would be near-sighted or far-sighted, and this would result in blurry vision. Glasses or contact
lenses bend light before it reaches the cornea to compensate the cornea’s shape.
After passing through the cornea, light enters through a hole in the opening in the iris at the center
of the eye called the pupil. The iris is the colored portion of the eye that surrounds the pupil and along
with local muscles that can control the size of the pupil to allow for an appropriate amount of light
to enter the eye. The diameter of the pupil can change depending on ambient light conditions. In the
dark, the pupil dilates, or gets bigger, which allows the eye to capture more light. In bright conditions,
the pupils constricts, or gets smaller, which decreases the amount of light that enters the eye.
The next structure that light passes through is the lens. The lens is located behind the pupil and iris.
Like the cornea, the lens refracts light so that the rays converge on the retina. Proper focusing requires
the lens to stretch or relax, a process called accommodation. A circular muscle that surrounds the lens,
called the ciliary muscle, changes the shape of the lens depending on the distance of the object of
focus.
The retina is the light-sensitive region in the back of the eye where the photoreceptors, the
specialized cells that respond to light, are located. The retina covers the entire back portion of the eye,
so it’s shaped like a bowl. In the middle of the bowl is the fovea, the region of highest visual acuity,
meaning the area that can form the sharpest images. The optic nerve projects to the brain from the
back of the eye, carrying information from the retinal cells. Where the optic nerve leaves, there are no
photoreceptors since the axons from the neurons are coming together. This region is called the optic
disc and is the location of the blind spot in our visual field.
Figure 29.2. Cross section of the eye. The visible regions of the eye include the cornea, pupil (gray
region), and iris (blue region). The lens sits behind the pupil and iris. The retina (red line) is located
along the back of the eye. The fovea (dark red section) is a small portion of the retina where visual
acuity is highest, and the optic disc is located where the optic nerve (tan region) leaves the eye.
Details about the functions of each region are in the text. ‘Eye Anatomy’ by Casey Henley is licensed
Retinal Cells
In addition to the photoreceptors, there are four other cell types in the retina. The photoreceptors
synapse on bipolar cells, and the bipolar cells synapse on the ganglion cells. Horizontal and amacrine
cells allow for communication laterally between the neuron layers.
Figure 29.3. There are five cell types in the retina. The photoreceptors synapse on bipolar cells, and
the bipolar cells synapse on ganglion cells. The horizonal cells allow for communication between
photoreceptors by interacting with the photoreceptor-bipolar cell synapse, and the amacrine cells
allow for communication between bipolar cells by interacting at the bipolar cell-ganglion cell
synapse. ‘Retinal Neurons’ by Casey Henley is licensed under a Creative Commons Attribution
Direction of Information
When light enters the eye and strikes the retina, it must pass through all the neuronal cell layers
before reaching and activating the photoreceptors. The photoreceptors then initiate the synaptic
communication back toward the ganglion cells.
Figure 29.4. When light enters the eye, it must pass through the ganglion and bipolar cell layers
before reaching the photoreceptors. The neuronal communication travels in the opposite direction
from the photoreceptors toward the ganglion cells. ‘Light in the Retina’ by Casey Henley is licensed
Photoreceptors
Photoreceptors are the first cells in the neuronal visual perception pathway. The photoreceptors are the
specialized receptors that respond to light. They are the cells that detect photons of light and convert
them into neurotransmitter release, a process called phototransduction.
Morphologically, photoreceptor cells have two parts, an outer segment and inner segment. The
outer segment contains stacks of membranous disks bounded within the neuronal membrane. These
membranous disks contain molecules called photopigments, which are the light-sensing components
of the photoreceptors. Hundreds of billions of these photopigments can be found in a single
photoreceptor cell. The inner segment contains the nucleus and other organelles. Extending from the
inner segment is the axon terminal.
Photoreceptors are classified into two categories, named because of their appearance and shape: rods
and cones. Rod photoreceptors have a long cylindrical outer segment that holds many membranous
disks. The presence of more membranous disks means that rod photoreceptors contain more
photopigments and thus are capable of greater light sensitivity.
Cone photoreceptors have a short, tapered c, and cylindrical outer segment that holds fewer
membranous disks than rod photoreceptors. The presence of less membranous disks means that cone
photoreceptors contain less photopigments and thus are not as sensitive to light as rod photoreceptors.
Cone photoreceptors are responsible for processing our sensation of color (the easiest way to remember
this is cones = color). The typical human has three different types of cone photoreceptors cells, with
each of these three types tuned to specific wavelengths of light. The short wavelength cones (S-cones)
respond most robustly to 420 nm violet light. The middle wavelength cones (M-cones) exhibit peak
responding at 530 nm green light, and the long wavelength cones (L-cones) are most responsive in 560
nm red light. Each of these cones is activated by other wavelengths of light too, but to a lesser degree.
Every color on the visible spectrum is represented by some combination of activity of these three cone
photoreceptors.
The idea that we have two different cellular populations and circuits that are used in visual
perception is called the duplicity theory of vision and is our current understanding of how the
visual system perceives light. It suggests that both the rods and cones are used simultaneously and
complement each other. The photopic vision, uses cone photoreceptors of the retina, and is
responsible for high-acuity sight and color vision in daytime. Its counterpart, called scotopic vision,
uses rod photoreceptors and is best for seeing in low-light conditions, such as at night. Both rods and
cones are used for mesopic vision, when there are intermediate lighting conditions, such as indoor
lighting or outdoor traffic lighting at night.
Figure 29.5. The rods and cones have different physical appearances and play separate roles in
visual processing. ‘Rod and Cone’ by Casey Henley is licensed under a Creative Commons Attribution
Photoreceptor Density
In addition to having different visual functions, the rods and cones are also distributed across the retina
in different densities. Visual information from our peripheral vision is generally detected by our rod
cells, which are most densely concentrated outside the fovea. Cone photoreceptor cells allow for high-
acuity vision. They are most densely packed at the fovea, corresponding to the very center of your visual
field. Despite being the cell population that we use for our best vision, cone cells make up the minority
of photoreceptors in the human retina, outnumbered by about 20-times more rod cells.
Figure 29.6.
Graph depicting
the number of
cells in (mm2)
across the retina.
Cones are
concentrated at
the center of the
retina (0
degrees) in the
fovea. There are
many more rods
than there are
cones. The rods
are located in the
periphery, but
not at the center
of the retina.
There are neither
cones nor rods at
the blind spot,
which is where
the retinal
ganglion cells exit
the retina.
Synaptic Convergence of Photoreceptors

Rod cells are organized to have high synaptic convergence, where several rod cells (up to 30) feed into
a single downstream route of communication (the bipolar cells, to be specific). An advantage of a high-
convergence network is the ability to add many small signals together to create a seemingly larger signal.
Consider stargazing at night, for example. Each rod is able to detect low levels of light, but signals from
multiple rod cells, when summed together, allows you to recognize faint light sources such as a star. A
disadvantage of this type of organization is that it is difficult to identify exactly which photoreceptor
is activated by the incoming light, which is why accuracy is poor when seeing stimuli in our peripheral
vision. This is one of the reasons that we cannot actually read text in our peripheral vision or see the
distinct edges of a star. Rod photoreceptors are maximally active in low-light conditions.
Unlike rod cells, cone cells have very low synaptic convergence. In fact, at the point of highest visual
acuity, a single cone photoreceptor communicates with a single pathway to the brain. The signaling
from low-convergence networks is not additive, so they are less effective at low light conditions.
However, because of this low-convergence organization, cone cells are highly effective at precisely
identifying the location of incoming light.
Figure 29.7.
Photoreceptor
Convergence.
Cones have very
low synaptic
convergence,
meaning that
single cone
photoreceptors
ultimately signal
to a single retinal
ganglion cell,
providing high
visual acuity in
cones. Rods,
however, have
high synaptic
convergence,
meaning many
rod
photoreceptors
ultimately signal
to a single retinal
ganglion cell. This
gives rods the
ability to better
see objects in
low light.
Retina: Fovea and Optic Disk

The retina is not completely uniform across the entire back of the eye. There are a few spots of
particular interest along the retina where the cellular morphology is different: the fovea and the optic
disk.
There are two cellular differences that explain why the fovea is the site of our best visual acuity.
For one, the neurons found at the fovea are “swept” away from the center, which explains why the
fovea looks like a pit. Cell membranes are made up mostly of lipids, which distort the passage of
light. Because there are fewer cell bodies present here, the photons of light that reach the fovea are
not refracted by the presence of other neurons. Secondly, the distribution of photoreceptors at the
fovea heavily leans toward cone type photoreceptors. Because the cone cells at the fovea exhibit low
convergence, they are most accurately able to pinpoint the exact location of incoming light. On the
other hand, most of the photoreceptors in the periphery are rod cells. With their high-convergence
circuitry, the periphery of the retina is suited for detecting small amounts of light, though location and
detail information is reduced.
Figure 29.8.
Fovea. The fovea
is a pit at the
center of the
retina and
provides the
most visual
acuity. This is due
to the cells of the
retina
(photoreceptors,
bipolar cells,
retinal ganglion
cells) being
pushed to the
side of the fovea,
leaving no cells
to distort the
light as it reaches
the
photoreceptors.
‘Fovea’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Another anatomically interesting area of the retina is an elliptical spot called the optic disk. This is
where the optic nerve exits the eye. At this part of the retina, there is an absence of photoreceptor cells.
Because of this, we are unable to perceive light that falls onto the optic disk. This spot in our vision is
called the blind spot.
Figure 29.9. Rods and cones are distributed across the retina in different densities. Cones are located
at the fovea. Rods are located everywhere else. The optic disc lacks all photoreceptors since the
optic nerve fibers are exiting the eye at this location. ‘Retinal Receptor Density’ by Casey Henley is
Phototransduction
The photoreceptors are responsible for sensory transduction in the visual system, converting light into
electrical signals in the neurons. For our purposes, to examine the function of the photoreceptors, we
will A) focus on black and white light (not color vision) and B) assume the cells are moving from either
an area of dark to an area of light or vice versa.
Photoreceptors do not fire action potentials; they respond to light changes with graded receptor
potentials (depolarization or hyperpolarization). Despite this, the photoreceptors still release
glutamate onto the bipolar cells. The amount of glutamate released changes along with the membrane
potential, so a hyperpolarization will lead to less glutamate being released. Photoreceptors

hyperpolarize in light and depolarize in dark. In the graphs used in this lesson, the starting membrane
potential will depend on the initial lighting condition.
Figure 29.10. Photoreceptors respond with graded potentials when moving from light to dark or vice
versa. A) When moving from dark to light, the photoreceptor will hyperpolarize, and glutamate
release will decrease. B) When moving from light to dark, the photoreceptor will depolarize, and
glutamate release will increase. ‘Photoreceptor Receptor Potentials’ by Casey Henley is licensed
When the photoreceptor moves into the light, the cell hyperpolarizes. Light enters the eye, reaches the
photoreceptors, and causes a conformational change in a special receptor protein called an opsin. The
opsin receptor has a pre-bound chemical agonist called retinal. Together, the opsin + retinal makes up
the photopigment rhodopsin.
When rhodopsin absorbs light, it causes a conformational change in the pre-bound retinal, in
a process called “bleaching”. The bleaching of rhodopsin activates an associated G-protein called
transducin, which then activates an effector enzyme called phosphodiesterase (PDE). PDE breaks
down cGMP in the cell to GMP. As a result, the cGMP-gated ion channels close. The decrease in
cation flow into the cell causes the photoreceptor to hyperpolarize.
Animation 29.1. Light reaching the photoreceptor causes a conformational change in the opsin
protein, which activates the G-protein transducing. Transducin activates phosphodiesterase (PDE),
which converts cGMP to GMP. Without cGMP, the cation channels close, stopping the influx of
positive ions. This results in a hyperpolarization of the cell. ‘Phototransduction’ by Casey Henley is
In the dark, the photoreceptor has a membrane potential that is more depolarized than the “typical”
neuron we examined in previous chapters; the photoreceptor membrane potential is approximately -40
mV.
Rhodopsin is not bleached, thus the associated G-protein, transducin, remains inactive. As a result,
there is no activation of the PDE enzyme, and levels of cGMP within the cell remain high. cGMP binds
to cGMP-gated sodium ion channels, causing them to open. The open cation channels allow the influx
of sodium and calcium, which depolarize the cell in the dark.
Figure 29.11. In the dark, the photoreceptor is depolarized due to an influx of sodium and calcium
through open ion channels that are gated by cGMP. The photoreceptor has high levels of cGMP
when it is in the dark. Additionally, the opsin proteins, the G-protein transducin, and
phosphodiesterase (PDE) are all inactivated. ‘Retinal Dark Current’ by Casey Henley is licensed
Transmission of Information within Retina

Photoreceptors synapse onto bipolar cells in the retina. There are two types of bipolar cells: OFF-
center bipolar cells and ON-center bipolar cells. These cells respond in opposite ways to the glutamate
released by the photoreceptors because they express different types of glutamate receptors. Like
photoreceptors, the bipolar cells do not fire action potential and only respond with graded
postsynaptic potentials.
OFF-center Bipolar Cells

In OFF-center bipolar cells, the glutamate released by the photoreceptor is excitatory. OFF-center
bipolar cells express ionotropic glutamate receptors. In the dark, the photoreceptor is depolarized, and
thus releases more glutamate. The glutamate released by the photoreceptor activates the ionotropic
receptors, and sodium can flow into the cell, depolarizing the membrane potential. In the light, the
photoreceptor is hyperpolarized, and thus does not release glutamate. This lack of glutamate causes the
ionotropic receptors to close, preventing sodium influx, hyperpolarizing the membrane potential of
the OFF-center bipolar cell. One way to remember this is that OFF-center bipolar cells are excited by
the dark (when the lights are OFF).
Figure 29.12. Photoreceptors hyperpolarize in light and decrease the amount of released glutamate.
Glutamate is excitatory in OFF bipolar cells, opening ionotropic receptors and allowing sodium
influx. In the dark, the OFF bipolar cells are depolarized, and in the light the OFF bipolar cells are
hyperpolarized. ‘Off Bipolar Cells’ by Casey Henley is licensed under a Creative Commons Attribution
ON-center Bipolar Cells

In ON-center bipolar cells, the glutamate released by the photoreceptor is inhibitory. ON-center
bipolar cells express metabotropic glutamate receptors. In the dark, the photoreceptor is depolarized,
and thus releases more glutamate. The glutamate released by the photoreceptor binds to the
metabotropic receptors on ON-center bipolar cells, and the G-proteins close cation channels in the
membrane, stopping the influx of sodium and calcium, hyperpolarizing the membrane potential.
In the light, the photoreceptor is hyperpolarized, and thus does not release glutamate. The absence
of glutamate results in the ion channels being open and allowing cation influx, depolarizing the
membrane potential. You can remember that ON-center bipolar cells are excited by the light (when the
lights are ON).
Figure 29.13. Photoreceptors hyperpolarize in light and decrease the amount of released glutamate.
Glutamate is inhibitory in ON bipolar cells, activating metabotropic receptors, which closes cation
channels. In the dark, the ON bipolar cells are hyperpolarized, and in the light the ON bipolar cells
are depolarized. ‘ON Bipolar Cells’ by Casey Henley is licensed under a Creative Commons
Retinal Ganglion Cells

Retinal ganglion cells are the third and last cell type that directly conveys visual sensory information,
receiving inputs from the bipolar cells. OFF-center and ON-center bipolar cells synapse on OFF-center
and ON-center ganglion cells, respectively. The axons of the retinal ganglion cells bundle together and
form the optic nerve, which then exits the eye through the optic disk. Retinal ganglion cells are the
only cell type to send information out of the retina, and they are also the only cell that fires action
potentials. The ganglion cells fire in all lighting conditions, but it is the relative firing rate that encodes
information about light. A move from dark to light will cause OFF-center ganglion cells to decrease
their firing rate and ON-center ganglion cells to increase their firing rate.
Figure 29.14. A move from dark to light will hyperpolarize all photoreceptors. OFF bipolar cells will
also hyperpolarize in light, which will lead to a decreased firing rate in OFF-center ganglion cells. ON
bipolar cells will depolarize in light, which will lead to an increased firing rate in ON-center ganglion
cells. ‘Retinal Ganglion Cells’ by Casey Henley is licensed under a Creative Commons Attribution
Receptive Fields
Each bipolar and ganglion cell responds to light stimulus in a specific area of the retina. This region of
retina is the cell’s receptive field. Receptive fields in the retina are circular.
Size of the receptive field can vary. The fovea has smaller receptive fields than the peripheral retina.
The size depends on the number of photoreceptors that synapse on a given bipolar cell and the number
of bipolar cells that synapse on a given ganglion cell, also called the amount of convergence.
Figure 29.15. Ganglion receptive field sizes can vary depending on location of the bipolar and
ganglion cells and the amount of convergence onto those cells. When the photoreceptors are in or
near the fovea (Cell 1), the receptive fields are small. In the fovea, each bipolar cell receives input
from only one photoreceptor and then synapses on only one ganglion cell. Toward the periphery
(Cells 2 and 3), more photoreceptors synapse on each bipolar cell, and more bipolar cells synapse on
each ganglion cell, making the surface area of the receptive field larger. ‘Retinal Receptive Field’ by
Receptive Field Example

Let’s use an example of an ON-center bipolar cell to look at the structure of receptive fields
in the retina. The bipolar and retinal ganglion cell receptive fields are divided into two
regions: the center and the surround. The center of the receptive field is a result of direct
innervation between the photoreceptors, bipolar cells, and ganglion cells. If a light spot
covers the center of the receptive field, the ON-center bipolar cell would depolarize, as
discussed above; the light hits the photoreceptor, it hyperpolarizes, decreasing glutamate
release. Less glutamate leads to less inhibition of the ON bipolar cell, and it depolarizes.
Figure 29.16. A photoreceptor in the center of an ON bipolar cell’s receptive field moves from dark to
light. The photoreceptor will hyperpolarize, and the ON bipolar cell will depolarize. The red arrows
show the direct synaptic communication from photoreceptor to ON bipolar cell. ‘Light in Center’ by
The surround portion of the receptive field is a result of indirect communication among the
retinal neurons via horizontal and amacrine cells. The surround has an opposing effect on
the bipolar or ganglion cell compared to the effect of the center region. That is to say, that
the center and surround of the receptive field are opposite to each other. So, an ON-center
bipolar or ganglion cell, can also be referred to as an “ON-center OFF-surround cell”, and an
OFF-center bipolar or ganglion cell can also be referred to as an “OFF-center ON-surround
cell”.
Therefore, if light covers the surround portion, the ON-center bipolar cell would respond by
hyperpolarizing. The light would cause the photoreceptor in the surround to hyperpolarize.
This would cause the horizontal cell to also hyperpolarize. Horizontal cells have inhibitory
synaptic effects, so a hyperpolarization in the horizontal cell would lead to a depolarization
in the center photoreceptor. The center photoreceptor would then cause a
hyperpolarization in the ON-center bipolar cell. These effects mimic those seen when the
center is in dark. So, even though the center photoreceptor is not directly experiencing a
change in lighting conditions, the neurons respond as if they were moving toward dark.
Figure 29.17. A photoreceptor in the surround of an ON bipolar cell’s receptive field moves from dark to
light. The photoreceptor will hyperpolarize, and the postsynaptic horizontal cell will hyperpolarize.
This will cause the center photoreceptor to depolarize, and the ON bipolar cell to hyperpolarize. The
red arrows show the indirect synaptic communication between the surround photoreceptor and the
ON bipolar cell. The surround photoreceptor synapses on the horizontal cell, which synapses on the
center photoreceptor, which synapses on the bipolar cell. ‘Light in Surround’ by Casey Henley is
Lateral Inhibition
The center-surround structure of the receptive field is critical for lateral inhibition to occur. Lateral
inhibition is the ability of the sensory systems to enhance the perception of edges of stimuli. It is
important to note that the photoreceptors that are in the surround of one bipolar cell would also be in
the center of a different bipolar cell. This leads to a direct synaptic effect on one bipolar cell while also
having an indirect effect on another bipolar cell.
Figure 29.18. An edge of a light stimulus moves into the receptive field surround of ON bipolar cell B.
This edge is also falling on the receptive field center of ON bipolar cell C. The light will cause bipolar
cell C to depolarize because of the direct synapse with the photoreceptor. The light will also cause
bipolar cell B to hyperpolarize because of the indirect synapses through the horizontal cell. This
hyperpolarization causes a larger membrane potential difference between cells B and C that would
occur if the horizontal cells were absent. The larger membrane potential difference between the
cells will lead to an enhancement in the perception between the dark and light side of the edge.
‘Lateral Inhibition’ by Casey Henley is licensed under a Creative Commons Attribution
Although some of the images used here will simplify the receptive field to one cell in the center and
a couple in the surround, it is important to remember that photoreceptors cover the entire surface of
the retina, and the receptive field is two-dimensional. Depending on the level of convergence on the
bipolar and ganglion cells, receptive fields can contain many photoreceptors.
Figure 29.19. The receptive fields exist in two-dimensions along the surface of the retina. Depending
on the location of the receptive field, and the amount of convergence that occurs at the bipolar or
ganglion cell, the receptive field may contain many photoreceptors. ‘Retinal surface’ by Casey
Key Takeaways
• Photoreceptors and bipolar cells do not fire action potentials

• Photoreceptors hyperpolarize in the light
• ON bipolar cells express inhibitory metabotropic glutamate receptors
• OFF bipolar cells express excitatory ionotropic glutamate receptors
• Receptive fields are circular, have a center and a surround, and vary in size
• Receptive field structure allows for lateral inhibition to occur
Test Yourself!
Additional Review
1. Compare and contrast rods and cones.

2. Compare and contrast the fovea and the optic disc.
Answers
Attributions
Media Attributions
• EM_spectrumrevised © Philip Ronan, Gringer is licensed under a CC BY-NC-SA (Attribution

• Distribution_of_Cones_and_Rods_on_Human_Retina © Jochen Burghardt is licensed under
a CC BY-NC-SA (Attribution NonCommercial ShareAlike) license
• Convergence © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Fovea
VISUAL SYSTEM: CENTRAL PROCESSING | 363
30.
VISUAL SYSTEM: CENTRAL

PROCESSING
In this chapter we will learn about how the information

from the retina is processed centrally within the brain.
Resources
Visual Fields
• Glossary Terms
Before learning the pathway that visual information takes
• Scientist links to learn
from the retina to the cortex, it is necessary to understand
more
how the retina views the world around us. The full visual
• Key Takeaways
field includes everything we can see without moving our
• Test Yourself
head or eyes.
364 | VISUAL SYSTEM: CENTRAL PROCESSING
Figure 30.1. The two eyes together can view the entire visual field, which is all the visual space we
can see without moving our head or eyes. ‘Full Visual Field’ by Casey Henley is licensed under a
License.
The full visual field can be divided in a few ways. Each individual eye is capable of seeing a portion of,
but not the entire, visual field.
Figure 30.2. Each eye individually can view only a portion of the full visual field. ‘Single Eye Fields’ by
The full visual field can also be divided into the right and left hemifields. The hemifields range from
the most peripheral point to the center point, splitting the full visual field into two equal regions. Both
eyes are involved in viewing each hemifield. The fovea separates the retina into two sections: the nasal
retina and the temporal retina. The nasal retina is the medial portion that is located toward the nose.
The temporal retina is the lateral portion that is located toward the temples and temporal lobe. The
nasal retina from one eye along with the temporal retina from the other eye are able to view an entire
hemifield.
Figure 30.3. The full visual field can be divided into left and right hemifields. Both eyes contribute to
viewing these regions. The nasal retina of the left eye and the temporal retina of the right eye view
the left hemifield. The nasal retina of the right eye and the temporal retina of the left eye view the
right hemifield. ‘Visual Hemifields’ by Casey Henley is licensed under a Creative Commons
Finally, the full visual field can be separated into monocular and binocular regions. Each monocular
field is visual space that can only be viewed by one eye. The binocular region is visual space that can be
viewed by both eyes.
Figure 30.4. Monocular visual fields are viewed by only one eye and are located toward the
periphery of the full visual field. The binocular visual field is viewed by both eyes and is located in
the center of the full visual field. ‘Monocular and Binocular Fields’ by Casey Henley is licensed under
License.
Pathway to Brain
Visual information from each eye leaves the retina via the ganglion cell axons at the optic disc, creating
the optic nerve. The optic nerve, or cranial nerve II, exits the posterior end of the eyeball, and travels
posteriorly along the ventral surface of the brain. Like all other cranial nerves, the optic nerve is paired,
meaning there is one for each eye. Both optic nerves merge at a spot called the optic chiasm, then
diverge yet again as they travel posteriorly towards the thalamus.
The axonal connections in the optic nerve are not quite as simple as its anatomical appearance.
From each optic nerve, some of the nerve fibers cross the midline (decussate), headed towards the
contralateral hemisphere. Other nerve fibers meet at the optic chiasm, but then project into the
ipsilateral hemisphere.
Prior to entering the brain, axons from the nasal portion of each retina cross the midline at the optic
chiasm but the temporal portion of each retina does not cross at the optic chiasm.
Figure 30.5. Information from each eye is carried away from the retina by the optic nerve.
Information perceived by neurons in the nasal retina of each eye crosses the midline at the optic
chiasm. Information from the contralateral visual hemifield then travels to the brain. ‘Pathway from
Retina’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
View the optic nerve (cranial nerve II) using the BrainFacts.org 3D Brain
Since the axons from the nasal retina cross to the opposite side of the nervous system but the
temporal retina axons do not, this leads to the brain processing input from the contralateral (opposite
side) visual hemifield. Therefore, the right side of the brain receives visual information from the left
hemifield and vice versa. The easy way to keep track of this unusual system is to remember that all
information from the left visual field enters the right hemisphere of the brain, while visual information
from the right visual field enters the left hemisphere of the brain.
Figure 30.6
Pathway from
the retina to the
brain.
Information
collected from
the left visual
hemifield (shown
in blue) comes by
way of the left
nasal retina and
right temporal
retina. This left
visual hemifield
information is
processed within
the right lateral
geniculate
nucleus and then
the right primary
visual cortex.
Information
collected from
the right visual
hemifield (shown
in red) comes by
way of the right
nasal retina and
the left temporal
retina. The right
visual hemifield
information is
processed within
the left lateral
geniculate
nucleus and then
the left primary
visual cortex.
Pathways from the Retina

The retinal ganglion cells that exit the retina and project into the brain can take different paths. The
retinofugal projection (fugal means “to flee”) is one of the major projection paths of the retinal
ganglion cells. Most retinal output projects to the lateral geniculate nucleus of the thalamus and
then to the primary visual cortex, however there are subsets of cells that are routed through other
non-thalamic pathways.
Non-Thalamic Pathways
Not all of the axons convey direct visual information into the thalamus for visual perception. Some
ganglion cells project to the superior colliculus, a midbrain region via the retinotectal pathway
(recall that the superior colliculus is a structure of the tectum). This pathway communicates with
motor nuclei and is responsible for pupillary control. Specifically, it is responsible for movements that
will orient the head and eyes toward an object to focus the object in the center of the visual field, the
region of highest visual acuity.
A subset of specialized retinal ganglion cells project to the suprachiasmatic nucleus in the
hypothalamus through the retinohypothalamic tract (starts in the retina, ends in the
hypothalamus). It does not carry any conscious visual information. The retinohypothalamic tract
conducts light information from a small group of intrinsically-photosensitive retinal ganglion cells.
This structure functions to help the body adapt its sleep-wake cycle in the face of changing day-night
patterns This region is critical for circadian rhythms and the sleep/wake cycle.
Figure 30.7. In addition to the thalamus, the retinal neurons send projections to other regions of the
brain. The suprachiasmatic nucleus (pink) is located in the hypothalamus and is important for
biological rhythms. The pretectum (light blue) is a midbrain structure that plays a role in muscle
control of the pupil. Finally, the retina projects to the superior colliculus (blue), another midbrain
region important in eye and head movements. The lateral geniculate nucleus of the thalamus
(green) is also shown. ‘Non-Thalamic Retinal Pathways’ by Casey Henley is licensed under a
License.
View the hypothalamus using the BrainFacts.org 3D Brain

View the midbrain using the BrainFacts.org 3D Brain
Thalamic Pathway: Lateral Geniculate Nucleus

In conscious visual perception, the first synapse of the optic nerve is formed in the thalamus at a
subregion called the lateral geniculate nucleus, or LGN. The optic tract enters the brain and ascends
to synapse in the lateral geniculate nucleus of the thalamus. From there, axons project to the primary
visual cortex, also called the striate cortex or V1, located in the occipital lobe.
Figure 30.8. A horizontal section of the brain. The optic tract enters the brain and projects dorsally
to the thalamus. Information is then sent to the primary visual cortex in the occipital lobe. ‘CNS
Visual Pathway’ by Casey Henley is licensed under a Creative Commons Attribution
Lateral Geniculate Nucleus

The lateral geniculate nucleus of the thalamus (LGN) has six distinct layers when examined in cross
section. The LGN serves as the first synaptic site of the retinal ganglion cells that exited the retina.
Importantly, visual information is separated at the level of the LGN such that visual information
from each visual field is all processed in the contralateral LGN. This means that information from the
left visual hemifield, collected via the right temporal retina and the left nasal retina will all be processed
within the right LGN. Information from the right visual hemifield, collected via the left temporal
retina and the right nasal retina will all be processed by the left LGN.
Within the LGN, input coming from each eye is kept separate by the layers of the LGN. For
example, notice that half of the layers of the right LGN process information from the right eye and the
other half of the layers process information from the left eye.
Figure 30.9.
Lateral
Geniculate
Nucleus. The
Lateral
Geniculate
Nucleus (LGN)
has six layers in
cross section.
The visual
information from
the left visual
hemifield, coming
from the left
nasal retina and
the right
temporal retina,
is all processed
by the right
lateral geniculate
nucleus. The
visual
information from
the right visual
hemifield, coming
from the right
nasal retina and
the left temporal
retina, is all
processed by the
left lateral
geniculate
nucleus. Within
an individual
lateral geniculate
nucleus, the
information from
each eye is kept
in separate
layers.
Information from
the nasal retinas
crosses and is
processed by the
contralateral
lateral geniculate
nucleus, whereas
information from
the temporal
retinas is
processed by the
ipsilateral lateral
geniculate
nucleus (does not
cross). ‘Lateral
Geniculate
Nucleus’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
The outputs of the LGN are a series of axonal bundles called the optic radiations. From the LGN
of the thalamus, the optic radiations project to the occipital lobe at the caudal (posterior) end of the
brain. Once visual information travels into the cortex, the process is less about sensation and mostly
about perception.
Figure 30.10.
Pathway from
the retina to the
brain.
Information
collected from
the left visual
hemifield (shown
in blue) comes by
way of the left
nasal retina and
right temporal
retina. This left
visual hemifield
information is
processed within
the right lateral
geniculate
nucleus and then
the right primary
visual cortex.
Information
collected from
the right visual
hemifield (shown
in red) comes by
way of the right
nasal retina and
the left temporal
retina. The right
visual hemifield
information is
processed within
the left lateral
geniculate
nucleus and then
the left primary
visual cortex.
Information from
the LGN travels
to the primary
visual cortex via
the optic
radiations.
Figure 30.11.
Visual
information that
is sent through
the full visual
pathway,
therefore, moves
from
photoreceptor to
bipolar cell to
ganglion cell in
the retina. It
leaves the retina
via the optic
nerve, optic
chiasm, and optic
tract to the
lateral geniculate
nucleus of the
thalamus and
then travels to
the primary
visual cortex via
the optic
radiation. ‘Visual
Pathway’ by
Casey Henley is
licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
View the thalamus using the BrainFacts.org 3D Brain
Striate Cortex (Primary Visual Cortex)

The outputs of the LGN are axons which form synapses in the primary visual cortex, which is also
called V1 or the striate cortex. ‘Striate’ means ‘stripe’ and is named due to the presence of a large
white stripe that can be seen in unstained tissue during surgical dissection. This white stripe is the
bundle of incoming optic tract axons, which are heavily myelinated.
Each neuron in V1 receives visual information from a specific patch of retinal cells. This
organizational pattern, where a section of retinal inputs map onto neurons of a specific section of
V1, is called retinotopic organization. This retinotopic organization is conserved from the retina to
the LGN, and finally to the primary visual cortex. Visual information from the fovea, despite being
only 1% of the total visual field, takes up about half of all neurons in V1. After processing in V1,
visual information is passed along to other cortical areas that contribute to various aspects of visual
perception.
View the primary visual cortex using the BrainFacts.org 3D Brain
Figure 30.12.
Primary Visual
Cortex. The
primary visual
cortex (also
called striate
cortex or V1) is
located in the
posterior region
of the human
brain within the
occipital lobes. In
this image the
primary visual
cortex is marked
with V1 and
highlighted in
green.
Cortical Layers
The cortex of the brain is arranged into six layers, named with the Roman numerals I-VI. The cells
within these layers have different morphology. These cortical layers are especially visible within the
striate cortex (V1). Within this region, the thickness of the cortex between the pia mater that is in
contact with the top of the cortex and the white matter underlying the cortex is very thin (around 2
mm in thickness).
Figure 30.13.
Laminar
organization of
the cortex. When
looking at a cross
section of the
cortex, six
distinct layers of
cells can be
observed. These
layers are
typically labeled
by the Roman
numerals I-VI.
Cells within each
of the layers
have distinct
morphology.
These cells within the six layers of the cortex are arranged in what are called ‘cortical columns’. This
arrangement was discovered by Dr. Vernon Mountcastle in the 1950s. You can imagine that due to the
directionality of communication in neurons, that information travels up and down the cells within a
single cortical column.
Figure 30.14.
Columnar
organization of
the cerebral
cortex. The
cerebral cortex
has cells that are
organized into
columns, where
all the cells
within a single
cortical column
communicate
with each other.
Features of the Primary Visual Cortex:

Contributions of David Hubel and Torsten Wiesel

Dr. David Hubel and Dr. Torsten Wiesel followed up on this critical discovery by Mountcastle to
describe neuronal processing within the visual cortex. Their discoveries in this field led to them being
awarded the Nobel Prize in the field of Physiology or Medicine in 1981. Highlighted below are some
of their major contributions.
Orientation Selectivity
Hubel and Wiesel used microelectrodes to map the functioning of the visual cortex. In one of their
famous experiments, Hubel and Wiesel used anesthetized cats to determine how the visual cortex
processes visual information. During their experiment, they were showing the anesthetized cat
different visual stimuli through the projection of slides with different images onto a screen while
recording from neurons within the visual cortex. Due to when this experiment was done, these slides
were large and plastic and had to be physically inserted into a projector. In the process of inserting these
slides into the projector, a bar of light was projected onto the screen by the edge of the slide. Quite by
surprise, the act of changing the slides (and the bar of light that resulted) caused the neurons within the
cat brain to fire action potentials. Hubel and Wiesel determined that the stimulus causing the neurons
to fire was the angle of the bar of light projected on the screen.
In fact, the neurons within the visual cortex responded best to a line in a specific orientation and
the firing rate of the neuron increased as the line rotated toward the “preferred” orientation. The firing
rate is highest when the line is in the exact preferred orientation and different orientations are preferred
by different neurons. This discovery was due to some great luck for Hubel and Wiesel as they were
recording from neurons that had a preferred orientation that exactly matched the angle of changing
the slides in their projector!
Figure 30.15.
Orientation
selectivity
experiment. In
Hubel and
Wiesel’s
experiment they
recorded from
neurons within
the primary
visual cortex of
anesthetized cats
while showing
them images on
slides projected
onto a screen
(left). They found
that neurons
within the
primary visual
cortex fired
action potentials
in response to a
bar of light in a
specific
orientation
(horizontal in the
image above).
The most action
potentials are
fired when the
bar of light is in
this specific
orientation. If the
bar of light is in a
similar
orientation, but
not the exact
preferred
orientation, less
action potentials
will fire.
‘Orientation
selectivity
experiment’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
This orientation selectivity within the visual cortex is the same for all neurons located within a single
cortical column. This means that the cells in each cortical column will respond optimally to a line at
a preferred orientation. So, if a recording electrode is inserted vertically through the cortex to record
from cells within a single cortical column, all cells will respond optimally to the same sensory stimulus.
If the recording electrode is inserted horizontally, such that it records across multiple cortical columns,
each cortical column will respond optimally to a different sensory stimulus.
Figure 30.16.
Orientation
selectivity in
cortical columns.
Here a cross
section of the
cortex is show
with layers I-VI
labeled. In the
left image, a
recording
electrode (black
triangle) is
inserted
vertically into a
single cortical
column (columns
divided by blue
lines). All the cells
in this cortical
column respond
to a bar of light
in the horizontal
orientation.
Adjacent cortical
columns respond
to bars of light at
different
orientations. If a
recording
electrode is
inserted
horizontally, such
that it records
from multiple
different cortical
columns, each
cortical column
will respond to a
bar of light at a
specific
orientation.
‘Orientation
selectivity in
cortical columns’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Ocular Dominance Columns

Another experiment by Hubel and Wiesel determined the existence of ocular dominance columns,
or columns of neurons within the visual cortex that respond preferentially to either the left or right eye.
For this experiment, Hubel and Wiesel injected a radioactive amino acid into one of the eyes of a
monkey. This amino acid then travels by anterograde transport through the retinal ganglion cells of
the eye through the first synapse at the lateral geniculate nucleus of the thalamus, and then through
the next synapse with the striate cortex. Radioactivity within the striate cortex can then be visualized
through the process of autoradiography.
In an autoradiograph image of the striate cortex, there are alternating areas that appear white in color
with areas that appear black in color (similar to the stripes on a zebra). The areas of the cortex that
appear white are the areas that took up the radioactive amino acid and are processing information from
the injected eye. The areas of the cortex that appear black are areas that do not have the radioactive
amino acid and are processing information from the eye that was not injected. Recall, that although
information from the left visual hemifield is processed on the right side of the brain, the left visual
hemifield information is collected from both eyes via the left nasal retina and right temporal retina.
Therefore, both eyes are processed across both the left and right visual cortex.
Figure 30.17.
Illustration of an
autoradiograph
showing ocular
dominance
columns within
the primary
visual cortex.
When a
radioactive
amino acid is
injected into one
of the eyes of a
monkey, the
radioactive
amino acid is
taken up by the
retinal ganglion
cell and travels
through
anterograde
transport to the
primary visual
cortex. Areas of
the cortex that
received
synapses from
the injected eye
and have the
radioactive
amino acid will
be visualized as
white stripes
within the
primary visual
cortex. Areas of
the cortex that
receive
connections from
the eye that was
not injected with
the radioactive
amino acid will
appear black in
the
autoradiograph.
‘Ocular
Dominance
Columns’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Interestingly, in newborn cats that had not had any visual experience, they did not observe the ocular
dominance columns. Instead, they saw that the inputs of the left and right eye overlapped substantially
within the visual cortex. Hubel and Wiesel were interested in understanding how these ocular
dominance columns develop between infancy and adulthood and whether their development was
dependent on visual experience.
For their experiment, they took a kitten immediately after birth, and sewed the right eye of the
kitten closed, so that the eye could no longer have any visual experience, a condition called monocular
deprivation (‘mono’ meaning ‘one’ and ‘ocular’ meaning ‘eye’). After this six-week period of
monocular deprivation, the eye of the cat was reopened so that it could take in the visual environment.
A control group of cats had normal visual experience for this same six-week period.
In control cats that did not experience monocular deprivation, Hubel and Wiesel found that they
developed normal ocular dominance columns at six weeks post birth. The cats that experienced
monocular deprivation for the first six weeks of life, however, showed abnormal development of
the ocular dominance columns. The columns that normally served the right eye (that was sewn
shut) shrank in size, and the neighboring columns that served the left eye grew into the unoccupied
cortical space. Hubel and Wiesel were able to conclude that visual experience was necessary for the
ocular dominance columns to develop. In fact, they found that there was a critical period in cat
visual development in the first six weeks of life where visual experience is required for normal visual
development.
Importantly, these structural changes also translated into functional changes for the vision of the
cat. Cats that experienced monocular deprivation had decreased visual function in the eye that was
sewn shut for the rest of the cat’s life. Even though the sewn shut eye was reopened after the critical
period, the development of the ocular dominance columns in the brain was already complete and thus
the brain could never process visual information from the sewn shut eye.
Figure 30.18.
Development of
ocular dominance
columns. At birth,
ocular dominance
columns are not
present in kittens
(represented by
gray bar). Both
eyes (R and L)
provide equal
visual experience
to the neurons of
the primary
visual cortex
(represented by
the arrows). The
neurons that
serve the cortex
have overlapping
inputs from the
left and right
eyes (shown by
blue and red
neurons). After
six weeks of
normal visual
experience,
ocular dominance
columns are
observed in cats-
that is, there are
areas of the
cortex that
process
information from
either the left or
right eye (shown
by black and
white bars), and
their neuronal
inputs do not
overlap (distinct
areas of red and
blue neurons).
When the right

eye is sewn shut
immediately
following birth
for six weeks,
there is
decreased input
from the sewn
shut eye (skinny
black arrow) and
thus increased
input from the
open left eye
(large white
arrows). This
causes the ocular
dominance
columns to not
develop normally.
The ocular
dominance
columns that
served the sewn
shut eye shrink
in response to
the decrease in
visual experience.
The adjacent
ocular dominance
columns that
serve the left
eye, that
remained
opened, expand
to fill in the
cortical space
that normally
would have been
take up by the
right eye. As a
result, the eye
that was sewn
shut has
decreased brain
area that
processes visual
information from
the eye and
decreased visual
function for the
remainder of the
cat’s life. ‘Ocular
Dominance
Column
development’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Higher-Level Processing of Sensory Information

Sensory system processing of input does not end upon reaching the primary sensory cortex in any
sensory system. Information typically gets sent from the primary sensory cortex to other sensory
association regions throughout the brain. The characteristics of sensory information becomes more
complex as this higher-level processing occurs.
Post-Striatal Processing
In the visual system, there are two broad streams of information that leave the striate cortex. Visual
information passes through two streams of communication: the dorsal stream and the ventral
stream. In the ventral stream, information travels from the primary visual cortex down through
the inferior temporal lobe is responsible for determining object recognition, or what an object is.
Differentiating between an apple and a person occurs in this stream. In the dorsal stream, information
travels from the striate cortex up through the parietal lobe and is responsible for motion or spatial
components of vision.
Figure 30.19. Information continues to be processed after reaching the primary visual cortex. The
dorsal stream travels to the parietal cortex and is important for spatial components of vision. The
ventral stream travels to the temporal lobe and is important for object recognition. ‘Visual Streams’
Dorsal Stream
The dorsal stream is described as the “where” pathway because these structures help us identify where
objects are located in the space around us. One of the most important regions in the dorsal pathway is
region MT, also called V5, which contributes to perception of motion. In this region, neurons are
preferentially activated by a specific direction of movement by an object – for example, left to right or
up to down. As an example, remember the receptive fields in the primary visual cortex were activated
by lines at a specific orientation. Like that, in V5, the neurons would be activated by lines moving in a
specific direction.
As information continues to be processed through the dorsal stream, the neurons become selective
for more complex motions. The dorsal stream is also important for processing our actions in response
to visual stimulation, for example, reaching for an object in the visual field or navigating around objects
while walking.
These structures also guide us when we move through our environments, contributing to our sense
of spatial awareness. For example, a task such as reaching out to grab an object in front of you uses a
combination of these features, so this task is guided largely by dorsal stream structures.
Figure 30.20. Area MT, also called V5, is an early processing region of the dorsal stream through the
parietal lobe. Neurons in the region are activated by direction of an object in a specific direction. ‘Area
MT’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial Share-
Ventral Stream
The ventral stream is the “what” pathway, and helps in the identification of objects that we see. Object
identification is a key function of our visual system. The ventral visual stream is responsible for this
process. Further, ventral stream structures are important for visual memory. Like the more complex
activation characteristics of region MT in the dorsal stream, neurons in Area V4 in the ventral stream
show more complex receptive fields and show sensitivity to shape and color identification. In fact,
there is a rare clinical condition in humans called achromatopsia, in which individuals have partial or
complete loss of color vision. These individuals still have normal functioning cones within the retina,
and normal LGN and primary visual cortex function. Instead, they typically have damage to occipital
and temporal lobes, where the ventral stream is located.
A major output of area V4 is area IT, located within the inferior temporal lobe. As information
travels to area IT it continues to be processed and differentiation of objects occurs. Neurons located in
area IT are important in learning and memory in visual perception (have I seen that before?), and have
been found to respond to colors and shapes.
Another region within the ventral stream called the fusiform face area, located in the fusiform
gyrus, which lies on the ventral aspect of the temporal lobe, contains neurons that are activated by faces
and can be specialized to one specific face. When the visual system senses these complex stimuli, those
signals get processed through these ventral stream pathways. These incoming stimuli are compared
with the memories stored in the ventral stream, and this comparison contributes to our capacity for
perception and identification.
The two streams are not independent of each other. Rather, successful organisms require the
melding of both components of visual perception. For example, imagine you are a prehistoric organism
living in a food-scarce environment. Approaching a small berry tree, you would use ventral stream
structures to correlate the berries with memories: Did these berries taste good and give me the calories
I need to survive? Or did these berries make me violently ill, and are therefore probably poisonous? If
they are the delicious berries that I want, I will use the dorsal stream structures to take note of their
precise location so I can reach out for them and pick the berries . In this example, proper interaction of
the dual streams contributes to goal-driven actions.
Figure 30.21. The ventral stream is first processed by area V4, which recognizes shapes and color.
Information the continues through the inferior temporal lobe and sends information to regions like
the fusiform gyrus, which is an area responsible for the recognition of faces. ‘Ventral Stream’ by
The inferior temporal lobe also makes reciprocal connections with the structures in the limbic system.
The limbic system plays an important role in processing emotions and memory, both of which are
significant components to visual perception. The amygdala ties visual stimuli with emotions and
provides value to objects. A family member will have emotional ties that a stranger will not. The
hippocampus is responsible for learning and memory and helps establish memories of visual stimuli.
Figure 30.22. The limbic system structures, the amygdala and the hippocampus, also play important
roles in visual processing. Both regions are located deep in the temporal lobe and have reciprocal
connections with the ventral stream as is it moves through the temporal lobe. ‘Deep Temporal Lobe’
View the amygdala using the BrainFacts.org 3D Brain

View the hippocampus using the BrainFacts.org 3D Brain
Key Takeaways
• The nasal and temporal retinal regions are responsible for viewing specific regions of
the visual field
• Some retinal projections cross the midline at the optic chiasm, causing the left side of
the brain to process the right visual hemifield and vice versa
• The retinal axons synapse in the lateral geniculate nucleus of the thalamus.
Information then travels to the primary visual cortex
• Receptive fields and the preferred visual stimuli for neuron activation become more
complex as information moves through the visual pathway
◦ Primary visual cortex neurons have linear receptive fields are are activated by a
line in a specific orientation
◦ Area MT / V5 is activated by motion in a specific direction
◦ Area V4 is activated by specific shapes and colors
◦ The fusiform gyrus is activated by faces
• The retina also projects to midbrain regions

• Ocular dominance columns that process information from either the left or right eye
develop during a critical period of development
Test Yourself!
Attributions
Media Attributions
• neural pathway from the retina © Mads00 is licensed under a CC BY-NC-SA (Attribution
• Lateral Geniculate Nucleus © Valerie Hedge is licensed under a CC BY-NC-SA (Attribution
• optic radiation © Mads00 adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Visual pathway © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Human Primary Visual Cortex © Geyer S, Weiss M, Reimann K, Lohmann G and Turner R is
licensed under a CC BY (Attribution) license
• Laminar organization of the cortex © Korbinian Brodmann adapted by Valerie Hedges is
• Cajal Cortex Drawings © Santiago Ramon y Cajal adapted by Valerie Hedges is licensed under a
Public Domain license
• Orientation selectivity © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Orientation selectivity in cortical columns © Valerie Hedges is licensed under a CC BY-NC-SA
• Ocular dominance columns © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Development of ocular dominance columns © Valerie Hedges is licensed under a CC BY-NC-
SA (Attribution NonCommercial ShareAlike) license
396 | AUDITORY SYSTEM: THE EAR
31.
AUDITORY SYSTEM: THE EAR
Our nervous system is equipped with a variety of

specialized biological “tools” that can detect much more
than just photons of light. We can detect the shape of air
Resources waves, and interpreting those signals give us sound
information and the perception of music. In this chapter
we will trace how sounds travel through the structures of
the ear, ultimately causing the auditory receptors to alter
• Glossary Terms their activity and send their signals to the brain.
• Key Takeaways
• Test Yourself
Properties of Sound
Unlike photons of light, sound waves are compressions
and rarefactions of a medium. For us land animals, that medium is usually air, but sound waves can
propagate very well in water or through solids. Before we get to the anatomical structures involved in
sound perception, it is important to first understand the physical nature of sound waves. All sounds,
from the clattering of a dropped metal pan to the melodies of a Mozart violin concerto, are contained
in their corresponding sound waves. Two components of sound waves are frequency and amplitude.
1. Frequency, or “How often do the sound waves compress?” The greater the frequency, the
higher the pitch. The highest notes humans are able to hear is around 20,000 Hz, a painfully-
shrill sound for those who can hear it. People often tend to lose their high frequency hearing as
they age. On the opposite end of the spectrum, low frequency sounds are the deep rumbles of
bass, and the human ear can hear sounds down in the 20 Hz range.
2. Amplitude, or “How much do the waves displace the medium from baseline?” The larger the
amplitude of the wave, or the greater distance between the peak and the trough of the signal, the
AUDITORY SYSTEM: THE EAR | 397
louder the sound is. Loudness is measured in decibels (dB). To give you an idea of approximate
sound intensities, the background noise of a quiet library is about 40 dB, and a typical
conversation is close to 60 dB. A rock concert or lawnmower is between 100 and 110 dB, which
is right around the pain threshold. Prolonged exposure to these high amplitude sound waves can
lead to permanent damage to the auditory system resulting in hearing loss or tinnitus (a ringing
in the ear, even in the absence of a sound stimulus).
Figure 31.1. Sound

Waves. The
frequency of
sound waves is
measured as the
number of peaks
that occur over
time and
corresponds to
the pitch of
sound. Higher
frequency sound
waves have a
higher pitch
whereas lower
frequency
sounds waves
have a lower
pitch. The
amplitude of
sound waves is
measure from
the peak of the
wave to the
trough and
corresponds to
how loud a
sound is. Larger
amplitude waves
are louder,
whereas smaller
amplitude waves
are quieter.
Physical Structures of the Auditory System:

Outer Ear
Our auditory system is a series of physical structures and nervous system components that are
responsible for conveying sound waves into meaning and context.
The external component of the auditory system begins with the pinna. Its shape functions as a
funnel, capturing and channeling sound waves into the auditory canal. The pinna and the auditory
canal are parts of the outer ear. Also, because the pinna is asymmetrical, its shape helps us determine
where a sound is coming from. In some nonhumans, the pinna serves these functions and more. For
instance, some animals are able to disperse excess heat through their ears (elephants), and some even
use them to display emotion (dogs, horses).
At the end of the auditory canal is the tympanic membrane, or ear drum. This membrane is a
very delicate piece of tissue at only 0.1 mm thin and is subject to damage by physical injury such as
head trauma, nearby explosions, or even changes in air pressure during scuba diving. When incoming
sound waves reach the tympanic membrane, it vibrates at a matching frequency, and amplitude. The
tympanic membrane also represents the boundary between the outer ear and the middle ear.
Figure 31.2.
Human outer ear
anatomy. The
outer ear
(highlighted in
brown) consists
of the pinna,
which acts as a
funnel to direct
sound waves
into the auditory
canal. The
tympanic
membrane
separates the
auditory canal
from the middle
ear.
Physical Structures of the Auditory System:

Middle Ear
The middle ear is an air-filled chamber. Physically attached to the tympanic membrane are the ossicles,
a series of three bones that convey that vibrational sound information. These bones in order, called
the malleus, incus, and stapes, conduct vibrations of the tympanic membrane through the air-filled
middle ear. The stapes has a footplate that attaches to a structure called the oval window, which serves
as the junction between the middle ear and the inner ear. The middle ear serves important functions in
both sound amplification and sound attenuation.
Figure 31.3.
Human middle
ear anatomy. The
tympanic
membrane
separates the
outer ear from
the middle ear
(highlighted red
in this image).
The middle ear is
an air filled
chamber that
contains three
small
interconnected
ossicles (bones):
the malleus,
incus, and stapes.
The malleus is
physically
attached to the
tympanic
membrane. The
malleus then
connects to the
incus, and the
incus connects to
the stapes. The
stapes is shaped
like a stirrup and
is connected to
the oval window
that separates
the middle ear
from the inner
ear.
Sound Amplification
The tympanic membrane and the ossicles function to amplify incoming sounds, generally by a tenfold
difference. This amplification is accomplished through 2 mechanisms:
1. Due to the ossicles being connected, the ossicles act in a lever-like fashion to amplify the
movements of the tympanic membrane to the oval window
2. The stapes has a smaller area on the oval window than the tympanic membrane, thus
movements of the larger tympanic membrane must be transformed into smaller and stronger
vibrations at the oval window.
This amplification is important because the inner ear is filled with liquid rather than air, and sound
waves do not travel very well when moving from air into a denser medium – think about how muffled
sounds are when you submerge your head underwater.
Sound Attenuation
The movement of the ossicles are partially regulated by two different muscles, the tensor tympani
muscle which connects with the malleus, and the stapedius muscle which connects to the stapes.
When these muscles contract, it causes the ossicles to be more rigid and for the ossicles to move less,
which decreases the intensity of loud sounds. This response, called the acoustic reflex, dampens
incoming sound by about 15 dB. (This is why we talk much louder than normal when we first leave a
concert: we have lessened auditory feedback from our ears, so we tend to talk louder to compensate.)
The muscles contract at the onset of loud noises with a slight delay of 50-100ms.
Physical Structures of the Auditory System: Inner

Ear
The inner ear is a fluid-filled structure made up of two structures: the cochlea that functions in
hearing, and the semicircular canals that function in balance.
The auditory part of this structure is a small spiral-shaped structure about the size of a pea, called the
cochlea (cochlea is named for the Ancient Greek word “snail shell”.) The cochlea has two small holes
at its base: the oval window and the round window.
Figure 31.4.
Human inner ear
anatomy. The
inner ear has two
structures: the
semicircular
canals and the
spiral-shaped
cochlea. The
structures of the
inner ear are
filled with fluid.
They are
separated from
the air-filled
middle ear by
two membranes
called the oval
window and the
round window.
Cross Section of the Cochlea

When we examine a cross-section of the cochlea, we can see that there are 3 distinct fluid-filled
chambers called the scala vestibuli, the scala media, and the scala tympani. These chambers are
separated from each other by membranes.
The fluid found within the scala vestibuli and scala tympani is called perilymph and it has a low
concentration of potassium and a high concentration of sodium. The scala media is filled with a fluid
called endolymph that has a high concentration of potassium and a low concentration of sodium.
Reissner’s membrane separates the scala vestibuli from the scala media. The basilar membrane
separates the scala tympani from the scala media.
Figure 31.5.
Cochlea cross
section. In cross
section, the three
chambers of the
cochlea can be
observed.
Reissner’s
membrane
separates the
scala vestibuli
from the scala
media. The
basilar
membrane
separates the
scala media from
the scala
tympani. The
Organ of Corti is
embedded within
the basilar
membrane and
extends into the
scala media.
‘Cochlea Cross
Section’ by
Valerie Hedges is
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Basilar Membrane
Think of the cochlea as a rolled-up cone. If this cone was theoretically unrolled, the widest diameter
portion, called the base, would be closest to the oval window, while the narrowest portion, called the
apex, would be at the center of the spiral.
The basilar membrane runs down the middle of the cochlea. The width of the basilar membrane
changes as it runs down the length of the cochlea from the base to the apex. This change in shape from
the base to the apex is important: objects with different stiffness vibrate at different frequencies. The
base of the cochlea is stiff and rigid and will vibrate at high frequencies. Whereas the apex is wider and
less stiff, so it vibrates at lower frequencies.
Because different frequencies of sound affect different areas of the basilar membrane, the basilar
membrane is what is referred to as tonotopically organized. In fact, you can think about the way that
frequencies are mapped to the basilar membrane similar to a backwards piano.
Figure 31.6.
Basilar
membrane.
When the
cochlea is
uncoiled, it is
easier to see the
different
structures. The
basilar
membrane runs
down the middle
of the cochlea.
The oval window
and round
window are
located at the
base of the
cochlea and the
stapes foot plate
connects to the
oval window.
The helicotrema
is a hole in the
basilar
membrane at the
apex of the
cochlea, allowing
the perilymph of
the scala
vestibuli to
connect to the
perilymph of the
scala tympani. by
Valerie Hedges is
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Let’s consider how sounds will affect the cochlea. For simplicity’s sake, the figure here shows only the
scala vestibuli and the scala tympani (not the scala media) with the basilar membrane running down
the middle. Keep in mind that due to the flexibility of Reissner’s membrane that separates the scala
vestibuli from the scala media, we can assume that pressure changes in the scala vestibuli are transferred
through the scala media, ultimately affecting the basilar membrane.
1. When sounds move through the outer and middle ear, it causes vibration of the stapes footplate
at the oval window. The movement of the stapes at the oval window is similar to a piston
pushing at the oval window at the same frequency and amplitude as the incoming sound.
2. When the stapes pushes into the oval window, it causes movement of the perilymph within the
scala vestibuli.
3. This causes the sounds to move through the cochlea as a wave from the base to the apex,
displacing the flexible basilar membrane at different locations dependent on the frequency of
sound.
4. At the apex of the basilar membrane is a hole called the helicotrema that connects the perilymph
of the scala vestibuli to the perilymph of the scala tympani and allows for the pressure to be
transferred from the scala vestibuli to the scala tympani.
5. The pressure then moves through the scala tympani back towards the base of the cochlea until it
pushes out at the round window.
Figure 31.7.
Basilar
membrane
displacement.
The basilar
membrane runs
down the middle
of the cochlea.
The stapes
pushes in at the
oval window to
displace the
perilymph of the
scala vestibuli.
The movement
of the fluid
causes the
flexible basilar
membrane to be
displaced like a
wave. The
pressure moves
through the fluid
of the scala
vestibuli and
then around the
helicotrema and
back through the
fluid of the scala
tympani,
displacing the
round window.
‘Basilar
Membrane
Displacement’ by
Valerie Hedges is
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The Organ of Corti

Embedded within the basilar membrane is a structure called the Organ of Corti. The Organ of Corti
is the first nervous system structure that is responsible for processing physical vibrations and converting
them into signals that the nervous system can interpret. The Organ of Corti contains the components
necessary for converting sound waves into action potentials. Recall that the scala media that surrounds
the Organ of Corti contains endolymph, a fluid that has a high concentration of potassium and a low
concentration of sodium.
A separate membrane hangs over the Organ of Corti called the tectorial membrane. Recall that
“tectum” means roof. The tectorial membrane acts as a roof over the Organ of Corti.
Embedded along the interior surface of the Organ of Corti are the somata of hair cells, the primary
sensory neurons that interpret physical movement. They are named “hair cells” because of their cellular
structure; each hair cell has somewhere between 30 and a few hundred hair-shaped stereocilia that
protrude away from the Organ of Corti, reaching into the endolymph.
Importantly, hair cells are not neurons. They do not produce action potentials and do not have
axons. In fact, hair cells are a specialized type of epithelial cell. We have two different populations of
hair cells, the inner hair cells and outer hair cells.
• The outer hair cells are arranged in three rows. Their stereocilia extend into the endolymph and
are physically embedded within the tectorial membrane.
• The inner hair cells are arranged in a single row. Their stereocilia extend into the endolymph but
are not embedded within the tectorial membrane. Instead, the stereocilia of the inner hair cells
freely float within the endolymph.
Although the hair cells themselves are not neurons, the mechanical bending of the hair cell stereocilia
will be converted into a neural signal. Hair cells synapse on spiral ganglion cells (neurons). The axons
of the spiral ganglion cells make up the auditory nerve, which will project into the cochlear nuclei of
the medulla.
Figure 31.8.
Organ of Corti.
The Organ of
Corti is
embedded in the
basilar
membrane. There
are three rows of
outer hair cells
and one row of
inner hair cells.
The tectorial
membrane
extends over the
hair cells. The
hair-like
projections of the
outer hair cells
are embedded
within the
tectorial
membrane,
whereas the
hair-like
projections of the
inner hair cells
are not
embedded within
the tectorial
membrane.
‘Organ of Corti’
by Valerie
Hedges is
licensed under a
Creative
Commons
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Neural Components of the Auditory System

As we have learned, when a vibration reaches the oval window, this causes the basilar membrane to
move in response to the change in pressure. The basilar membrane is located at the base of the hair cells,
and as the basilar membrane moves up and down, it pushes the hair cell stereocilia into the tectorial
membrane above, causing the stereocilia to bend.
Figure 31.9.
Basilar
membrane
movement
causes hair cell
stereocilia to
bend. The
movement of the
basilar
membrane in
response to
sound causes the
hair cell
stereocilia to be
pushed into the
stationary
tectorial
membrane,
causing them to
bend. ‘Basilar
movement bends
stereocilia’ by
Valerie Hedges is
licensed under a
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Hair Cells
Let’s take a closer look at the structure of the hair cell.

The stereocilia of the hair cell have different lengths and are arranged from shortest to tallest. At
the tip of each stereocilium are mechanically-gated ion channels. These are mechanically-gated because
they open and close in response to physical movement. The stereocilia are linked together with spring-
like proteins called linker proteins. These linker proteins are specifically attached to small covers that
block movement through mechanically-gated ion channels when closed.
The stereocilia can bend in two different directions, toward the shortest stereocilium or towards the
tallest stereocilium. At rest (no sound), the stereocilia are not bent. Some of the mechanically-gated ion
channels are open, and some are closed.
Figure 31.10. Hair

cell at rest. When
the stereocilia are
not being bent
some of the
mechanically-gat
ed ion channels
at the tips of the
stereocilia are
open and others
are closed, and
the cell is neither
depolarized or
hyperpolarized.
When the hair
cell is at rest,
synaptic vesicles
do not release
neurotransmitter
s at the spiral
ganglion cell
synapse. ‘Hair
cell at rest’ by
Valerie Hedges is
licensed under a
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When the stereocilia bend towards the tallest stereocilium, the distance between the stereocilia
increases and the spring-like linker proteins are stretched, causing the opening of the covers on the
mechanically-gated ion channels.
Recall that the stereocilia are surrounded by endolymph, which is high in potassium and low in
sodium. When the mechanically-gated ion channels are opened, potassium ions will flow into the hair
cells, moving down its concentration gradient. As the positively charged potassium ions move into the
hair cell, it causes the hair cell to depolarize.
If the stereocilia instead bend towards the shortest stereocilium, the distance between the stereocilia
gets smaller. The mechanically-gated ion channels cannot open and the channels that were previously
opened when the cell was at rest, will now be shut. As a result, there is less potassium influx into
the hair cell and thus less positive charge in the hair cell than there was at rest, leading to hair cell
hyperpolarization.
Figure 31.11 Hair

cell at rest, in a
depolarized state,
and a
hyperpolarized
state. When the
hair cell is at rest,
some of the
mechanically-gat
ed ion channels
at the tips of the
stereocilia are
open and some
are closed (left).
When the
stereocilia are
bent in the
direction of the
tallest
stereocilium the
linker proteins
open the
mechanically-gat
ed ion channels.
When
mechanically-gat
ed ion channels
are open,
potassium flows
into the cell
down its
concentration
gradient,
allowing for a
large influx of
potassium ions
leading to
depolarization of
the hair cell
(middle). When
the stereocilia are
bent in the
direction of
shortest
stereocilium the
linker proteins
close the
mechanically-gat
ed ion channels,
no longer
allowing for
potassium influx.
This leads to the
hair cell being
hyperpolarized
(right). ‘Hair cell
at rest, in a
depolarized state,
and a
hyperpolarized
state’ by Valerie
Hedges is
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Hair Cell Signaling
Located within the hair cell are vesicles that are filled with glutamate (an excitatory neurotransmitter).
When hair cells are depolarized, this causes the opening of voltage-gated calcium channels in the hair
cell membrane. The influx of calcium will ultimately cause the vesicles full of glutamate to fuse with
the hair cell membrane and release the glutamate into the synapse with the spiral ganglion cell.
Figure 31.12.
Depolarized hair
cell. When the
stereocilia bend
in the direction
toward the
longest
stereocilium, this
stretches the
linker proteins
and opens the
mechanically-gat
ed ion channels
at the tips of the
stereocilia.
Potassium flows
into the cell,
down its
concentration
gradient,
depolarizing the
hair cell. This
change in
membrane
potential opens
voltage-gated
calcium channels.
The influx of
calcium into the
hair cell causes
synaptic vesicles
to fuse with the
membrane and
release
glutamate (red
circles) into the
synapse with the
spiral ganglion
cell. ‘Depolarized
hair cell’ by
Valerie Hedges is
licensed under a
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Spiral Ganglion Cell Innervation

Most of the spiral ganglion cells (>95%) collect information from the inner hair cells. In fact, one
inner hair cell will synapse onto many different spiral ganglion cells. Whereas a small number of
spiral ganglion cell (<5%) collect information from the outer hair cells. Thus, the inner hair cells are
responsible for sending the majority of auditory signals from the cochlea into the brain for processing.
Figure 31.13. Hair

cell innervation.
Many spiral
ganglion cells
collect
information from
the inner hair
cells, whereas
only a small
number of spiral
ganglion cells
collect
information from
the more
numerous outer
hair cells. The
axons of spiral
ganglion cell
make up the
Auditory nerve.
‘Hair cell
innervation’ by
Valerie Hedges is
licensed under a
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Cochlear Amplifier
Although the outer hair cells are not responsible for the majority of the signal to the spiral ganglion
cells, they serve another function. The outer hair cells function as the cochlear amplifier to increase
the intensity of vibrations within the cochlea. It is estimated that the outer hair cells increase sound by
anywhere between 20 and 80 dB.
There is a motor protein called prestin within the outer hair cell membrane. As a motor protein,
prestin can mechanically contract and elongate within the outer hair cells, changing the length of the
outer hair cell. The membrane of the outer hair cell shortens and lengthens with the movement of the
basilar membrane. When the basilar membrane is displaced upwards, this causes the prestin protein to
contract, further shortening the length of the outer hair cell. When the basilar membrane is displaced
downward, the prestin protein elongates the length of the outer hair cell. Essentially, the prestin within
the outer hair cells amplifies the movement of the basilar membrane in both directions.
When the cochlear amplifier is functional, it doesn’t only change the displacement of the basilar
membrane, but it also has effects on the inner hair cells. The cochlear amplifier will cause the inner
hair cells to bend more than they would without the amplifier present. In this way, although the outer
hair cells have fewer direct signals to the spiral ganglion cells, they still contribute to the firing of the
auditory nerve through their influence on the inner hair cells.
Hearing Loss
Many people experience permanent hearing loss, a decrease in volume by 25 dB or more.

Hearing loss is divided into two categories.
Conductive hearing loss is a result of changes to the auditory system up to the oval
window, such as a tumor in the ear canal, a perforation of the tympanic membrane, or
changes in middle ear pressure (such as how everything sounds muffled while changing
altitudes when an airplane takes off, for example).
Sensorineural hearing loss results from changes at the level of the inner ear or further up
in the neural pathway, such as hair cell damage, a brain tumor, bacterial or viral infections,
or exposure to various toxins or drugs.
The most common cause of hearing loss is excessive noise exposure. Although the acoustic
reflex is capable of dampening the intensity of the incoming vibrations, prolonged exposure
to high amplitude sound waves can still cause damage. Motorcycles, the maximum volume
on headphones, or loud venues like concerts and clubs can produce sounds in the 95-110 dB
range, which can cause some permanent hearing loss. Additionally, the acoustic reflex is not
fast enough to minimize damage from sudden, loud sounds in excess of 120 dB, such as a
gunshot. All of these sources of acoustic trauma are preventable by wearing appropriate
hearing protection, which can decrease the intensity of sounds by up to 30 dB. Old age is
another common cause of hearing loss, likely because older people have had more
accumulated exposure to noise.
An estimated 1 in 3 people older than 65 have hearing deficits. We are born with about
15,000 hair cells, but throughout the course of our life, many get damaged irreparably. Hair
cells at the base of the cochlear are more sensitive to injury, so it is common for people to
lose sensitivity to high-frequency sounds. The loss of these hair cells can begin as early as a
person’s 20s. Partial hearing loss can be reversed with the help of medical devices. A
hearing aid is a processor that helps to filter out background noise, decrease pitch, and
amplify incoming sounds.
A cochlear implant is a surgically-implanted device that receives incoming sound

information and directly stimulates the auditory nerve via electrodes, bypassing the
external components of the auditory system.
Key Takeaways
• The ear anatomy can be divided into the air-filled outer and middle ear, and the fluid-
filled inner ear
• The cochlea within the inner ear is a chambered structure that contains the
tonotopically-arranged basilar membrane and the Organ of Corti.
• Sounds displace the basilar membrane and bend hair cells in different directions,
causing either depolarization or hyperpolarization depending on the direction of
movement
• Outer hair cells and inner hair cells are differentially innervated, with many more
spiral ganglion cells collecting information from the inner hair cells than the outer hair
cells
• The outer hair cells are called the ‘cochlear amplifier’ due to their ability to contract
and ‘amplify’ the movement of the basilar membrane, thus allowing more signals to
the inner hair cells
Test Yourself!
Attributions
Media Attributions
• Sound Wave © Kulayada adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Outer Ear © Chittka L Brockmann adapted by Valerie Hedges is licensed under a CC BY

• Middle Ear © Chittka L Brockmann adapted by Valerie Hedges is licensed under a CC BY
• Inner Ear © Chittka L Brockmann adapted by Valerie Hedges is licensed under a CC BY
• Cross section of cochlea © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Basilar membrane © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Basliar membrane displacement © Valerie Hedges is licensed under a CC BY-NC-SA
• Organ of Corti labeled © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Movement of basilar membrane bends hair cells © Valerie Hedges is licensed under a CC BY-
• Hair cell at rest © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Hair cell depolarization and hyperpolarization © Valerie Hedges is licensed under a CC BY-NC-
• Depolarized hair cell © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Hair cell innervation © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
AUDITORY SYSTEM: CENTRAL PROCESSING | 423
32.
AUDITORY SYSTEM: CENTRAL

PROCESSING
All sensory systems follow the same general path of

communication into our nervous systems and awareness.
First, the incoming signal must reach a that can change its
electrical properties in response to the stimulus. Then, Resources
that information initiates a series of signals into the CNS,
reaching structures such as the thalamus, primary sensory
cortical areas, and finally, higher order perception.
Although there are several sensory components • Glossary Terms
throughout our body that detect these signals, there are • Key Takeaways
no sensory receptors in our central nervous system. We • Test Yourself
previously saw this pattern for the visual system, and now
we’ll see it mirrored in many ways by the auditory system.
For the auditory system, following the activation of hair
cells within the cochlea, sound information is transmitted to brain structures including the thalamus
and cortex for processing to be perceived as sounds.
Central Auditory Pathway

Hair cells within the cochlea form glutamatergic synapses onto spiral ganglion cells. The axons of these
neurons make up the vestibulocochlear nerve (Cranial nerve VIII). Neurons project to and synapse
on neurons within the ipsilateral cochlear nuclei of the medulla. Because the medulla neurons only
process sound from one ear, these cells are referred to as monaural. (one ear).
The cochlear nuclei neurons within the medulla have axons that split and synapse on both the
ipsilateral and contralateral superior olive within the pons. The superior olive is the first structure
424 | AUDITORY SYSTEM: CENTRAL PROCESSING
that processes sound information from both ears, so it is referred to as binaural. All remaining
structures in the central auditory pathway process sounds from both ears, and thus are also binaural.
From the pons, neurons travel via the lateral lemniscus (a lemniscus is a collection of axons)
and then synapse on the inferior colliculus of the midbrain tectum. Signaling within the inferior
colliculus is important for interactions between multiple sensory inputs and a motor response. These
inferior colliculus neurons are particularly responsive to biologically-relevant sounds, such as
unexpected noises, which may signal an approaching predator. Processing in the inferior colliculus
helps the animal focus their attention on these stimuli.
The inferior colliculus then conveys that auditory information into the medial geniculate nucleus,
one of the nuclei of the thalamus. These thalamic neurons then send projections into the primary
auditory cortex located dorsally in the temporal lobe.
Figure 32.1.
Central auditory
pathway. Cranial
nerve VIII
carrying auditory
information
synapses onto
cells of the
ipsilateral
cochlear nuclei of
the medulla. The
cochlear nuclei
neurons within
the medulla have
axons that split
(shown in light
blue) and
synapse on both
the ipsilateral
and contralateral
superior olivary
complex within
the pons.
Neurons then
travel via the
lateral lemniscus
to the inferior
colliculus. The
inferior colliculus
then conveys
auditory
information into
the medial
geniculate
nucleus of the
thalamus. The
thalamic neurons
then send
projections into
the primary
auditory cortex in
the temporal
lobe.
Tonotopy
The structures responsible for the central processing of sound, including the basilar membrane, the
spiral ganglion, the cochlear nucleus, the inferior colliculus, and the auditory cortex are tonotopically
organized, meaning that adjacent physical areas are responsible for conveying information from
adjacent frequencies. For example, the hair cells that respond most to 440 Hz vibrations are right next
to cells that respond maximally to 441 Hz, but far away from cells that respond most to 14,000 Hz.
Likewise, in the auditory cortex, the cells that best process 440 Hz are adjacent to those that best
process 441 Hz, but far away from those that maximally respond to 14,000 Hz.
Figure 32.2.
Tonotopy within
the auditory
system.
Structures
throughout the
central auditory
pathway are
tonotopically
organized. Hair
cells within the
basilar
membrane
respond to
different
frequencies, with
hair cells located
nearer the base
responding to
high frequency
sounds and hair
cells located
nearer to the
apex responding
to low frequency
sounds. This
organization is
conserved
through the
spiral ganglion
cells and within
the cochlear
nucleus, with
adjacent physical
areas responsible
for conveying
information from
adjacent
frequencies.
‘Tonotopic
Organization’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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Auditory Cortex
The auditory cortex (also called A1) is located within the temporal lobes in both hemispheres of the
brain. Similar to the visual cortex, the auditory cortex is also made up of 6 layers of cells that have
columnar organization. Each cortical column responds to a specific frequency. The auditory cortex is
tonotopically organized like the other structures in the central auditory pathway. It is organized as
isofrequency bands, like strips, that respond to relatively the same frequency.
Figure 32.3.
Primary auditory
cortex. The
primary auditory
cortex is located
within the
temporal lobes of
the brain. It is
tonotopically
organized,
maintaining the
tonotopic
organization of
the cochlea, into
isofrequency
bands of cortex
that each
respond to
relatively the
same frequency
of sound
(frequencies
shown in kHz).
Sound Localization
Sound localization is an important function for animals. We can localize sounds in the horizontal plane
and the vertical plane via different mechanisms.
Localizing Sounds in the Horizontal Plane

When considering how we localize sounds in the horizontal plane, we assume that the sound does
not change in elevation. As humans, our ears are located on the sides of our heads, which means that
sounds will arrive at our ears at different times depending on the origin of the sound. The time between
when sound reaches the first ear until the time it reaches the second ear is referred to as the interaural
time difference (ITD).
If we assume that there are 20 cm between the two ears of a human, then we can determine the ITD
for the left ear. If a sound is coming directly from the right, then there will be a 0.6 msec ITD for the
left ear. If sounds are coming from directly in front of a person, then the time that it takes to reach the
right ear will be exactly the same as the time that it takes to reach the left ear, thus there is no ITD (0
msec). If we consider an intermediary angle of sound origin coming from 45° we can determine that
the ITD will be 0.3 msec, exactly between the values from when sounds originate directly in front and
directly to the right.
In order for us to locate sounds in the horizontal plane, we need a brain structure that can process
information from both ears. Recall from the central auditory pathway that the first structure that
processes information from both ears (binaural) is the superior olive.
Figure 32.4.
Sound
localization in the
horizontal plane.
Sounds in the
horizontal plane
reach the ears at
different times
creating an
interaural time
delay between
the ears. When
sound waves are
coming from the
right, the sound
waves reach the
right ear before
they reach the
left ear. If we
assume that
there is a 20 cm
distance
between the
ears, then the
interaural time
delay for the left
ear when sounds
are coming
directly from the
right, will be 0.6
msec. Sounds
that come from
directly in front
of the head will
reach both ears
simultaneously,
and thus there
will be a 0 msec
interaural time
delay. A sound
originating at an
intermediate
angle, directly
between in front
and to the right,
will have a 0.3

msec interaural
time delay for
reaching the left
ear. ‘Sound
localization in the
horizontal plane’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
One of the primary functions of the superior olive is to help us figure out if a sound originates from
the left or the right side of our head by determining this interaural time difference. Let’s imagine a
sound wave originating from the left. The sound will interact with the left ear before it interacts with
the right ear. When the sound interacts with the left ear, it will activate the hair cells in the left cochlea,
the left spiral ganglion, and then the left cochlear nucleus (all monaural structures). The left cochlear
nucleus cells will generate action potential that will travel along their axons toward the superior olive.
After a short delay, the sound wave will interact with the right ear, thus activating the hair cells of the
right cochlea, the right spiral ganglion, and the right cochlear nucleus. The right cochlear nucleus will
then generate its own action potential that will travel toward the superior olive. While this is occurring,
the action potential generated by the left cochlear nucleus has traveled further along the left cochlear
nucleus cell axon.
The action potentials generated from both the left and right cochlear nucleus will converge within
the superior olive (a binaural structure). The axons of the cochlear nucleus cells have different length
paths before they reach the neurons of the superior olive, allowing them to act as ‘delay lines’. The
neurons of the superior olive act as coincidence detectors. When the action potentials arrive at
one superior olive neuron at the same time, the postsynaptic potentials will summate, leading to
maximal activation of this superior olive neuron and the generation of an action potential. If the
action potentials do not arrive at the same time, then summation will not occur. The specific superior
olive neuron that experiences the summation translates to where in the horizontal plane the sound
originated, allowing us to localize sounds.
Figure 32.5.
Interaural time
differences are
determined by
the superior
olive. When a
sound is coming
from the left, the
sound will
activate the
structures of the
left ear. An action
potential will be
fired by the left
cochlear nucleus
neurons (shown
in blue) that will
be carried down
to the superior
olive (top panel).
The left cochlear
nucleus neuron
axon branches to
synapse on the
cells of the
superior olive,
labeled 1-5 for
this example. The
path that the
action potential
will travel to
reach each of
these cells is a
different length,
thus it will take
different
amounts of time
for the action
potential to
travel to each
superior olive
cell. After a brief
time, the sound
wave will reach
the right ear and
activate the
structures of the
right ear (middle
panel). The
action potential
generated by the
neurons of the
right cochlear
nucleus will
travel down their
axons (shown in
red). While this is
occurring, the
action potential
that is coming
from the left is
still traveling
down the blue
axons. When the
action potentials
arrive at the
same cell
simultaneously
from both the
left and right
cochlear nucleus,
the postsynaptic
potentials will
summate and
maximally excite
cell number 4 in
this example
(bottom panel).
‘Superior Olive’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Humans are better at localizing sounds in the horizontal plane when we can detect the onset of the
sound. However, when there are continuous tones, we need to utilize different ways to localize sounds
in the horizontal plane. We can use interaural intensity (loudness) differences to localize sounds when
they are continuous. Sounds are louder the closer you are to them. A difference in volume between
what one ear and the other ear perceives can also be evaluated by neurons in the superior olive.
Interaural intensity differences are also useful in localizing high frequency sounds in the
horizontal plane. At high frequencies (that have a wavelength shorter than the distance between our
ears), the head creates a ‘sound shadow’ for the ear opposite the sound. When a high frequency sound
is coming directly from the right side, the head creates a sound shadow for the left ear, making the
sound louder for the right ear than it is for the left ear. Again, if a high frequency sound originates from
directly in front of a person, then the sound will reach the ears with the same intensity, as the sound
shadow will be behind the head. When a high frequency sound originates from an intermediate angle,
then there will be intermediate intensity differences between the two ears.
Figure 32.6.
Interaural
intensity
differences. High
frequency
sounds that have
a wavelength
shorter than the
distance
between the ears
will cause the
head to create a
sound shadow. A
sound shadow
will decrease the
intensity
(volume) of the
sound in the ear
opposite to the
sound origin.
When a high
frequency sound
is coming from
the right, the
head will create a
sound shadow
for the left ear,
causing the left
ear to hear a
lower intensity
sound. When a
sound comes
from directly in
front of a person,
the sound
shadow will be
behind the head
and the intensity
of the sound will
be the same
between the left
and right ear.
Sounds that
come from an
intermediate
angle will cause

an intermediate
intensity
difference
between the
ears. ‘Interaural
intensity
differences’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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International
License.
Low frequency sounds do not create a sound shadow like high frequency sounds. This is because
the wavelength of low frequency waves (e.g. 200 Hz) have a wavelength longer than the width of the
distance between the ears, so the intensity will be the same at both ears but the sound will arrive at the
two ears at different times (interaural time difference).
High frequency sounds (e.g. 7000 Hz) have a wavelength that is shorter than the width of the
distance between the ears, which is why the head creates a sound shadow that will alter the intensity of
the sound between the two ears.
Interaural time difference is used for low frequency sounds in the 20-2000 Hz range, whereas
interaural intensity difference is used for high frequency sounds in the 2000-20,000 Hz range.
Together these two processes are called the duplex theory of sound localization.
Figure 32.7. High

frequency versus
low frequency
sound
localization in the
horizontal plane.
High frequency
sounds have a
shorter
wavelength and
cause the head
to create a sound
shadow that will
make the sounds
different
intensities
between the two
ears (left image).
Low frequency
sounds have a
longer
wavelength that
the head does
not interfere
with, thus the
head does not
create a sound
shadow. Instead,
the sound waves
reach the ears at
different times,
creating an
interaural time
difference (right
image). ‘High
frequency versus
low frequency
sound
localization’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Localizing Sound in the Vertical Plane

Because both ears (in humans and most non-humans) are at the same height, sounds in the vertical
plane that change in elevation alone can be difficult to localize. This is because the interaural time
difference and interaural intensity between the two ears remains constant as elevation changes. This is
why sometimes people and animals tilt their heads to try to hear better.
The structure of our pinna is important in vertical localization. Recall that our pinna is the external
portion of our ear that funnels sounds into the auditory canal. For sound localization, the pinna has
many ridges and folds, which cause sound waves to be reflected off these bumps and take different
paths on their way to the auditory canal. The pinna of humans is relatively fixed in location, but other
animals (like dogs) have the ability to move their pinna to better localize sounds.
Interestingly, not all animals have ears that are located at the same height on their head. Some species
of owls have ear openings at different heights on their heads, increasing their ability to localize the
source of a sound on the vertical axis. By having ears at different heights, the owls are able to use
interaural time differences in the vertical plane because the sound will reach their ears at different times.
Figure 32.8.
Vertical plane
sound
localization. The
pinna is critical
for sound
localization in the
vertical plane.
Sounds take
different paths
and are reflected
around the folds
and bumps of
the pinna that
help us localize
sounds in the
vertical plane.
The human pinna
has a fixed
location which
decreases our
ability to locate
sounds in the
vertical plane
compared to
species that have
the ability to
move their pinna.
‘Vertical plan
sound
localization’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Key Takeaways
• Cranial nerve VIII carries auditory information into the cochlear nucleus, superior
olive, inferior colliculus, medial geniculate nucleus of the thalamus, and finally to the
primary auditory cortex.
• Structures of the central auditory pathway are tonotopically organized.
• In the horizontal plane, low frequency sounds are localized through interaural time
differences and high frequency sounds are localized through interaural intensity
differences.
• The superior olive is responsible for calculating interaural time differences.
• Sound localization in the vertical plane is more difficult for humans, but is aided by
the structure of the pinna.
Test Yourself!
Attributions
Media Attributions
• Central Auditory Pathway © Jonathan E. Peelle is licensed under a CC BY (Attribution) license

• Tonotopic organization © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Auditory Cortex © Chittka L, Brockmann adapted by Valerie Hedges is licensed under a CC BY
• Localizing sound © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Superior Olive © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Interaural intensity difference © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• High and Low frequency sounds © Valerie Hedges is licensed under a CC BY-NC-SA
• localizing sound in vertical plane © Valerie Hedges is licensed under a CC BY-NC-SA
VESTIBULAR SYSTEM | 443
33.
VESTIBULAR SYSTEM
When we tilt our head to the side, or look up and down,

that movement information is conveyed to our brain
using the vestibular system. The vestibular system is a sort
of three-dimensional compass that can detect head Resources
movement, and that information helps us figure out how
our head is oriented and how to balance ourselves in
changing conditions. The vestibular system is made up of
two structures that are intimately tied in with the • Glossary Terms
anatomical features of the inner ear. • Key Takeaways
Adjacent to the cochlea within the inner ear is a • Test Yourself
structure called the vestibular labyrinth made up of the
otolith organs (saccule and utricle) and the
semicircular canals.
Otolith Organs
Next to the cochlea and within the vestibular labyrinth are two membranous sacs, the saccule and the
utricle. Collectively, these structures are called the otolith organs and are responsible for determining
gravity through the tilt of the head and linear acceleration. These structures are centrally located
within the vestibular labyrinth.
Due to their differences in structure, the two otolith organs have slightly different functions. The
saccule is more sensitive to vertical movements, like when you are standing in a moving elevator. The
utricle is more responsive to horizontal movements, such as when driving.
444 | VESTIBULAR SYSTEM
Figure 33.1
Structures of the
inner ear. The
semicircular
canals and otolith
organs (utricle
and saccule) are
part of the
vestibular
labyrinth that
functions in
balance. The
cochlea is also
located within
the inner ear and
has auditory
system function.
Similar to the other inner ear structure, the cochlea, the structures of the vestibular labyrinth also
contain hair cells. The hair cells located within the otolith organs are biologically similar to the hair cells
in the cochlea. These hair cells have stereocilia that extend into a gelatinous cap that contains otoconia,
which are small crystals of calcium carbonate. The otolith organs are important in sensing gravity.
Tilting the head causes the otoconia to move (due to gravity), which will push the hair cell stereocilia
and cause them to bend.
Figure 33.2.
Otolith organ
hair cells. Within
the otolith
organs (the
saccule and the
utricle) there are
hair cells that
have stereocilia
that extend into
a gelatinous cap.
Over the
gelatinous cap
are otoconia
(calcium
carbonate
crystals). When
the head is tilted,
the movement of
the otoconia
mechanically
bends to
stereocilia. The
hair cells of the
otolith organs
send their signals
to Scarpa’s
ganglion axons
that transmit
vestibular
information into
the central
nervous system.
‘Otolith organ
hair cells’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
The tallest stereocilium (singular of stereocilia) is called the kinocilium. Similar to the function of
hair cells within the cochlea, movement that bends the stereocilia toward the kinocilium results in
depolarization due to potassium influx through open mechanically-gated ion channels within the
tips of the stereocilia. Movement that bends the stereocilia away from the kinocilium causes the
mechanically-gated ion channels to close, decreasing potassium influx and causing hyperpolarization.
Figure 33.3. Hair

cell at rest, in a
depolarized state,
and a
hyperpolarized
state. When the
hair cell is at rest,
some of the
mechanically-gat
ed ion channels
at the tips of the
stereocilia are
open and some
are closed (left).
When the
stereocilia are
bent in the
direction of the
tallest
stereocilium the
linker proteins
open the
mechanically-gat
ed ion channels.
When
mechanically-gat
ed ion channels
are open,
potassium flows
into the cell
down its
concentration
gradient,
allowing for a
large influx of
potassium ions
leading to
depolarization of
the hair cell
(middle). When
the stereocilia are
bent in the
direction of
shortest
stereocilium the
linker proteins
close the
mechanically-gat
ed ion channels,
no longer
allowing for
potassium influx.
This leads to the
hair cell being
hyperpolarized
(right). ‘Hair cell
at rest, in a
depolarized state,
and a
hyperpolarized
state’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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License.
Semicircular Canals
The other major structure of the vestibular labyrinth are the semicircular canals. The semicircular
canals are the structures that are responsible for detecting head rotation and angular acceleration.
Anatomically, the semicircular canals are a series of three arch-shaped membranous tubes within
the vestibular labyrinth, each one oriented at a right angle to each other. Because of this shape, the
semicircular canals sense and convey information about any direction of head movement.
These semicircular canals are filled with endolymph, the same potassium-rich solution that is in
the cochlea that is important for auditory sensation. At the end of each of the three canals is a small
swelling called the ampulla. Contained in the ampulla is a gelatinous membrane called the cupula.
Here, hair cells extend stereocilia into the cupula. The tallest stereocilium is again referred to as the
kinocilium.
When we rotate our head, the endolymph within the semicircular canals has a delay in movement
due to inertia, and as a result the endolymph will move the cupula in the opposite direction of
head movement. The physical movement of the cupula also pushes the cilia of the hair cells within
the cupula. As previously discussed in the cochlea and the otolith organs, these hair cells also have
mechanically-gated ion channels located in the tips of the stereocilia and function in the same way, with
deflection towards the kinocilium resulting in depolarization and deflection away from the kinocilium
resulting in hyperpolarization.
When we stop rotating, the endolymph within the semicircular canals continues to move for a brief
period of time due to inertia (which contributes to our feeling of dizziness when we stop rotating).
IMAGE 33.4.
Semicircular
canal cupula and
hair cells. The
hair cells of the
semicircular
canals are
located with the
ampulla of the
semicircular
canals. A
gelatinous cupula
covers the hair
cell stereocilia.
When the head
rotates, the
endolymph
within the
semicircular
canals pushes
the gelatinous
cupula causing it
to deflect in the
opposite
direction of head
movement.
When the cupula
moves, the hair
cell stereocilia
within the cupula
are bent, which
causes either
depolarization or
hyperpolarization
depending on
the direction of
movement.
Central Vestibular Pathways

The vestibular system is responsible for coordinating our balance through our head and body
movements. As such, the neurons of the vestibular system signal to motor neurons that adjust head,
eye, and body position.
The vestibular hair cell information is passed to the brain via excitatory synapses onto a branch of
the vestibulocochlear nerve (CN VIII). The cell bodies of these neurons are in Scarpa’s ganglion.
These axons send projections to several brain areas, notably the cerebellum, which is a structure
critically important for balance. CN VIII also projects into the vestibular nuclei of the medulla.
From there, neurons signal to areas of the thalamus that ultimately control motor areas of the face,
extraocular motor neurons that ultimately control eye movement, and the spinal cord that is
responsible for limb and neck motor movements to help maintain body and head position.
IMAGE 33.5.
Central vestibular
pathways.
Vestibular
information is
carried by Cranial
Nerve VIII (the
vestibulocochlear
nerve) to the
vestibular nuclei
within the
medulla. From
the medulla,
vestibular
information is
conveyed to
multiple
structure to
control vestibular
sensation.
Connections to
the cerebellum
are important in
maintaining
balance. Other
connections that
are routed
through the
ventral posterior
(VP) nucleus of
the thalamus is
important in
sensation and
motor activity of
the face.
Connections to
the extraocular
motor neurons
are important in
orienting the
eyes to maintain
balance. Lastly,
connections to
motor neurons
that control the
limb and neck

muscles help
maintain body
balance and our
head position.
‘Central
vestibular
pathways’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Key Takeaways
• The vestibular labyrinth is located within the inner ear and functions in our sense of
balance.
• The structures of the vestibular labyrinth are the otolith organs (the saccule and
utricle) and the three semicircular canals.
• The vestibular labyrinth structures also contain hair cells similar to the hair cells
within the auditory system. Bending the stereocilia in the direction of the tallest
stereocilium results in depolarization of the hair cell and bending in the direction of
the shortest stereocilium results in hyperpolarization.
• The vestibular labyrinth structures signal to multiple brain areas to control head
movement, eye movements, and balance.
Test Yourself
Attributions
Media Attributions
• Vestibular organs © NASA adapted by Valerie Hedges is licensed under a Public Domain license
• Otolith organ hair cells © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Hair cell depolarization and hyperpolarization © Valerie Hedges is licensed under a CC BY-NC-
• Semicircular canal cupula © NASA adapted by Valerie Hedges is licensed under a Public
Domain license
• Central Vestibular Pathway © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
GUSTATORY SYSTEM | 455
34.
GUSTATORY SYSTEM
Your gustatory system, which mediates your sense of taste,

helps you walk the line between health and illness. It acts
as a short-range detection system, as you must actually put Resources
something in your mouth to taste it.
The gustatory system guides you towards foods that are
energy rich and keeps you away from food that could
• Glossary Terms
make you sick. Humans can perceive five basic tastes: salty,
• Key Takeaways
sour, bitter, sweet, and umami. These specific taste
• Test Yourself
modalities all support this balance. Sweet foods taste good
because foods rich in sugar, such as fruit and wheat,
contain large amounts of usable energy, and humans have
evolved to find these foods appetizing. In contrast, toxic compounds are often bitter, causing you to
respond with feelings of disgust. Salty taste indicates a substance with high salt content and sour taste
indicates an acidic food, and umami taste indicates a high protein food.
Some of our taste is innate, like sweetness, whereas other tastes are learned like bitterness. This
explains why many individuals do not initially like the taste of coffee, and why many individuals prefer
to have their coffee with sugar when they first start drinking it. Our tastes can also be modified by our
dietary needs, like craving a salty food.
Organs of Taste
Although taste receptor cells are most prevalent on the tongue, there are other regions of the mouth
and throat, including the palate, pharynx, and epiglottis, that also are sensitive to food and play a role
in taste perception.
Other sensory information is also tightly linked to our sense of taste. Smell is important in our sense
456 | GUSTATORY SYSTEM
of taste as odorant compounds from food can reach odor receptors in the nasal cavity. How a food
looks (sense of vision) is also important to how it tastes. The texture and temperature of the food is
also important (somatosensory information). Even a sense of pain, related to the spicy level of the food
relates to our sense of taste.
Figure 34.1. The tongue is the primary location for taste receptors cells, but receptors are also
located along the palate, pharynx, and epiglottis. Additionally, airborne compounds from food can
reach odor receptors in the nasal cavity. The sense of smell plays an important role in the perception
of flavor. ‘Throat Anatomy’ by Casey Henley is licensed under a Creative Commons Attribution
Tongue anatomy
The surface of the tongue is covered in small, visible bumps called papillae. Taste buds are located
within the papillae, and each taste bud is made up of taste receptor cells, along with supporting cells
and basal cells, which will eventually turn into taste receptors cells.
The taste cells have a lifespan of approximately two weeks, and the basal cells replace dying taste
cells. The taste cells have microvilli that open into the taste pore where chemicals from the food can
interact with receptors on the taste cells. Although taste cells are not technically neurons, they synapse
and release neurotransmitters on afferent axons that send taste perception information to the brain.
Figure 34.2. The visible bumps on the surface of the tongues are papillae that house taste buds.
Taste buds are made up of taste cells and basal cells. The taste cells synapse on afferent axons that
send information to the central nervous system. Tastants in food access the taste cells via the taste
pore, where the food particles interact with the microvilli of the taste cells. ‘Tongue Anatomy’ by
The entire tongue is capable of perceiving all five tastes, meaning there are taste receptors for each taste
present across the entire surface. However, some regions of the tongue have a slightly lower threshold
to respond to some tastes over others. The tip of the tongue is the region most sensitive to sweet, salt,
and umami tastes. The sides are most sensitive to sour, and the back of the tongue to bitter tastes.
Figure 34.3.
Although all
tastes can be
perceived across
the entire
tongue,
sensitivity levels
vary for each
taste. The front
of the tongue
has the lowest
threshold for
sweet, salt, and
umami tastes;
the side of the
tongue has the
lowest threshold
for sour tastes,
and the back of
the tongue has
the lowest
threshold for
bitter tastes. It is
inaccurate to
map each of the
tastes to a single
region of the
tongue because
each area of the
tongue is capable
of sensing each
type of taste.
Taste transduction
Currently, it is believed that humans sense five basic tastes: Salty, sour, sweet, bitter, and umami. Salty
and sour taste are both mediated by ionotropic taste receptors, while sweet, bitter, and umami taste are
mediated by metabotropic taste receptors.
Salt
Salt taste is mediated by the presence of epithelial sodium channels. These receptors are usually open,
and when foods are ingested with high salt concentrations, sodium flows into the cell causing a
depolarization. This change in membrane potential opens voltage-gated sodium and calcium channels.
The increased calcium influx causes the release of serotonin-filled vesicles. The serotonin acts on the
afferent taste axon causing depolarization and action potentials.
Figure 34.4. When salty foods are ingested, the sodium from the food enters the taste cell via open
epithelial sodium channels. The resulting depolarization opens voltage-gated sodium and calcium
channels, leading to release of serotonin onto the afferent taste axon. ‘Salt Taste Transduction’ by
Salty foods elicit a biphasic response depending on concentration. Foods cooked with a low
concentration of salt taste bland and are not very appetizing; however, high salt concentrations elicit a
strong aversive reaction – imagine how disgusted you were when you first tried to cook and were overly
generous with the salt!
Additionally, the appeal of salt at a given moment depends on our body’s need for salt at the
time. Several hormones such as the appetite-stimulating hormone ghrelin contribute to regulating the
concentration of salt in the body by mediating Na+ absorption. Current salt levels can also impact
appetite for salt. For example, in chronically-sodium deprived animals, high salt solutions are highly
rewarding.
Why are we so sensitive to the taste of salt? As it turns out, both sodium and chloride are essential
nutrients. They are critical for maintaining blood volume and pressure, for regulating body water, for
maintaining muscle contractions, and mediating action potentials.
Sour
Foods taste sour because of their acidity, and when acids are present in water, they produce hydrogen
ions (protons). Therefore, a sour food has high acidity and a high concentration of hydrogen ions, thus
a low pH. The exact mechanism for sour taste transduction has yet to be worked out, but it is believed
that protons enter the cell through an ion channel, and then block potassium channels. The decreased
efflux of potassium, along with the presence of the protons, depolarizes the cell causing voltage-gated
sodium and calcium channels to open. Like salt taste transduction, the increase in intracellular calcium
causes release of serotonin into the synapse.
Figure 34.5. When sour foods are ingested, the protons from the acid enter the cell via open ion
channels. The protons then block potassium channels. The resulting depolarization opens
voltage-gated sodium and calcium channels, leading to release of serotonin onto the afferent taste
axon. ‘Sour Taste Transduction’ by Casey Henley is licensed under a Creative Commons Attribution
The purpose for our ability to sense acids in our food is under debate. Sour tastants do not inherently
provide any nutritional value, except in the case of Vitamin C. Humans and other higher primates
cannot synthesize Vitamin C on their own, so it’s possible that we evolved to find a combination of
sourness and sweetness attractive enough to consistently consume Vitamin C-rich fruits. However,
sour can be aversive, motivating us to avoid spoiled or unripe foods that might contain pathogens.
Bitter
Bitter, sweet, and umami compounds all activate taste receptor cells via G-protein coupled receptors.
The bitter receptors are from the T2R family of receptor proteins; humans have over 25. Each taste
cell can express most or all of the different receptor types, allowing for the detection of numerous
molecules, which is important when wanting to avoid dangerous substances like poisons and toxins.
Activation of the G-protein receptor activates phospholipase C, which in turn increases production
of the second messenger inositol triphosphate. Inositol triphosphate causes the release of calcium from
intracellular stores. Calcium causes the opening of ion channels, allowing the influx of sodium. These
ion changes depolarize the cell and cause ATP-specific channels to open, allowing ATP to flow out of
the cell and enter the synapse to act on the afferent taste axon by binding to purinergic receptors on the
postsynaptic cell.
Figure 34.6. Bitter foods activate G-protein receptors, which initiate the phospholipase C second
messenger system. IP3 releases calcium from intracellular stores, and the calcium opens ion
channels that allow sodium influx. The resulting depolarization causes ATP release onto the afferent
taste axon. ‘Bitter Taste Transduction’ by Casey Henley is licensed under a Creative Commons
Sweet
Sweet and umami receptors are comprised of G-protein coupled dimers, meaning two separate
proteins function together as one. The receptors are encoded by the T1R family of receptor proteins.
Sweet receptors are dimers of the T1R2 and T1R3 proteins. Both proteins need to be present and
functioning for activation of a sweet taste cell. Like bitter cells, activation of the G-protein receptor
uses a second messenger system to release calcium from intracellular stores and increase the influx of
sodium. These ion changes depolarize the cell and cause ATP-specific channels to open, allowing ATP
to enter the synapse and act on the afferent taste axon.
Figure 34.7. Sweet foods activate G-protein receptor dimers, which initiate the phospholipase C
second messenger system. IP3 releases calcium from intracellular stores, and the calcium opens ion
channels that allow sodium influx. The resulting depolarization causes ATP release onto the afferent
taste axon. ‘Sweet Taste Transduction’ by Casey Henley is licensed under a Creative Commons
Sugars like glucose and sucrose are essential for the survival of a species, since they are the main source
of cellular energy. Therefore, our ability to detect sweetness plays a central role in regulating how much
energy we take into our bodies. Of all the taste modalities, sweet is the strongest driver of food selection.
Umami
Umami is the taste of savory deliciousness, such as the taste of rich chicken broth, a perfect medium-
rare steak, or aged cheese. The word is derived from the Japanese word umai, which means “delicious.”
Umami is signaled when a molecule of glutamate (chemically the same as the neurotransmitter) binds
to T1R1/T1R3 receptors.
Umami receptors are comprised of the T1R3 protein, like the sweet receptor, but it is paired with
the T1R1 protein. Once the G-protein coupled receptor is activated, the transduction pathway is the
same as bitter and sweet taste cells.
Figure 34.8. Umami compounds activate G-protein receptor dimers, which initiate the
phospholipase C second messenger system. IP3 releases calcium from intracellular stores, and the
calcium opens ion channels that allow sodium influx. The resulting depolarization causes ATP
release onto the afferent taste axon. ‘Umami Taste Transduction’ by Casey Henley is licensed under
License.
Through evolution, we have come to prefer the taste of umami because glutamate is a byproduct
of cooking food. Cooking foods changes their chemical properties, thereby improving digestion,
reducing toxicity, and increasing absorption of nutrients. Glutamate is one of the byproducts in the
process of heating a food, and so it benefits us to appreciate these flavors.
Coding Properties of Taste

Of the five tastes, only two neurotransmitters are used to communicate information to the central
nervous system, so how does our brain know what tastes to perceive? The answer is how the
information is encoded. Most taste cells use a labeled line coding method, which means that each cell
and the related afferent taste axon only responds to one type of taste. For example, bitter cells only
express bitter receptors and are only activated by bitter molecules. These bitter taste cells activate bitter
sensory neurons and bitter regions of the taste cortex.
A small portion of taste cells do use population coding as well, meaning more than one tastant
can activate the cell, and perception is based on a combination of multiple cells each with a different
response. Most information, however, is encoded via labeled line at the level of the taste cell.
Figure 34.9. Labeled lined coding occurs when one sensation (in this case, a specific taste) leads to
activation of the sensory cell. In this example, Cell 1 is activated only by quinine, a bitter compound,
Cell 2 is activated only by sugar, a sweet compound, and Cell 3 is activated only by MSG, an umami
compound. Most taste cells in the tongue use labeled line coding. Population coding results from
broader activation, where multiple sensations can activate a sensory cell and perception is a result
of information from a population of cells. In the example, Cells 4 and 5 are activated by both salt and
acid compounds. ‘Taste Coding’ by Casey Henley is licensed under a Creative Commons Attribution
Pathway
The tongue is innervated by three cranial nerves. The front two-thirds of the tongue is innervated by
cranial nerve VII (Facial nerve). The back third is innervated by cranial nerve IX (Glossopharyngeal
nerve). Finally, the epiglottis and pharynx are innervated by cranial nerve X (Vagus nerve). All three
cranial nerves enter the brainstem at the medulla and synapse in the nucleus of the solitary tract. From
there, information is sent to the ventral posterior medial nucleus of the thalamus. Thalamic neurons
send projections to the gustatory cortex. The gustatory cortex is located deep in the lateral fissure in
a region called the insula. Information processing taste stays primarily on the ipsilateral side of the
nervous system that is to say, taste information from the left half of the tongue gets represented in the
left hemisphere of the brain, and visa versa.. Projections within the brain also exist between the taste
regions and the hypothalamus and amygdala.
Figure 34.10. Taste information from the tongue travels through cranial nerves VII, IX, and X to the
nucleus of the solitary tract in the medulla. Neurons in the brainstem project to the ventral posterior
medial nucleus of the thalamus and then on to the gustatory cortex. ‘Taste Pathway’ by Casey
Figure 34.11 Axons from sensory afferents from the tongue and throat travel to the nucleus of the
solitary tract in the brainstem via cranial nerves VII, IX, and X. The second-order brainstem neurons
project to the ventral posterior medial nucleus of the thalamus. The thalamic third-order neuron
projects to the primary gustatory cortex, which is located at the border of the frontal and temporal
lobes. ‘Taste Pathway’ by Casey Henley is licensed under a Creative Commons Attribution
View the facial nerve (cranial nerve VII) using the BrainFacts.org 3D Brain
View the glossopharyngeal nerve (cranial nerve IX) using the BrainFacts.org 3D Brain
View the vagus nerve (cranial nerve X) using the BrainFacts.org 3D Brain
Flavor
How do 5 basic tastes turn into the myriad complex taste sensations we experience when eating food?
Olfaction plays an important role in the perception of flavor, as do vision and touch. Taste information
combines with information from these other sensory systems in the orbitofrontal cortex located in the
frontal lobe. This region is believed to be important for the pleasant and rewarding aspects of food.
Additionally, as taste is processed in higher-order regions of the CNS, information is combined using
population coding mechanisms. Test how your senses combine to create flavor at home!
View the orbitofrontal cortex using the BrainFacts.org 3D Brain
Key Takeaways
• Taste cells express specific taste receptors and are located in taste buds within the
papillae
• Salt and sour taste cells rely on ion channels to depolarize the cell and release
serotonin
• Bitter, sweet, and umami taste cells rely on G-protein coupled receptors and second
messengers that open ATP channels
• At the level of the taste receptor cells, taste is perceived by using labeled line coding
• Multiple regions in the mouth and throat play a role in processing of taste
• Three cranial nerves innervate the tongue and throat
• The cranial nerves synapse in the nucleus of the solitary tract in the medulla.
Information then travels to the ventral posterior medial nucleus of the thalamus and
then to the gustatory cortex

• To perceive complex flavors, information from other sensory systems is combined
with taste information in the orbitofrontal cortex
Test Yourself!
Attributions
Media Attributions
• Maps of tastes on the tongue © Casey Henley adapted by Valerie Hedges is licensed under a CC
• Posterior Parietal © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
470 | OLFACTORY SYSTEM
35.
OLFACTORY SYSTEM
Olfaction is the ability to sense and perceive volatile

chemicals that are suspended in the air. The typical
human can distinguish up to 10,000 distinct odors,
Resources ranging from the sweet aroma of esters produced by apples
and oranges, to the putrid smells of sulfurous compounds
produced by skunks and rotten eggs. Because odors can
drift along in the air, some chemicals can be detected long
• Glossary Terms before the source is within eyesight: think about smelling
• Scientist links to learn a burning bonfire from miles away.
more Smells affect our conscious behavior. They can
• Key Takeaways motivate us to approach freshly-baked bread or avoid a
• Test Yourself rotting animal carcass. These chemicals serve as survival
cues: bread gives us energy-rich carbohydrates while a
decaying carcass can expose us to disease.
Smell is often used in communication between animals,
especially in reproductive behaviors, territory marking and identification of individuals. Olfaction is
one of the oldest functions we possess as animals. While our sense of smell has seemed to take a backseat
to other senses relative to other animals (about 2% of the total mouse brain mediates smell, while only
a scant 0.01% of the volume of the human brain is dedicated to this function), scientists now appreciate
that our olfactory system is simply more specialized. For example, humans use the smells of sweat to
clue us into the emotional state of others, and we can even subconsciously detect sickness through
body odor.
OLFACTORY SYSTEM | 471
Anatomy of the Olfactory System

Your sensation of smell begins when odorant molecules travel through your nostrils and pass through
the nasal cavity, an empty, air-filled space just behind the front of the skull.
Figure 35.1.
Olfactory system
structures.
Odorants enter
through the
nostrils into the
nasal cavity,
which is a large
air-filled
chamber. The
olfactory
epithelium is
located at the
dorsal most
portion of the
nasal cavity. This
is where the
odorants interact
with olfactory
receptor neurons
(labeled as
olfactory nerves
in this image).
The odorants dissolve in the mucus covered dorsal-most portion of the nasal cavity, an area called
the olfactory epithelium. Embedded within the olfactory epithelium are olfactory receptor neurons,
supporting cells, and basal cells.
The axons of the olfactory receptor neurons travel through a sheet of bone called the cribiform
plate before going to the olfactory bulb. Collectively, the axons of the olfactory receptor neurons
make up Cranial Nerve 1 (Olfactory Nerve).
Also found within the olfactory epithelium and surrounding the olfactory receptor neurons are
supporting cells and basal cells. The supporting cells help dispose of dead and dying cells, metabolize
pollutants, and may also help to physically maintain the epithelium by producing mucus. The basal
cells function to replace the olfactory receptor neurons.
Figure 35.2.
Olfactory
receptor neurons
in the olfactory
epithelium.
Olfactory
receptor neurons
are embedded
within the
olfactory
epithelium. In
addition to the
olfactory
receptor neurons,
there are also
basal cells and
supporting cells.
The axons of the
olfactory
receptor neurons
exit through
holes within the
cribiform plate
and then
synapse within
the olfactory
bulb where they
synapse onto the
secondary cells
(mitral cells).
‘Olfactory
receptor neurons
and olfactory
epithelium’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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International
License.
Olfactory Receptor Neurons

The olfactory receptor neurons begin processing smell. They serve as the sensory neurons for the
olfactory system. Olfactory receptor neurons are bipolar neurons, that have a single dendrite with
many cilia that are exposed to the epithelial surface. The cilia stretch into the mucus of the olfactory
epithelium and act to increase the surface area of the neurons, providing increased space for odorants
to interact with the olfactory receptor neurons. When the odorants dissolve in the mucus they interact
with odorant receptor proteins on the cilia of the olfactory receptor neuron.
Figure 35.3.
Structure of
olfactory
receptor neuron.
Olfactory
receptor neurons
are bipolar and
have a single
dendrite that
extends into the
olfactory
epithelium.
Projecting from
the dendrite are
cilia that spread
out into the
olfactory
epithelium to
increase the
surface area of
the cell that can
interact with
odorants. The
odorants bind to
odorant receptor
proteins on the
cilia of the cells.
‘Structure of
olfactory
receptor neuron’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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License.
Their proximity to the air make them the only neurons that are directly exposed to the outside world.
Unfortunately, this causes them to encounter all sorts of dangers such as toxins, particulates, and
microbes. They are one of the few known populations of neurons where adult neurogenesis regularly
occurs, each having a lifespan in the range of 30 days to a year. The average human olfactory system has
somewhere between 6-20 million olfactory receptor neurons.
Olfactory Transduction
Though taste uses different signaling systems for transduction of each of the different tastes, olfaction
uses a single system for transduction. First, odorants bind to odorant receptor proteins (a G-protein-
coupled receptor) on the cilia of the olfactory receptor neurons.
Odorant Receptor Proteins

It is estimated there are about 1,000 different genes (about 3% of the total human genome) that code
for roughly 400 different odorant receptor proteins. Each olfactory receptor neuron expresses only
one type of odorant receptor protein. The initial research into the genetics underlying these neurons
earned Drs. Linda Buck and Richard Axel a Nobel prize in 2004.
Odorant receptor proteins are transmembrane G protein-coupled receptors that have extracellular
binding sites on their surface that bind to odorants. Each receptor differs in their specific binding
pockets. Importantly, the receptor does not bind to one specific odorant, but rather binds to a specific
molecular feature of the odorant. This means that many odorants can activate more than one odorant
receptor protein, ultimately allowing each odorant to generate a unique pattern of activity across the
olfactory receptor neurons.
Thus, analyzing the activity of a single olfactory receptor neuron is not sufficient to understand how
that odorant alters neural signaling. Rather, the olfactory system uses population coding.
Figure 35.4.
Odorant receptor
proteins.
Odorants are
shown as the
blue shapes on
the left of the
image. Each
odorant has
different features
that allows for it
to act with
different odorant
receptor proteins.
The odorant
receptor proteins
are shown in red
if the odorant
can bind to that
odorant receptor
protein. The
binding pocket
within the
odorant receptor
protein does not
need to match
the entire shape
of the odorant
molecule, but
rather just one
structural feature
of the odorant. In
the top row of
image, the
odorant can bind
to the two
left-most
odorant receptor
proteins.
Odorant Receptor Protein Example
In this example we are looking at three different olfactory receptor neurons that express
different types of odorant receptor proteins labeled Cell 1 (‘blue’ odorant receptor), Cell 2
(‘orange’ odorant receptor), and Cell 3 (‘purple’ odorant receptor). These neurons are
exposed to four different odorants (vanilla, lavender, mint, and rose), and their neural
activity is measured via an electrode.
When looking at the response to the ‘Vanilla” odor, cell 1 shows an increase in the rate of
action potentials, cell 2 shows very little activity, and cell 3 shows an increase in action
potentials. Whereas the ‘Lavender’ odor only causes a response in cell 2. The ‘Mint’ odor
causes very little activity in cell 1, and increased activity in both cell 2 and cell 3. Lastly, the
‘Rose’ odor causes an increase in activity in cell 1 and cell 2, but very little activity in cell 3.
These recordings demonstrate that a single odorant can cause changes in activity in more
than one type of olfactory receptor neuron, due to the receptor proteins recognizing more
than one type of molecular feature of the odorant molecules.
Figure 35.5.
Example of
population coding
for olfactory
receptor neurons.
Three different
cells are labeled
within the
olfactory
epithelium (cell 1-
blue, cell 2,-
orange, and cell
3- purple).
Recordings of
action potentials
for each of the
three cells are
shown when
presented with
different
odorants (vanilla,
lavender, mint,
and rose). Each of
the cells responds
to more than one
different odorant.
Some cells
respond
preferentially to a
single odorant.
For example cell 2
preferentially
responds to
lavender odors.
Odorant receptor
proteins use
population coding
to code for
different
odorants. This
means that the
activity of a
population of
cells must be
measured to
determined how
an odorant
ultimately alters
neuronal
signaling.
‘Population
coding for
olfactory receptor
neurons’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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License.
Transduction Pathway
After the odorant binds to the odorant receptor protein, the receptor activates an associated G protein
called Golf. This protein complex is 90% similar to the stimulatory G protein Gs , and likewise triggers
activation of adenylyl cyclase, elevating the intracellular concentration of cyclic AMP. cAMP binds to
cyclic nucleotide-gated cation channels that when opened, allow for the influx of calcium and sodium.
The influx of calcium opens calcium-activated chloride channels. Olfactory receptor neurons have
high intracellular chloride concentration, so the opening of the chloride channel results in chloride
flowing out of the cell. Together, this causes the olfactory receptor neuron to depolarize, leading
to membrane depolarization within the dendrite. This change in membrane potential is called a
“receptor potential”. If depolarization crosses threshold potential within the olfactory receptor
neuron cell body, then the olfactory receptor neuron will fire action potentials down its axons that
make up the olfactory nerve toward the olfactory bulb.
Figure 35.6.
Olfactory
receptor neuron
transduction.
Odorant
molecules binds
to odorant
receptor proteins
located on the
cilia of olfactory
receptor neurons.
Odorant receptor
proteins are
G-protein
coupled
receptors that
are coupled to
Golf. Similar to
activation of Gαs,
activation of Golf
increases activity
of adenylyl
cyclase, which in
turn increases
cAMP production
within the cell.
cAMP causes the
opening of cation
channels in the
cell membrane
that allow for the
influx of both
calcium (Ca2+)
and sodium
(Na+). The
calcium influx
opens chloride
channels.
Chloride
concentration is
high within the
olfactory
receptor neurons,
so chloride flows
out of the cell.
The increase in
positively
charged ions
combined with
the loss of
negatively
charged ions
from inside the
cell leads to
membrane
depolarization.
‘Olfactory
receptor neuron
transduction’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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License.
Olfactory receptor neurons encode the intensity of smells through the frequency of action potential
firing, which changes in accordance with the concentration of odorant molecules. Imagine standing
over a fresh-baked pizza and inhaling deeply. Due to the high concentration of odorant molecules in
the air, several receptors will be activated, leading to frequent neuronal firing. Now, imagine you are
down the block from a pizza restaurant, getting only a slight whiff of those same scents. Here, the
concentration of odorants is low, meaning that the Olfactory receptor neurons fire less frequently.
Termination of Olfactory Signaling

Because odorants are found in the air, they are highly mobile and can easily diffuse away, no longer
having the ability to activate odorant receptor proteins. Olfactory signaling can also be terminated
through the activity of enzymes located within the mucus of the olfactory epithelium.
Our perception of smells can also fade, even if the odorant is still present in the air. The odorant
receptor neurons can adapt to the presence of an odorant, and thus no longer signal its presence.
Central Olfactory Pathway

The axons of the olfactory receptor neurons pass through the skull through a tiny series of holes at
the cribriform plate. These primary neurons form synaptic connections onto neurons in the olfactory
bulb called mitral cells, forming the beginning of the olfactory nerve (CN I). Like the optic nerve, the
olfactory nerve runs along the ventral surface of the brain.
Figure 35.7.
Human olfactory
bulbs and tracts.
In this ventral
view of the brain,
the optic bulbs
and tracts
(colored pink) run
along the ventral
surface of the
brain.
Glomeruli within the Olfactory Bulb

The site of synaptic connectivity between the olfactory receptor neurons and the secondary neurons
in the olfactory bulb (mitral cells) is a highly specialized clump of tissue called a glomerulus. The
typical human has a little under 2,000 glomeruli, and each glomerulus only receives inputs from
olfactory receptor neurons that express the same type of odorant receptor proteins. This means that
even through the olfactory receptor neurons that express the same type of odorant receptor protein
are spread out throughout the olfactory epithelium, the axons of those neurons all converge within the
same glomerulus.
Figure 35.8.
Olfactory
glomeruli.
Olfactory
receptor neurons
that contain the
same type of
odorant receptor
protein are
spread
throughout the
olfactory
epithelium. All
neurons that
contain the same
odorant receptor
protein all
synapse onto a
common
olfactory
glomerulus
within the
olfactory bulb.
Within the
olfactory
glomerulus is a
synapse onto the
secondary mitral
cells. The axons
of the mitral cells
make up the
olfactory tract
that will carry
the olfactory
signal into the
brain. ‘Olfactory
glomeruli’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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License.
Brain Targets of Olfactory Neurons

Axons leave the olfactory bulb via the olfactory tracts and project to two different targets.
First, some cells project to the olfactory tubercle, are routed through the medial dorsal nucleus
of the thalamus and then to the orbitofrontal cortex. This pathway is responsible for our conscious
perception of smell.
Other cells project directly to the olfactory cortex and other related structures found within the
temporal lobes, like the hippocampus. This makes the olfactory system the only sensory system
that does not have to first pass signals through the thalamus before cortical processing. This pathway
may play roles in discriminating odors, and the emotional, motivational, and memory-related aspects
of smell.
Figure 35.9.
Projection of
olfactory
information.
Olfactory
receptor neurons
send information
to the olfactory
bulb. From the
olfactory bulb
information can
be routed
through the
olfactory
tubercle, then to
the medial dorsal
nucleus of the
thalamus, and
finally the
orbitofrontal
cortex for
processing of
olfactory
information.
Alternatively,
olfactory
information can
leave the
olfactory bulb
and be routed
directly to the
olfactory cortex
and other related
temporal
structures like
the
hippocampus.
This makes the
olfactory system
the only sensory
system that does
not necessarily
need to be
routed through
the thalamus
prior to cortical
processing.
‘Projection of
olfactory
information’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Disorders of the Olfactory System
Like other sensory systems, the structures involved in olfaction can be injured. An injury to
the olfactory system can result in hyposmia, a reduced ability to smell, or anosmia, a
complete loss of smell.
The most common insult to the olfactory system is simple nasal congestion, a temporary,
physical blockage of the entry to the nasal cavity that decreases airflow and, therefore, the
number of particles that reach the olfactory epithelium. Congestion can be caused by
allergies, the common cold, upper respiratory bacterial or viral infections, or sinus infections.
Hyposmia is also one of the main neurological symptoms of COVID-19. Hyposmia is

common among healthy, older adults, affecting about half of the population between 65
and 80 years old. As a person ages, spontaneous calcification causes the holes in the
cribriform plate to shrink, which can impinge on and damage olfactory receptor neuron
axons. Hyposmia can also be caused by abrupt head injuries. The olfactory receptor neuron
axons that project through the holes of the cribriform plate are particularly sensitive to
blows to the head. Neurodegenerative disorders, such as Parkinson’s disease and
Alzheimer’s disease, contribute to smell deficiency. Usually, hyposmia precedes the major
clinically observed symptoms of these disorders, hinting that smell deficiency may serve as
an early diagnostic biomarker.
Another olfactory deficit, phantosmia, is when a person perceives “phantom” scents, or in

other words, experiences an olfactory hallucination. Phantosmia may be triggered by a
temporal lobe seizure or a stroke. It can also be caused by a brain tumor affecting the
olfactory nerve (CN I), or the subsequent surgical removal of the tumor, leading to injury.
Schizophrenia, a psychiatric condition characterized by auditory hallucinations, may also
cause phantosmia.
Key Takeaways
• Odorants dissolve in the mucus of the olfactory epithelium and bind to odorant
receptor proteins on olfactory receptor neurons.
• A single odorant can bind to multiple different odorant receptor proteins because the
odorant receptor protein binds to a molecular feature of the odorant.
• Odorant receptor proteins are G protein-coupled receptors that cause accumulation
of cAMP within the cell, and ultimately membrane depolarization of the olfactory
receptor neuron.
• Olfactory information is carried from the olfactory receptor neuron axons through
the cribiform plate and into the olfactory bulb where it synapses with the olfactory
glomeruli. From there, the information travels through the olfactory tracts and can
either be routed through the thalamus and then to olfactory brain areas, or can be
directly routed to olfactory cortical areas, bypassing the thalamus.
Test Yourself!
Attributions
Media Attributions
• Olfactory system © Patrick J. Lynch is licensed under a CC BY (Attribution) license

• Close up of olfactory receptor neuron © Valerie Hedges is licensed under a CC BY-NC-SA
• olfactory receptor neuron © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• odorant proteins © Joanna Kośmider adapted by Valerie Hedges is licensed under a Public
Domain license
• odoran receptor example © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Olfactory transduction © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

• Glomerulus © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Olfactory pathway © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
490 | SOMATOSENSORY SYSTEMS
36.
SOMATOSENSORY SYSTEMS
Somatosensory Cell Bodies

Resources
All somatosensory receptor neurons have their cell bodies
located in the dorsal root ganglion, a structure found
just outside the dorsal aspect of the spinal cord. The
• Glossary Terms receptor neurons, also called primary afferent fibers, of
• Key Takeaways the somatosensory system are bipolar neurons, meaning
• Test Yourself they have one process from the cell body that splits into
two branches. One travels to the location of the receptor
(e.g. the skin for touch) via the spinal nerves, and one
travels into the spinal cord at the dorsal horn via the
dorsal root. The axon can either synapse in the spinal cord or ascend to the brain in the dorsal
column.
SOMATOSENSORY SYSTEMS | 491
Figure 36.1.
Primary afferent
fibers travel from
the periphery or
target organs
through the
spinal nerve to
the dorsal root
ganglion where
the cell body of
the neuron is
located. The
axons then
continue through
the dorsal root
into the dorsal
horn of the spinal
cord. Axons can
branch and
synapse in the
spinal cord, or
they can ascend
to the brain via
the dorsal
column.
‘Somatosensory
Spinal Cord’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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License.
Primary Afferent Axons

Primary afferent axons are divided into four groups based on size and conduction speed. The groups,
unfortunately, have different names depending on if the axons come from the skin (Aα, Aβ, Aδ, and
C fibers; examples are touch or pain) or the muscles (Group I, II, III and IV fibers; example is
proprioception). The fastest axons are the Aα or Group I type; they have the largest diameter and are
heavily myelinated. The next fastest myelinated axons are the Aβ or Group II fibers, followed by the
Aδ or Group III fibers. Finally, the C fibers have the smallest diameter, are unmyelinated, and are the
slowest at conducting action potentials.
Table 36.1. Diameter and conduction speed of primary afferent axons. Group I and A alpha have the
largest diameter and fastest speed. Group II and A beta are the next largest and fastest, followed by
Group III and A delta. Group IV and C fibers are the slowest and smallest of all axon types.
Afferent Axon from Afferent Axon from Conduction Speed (m/

Diameter (μm)
Muscle Skin sec)
Group I Aα 13- 20 (Largest) 8- 120 (Fastest)
Group II Aβ 6- 12 35- 75
Group III Aδ 1- 5 5-30
Group IV C 0.2- 1.5 (Smallest) 0.5- 2 (Slowest)
Different sensory information is sent via the different types of axons. Proprioceptive information
from the skeletal muscles is sent to the spinal cord via Group I fibers. Touch information from the
mechanoreceptors travels along Aβ fibers. Aδ fibers carry pain and temperature sensation, and C fibers
convey information about pain, temperature, itch, and chemoreception.
Figure 36.2.
Primary afferent
fibers differ in
diameter and
myelination, and
therefore have
different
conduction
speeds. A alpha
fibers convey
proprioception
and are the
largest and
fastest of the
axon types.
Mechanoreceptio
n, or touch, is
sent via A beta
fibers, the next
largest. Some
aspects of pain
and
temperatures are
sent by A delta
fibers, which
have small
diameter and
little myelination.
C fibers are
unmyelinated
and sensory
axons that detect
pain,
temperature, itch
and
chemoreception
(chemical
composition).
These are the
slowest of the
somatosensory
axons.
‘Somatosensory
Axon Types’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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License.
Dermatomes
The afferent axons from the dorsal root ganglion enter the spinal cord via the spinal nerves. Axons
from nearby regions of the body enter the spinal cord together, and this forms regions of skin that are
innervated by the same spinal nerve. These regions are called dermatomes. Damage to a spinal nerve
will cause dysfunction along the innervated dermatome. The dermatomes and spinal nerves are
divided into 4 groups. The seven cervical spinal segments are the most rostral and are located in the
neck. The twelve thoracic spinal segments are located along the chest and abdomen. The five lumbar
segments are located below the thoracic segments, and the five sacral segments are the most caudal.
Figure 36.3.
Spinal nerves exit
the spinal cord
and innervate a
region of skin
called a
dermatome.
There are five
cervical, twelve
thoracic, five
lumbar, and five
sacral spinal
segments.
‘Dermatomes’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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Key Takeaways
• Somatosensory neuron cell bodies are located in the dorsal root ganglion
• Somatosensory primary afferent axons ascend to the brainstem via the dorsal
column white matter tract
• Primary afferent axons vary in diameter and myelination, both of which affect action
potential speed
• Different somatosensory information is carried by the different sizes afferents
• Dermatomes are the region of skin innervated by one spinal nerve
Test Yourself!
Attributions
Media Attributions
• Somatosensory Spinal Cord © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• SomatosensoryAxonTypes © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Dermatomes © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
TOUCH: THE SKIN | 497
37.
TOUCH: THE SKIN
Touch can come in many forms: pressure, vibration,

stretch, motion, edges, points, etc. Receptors in the skin
allow for perception of these different characteristics, and Resources
when this information is combined in the central nervous
system, we are able to determine the location, strength,
duration, movement, shape, and texture of the object
• Glossary terms
interacting with the skin.
• Key Takeaways
Cutaneous (meaning found in the skin)
• Test Yourself
mechanoreceptors are responsible for sensing
mechanical changes to the skin.
Receptors
We can feel different modalities of touch because of the presence of specialized sensory receptors, called
mechanoreceptors, located in the skin.
There are four different classes of cutaneous mechanoreceptors. These different mechanoreceptors
have slightly different shapes and are therefore capable of detecting different types of stimuli.
1. Merkel’s discs are located at the border between the dermis and epidermis and are specialized to
detect edges and points. They are very densely populated underneath our fingertips. These are
the most precise mechanoreceptors with resolution at the level of 0.5 mm, which allow us to
detect different distances in bumps while running your fingers across Braille.
2. Pacinian corpuscles are mechanoreceptors wrapped in several layers of connective tissue. The
Pacinian corpuscles are located deep in the dermis of the skin and are responsible for perception
of vibrations.
498 | TOUCH: THE SKIN
3. Meissner’s corpuscles are located in the epidermis layer and are highly concentrated at the
fingertips. They are stimulated by skin motion/movement.
4. Ruffini endings are located in the dermis layer of the skin and respond to stretching of the skin.
Figure 37.1. The

different
mechanoreceptor
types are located
in different
regions of the
skin and are
responsible for
perception of
different
characteristics of
a touch timulus.
Pacinian
corpuscles and
Ruffini endings
are located deep
in the dermis.
Meissner
corpuscles are
located in the
dermis near the
epidermis, and
Merkel cells are
located in the
epidermis, near
the surface of
the skin.
‘Mechanorecepto
rs’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Structure of a Cutaneous Mechanoreceptor

The somatosensory afferents from the cutaneous mechanoreceptor have their cell bodies located in the
dorsal root ganglion (DRG), a clump of somata that are close to the dorsal side of the spinal cord.
These touch receptors are the primary neurons and are classified as pseudounipolar neurons based on
their morphology. Pseudounipolar neurons don’t have true dendrites; instead each soma sends a single
protrusion which branches into two directions. One branch goes towards the skin surface where it
terminates as one of the mechanoreceptors. Typically, the terminal of this branch is surrounded by a
connective tissue covering. The other branch off the soma travels towards the spinal cord.
Figure 37.2.
Cutaneous
mechanoreceptor
structure.
Cutaneous
mechanoreceptor
s have their cell
bodies located
within the dorsal
root ganglion.
These
pseudounipolar
neurons have a
singular
projection from
the cell body that
branches, with
one branch
traveling out to
the skin. The
axon terminal is
surrounded by a
layered
connective tissue
covering
(capsule). The
other branch
travels to the
spinal cord.
‘Cutaneous
mechanoreceptor
structure’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
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License.
Sensory Transduction
On a molecular scale, these mechanoreceptors detect changes at the skin using mechanically-gated
ion channels. In previous chapters we discussed ion channels that are gated by voltage changes and
channels that are gated by neurotransmitters. In the somatosensory system, we find ion channels
that are gated by physical distortion or stretch of the membrane. These transmembrane proteins
are specialized for detecting the physical distortion of the channel and can open by stretch of the
membrane itself or indirectly through movement of intra- or extracellular proteins that are linked to
the channels. They are specialized for detecting the physical distortion of the channel, similar to the
channels found in the stereocilia of the hair cells or vestibular cells.
When pressure is applied to these proteins, the cation channel opens and sodium and calcium
flow down their electrochemical gradient into the neuron, causing both a depolarization and the
initiation of second messenger cascades. This depolarization is referred to as a ‘receptor potential’. If
enough stimulus is applied, the depolarization reaches threshold potential for the cell and an action
potential is sent toward the spinal cord.
Animation 37.1 Mechanoreceptors respond to touch stimuli via stretch-gated non-selective cation
channels. The channels can either open due to stretch of the membrane itself which stretches open
the channel or due to proteins associated with the channels that pull the channel open. ‘Stretch-Gated
Ion Channels’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Receptive Fields
Each cutaneous mechanoreceptor is able to detect somatosensory information in an area of skin called
the receptive field. When the receptive field is touched, the mechanoreceptor will be activated. The
size of a receptive field can be determined by measuring the rate of action potentials from the nerve that
collects somatosensory information from that patch of skin. The smaller the receptive field, the better
the brain can distinguish between two different adjacent tactile stimuli.
Figure 37.3. Each

mechanoreceptor
will be activated
by a specific
region of skin,
the receptive
field. When no
stimulation of
the receptive
field occurs on
the surface of
the skin, the
mechanoreceptor
will show a
baseline firing
rate. When
stimulation of
the receptive
field occurs, the
firing rate of the
mechanoreceptor
will increase.
‘Receptive Field
Activation’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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License.
Receptive Field Size

Additionally, different classes of mechanoreceptors have different sizes of receptive fields: deeper
structures, like Pacinian corpuscles, have larger receptive fields compared to receptors found closer
to the skin surface that have smaller receptive fields. Merkel cells and Meissner corpuscles, both of
which are located near the skin surface, have small receptive fields and thus collect information from
a restricted area of sensory space. Ruffini endings and Pacinian corpuscles, located deeper in the
skin layers, have larger receptive fields than the Merkel cells and Meissner corpuscles and thus collect
information over a large are of sensory space.
Figure 37.4.
Receptive field
sizes vary
depending on
the underlying
mechanoreceptor
type and
location. Merkel
cells and
Meissner
corpuscles have
small receptive
fields, whereas
Pacinian
corpuscles and
Ruffini endings
have large
receptive fields.
‘Mechanorecepto
r Receptive
Fields’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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Receptive field sizes are different among the different mechanoreceptors, but they also vary among
different body regions. Even within one receptor type (e.g. Meissner corpuscles), receptive fields in
regions like the fingers or lips are smaller than in regions like the back or leg. This allows us to have finer
spatial resolution with locating and identifying objects using our fingers. The smaller receptive fields in
these regions are a result of a higher density of receptors in the skin.
Figure 37.5.
Density of
mechanoreceptor
s can affect the
size of the
receptive field for
each receptor.
High density
leads to smaller
receptive fields.
Density and
receptive field
size varies by
location on the
body. Regions
like the hands
and face have
smaller receptive
fields than
regions like the
back. ‘Receptive
Field Location’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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License.
Two-Point Discrimination
Receptive field sizes are important because they allow us to locate a stimulus on our bodies. Larger
receptive fields are not as precise as smaller receptive fields. One measure of receptive field size is two-
point discrimination (try it at home!), which determines the minimum distance needed between two
stimuli to perceive two separate points on the skin and not one. The hand has a smaller threshold for
discerning between two points than does the back, a result of the different sized in receptive fields. In
skin areas with a large receptive field, two adjacent stimuli may feel like one.
Figure 37.6. The

size of the
receptive fields
affect the
sensitivity of the
skin, which can
be measured by
the two-point
discrimination
test. Tools like
calipers or even a
paperclip can be
used to measure
two-point
discrimination. If
the two points of
the caliper feel
like one point,
they are both
activating the
same receptive
field, indicating
the receptive
field is large. If,
however, it is
possible to
perceive two
separate points
on the skin, then
the calipers are
activating two
different
receptive fields.
‘Two-Point
Discrimination’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Skin areas with small receptive fields are better able to distinguish two similarly-spaced points. The
hand has a smaller threshold for discerning between two points than does the back, a result of the
different sized in receptive fields.
The two-point discrimination threshold for the fingers is very small, which indicates that they
have high sensitivity. Body areas with high sensitivity typically have higher density of
mechanoreceptors, have receptors that have smaller receptive fields, and have more brain tissue devoted
to processing the sensory information that comes from those structures.
The two-point discrimination threshold for areas such as the leg or torso is very large, which
indicates that these areas have low sensitivity. Body areas with low sensitive have a lower density of
mechanoreceptors, have receptors that have larger receptive fields, and have less brain tissue devoted to
sensory information that comes from those structures.
Figure 37.7.
2-point
discrimination
thresholds across
the body. 2-point
discrimination
threshold is the
minimum
distance
between two
points, typically
measured in
millimeters,
needed to
distinguish
stimuli. The
graph depicts the
typical threshold
to distinguish
two points across
various areas of
the body. Areas
such as the legs
and stomach
have high 2-point
discrimination
thresholds,
indicating that
they are less
sensitive and
that the two
stimuli need to
be placed further
apart to
distinguish two
separate stimuli.
Areas of the
body like the
hands and
fingers have
lowers 2-point
discrimination
thresholds,
indicating that
they are more
sensitive and are

capable to
distinguishing
two separate
stimuli, even
when very close
in distance.
‘2-point
discrimination
across the body’
by Valerie
Hedges is
licensed under a
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Commons
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ommercial-Share
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License.
Adaptation Rate
Another important characteristic of the somatic sensory receptors is that of adaptation rate. The class
of mechanoreceptors can be divided into two categories based on their electrical properties in response
to stimuli: slowly adapting and rapidly-adapting.
Slowly-adapting mechanoreceptors change their action potential firing rate as long as the
stimulus is present, while rapidly-adapting mechanoreceptors only change activity at the moment
there is a change in stimulus. For example, imagine the stimulus of a coin that is sitting in the palm
of your hand. The slowly-adapting sensory neurons may increase their firing rate as long as the coin is
in your palm. When the quarter is removed, they return to their baseline firing rate. Slowly-adapting
mechanoreceptors change their action potential firing rate as long as the stimulus is present and are
most useful for determining the pressure and shape of a stimulus. Merkel cells and Ruffini endings are
both slowly adapting fibers.
Animation 37.2. Slowly adapting mechanoreceptors continuing firing action potentials throughout
the duration of a stimulus. As the stimulus moves from not present, to weak, to strong, the action
potential firing of the Ruffini ending fires throughout the entire stimulus. ‘Slowly Adapting Receptor’
Rapidly-adapting mechanoreceptors only change activity at the moment there is a change in
stimulus, but not when a stimulus is constant. This firing makes rapidly adapting fibers specialized
for detecting movement and vibration. Considering the coin example from above, a rapidly adapting
sensory neuron will change its action potential firing properties only at two timepoints: the moment
the coin lands in your hand, and the moment that coin is removed. These neurons signal a change in
status.
Animation 37.3 Rapidly adapting mechanoreceptors firing action potentials when the strength of the
stimulus changes. As the stimulus moves from not present, to weak, to strong, the action potential
firing of the Pacinian corpuscle only fires when the stimulus changes strength. ‘Rapidly Adapting
Receptor’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Key Takeaways
• There are multiple types of mechanoreceptors in the skin that are activated by
different types of touch stimuli
• The receptive field size differs among the types of mechanoreceptors
• The adaptation rate differs among the types of mechanoreceptors
• Receptive field is a region of skin that activate a given mechanoreceptor
• Receptive field size for a specific type of mechanoreceptor can vary in size across the
body
• Mechanoreceptors express stretch-gated non-selective ion channels that depolarize
the cell during sensory transduction
Test Yourself!
Additional Review
Describe the relationship between density of receptors, receptive fields, and two-point
discrimination.
Answers
Attributions
Media Attributions
• Mechanoreceptors © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• somatosensory afferent © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Receptive Field Activation © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Mechanoreceptor Receptive Fields © Casey Henley is licensed under a CC BY-NC-SA
• Receptive Field Location © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Two Point Discrimination © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• 2 point discrimination © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
TOUCH: CENTRAL PROCESSING | 513
38.
TOUCH: CENTRAL PROCESSING
Receptive Fields and

Lateral Inhibition Resources
The receptive fields of the sensory neurons become more

complex as information moves up the pathway. We saw in
the last lesson that mechanoreceptors have receptive fields • Glossary terms
that, when touched, activate the neuron. The
more!
mechanoreceptors synapse on neurons in the dorsal
• Key Takeaways
column, and those neurons have more complex receptive
• Test Yourself
fields. The dorsal column nuclei have receptive fields that
are divided into center and surround regions. The center
of the receptive field is a result of direct innervation from
the mechanoreceptors. If a stimulus touches the skin in
the center of a dorsal column neuron’s receptive field, the neuron will increase its firing rate. The
center / surround structure is like that of bipolar and ganglion cells in the vision system.
514 | TOUCH: CENTRAL PROCESSING
Figure 38.1. The

receptive field of
a dorsal column
neuron has an
excitatory center
that is generated
by the
mechanoreceptor
s that synapse
directly on the
dorsal column
neuron. A) When
no stimulus is
present, the
dorsal column
neuron fires at a
baseline rate. B)
When a stimulus
touches the
center of the
receptive field of
Cell E, the firing
rate increases.
‘Touch Receptive
Field Center’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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The surround region of the receptive field is a result of indirect communication between the receptor
neurons and the dorsal column neurons via inhibitory interneurons. The surround has an inhibitory
effect on the dorsal column neuron. If a stimulus touches the skin in the surround of a dorsal column
neuron’s receptive field, the neuron will decrease its firing rate.
Figure 38.2. The

receptive field of
a dorsal column
nucleus has an
inhibitory
surround, which
is a result of the
indirect
connections
between
mechanoreceptor
s and the dorsal
column neuron
via inhibitory
interneurons. A)
When no
stimulus is
present, the
dorsal column
neurons fire at a
baseline rate. B)
When a stimulus
touches the
surround of the
receptive field of
Cell E, the firing
rate decreases.
Note that the
stimulus is in the
surround of Cell
E’s receptive field
but is also in the
center of Cell D,
so the firing rate
of Cell D will
increase. ‘Touch
Receptive Field
Surround’ by
Casey Henley is
licensed under a
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Lateral Inhibition
The center-surround structure of the receptive field is critical for lateral inhibition to occur. Lateral
inhibition is the ability of the sensory systems to enhance the perception of edges of stimuli. At a
point or an edge of a stimulus, because of the inhibitory interneurons, the perceived stimulus strength
will be enhanced compared to the actual stimulus strength.
Figure 38.3.
Lateral inhibition
heightens the
perception of
edges or points
on the skin. The
point of a blunt
probe pressing
on the receptive
field of Cell B will
cause an increase
in the firing rate
of Cell E, but will
also cause a
decrease in the
firing rate of Cells
D and F. This
increased the
perceived
difference
between the
point and the
area next to the
point that is not
being stimulated.
‘Touch Lateral
Inhibition’ by
Casey Henley is
licensed under a
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Pathway to Brain
Dorsal Column-Medial Lemniscus Pathway

The pathway from the periphery to the somatosensory cortex consists of three neurons: a first-order
sensory neuron, a second-order medulla neuron, and a third-order thalamic neuron.
Specifically, the primary afferent sensory fibers (sensory neurons) have their cell bodies located in
the dorsal root ganglion, a structure that lies just outside of the spinal cord. The axons of these first-
order neurons enter the ipsilateral dorsal side of the spinal cord. Some axon collaterals terminate in
the spinal cord and are important for reflexes.
The main axon branch ascends the spinal cord toward the brain, via the dorsal column, synapsing
in the dorsal column nuclei located in the medulla of the brainstem. The axons of sensory neurons
in the lower body remain separate from the axons of sensory neurons in the upper body throughout
the pathway. These two populations of neurons synapse in different regions of the medulla. The lower
body axons synapse in the gracile nucleus, whereas the upper body axons synapse in cuneate nucleus.
The second-order neurons within the medulla have axons that cross the midline, or decussate, and
ascend via a white matter tract called the medial lemniscus.
The medulla axons synapse in the ventral posterior lateral nucleus of the thalamus. The third-
order thalamic neurons then project to the primary somatosensory cortex located in the postcentral
gyrus in the parietal lobe.
Figure 38.4
Somatosensory
information from
the neck and
body travels
through the
dorsal column –
medial lemniscus
pathway, named
for structures
within the
pathway. Axons
enter the spinal
cord and ascend
through the
dorsal column to
the medulla
where
decussation, or
crossing the
midline, occurs.
Information
continues to the
thalamus via the
medial lemniscus,
and then reaches
the
somatosensory
cortex. Details of
the pathway are
found in the text.
‘Touch Pathway
from Body’ by
Casey Henley is
licensed under a
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Trigeminal Pathway
The pathway from the face and head to the somatosensory cortex also consists of three neurons.
Sensory receptors in the face and head send information to the brain via cranial nerve V, the trigeminal
nerve. The first-order neurons have their cell bodies in the trigeminal ganglion, located just outside
of the brainstem, and they project to the ipsilateral trigeminal nucleus in the pons. The second-
order neurons within the pons cross the midline and project up to the ventral posterior medial
nucleus of the thalamus. These third-order thalamic neurons then send projections to the face region
of the somatosensory cortex.
Figure 38.5.
Somatosensory
information from
the head and
face travels
through the
trigeminal
pathway. Axons
enter the
brainstem at the
level of the pons
and decussate
before traveling
to the thalamus
and
somatosensory
cortex. Details of
the pathway are
found in the text.
‘Touch Pathway
from Face’ by
Casey Henley is
licensed under a
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Figure 38.6. To
compare the two
pathways,
sensory
information
comes in from
the periphery.
For the body, the
peripheral axon
branch travels via
a spinal nerve to
the cell body
located in the
dorsal root
ganglion, which
sits just outside
the spinal cord.
The central axon
branch then
enters the spinal
cord and ascends
via the dorsal
column to the
dorsal column
nuclei in the
brainstem. The
second-order
neuron crosses
the midline and
then projects to
the ventral
posterior lateral
nucleus of the
thalamus via the
medial lemniscus
tract. The
thalamic
third-order
neuron projects
to the primary
somatosensory
cortex in the
parietal lobe. For
sensory
information from
the face, the

peripheral axon
branch travels to
the trigeminal
ganglion via
cranial nerve V.
The ganglion sits
outside of the
brainstem, and
the axons then
enter the
brainstem and
synapse on the
trigeminal
nucleus. The
second-order
neuron travels to
the ventral
posterior medial
nucleus of the
thalamus, and
the third-order
neuron projects
to the primary
somatosensory
cortex in the
parietal lobe.
‘Touch Pathways’
by Casey Henley
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Primary Somatosensory Cortex
Anatomy
The primary somatosensory cortex is the postcentral gyrus located in the parietal lobe. It is divided into
four regions, each with its own input and function: areas 3a, 3b, 1, and 2. The neurons within these
regions are arranged in columns, similar to other areas of the cortex.
Most touch information from mechanoreceptors inputs to region 3b, whereas most proprioceptive
information from the muscles inputs to region 3a. These regions then send and receive information
from areas 1 and 2. As processing of somatosensory information continues, the stimuli required to
activate neurons becomes more complex. For example, area 1 is involved in sensing texture, and area 2
is involved in sensing size and shape of an object. The posterior parietal cortex, an important output
region of the somatosensory cortex, lies caudal to the postcentral gyrus; areas 5 and 7 are downstream
structures that continue to process touch.
Figure 38.7. The

somatosensory
cortex, located in
the postcentral
gyrus, just
posterior to the
central sulcus, is
divided into 4
areas: 3a, 3b, 1,
and 2. The
posterior parietal
cortex, an output
region of the
somatosensory
cortex, lies just
posterior to the
postcentral gyrus
and is divided
into areas 5 and
7. ‘Postcentral
Gyrus’ by Casey
Henley is
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Somatotopic Map
The receptive fields of each higher order neuron increases in size and complexity, but even cortical
neurons are associated with a specific region of the body. The mapping of this organization was
discovered during brain surgery. In epilepsy surgery, parts of the operation are performed as the person
is awake. The famed neurosurgeon Wilder Penfield electrically stimulated a small section of the primary
somatosensory cortex (S1), then asked the person what they felt. Patients would respond that they feel
sensations in their hand when the medial S1 was activated, the little finger when a more ventral area
was activated, and so on. In repeating this process while moving slowly across the span of S1, Penfield
mapped out the organization of S1. He discovered that the sensory and motor cortices are organized
by the region of the body they represent, so neurons that respond to sensation in the fingers are located
close to the neurons that respond to sensation in the hand.
One key observation in doing this process was the volume of brain regions corresponding to
different body parts was not necessarily proportional to the volume of skin: for example, a large portion
of the cortex was dedicated to sensory information from the lips or the fingertips, while very little was
dedicated to the shoulders or trunk. This observation indicates that certain areas of the body are more
densely packed (lips, fingertips) with sensory neurons compared to others (shoulders, trunk). Regions
with high receptor density in the skin, and, therefore, fine two-point discrimination, have more cortical
space devoted to them. This means that the cortical representation of the body is not true to actual
physical proportions.
Figure 38.8. The

body is mapped
onto the
somatosensory
cortex. Regions
with high touch
sensitivity, and
therefore high
mechanoreceptor
density, have
more cortical
space dedicated
to their
processing. The
feet and legs are
represented in
the medial
superior region
of the cortex; the
face is
represented on
the lateral side of
the cortex; the
hand and fingers
fall in between.
‘Somatotopic
map’ by Casey
Henley is
licensed under a
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A homunculus is a cartoon representation of what a body would look like if actual body size was
proportional to the cortical representation. The hands and lips would be excessively large while the
torso, arms, and legs, would be relatively small. Importantly, most homunculus representations are
based on the mapping of male brains, but far less research has been done on the mapping of female
brains.
Figure 38.9.
Somatosensory
homunculus. A
cartoon
representation of
how the body
would look if the
size of the body
parts
corresponded to
the amount of
cortical space
they occupied
within the
somatosensory
cortex. The
hands, fingers,
and lips are very
large, indicating
that they are
highly sensitive
and have a large
area of cortical
space devoted to
them. Areas like
the trunk and
legs are smaller,
indicating that
they are not as
sensitive and do
not occupy as
much space
within the
somatosensory
cortex.
Higher-Level Processing of Touch Information

The primary somatosensory cortex sends projections to other parietal lobe regions for higher-level
processing of touch information.
Secondary Somatosensory Cortex

The secondary somatosensory cortex (SII) is located in the inferior parietal lobe, just above the lateral
fissure. This region, like the dorsal stream of visual processing, is responsible for object recognition,
discerning texture, shape, and size. The SII also has receptive fields that represent bilateral regions of
the body, so both hemispheres will be activated by touch on either side of the body. The SII sends
projections to the posterior parietal cortex, the premotor cortex, the amygdala, and the hippocampus.
Posterior Parietal Cortex

The posterior parietal cortex recognizes touch characteristics like orientation and movement. It is also
important for combining the touch and motor components of actions like grasping. The posterior
parietal cortex outputs to the frontal motor cortex.
Figure 38.10. The

primary
somatosensory
cortex is located
in the postcentral
gyrus. The
posterior parietal
cortex regions
important for
somatosensory
lie caudal to the
postcentral gyrus
in the superior
parietal lobe. The
secondary
somatosensory
cortex is located
dorsal to the
lateral fissure,
caudal to the
postcentral
gyrus.
‘Somatosensory
Streams” by
Casey Henley is
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View the posterior parietal cortex using the BrainFacts.org 3D Brain
Cortical Plasticity
In adulthood, the brain is plastic, meaning synaptic connections can rearrange under certain
conditions. The somatosensory cortex has been studied with regard to its plasticity. Experience-based
plasticity was initially proposed by Donald Hebb and is often referred to as Hebbian plasticity. This
theory postulates that experience can enhance or reduce the activity of synapses to ultimately alter
their function. When a presynaptic neuron repeatedly signals to a postsynaptic neuron, the synapse
will undergo plasticity, changing structure to stabilize and strengthen the function of the synapse.
Simplistically, the phrase “neurons that fire together, wire together” is often used to describe Hebbian
plasticity. Plastic changes can include altering the amount of neurotransmitter release or altering the
number of postsynaptic receptors.
Multiple principles of experience-dependent plasticity have been identified to build off Hebb’s
original idea. We will focus on five of those principles below.
1. Use it or lose it. The idea behind this principle is that neural connections that are used
consistently are maintained and strengthened. When a synapse is strengthened, it will produce
stronger EPSPs that bring the cell membrane potential closer to threshold potential. If neural
connections are not used consistently, then those synapses are removed, or lost.
2. Use it to improve it. The idea behind this principle is that connections that are consistently
used continue to improve in function.
3. Repetition of stimulation. Synaptic strength can be enhanced by repeated stimulation.
4. Intensity of stimulation. The intensity of the stimulation will alter synaptic strength.
5. Regional and functional specificity. Experience-dependent plasticity is specific to the region
and function that is receiving the experience. This is to say, providing specific experience to one
region of the brain will enhance plasticity within that one region, and will not globally enhance
plasticity across the entire brain.
Measuring Cortical Plasticity

Michael Merzenich is a researcher that has enlightened our understanding of cortical plasticity within
the somatosensory system. He used microelectrodes to stimulate and record from the somatosensory
cortex to create detailed cortical maps, similar to the homunculus that we discussed earlier. Dr.
Merzenich completed a number of experiments that demonstrated principles of experience-dependent
plasticity, some of which are highlighted below.
In one of his experiment, he demonstrated that amputation or loss of a finger, for example, will lead
to the associated cortical space to be functionally remapped by input from neighboring regions of the
hand. The cortical neurons do not die, they begin to be activated by a different region of the body.
Figure 38.11. It is
possible to map
cortical space to
regions of skin.
A) The cortical
space mapped to
each finger for an
imaginary
individual is
shown as an
example. B) If
this individual
were to lose a
finger, in this
case digit 3 or
the middle finger,
and the cortical
space was
remapped after
time had passed,
the region that
had once
responded to
touch on digit 3
would instead
respond to touch
on either digit 2
or 4. The brain
does not let that
cortical space die
or go to waste; it
rearranges
connections to
make use of all
the neurons.
Based on
Merzenich et al.,
1984. ‘Cortical
Plasticity’ by
Casey Henley is
licensed under a
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An additional experiment showed that when two adjacent fingers were sewn together so that they
moved as a unit, a new cortical map developed that was specific to the merged fingers. Thus, cortical
maps can be removed or created anew.
Figure 38.12.
Creation of
cortical maps. A)
Somatosensory
cortical maps
were measured
for each of the
fingers prior to
the experimental
manipulation. B)
When two
adjacent digits
were merged by
sewing them
together (fingers
3 and 4 in this
example), the
area at the
junction of
fingers 3 (colored
pink) and 4
(colored blue)
(labeled M, for
merge) created a
new merged map
within the
somatosensory
cortex (colored
purple). The
maps that
previously served
the individuals
digits were
retained, but
shrank as a result
of merging digits
3 and 4. Based
on Clark et al.,
1988, ‘Merged
maps’ by Valerie
Hedges is
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Likewise, cortical representation can expand with use or practice. Repeated training of certain fingers
can lead to an increase in cortical space mapped to those digits.
Key Takeaways
• Receptive fields become more complex as information moves through the touch
pathway
• Lateral inhibition enhances edges and borders by affecting the perceived stimulus
strength
• Mechanoreceptor afferents synapse in the dorsal column nuclei in the medulla.
Information then decussates and synapses in the ventral posterior nucleus of the
thalamus before traveling to the primary somatosensory cortex
◦ Sensory axons from the lower body synapses in the gracile nucleus in the
dorsal column
◦ Sensory axons from the upper body synapses in the cuneate nucleus in the
dorsal column
◦ Information from the neck and body synapse in the ventral posterior lateral
nucleus of the thalamus
◦ Information from the head and face synapse in the ventral posterior medial
nucleus of the thalamus
• The primary somatosensory cortex is organized in a somatotopic map

• The cortex is plastic and connections can change with experience
Test Yourself!
Additional Review
1. What is the anatomical name of the primary somatosensory cortex?

2. After somatosensory information leaves the brainstem, it must relay through which
structure before reaching the primary somatosensory cortex?
Answers
Attributions
Media Attributions
• TouchReceptiveFieldCenter © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• Touch Receptive Field Surround © Casey Henley is licensed under a CC BY-NC-SA
• Touch Lateral Inhibition © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Touch Pathway From Body © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Touch Pathway From Face © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Touch Pathways © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Postcentral Gyrus © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Somatotopic Map © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Sensory Homonculus © Mpj29 adapted by Valerie Hedges is licensed under a CC BY-SA
• Somatosensory Streams © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• CorticalPlasticity © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Digit merge © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
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538 | PAIN AND NOCICEPTION
39.
PAIN AND NOCICEPTION
Pain is a complex experience that has somatosensory,

psychological, and affective components. Nociception is
a sensory process that provides signals that trigger pain
Resources when in the presence of a noxious stimulus. Detection and
avoidance of pain is a highly-adaptive behavior that can
improve the odds of survival chances of an animal. When
we feel pain, we are more likely to protect the injured site
• Glossary terms to prevent further damage, making the sensation of pain
• Key Takeaways beneficial to our overall well-being.
more
Nociceptors
Pain detection is carried out by nociceptors that are
located in the periphery at the surface of the skin. Nociceptors can detect a variety of noxious stimuli,
ranging from crush (mechanical), acid (chemical), and high heat (thermal). They are expressed on free
nerve endings that innervate the skin. Many pain-detecting neurons respond to more than one type of
noxious stimulus and are called polymodal nociceptors.
These free nerve endings are found closest to the surface of the skin when compared to other
mechanoreceptors. Similar to other somatosensory mechanoreceptors, the sensory end of the
pseudounipolar neurons have their cell soma in the dorsal root ganglion. When a nociceptor is
activated, it causes the opening of mechanically-gated ion channels that allow for cation influx,
resulting in cell depolarization.
Once depolarization occurs in response to a painful stimulus, action potential propagation depends
on the action of voltage-gated sodium and potassium channels. Lidocaine, a common local anesthetic,
dulls pain by blocking these voltage-gated sodium channels, preventing signal transmission. A special
PAIN AND NOCICEPTION | 539
type of sodium channel, Na1.7, is present only in nociceptor fibers. A mutation in this ion channel
that prevents it from functioning can results in the inability to feel pain.
Activation of nociceptors usually occurs in response to tissue damage or the threat of damage.
Depending on the type of stimulus, either Aδ fibers or C fibers are activated to transmit information
to the CNS. Nociceptors are located throughout the body in skin, muscles, and viscera, but there are
no nociceptors in the CNS.
The Aδ fibers have small receptive fields which allows for precise location of pain. They are lightly
myelinated, smaller in diameter (~5 μM), and transmit signals slower than the Aβ fibers that transmit
touch information, around ~25 m/s. There are 2 types of Aδ fibers. Type Aδ I fibers have a low
threshold for mechanical stimulation and activate following intense pressure or an incision on the
skin. Type Aδ II fibers have a low threshold for thermal stimulation and activate in extremely hot or
extremely cold environments.
Type C fibers tend to be polymodal and are activated by a range of stimuli including mechanical,
chemical, and thermal. They have larger receptive fields, which results in a lower ability to locate pain.
The unmyelinated C fibers have an even smaller diameter (~1 μm) and slower conduction velocity (~1
m/s) than the Aδ fibers. The C fibers carry upwards of 70% of noxious signals within the body.
Figure 39.1. Free

nerve endings in
the skin and
other tissues
respond to tissue
damage.
Activation of
these nociceptors
lead to perceived
pain sensation.
Nociceptor fibers
can either by
unmyelinated (C
fibers) or lightly
myelinated (A
delta fibers).
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The Aδ and C fibers transmit information to the CNS at different speeds because of their different
myelination levels. We can often perceive this difference in the form of “first pain” and “second pain”.
Think about a time where you were injured, perhaps you hit your thumb with a hammer. Immediately,
you might have felt an intense, sharp, and localized pain – first pain, but then a milder, burning or
aching pain, that was more widespread that continue longer – second pain. The myelinated Aδ fibers
are responsible for first pain transmission whereas the unmyelinated C fibers are responsible for second
pain transmission.
Figure 39.2. A
delta pain fibers
have thin
myelination,
whereas C fibers
have none. This
allows A delta
fibers to transmit
information to
the CNS faster,
resulting in the
perception of the
sharp, first pain
after an injury. C
fibers transmit
information
slower, causing
the dull, aching,
second pain
perception. ‘Pain
fibers’ by Casey
Henley is
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Pathway to the Brain
Spinal Cord Branching

Primary afferent pain fibers have their cell bodies located in the dorsal root ganglion, like the fibers
of the mechanoreceptors responsible for touch. The axons of these first-order neurons enter the
ipsilateral dorsal side of the spinal cord, and then they branch and travel up and down the spinal cord
a couple of segments in a white matter region of the spinal cord just posterior to the dorsal horn called
Lissauer’s tract. All the branches terminate in the dorsal horn.
Figure 39.3.
Nociceptors cell
bodies are
located in the
dorsal root
ganglia. Axons
enter the spinal
cord and then
branch and
ascend and
descend a short
distance. These
branches travel
in a region called
Lissauer’s tract.
The neurons
make synaptic
connections in
the dorsal horn.
’Lissauer’s tract;
by Casey Henley
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The primary pain afferents coming from the periphery release glutamate as their neurotransmitter.
These afferents can also release the neuropeptide Substance P following periods of high stimulation.
Spinothalamic Pathway
The nociceptor fibers (first-order sensory neurons) make synaptic contact with second-order neurons
in the dorsal horn of the spinal cord. These neurons immediately cross the midline, or decussate, and
then ascend to the brain through the anteriolateral aspect of the spinal cord and brainstem via the
spinothalamic tract. The axons terminate in the ventral posterior lateral nucleus of the thalamus.
The thalamic neurons (third-order neurons) then project to the primary somatosensory cortex
located in the postcentral gyrus in the parietal lobe.
Like the touch pathway (Dorsal column medial lemniscus pathway), the pain pathway
(spinothalamic pathway) is made up of three neurons that ultimately transmit the sensory information
to the appropriate area of the cortex. The first order neuron is the sensory nociceptor neuron, the
second order neuron is located within the spinal cord, and the third order neuron is located in the
thalamus, that extends out to the primary somatosensory cortex.
Figure 39.4. Pain

and temperature
information from
the neck and
body travels
through the
spinothalamic
pathway, named
for structures
within the
pathway. Axons
enter the spinal
cord and
terminate in the
dorsal horn.
Second-order
neurons
decussate and
ascend to the
brain.
Information
continues to the
thalamus, and
then reaches the
somatosensory
cortex. Details of
the pathway are
found in the text.
‘Pain Pathway
from Body’ by
Casey Henley is
licensed under a
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Commons
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Trigeminothalamic Pathway
Nociceptors in the face and head send info to the brain primarily through cranial nerve V, the
trigeminal nerve. The first-order neurons have their cell bodies in the trigeminal ganglion, located
just outside of the brainstem. The fibers enter the brainstem and descend to the spinal trigeminal
nucleus in the medulla, where they synapse on a second-order neuron. The second-order neurons
cross the midline and project up to the ventral posterior medial nucleus of the thalamus. These
neurons then send projections to the face region of the somatosensory cortex.
Figure 39.5. Pain

and temperature
information from
the head and
face travels
through the
trigeminothalami
c pathway. Axons
enter the
brainstem at the
level of the pons
and descend to
the medulla.
They then
decussate before
traveling to the
thalamus and
somatosensory
cortex. Details of
the pathway are
found in the text.
‘Pain Pathway
from Face’ by
Casey Henley is
licensed under a
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Figure 39.6. To
compare the two
pathways, pain
information
comes in from
the periphery.
For the body, the
peripheral
nociceptor fiber
travels via a
spinal nerve to
the cell body
located in the
dorsal root
ganglion, which
sits just outside
the spinal cord.
The axon then
enters the spinal
cord and
synapses on a
second-order
neuron in the
dorsal horn of
the spinal cord.
The
second-order
neuron crosses
the midline and
then projects to
the ventral
posterior lateral
nucleus of the
thalamus via the
spinothalamic
tract. The
thalamic
third-order
neuron projects
to the primary
somatosensory
cortex in the
parietal lobe. For
pain information
from the face,
the peripheral
nociceptor fibers
travels to the
trigeminal
ganglion via
cranial nerve V.
The ganglion sits
outside of the
brainstem, and
the axons then
enter the
brainstem,
descend and
synapse on the
spinal trigeminal
nucleus. The
second-order
neuron travels to
the ventral
posterior medial
nucleus of the
thalamus, and
the third-order
neuron projects
to the primary
somatosensory
cortex in the
parietal lobe.
‘Pain Pathways’
by Casey Henley
is licensed under
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In summary, the touch pathway (Dorsal column medial lemniscus pathway) and the pain pathway
(spinothalamic pathway) are made up of three neurons that ultimately transmit the sensory
information between the periphery and the appropriate area of the cortex.
Figure 39.7.
Comparison of
touch and pain
pathways. In the
touch system
(dorsal column
system) the
first-order
neuron enters
the spinal cord,
and staying
ipsilateral,
ascends to the
medulla where it
synapses on the
second-order
medulla neuron.
The medulla
neuron then
decussates the
information to
the contralateral
side of the brain
where it then
ascends via the
medial lemniscus
to the thalamus.
The third-order
thalamic neuron
then
communicates
the information
to the
appropriate area
of the primary
somatosensory
cortex. In the
pain pathway
(spinothalamic
tract) the
first-order
neuron enters
the spinal cord,
and immediately
synapses on the
second-order
neuron within
the spinal cord.
The second order
neuron
decussates the
information to
the contralateral
side of the spinal
cord before it
ascends to the
thalamus via the
spinothalamic
tract. The
third-order
neuron in the
thalamus then
goes to the
appropriate area
of the
somatosensory
cortex.
Dissociated Sensory Loss

Because the sensations of touch and pain decussate at different levels of the CNS as they ascend to the
primary somatosensory cortex for processing, damage to only one half of the spinal cord will result in
a condition referred to as dissociated sensory loss.
Ipsilateral to and below the damaged half of the spinal cord, individuals will have decreased touch
sensation but normal pain sensation. Contralateral to and below the damaged half of the spinal cord,
there will be normal touch sensation, but decreased pain sensation. Sensation above the damage will be
unchanged.
Figure 39.8.
Dissociated
sensory loss.
When the right
half of the spinal
cord is damaged,
the area
surrounding the
damage will
experience
complete loss of
sensation.
Ipsilateral and
below the lesion
(colored blue)
will experience
decreased touch
sensation due to
the first-order
sensory touch
neuron staying
ipsilateral as it
ascends to the
medulla. Pain
information
ipsilateral and
below the lesion,
however,
immediately
decussates to the
contralateral side
of the body
before ascending
to the medulla,
and therefore
will not be
affected by the
lesion.
Contralateral and
below the lesion
(colored yellow)
will experience
decreased pain
sensation due to
the first-order
pain neurons
immediately
decussating to
the right side of
the body, where
the lesion will
stop the pain
information from
ascending to the
medulla. Touch
sensation
contralateral and
below the lesion
will be
unaffected as it
will ascend to the
medulla on the
left side of the
body.
‘Dissociated
sensory loss’ by
Valerie Hedges is
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License.
Referred Pain
One of the warning signs that a person is having a heart attack is perception of pain in the shoulders or
medial aspect of the left arm. This sensation is called referred pain: the feeling of pain at a site separate
from where an injury is located. Referred pain happens when the nervous system is unclear about how
to process signals from an internal organ, and the brain interprets those afferent signals as bodily pain.
Injury at different internal organs cause different patterns of somatic pain: liver injury may present as
pain in the shoulder blades, lung cancer sometimes causes shoulder pain, and kidney stones can cause
pain in the lower back, abdomen, and sides. Even the headache-like pain you get from a “brain freeze”
from drinking cold liquids too quickly is a form of referred pain.
It is unclear what causes referred pain. The convergence projection theory suggests that the afferent
projections from the internal organs and the nociceptive somatosensory neurons of the skin form
synapses onto the same population of spinothalamic tract secondary neurons. When these secondary
neurons project towards the brain, there is no ability to differentiate the origin of the signal coming
from the primary neuron.
The central sensitization theory suggests that the neurons of the spinal cord change in their
excitability threshold with prolonged exposure to injurious stimuli. So, when an injured internal organ
repeatedly sends signals into the secondary neurons, a host of neurotransmitters cause sensitization of
the nociceptive signaling, which are now activated under non-noxious conditions.
Figure 39.9.
Referred pain.
The nervous
system will
interpret signals
from internal
organs as pain in
certain areas of
the body. This
figure shows the
different areas of
the body that
can experience
referred pain that
originates from
an organ.
Pain Regulation
Pain Disorders
In healthy individuals, nociceptors that detect pain send those signals into the spinal cord. But in
people experiencing allodynia, noninjurious tactile stimuli, such as strands of hair brushing across
your forehead or the feeling of a shirt resting on your shoulders, cause the sensation of pain. Diabetes,
physical trauma, or postherpetic neuralgia may cause allodynia. Drugs that modify the action potential
firing properties of the afferent somatosensory pathway, such as topical anesthetics (voltage-gated
sodium channel inhibitors) or calcium channel antagonists, can help relieve the pain. A related disorder
is hyperalgesia, an abnormally heightened perception and increased persistence of pain. When you
experience a cut on your finger, and the area surrounding the cut is reddened and painful, that is the
area of hyperalgesia. Both allodynia and hyperalgesia can be the result of sensitization, where repeated
firing of the neurons increase action potential firing of pain neurons. Sensitization within this system
can lead to functional and structural changes wherein weak stimuli that previously did not cause pain
will now result in pain perception. Sensitization can occur in first-order, second-order, or third-order
neurons within the pain pathway.
Figure 39.10. Pain

disorders.
Allodynia is a
pain disorder
that causes pain
to be perceived
under conditions
that normally are
not painful.
Hyperalgesia
causes an
abnormally
heightened
perception of
pain compared to
normal pain
perception.
Allodynia and hyperalgesia can be symptoms of neuropathic pain, a broad category of pain conditions
resulting from damage to the nervous system. People experiencing pain even in the absence of pain-
producing stimuli may be diagnosed with chronic pain. Chronic pain may be localized or widespread,
affecting large areas of the body at once. Up to 10% of the global population may experience chronic
pain, and a variety of risk factors contribute, from advanced age, being male, low socioeconomic
status, unhealthy lifestyles, and a history of surgical intervention. Chronic pain can lead to significantly
shorter lifespans and may contribute to the opioid misuse epidemic.
A philosophically-opposite condition is called congenital insensitivity to pain (CIP). People with
CIP are incapable of perceiving pain, no matter how severe. CIP is a rare genetic condition that is
observed from birth. People with CIP have significantly shorter lifespans since they do not sense that
their bodies may be injured and may not learn to avoid exposure to damaging stimuli. Some patients
may walk for days on a compound fracture of the shin bone, or never even notice that their back is
broken. CIP is associated with mutations in the SCN9A gene, which codes for a component of the
voltage-gated sodium channels that are expressed in nociceptors.
Descending Pain Regulation- Gate Control Theory

Individuals can have horrible injuries, yet not experience any pain in the moment. How can this occur?
One theory for how this is accomplished is ‘The Gate Control Theory of pain modulation’ which
postulates that there are descending pathways that start in the brain and signal to the spinal cord that
acts to inhibit, or “gate”, the pain signals from ascending to the brain and being perceived as pain. One
brain area implicated in this pathway is the periaqueductal gray (PAG) matter within the midbrain.
Neurons with their cell bodies within the PAG have axons that descend to the Raphe nuclei within
the medulla. The raphe nuclei neurons then descend the spinal cord and release serotonin onto the
second-order spinal pain neuron. The second-order pain neuron is therefore receiving synapses from
both the first-order pain neuron that is transmitting the pain signal from the periphery and the raphe
nuclei neuron carrying the descending signal from the CNS. The serotonin released by the raphe nuclei
neuron acts to inhibit activity of the second-order pain neuron, thereby inhibiting the pain signal from
ascending to the brain. In what situations do the PAG neurons ‘gate’ the pain signals from ascending
to the brain and being perceived as pain? That answer is less clear, though we do understand that the
gating of the pain signal eventually stops and the pain is able to be perceived.
Figure 39.11. Gate

Control Theory of
Pain regulation.
Neurons within
the
periaqueductal
gray matter of
the midbrain
descend to the
raphe nuclei
within the
medulla. The
raphe nuclei
neurons descend
down the spinal
cord and release
serotonin onto
the second-order
pain neurons
within the spinal
cord. The
serotonin acts to
inhibit the pain
signal from being
transmitted to
the second order
neuron, and thus
inhibits the pain
signal from
ascending to the
brain and being
perceived as
pain. ‘Gate
Control Theory’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
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License.
Endogenous Opioids
The experience of pain can be altered through drugs that interact with the brain. An analgesic
decreases pain without a loss of consciousness, whereas central anesthetics cause a loss of consciousness
and completely eliminate all incoming sensory experiences, including pain and somatosensory
information.
Opioids are drugs that are very effective at short-term pain relief. They are derived from the poppy
plant and include opium, morphine, codeine, heroin, and analogues of those substances. Opioids
bind strongly to several types of opioid receptors (all G-protein-coupled receptors) that are located
throughout the brain, with especially high receptor density in areas such as the PAG, raphe nuclei, and
in the spinal cord. Antagonists for opioid receptors block the effects of opioids and include drugs like
naloxone and naltrexone.
The brain also makes its own endogenous opioids including endorphins, enkephalins, and
dynorphins, that bind to these same receptors within the brain. Endogenous opioids are involved in
various functions within the brain including nociception, respiration, cardiovascular, neuroendocrine,
food intake, learning and memory, and many others. When opioids (either exogenous or endogenous)
bind to their receptors, they modulate incoming pain information and provide temporary pain relief.
Opioids are known to have effects on mood and can produce euphoric feelings. Unfortunately, they
also have several side effects, most notably drug tolerance and dependence.
Phantom Limb Phenomenon

Individuals that have undergone amputation of a limb can experience ‘phantom limbs’, which give
the sensory experience that the amputated limb is still present. In these cases, the sensory neurons can
no longer provide sensory or pain information from the amputated limb, yet somatosensory and pain
perception persists. Some individuals that experience phantom limbs can also experience phantom
limb pain, where pain is experienced in a limb that has been removed.
Many physicians believed that phantom limbs were caused by irritation of nerve endings that
remained in the limb stump after amputation. However, the research by the neuroscientist Dr. V.S.
Ramachandran demonstrated that cortical plasticity played a major role in producing phantom limbs.
Specifically, Dr. Ramachandran believed that cortical plasticity within the somatosensory cortex was
responsible for the sensation of phantom limbs. He hypothesized that adjacent somatosensory maps
within the cortex blurred together following amputation, causing the brain to rewire in the absence of
incoming sensory information.
The following case study demonstrated this somatosensory reorganization following amputation.
Dr. Ramachandran worked with an individual named Tom Sorenson that had lost an arm in a car
accident. Tom was experiencing an itch on his phantom arm that he could not scratch. Due to the fact
that the cortical maps for the face and the arm are adjacent to each other within the somatosensory
cortex, Ramachandran methodically used a swab to stroke different areas of Tom’s face. Tom reported
that in certain areas of his face, he could also feel the swab on his phantom limb. Functional brain scans
confirmed that the maps for the face and the arm had blurred together.
Ramachandran believes that phantom limb pain stems from a “learned paralysis” that occurs when
an individual sends out a motor command to move a phantom limb but receives no proprioceptive
or visual confirmation that the phantom limb has moved. After this experience is repeated, the brain
wires in such a way that it learns that the phantom limb does not move, leading to feelings of paralysis
and pain associated with the phantom limb. To treat this “learned paralysis” in the phantom,
Ramachandran developed mirror box therapy. In mirror box therapy, an amputee uses a mirror to
produce a reflection of the intact limb opposite the amputated limb. By looking at this reflection, the
individual experiences visual feedback that the amputated limb is intact and can now move. Looking
at the movement of the “phantom” limb in the mirror, provides powerful input to the nervous system
and acts to alleviate the feelings of paralysis and pain associated with the phantom limb, and effectively
“unlearn” the paralysis and feelings of pain.
Figure 39.12.
Mirror Box
Phantom Limb
therapy. The
mirror box was
developed by Dr.
V.S.
Ramachandran
and is used to
treat individuals
that experience
phantom limb
pain. The
reflection of the
intact limb in the
mirror provides
powerful visual
feedback that
the amputated
limb is intact and
can move in a
non-painful way.
This sensory
experience
produces
plasticity within
the brain to
unlearn the
previous
paralysis and
pain associated
with the
phantom limb.
Key Takeaways
• Nociceptors are free nerve endings located throughout the body.

• Pain information is carried on Aδ and C afferent fibers that are separately responsible
for first and second pain, respectfully.
• The spinothalamic pathway is a three neuron path that carries pain information from
the body to the somatosensory cortex and the trigeminothalamic pathway carries
pain information from the head and face to the somatosensory cortex.
• Dissociated sensory loss results from damage to one half of the spinal cord.
• Referred pain is the feeling of pain at a site separate from where an injury is located.
• Allodynia and hyperalgesia are disorders of pain perception and can occur following
sensitization of neurons within the pain pathway.
• Descending pathways from the brain can modulate pain perception.
• The opioid system within the body is important in modulating pain information.
• Phantom limb pain can occur in individuals with an amputated limb. Mirror box
therapy can treat those with phantom limb pain.
Attributions
Media Attributions
• Nociceptors © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial

ShareAlike) license
• Pain fibers © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Lissauer’s tract © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Pain Pathway from Body © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Pain Pathway from Face © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Pain Pathways © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Touch and Pain pathways © OpenStax College adapted by Valerie Hedges is licensed under a CC
BY (Attribution) license
• Dissociated sensory loss © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Referred Pain Chart © Open Stax College is licensed under a CC BY (Attribution) license
• Pain Intensity © Richard Lennertz adapted by Valerie Hedges is licensed under a CC BY
• Descending pain regulation © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Phantom limb mirror box © Golan Levin adapted by Valerie Hedges is licensed under a CC BY
MOTOR SYSTEMS | 561
PART VI
MOTOR SYSTEMS
562 | MOTOR SYSTEMS
SPINAL MOTOR CONTROL AND PROPRIOCEPTION | 563
40.
SPINAL MOTOR CONTROL AND

PROPRIOCEPTION
The motor system refers to the nerve cells that are used to
control our body. The key roles of the motor system are to
plan, control, and execute voluntary (deliberate) Resources
movements, and to control involuntary (subconscious or
automatic) functions, such as digesting food. The motor
system is sometimes described as a top-down process: in a
• Glossary terms
voluntary movement, neural activity in the frontal lobe
• Key Takeaways
sends commands down to motor neurons located in the
• Test Yourself
brainstem or spinal cord, which in turn activate muscle
groups. In reality, motor control is more of a loop, rapidly
communicating between the sensory cortex and motor
cortex. Sensory information about limb position, posture,
and objects in contact with the skin inform the descending motor plan. Simultaneously, the motor
plan provides predictions about upcoming movement.
There are multiple levels of control. Within the spinal cord, simple reflexes can function without
higher input from the brain. Slightly more complex spinal control occurs when central pattern
generators function during repetitive movements like walking. The motor and premotor cortices in
the brain are responsible for the planning and execution of voluntary movements. And finally, the
basal ganglia and cerebellum modulate the responses of the neurons in the motor cortex to help with
coordination, motor learning, and balance.
This lesson explores the lowest level of control at the level of the spinal cord.
564 | SPINAL MOTOR CONTROL AND PROPRIOCEPTION
Figure 40.1.
Motor output is
controlled at
multiple levels: A.
Spinal cord and
spinal neurons, B.
Motor (dark blue)
and premotor
(light blue)
cortices, C. Basal
ganglia (a
subcortical
structure shown
in light blue) and
cerebellum
(yellow). ‘Motor
Control Levels’ by
Casey Henley is
licensed under a
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Commons
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Non-Commercial
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BY-NC-SA) 4.0
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License.
Alpha Motor Neurons

Muscle fibers are innervated by alpha motor neurons. Alpha motor neurons are also called lower
motor neurons because they are not located in higher brain areas. These cells are the only cells that
directly command muscle contraction. The cell bodies of the alpha motor neurons are located in the
central nervous system in the ventral horn of the spinal cord. Their axons leave the spinal cord via the
ventral roots and travel to the muscle via efferent peripheral spinal nerves.
Figure 40.2.
Alpha motor
neurons are
located in the
ventral horn of
spinal cord. Their
axons, which are
efferent fibers,
travel to the
muscles via
spinal nerves.
‘Alpha Motor
Neurons’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Like the sensory systems, the motor system is also organized in a topographic fashion, referred to as
‘somatotopic organization’. Within the spinal cord, alpha motor neurons that innervate muscles in the
arms and legs are located in the lateral portion of the ventral horn, whereas alpha motor neurons that
innervate muscles in the trunk are located in the medial portion.
Figure 40.3. The

ventral horn is
organized in a
topographic
manner, with
proximal muscles
(like those in the
trunk) located
more medially
than distal
muscles (like the
arms or legs).
Additionally,
motor neurons
are organized by
function with
extensor motor
neurons located
together and
flexor neurons
located together.
‘Spinal Cord Map’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Location of Alpha Motor Neurons

The structure of the spinal cord is reviewed in Chapter 26. Examining cross sections of the spinal
cord at different levels reveals that there is a non-uniform distribution of lower motor neurons. This
is determined by the size of the ventral horn at the different levels of the spinal cord. At the cervical
enlargement the larger ventral horns are due to the presence of more lower motor neurons that
function in movement of the arms. At the lumbar enlargement the larger ventral horns are due to the
presence of more lower motor neurons that function in movement of the legs.
Figure 40.4.
Ventral horn
anatomy at
different levels of
the spinal cord.
There are 4
divisions of the
spinal cord:
cervical, thoracic,
lumbar, and
sacral. The size of
the ventral horn
(colored in red),
where α motor
neuron cell
bodies are
located, changes
at the different
levels of the
spinal cord. The
ventral horn is
enlarged in the
cervical and
sacral regions of
the spinal cord
due to the
increased
number of motor
neurons that are
needed for motor
control of the
arms and legs.
The ventral horn
is smaller in the
thoracic region
due to decreased
muscle
innervation in
this area.
Neuromuscular Junction
The lower motor neurons communicate with muscle fibers (muscle cells) at the neuromuscular
junction (NMJ). The NMJ is similar to other chemical synapses, however the postsynaptic cell is a
muscle cell separated by about 30 nm. The presynaptic cell is the motor neuron and the postsynaptic
site is the sarcolemma, the cell membrane of the long cylindrical muscle fibers (muscle cells). The
neuromuscular junction is one of the largest synapses in the body and one of the most well-studied
because of its peripheral location.
The neuromuscular junction is located midway down the length of the muscle fiber. The muscle
fiber will contract in response to depolarization traveling down the sarcolemma of the muscle fiber.
The synapse is located midway down the length of the muscle fiber so that the postsynaptic signal can
travel in both directions down the long muscle fiber and quickly activate a contraction along the entire
cell.
Acetylcholine is the neurotransmitter released at the neuromuscular junction (NMJ), and it acts
upon ligand-gated, non-selective cation channels called nicotinic acetylcholine receptors that are
present in postjunctional folds of the muscle fiber. Nicotinic acetylcholine receptors allow the influx of
sodium ions into the muscle cell. The depolarization will cause nearby voltage-gated channels to open
and fire an action potential in the muscle fiber. In a healthy system, an action potential in the motor
neurons always causes an action potential in the muscle cell. The action potential leads to contraction
of the muscle fiber.
Figure 40.5. The

ionotropic
nicotinic
acetylcholine
receptors in the
postjunctional
folds of the
muscle fiber are
non-selective
cation channels
that allow the
influx of sodium
and the efflux of
potassium The
depolarization of
the cell by the
sodium influx will
activate nearby
voltage-gated
ion channels.
‘NMJ Ion Flow’ by
Casey Henley is
licensed under a
Creative
Commons
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To review, an action potential traveling down the motor neuron (presynaptic cell) will cause the release
of acetylcholine into the synapse. Acetylcholine binds to postsynaptic nicotinic acetylcholine receptors
that are located in the folded sarcolemma (increasing surface area), causing depolarization of muscle
fibers and ultimately muscle contraction.
Acetylcholinesterase, an enzyme that breaks down acetylcholine and terminates its action, is
present in the synaptic cleft of the neuromuscular junction. Muscle contraction must be tightly
controlled. Thus, the actions of acetylcholinesterase are very important to cease muscle contraction
quickly.
Figure 40.6. The

neuromuscular
junction (NMJ) is
the synapse
between a motor
neuron and a
muscle fiber.
Acetylcholine is
released at the
NMJ and acts on
nicotinic
acetylcholine
receptors located
in the
postjunctional
folds of the
muscle fiber.
Neurotransmitter
action is
terminated by
breakdown by
acetylcholinester
ase.
‘Neuromuscular
Junction’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Clinical Application: Myasthenia gravis
Myasthenia gravis (MG) is an autoimmune disorder characterized by muscle weakness,

resulting in difficulty with speech, trouble with movement and swallowing, drooping
eyelids, and double vision. Each year, an estimated 20 out of a million people get diagnosed
with MG.
The muscle weakness seen in MG results from immune system-mediated destruction of the
nicotinic acetylcholine receptors expressed at the NMJ. Thus, when the lower motor neuron
releases acetylcholine, the muscle cells are unable to detect this release, so they fail to
contract appropriately.
One therapeutic strategy involves inhibition of acetylcholinesterase, the enzyme that

degrades acetylcholine. This causes the synaptic acetylcholine to remain in the synapse
longer, increasing the chance that receptors get activated. Alternatively, autoimmune
diseases like MG can be improved with immunosuppressant therapy. With successful
treatment, MG usually does not result in changes in lifespan.
40.7. Myasthenia
Gravis. Individuals
with Myasthenia
Gravis can have
symptoms such
as drooping eye
lids and weak
facial muscles.
Motor Units
Importantly, there are many more muscles cells than there are motor neurons. One alpha motor
neuron can innervate multiple fibers within one muscle due to the branching of motor neuron axons.
Each axon terminal synapses (innervates) a single muscle fiber. A motor neuron and all the fibers
innervated by it are called a motor unit. The muscle fibers within one motor unit are often spread
throughout the muscle to spread the contraction throughout the full muscle. Further, motor units in a
muscle usually contract asynchronously to help protect the muscle from fatigue. A graded contraction
of the muscle is produced by activating varying numbers of motor units.
Motor units differ in size. Small motor units are motor units that innervate fewer muscle fibers and
thus control fine movements. Small motor units are located in the eyes and fingers, both of which
function in fine and precise movements. Large motor units innervate many muscle fibers and are
typically found in weight-bearing muscles like the thighs.
The group of motor neurons that innervate all the fibers of one muscle is called a motor pool.
Figure 40.8
Motor neurons
can innervate
more than one
muscle fiber
within a muscle.
The motor
neuron and the
fibers it
innervates are a
motor unit. Three
motor units are
shown in the
image: one blue,
one green, one
orange. Those
three motor units
innervate all the
muscles fibers in
the muscle and
are the motor
pool for that
muscle. ‘Motor
Unit and Pool‘by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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License.
Types of Motor Units

In addition to the size of the motor unit, the types of muscle fibers that are innervated by motor units
can also differ. There are three different types of motor units:
1. Slow motor units. Slow motor units are slow to contract and generate less force but can work
for a long time. They are used in endurance exercise like jogging.
2. Fast fatigue-resistant motor units. Fast fatigue-resistant motor units are quick to contract
(though not as fast the fast fatigable motor units). These motor units generate more force that
then slow motor units, but are much more resistant to fatigue than the fast fatigable motor
units.
3. Fast fatigable motor units. Fast fatigable motor units are quickest to contract and generate the
most force. These motor units are more prone to fatigue due to decreased number of
mitochondria within the muscles. Fast fatigable motor units generate a lot of force quickly, but
also tire quickly. They are used mostly in high intensity exercise like lifting weights and
sprinting.
Figure 40.9.
Force generated
by different
types of motor
units. Slow motor
units are slow to
contract and
exert very little
force (shown in
pink).
Fast-fatigue
resistant motor
units are quicker
to contract and
generate more
force than slow
motor units.
Fast-fatigable
motor units are
fastest to
contract and
generate the
most force.
‘Motor unit force’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Muscle Activation
Action Potential Triggered in a Muscle

When an action potential is triggered in a muscle it causes a muscle twitch (contraction) that is
followed by a period of relaxation. A muscle twitch shows an increase in tension after a short delay
(latent period). During the contraction period, the muscle tension increased, and then has a long
relaxation period, causing the muscle tension to be increased long after the initial stimulus.
Figure 40.10.
Muscle twitch. A
stimulus will
cause a muscle to
experience a
twitch. A twitch
is characterized
by a short latent
period following
the stimulation,
followed by
contraction,
which increases
muscle tension.
Following
contraction, the
muscle has a
longer relaxation
period until
tension returns
to baseline.
When a stimulus occurs during the long relaxation period following a muscle twitch, the newly
generated muscle twitch will summate to increase the strength of the overall muscle contraction.
Shortening the time between stimuli will result in unfused tetanus and if stimuli are very close
together will result in fused tetanus. Therefore, a higher rate of action potentials in the alpha motor
neuron will generate more muscle contraction.
Figure 40.11.
Unfused and
fused tetanus in
muscles. Due to
the long
relaxation period
in muscles, if
another stimulus
is delivered
during the
relaxation period,
the muscle
twitches
summate to
produce an
increase in
muscle tension,
referred to as
‘unfused
tetanus’. When
stimuli are
delivered very
close together in
time, such that
the muscle does
not have time to
relax, then
maximal muscle
tension results,
also called ‘fused
tetanus’.
Muscle Recruitment
In addition to the rate of action potentials changing the force of the muscle contraction, muscle
recruitment can also increase the strength of contraction within a muscle. When generating motor
activity, the smallest motor units will be activated to contract first due to their size and increased
excitability to move the load. Increasingly larger motor units are recruited to lift heavier loads.
Recruitment of motor units allows for us to generate appropriate muscle tension to move a given load.
For example, if you need to pick up a pencil, then we do not need to use the same force as if we were
trying to pick up a 20-pound weight. Only smaller motor units would be activated to pick up the
pencil, and increasingly larger motor units would be recruited to lift the heavier load of the 20-pound
weight.
Figure 40.12.
Muscle
Recruitment.
Motor units are
recruited in size
order to move
increasingly
heavier loads. For
a light load that
requires minimal
muscle tension,
only Motor Unit 1
(shown in red) is
recruited. For a
medium-heavy
load, Motor Unit
2 (shown in blue)
is recruited, in
addition to Motor
Unit 1. The
heaviest loads
require the
additional
recruitment of
Motor Unit 3
(shown in
orange).
Alpha Motor Neuron Inputs

The alpha motor neurons that directly cause muscle contraction receive inputs from three different
sources.
1. Sensory cells from the dorsal root ganglion that provide sensory information from the
muscles through proprioception.
2. Upper motor neurons from the motor cortex in the brain and brain stem that are
responsible for initiating voluntary movement.
3. Interneurons in the spinal cord. These represent the largest input to the alpha motor neurons
and can either provide excitation or inhibition to the alpha motor neuron.
Figure 40.13.
Alpha motor
neuron inputs.
Alpha motor
neurons located
in the ventral
horn of the spinal
cord receive
inputs from the
sensory neurons
collecting
information from
the periphery,
upper motor
neurons from the
brain and
brainstem, and
from a
population of
interneurons
within the spinal
cord. ‘Alpha
motor neuron
inputs’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Proprioception
Raise your arms above your head. Even without seeing your arms, your nervous system has mechanisms
that inform you about the location and position of your body parts, including how much your joints
are bent. This sense is called proprioception and is critically important for coordinated movement
and motor reflexes that contribute to those tiny, rapid adjustments that are made while maintaining
balance. Proprioceptive information ascends through the spinal cord and into the brain via the dorsal
column-medial lemniscus tract. Proprioception is also processed in the primary somatosensory
cortex.
Proprioception refers to the “body sense” that informs us about how our bodies are positioned and
moving in space. Proprioceptors are receptors that provide proprioception information.
There are two main types of proprioceptors:
1. Muscle spindles measure muscle stretch (muscle length) and transmit this sensory information
via 1a sensory afferent fibers. Muscle spindles are nested within and arranged parallel to the
extrafusal muscle fibers.
2. Golgi Tendon Organs measure muscle tension and transmit this sensory information via 1b
sensory afferent fibers. Golgi tendon organs are located between the extrafusal muscle fibers and
their points of attachment at the bone.
Muscle Spindles
Extrafusal and Intrafusal Muscle Fibers
Muscle spindles are fibrous capsules that are located within muscles. Intrafusal muscle fibers are
special muscle fibers that are located within the fibrous capsule of the muscle spindle. The intrafusal
muscle fibers are innervated by gamma motor neurons that will cause them to contract.
Extrafusal muscle fibers, however, make up the bulk of the muscle and are located outside of the
muscle spindle. The extrafusal muscle fibers are stimulated to contract by the alpha motor neurons.
Figure 40.14.
Type I primary
afferent sensory
axons wrap
around the
intrafusal fibers
within the
muscle spindle,
located deep in
the muscle.
When the muscle
stretches, these
sensory neurons
are activated.
Extrafusal muscle
fibers are located
outside of the
muscle spindle
and make up the
bulk of the
muscle. ‘Muscle
Spindle’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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License.
Muscle Spindles Function
Group 1a sensory afferent axons, which have a large diameter and are heavily myelinated, wrap
around the intrafusal fibers contained within the muscle spindle. These sensory afferent fibers will
signal when the intrafusal fibers of the muscle spindle are experiencing stretch and communicate
information about muscle length.
Within the spinal cord, a single sensory 1a afferent axon synapses on every alpha motor neuron
within the motor pool that innervates the muscle that contains the muscle spindle. This allows for a
fast and powerful contraction of the muscle in response to a change in muscle stretch.
Gamma Motor Neuron Function
When the muscle experiences a stretch and the extrafusal fibers are stretched, the muscle spindle
and the intrafusal fibers are also stretched (due to being within the muscle and surrounded by the
extrafusal fibers). When the muscle spindle stretches, the 1a sensory axon will start to fire action
potentials. The sensory axon synapses with an alpha motor neuron that will then cause the extrafusal
muscle fibers to contract. As the extrafusal fibers contract and the muscle shortens, the muscle spindle
goes slack, and the 1a axon will no longer fire action potentials as it is no longer being stretched. The
gamma motor neuron is then activated that innervates the intrafusal muscle fibers, causing the
intrafusal fibers to contract, allowing the muscle spindle to sense stretch again. Therefore, the gamma
motor neuron is critical for allowing the muscle spindle to continue providing information about
muscle stretch even when the muscle has experienced contraction.
Figure 40.15.
Gamma motor
neuron function.
A. A muscle
spindle is shown
within a muscle.
A 1a sensory
afferent is
wrapped around
the muscle
spindle. An
electrode is
measuring an
increase in 1a
sensory afferent
firing rate when
the muscle is
stretched. B. In
response to
stretch, the alpha
motor neuron
that innervates
the extrafusal
muscle fibers has
an increase in
firing rate. At this
time, the muscle
spindle goes
slack and can no
longer
communicate
muscle stretch
information via
the 1a sensory
afferent. C.
Gamma motor
neuron activity
increases to
contract
intrafusal muscle
fibers within the
muscle spindle.
This allows for
the muscle
spindle to
continue to
communicate
muscle length
that is then
sensed by the 1a
sensory afferent.
‘Gamma motor
neuron function’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
International
License.
Golgi Tendon Organ

The Golgi tendon organ is a proprioceptor that measures muscle tension, or the force of contraction.
They also contribute to our detection of weight, as we lift something heavy for example. Golgi tendon
organs are located in the tendon that connects the muscle to bone. The tendon is made up of collagen
fibrils and the Group 1b sensory axons are intertwined within the collagen fibrils. When the muscle
experiences an increase in tension, the collagen fibrils surrounding the 1b sensory axon physically
squeeze the 1b axon, opening mechanically-gated ion channels within the 1b sensory axon.
Figure 40.16.
Golgi tendon
organ. Golgi
tendon organs
are located
within muscle
tendons. A Group
1b sensory axon
intertwines
within the
collagen fibrils of
the muscle
tendon. When
the muscle
experiences an
increase in
tension, the
collagen fibrils
squeeze the 1b
sensory axon,
causing a signal
to travel down
the 1b axon
toward the spinal
cord. ‘Golgi
tendon organ’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
International
License.
The purpose of the Golgi tendon organ is to allow for an optimal range of tension for the muscle and
to protect the muscle from injury due to being overloaded. This is accomplished through a negative
feedback loop controlled by the Golgi tendon organ. When the muscle experiences an increase in
muscle tension, the 1b sensory axon starts to fire action potentials. The sensory neuron synapses onto
an inhibitory interneuron within the spinal cord, which when active will release GABA onto the alpha
motor neuron that innervates the same muscle that experienced the increase in muscle tension to begin
with. When GABA binds to the alpha motor neuron, it will decrease firing of the alpha motor neuron,
leading to a decrease in contraction of the muscle. This is an example of negative feedback as the
increased muscle tension ultimately leads to physiological changes that decrease muscle tension.
Figure 40.17.
Golgi tendon
organ signaling.
An incrase in
tension within
the muscle, will
cause activity of
a 1b sensory
axon that travels
through the
dorsal side of the
spinal cord,
where it
synapses onto an
inhibitory
interneuron
within the spinal
cord. Activation
of the inhibitory
interneuron will
cause it to
release GABA
onto the α motor
neuron within
the ventral horn
of the spinal
cord, inhibiting
the α motor
neuron from
firing. This will
lead to a
decrease in
contraction of
the muscle that
is innervated by
the α motor
neuron. ‘Golgi
tendon organ
signaling’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
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Key Takeaways
• Motor neuron cell bodies are located in the ventral horn of the spinal cord
• Motor neuron axons are located in the peripheral nervous system and travel to
muscles via spinal nerves
• Acetylcholine is released at the neuromuscular junction and acts upon ionotropic
nicotinic acetylcholine receptors
• The spinal cord is topographically organized
• Muscle twitches can summate to increase muscle tension
• A motor unit is an alpha motor neuron and all of the motor fibers that it innervates.
Motor units differ in size, recruitment, and power.
• Muscle spindles and Golgi tendon organs are proprioceptors that communicate
information about the location and position of the body.
Test Yourself!
Additional Review
1. What is the difference between a motor unit and a motor pool?
Attributions
Media Attributions
• MotorControlRegions © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-

• Alpha Motor Neuron © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Spinal Cord Map © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Untitled_Artwork 1 © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
• NMJ Ion Flow © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• NeuromuscularJunction © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Myasthenia Gravis © Posey and Spiller adapted by Valerie Hedges is licensed under a Public
Domain license
• Motor Unit And Pool © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Types of motor units © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Muscle_Twitch_Myogram © OpenStax adapted by Valerie Hedges is licensed under a CC BY
• Twitch_vs_unfused_tetanus_vs_fused_tetanus © Daniel Walsh and Alan Sved adapted by
Valerie Hedges is licensed under a CC BY-SA (Attribution ShareAlike) license
• Motor unit recruitment © Daniel Walsh and Alan Sved is licensed under a CC BY-SA
• Motor neuron inputs © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Muscle Spindle © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Gamma motor neuron function © Valerie Hedges is licensed under a CC BY-NC-SA
• Golgi tendon organ © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• golgi tendon organ signaling © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
SPINAL REFLEXES | 591
41.
SPINAL REFLEXES
Reflexes
Reflexes are involuntary motor responses that are
Resources
performed automatically and independent of brain signals
(although some can be suppressed voluntarily, with extra
effort). Reflexes involve very simple circuits, sometimes
consisting of only two populations of neurons: Sensory
• Glossary terms
information comes in from the periphery, synapsing onto
• Key Takeaways
motor neurons in the brainstem or spinal cord. Reflexes
• Test yourself!
can take place as quickly as 1/100th of a second!
Stretch Reflex
When a muscle is stretched, this stretches the associated muscle spindle, which activates a 1a sensory
afferent neuron that has its cell body located in the dorsal root ganglion. The sensory axon enters the
dorsal side of the spinal cord where it makes two synapses:
1. A direct synapse onto a motor neuron in the spinal cord gray matter. The axon of the motor
neuron exits the spinal cord and innervates the stretched muscle, causing it to contract.
2. A synapse onto an inhibitory interneuron that then synapses onto a separate motor neuron that
innervates the antagonistic muscle.
The myotatic reflex, also called the patellar, or knee-jerk reflex, is an example of a stretch reflex and
occurs in response to activation of the muscle spindle stretch receptors. This reflex is commonly tested
at a doctor’s visit when the doctor taps your knee with a little hammer. This usually results in the lower
592 | SPINAL REFLEXES
leg kicking up slightly. The synaptic communication for this reflex takes place completely within the
spinal cord and requires no input from the brain.
The knee is tapped on the tendon that connects to the quadriceps muscle. The tapping of the
tendon stretches the tendon and as a result causes the quadriceps muscle to stretch, activating the
stretch receptors. Sensory information travels to the dorsal horn of the spinal cord where it synapses on
alpha motor neurons that innervate the quadriceps muscle. Activation of the motor neurons contracts
the quadriceps, extending the lower leg. This is called a monosynaptic communication because there is
only one synapse between the sensory input and the motor output. The reflexive kick is controlled at
the level of the spinal cord and cannot be intentionally suppressed by descending motor pathways no
matter how hard you concentrate.
Figure 41.1. When

the tendon in the
knee is tapped,
the extensor
muscle is
stretched slightly.
This stretch
activates the
Group I sensory
afferent axons
(blue S neuron in
dorsal root
ganglion) from
the muscle
spindles. The
sensory neurons
synapse on and
activate motor
neurons (yellow
E neuron) that
constrict the
extensor muscle,
causing the leg
to kick upward.
The stretch reflex
is a
monosynaptic
reflex. ‘Stretch
Reflex Extensor’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
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International
License.
The sensory neurons also synapse on interneurons within the spinal cord that are inhibitory (this
means that these neurons make and release GABA). These inhibitory interneurons then synapse on
alpha motor neurons that innervate the hamstring, the antagonistic flexor muscle to the quadriceps.
When these motor neurons are inhibited, the hamstring muscle relaxes, allowing the contraction of the
quadriceps to occur with more ease. This is called reciprocal inhibition.
Figure 41.2. In
addition to the
monosynaptic
extensor reflex,
the sensory
information from
the muscle
spindle sensory
cell (blue S
neuron) also
activates
inhibitory
interneurons
(black – neuron)
in the spinal cord.
These
interneurons
then inhibit the
motor neurons
(orange F
neuron) that
innervate the
flexor muscle,
causing the
flexor muscle to
relax. This
relaxation allows
the extensor
muscle to kick
the leg up with
less opposition
from the flexor
muscle. ‘Stretch
Reflex’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
International
License.
Withdrawal (Flexor) Reflex

A similar process can be seen in the withdrawal reflex. In this case, instead of an extension, the
muscles lead to muscle flexion in response to a stimulus. If, for example, you step on something
painful, the reflex will be to lift the injured foot. The sensory information that initiates this reflex is
activation of pain receptors, or nociceptors. Like with the stretch reflex, the sensory information enters
the spinal cord at the dorsal horn. Unlike the stretch reflex, the withdrawal reflex is a polysynaptic
reflex, meaning interneurons are present between the sensory neurons and the motor neurons.
Excitatory interneurons communicate with the alpha motor neurons of the flexor muscle, whereas
inhibitory interneurons communicate with the alpha motor neurons of the extensor muscle. The
behavioral response is flexing of the leg upward (the opposite action of the stretch reflex).
Figure 41.3. Pain

information is
sent from the
periphery to the
spinal cord via a
nociceptor
receptor cell (blue
S neuron in
dorsal root
ganglion). The A
delta sensory
axons synapse
on interneurons
within the spinal
cord. Excitatory
interneurons
(green + neuron)
activate motor
neurons (orange
F neuron) that
constrict the
flexor muscle.
Inhibitory
interneurons
(black – neuron)
inhibit motor
neurons (yellow
E neuron) that
innervate and
relax the
extensor muscle.
The leg lifts in
response.
‘Withdrawal
Reflex’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Crossed-Extensor Reflex
Running in parallel to the withdrawal reflex is the crossed-extensor reflex. If you step on something
sharp and lift that leg, your other leg needs to be able to support your weight shift, or you would fall.
This is accomplished by interneurons that cross the midline of the spinal cord and communicate with
motor neurons on the contralateral side of the body. The painful sensory information that initiated the
withdrawal reflex also initiates the crossed-extensor reflex. In addition to the ipsilateral interneurons
active in the withdrawal reflex, the sensory axons also synapse on excitatory interneurons that cross
the midline. These interneurons then synapse on excitatory interneurons that activate the alpha motor
neurons of the extensor muscle and inhibitory interneurons that inhibit the alpha motor neurons of
the flexor muscle (the opposite configuration to the withdrawal reflex). This leads to the leg extending,
providing a stable base for the weight shift.
Figure 41.4. If the

leg lifts due to
the withdrawal
reflex, the
opposite leg
must stabilize via
contraction of
extensor muscles
to balance the
body. This is
accomplished by
excitatory spinal
interneurons that
cross the midline
and
communicate the
sensory
information to
the contralateral
side of spinal
cord. Inhibitory
interneurons
cause relaxation
of the
contralateral
flexor muscles,
and excitatory
interneurons
cause
constriction of
the contralateral
extensor
muscles.
‘Crossed-Extenso
r Reflex’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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License.
Key Takeaways
• Control of reflexes occurs within the spinal cord and input from the brain is not
needed
Test Yourself!
Attributions
Media Attributions
• Stretch Reflex Extensor © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Stretch Reflex © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Withdrawal Reflex © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
• Crossed Extensor Reflex © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
602 | CENTRAL PATTERN GENERATORS
42.
CENTRAL PATTERN GENERATORS
Central pattern generators (CPGs) are networks of cells

that are capable of producing intrinsic motor responses
even in the absence of sensory or brain inputs. These
Resources motor responses are usually well-rehearsed, repetitive, and
happen at the unconscious level, meaning that certain
behavioral outputs can be performed independently of
signals upstream in the motor cortex.
• Glossary Terms CPGs have been observed in several organisms, ranging
• Key takeaways from insects, crayfish, birds, and mammals including
• Test yourself! humans, hinting that their evolution was highly adaptive
for survival. Imagine how difficult it would be to survive if
every breath required a conscious thought! The CPG is
not a standalone driver of motor activity, however. The
circuits receive signals from higher brain areas which can modify their characteristics. Imagine that you
want to consciously hold your breath, or intentionally walk in some unusual or goofy way. In both
cases, the descending signals from the brain are able to override the output motor command pattern
that the CPG normally produces. Once you stop intentionally changing your motor behavior, the
muscles return to their normal activity in response to CPG output.
Central Pattern Generators in Different Species

Many earlier studies of spinal cord CPGs were conducted in different animal groups with unique
locomotor patterns. Mollusks have crawling activity that is rhythmic, fish swim by sending alternative
patterns of muscle activity through their body, and birds fly by rhythmic flapping of their wings.
In mammals, spinal CPGs were demonstrated in cats with a thoracic-level spinal cord transection.
Following this surgery, the signals from the brain were unable to be communicated down to the hind
CENTRAL PATTERN GENERATORS | 603
legs, eliminating voluntary movement. They exhibited weakened muscular power, but were still able to
stand for short times. If their front paws were put onto a stable platform while their hindlegs were put
on a moving treadmill, the cat would involuntarily walk to keep up.
Humans also have CPGs for movement. One remarkable study looked at 37-year-old man who
had injured his spinal cord in a football accident as a young man. His injury was at the level of C5,
resulting in complete paralysis and a loss of sensation from the neck down. Over the following years,
he gradually recovered a small amount of function. Fine manipulation skills and bladder bowel control
never recovered, and he had not walked unassisted for ten years, experiencing tremendous weakness
after taking a few steps. Just days after beginning a physical therapy regimen consisting of assisted
upright walking, upon lying down, the man reported alternating muscle flexion and relaxation of
the hips, knees, and ankles similar to the pattern of activity seen in locomotion. He was unable to
voluntarily stop these motions, and could only get some rest by turning to the side. This muscle activity
was smooth and rhythmic, as would be seen in a healthy person walking.
Locomotion
Some example motor responses driven by CPGs include diaphragm movement (respiration),
alternating leg swinging and foot flexing (walking), and the progressive contraction of up to 25 pairs of
muscles in the tongue and mouth (swallowing). Some CPGs are located in the brain stem (respiration)
and others throughout the spinal cord (locomotion). Locomotion is one example of a basic, rhythmic
movement that requires coordination of a number of muscle groups to work properly.
Figure 42.1.
Walking cycle of
a human. The
arms and legs
must be
coordinated in
opposing fashion
during
locomotion. The
gray hand and
foot are left side
limbs. When one
is in front of the
body, the other is
behind. ‘Walking
Cycle’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Activity of extensor and flexor muscles in both legs must be coordinated to allow smooth locomotion
without falling. These rhythmical movements are controlled at the level of the spinal cord by circuits
called central pattern generators. The spinal cord has circuitry that, in the case of walking, moves the
legs in opposite patterns. When one leg is lifting up to move forward, the other leg is stable, touching
the ground.
Figure 42.2.
While walking,
there must be
coordinated,
reciprocal
activation of the
extensor and
flexor muscles of
each leg; as an
extensor is
contracted (gray
bar) the flexor
must relax.
Additionally, the
muscle activation
of one leg must
be the opposite
of the other leg,
so the right
extensor and the
left flexor are
activated at the
same time.
‘Walking Cycle
Muscle
Activation’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Spinal Circuitry
The control of this system has multiple levels. Neurons themselves may have pacemaker properties
that allow for a continuous cycle of depolarization and repolarization. These neurons are then located
within multi-cell circuits involving a collection of excitatory and inhibitory interneurons that results in
reciprocal inhibition of contralateral muscles. Additional networks of spinal interneurons would cause
reciprocal inhibition of ipsilateral antagonistic muscles.
Figure 42.3.
Central pattern
generators are
controlled by
interneuron
circuitry within
the spinal cord.
The circuit would
require the motor
neurons on the
opposite side of
the spinal cord to
be activated in a
reciprocal
fashion. This is
accomplished
through a
network of
excitatory and
inhibitory
interneurons that
allow for the
flexor (or
extensor) muscle
on one side of
the body to
control while the
contralateral
muscle relaxes.
‘Central Pattern
Generator Circuit’
by Casey Henley
is licensed under
a Creative
Commons
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Although the spinal cord is able to control these movements on its own, there is input from both
the brainstem and sensory neurons which can have an effect on modulating the pattern of neuronal
activity in the spinal cord. For example, when an animal needs to slow down, speed up, or turn away
from a danger, for example, those inputs can alter the spinal cord circuit.
Key Takeaways
• Central pattern generators are circuits in the spinal cord that control repetitive,
consistent movements like walking
Test Yourself!
Attributions
Media Attributions
• WalkingCycle © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA

• Walking Cycle Muscle Activation © Casey Henley adapted by Valerie Hedges is licensed under a
CC BY-NC-SA (Attribution NonCommercial ShareAlike) license
PLANNING OF MOVEMENT | 609
43.
PLANNING OF MOVEMENT
All voluntary (or non-reflexive) movements begin as

signals in the brain. Specifically, the neurons involved in
motor control are primarily found in the frontal lobe of Resources
the telencephalon, which includes areas such as primary
motor cortex (or M1), premotor cortex, and supplemental
motor areas. The posterior parietal cortex also contributes
• Glossary Terms
to movement. Through this section, we will walk through
• Key Takeaways
the brain processes leading to voluntary motor action.
• Test Yourself
There are a number of steps that must take place for
voluntary movement to occur. Assessment of the
surrounding environment and the body’s location in
space, followed by determining what action is appropriate, and then initiating that action.
For example, after work, you sit down on the couch to watch one episode of your favorite show. As
the end credits appear, you realize it is now time to head to your study space and start working on class.
To do this, you need to leave the couch, grab your computer from the table, get your coffee from the
kitchen and head to a different room. All of these voluntary movements take a great deal of processing
by the brain. You must assess your surrounding environment and your body’s location in it, determine
which actions need to be completed, and then actually initiate those actions. In this chapter we will
focus on how the planning of voluntary movement occurs.
Cortical Anatomy
Much of the cortex is actually involved in the planning of voluntary movement. Sensory information,
particularly the dorsal stream of the visual and somatosensory pathways, are processed in the posterior
parietal lobe where visual, tactile, and proprioceptive information are integrated. The posterior
610 | PLANNING OF MOVEMENT
parietal cortex is largely concerned with integrating somatosensory with visual information and
determining an appropriate motor action. For example, if you were planning to get up from your seat
to walk across the room, the posterior parietal cortex would take in the somatosensory proprioceptive
information about how your body is positioned, and the visual information from the objects in the
room to avoid running into them (recall the dorsal stream pathway).
Figure 43.1.
Information from
multiple sensory
systems are
processed in the
posterior parietal
lobe. Projections
are sent from the
primary
somatosensory
and primary
visual cortices.
‘Posterior
Parietal by Casey
Henley is
licensed under a
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With respect to motor control, the prefrontal cortex (PFC), located in the front of the brain in the
frontal lobe initiates the long-term planning or cognitive aspects of movements. For example, consider
the motor actions related to brushing your teeth. PFC signals are more akin to “brushing is good for
my hygiene and health”, rather than “move my arm and open my mouth.” PFC also helps determine
if some motor action is appropriate for the specific situation. Think of a behavioral test where you
are given two clickers, one to hold in each hand. You are told that when the experimenter shows you
a green item, you should click with the right button. After repeating this behavior multiple times,
you are told to switch – now, when you see a green item, you need to click the left button. In this
experiment, PFC is responsible for deciding which motor pattern (left button or right button press) is
appropriate in response to the stimulus. PFC also works to weigh the consequences of motor actions
and makes updates about future motor actions in similar or different circumstances. The exact same
motor action produces different results depending on the specific situation, and PFC contributes to
evaluating and predicting outcomes.
The premotor area lies just anterior to the primary motor cortex (M1) (M1 is just anterior
to the central sulcus). The premotor area helps plan and organize movement and makes decisions
about which actions should be used for a situation. The premotor area modulates motor output, and
generally activates prior to motor activity.
Figure 43.2.
Sensory
information from
the posterior
parietal is
processed in the
prefrontal cortex
and premotor
area, both
located in the
frontal cortex.
These areas then
send information
to the primary
motor cortex
located in the
precentral gyrus.
‘Motor Regions’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
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BY-NC-SA) 4.0
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View the primary motor cortex using the BrainFacts.org 3D Brain

View the premotor cortex using the BrainFacts.org 3D Brain
Role of Premotor Area

The premotor regions do send some axons directly to lower motor neurons in the spinal cord using
the same pathways as the motor cortex (see Execution of Movement chapter). However, the premotor
cortex also plays an important role in the planning of movement. Two experimental designs have
demonstrated this role. Monkeys were trained on a panel that had one set of lights in a row on top and
one set of buttons that could also light up in a row on the bottom. The monkeys would watch for a top
row light to turn on. This would indicate that within a few seconds, the button directly below would
light up. When the button turned on, the monkeys were supposed to push the button.
Therefore, there were two light triggers in the experiment. The first required no motor movement
from the monkey but did give the monkey information about where a motor movement would be
needed in the near future. The second required the monkey to move to push the button. When brain
activity was measured during this study, neurons in the premotor cortex became active when the first
light trigger turned on, well before any movement actually took place (Weinrich and Wise, 1928).
Figure 43.3. (A)

Monkeys were
trained to use a
light panel.
When lit, a light
located in the top
row informed the
monkey that the
button directly
below would
light up soon.
Shortly after one
of the top row
lights turned on,
the button
directly below it
would turn on,
and the monkey
would need to
push that button
for a reward. (B)
When no lights
are lit, premotor
neurons fire at a
baseline rate, and
the monkey does
not move.
Neurons located
in the premotor
cortex increase
action potential
firing rate when
the top row light
turns on, even
though the
monkey makes
no movement.
The firing stops
shortly after the
monkey moves
to push the
bottom button
after it turns on.
(Based on
Weinrich and
Wise, 1928)
‘Light Panel
Experiment’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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In another experiment, people were trained to move their fingers in a specific pattern. Cerebral blood
flow was then measured when they repeated the finger pattern and when they only imagined repeating
the finger pattern. When the movement was only imagined and not actually executed, the premotor
regions along with parts of the prefrontal cortex were activated (Roland, et al, 1980).
Figure 43.4.
People were
trained to move
their fingers in a
pattern. When
the pattern was
repeated, brain
activity was seen
in the primary
motor cortex,
along with the
premotor area
and prefrontal
cortex. When the
pattern was only
imagined, and no
finger movement
took place, brain
activity was seen
in the premotor
area and
prefrontal cortex.
(Based on
Roland, et al,
1980) ‘Finger
Movement
Experiment’ by
Casey Henley is
licensed under a
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These studies show that the premotor cortex is active prior to the execution of movement, indicating
that it plays an important role in the planning of movement. The posterior parietal, prefrontal, and
premotor regions, though, also communicate with a subcortical region called the basal ganglia to fully
construct the movement plan. The basal ganglia are covered in a future chapter.
Mirror Neurons
There is debate about the existence of a special population of cells in the premotor area called mirror
neurons. These are cells that are active during a movement, but also when that same movement is
observed in another animal or person. Proponents argue that these neurons are involved in learning
behaviors and for understanding the behaviors of others.
Key Takeaways
• Sensory information is processed in the posterior parietal before being sent to motor
regions of the brain
• The prefrontal cortex and premotor cortex are critical for creating a movement plan
• Mirror neurons
Test Yourself!
References
Roland PE, Larsen B, Lassen NA, Skinhøj E. Supplementary motor area and other cortical
areas in organization of voluntary movements in man. J Neurophysiol. 1980 Jan;43(1):118-36.
doi: 10.1152/jn.1980.43.1.118. PMID: 7351547.
Weinrich M, Wise SP. The premotor cortex of the monkey. J Neurosci. 1982
Sep;2(9):1329-45. doi: 10.1523/JNEUROSCI.02-09-01329.1982. PMID: 7119878; PMCID:
PMC6564318.
Attributions
Media Attributions
• Posterior Parietal © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Motor Regions © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Light Panel Experiment © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Finger Movement Experiment © Casey Henley adapted by Valerie Hedges is licensed under a
618 | EXECUTION OF MOVEMENT
44.
EXECUTION OF MOVEMENT
Motor cortex
Resources
Once the plan for movement has been created, the
primary motor cortex is responsible for the execution of
that action. The primary motor cortex lies just anterior to
• Glossary Terms the primary somatosensory cortex in the precentral gyrus
• Scientist Links to learn located in the frontal lobe.
more Primary motor cortex (M1) is a major motor control
• Key Takeaways center, required for deliberate, voluntary movements,
• Test Yourself movements made in response to a “command.”
Furthermore, motor cortex cells influence motor neurons,
neurons located in the spinal cord that ultimately
communicate with muscles or glands. This connection is
so strong that the motor cortex cells are also called upper motor neurons. In this terminology, a lower
motor neuron is found at the brain stem or spinal cord, and fires whenever the upper motor neuron
sends a signal.
EXECUTION OF MOVEMENT | 619
Figure 44.1. The

primary motor
cortex is located
in the frontal
lobe in the
precentral gyrus,
just anterior to
the central
sulcus. ‘Primary
Motor Cortex’ by
Casey Henley is
licensed under a
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Like the somatosensory cortex, the motor cortex is organized by a somatotopic map. In the 1930s,
neurosurgeon Wilder Penfield conducted several brain operations to treat patients with severe
epilepsy. However, since the brain has no pain receptors, he was able to remove a portion of the skull
under local anesthesia while the patients were awake and responding to his questions. During surgery,
the goal was to electrically stimulate portions of the cortex to determine the origin of the seizures, and
to ensure that areas critical to speech and hand movement were left untouched so that the patient
would not have major impairments following surgery. This is currently done in neuro-oncology to
reduce the loss of critical motor function and overall morbidity. Penfield progressively moved across
different brain areas of M1 while using an electrode to stimulate patches of the cortex.
He had two major observations. First, stimulation caused contralateral activity: that is, stimulating
the left side of the brain affects the muscle activity of the right side of the body. Secondly, by
systematically moving across M1, he observed that different populations of neurons are responsible for
communicating with specific muscle groups. For instance, dorsal M1 activates hip and trunk muscles,
while more lateral M1 activates muscles of the face. Penfield discovered that within the motor cortex,
different muscle groups are laid out in a rough topography, meaning that neurons that control the
thumb are near other neurons that control the thumb, and near other populations of neurons that
control the index finger. A graphical representation of this map is called the “motor homunculus,” in
which body parts with larger representations in the brain are shown with larger size (much like the
sensory homunculus).
Interestingly, the motor cortex does not map onto the body in such an exact way as does the
somatosensory system. It is believed that upper motor neurons in the motor cortex control multiple
lower motor neurons in the spinal cord that innervate multiple muscles. This results in activation
of an upper motor neuron causing excitation or inhibition in different neurons at once, indicating
that the primary motor cortex is responsible for movements and not simply activation of one muscle.
Stimulation of motor neurons in monkeys can lead to complex motions like bringing the hand to the
mouth or moving into a defensive position (Graziano et al, 2005).
Figure 44.2. The

map of the body
that exists on the
motor cortex is
less specific than
the
somatosensory
map because
cortical neurons
control multiple
muscles at the
same time.
Instead, regions
of the cortex are
associated with
larger body
regions, such as
the face, arm and
hand, trunk, or
leg and foot.
‘Motor Cortex
Map’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Population coding
The motor cortex controls movement by using population coding mechanisms. Upper motor
neurons are broadly tuned to a certain movement in a certain direction, meaning firing rate is highest
when moving in one direction, but firing also occurs when moving in nearby directions. For example,
when a monkey is trained to move its hand toward the left, neurons “tuned” toward left movement will
be active immediately before and during the movement. Neurons tuned to other directions will also
be active but at lower rates (Georgopoulos, et al, 1982). This means that the firing rate of one specific
neuron does not give enough information to know direction of movement. It is the combined firing
rates of an entire population of neurons that indicates direction.
Figure 44.3.
Motor movement
is coded via
population
coding in the
primary motor
cortex.
Information from
one neuron is not
enough to
determine the
direction of
movement; a
population of
neurons must be
used. Some
neurons will be
“tuned” to fire
most rapidly in
response to a
specific direct.
For example,
Neuron 1 in the
figure shows the
highest firing
rate when
movement of the
hand is to the left
and a low firing
rate when the
hand is moving
to the right,
whereas Neuron
3 fires the most
when the
movement is
forward. The
combination of
the firing
patterns of many
neurons provides
a precise
direction for the
movement.
‘Population
Coding’ by Casey
Henley is
licensed under a
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Commons
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Descending Spinal Tracts

There are multiple descending tracts within the spinal cord that send information from the brain to the
motor neurons in the ventral horn. The lateral tracts are responsible for carrying information about
voluntary movement of the arms and legs. The ventromedial pathways are responsible for carrying
information about posture and balance.
Figure 44.4. The

descending
motor tracts
travel from the
brain through the
white matter in
the spinal cord.
The lateral tracts
descend in the
dorsolateral
white matter,
and the
ventromedial
tracts descend in
the ventromedial
white matter.
‘Descending
Spinal Tracts’ by
Casey Henley is
licensed under a
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Lateral Tracts
The lateral pathways allow for voluntary movement of muscles and are under direct cortical control.
Corticospinal Tract
The largest of the lateral pathways is the corticospinal tract. This pathway sends information directly
from the motor and premotor cortices down to the motor neurons in the spinal cord. Cortical
axons travel through the brainstem and then cross the midline at the base of the medulla; like the
somatosensory system, the right side of the cortex processes information for the left side of the body
and vice versa. In the spinal cord, the axons travel through the lateral column and synapse in the ventral
horn on motor neurons that typically innervate distal muscles.
Figure 44.5.
Motor
information to
the arms and
legs travels from
the primary
motor cortex to
the medulla via
the internal
capsule, a white
matter structure
in the brain. The
corticospinal
tract passes
through the
medullary
pyramids and
then decussates
in the caudal
medulla. The
axons continue
traveling through
the lateral
corticospinal
tract and
synapse on an
alpha motor
neurons in the
ventral horn of
the spinal cord.
‘Corticospinal
Tract’ by Casey
Henley is
licensed under a
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Commons
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Corticobulbar Tract
The corticobulbar tract is another lateral tract and sends motor information to cranial nerves for
motor control of the face. This path travels ipsilateral from the cortex into the brainstem where it
branches off at the appropriate cranial nerve level in either the pons or the medulla and then innervates
cranial nerve neurons bilaterally.
Figure 44.6.
Motor
information to
the face travels
from the primary
motor cortex
through the
internal capsule
to the pons and
medulla where it
branches to
synapse on
cranial nerve
nuclei on both
sides of the
brainstem.
‘Corticobulbar
Tract’ by Casey
Henley is
licensed under a
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Commons
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Ventromedial Tracts
There are four ventromedial pathways that travel in the spinal cord as well. These tracts begin in the
brainstem and descend through the ventromedial columns. They receive input from motor areas of the
cortex as well as integrating information from multiple sensory regions. The ventromedial pathways
control posture and locomotion.
• The vestibulospinal tract is important for head balance as we move. This tract begins in the
vestibular nucleus. The vestibular nucleus processes information from the vestibular apparatus
(semicircular canals). The axons of the vestibular nucleus project down the cervical spinal cord
to control neck and back muscles and guide head movements and also project to the lumbar
spinal cord to maintain an upright posture.
• The tectospinal tract is responsible for moving the head in response to visual stimuli. This
tract begins in the superior colliculus (of the tectum). The axons of the superior colliculus
decussate and project into the cervical regions of the spinal cord to control muscles of the neck,
upper trunk, and shoulders.
• The two reticulospinal tracts play a role in managing anti-gravity reflexes needed for posture
and standing. These tracts begin in the reticular formation.
Key Takeaways
• The motor cortex is located in the frontal lobe

• The motor map is not as detailed as the somatosensory homunculus
• The motor cortex uses population coding to encode direction of movement
• The lateral tracts carry information about voluntary movement of the arms and legs
• The ventromedial pathways carry information about posture and balance
Test Yourself!
References
Georgopoulos AP, Kalaska JF, Caminiti R, Massey JT. J Neurosci. 1982 Nov;2(11):1527-37.
Graziano et al, 2005 J Neurophysiol 94: 4209-4223.
Attributions
Media Attributions
• Primary Motor Cortex © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Motor Cortex Map © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
• Population Coding © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
• Descending Spinal Tracts © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Corticospinal Tract © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
• Corticobulbar Tract © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
BASAL GANGLIA | 631
45.
BASAL GANGLIA
The basal ganglia are a group of subcortical nuclei,

meaning groups of neurons that lie below the cerebral
cortex. The basal ganglia is comprised of the striatum, Resources
which consists of the caudate nucleus and the putamen,
the globus pallidus, the subthalamic nucleus, and the
substantia nigra The basal ganglia are intimately
• Glossary terms
involved with various aspects of movement, such as
• Key Takeaways
voluntary motor activity, habit learning, and the selection
• Test Yourself
of actions.
For voluntary motor behavior, the basal ganglia are
involved in the initiation or suppression of behavior and
can regulate movement through modulating activity in the thalamus and cortex. Despite being major
structures involved in motor functions, none of the components of the basal ganglia directly send
projections down the spinal cord. Rather, they communicate mostly within themselves before
signaling through the motor cortex. While the connections between basal ganglia structures have been
largely mapped out, the specific functions of each individual structure in isolation is not entirely clear.
Diseases of the basal ganglia include movement disorders such as Parkinson’s disease, dystonia,
Huntington’s disease, and Tourette’s, as well as complex psychiatric disorders including addiction and
obsessive-compulsive disorder (OCD).
Basal Ganglia Structures- Introduction

As we discuss the motor circuitry of the basal ganglia, it is important to understand that it is a loop.
Activity from the cortex feeds into the basal ganglia, then through the thalamus, and then back to the
cortex. The function of this loop of structures is to ultimately select and initiate willed movements.
632 | BASAL GANGLIA
The structures of the basal ganglia are briefly defined below.

Striatum– The striatum is made up of two components, the caudate nucleus and putamen. On a
cellular level, the majority of neurons in the striatum are GABAergic cells.
Globus Pallidus– In dissection, the globus pallidus appears as a pale globe. It is subdivided into two
components with functionally different connections. The globus pallidus internal segment (GPi) is
located more medially and is movement promoting. On the other hand, the globus pallidus external
segment (GPe) is located more laterally and is movement inhibiting. The output of the globus pallidus
is only via inhibitory projections that are tonically active. This means that in the absence of any input,
globus pallidus neurons will still inhibit their targets.
Subthalamic nucleus– The subthalamic nucleus is an output of the globus pallidus external
segment. The output of the subthalamic nucleus are glutamatergic signals into the globus pallidus
internal segment.
Substantia Nigra– The substantia nigra is a midbrain structure that appears darker in dissection
due to heavy expression of neuromelanin across cells in these areas. It is the largest midbrain structure.
The substantia nigra contains several dopamine expressing neurons that project into the striatum. Of
clinical significance, these neurons experience selective neurodegeneration in Parkinson’s disease.
BASAL GANGLIA | 633
Figure 45.1. The

basal ganglia are
subcortical
structures
located at the
base of the
forebrain. They
are comprised of
the caudate and
putamen, which
both make up
the striatum, as
well as the
globus pallidus,
substantia nigra,
and subthalamic
nucleus. ‘Basal
Ganglia’ by Casey
Henley is
licensed under a
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Commons
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View the basal ganglia using the BrainFacts.org 3D Brain
Basal Ganglia Input

The dorsal striatum receives both glutamatergic and dopaminergic inputs. The majority of
information processed by the basal ganglia enters through the striatum, with projections coming from
both cortical and limbic structures such as thalamus and amygdala. This input into the striatum
is glutamatergic and therefore, excitatory. The striatum also receives dopaminergic projections from
another basal ganglia structure, the substantia nigra, a communication route called the nigrostriatal
pathway.
634 | BASAL GANGLIA
Dopamine plays an important role in basal ganglia function. Parkinson’s disease results when
dopamine neurons in the substantia nigra degenerate and no longer send appropriate inputs to the
striatum. Dopamine projections can have either excitatory or inhibitory effects in the striatum,
depending on the type of metabotropic dopamine receptor the striatal neuron expresses. Dopamine
action at a neuron that expresses the D1 receptor is excitatory. Dopamine action at a neuron that
expresses the D2 receptor is inhibitory.
Figure 45.2.
Inputs to the
basal ganglia
enter through
the striatum (the
caudate and
putamen).
Cortical
projections
(shown in green)
release
glutamate and
are excitatory.
Substantia nigra
projections
(shown in blue)
release dopamine
and can be either
excitatory or
inhibitory. ‘Basal
Ganglia Input’ by
Casey Henley is
licensed under a
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Commons
Attribution
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BASAL GANGLIA | 635
Figure 45.3. The

cortex sends
glutamate
projections to the
striatum. The
substantia nigra
sends dopamine
projections to the
striatum. ‘Basal
Ganglia Input –
Text’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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License.
Basal Ganglia Output

The primary output region of the basal ganglia is the internal segment of the globus pallidus (GPi).
This region sends inhibitory GABAergic projections to nuclei in the thalamus. This inhibitory output
has a tonic, constant firing rate, which allows the basal ganglia output to both increase and decrease
depending on the situation. The thalamus then projects back out to the cerebral cortex, primarily to
motor areas.
636 | BASAL GANGLIA
Figure 45.4.
Output from the
basal ganglia
leaves through
the internal
segment of the
globus pallidus.
Inhibitory
projections
(shown in red)
release GABA
onto the
thalamus.
Excitatory
thalamic
projections
(shown in green)
communicate
with the cerebral
cortex. ‘Basal
Ganglia Output’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
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BASAL GANGLIA | 637
Figure 45.5. The

internal segment
of the globus
pallidus sends
GABA projections
to the thalamus.
The thalamus
sends glutamate
projections to the
cortex. ‘Basal
Ganglia Output –
Text’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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License.
Basal Ganglia Internal Processing
Direct Pathway
There are multiple connections within the basal ganglia structures as well. For motor control, there
are two main circuits: the direct pathway and the indirect pathway. These circuits have opposing
actions when activated by cortical neurons. The circuits are also modulated by dopamine release by the
substantia nigra into the striatum. It is believed that the different control mechanisms allow a finely
tuned balance between the direct and indirect circuits, which allows for refined control of movement.
The direct pathway begins with excitatory input from the cortex to the striatum (caudate and
putamen). The striatum then sends inhibitory projections to the internal segment of the globus
pallidus (GPi). The GPi then sends inhibitory output to the thalamus. The direct pathway initiates
and facilitates voluntary movement.
638 | BASAL GANGLIA
Figure 45.6. The

direct pathway in
the basal ganglia
consists of
excitatory input
from the cortex
via glutamate
action or
substantia nigra
via dopamine
action that
synapses on
inhibitory
neurons in the
striatum. The
striatal neurons
project to the
internal segment
of the globus
pallidus (GPi).
The GPi then
sends inhibitory
output to the
thalamus. ‘Basal
Ganglia Direct
Pathway’ by
Casey Henley is
licensed under a
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Attribution
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BASAL GANGLIA | 639
Figure 45.7. The

cortex sends
glutamate
projections to the
striatum. The
substantia nigra
sends dopamine
projections to the
striatum, which
are excitatory,
acting on D1
receptors in the
neurons involved
in the direct
pathway. The
striatum sends
GABA projections
to the internal
segment of the
globus pallidus
(GPi). The GPi
sends GABA
projections to the
thalamus. The
thalamus sends
glutamate
projections to the
cortex. ‘Basal
Ganglia Direct
Pathway – Text’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
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BY-NC-SA) 4.0
International
License.
Activation of the Direct Pathway
To make a movement, there needs to be activation of the direct pathway.

640 | BASAL GANGLIA
When input from either the cortex or substantia nigra increases in intensity, the direct pathway is
activated. The neurons in the striatum involved in the direct pathway express the D1 metabotropic
dopamine receptor, and the activation of this receptor is excitatory. Therefore, projections from both
the cortex and the substantia nigra activate the neurons in the striatum. The striatal neurons are
inhibitory and release GABA onto the internal segment of the globus pallidus (GPi). As described
above, the neurons in the GPi are inhibitory, releasing GABA onto the thalamus. Activation of the
striatum neurons causes the release of GABA onto neurons in the GPi, inhibiting the GPi neurons.
Inhibiting the GPi neurons releases their typical inhibition on the thalamus. Inhibition of an
inhibitory region is called disinhibition. Therefore, the activation of the direct pathway results in
increased output from the thalamus because it is disinhibited. Increased thalamic activity leads to
increased cortical activity, and increased movement.
When at rest (not moving), there is not an increase in either glutamatergic or dopaminergic input
into the striatum. As a result, there is no change in striatal activity to modulate activity of the GPi.
The GPi will continue to tonically inhibit the thalamus, keeping cortical activity low and decreasing
movement.
BASAL GANGLIA | 641
Figure 45.8.
Activation of the
direct pathway
by either
increased input
from either the
cortex or
substantia nigra
leads to
increased
inhibitory output
from the
striatium to the
GPi. The
inhibition on the
GPi leads to less
inhibitory input
to the thalamus,
causing increased
output from the
thalamus to the
cortex. ‘Direct
Pathway
Activation’ by
Casey Henley is
licensed under a
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Commons
Attribution
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642 | BASAL GANGLIA
Figure 45.9.
When either the
cortex or the
substantia nigra
are activated,
they send
increased
excitatory output
to the striatum,
which expresses
excitatory D1
receptors in the
neurons involved
in the direct
pathway. This
input activates
the striatum,
which sends
increased
inhibitory
projections to the
GPi. The inhibited
GPi sends
decreased
inhibitory
projections to the
thalamus,
disinhibiting the
thalamus. The
thalamus then
sends increased
excitatory output
to the cortex.
‘Direct Pathway
Activation – Text’
by Casey Henley
is licensed under
a Creative
Commons
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BASAL GANGLIA | 643
Indirect pathway
The indirect pathway is a little more complex. Like the direct pathway, input into the basal ganglia
arises from the cortex and substantia nigra, but there are more internal connections within the basal
ganglia than what occurs in the direct pathway. Inhibitory neurons in the striatum involved in the
indirect pathway project to the external segment of the globus pallidus (GPe). GABA-ergic neurons
in the GPe project to the subthalamic nucleus, which then sends excitatory output to the GPi, which
outputs to the thalamus.
644 | BASAL GANGLIA
Figure 45.10. The

indirect pathway
in the basal
ganglia consists
of excitatory
input from the
cortex via
glutamate action
or inhibitory
input from the
substantia nigra
via dopamine
action that
synapses on
inhibitory
neurons in the
striatum. The
striatal neurons
project to the
external segment
of the globus
pallidus (GPe).
The GPe the
sends inhibitory
output to the
subthalamic
nucleus, which
had excitatory
projections to the
GPi. The GPi then
sends inhibitory
output to the
thalamus. ‘Basal
Ganglia Indirect
Pathway’ by
Casey Henley is
licensed under a
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Commons
Attribution
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BASAL GANGLIA | 645
646 | BASAL GANGLIA
Figure 45.11. The

cortex sends
glutamate
projections to the
striatum. The
substantia nigra
sends dopamine
projections to the
striatum, which
are inhibitory,
acting on D2
receptors in the
neurons involved
in the indirect
pathway. The
striatum sends
GABA projections
to the external
segment of the
globus pallidus
(GPe). The GPe
sends GABA
projections to the
subthalamic
nucleus. The
subthalamic
nucleus sends
glutamate
projections to the
GPi. The GPi
send GABA
projections to the
thalamus. The
thalamus sends
glutamate
projections to the
cortex. ‘Basal
Ganglia Indirect
Pathway – Text’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
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Activation of the Indirect Pathway
The indirect pathway is activated by excitatory cortical input, activating the inhibitory striatal
neurons. This leads to inhibition of the GPe neurons, resulting in disinhibition of the excitatory
neurons in the subthalamic nucleus. The excitatory output from the subthalamic nucleus to the GPi
increases inhibition of the thalamus, leading to decreased thalamic output to the cortex.
648 | BASAL GANGLIA
Figure 45.12.
Activation of the
indirect pathway
by excitatory
cortical input to
the striatum
leads to
increased
inhibitory output
to the GPe. The
inhibited GPe
sends decreased
inhibitory output
to the
subthalamic
nucleus, causing
increased
excitatory output
from the
subthalamic
nucleus to the
GPi. Activation of
the GPi inhibits
the thalamus,
resulting in
decreased output
from the
thalamus to the
cortex. ‘Indirect
Pathway
Activation’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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License.
BASAL GANGLIA | 649
Figure 45.13.
When the cortex
is activated, it
sends increased
excitatory output
to the striatum.
This input
activates the
striatum, which
sends increased
inhibitory
projections to the
GPe. The
inhibited GPe
sends decreased
inhibitory
projections to the
subthalamic
nucleus,
disinhibiting the
region. The
subthalamic
nucleus then
sends increased
excitatory output
to the GPi. The
activated GPi
sends increased
inhibitory
projections to the
thalamus, which
sends decreased
excitatory output
to the cortex.
‘Indirect Pathway
Activation – Text’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
650 | BASAL GANGLIA
License.
Inhibition of the Indirect Pathway
The indirect pathway can be inhibited by dopamine release from the substantia nigra. The neurons in
the striatum involved in the indirect pathway express the D2 metabotropic dopamine receptor. The
activation of this receptor is inhibitory. If the indirect pathway is inhibited by dopamine projections
from the substantia nigra, the inhibitory striatal neurons are inhibited. This leads to disinhibiton
of the GPe neurons, resulting in inhibition of the excitatory neurons in the subthalamic nucleus.
This decreased excitatory output to the GPi decreases inhibition of the thalamus, leading to increased
thalamic output to the cortex.
BASAL GANGLIA | 651
Figure 45.14.
Inhibition of the
indirect pathway
by inhibitory
input from the
substantia nigra
to the striatum
leads to
decreased
inhibitory output
to the GPe. The
disinhibited GPe
sends increased
inhibitory output
to the
subthalamic
nucleus, causing
decreased
excitatory output
from the
subthalamic
nucleus to the
GPi. A decrease
in activation of
the GPi releases
the inhibition on
the thalamus,
resulting in
increased output
from the
thalamus to the
cortex. ‘Indirect
Pathway
Inhibition’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
652 | BASAL GANGLIA
BASAL GANGLIA | 653
Figure 45.15.
When the
substantia nigra
is activated, it
sends increased
inhibitory output
to the striatum,
which expresses
inhibitory D2
receptors in the
neurons involved
in the indirect
pathway. This
input inhibits the
striatum, which
sends decreased
inhibitory
projections to the
GPe. The
disinhibited GPe
sends increased
inhibitory
projections to the
subthalamic
nucleus,
inhibiting the
region. The
subthalamic
nucleus then
sends decreased
excitatory output
to the GPi. The
deactivated GPi
sends decreased
inhibitory
projections to the
thalamus, which
sends increased
excitatory output
to the cortex.
‘Indirect Pathway
inhibition – Text’
by Casey Henley
is licensed under
a Creative
654 | BASAL GANGLIA
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Summary of Internal Processing

To put it all together, there is input to the striatum from two different locations: cortex (glutamate)
and substantia nigra (dopamine).
• Cortical activation of the direct pathway leads to increased thalamic output

• Cortical activation of the indirect pathway leads to decreased thalamic output
• Substantia nigra activation (via D1) of the direct pathway leads to increased thalamic output
• Substantia nigra inhibition (via D2) of the indirect pathway leads to increased thalamic output
It is the combination of these pathways that allows for precise control of motor movement, balancing
the excitatory direct pathway with the inhibitory indirect pathway.
BASAL GANGLIA | 655
Figure 45.16.
Basal ganglia
circuit model. The
cortex sends
glutamate
projections to the
striatum. The
substantia nigra
sends dopamine
projections to the
striatum, which
are excitatory,
acting on D1
receptors in the
neurons involved
in the direct
pathway and D2
receptors in the
neurons involved
in the indirect
pathway. The
striatum sends
GABA projections
to the external
segment of the
globus pallidus
(GPe). The GPe
sends GABA
projections to the
subthalamic
nucleus. The
subthalamic
nucleus sends
glutamate
projections to the
GPi. The GPi
sends GABA
projections to the
thalamus. The
thalamus sends
glutamate
projections to the
cortex. ‘Basal
ganglia circuit
model’ by Valerie
656 | BASAL GANGLIA
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Loops through the Basal Ganglia

There are multiple circuits that pass through the basal ganglia:
• The motor circuit, which plays a role in voluntary movement

• The oculomotor circuit, which plays a role in eye movement
• The associative circuit, which plays a role in executive functions like behavioral inhibition
(preventing impulsive behaviors) planning and problem solving, and mediating socially
appropriate behaviors
• The limbic or emotional circuit, which plays a role in the processing of emotion and reward.
Although the circuits each use different circuits within the basal ganglia, the general loop is the same:
cortical input to the striatum leads to internal processing within the basal ganglia structures. Basal
ganglia output projects from the pallidum to the thalamus, which then projects back to the cortex.
It is important to recognize that the basal ganglia plays an important role in a number of functions.
For example, medications that are used to treat Parkinson’s can sometimes lead to the presentation
of impulse control disorders, a result of dopaminergic changes in the limbic loop through the basal
ganglia.
BASAL GANGLIA | 657
Figure 45.17.
Loops through
the basal ganglia
have different
functions but
follow the same
general circuit.
The cortex inputs
to the striatum.
Internal
processing
through basal
ganglia circuits
occurs, and then
the output from
the pallidum
projects to the
thalamus, which
sends output to
the cortex. ‘Basal
Ganglia Loops’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Key Takeaways
• The subcortical basal ganglia nuclei receive information from the cortex and send
output to the thalamus
658 | BASAL GANGLIA
• Motor control through the basal ganglia occurs through both the direct and indirect
pathways
• Disinhibition is when an inhibitory region is itself inhibited
• The basal ganglia are best known for their role in motor control but are also critical
for emotion and behavioral inhibition
Test Yourself!
Attributions
Media Attributions
• Basal Ganglia © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Basal Ganglia Input © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
BASAL GANGLIA | 659

• Basal Ganglia Input Text © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Basal Ganglia Output © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Basal Ganglia Output Text © Casey Henley adapted by Valerie Hedges is licensed under a CC
• Basal Ganglia Direct Pathway © Casey Henley adapted by Valerie Hedges is licensed under a CC
• Basal Ganglia Direct Pathway Text © Casey Henley adapted by Valerie Hedges is licensed under
a CC BY-NC-SA (Attribution NonCommercial ShareAlike) license
• Direct Pathway Activation © Casey Henley adapted by Valerie Hedges is licensed under a CC
• Direct Pathway Activation Text © Casey Henley adapted by Valerie Hedges is licensed under a
• Basal Ganglia Indirect Pathway © Casey Henley adapted by Valerie Hedges is licensed under a
• Basal Ganglia Indirect Pathway Text © Casey Henley adapted by Valerie Hedges is licensed
under a CC BY-NC-SA (Attribution NonCommercial ShareAlike) license
• Indirect Pathway Activation © Casey Henley adapted by Valerie Hedges is licensed under a CC
• IndirectPathwayActivationText_lecture © Casey Henley adapted by Valerie Hedges is licensed
under a CC BY-NC-SA (Attribution NonCommercial ShareAlike) license
• Indirect Pathway Inhibition © Casey Henley adapted by Valerie Hedges is licensed under a CC
• Indirect Pathway Inhibition Text © Casey Henley adapted by Valerie Hedges is licensed under a
• Basal Ganglia diagram © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Basal Ganglia Loops © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
660 | NEURODEGENERATIVE DISEASES- MOTOR
46.
NEURODEGENERATIVE DISEASES-
MOTOR
Neurodegenerative diseases cause neurons to lose either

their function or structure over time, potentially leading
to cell death in either the central or peripheral nervous
Resources system. These diseases can be devastating because there are
no known ways to reverse the neurodegeneration. There
are multiple diseases that are classified as
neurodegenerative diseases and affect millions of people
• Glossary terms worldwide. We will focus on the two most prevalent
• Key Takeaways neurodegenerative diseases: The motor disease
Parkinson’s disease that affects the basal ganglia, and
Alzheimer’s disease.
Parkinson’s Disease
Symptoms and Risk Factors

Parkinson’s disease is a neurodegenerative movement disorder that causes bradykinesia (slowness of
movement), akinesia (difficulty initiating movement), a resting tremor, muscle rigidity, and changes to
posture and locomotion. Although most symptoms are motor, there are mild cognitive and psychiatric
changes, such as apathy, anhedonia, mood disturbances, or depression.
NEURODEGENERATIVE DISEASES- MOTOR | 661
Figure 46.1.
Parkinson’s
disease. This
drawing depicts
the stooped
posture and
shuffling gait
that are both
symptoms of
Parkinson’s
disease.
Advanced age is the primary risk factor, as an estimated 2% of people over the age of 60 develop
Parkinson’s disease. It is also shows a sex difference with men being diagnosed more than women.
Other environmental factors contribute to risk, such as repeated traumatic brain injury (suspected in
Muhammad Ali) or occupational exposure to heavy metals, insecticides, or other neurotoxins. A small
percentage of cases are early onset (21 – 50 years old; Michael J. Fox was diagnosed at 30), and have
a strong genetic component. The disease causes significant decreases in life expectancy and quality of
life.
Basal Ganglia Dysfunction

A loss of dopaminergic neurons within the substantia nigra (a midbrain structure) contributes to
basal ganglia circuitry disruption in Parkinson’s disease. This loss of substantia nigra cells can be
visualized postmortem without the use of histology due to the natural black pigmentation of the
substantia nigra cells.
Figure 46.2.
Substantia nigra.
The substantia
nigra is a
midbrain
structure made
up of
dopaminergic
neurons that
project to the
striatum. These
dopaminergic
cells are naturally
pigmented black
(substantia =
substance, nigra
= black). In
Parkinson’s
disease, these
dopaminergic
cells die, and a
loss of the black
pigmented cells
can be observed
in human
cadavers.
When the substantia nigra cells die, they are no longer able to release dopamine at the striatum
appropriately. When less dopamine is released into the striatum, the two populations of striatal
neurons are affected differently dependent on the type of dopamine receptor that they express. Recall
the basal ganglia circuitry from the previous chapter.
Direct Pathway Dysfunction
Within the direct pathway, striatal cells (within the caudate) express excitatory D1 receptors that
project directly to the globus pallidus internal segment. In the direct pathway, typically, dopamine
binds to D1 receptors on striatal neurons, increasing caudate activity. These striatal neurons release
GABA onto the globus pallidus internal segment (GPi) neurons, causing the GPi neurons to be less
active. When the Gpi neurons are less active, they release less GABA onto the thalamic neurons,
increasing thalamic neuron activity and the excitatory projections to the motor cortex, thus increasing
movement.
In Parkinson’s disease, there is decreased release of dopamine onto the striatal caudate neurons that
express D1 receptors, making them less active. Now that the striatal neurons are not as active, they
release less GABA onto GPi neurons, disinhibiting them. The GPi is now more active, releasing more
GABA onto thalamic neurons. In response, the thalamic neurons are less active and send less excitatory
messages to the cortex, thus causing a decrease in movement overall.
Figure 46.3.
Changes in D1
Receptor activity
in Parkinson’s
disease. A. In
healthy
individuals, the
substantia nigra
releases
dopamine onto
striatal cells in
the caudate. The
caudate
expresses D1
receptors that
are excitatory.
When dopamine
binds to D1
receptor it causes
EPSPs in the
caudate neurons,
and an increase
in activity of the
caudate neuron.
B. The striatal
neurons are
GABAergic, so
when activity is
increased in
healthy
individuals, they
release more
GABA onto GPi
neurons. GABA
binds to
receptors on the
GPi neurons,
causing an influx
of Cl- ions and
membrane
hyperpolarization
, leading to less
activity. C. In
individuals with
Parkinson’s
disease, the
dopaminergic
substantia nigra
cells die, and as a
result do not
release dopamine
onto caudate
neurons. This
causes a
decrease in
activity of the
caudate neurons.
D. Decreased
activity of the
caudate neurons
leads to
decreased
release of GABA
onto the GPi
neurons. As a
result, the GPi
neurons have
increased
activity. ‘Changes
in D1 Receptors
activity in
Parkinson’s
disease’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Figure 46.4.
Model of Basal
Ganglia Circuitry
in Parkinson’s
disease- Direct
Pathway. This
model shows the
dopaminergic,
glutamatergic,
and GABAergic
connections
within the basal
ganglia circuitry.
Tonically active
connections are
indicated. The
direct pathway is
highlighted with
orange. Larger
arrows indicate
increased
activity, and
smaller arrow
indicated
decreased
activity. The
cortex provides
glutamatergic
input to the
striatum, which
also receives
dopaminergic
projections from
the substantia
nigra. In
Parkinson’s
disease, the
dopaminergic
neurons die and
there is a loss of
dopaminergic
input into the
striatum. As a
result, the
decreased D1
receptor
activation in the
caudate causes
the caudate cells
to be less active,
releasing less
GABA onto the
globus pallidus
internal segment.
The globus
pallidus internal
segment is
disinhibited, and
thus increases its
firing rate,
releasing more
GABA onto the
thalamus. The
decreased D2
receptor
activation in the
putamen causes
the putamen
cells to be more
active, releasing
more GABA onto
the globus
pallidus external
segment. The
globus pallidus
releases less
GABA onto the
subthalamic
nucleus. The
subthalamic
nucleus is
disinhibited, and
increases its
release of
glutamate onto
the GPi. This
causes the GPi to
increase its firing
rate, releasing
more GABA onto
the thalamus.
Together, the
decreased
activity of the
thalamus
decreases activity
of the motor
cortex and thus
decreases
movement.
‘Model of Basal
ganglia circuitry
in Parkinson’s
disease- direct
pathway’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Indirect Pathway Dysfunction
In the indirect pathway, dopamine typically binds to inhibitory D2 receptors on striatal (putamen)
neurons, inhibiting striatal neuron activity. These striatal neurons then release less GABA onto the
globus pallidus external segment neurons (GPe), causing the GPe neurons to be more active. When
the GPe neurons are more active, they release more GABA onto the subthalamic nucleus neurons,
decreasing subthalamic neuron activity and the excitatory projections to the GPi. Decreasing
activation of the GPi causes the GPi to release less GABA onto the thalamus, which then causes more
activation of the motor cortex, thus increasing movement.
In Parkinson’s disease, there is decreased release of dopamine onto the striatal neurons that express
D2 receptors, making them more active. Now that the striatal neurons are more active, they release
more GABA onto GPe neurons. This inhibits the GPe neurons, causing them to release less GABA
onto subthalamic nucleus neurons, disinhibiting the subthalamic nucleus. Increasing the excitatory
inputs from the subthalamic nucleus onto the GPi will cause the GPi to release more GABA onto
the thalamus. In response, the thalamic neurons are less active and send less excitatory messages to the
cortex, thus causing a decrease in movement overall.
Figure 46.5.
Parkinson’s
disease effect on
indirect pathway.
Changes in D2
Receptor activity
in Parkinson’s
disease. A. In
healthy
individuals, the
substantia nigra
releases
dopamine onto
striatal cells in
the putamen.
The putamen
expresses D2
receptors that
are inhibitory.
When dopamine
binds to D2
receptor it causes
IPSPs in the
putamen
neurons, and an
decrease in
activity of the
putamen neuron.
B. The striatal
neurons are
GABAergic, so
when activity is
decreased in
healthy
individuals, they
release less
GABA onto GPe
neurons.
Decreased
activity of the
caudate neurons
leads to
decreased
release of GABA
onto the GPi
neurons. As a
result, the GPe
neurons have
increased
activity. C. In
individuals with
Parkinson’s
disease, the
dopaminergic
substantia nigra
cells die, and as a
result do not
release dopamine
onto putamen
neurons. This
causes an
increase in
activity of the
putamen
neurons. D.
Increased activity
of the putamen
neurons leads to
increased release
of GABA onto
the GPe neurons.
As a result, the
GPe neurons
have decreased
activity. ‘Changes
in D2 Receptors
activity in
Parkinson’s
disease’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Figure 46.6.
Model of Basal
Ganglia Circuitry
in Parkinson’s
disease- Indirect
Pathway. This
model shows the
dopaminergic,
glutamatergic,
and GABAergic
connections
within the basal
ganglia circuitry.
Tonically active
connections are
indicated. The
indirect pathway
is highlighted
with orange.
Larger arrows
indicate
increased
activity, and
smaller arrow
indicated
decreased
activity. The
cortex provides
glutamatergic
input to the
striatum, which
also receives
dopaminergic
projections from
the substantia
nigra. In
Parkinson’s
disease, the
dopaminergic
neurons die and
there is a loss of
dopaminergic
input into the
striatum. As a
result, the
decreased D1
receptor
activation in the
caudate causes
the caudate cells
to be less active,
releasing less
GABA onto the
globus pallidus
internal segment.
The globus
pallidus internal
segment is
disinhibited, and
thus increases its
firing rate,
releasing more
GABA onto the
thalamus. The
decreased D2
receptor
activation in the
putamen causes
the putamen
cells to be more
active, releasing
more GABA onto
the globus
pallidus external
segment. The
globus pallidus
releases less
GABA onto the
subthalamic
nucleus. The
subthalamic
nucleus is
disinhibited, and
increases its
release of
glutamate onto
the GPi. This
causes the GPi to
increase its firing
rate, releasing
more GABA onto

the thalamus.
Together, the
decreased
activity of the
thalamus
decreases activity
of the motor
cortex and thus
decreases
movement.
‘Model of Basal
ganglia circuitry
in Parkinson’s
disease- indirect
pathway’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Diagnosis and Treatment

Parkinson’s disease is considered idiopathic because there is not a single known cause. Unfortunately,
there are not measurable biomarkers (blood tests, brain scans) to diagnose Parkinson’s disease. Rather,
diagnosis of individuals is typically done by a neurologist and a detailed exam. This can be further
confirmed by giving medications that are used to treat Parkinson’s disease and determining if
symptoms decrease.
A diagnosis of Parkinson’s disease is confirmed post-mortem through:
1. The loss of dopaminergic substantia nigra cells

2. Presence of Lewy bodies
Lewy bodies are intracellular alpha-nuclein protein aggregates that are believed to potentially displace
healthy neurons and lead to the neurodegeneration observed in the disease.
Figure 46.7. Lewy

bodies. This
microscope
image is showing
a Lewy body
(denoted by the
black triangle) in
the substantia
nigra of an
individual with
Parkinson’s
disease.
Dopamine cannot be administered as a treatment to those with Parkinson’s disease to replace the
loss of dopamine from the substantia nigra due to the inability of dopamine to cross the blood-
brain-barrier. Instead, the major drug treatment for the motor symptoms of Parkinson’s disease has
been administration of the drug levodopa (L-DOPA). L-DOPA is an intermediate in the synthesis
of dopamine. Typically, it is converted into dopamine through the activity of DOPA decarboxylase.
It is typically co-administered with other drugs to help prolong its effects. Unfortunately, there are
many side effects of L-DOPA including nausea, joint stiffness, dyskinesias (involuntary movements
and tics), and psychosis.
Figure 46.8.
Dopamine
synthesis
pathway.
Tyrosine is
converted into
L-DOPA through
tyrosine
hydroxylase.
L-DOPA is then
converted into
dopamine by the
enzyme DOPA
decarboxylase.
‘Dopamine
Synthesis’
by Casey
Henley is
licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
In a medical intervention called deep brain stimulation (DBS), a surgeon implants permanently
indwelling electrodes directly into brain tissue. These electrodes are controlled by an external battery
pack that delivers preprogrammed stimulation protocols. DBS in the STN is used to alleviate the
symptoms of Parkinson’s disease.
Figure 46.9. Deep

brain stimulation.
A deep brain
stimulator device
must be
implanted
surgically.
Electrodes are
positioned in
specific brain
areas for the
delivery of
electrical
impulses. The
impulses come
from an external
battery pack that
is typically
implanted
beneath the skin
on the chest.
Deep brain
stimulation can
be used to treat
disorders such as
Parkinson’s
disease,
obsessive
compulsive
disorder, and
depression.
Key Takeaways
• Parkinson’s disease is a neurodegenerative disease that causes dopaminergic

substantia nigra cells to die, causing dysfunction in the basal ganglia circuitry
• L-DOPA and deep brain stimulation are treatments for Parkinson’s disease
Attributions
Media Attributions
• Parkinson’s disease Symptoms © Herter, Christian Archibald adapted by Valerie Hedges is

• Parkinson’s disease substantia nigra © Bruce Blaus adapted by Valerie Hedges is licensed under a
CC BY (Attribution) license
• Parkinson’s diseases effect on D1 receptor function © Valerie Hedges is licensed under a CC BY-
• Parkinson’s disease direct pathway Basal Ganglia model © Valerie Hedges is licensed under a CC
• D2 Receptor Effects
• Parkinson’s disease indirect pathway basal ganglia model © Valerie Hedges is licensed under a
• Lewy Body © Tulemo adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• dopamine synthesis © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-
• Deep brain stimulation © NIMH adapted by Valerie Hedges is licensed under a Public Domain
license
BEHAVIOR | 681
PART VII
BEHAVIOR
682 | BEHAVIOR
NEURODEGENERATIVE DISEASE- NON MOTOR | 683
47.
NEURODEGENERATIVE DISEASE- NON

MOTOR
As we age, the human brain is subject to degradation over

our lifetime. This normal aging results in moderate cell
loss and a gradual loss of myelination and synapses. These
structural changes result in normal age-related functional Resources
losses (slower processing time or forgetting where you put
your keys).
• Scientist Links to learn

Alzheimer’s Disease more!
• Glossary terms
Alzheimer’s disease was first described In 1907 by Dr. • Key takeaways
Alois Alzheimer. He had described one of his patients, a
50-year old woman named Auguste Deter, whose
symptoms included profound cognitive impairment,
memory deficits, and delusions. After Deter’s death, post-mortem analysis of her brain revealed
anomalies: degenerating neurons that contained atypical tangles and deposits scattered between cells.
Alzheimer’s disease (AD) is not a natural result of the aging process, but there is an increased risk
of AD as we age. It is an irreversible, slowly progressing neurodegenerative condition that leads to
deficits in thinking, behavior, and memory loss. AD is a devastating disease that accounts for 60-80%
of all cases of dementia and is the sixth leading cause of death in the United States. As for prevalence,
approximately 10% of people older than 65 and nearly a third older of people older than 85 has AD.
684 | NEURODEGENERATIVE DISEASE- NON MOTOR
Figure 47.1. PET scan comparison between a normal brain and a brain of an individual with
Alzheimer’s disease. The PET scan on the left shows brain activity in a normal older adult. The
PET scan on the right shows brain activity of an older adult with Alzheimer’s disease. The
brain from the individual with Alzheimer’s disease has far less activity than the normal brain.
Specifically, declarative memory is the first form of memory loss observed in AD patients. As the
disease progresses, procedural memory loss becomes more apparent. In addition to memory loss, AD
symptoms include problems with language, disorientation, mood swings, loss of motivation, lack of
self care, behavioral problems, loss of bodily functions, and dementia.
AD is divided into two categories, familial and sporadic. Familial AD is diagnosed when a person
is in their 50s or 60s, and is strongly influenced by genetic risk factors. This form of AD only makes
up about 10% of cases. Sporadic AD is by far more common than familial AD, and is believed to
be caused by a combination of old age and environmental factors in addition to genetic risk factors.
Several genetic risk factors are linked to AD risk. Apolipoprotein epsilon4 (ApoE4) is the greatest
genetic risk factor identified, where individuals who are homozygous for the e4 polymorphism have a
12-times higher risk of developing AD than people with the more common e3 variant. Additionally,
mutations in genes like amyloid-precursor protein (APP), presenilin-1 (PSEN1) presenilin-2 (PSEN2),
and triggering receptors expressed on myeloid cells 2 (TREM2) are all associated with higher risk of
developing AD.
Brain Areas Affected in Alzheimer’s disease

In AD, brain atrophy is observed across the brain, especially in later progression of the disease, however
two structures that see the most atrophy are the hippocampus and cortex. One of the main structures
affected in Alzheimer’s disease is the hippocampus, which is located curled up inside the temporal
lobes. The hippocampus is a critical structure in learning and memory, especially in spatial memory
and in consolidation of short-term memory into long-term memory.
The prefrontal cortex is also heavily affected in AD. The prefrontal cortex is the most anterior
portion of the frontal lobe and functions in intellect, cognition, recall, problem solving, planning, and
personality.
Figure 47.2. Brain

structure
changes in
Alzheimer’s
disease. The
image on the left
shows a coronal
section of a
normal brain,
with the cerebral
cortex,
hippocampus
and entorhinal
cortex labeled.
The image on the
right shows the
brain of an
individual with
Alzheimer’s
disease. The
cerebral cortex
has significant
cell loss, which
results in
shrinkage of the
cerebral cortex.
The
hippocampus is
also heavily
affected in the
disease, and
similarly displays
shrinkage. In
Alzheimer’s
disease,
however, the
ventricles are
enlarged. This
occurs as a result
of the significant
cell loss across
the brain, and
more of the brain
being taken up
by space
(ventricles) than
in the normal
brain.
Hypotheses of Alzheimer’s Disease

Pathologically, AD is characterized by the extracellular accumulation of amyloidbeta plaques (Aβ)
that build up in the space between neurons and intracellular hyperphosphorylated neurofibrillary
tau tangles (NFT).
Beta Amyloid Hypothesis
The amyloid cascade hypothesis, originally proposed in 1992, has become the leading theory in the
field describing how AD develops. The hypothesis suggests that the main driving factor of AD is the
deposition of Aβ in the brain, and this in turn leads to neurodegeneration via cell death, abnormal
protein buildup, and neuroinflammation.
Aβ is produced from the cleavage of amyloid-precursor protein (APP), an integral membrane
protein normally expressed by neurons that typically functions as a cell surface receptor and is
important in neuronal adhesion, neurite growth, and axogenesis. In AD, the extracellular soluble
portion of APP is cleaved by the enzyme beta-secretase and then the enzyme gamma secretase cleaves
the APP anchor, leaving Aβ protein that is no longer held within the membrane. Aβ is likely to clump
together in unpredictable ways within the extracellular space, leading to the formation of Aβ plaques
(pronounced “A beta”). These plaques can cause neuronal death and lead to the cognitive deficits
observed in AD patients.
Figure 47.3.
Amyloid
precursor protein
processing. The
extracellular
portion of the
amyloid
precursor protein
(APP) is cleaved
by β secretase. γ
secretase then
cleaves the
intracellular
anchor portion of
APP, leaving
amyloidbeta
protein (Aβ). The
Aβ proteins are
no longer
anchored to the
membrane, and
then are likely to
aggregate in
extracellular
spaces.
Two major genetic risk factors for AD, presenilin 1 and 2, are mutations of the gamma secretase that
leads to increased APP cleavage. Therapies are being developed to target and control the Aβ protein
to mitigate AD symptoms. Recently an antibody-based drug has been approved by the FDA. This
groundbreaking drug is unique from those traditionally prescribed because it directly targets Aβ.
However, the amyloid-cascade hypothesis does not tell the whole story. For one, there are patients who
carry a heavy Aβ load but present no clinical symptoms of AD. More so, in mouse models with APP
mutations, they develop significant Aβ plaques, but have no accumulation of tau (see below) and no
significant neurodegeneration. Additionally, there have been several drugs targeting Aβ that have failed
in human trials.
Tau Tangle Hypothesis
While these amyloid plaques seem to be the primary driving factor of AD, hyperphosphorylated tau
and neurofibrillary tangles (NFTs) are also potential culprits in disease progression. NFTs are an
intracellular pathological marker of AD and correlate strongly with cognitive deficits in AD. Tau
protein is the major microtubule-associate protein (MAP) of mature neurons and helps to function to
maintain cellular morphology. Excess phosphorylation of this protein causes tau to accumulate inside
the cell, leading to neuronal dysfunction and cell death. The presence of Aβ increases the levels of
the tau, and vice versa, adding to the complexity and difficulty of treating AD. Tau pathology is also
observed in other neurodegenerative diseases such as frontotemporal lobe dementia and Parkinson’s
disease.
Figure 47.4. Tau

protein
destabilization in
Alzheimer’s
disease. Typically
Tau is a protein
that is critical in
stabilization of
cell structure
through
interaction with
microtubules
within the cell. In
Alzheimer’s
disease, Tau
proteins are
destabilized,
causing the
structure within
neurons to break
down. The Tau
protein them
aggregate
together to form
Tau Tangles.
Although most discussion of AD revolves around plaques and tangles, it is well known that a
cacophony of pathological markers are also seen in AD, including neuroinflammation, oxidative stress,
blood-brain barrier dysfunction, heavy metal dysregulation, mitochondrial impairment, and many
more.
Acetylcholine Hypothesis
An early hypothesis proposed to explain the neuronal loss and memory deficits observed in AD is
centered around changes in the signaling of acetylcholine. Early post-mortem analyses discovered that
people with AD have profound atrophy of the basal forebrain, an area which contains a large density
of cholinergic neurons. Further, patients with Alzheimer’s disease also show decreased levels of choline
acetyltransferase, reduced choline uptake in cells, and reduced release of acetylcholine. The cholinergic
hypothesis suggests that it is this loss of cholinergic neurons and the loss of acetylcholine signaling that
is the main pathological driver of AD. The theory is supported by the idea that acetylcholine plays an
important role in learning and memory.
Figure 47.5.
Acetylcholine
synthesis and
synaptic activity.
Acetylcholine is
synthesized in
the axon terminal
through the
actions of choline
acetyltransferase
on choline and
acetyl CoA.
Acetylcholine is
packaged into
synaptic vesicles
by vesicular
acetylcholine
transporter
(VAChT). When
acetylcholine is
released into the
synapse, it binds
to postsynaptic
membrane
receptors and is
degraded by
acetylcholinester
ase within the
synapse. Early
evidence in
Alzheimer’s
disease indicated
decreased
activity of the
neurotransmitter
acetylcholine.
Evidence includes
death of
cholinergic
neurons in the
forebrain,
decreased levels
of choline
acetyltransferase,
decreased uptake
of choline into
presynaptic cells,
and reduced
release of
acetylcholine in
the synapse.
Diagnosis of Alzheimer’s Disease

To this day, only post-mortem analysis can conclusively diagnose someone with AD. As expected, this
raises many issues in terms of treating these patients. Currently, physicians diagnose a patient with
AD based on the symptoms they presented with. However, new techniques to make more accurate
diagnoses are emerging, including identifying the presence of biomarkers from blood samples, certain
functional characteristics as observed in PET or fMRI imaging, and possibly even through eye exams.
Mild cognitive disorder/impairment represents a modest level of cognitive decline that interferes
with the ability to complete everyday activities. Whereas major cognitive disorder/impairment is
considered a major impairment and includes different dementias and Alzheimer’s disease.
Figure 47.6. Comparison of clock drawing exercise between a normal individual, a patient with mild
cognitive impairment / early Alzheimer’s disease, and late Alzheimer’s disease. A normal older adult
was able to accurately draw a clock and correctly place the hands on the clock to depict the
instructed time of 2:30. In the middle image, the patient with mild cognitive impairment / early
Alzheimer’s disease can mostly recreate the clock with the numbers in the correct order. However,
the patient was not able to remember the time (2:30) to draw on the clock. In the right image, the
patient with late Alzheimer’s disease is unable to draw an accurate representation of a clock and
completely omits the clock hands that depict time.
Treatments
Most of the FDA approved drugs for AD are acetylcholinesterase (AChE) inhibitors, drugs that act to
increase levels of acetylcholine. The other approved FDA drug is an NMDA receptor antagonist. This
drug is prescribed because it is hypothesized that AD may involve glutamate excitotoxicity, where excess
binding of glutamate can increase intracellular Ca2+ levels leading to Ca2+ poisoning and neuron
death.
Unfortunately, these compounds show only short-term benefits for cognitive function, and these
therapeutic effects subside over time. The different drugs are prescribed during different stages of AD,
have modest effects and have unwanted side effects.
Key Takeaways
• Alzheimer’s disease is not a normal result of again, but is a neurodegenerative

disease that is more likely to affect older adults.
• The cortex and hippocampus are both heavily affected in Alzheimer’s disease,
resulting in brain atrophy.
• There are three hypotheses for the cause of Alzheimer’s disease
◦ The beta amyloid hypothesis involves beta amyloid plaques that form in the
extracellular spaces around neurons, ultimately causing cell death.
◦ The neurofibrillary tau tangle hypothesis involves destabilization of the tau
protein inside of neurons, resulting in the collapse of cellular structure and
aggregation of tau proteins into tangles within the cell that cause neuron
death.
◦ The acetylcholine hypothesis stems from observations that acetylcholine
signaling is decreased in Alzheimer’s disease
• Absolute diagnosis of Alzheimer’s disease occurs post-mortem. Physician

examinations can make a probable diagnosis prior to death.
• Treatments for Alzheimer’s disease include acetylcholinesterase inhibitors and NMDA
receptors antagonists.
Attributions
Media Attributions
• PET_scan-normal_brain-alzheimers_disease_brain © Health and Human Services Department,

National Institutes of Health, National Institute on Aging adapted by Valerie Hedges is licensed
under a Public Domain license
• Structural differences © Garrondo adapted by Valerie Hedges is licensed under a Public Domain
license
• APP Processing © Ipeltan adapted by Valerie Hedges is licensed under a Public Domain license
• Tau Tangles © ADEAR: "Alzheimer's Disease Education and Referral Center, a service of the
National Institute on Aging." adapted by Valerie Hedges is licensed under a Public Domain
license
• Acetylcholine signaling © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-
• Superior-pattern-processing-is-the-essence-of-the-evolved-human-brain-fnins-08-00265-g0004
© Mattson M adapted by Valerie Hedges is licensed under a CC BY (Attribution) license
MOTIVATED BEHAVIOR: FEEDING BEHAVIOR | 695
48.
MOTIVATED BEHAVIOR: FEEDING

BEHAVIOR
Motivated behaviors are voluntary behaviors that

individuals find rewarding or pleasurable. Motivation
varies in intensity which will correlate to the probability
of performing the associated behavior. Certain behaviors Resources
or stimuli, like food or sex, are naturally rewarding because
they are necessary for the survival of a species; they are
adaptive, and the nervous system has evolved to make
these behaviors pleasurable. Rewarding stimuli increases • Scientist links to learn
brain activation in brain regions that comprise the reward more!
circuit. • Glossary Terms
• Key Takeaways
Homeostasis
Homeostasis is maintenance of the body’s internal environment within a physiological range. The
hypothalamus is important in regulating many homeostatic processes by responding to sensory input
when a deviation from homeostatic set point is detected.
Role of Hypothalamus in Homeostasis and

Motivated Behavior
The hypothalamus is located ventrally on the brain and surrounds the third ventricle. Sensory
neurons within the hypothalamus measure changes in parameters that need to be regulated within the
body, such as temperature, water balance, and feeding. When that parameter is sensed to be outside
696 | MOTIVATED BEHAVIOR: FEEDING BEHAVIOR
the normal physiological range, these hypothalamic neurons will initiate a three component process
that will act to bring that parameter back within range (restore homeostasis): Humoral response,
visceromotor response, and somatic motor response.
Figure 48.1. The

hypothalamus,
shown in orange
in this coronal
view, is located
adjacent to the
third ventricle.
‘Hypothalamus
Coronal View’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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License.
Humoral Response
In the humoral response, neurosecretory cells within the hypothalamus regulate release of pituitary
hormones into circulation. The word ‘humoral’ comes from the word ‘humor’ which is an older word
that refers to different fluids within the body. In the case of the humoral response, the bodily fluid is in
reference to the blood where the hormones are being released.
Hypothalamic Control of the Pituitary Gland
The hypothalamus contains two types of neurons that secrete hormones into the pituitary:
parvocellular neurosecretory cells and magnocellular neurosecretory cells. Magnocellular
neurosecretory cells, the larger type of neurosecretory cell compared to parvocellular neurons, send
axons from the hypothalamus down to the pituitary stalk where they terminate on capillaries of the
general circulation located within the posterior pituitary. Therefore, the hormones that are released by
the posterior pituitary gland are actually made within the hypothalamus. For this reason, the posterior
pituitary is considered neural tissue, rather than endocrine tissue.
Figure 48.2. The

hypothalamic
magnocellular
neurosecretory
neurons release
oxytocin and/or
vasopressin into
the general
circulation via
capillaries located
in the posterior
pituitary.
‘Oxytocin and
Vasopressin
Release’ by Casey
Henley is
licensed under a
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Commons
Attribution
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The magnocellular neurons synthesize and release oxytocin and vasopressin, two neuropeptides, into
the blood. Oxytocin, often referred to as the love hormone, promotes social bonding. It is released
during reproduction and also causes uterine contractions during labor and the milk letdown reflex
after birth. Vasopressin, also called antidiuretic hormone, plays a role in regulating salt concentration
in the blood by acting on the kidneys to promote water retention and decrease urine production.
Vasopressin has also been shown to be involved in bonding, parenting, territoriality, and mate
guarding in some animals.
Parvocellular cells are smaller than the magnocellular neurons (parvus means “small” in
Latin). These neurons release hypophysiotropic hormones into a specialized capillary system that
lies between the hypothalamus and the anterior pituitary gland called the hypophyseal portal
circulation. The hypophysiotropic hormones cause anterior pituitary cells to make and release their
own hormones. The anterior pituitary gland is therefore endocrine tissue because the cells make their
own hormones. There are multiple hormones that are made and released by the anterior pituitary
gland. They act on multiple target tissues throughout the body, including the gonads, thyroid glands,
adrenal glands, and mammary glands.
Figure 48.3. The

hypothalamic
parvocellular
neurosecretory
neurons release
hypophysiotropic
hormones into
the hypophyseal
portal circulation,
causing the
hormone-releasi
ng endocrine
cells in the
anterior pituitary
to release
hormones. ‘CRH
and ACTH
Release’ by Casey
Henley is
licensed under
a Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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License.
Visceromotor Response
The visceromotor response involves hypothalamic neurons that adjust sympathetic and
parasympathetic activity to regulate internal organs.
Somatic Motor Response

The somatic motor response involves hypothalamic neurons that trigger an appropriate behavioral
response.
Using a drop in temperature as an example, the humoral response will cause the release of hormones
into circulation that will allow you to metabolize energy in a different way that will allow for more
generation of heat. The visceromotor response would increase sympathetic nervous system activity to
ensure that internal organs stay warm. Lastly, the somatic motor response would cause a behavioral
change that would result in getting warmer (e.g. getting a coat, moving closer to the fire, or getting out
of the cold outside).
Homeostatic Regulation of Body Weight and

Food Intake
We obtain energy from our diet and consumption of glucose, which serves as our energy source within
the body. The body depends on access to glucose for normal function and survival. To ensure access
to glucose when needed, our body stores glucose within the body in fat stores. We are motivated to eat
and maintain these body fat stores.
Energy Balance
Body fat stores are regulated after we eat a meal. When we consume more glucose than we can use, the
excess glucose is stored within the body as fat stores. Over time, this repeated pattern leads to increased
adiposity and can lead to obesity. If energy demands exceed the consumption of glucose, then glucose
must be broken down from fat stores and fat is lost. Over time, this leads to decreased adiposity and
starvation.
Figure 48.4.
Energy Balance.
Glucose is used
as energy within
the body. If
glucose intake is
equal to energy
expenditure,
then a normal
body weight will
result. When
dietary intake of
glucose is less
than the amount
of energy
expended, then
glucose stores
will be broken
down and there
will be a loss of
fat and a loss of
body weight.
When dietary
intake of glucose
is more than the
amount of
energy
expended, then
excess glucose
will be stored in
the body as fat,
increasing body
weight. ‘Energy
Balance’ by
Valerie Hedges is
licensed under a
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The body tries to defend fat stores and body weight around a particular set point. When rodents have
restricted access to calories they experience a decrease in body weight, however when food is freely
available again, they gain weight back to their weight prior to calorie restriction. Conversely, animals
that are force fed to consume more calories than normal will gain weight, but will lose that gained
weight when they are free to regulate their own feeding.
To maintain a balance of fat stores and expended energy, we regulate our feeding behavior. There are
both short-term and long-term mechanisms that contribute to feeding regulation and maintenance of
fat stores.
Long-term Regulation of Feeding Behavior
Leptin
The level of fat within the body must somehow be communicated to the brain in a way that ultimately
controls feeding behavior. The discovery of genetically obese mice by Dr. Douglas Coleman and
colleagues helped determine the source of this signal. The DNA of the genetically obese mice found
that these animals were missing both copies of the ob gene. These mice are referred to as ob / ob mice.
Figure 48.6. Normal mouse vs ob / ob mouse. This pictures shows an ob / ob mouse on the left and
a normal mouse on the right. The ob / ob mouse has significantly more fat store than the normal
mouse.
It was theorized that the ob gene must produce a hormone that typically signals that fat reserves are
sufficient. Without this gene, and thus without this hormone, the obese mice were not receiving the
appropriate signal to stop eating, causing them to over eat and increase fat stores. It was determined
that the ob gene codes for the hormone leptin, which is secreted by fat cells. The ob mice had sufficient
levels of fat, but did not have the hormone leptin released by the fat cells that would typically decrease
feeding behavior. In fact, when ob mice are treated with leptin, they no longer over eat and restore a
normal body weight.
More leptin is secreted when fat levels are high. Leptin acts to decrease appetite and increase energy
expenditure, which will ultimately decrease levels of fat, and maintain normal fat stores levels in the
body. Though it may seem that leptin administration would be an effective treatment for obesity,
leptin treatment is only effective for individuals with an identified leptin deficiency. Those without a
leptin deficiency have increased levels of leptin due to increased fat stores, but ultimately have neurons
with decreased sensitivity to leptin levels.
Role of Hypothalamus in Feeding Behavior

Two areas of the hypothalamus have been historically described with regard to their role in feeding
behavior through lesion studies. If the lateral hypothalamus is bilaterally lesioned, it results in
lateral hypothalamic syndrome and anorexia, whereas bilateral lesions of the ventromedial
hypothalamus results in ventromedial hypothalamic syndrome and overeating / obesity.
Though it follows that the lateral hypothalamus causes feeding behavior to increase and the
ventromedial hypothalamus causes feeding behavior to decrease, leptin signaling within these
structures underlies these behavioral changes.
Figure 48.5.
Hypothalamic
Nuclei. A coronal
section of the
brain showing
the location of
the
hypothalamus.
The
hypothalamus is
then enlarged to
show the
location of
multiple
hypothalamic
nuclei. There are
multiple nuclei
within the
hypothalamus
that regulate
feeding behavior.
The arcuate
nucleus (shown
in blue) is
adjacent to the
third ventricle.
Dorsal to the
arcuate nucleus
is the
ventromedial
nucleus (shown
in purple). The
paraventricular
nucleus sits
adjacent to the
dorsal area of the
third ventricle
(shown in
orange). The
lateral
hypothalamus is
not a nucleus,
but has cells that
all perform the
same function
(shown in green).
‘Hypothalamic
Nuclei’ by Valerie
Hedges is
licensed under a
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Commons
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Arcuate Nucleus
The neurons within the arcuate nucleus of the hypothalamus have receptors that detect levels of
circulating leptin. The arcuate nucleus neurons produce peptide neurotransmitters called αMSH
(alpha-melanocyte-stimulating hormone) and CART (cocaine- and amphetamine-regulated
transcript). Note, that the names of peptides are typically derived from the first identified function
of the peptide, but that additional functions are later discovered, which can lead to the names of the
peptides being somewhat confusing.
Figure 48.7.
αMSH and CART
arcuate neuron
projections. A
population of
αMSH and CART-
producing
arcuate neurons
(blue colored
neurons) are
activated by high
levels of leptin.
These neurons
project to three
different
locations to bring
about the
humoral,
visceromotor,
and somatic
motor responses
of the
hypothalamus.
Projections from
the arcuate to
the
paraventricular
nucleus (PVN)
controls the
humoral
response.
Projections from
the arcuate to
the brainstem
(BS) controls the
visceromotor
response. Lastly,
projections from
the arcuate to
the lateral
hypothalamus
(LH) controls the
somatic motor
response. ‘αMSH
and CART
arcuate neuron
projections’ by
Valerie Hedges is
licensed under a
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ommercial-Share
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Humoral Response to Increased Leptin

Changes in leptin levels will lead to the humoral, visceromotor, and somatic motor hypothalamic
responses.
When fat levels are increased, leptin levels are also increased. In the humoral hypothalamic response,
leptin binds to neurons in the arcuate nucleus. This population of arcuate neurons project to the
paraventricular nucleus (PVN) and release αMSH and CART onto the PVN neurons. In response
to the binding of αMSH and CART, the PVN neurons cause the release of the hypophysiotropic
hormones. The anterior pituitary gland will then release thyroid stimulating hormone (TSH) and
adrenocorticotropic hormone (ACTH), which will both act to raise the metabolic rate within the
body.
Figure 48.8. High

leptin humoral
response. When
leptin levels are
high neurons
within the
arcuate nucleus
release αMSH
and CART onto
neurons within
the
paraventricular
nucleus (PVN),
controlling the
humoral
response of the
hypothalamus. In
response to
αMSH and CART,
the PVN neurons
cause the release
of the
hypophysiotropic
hormones. The
anterior pituitary
gland then
releases thyroid
stimulating
hormone (TSH)
and
adrenocorticotro
pic hormone
(ACTH), which
will both act to
raise the
metabolic rate
within the body.
‘High leptin
humoral
response’ by
Valerie Hedges is
licensed under a
Creative
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ommercial-Share
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Visceromotor Response to Increased Leptin

When leptin levels are high, leptin will also bind to a separate population of arcuate neurons that
project to the brainstem and control the hypothalamic visceromotor response. These arcuate neurons
release αMSH and CART onto brainstem neurons that will act to increase sympathetic nervous
system activity, raising the metabolic rate and increasing body temperature.
Figure 48.9. High

leptin
visceromotor
response. When
leptin levels are
high neurons
within the
arcuate nucleus
release αMSH
and CART onto
neurons within
the brainstem
(BS), controlling
sympathetic
nervous system
activity and the
visceromotor
response of the
hypothalamus.
αMSH and CART
cause increased
sympathetic
nervous system
activity, which
will increase
body
temperature and
increase
metabolic rate
within the body.
‘High leptin
visceromotor
response’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
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Somatic Motor Response to Increased Leptin

Lastly, when leptin levels are high, leptin binds to a third population of arcuate neurons that project
to the lateral hypothalamus and control the hypothalamic somatic motor response. These arcuate
neurons release αMSH and CART onto lateral hypothalamus neurons. αMSH and CART inhibit the
lateral hypothalamus, inhibiting feeding behavior.
Figure 48.10.
High leptin
somatic motor
response. When
leptin levels are
high neurons
within the
arcuate nucleus
release αMSH
and CART onto
neurons within
the lateral
hypothalamus
(LH) controlling
the somatic
motor response
of the
hypothalamic.
αMSH and CART
inhibit the
neurons within
the lateral
hypothalamus
that normally
promote feeding
behavior, thereby
causing
decreased
feeding. ‘High
leptin somatic
motor response’
by Valerie
Hedges is
licensed under a
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Commons
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ommercial-Share
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Overall, the increase in metabolic rate, increase in body temperature, and decreased feeding will all
ultimately lead to a decrease in body fat and thus a decrease in leptin levels, bringing fat and leptin
levels back within the physiological setpoint range. αMSH, CART, and Leptin are all examples of
anorexigenic, or satiety, signals. Collectively, anorexigenic signals decrease feeding behavior and
decrease body weight in response to increased adiposity.
Humoral Response to Decreased Leptin

When fat levels are decreased, leptin levels are also decreased. When leptin levels drop, an entirely
different set of neurons within the arcuate nucleus are activated that release the neuropeptides
neuropeptide Y (NPY) and agouti-related peptide (AgRP).
Figure 48.11.
AgRP and NPY
arcuate neuron
projections. A
population of
AgRP and NPY-
producing
arcuate neurons
(pink colored
neurons) are
activated by low
levels of leptin.
These neurons
project to three
different
locations to bring
about the
humoral,
visceromotor,
and somatic
motor responses
of the
hypothalamus.
Projections from
the arcuate to
the
paraventricular
nucleus (PVN)
controls the
humoral
response.
Projections from
the arcuate to
the brainstem
(BS) controls the
visceromotor
response. Lastly,
projections from
the arcuate to
the lateral
hypothalamus
(LH) controls the
somatic motor
response. ‘AgRP
and NPY arcuate
neuron
projections’ by
Valerie Hedges is
licensed under a
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Commons
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ommercial-Share
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In the humoral hypothalamic response, the drop in leptin activates neurons in the arcuate nucleus.
This population of arcuate neurons project to the paraventricular nucleus (PVN) and release NPY
and AgRP onto the PVN neurons. These neuropeptides inhibit the PVN, thereby inhibiting release
of the hypophysiotropic hormones, and ultimately inhibiting release of TSH and ACTH. As a result,
metabolic activity in the body is decreased.
Figure 48.12. Low

leptin humoral
response. When
leptin levels are
low, neurons
within the
arcuate nucleus
release AgRP
and NPY onto
neurons within
the
paraventricular
nucleus (PVN),
controlling the
humoral
response of the
hypothalamus. In
response to
AgRP and NPY,
the PVN neurons
are inhibited, and
thus do not
release
hypophysiotropic
hormones. As a
result, -the
anterior pituitary
gland does not
release thyroid
stimulating
hormone (TSH)
and
adrenocorticotro
pic hormone
(ACTH), causing
metabolic rate
within the body
to decrease. ‘Low
leptin humoral
response’ by
Valerie Hedges is
licensed under a
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Visceromotor Response to Decreased Leptin

When leptin levels are low, arcuate neurons are activated that project to the brainstem and control the
hypothalamic visceromotor response. These arcuate neurons release NPY and AgRP onto brainstem
neurons that will act to increase parasympathetic nervous system activity, decreasing the metabolic rate
and decreasing body temperature.
Figure 48.13. Low

leptin
visceromotor
response. When
leptin levels are
low, neurons
within the
arcuate nucleus
release AgRP
and NPY onto
neurons within
the brainstem
(BS), controlling
parasympathetic
nervous system
activity and the
visceromotor
response of the
hypothalamus.
AgRP and NPY
cause increased
parasympathetic
nervous system
activity, which
will decrease
body
temperature and
decrease
metabolic rate
within the body.
‘Low leptin
visceromotor
response’ by
Valerie Hedges is
licensed under a
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ommercial-Share
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Somatic Motor Response to Decreased Leptin

Lastly, when leptin levels are low, the drop in leptin activates a population of arcuate neurons that
project to the lateral hypothalamus and control the hypothalamic somatic motor response. These
arcuate neurons release NPY and AgRP onto lateral hypothalamus neurons. NPY and AgRP stimulate
the lateral hypothalamus, stimulating feeding behavior.
Figure 48.14.
Low leptin
somatic motor
response. When
leptin levels are
low, neurons
within the
arcuate nucleus
release AgRP
and NPY onto
neurons within
the lateral
hypothalamus
(LH) controlling
the somatic
motor response
of the
hypothalamic.
AgRP and NPY
activate the
neurons within
the lateral
hypothalamus
that promote
feeding behavior,
thereby causing
increased
feeding. ‘Low
leptin somatic
motor response’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Overall, the decrease in metabolic rate, decrease in body temperature, and increased feeding brought
about by the three hypothalamic responses will all ultimately lead to an increase in body fat and thus
an increase in leptin levels, bringing fat and leptin levels back within the physiological setpoint range.
NPY and AgRP are examples of orexigenic signals. Orexigenic signals increase feeding behavior
and increase body weight in response to changes in adiposity. AgRP and αMSH are considered
antagonistic neurotransmitters because they both bind to the MC4 receptor on hypothalamic
neurons, but exert opposite effects on feeding behavior.
Key Takeaways
• Motivated behaviors are voluntary behaviors that individuals find rewarding

• Certain behaviors or stimuli are naturally rewarding because they are
necessary for the survival of a species
• The hypothalamus has three responses to help maintain homeostasis:
humoral response, visceromotor response, somatic motor response
• Leptin levels are controlled by fat level in the body
• Leptin interacts with 2 different populations of neurons within the arcuate
nucleus of the hypothalamus to bring about homeostasis
Attributions
Media Attributions
• Hypothalamus Coronal © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• PosteriorPituitary © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Anterior Pituitary © Casey Henley adapted by Valerie Hedges is licensed under a CC BY-NC-SA
• Energy balance © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• ob/ob mouse © BigPlankton is licensed under a Public Domain license
• Hypothalamic brain areas involved in feeding © Valerie Hedges is licensed under a CC BY-NC-
• High Leptin aMSH and CART neurons © Valerie Hedges is licensed under a CC BY-NC-SA
• High Leptin Humoral © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• High Leptin Visceromotor © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• High Leptin Somatic Motor © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Low Leptin NPY and AgRP neurons © Valerie Hedges is licensed under a CC BY-NC-SA
• Low Leptin Humoral © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Low Leptin Visceromotor © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Low Leptin Somatic Motor © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
MOTIVATED BEHAVIOR: REWARD PATHWAY | 723
49.
MOTIVATED BEHAVIOR: REWARD

PATHWAY
Having a sense of reward is a valuable adaptive trait.

Consider two similar organisms living in the distant
geologic past, both involved in Darwin’s struggle to pass Resources
their genetic material to the following generation. One of
these creatures gets an internal rewarding sensation
whenever it drinks water while thirsty, eats high caloric-
• Glossary terms
content food, or successfully reproduces. As can be
expected, this organism is highly motivated to seek out
more!
those rewarding stimuli in the world. It is are more likely
• Key Takeaways
to crave sugary foods to maximize daily caloric intake
necessary for activity and growth. The other creature does
not experience pleasure from its actions, resulting in little
motivation to seek out calories or to reproduce, for example. It stands to reason that the first of these
two creatures has an evolutionary advantage to survive. As can be expected, the regions of the brain
that are concerned with reward are highly conserved through evolution.
Reward Circuit
For us humans, our reward signaling neural circuitry starts with the ventral tegmental area (VTA).
Located in the midbrain, the VTA contains neurons that synthesize the neurotransmitter dopamine
(DA). This population of DA cells is similar to the cells in the substantia nigra, the midbrain neurons
that are lost in Parkinson’s disease.
724 | MOTIVATED BEHAVIOR: REWARD PATHWAY
Figure 49.1. The

ventral
tegmental area
(orange region) is
located in the
midbrain region
near the
substantia nigra
(green region).
Both regions
release dopamine
onto
downstream
targets. ‘Ventral
Tegmental Area’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
There are two primary pathways from the VTA that are important for reward.
The mesolimbic pathway consists of dopamine producing neurons that release dopamine onto the
cells in the nucleus accumbens (NAc, sometimes also called the ventral striatum). This seems to be the
major pathway by which reward is mediated by the brain. Using microdialysis, it is possible to measure
the dopamine concentration in the NAc during performance of some behavioral tasks. In studies with
rats, engaging in rewarding activities such as eating or sex results in increased release of dopamine in
the NAc. Similarly we see that release of dopamine into the human NAc is enhanced when we engage
in activities we enjoy: binge eating sugary stuff, having sex, even playing video games. Notably, many
drugs of abuse such as cocaine or amphetamine can induce a similar overflow of dopamine release when
an animal is exposed to the drug.
The second area that receives VTA innervation is the prefrontal cortex (PFC), and this projection
is called the mesocortical pathway. We think of the PFC as being involved in the conscious, decision
making and inhibition of action.
Figure 49.2. The

ventral
tegmental area
(orange region)
releases
dopamine into
the nucleus
accumbens
(purple region)
via the
mesolimbic
pathway and
releases
dopamine into
the prefrontal
cortex (blue
region via the
mesocortical
pathway.
’Mesolimbic and
Mesocortical
Pathways’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.

One of the earliest experiments demonstrating functional evidence of a “reward circuit” was
conducted at McGill University in the early 1950’s. Drs. James Olds and Peter Milner carried out
surgery on anesthetized rats to implant metal electrodes into different areas of the brain. After recovery,
the rats were placed into an operant conditioning chamber, also called a Skinner box. These special
cages are equipped with a device which the subject can physically manipulate. For example, the cage
may have a lever which the animal can push, or a hole into which they can poke their nose.
In the case of Olds and Milner’s experiments, a lever press led to activation of the implanted
electrodes, resulting in neuronal activation. They had found that when the electrode was implanted
into brain areas such as the corpus callosum or the hippocampus, the rats did not spend significant
time pressing on the lever. However, the rats which had the electrodes implanted in the medial
forebrain bundle that is located in the VTA to NAc pathway, responded frequently on the lever. One
such rat pressed the lever almost 2000 times in less than an hour, averaging nearly one response every
two seconds! Electrical activation of reward centers is so desirable, rats will choose electrical stimulation
over food even to the point of starvation. This experimental paradigm is also called intracranial-self
stimulation (ICSS). Treatment with drugs that block the receptors for dopamine reduce the self-
stimulating behavior, indicating that dopamine is the critical neurotransmitter involved in making the
stimulation of these brain regions rewarding.
Figure 49.3.
When an activity
like bar pressing
is paired with
stimulation of
the reward
system circuitry,
rats will show a
marked increased
in the behavior
(center panel)
compared to
controls (left
panel). If a
dopamine
receptor
antagonist is
given in addition
to the bar press
stimulation, the
behavior
decreases,
presumably
because
dopamine cannot
have its reward
effect (right
panel).
‘Dopamine
Pathway
Stimulation’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
And while the original studies were conducted on rats, clinicians soon after tested their model in
humans, with similar indwelling electrodes in different brain regions. Unsurprisingly, humans also
have areas of the brain that are responsible for encoding reward, and when given the chance to
electrically activate those areas, they do so extensively. When the experimenters brought in a tray of
delicious food to the test chamber, the hungry patients – who hadn’t eaten for upwards of seven hours
– simply looked at the meal, but couldn’t pull themselves away from the stimulation button long
enough to eat. Artificial stimulation of these reward centers of the brain was more valuable to the
patients than nutrition.
However, continued research suggests the connection between dopamine release and reward may
not be as simple as the self-stimulation studies imply. It appears that it is not the reward itself that
increases dopamine, but the predicted expectation of the reward . Dopamine signaling increases during
anticipation of a predicted reward. If the level of reward is more than predicted, reward learning occurs,
and dopamine signaling and motivation to repeat that behavior increases. If the level of reward is less
than predicted, then dopamine signaling decreases as does motivation to repeat the behavior.
Rewarding stimuli
Natural rewards that increase survival and fitness of a species activate the reward circuit. These
behaviors and stimuli include certain food (like those containing high sugar or fat levels), social
bonding, parental bonding, and sex. Most drugs of abuse also activate the reward circuit and dopamine
signaling, which plays a critical role in the formation of addiction. For example, cocaine blocks
dopamine reuptake into presynaptic VTA terminals; heroin and nicotine increase dopamine release
from the VTA. These alterations increase dopamine effect on neurons in the nucleus accumbens.
Figure 49.4.
Control (top)
panel: Dopamine
effects are
typically
terminated by
reuptake into the
presynaptic
terminal via the
dopamine
transporter
(DAT). Cocaine
treatment
(bottom) panel:
Cocaine blocks
DAT, preventing
reuptake of
dopamine. The
increased action
of dopamine on
the nucleus
accumbens leads
to increased
activation of the
reward circuit, a
mechanism
underlying
addiction to the
drug. ‘Cocaine
Effects’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Key Takeaways
• The reward circuit involves dopamine release from the ventral tegmental area into
the nucleus accumbens and prefrontal cortex.
• Self-stimulation experiments demonstrate the role of dopamine and the reward
circuit
• Dopamine signaling likely predicts reward value and can be altered if predicted
outcomes differ from actual outcomes
• Drugs of abuse act upon the reward circuit
Attributions
Media Attributions
• Ventral Tegmental Area © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• Mesolimbic Mesocortical Pathways © Casey Henley is licensed under a CC BY-NC-SA
• DopaminePathwayStimulation © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• Cocaine Effect © Casey Henley is licensed under a CC BY-NC-SA (Attribution
732 | MOTIVATED BEHAVIOR: SEXUAL BEHAVIOR
50.
MOTIVATED BEHAVIOR: SEXUAL

BEHAVIOR
Here we will examine an additional rewarding behavior.

Rodents, such as rats, are used as a model for studying
sexual behavior and reward.
Resources
Rodent Sexual Behavior
• Glossary terms When in the presence of a sexually receptive female rat, a

• Key Takeaways male rat will engage in male sexual behavior, called
mounting behavior. Mounting behavior is controlled by
the medial preoptic area (mPOA) of the
hypothalamus in males.
Female rats display their own sexual behavior called lordosis. Lordosis behavior is displayed as an
arched-back posture that is controlled by the ventromedial hypothalamus (VMH). Female rats also
have the ability to pace their sexual interactions by controlling the timing of the sexual interaction. The
female rat will run around the cage and choose when they will stop and allow the male to engage in
mounting behavior.
Steroid hormones such as testosterone, estrogen, and progesterone regulate activity of the mPOA
and VMH.
MOTIVATED BEHAVIOR: SEXUAL BEHAVIOR | 733
Figure 50.1. Rat

Sexual Behavior.
The male rate
(gray) displays
mounting
behavior and the
white rate
(female) displays
lordosis behavior,
which involves
arching of the
back. ‘Rat Sexual
Behavior’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
When considering sexual behavior as a model for motivated behaviors, it is important that we
understand why an animal engages in sexual behavior.
A modified operant chamber was constructed by Everitt and colleagues (1990) that helped to answer
this question. A male rat was trained to press a lever within the chamber that when pressed would open
a door and allow for access to a sexually-receptive female rat, with whom the male could engage in a
sexual bout. Males easily learned this paradigm and consistently pressed the lever to gain access to the
female (Everitt, 1990).
When the mPOA was lesioned in the male rats and they were tested in this same operant chamber,
the male rats still pressed the lever to gain access to the female rat, even though they no longer had the
ability to perform sexual mounting behavior. However, when the nucleus accumbens was lesioned and
the mPOA remained intact, the male rats no longer pressed the lever. They had lost the motivation
associated with the prospect of sexual experience, thus demonstrating that the nucleus accumbens, and
the reward pathway drive the motivation for sexual behavior in rodents, not the brain structure that
controls sexual behavior. It follows that in female rats, the motivation to engage in sexual behavior is
dependent on activity of the nucleus accumbens, and not on activity within the VMH.
Nucleus Accumbens Dopamine in Sexual Behavior

Measuring extracellular dopamine concentrations outside the nucleus accumbens through
microdialysis techniques has demonstrated that extracellular dopamine increases during many different
behaviors considered to be rewarding, including male and female sexual behavior.
Measuring Reward- Conditioned Place

Preference
Conditioned place preference (CPP) is a behavioral test that can be used as way of measuring the
reward associated with different stimuli. CPP is commonly used as a way to measure reward associated
with different drugs of abuse, but the paradigm can also be used to measure the rewarding aspects of
natural rewards, such as sexual behavior. In a CPP paradigm, a CPP appartus is used that typically has
two distinct testing chambers that have different colors or patterns on the walls, and different tactile
surfaces on the floor of the chamber. Additionally, there is a neutral compartment between the two
testing chambers. As an example, one testing chamber might have white walls and hard bedding on the
floor, whereas the other testing chamber has gray walls and soft bedding.
Figure 50.2. Conditioned place preference apparatus. This conditioned place preference
apparatus has a clear central compartment that serves as a neutral area. There are two
testing chambers that differ in color and tactile sensation on the floor of the chamber. The
gray chamber has soft aspen bedding and the white chamber has hard corncob bedding.
During testing, a potentially rewarding stimulus is paired with a specific environment (eg.
gray chamber) whereas the opposite environment is not paired with any substance or
behavior. ‘Conditioned place preference apparatus’ by Valerie Hedges is licensed under a
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
An experimental male animal can be placed in the neutral central compartment and then given 10
minutes to explore the entirety of the apparatus. The amount of time that the animal spends in
each chamber is recorded as a pre-test measurement. Next, the chambers are sectioned off from
each other and the experimental male animal is placed with a sexually-receptive female animal in
the gray chamber with soft bedding. The experimental male then has a 10-minute sexual experience
within the gray chamber. After the sexual experience, they are also placed alone in the white chamber
with hard bedding. This is repeated for several weeks, such that the animals learn to associate sexual
experience with the gray chamber environment and learn to associate nothing with the white chamber
environment. Lastly, the experimental animal is placed alone in the central neutral compartment and
again permitted to access the entire apparatus. The amount of time spent in each of the chambers is
recorded as a post-test measurement.
By comparing the time spending in the conditioning chamber during the pre-test to the time
spent in the conditioning chamber during the post-test, it can be determined if the animal spends
significantly more time in the chamber that was paired with the sexual experience. Researchers
interpret a significantly increased time spent in the conditioning compartment as a measurement of
the reward that the animal feels being in that environment. If no significant difference is observed
between the pre-test and post-test times in the conditioning compartment, then the testing substance
or behavior is not considered to be rewarding to the animal.
Using this paradigm, administration of a dopamine antagonist to animals prior to the conditioning
sessions prevents the formation of a conditioned place preference, indicating that dopamine within the
reward pathway underlies the reward associated with sexual behavior.
Key Takeaways
• Male rodent sexual behavior is called mounting behavior, and it is controlled by the
medial preoptic area of the hypothalamus.
• Female rodent sexual behavior is called lordosis behavior, and it is controlled by the
ventromedial hypothalamus
• Lesions to the areas of the brain that control the ability to perform the sexual
behavior does not impact the desire that the animal has to engage in sexual
behavior. Only lesions to the nucleus accumbens removes the desire to engage in
sexual behavior.
• Conditioned place preference is a behavioral paradigm that is used to determine
whether a substance or a behavior is rewarding.
• Dopamine within the reward circuit underlies the reward associated with sexual
behavior.
References
“Sexual Motivation: A Neural and Behavioural Analysis of the Mechanisms Underlying Appetitive
and Copulatory Responses of Male Rats” 1990. Everitt BJ. Neuroscience and Biobehavioral Reviews,
Vol 14 pp 217-232.
Media Attributions
• Rat sex behavior © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

• CPP apparatus © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
738 | DRUG ADDICTION
51.
DRUG ADDICTION
In the United States, more than 59 million people aged 12

and over have used illegal drugs or misused prescription
drugs within the last year. Use of some of these drugs carry
Resources significant health risks, and their use has led to hundreds
of thousands of deaths, making drug use one of the
highest preventable causes of mortality. For some people,
drug use turns into a substance use disorder, or addiction.
• Glossary Terms Importantly, many more individuals misuse drugs than
• Links are drug-dependent.
• Key Takeaways
What is drug addiction?

Substance use disorder, typically referred to as addiction has many different facets.
The American Society of Addiction Medicine defines drug addiction as “a primary, chronic disease
of brain reward, motivation, memory and related circuitry. Dysfunction in these circuits leads to
characteristic biological , psychological, social and spiritual manifestations. This is reflected in an
individual pathologically pursuing reward and/or relief by substance use and other behaviors.”
Diagnosis of Substance Use Disorder

One of the major challenges with understanding substance use disorder and other brain disorders is
related to the difficulty of making an accurate diagnosis – as almost everything in biology exists on
a spectrum. To help establish a diagnosis, the American Psychiatric Association (APA) has put
together a series of criteria for psychiatrists to diagnose these complex conditions. The guidelines are
compiled in a book called the Diagnostic and Statistical Manual of Mental Disorders. They are
DRUG ADDICTION | 739
currently on the fifth revision of the text, referred to as the DSM-5. It’s an imperfect set of criteria, but
it is a start towards understanding these remarkably complicated conditions.
Figure 51.1.
DSM-5. The
Diagnostic and
Statistical
Manual of
Mental Disorders
is a guide book
that is used for
the diagnosis of
substance abuse
disorder as well
as multiple other
mental disorders.
According to DSM-5, a substance use disorder may be an appropriate diagnosis when at least two of
the following characteristics occur within a 12-month period of time and cause significant impairment
or distress:
• The quantity of the substance used or the amount of time spent using is often greater than
intended;
• Efforts to control use of the substance are unsuccessful due to a persistent desire for the
substance;
• Considerable time is spent using the substance, recovering from its effects, or attempting to
obtain the substance;
• A strong desire, craving, or urge to use the substance is present;
• Substance use interferes with major role obligations at work, school, or home;
• Use of the substance continues despite harmful social or interpersonal effects caused or made
worse by substance use
• Participation in social, work, or leisure activities is avoided or reduced due to substance use;
• Substance use occurs in situations where substance use may be physically hazardous;
• Continued substance use occurs even when the substance is causing physical or psychological
problems or making these problems worse;
• Tolerance for the substance develops, including a need for increasing quantities of the substance
to achieve intoxication or desired effects or a noticeable decrease in effects when using the same
amount of the substance;

• After heavy or sustained use of a substance, reduction in or abstinence from the substance
results in withdrawal symptoms or precipitates resumption of use of the substance or similar
substances to relieve or avoid withdrawal symptoms.
Cost of Substance Use

Substance use disorder is prevalent worldwide. On top of the direct health risks associated with
compulsive drug use, drug addiction has a tremendous financial burden: Alcohol, tobacco, and illicit
drugs cost the US more than $740 billion dollars in lost productivity, health care costs, and legal related
fees and fines.
In the early 1900s, US laws regulating the manufacturing, distribution, possession, and use of drugs
were complicated. Certain substances were banned, but regulation of the substances was left largely to
individual states. It wasn’t until 1970 when a nationwide proposed regulatory measure would combine
all of the current federal mandates regarding misused drugs into a single bill, and Richard Nixon
signed the Controlled Substances Act (CSA) into law. Under the terms of the CSA, the Drug
Enforcement Agency and the Food and Drug Administration would be responsible for developing
regulations about a wide variety of chemical substances. For each drug, they would be tasked with
evaluating the health-related properties of the drug and its impact on health, including toxicity, harm,
and addiction potential.
Despite a dramatic increase in the cost of drug enforcement that followed the signing of the
Controlled Substances Act, the rate of drug addiction in the United States has remained unchanged.
Abused Substances
Drugs are defined as chemical substances that come from outside the body; drugs are exogenous
substances that can influence a person’s physical or mental state. Keeping in mind the impact on
health and addiction potential, drugs are classified into one of five categories, rated Schedule I through
Schedule V.
Drugs put into Schedule I are substances that were perceived to have the highest addiction
potential, most severe health risks, and no medical applications. On the other end of the spectrum
are Schedule V substances, drugs with low addiction potential, few health risks, and legitimate uses
within the clinical setting. Substances classified under Schedule I include heroin, for example, while a
Schedule V substance might be an antiepileptic drug like Lyrica. Today, the list of controlled substances
contains more than 400 chemicals. Interesting, neither tobacco or alcohol is regulated under the terms
of the Controlled Substances Act, despite these drugs being among the most misused in the US with
tremendous health risks, high addiction potential, and little medical application. There are probably
thousands and thousands of psychoactive substances, chemicals that can act on the nervous system
which can induce a change in behavior or mind state.
Some major abused drugs are described below.
Alcohol
According to the 2015 National Survey on Drug Use and Health, alcohol is the most widely used
substance among these drugs, with more than 85% of adults reporting having used alcohol at some
point in their lives. Chemically speaking, many substances are classified as “alcohols,” but the most
common misused is ethyl alcohol or ethanol. Ethanol is an easily-obtained substance with a highly
intoxicating effect. The overwhelming majority of ethanol use is recreational. The World Health
Organization reports that alcohol use is the leading risk factor for premature death among males aged
15-59 globally, making it one of the overall top causes of preventable death. Acute exposure to ethanol
has effects that differ depending on dosage.
At low concentrations, ethanol can induce an elevation in mood, decreased anxiety, increased risk
taking, slowing of reflexes, and impaired judgment. At high concentrations, ethanol can cause memory
deficits, loss of consciousness, analgesia and areflexia, and possible death through respiratory
depression. Chronic exposure to low levels of alcohol has some pro-health benefits such as decreased
risk of death by cardiovascular events, but in general, these benefits are outweighed by negative health
outcomes.
Nicotine
Nicotine is the main psychoactive ingredient of tobacco products. Nicotine is an alkaloid compound
that is synthesized in nature by a variety of plants, with the tobacco plant having the highest nicotine
content. Other plants of the Solanaceae family (Nightshade family), such as eggplants and tomatoes,
also contains nicotine, as it was probably an anti-herbivory evolutionary adaptation. The most
common routes of administration for nicotine include inhalation (cigarettes, cigars, vape),
transbuccal (chew, dip, snuff), or transdermal (in the case of the nicotine replacement therapeutic
strategy, the nicotine patch). Nicotine is a highly addictive substance, often rated as addictive as cocaine
or heroin. It is currently the leading cause of preventable death in the US, causing nearly one out of
every five deaths.
When a person smokes a cigarette, they experience a rapid rush or a high within tens of seconds.
Chronic smoking greatly increases the risk of developing lung, throat, and mouth cancers, coronary
heart disease, or stroke. Cigarette smoking over the past several decades has seen a sharp decline,
in part due to the success of anti-smoking campaigns in changing public opinion toward smoking,
and government-led changes in legislation resulting in stricter marketing laws, especially in regards to
targeted advertising aimed at youth. In the mid 1970’s, almost 30% of 12th graders reported smoking
cigarettes daily, and that number has decreased to only 4% in 2018. On the other hand, the rising
popularity of vape among teenagers has encouraged nicotine consumption through an alternate route
of administration: Teen e-cigarette users are more than 3 times more likely to start smoking cigarettes
compared to non e-cigarette users.
Cannabis
Cannabis is a drug that is derived from the flowering buds or other parts of the Cannabis sativa
plant. The most common route of administration is inhalation, or smoking. As cannabis becomes
decriminalized or outright legalized across the US, oral preparations are becoming more commonplace.
The effects of cannabis are often felt within minutes, and can include a sense of euphoria, relaxation,
distortion in the sense of perception, and potentially a lightness (high) or a heaviness of body (stoned).
Cannabis is currently classified as a Schedule I drug federally, but as of 2022, 23 states/territories have
legalized recreational use of cannabis, and only 12 states do not permit cannabis for medicinal use.
The stigma surrounding the “evils” of cannabis use are largely a remnant of a morality propaganda
campaign driven by the publishing of such “educational” films as Reefer Madness in 1936. Much of
our current medical understanding of cannabis is that it is far less harmful than once believed, although
it does have a negative impact on the developing brain, as significant adolescent exposure to cannabis
increases the likelihood of developing psychiatric conditions such as schizophrenia later in life.
Opioids
Opioids are a class of drugs, either natural or synthetic, that can bind to and activate the body’s
endogenous complement of opioid receptors. The most well known natural opioid is opium, but the
other popular drugs of this class include the street drug heroin or the medicinal substances morphine
or fentanyl. Derived from the poppy plant, evidence of human use of opioids for both medical and
recreational use dates back more than 5000 years to ancient Sumer. The body naturally produces
opioid substances, endorphin being the most well known. Activation of opioid receptors, either by
endogenously produced opioids or exogenous opioids, induce a potent analgesia, dampening the
sensation of pain. Opioid drugs are the current gold standard for pain relief. They also cause sedation
and feelings of euphoria.
Heroin and morphine are frequently taken via intravenous injection, but many prescription opioids,
particularly for long-term pain conditions, are consumed orally. Over the past 20 years, there has
been a dramatic increase in the number of opioid overdose related deaths. The most recent spike in
deaths is due to overdose on synthetic opioids such as the extremely potent painkiller fentanyl, which
saw a 10-fold increase between 2013 and 2017. The so-called opioid epidemic kills an estimated 130
individuals from the United States each day.
Cocaine
Cocaine is a psychostimulant that is derived from the coca plant. The most common routes of
administration are insufflation (powder cocaine), inhalation (crack cocaine), but it can also be
solubilized and injected or taken via transbuccal and oral administration by chewing the leaves of
the plant. Cocaine is considered to be a potent sympathomimetic, meaning the substance activates
the sympathetic nervous system. Therefore, the physiology of a person on cocaine will be similar
to an activated “fight-or-flight” response: elevated heart rate and blood pressure, dilated pupils, and
increased respiration. Cocaine is a Schedule II substance. Although it has a very high addiction and
harm potential, it has a handful of medicinal applications as well. For example, it is a potent local
anesthetic and vasoconstrictor, so it is often used in surgery, especially in facial or nose surgeries.
Psychedelics
Psychedelics are the class of drugs formerly called hallucinogens. They can be either natural, such as
psilocybin which is derived from mushrooms, or man-made, in the case of lysergic acid diethylamide
(LSD). Psychedelics can cause a person to experience visual distortions, synesthesia, an altered sense
of self (ego dissolution), or sometimes a sudden connection with nature or a higher power. Although
psychedelics have been used for centuries in religious ceremonies, they have had a troubled history in
the US. The adoption of LSD (acid) by the counterculture movement during the 1970’s, secret CIA
funded research, and some loosely-regulated research ethics in the clinical setting all led to notoriously
negative press regarding the substance, tainted the general public opinion about this class of drugs.
Since 2010, there has been a growing body of evidence supporting the notion that psychedelics can
serve a therapeutic purpose, most notably in treating psychiatric conditions such as post traumatic
stress disorder, terminal illness-related depression, and drug addiction.
Comparing different drugs based on their potential for drug dependence and the amount of physical
harm that they cause reveals that drugs such as heroin are classified as more dangerous.
Figure 51.2. This

graph relates
how addictive a
drug is and the
physical harm
that it can
potentially
cause. A drug
that has a
stronger
possibility of
leading to drug
dependence
would be a “3”
on the y-axis of
this graph. A
drug that has a
stronger
possibility of
leading to
physical harm
would be a “3”
on the x-axis of
this graph.
Heroin has high
dependence
potential and
high potential to
cause physical
harm.
Biology of Substance Use Disorder
Brain Structures Important in Substance Abuse

Disorder
The mesolimbic dopamine pathway within the brain underlies reward (discussed in Chapter 49). As
a reminder, our reward signaling neural circuitry starts with the ventral tegmental area (VTA).
Located in the midbrain, the VTA contains neurons that synthesize the neurotransmitter dopamine
(DA). These VTA DA neurons send their axonal projections predominantly into two main areas.
The mesolimbic pathway consists of dopamine producing neurons that release dopamine onto the
cells in the nucleus accumbens (NAc), serving as the major pathway by which reward is mediated by
the brain. A separate group of dopaminergic neurons in the VTA project to the prefrontal cortex in the
mesocortical pathway. Drugs of abuse act on this pathway, causing drugs to cause heightened feelings
of reward, making it more likely that individuals engage with these substances.
Figure 51.3. The

ventral
tegmental area
(orange region)
releases
dopamine into
the nucleus
accumbens
(purple region)
via the
mesolimbic
pathway and
releases
dopamine into
the prefrontal
cortex (blue
region via the
mesocortical
pathway.
’Mesolimbic and
Mesocortical
Pathways’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
The nucleus accumbens can be subdivided into two portions called the nucleus accumbens shell
and nucleus accumbens core, each with their own circuitry and functions. The nucleus accumbens
shell functions in the “wanting” involved in drug addiction, as well as reward and reinforcement.
The nucleus accumbens core, however, functions in the motor functions related to reward and
reinforcement.
Plasticity
Plasticity in Dopamine Signaling

Using microdialysis, it is possible to measure the dopamine concentration in the NAc during
performance of some behavioral tasks. In studies with rats, engaging in rewarding activities such as
eating or sex results in increased release of dopamine in the NAc. Similarly we see that release of
dopamine into the human NAc is enhanced when we engage in activities we enjoy: binge eating
sugary stuff, having sex, even playing video games. Notably, many drugs of abuse such as cocaine or
amphetamine can induce a similar overflow of dopamine release when an animal is exposed to the drug.
Many drugs of abuse cause a sensitized increased in dopamine release, meaning that there is an
increase in dopamine release. Sensitization is a phenomenon in which there is an increased effect of
the drug with chronic exposure. The increased dopamine release at the nucleus accumbens causes an
increase in dopaminergic signaling, which can then lead to structural plasticity.
Structural Plasticity
Repeated administrations of multiple drugs of abuse causes cellular plasticity within the nucleus
accumbens and other structures within the reward pathway, by increasing dendritic spine density.
Dendritic spines are small protrusions off the main dendritic shaft of neurons. They serve as the
excitatory synaptic inputs for these cells, increasing excitability. Notably, though the increase in
dendritic spine density is observed following administration of multiple drugs of abuse, it is not a
universal structural change as opiates do not produce the same dendritic spine increase.
Figure 51.4.
Dendritic spine
density. Dendritic
spines are
excitatory
projections off
the main
dendritic shaft
and are found on
neurons
throughout the
brain, including
the reward
pathway.
Administration of
many drugs of
abuse results in
an increase in the
density of
dendritic spines
compared to
administration of
a control
substance like
saline. ‘Dendritic
spine density’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Actions of Drugs at the Synapse
Cocaine
Cocaine acts on a molecular level by functioning as a dopamine reuptake inhibitor, preventing the
transporter protein from clearing the neurotransmitters such as dopamine out of the synapse. This
leads to increased concentrations of dopamine in the nucleus accumbens and prefrontal cortex. This
increased dopamine results in a greater likelihood that the postsynaptic receptors get activated.
Amphetamine
Amphetamine acts similarly to cocaine at the synapse, blocking the dopamine transporter and
preventing reuptake into the presynaptic cell. Amphetamine can also mimic dopamine at the
dopaminergic synapses, binding to dopaminergic receptors.
Opioids
Opioids being to GABAergic neurons, causing them to hyperpolarize and suppress release of GABA
onto dopaminergic VTA neurons, disinhibiting the VTA neurons and increasing dopamine release
onto downstream targets.
Cannabinoids
Although the cannabis plant contains several chemical substances, the main psychoactive ingredient is
delta-9-tetrohydrocannabinol, or THC for short. THC, once it enters the bloodstream, is capable of
activating receptors that our body uses endogenously, called the cannabinoid receptors (CB receptors).
THC inhibits activity of GABAergic neurons that synapse on the dopaminergic VTA neurons,
disinhibiting the VTA neurons and increasing dopaminergic signaling.
Nicotine
Pharmacologically, nicotine has specificity as an agonist at nicotinic acetylcholine receptors (nAChRs),
excitatory ionotropic receptors that are expressed widely across the body. When nicotine binds to VTA
neurons,it activates the cells and causes an increase in dopamine release. Nicotine can also increase
glutamatergic activation originating from the prefrontal cortex onto VTA neurons. Collectively, this
acts to increase activity of VTA neurons and increase release of dopamine at downstream targets.
Alcohol
Ethanol is a tiny molecule with properties similar to water, meaning that ethanol can diffuse easily
through cell membranes. Whereas other chemical substances often have highly specific molecular or
protein targets, ethanol has several. Ethanol activates dopamine neurons within the ventral tegmental
area, causing an increase in dopamine release at the nucleus accumbens. Further, alcohol acts to
inhibit GABAergic neurons within the VTA, disinhibiting the dopamine neurons and increasing their
signaling.
Figure 51.5.
Model of how
different abused
drugs affect
dopamine
signaling in the
mesolimbic
dopamine
pathway.
Dopaminergic
neurons in the
ventral
tegmental area
project to the
nucleus
accumbens and
prefrontal cortex.
There are
glutamatergic
neurons within
the prefrontal
cortex project
back to the
ventral
tegmental area
dopaminergic
neurons.
GABAergic
neurons in the
ventral
tegmental area
also project to
the ventral
tegmental area
dopamine
neurons. Drugs
of abuse act at
different sites
within this
circuitry to
ultimately
increase
dopamine
signaling
between the
ventral
tegmental area
and the nucleus
accumbens. ‘Sites
of action for
different drugs of
abuse’ by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Tolerance and Sensitization
Tolerance
Pharmacologically speaking, tolerance can be defined as a decrease in the action of a drug due to repeat
exposure. This phenomenon happens as a result of homeostatic adaptations that the body undergoes
when it has been exposed to a substance for a prolonged period of time. In a person who is tolerant,
they must take progressively higher doses of the drug to experience a desired effect. For a drug naive
person, someone with no experience with the drug, a low dose of drug may have a strong effect.
However, for a person who has become tolerant to the effects of the drug, that same low dose will
produce a small drug effect.
There are different forms of tolerance that a person can experience. In metabolic tolerance, there is
a decrease in the amount of the drug that gets to the site of action. The body becomes more efficient at
eliminating the substance. Since exogenous substances usually get degraded by enzymatic reactions, an
increase in the activity or number of enzymes will decrease the amount of the substance that is capable
of acting.
The most common example of metabolic tolerance is seen in frequent alcohol use. The main
psychoactive substance found in alcoholic beverages like beer, wine, or liquor is ethanol. Our liver,
which filters circulating blood, contains enzymes which break down chemicals in the blood.
Specifically, the standard degradation process of ethanol uses two enzymes. The first of which, called
alcohol dehydrogenase, oxidizes molecules of ethanol into the toxic byproduct acetaldehyde. The
second step uses the enzyme acetaldehyde dehydrogenase to convert the acetaldehyde into acetic acid,
a generally harmless compound (the same chemical structure as vinegar) that is used by the body
in a biochemical reaction that drives the citric acid cycle. Because most alcohol is taken via oral
consumption, ethanol is subject to first-pass metabolism before reaching systemic circulation.
However, after prolonged exposure to alcohol, the body undergoes homeostatic compensatory
changes. The two liver enzymes responsible for degradation of ethanol increase in either function
or amount. When these metabolic enzymes become upregulated, the circulating ethanol in the
bloodstream becomes broken down more rapidly and more efficiently, resulting in a decrease in the
amount of ethanol that reaches general circulation, and consequently, the brain. This explains why
people who have never drank alcohol (low amounts / function of enzymes) may experience a stronger
drug effect compared to someone who drinks regularly. It also accounts for why there is some genetic
variation between a person’s sensitivity to alcohol’s intoxicating effect, as enzyme efficiency is partially
encoded by genetics.
Another way by which tolerance can manifest itself is through changes at the level of the molecular
components of cells. This type of tolerance is called cellular tolerance. This tolerance leads to a
decreased sensitivity to the substance. Cellular tolerance can be characterized by a decrease in receptor
expression after chronic exposure to an agonist. When the opioid etorphine is present in the body,
it activates the opioid receptors. Frequent exposure to etorphine causes a molecular change in the
receptors, which leads to internalization, the process by which cell surface-expressed receptors become
taken into the cell. Since etorphine is only able to activate receptors on the surface, these internalized
receptors diminish the strength of the etorphine signal, which manifests as tolerance. By
downregulating the number of receptors that are expressed on the surface, the cell reacts to the
environment by becoming less responsive in the face of drug.
Sometimes, the body physiologically prepares for the drug effect before the drug is even present. The
way it does this is to initiate an opposite somatic effect from what the drug is expected to do. This
“reverse” effect is likely a protective mechanism to minimize the drug effect, and these anticipatory
bodily changes can be precipitated by drug-associated cues. For example, heroin causes analgesia. Cues
that predict administration of the drug, such as preparation for an intravenous injection or being
in a physical environment where drug is frequently administered, can cause the reverse physiological
effect, hyperalgesia. Because of this, a person may need to take more drug to overcome the anticipatory
changes. This unconscious form of learning leads to a form of tolerance that is dependent on Pavolvian
cues; this is called conditional tolerance. If not accounted for, conditioned tolerance can have
potentially lethal side effects. A clinical description (Siegel, 2001) of a patient with pancreatic cancer
describes one such “failure” of tolerance. Bedridden and suffering from tremendous pain, a man was
regularly given morphine in his bedroom, dark and filled with humming apparatus for his medical
care. As expected, he developed a tolerance towards the morphine, requiring higher and higher doses
in order to inhibit pain. One day, he went into the brightly lit living room to receive his normal dose of
painkiller. Soon after, he died from opioid overdose.
Sensitization
Sensitization is a phenomenon related to tolerance, but the polar opposite: instead of a drug effect
being lessened after chronic exposure, a sensitized person experiences an increase in drug effect.
Nicotine as well as many psychostimulants such as cocaine or amphetamine can produce
psychomotor sensitization after repeated dosings. Psychomotor sensitization can be measured
through crossovers (how often the animal runs across the midline of the cage). Animals displaying
sensitization will show significantly more crossovers compared to non-sensitized animals. This
sensitization can be long-lasting, remaining for up to one year following the last dose of drug.
Using Animal Models to Study Abused

Substances
“Why do some people transition from user to addict?” Many people are able to use the most addictive
substances such as cocaine or heroin casually without ever becoming an addict. For these people,
their drug use pattern can remain recreational, even though they may use the substance frequently
and report a great sense of satisfaction when they use the drug. On the other hand, there are some
people who have a very high propensity to quickly becoming a pathological addict. One of the big
challenges in drug abuse research is to understand the differences between these two populations –
what factors, either environmental or genetic, are protective and prevent the transition from casual
drug user to compulsive drug addict? And what are some ways we can use this knowledge to help
addicts find ways to break their pattern of harmful compulsive drug seeking and drug use? One
possible way to get at the answer is to create animal models of drug dependence and try to determine
the nature of the underlying biological changes that contribute to the addiction-like pattern: Do some
cellular circuits change permanently? Are certain genes or proteins upregulated or downregulated?
Can pharmacological or behavioral interventions change drug-taking or drug-seeking behavior?
Self-Administration
One of the most instructive non-human animal models of drug use disorder is called self-
administration. In a self-administration experiment, the subject (usually a monkey, rat, or mouse)
gets a surgical implantation of an indwelling intravenous tube that is connected to a pump. Then, the
subject is put into an operant conditioning chamber. When they press the lever or poke their nose
into a hole, the pump triggers, resulting in an infusion of drug directly into the bloodstream. This
experimental paradigm closely mimics human drug seeking behavior. For example, rats and mice will
very quickly learn to press on the levers for intravenous infusions of cocaine and morphine. Many of
them will give themselves drugs even despite severe adverse consequences, such as enduring foot shock
– arguably similar to human behavioral patterns where addicts may do all kinds of behaviors for access
to drug. Monkeys, if given the option to self administer for cocaine, must be restricted in the amount
of drug they receive: if given unrestricted access, they give themselves so much cocaine that they have
seizures and die.
Figure 51.6.
Self-Administrati
on. In this
apparatus, called
an operant
conditioning
chamber, an
animal is trained
to press a level or
poke their nose
in a hole in order
to receive an
infusion of a
drug.
Conditioned Place Preference

A second experimental test created to study drug abuse is called conditioned place preference (CPP)
(also discussed in Chapter 50). A CPP test works on the principle that animals learn to associate
certain environments with positive feelings, and will prefer to spend significantly more time in those
environments. To conduct CPP, a three-room testing chamber is used. This experimental chamber has
two large rooms, each with characteristics that are unique to that room. For example, one room may
have mesh flooring with dark-colored walls, while the other room may have woodchip flooring with
light-colored walls. The two rooms are connected by a smaller, neutral room, and the rooms can be
isolated from another by means of a dividing door. To conduct CPP, an animal will be given the drug
of interest, then placed into one of the two rooms, where they are free to explore the room, but cannot
leave. As a control, at a different time, they will be injected with an innocuous solution and placed
into the other room. On test day, the animal will be put into the neutral middle room, and given the
opportunity to move freely between the two chambers. If the animal developed positive associations
with the room where they experienced the drug effect, then they will choose to spend significantly
more time in that room. On the other hand, if they experienced negatived conditioned place feelings
associated with that room, they will avoid being in the room. Whereas self-administration assesses
“drug seeking,” CPP measures “drug liking.”
Figure 51.7.
Conditioned
Place Preference.
Conditioned
place preference
is a test that uses
a three chamber
testing
apparatus. The
two testing
compartments
have different
colored walls and
different
textured floors.
During a
pre-test, the
animal roams the
entire apparatus
and the amount
of time spent in
each
compartment is
recorded. During
conditioning, one
environment is
paired with a
drug, and the
other
compartment is
paired with
saline. After
conditioning, the
amount of time
spent in each
chamber is
recorded. If a
drug is
rewarding, the
amount of time
spent in the
conditioning
compartment
will increase. If a
drug is not
rewarding, then
the amount of
time spent in the
compartment
will decrease or
stay the same.
Hypotheses to Explain Substance Abuse

Disorder
Below are three major biology-driven hypotheses that have been proposed to explain drug addiction.
It’s important to note that none of them tell the complete story – otherwise drug addiction will have
already been cured! Also, these theories are not mutually exclusive from one another. A likely answer to
curing addiction probably incorporates some combination of these approaches in addition to societal
policy changes.
Hedonia Hypothesis
What drives addicts to behave in the way they do? One of the earlier theories put forward to explain
addiction is the hedonia hypothesis proposed in the late 1970s by Roy Wise. The hedonia hypothesis
hinges on the assumption that dopamine is the “pleasure neurotransmitter,” and that any substance
or behavior that increases dopamine will be desirable. The theory is supported by some behavioral
data. For example, using microdialysis, it was found that dopamine release by the mesolimbic pathway
increases in the presence of many drugs of abuse. Additionally, administration of dopamine
antagonists are able to block some drug taking behavior. This dopamine-centric approach to drug use
disorder was so influential that pharmaceutical companies, while developing novel antidepressants,
quickly dismissed drugs that increased dopamine out of fear of the risk of addiction potential.
Despite the attractiveness of the straightforward “pleasure neurotransmitter” model, the dopamine
hypothesis does not give us the whole picture. For one, dopamine release at the mesolimbic pathway is
only notable for psychostimulant drugs, such as cocaine and methamphetamine. But there are plenty
of stimulants that still produce the significant overflow of dopamine without causing any rewarding
sensation. Furthermore, if dopamine is the signal that mediates the positive hedonic feelings, one
might predict that by blocking dopamine receptors chronically, you can also prevent long-term cocaine
addiction, but this therapeutic strategy isn’t very useful. Many of the most popular misconceptions
about dopamine that you frequently see in the media take this early hypothesis as the truth. It even
was featured as the cover of a 1997 Time magazine. That same year in an interview with the original
proponent of the hedonia hypothesis published in Science, Roy Wise agreed with recent results that
his model was incomplete.
Incentive Sensitization Model

A more recent theory to explain drug addiction is a model developed by researchers from the University
of Michigan, Terry Robinson and Kent Berridge. Under their model of drug use, initially a person
takes a drug because they like the positive, euphoria-producing side effects of the substance. But after
chronic exposure to the substance, the person becomes fixated on obtaining the drug rather than how
the drug makes them feel. At this point, they continue to use the drug even though they no longer
enjoy the drug to the same degree that they once did. Instead of “liking” the drug, the addict fixates on
“wanting” the drug. The incentive sensitization model suggests that the drug-exposed brain develops
an increased sensitivity to drugs, but also more importantly, drug related cues.
Pavlovian learning takes place when an addict takes a drug. Each drug has an associated set of
cues, and reexposure to those cues can trigger relapse. Over repeated pairings, those cues trigger a
progressively stronger response. Their model accounts for why it is difficult to remain abstinent from
an addictive drug for long durations of time. Despite a decrease in drug seeking behaviors, many addicts
eventually relapse, or return to the previous destructive pattern of drug use. One phenomenon that
makes staying drug free difficult is the observation that prolonged abstinence can increase the intensity
of drug cravings. This is called incubation of craving, and is seen with several different classes of drugs,
notably, nicotine.
Brain Disease Model of Addiction

Much of our current understanding of addiction is based on the principles that repeated drug use leads
to biological changes in the brain that resemble aberrant learning. This model for drug addiction is
called the Brain Disease Model of Addiction. Genetics has a strong influence on the likelihood that
someone develops a disease, and addiction is no different. Inherent risk-taking behaviors, magnitude
of a drug effect, and risk of relapse are all modified by genetics. One of the major implications of this
model is that compulsive drug use and seeking is not a choice that a person makes. Addicts are not the
way they are because of some poor moral guidance or a weakness of willpower. Addicts compulsively
seek drug because of some underlying circuitry of their brain that makes cessation difficult.
One substantial accomplishment of this model is that public policy has adjusted to increase access to
mental health resources for addicts to help cover the financial challenges of recovery programs (Mental
Health Parity and Addiction Equity Act of 2008), and to improve the outcomes of nonviolent drug
offenders as they reintegrate with society outside of prisons. This model is not without fault, however.
The major criticism of this framework to explain compulsive drug use is that most people stop their
addictive use patterns by themselves spontaneously without any treatment. Another harm of the Brain
Disease Model of Addiction is that it puts heavy emphasis on biochemical therapies rather than on a
public policy approach to drug addiction treatment. Lastly, this model has not produced a successful
therapeutic strategy to help with addiction.
Attributions
Key Takeaways
• The DSM-5 is used for diagnosing substance abuse disorder.

• Drugs of abuse act on the mesolimbic dopamine pathway to make drugs rewarding.
• Increased dopamine signaling can lead to structural plasticity, such as increased
dendritic spine density.
• Drugs of abuse have different sites of action within the mesolimbic dopamine
pathway to ultimately cause an increase in dopamine signaling.
• Drug tolerance causes a decrease in drug action with repeated use, whereas drug
sensitization causes an increase in drug action with repeated use.
• Animal models of self-administration and conditioned place preference are used to
better understand mechanisms of drug action.
• There is not one complete hypothesis of drug addiction.
Media Attributions
• DSM5 © Yoshikia2001 adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution

ShareAlike) license
• Graph comparing dependence and physical harm © Apartmento2 adapted by Valerie Hedges is
• Dendritic spines © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Drug action © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Self Administration © Jinrikisha Amufabalo adapted by Valerie Hedges is licensed under a
Public Domain license
• CPP © NeuroFall2021 adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
762 | SEXUAL DIFFERENTIATION
52.
SEXUAL DIFFERENTIATION
Sexual differentiation is the process by which a person

develops into either a male or a female. For the purpose of
Resources this chapter, the content will be based on a male / female
binary to introduce the basic concepts of reproductive
development. However, it is important to recognize that
in real life, chromosomal sex, physical sex, and gender exist
• Glossary terms
on a continuum and cannot always be simplified into a
• Key Takeaways
two-structure system.
Gender is a cultural variable with behaviors and
attributes that are associated with being masculine or
feminine and is determined by interactions between genes, hormones, life experiences, and cultural
expectations.
During development, the body and the brain undergo either A) feminization and de-
masculinization or B) masculinization and de-feminization. In most cases, the differentiated brain will
lead to behaviors that correspond to the differentiated gonads.
SEXUAL DIFFERENTIATION | 763
Figure 53.1. In
most cases,
human females
have feminized
and
desmasculinized
brains and bodies
whereas human
males have
masculinized and
defeminized
brains and bodes.
‘Gender Icons’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Chromosomal Sex
In humans, DNA is organized into 46 chromosomes. One set of 23 chromosomes comes from the
mother and the other set comes from the father. Twenty-two pairs are called autosomal
chromosomes. These chromosome are similar in length and have the same genes present at the same
location regardless of if they are received from the mother or father. However, for all genes, the allele,
or version, present for each gene may be different from each parent. The last pair of chromosomes
is responsible for determining if an individual becomes a male or female; these are called the sex
chromosomes. In humans the sex chromosomes are named either X or Y.
Figure 53.2.
Humans have 23
pairs of
chromosomes,
making 46 total.
22 pairs are called
autosomal and
have similar
structure from
each parent. The
final pair are the
sex
chromosomes
and determine if
the individual is a
male or female.
Sex
chromosomes
are named either
X or Y.
‘Chromosomes’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Fertilization occurs when a sperm cell from the father fuses with an egg cell from the mother. All
egg cells contain one X sex chromosome. Sperm cells contain either one X or one Y chromosome,
which means chromosomal sex in humans is determined by the sperm. If a sperm carrying an X
chromosome fertilizes an egg, the resulting fetus will be XX and a female, whereas if a sperm carrying a
Y chromosome fertilizes an egg, the resulting fetus will be XY and a male.
Figure 53.3. The

combination of
an X-containing
sperm cell and an
X-containing egg
cell will result in a
XX individual
who will develop
as a female. The
combination of a
Y-containing
sperm cell and an
X-containing egg
cell will result in a
XY individual
who will develop
as a male.
‘Fertilization’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
International
License.
Gonadal Differentiation
The presence of the Y chromosome signals for the formation of testes in the fetus. The Y chromosome
is much smaller than the X chromosome (50 genes vs ~800 genes). There is gene on the Y chromosome
called the sex-determining region (SRY) of the Y chromosome. The SRY gene is required for
masculinization of the embryonic gonads. Other genes on the Y chromosome are also important in sex
determination and in male physiology, however we will focus on the SRY gene.
The SRY gene encodes for a protein called the testis-determining factor (TDF), which causes the
embryonic gonads to differentiate into the testes. The testes then begin secreting both testosterone
and a hormone called the Müllerian inhibiting substance (MIS). Testosterone causes Wolffian ducts
to develop into the vas deferens, seminal vesicles, and epididymis. MIS causes the Müllerian ducts to
degenerate. The presence of testosterone also results in the development of the prostate gland and
penis.
Because XY males only have one copy of the X chromosome, they are more at risk for X-linked
diseases.
Figure 53.4. A.
The
undifferentiated
gonadal system
is the same for
both sexes. In
males, the SRY
gene, located on
the Y
chromosome, is
activated during
development,
producing
testis-determinin
g factor, which
results in the
gonads
becoming testes.
B. The testes
begin releasing
testosterone and
Müllerian
inhibiting
substance, which
cause the
Wolffian ducts to
become the vas
deferens, seminal
vesicles, and
epididymis and
cause the
Müllerian ducts
to degenerate. C.
The presence of
testosterone also
causes the
development of
the penis and
prostate gland in
the fully
developed
system. ‘Male
Gonad
Differentiation’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
In females, when the SRY gene and secreted hormones are absent, the gonads differentiate into the
ovaries, the Müllerian ducts develop into the fallopian tubes, uterus, and vagina, and the Wolffian ducts
degenerate.
Figure 53.5. A.
The
undifferentiated
gonadal system
is the same for
both sexes. In the
absence of the
SRY gene during
the 6th to 12th
week of
gestation, the
gonads become
the ovaries. B.
The ovaries do
not produce any
hormones during
development
which causes the
Müllerian ducts
to become the
fallopian tubes,
uterus, and
vagina, and the
Wolffian ducts
degenerate. C. In
the fully
developed
system, the
cervix is the
lower part of the
uterus, which
separates the
uterus from the
vagina. ‘Female
Gonad
Differentiation’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Chromosomal Abnormalities and Effects on Sex
There are a number of disorders that result from chromosomal abnormalities. In Klinefelter
Syndrome, individuals have an XXY genotype. Due to the presence of a Y chromosome,
individuals with Klinefelter Syndrome have a “male” appearance. The additional X
chromosome causes the individual to have less body hair, small testes, wide hips, long arms
and legs, more breast tissue, and a female pubic hair pattern. The additional X chromosome
causes many individuals with Klinefelter’s Syndrome to be infertile.
In Jacobs Syndrome, also called XYY Syndrome, individuals have an XYY genotype.
Again, due to the presence of a Y chromosome, these individuals typically have a “male”
appearance. Individuals may also be taller than average and have an increased risk of
learning disabilities. Psychological studies indicate that individuals with XYY Syndrome may
have difficulty with impulse control and emotional regulation. Historically, increased
testosterone is correlated to increased aggressive behavior in incarcerated males with XYY
Syndrome.
In Trisomy X, also called XXX Syndrome, individuals have an XXX genotype. These
individuals have a “female” appearance. Fewer than 10% of individuals that have XXX
Syndrome are aware that they have it. Symptoms include learning disabilities, wide-spaced
eyes, and increased height. Individuals have an increased risk for early menopause.
In Turner Syndrome, individuals have an XO genotype. The “O” indicates the absence of a
chromosome, therefore rather than 2 sex chromosomes, individuals with Turner Syndrome
only have 1 X chromosome. The lack of Y chromosome leads to these individuals having a
“female” appearance. Other symptoms associated with Turner Syndrome include short
stature, webbed neck, low-set ears, and a receding jaw. Due to only having one X
chromosome, ovaries typically do not form, or form abnormally, and breasts do not develop.
Hormone therapy is necessary for the development of a menstrual period and breasts.
There is not a corresponding YO genotype, because at least one X chromosome is necessary
for life.
Key Takeaways
• During development, the body and the brain undergo either A) feminization and de-
masculinization or B) masculinization and de-feminization
• The sex chromosomes, X and Y, make up one pair of the 23 total pairs of
chromosomes in humans
• Females are genetically XX and males are genetically XY
• The SRY gene on the Y chromosome is responsible for the development of the male
reproductive system
• In the absence of hormones, the female reproductive system develops
Attributions
Media Attributions
• Gender Icons © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial

ShareAlike) license
• Chromosomes © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Fertilization © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Male Gonad Differentiation © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Female Gonad Differentiation © Casey Henley is licensed under a CC BY-NC-SA (Attribution
SEX STEROID HORMONES | 773
53.
SEX STEROID HORMONES
Control of gonadal hormone release relies on activation

of the hypothalamic-pituitary-gonadal (HPG) axis.
Gonadal hormones are important for development of the Resources
body and brain, changes during puberty, and the
activation of some behavior in adulthood like
reproductive behavior and aggression.
• Glossary terms
• Key Takeaways
Hypothalamus
As a refresher, the hypothalamus, which is located
inferior to the thalamus, integrates information from many regions of the central nervous system and
maintains homeostasis in the body. The hypothalamic regulation of gonadal hormones and sex
behavior is managed via hormone release by the pituitary gland.
774 | SEX STEROID HORMONES
Figure 52.1. The

pituitary, shown
in green in a
mid-sagittal
section, lies just
below the
hypothalamus,
shown in blue.
‘Hypothalamus
and Pituitary’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
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License.

View the pituitary using the BrainFacts.org 3D Brain
Hormone Release
Gonadal hormone release relies on anterior pituitary function. In the hypothalamus, the parvocellular
neurosecretory cells release a hormone called gonadotropin-releasing hormone (GnRH) into the
hypophyseal portal circulation. When GnRH reaches the anterior pituitary, it causes the endocrine
cells of the pituitary to release luteinizing hormone (LH) and follicle-stimulating hormone
(FSH) into the general circulation.
Figure 52.2. In
the HPG axis, the
hypothalamic
parvocellular
neurosecretory
neurons release
gonadotropin-rel
easing hormone
(GnRH) into the
hypophyseal
portal circulation,
causing the
hormone-releasi
ng endocrine
cells in the
anterior pituitary
to release
luteinizing
hormone (LH)
and
follicle-stimulatin
g hormone (FSH).
‘LH and FSH
Release’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
The LH and FSH travel through the circulatory system and can act on the gonads, either the testes in
males or ovaries in females. In response to the pituitary hormones, the testes release testosterone, an
androgen, and the ovaries release estradiol, an estrogen, into the blood stream. After puberty, the
LH and FSH are also critical for the maturation of sperm and egg cells.
Figure 52.3. The

gonads release
either
testosterone
(testes) or
estradiol
(ovaries) into the
bloodstream in
response to
release of LH and
FSH by the
anterior pituitary.
‘HPg Axis’ by
Casey Henley is
licensed under a
Creative
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Sex Steroid Hormone Synthesis

Sex steroid hormones are produced by the gonads (ovaries or testes). All sex steroid hormones are
originally generated from cholesterol. Males typically have higher concentrations of androgen sex
steroid hormones, such as testosterone and 5-alpha-dihydrotestosterone (DHT). Androgens are
primarily released from the testes. Females typically have higher concentrations of estrogens (such
as estradiol) and progesterone that are primarily released from the ovaries. Importantly, males also
have estrogens and progesterone, and females have androgens. The only difference is the relative
concentration between males and females.
In the synthesis pathway, enzymes are responsible for converting one hormone into another
hormone. The enzyme 5-α reductase converts testosterone into DHT. DHT is important for
masculinization of the body structures. Through just one chemical reaction, the enzyme aromatase
converts testosterone into estradiol.
Figure 52.4 Sex

steroid hormone
synthesis. The
sex steroid
hormones are all
synthesized from
cholesterol.
Through a
number of
chemical
reactions,
cholesterol is
converted into
progesterone.
Through a series
of additional
chemical
reactions,
progesterone is
converted into
testosterone.
One chemical
reaction converts
testosterone into
estradiol through
the actions of the
enzyme
aromatase. One
chemical reaction
converts
testosterone into
dihydrotestoster
one (DHT)
through the
actions of the
enzyme 5-α
reductase.
Hormone Action
Once the gonadal hormones are synthesized and enter the circulation, they are able to act on cells that
express either androgen receptors or estrogen receptors. Testosterone and estradiol are steroid
hormones that can affect target cells through two different mechanisms:
1. Classical mechanism– Steroid hormones like testosterone and estradiol are able to pass
through the phospholipid membrane of a neuron because they are lipophilic. Some neurons
express receptors for these hormones. Androgen receptors bind androgens like testosterone
while estrogen receptors bind estrogens like estradiol. When a hormone binds to a receptor in
the neuron, the hormone-receptor complex dimerizes and moves into the nucleus where it can
bind to specific promoter sites on the DNA and act as a transcription factor to turn on or off
certain genes (genomic effects).
2. Rapid mechanism– Sex steroid hormones can also bind to membrane-bound receptors and can
exert more rapid effects within the cell.
Animation 52.1. Steroid hormones can cross the phospholipid bilayer and bind to hormone receptors.
The receptors dimerize, move to the nucleus, and interact with DNA, altering transcription of certain
genes. ‘Estradiol Action’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Commercial Share-Alike (CC BY-NC-SA) 4.0 International License.
The testes secrete testosterone during the prenatal critical period. The effect of this testosterone is
to masculinize and defeminize the brain, body, and behavior, and this is accomplished through the
transcription of a specific set of genes. However, many of those genes are not transcribed by the action
of androgen receptors interacting with the DNA. Instead, when testosterone enters the cell, it does
not always bind to androgen receptors. Some neurons also express the enzymes 5-α reductase and
aromatase that break testosterone down into its metabolites, DHT and estradiol.
Animation 52.2. After testosterone enters the cell, if it does not bind to an androgen receptor, it can
be metabolized by enzymes in the cell. 5-α reductase converts testosterone into dihydrotestosterone.
DHT, like testosterone, can bind to and activate androgen receptors. The enzyme aromatase converts
testosterone into estradiol. Estradiol is an estrogen and can bind to and activate estrogen receptors.
During developmental critical periods, the aromatization of testosterone to estradiol leads to the
transcription of masculinizing genes in some animals like rodents. ‘Aromatization’ by Casey Henley is
In some mammals, like rodents, this conversion of testosterone to estradiol through the actions
of the enzyme aromatase is the main process by which neurons and the brain are masculinized. The
estrogen receptors cause the transcription of masculinizing genes. Therefore, somewhat surprisingly,
even though estrogen is typically thought of as a ‘female’ hormone, its actions during development are
responsible for much of the masculinization that occurs in the brain in some animals.
The brain is by default “female” during development, and requires the testosterone secreted by the
testes and converted into estradiol for “male” development. It should be noted, though, that estrogen
does not appear to have these same masculinizing effects during human development.
Key Takeaways
• The hypothalamus directly controls release of gonadal hormones by controlling

hormone release from the anterior pituitary
• The hypothalamus releases gonadotropin-releasing hormone (GnRH)
• The anterior pituitary releases luteinizing hormone (LH) and follicle-stimulating

hormone (FSH)
• The ovaries release estradiol
• The testes release testosterone
• Gonadal hormones bind to receptors and alter DNA transcription
Attributions
Media Attributions
• Hypothalamus Pituitary © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• LH and FSH Release © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• HPG Axis © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Sex steroid hormone synthesis © BorisTM, Rhododendronbusch, Klaus Hoffmeier,
NEUROtiker, Aethyta adapted by Valerie Hedges is licensed under a Public Domain license
ORGANIZATIONAL AND ACTIVATIONAL EFFECTS OF STEROID HORMONES | 781
54.
ORGANIZATIONAL AND ACTIVATIONAL

EFFECTS OF STEROID HORMONES
In addition to differentiating the reproductive duct

system, the presence or absence of gonadal steroid
hormones during development also differentiates the rest
of the body, including the brain. Testosterone causes the Resources
brain, body, and behavior of the individual to be
masculinized and defeminized. The quiescent ovaries do
not release hormones which causes the brain, body, and
behavior of the individual to be feminized and • Glossary terms
demasculinized. • Key Takeaways
782 | ORGANIZATIONAL AND ACTIVATIONAL EFFECTS OF STEROID HORMONES
Figure 54.1.
Testosterone
presence during
development
masculinizes and
defeminizes the
brain, body, and
behavior. No
hormone
exposure during
development
feminizes and
demasculinizes
the brain, body,
and behavior.
‘Developmental
Hormones’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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Sexual Dimorphisms
In the brain, gonadal hormones bind to receptors to alter neuronal function and overall behavior. In
many species, males and females have different roles in sexual reproduction when it comes to finding
a mate, copulation, birth, nursing, and nurturing offspring. It follows that there are differences in
structure and function between male and female brains.
A sexual dimorphism is a distinct difference in the size or appearance of a structure between the
sexes of an animal. In rodents, a sexual dimorphism has been described in the sexually dimorphic
nucleus of the preoptic area (SDN-POA) within the hypothalamus. The SDN-POA is generally
5-8 times larger in male rodents than it is in female rodents.
Figure 54.2
SDN-POA. A
coronal (frontal)
section of a
rodent brain
reveals the
hypothalamus on
the ventral
surface of the
brain
surrounding the
third ventricle.
The sexually
dimorphic
nucleus of the
preoptic area
(SDN-POA) is a
nucleus within
the
hypothalamus.
This nucleus is
sexually
dimorphic in
rodents and is
5-8x larger in
males than in
females.
‘SDN-POA’ by
Valerie Hedges is
licensed under a
Creative
Commons
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BY-NC-SA) 4.0
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Are there sexual dimorphisms in the human brain? This answer is not clear. There may be differences
in structures, but the differences are small and controversial. A set of nuclei within the human brain
has been studied with regard to sexual dimorphisms called the interstitial nuclei of the anterior
hypothalamus (INAH). Within this collection of nuclei, INAH-1, INAH-2, and INAH-3 have
been reported to be larger in men than in women, with INAH-3 having the largest difference. The
function of INAH in sexual behavior is still not well-understood. Importantly, there is a large degree
of variability between human brains. This means that even when a sexual dimorphism is observed it
is being observed “on average” across many brains. Within this comparison group, there will be brains
that do not show a sexual dimorphism or potentially show the exact opposite difference in size.
Critical Period
The hormonal effects of secreted testosterone on the brain must take place during a specific time in
development, called a critical period. Organizational effects of hormones lead to major, generally
irreversible, aspects of cell and tissue differentiation. Organizational effects take place during critical
periods like prenatal development and puberty.
In adulthood, the same hormones trigger physiological or behavioral responses called activational
effects that are reversible and short-lived.
Figure 54.3.
Hormones can
have
long-lasting,
organizational
effects when
present during
critical periods
such as during
the prenatal
period or
puberty. During
these critical
periods,
hormones will
alter the
structure of the
nervous system,
setting up cells
and circuits
needed to display
sex-typical
behaviors later in
life. Those
sex-typical
behaviors are
then activated in
adulthood by the
presence of
gonadal
hormones.
‘Organizational
versus
Activational’ by
Casey Henley is
licensed under a
Creative
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Attribution
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Organizational Effects of Steroid Hormones

The early role of testosterone is an organizational effect and results in a permanent change in the
nervous system and therefore behavior. The androgens released by the testes enter cells of the SDN-
POA and are converted into estradiol through the actions of the enzyme aromatase. The estradiol
within the SDN-POA acts to masculinize the structure of the SDN-POA by preventing cell death,
causing the SDN-POA to be larger in males than in females. Due to the female ovaries being inactive
during development (do not secrete estradiol), the female SDN-POA has a smaller, feminized structure
due to the normal cell death that occurs during development. If testosterone is experimentally
administered to a female during the organizational critical period, the SDN-POA will be masculinized
and larger, however if the testosterone is experimentally administered to a female after the
organizational critical period, the structure of the SDN-POA will remain small and feminized.
Figure 54.4.
Testosterone
during the critical
period
masculinizes the
SDN-POA. A.
During the critical
period of male
development, the
testes release
testosterone.
This testosterone
is aromatized
into estradiol in
the rodent brain,
and estradiol
masculinizes the
SDN-POA
structure,
causing it to be
larger. B. During
female
development, the
ovaries are
quiescent and do
not release any
hormones. The
lack of hormones
causes the
structure of the
SDN-POA to be
feminized and
smaller. C. If
testosterone is
administered to
female rodents
outside of the
critical period of
development, the
testosterone will
not cause a
change in
SDN-POA
structure. D.
However, if
testosterone is
administered to
females during
the critical
period, the
structure of the
SDN-POA is
masculinized and
larger, similar to
the structure of
the SDN-POA in
typical males.
‘SDN-POA
testosterone’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
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BY-NC-SA) 4.0
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License.
Hormone change in Humans: Organizational Effects
Clearly, the presence of hormones during the organizational critical period is critical for the
masculinization of the rodent brain. Below are examples of conditions in humans that
demonstrate the organizational effects of gonadal steroid hormones on both the brain and
body.
Androgen Insensitivity Syndrome (AIS) (also known as Testicular feminization

syndrome) affects the development of the reproductive organs. Individuals with this
syndrome do not have functional androgen receptors. Therefore, circulating androgens

cannot bind to androgen receptors which affects the development of secondary sexual
characteristics, causing these individuals to have a feminized appearance. They have an XY
genotype but have genitalia that look female at birth, with the presence of a vagina, but do
not have ovaries or a uterus. As a result, they do not menstruate and cannot become
pregnant. Due to the presence of the Y chromosome, individuals with AIS have
undescended, but typically functional testes. Many XY individuals that have AIS only
discover their syndrome during puberty when they do not experience their first menses.
Upon understanding their biology, many still prefer to call themselves women, dress like
women, and choose men as their sexual partners.
Congenital adrenal hyperplasia (CAH) affects individuals with an XX genotype. During

development, the adrenal glands of these individuals produce an excess of androgens. The
higher circulating androgen levels results in masculinization of the external genitalia to
varying degrees resulting in intersex conditions with ambiguous genitalia structure. These
individuals still have ovaries and do not have testes (due to the lack of Y chromosome). In
many cases, these individuals are treated medically with hormones and raised as female.
Lastly, a group of individuals in the Dominican Republic referred to as the “Guevedoces” are
XY genotypic individuals that are born with a feminized appearance and undescended
testes that are assigned a female sex at birth. These individuals have a deficiency in the
activity of the enzyme 5-α-reductase. 5-α-reductase is typically responsible for converting
testosterone into dihydrotestosterone (DHT), the hormone that is responsible for the
development of male external genitalia. However, at puberty there is a surge in
testosterone in these individuals that overcomes the 5-α-reductase enzyme deficiency,
allowing in the growth of male sex organs. This causes these individuals that were raised as
females to start growing a penis. “Guevedoces” literally translates to “penis at 12 years” in
Spanish. This phenomenon has also been observed in a small population with Papua New
Guinea. Culturally, this has major implications in gender roles for these individuals as only 2
genders are typically recognized, though they are described as a third sex.
Activational Effects of Steroid Hormones

Activational effects of gonadal steroid hormones occur during adulthood and are short-lived and
reversible. Examples of activational effects of gonadal steroid hormones include sexual behavior
patterns and ovulation. Removal of the activating hormone will cause the behavior to stop, but
replacement later will cause the response to begin again because the brain has previously been
organized to produce those behaviors when hormones are present. Interestingly, maternal and paternal
behavior have both been shown to change the brain in various ways such as changes in dendritic
branching and somatosensory maps through experiential plasticity.
The role of activational hormones can be demonstrated by adult castration in male rats. Healthy
males with intact testes will show sexual behavior when placed with a female rat. Castration, the
removal of the testes, will cause males to stop showing sexual behavior because the activating hormone,
testosterone, is no longer present. However, if the castrated males receive testosterone replacement,
they will resume showing sexual behavior. The sexual behavior brain circuit was organized during
development by exposure to gonadal hormones, and in adulthood that circuit can be activated by
testosterone. The adult behavior can only be seen when the activating hormone is present.
Figure 54.5
Removing
testosterone by
castrating an
adult male rat
will decrease the
amount of sexual
behavior
displayed
because the
hormone can no
longer activate
sexual behaviors
(solid orange and
dotted blue
lines). However,
if the castrated
animal is treated
with
testosterone,
sexual behavior
returns (solid
orange line).
‘Castration
Effects’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
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BY-NC-SA) 4.0
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Key Takeaways
• Testosterone released from the testes during development masculinizes and

defeminizes the brain and body.
• The ovaries do no release hormones during development, causing the default
feminized brain and body.
• Sexual dimorphisms are differences in the size or appearance of a structure between
males and females.
• Gonadal steroid hormones cause a sexual dimorphisms in the sexually dimorphic
nucleus of the preoptic area in male rodents.
• The interstitial nuclei of the anterior hypothalamus might be sexually dimorphic in
the human brain, however the differences in size are small and controversial.
• A critical period is a period of time in development where hormones have the most
effect in determining the structure and function of the brain and body.
• Organizational effects of gonadal steroid hormones occur early in life during
development and result in permanent changes in brain and behavior.
• Activational effects of gonadal steroid hormones occur later in life during adulthood
and result in temporary changes in brain and behavior that come and go as
hormones levels change.
• Androgen Insensitivity Syndrome is a condition in humans in which XY genotypic
individuals have dysfunctional androgen receptors.
• Congenital Adrenal Hyperplasia is a condition in humans in which XX genotypic
individuals have excess secretion of androgens during development.
• The Guevedoces of the Dominican Republic are XY genotypic individuals that have
deficient activity of the enzyme 5-α-reductase.
Attributions
Media Attributions
• Developmental Hormones © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• SDN-POA © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Organizational vs Activational © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• SDN-POA Testosterone © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Castration Effects © Casey Henley is licensed under a CC BY-NC-SA (Attribution
794 | SOCIAL BONDING
55.
SOCIAL BONDING
Long-term attachment, which includes pair bonding with

a sexual partner and parental bonding with offspring, are
naturally rewarding behaviors in some species of
Resources mammals.
Animal Model
• Glossary Terms
• Scientist links to learn Much of the research on social attachment and
more mammalian pair bonding has been done using voles as the
• Key takeaways animal model. Voles are useful because there are closely
related species that display considerably different
reproductive behavior. The prairie vole (Microtus
ochrogaster) that is indigenous to central North America
is an atypical rodent that displays highly social behavior, has monogamous relationships after mating,
and will cohabitate and share parental duties such as mutual resource collections, nest building and
care for their young. The montane vole, on the other hand, has a slightly different geographical
location and is a non-social species. They do not form pair bonds, and only the female cares for the
young.
SOCIAL BONDING | 795
Figure 55.1.
Prairie Voles.
Prairie voles are a
monogamous
species of vole
that forms
pair-bonds with
their mating
partners and
display biparental
care of young.
An experimental test called the partner preference paradigm can be used to assess vole monogamy.
Here, a three-chamber testing apparatus is used where a test vole is placed in a central chamber. In the
other two chambers are voles who have been harnessed into their rooms, unable to leave. The test vole
is then free to move between any of the three chambers. When a prairie vole has mated with one of
the harnessed voles, they will choose to spend more time with their “partner” vole compared to the
novel “stranger” vole, whereas a montane vole will choose to spend most of their time alone and spend
very little time with any other animal. Differences in brain and behavior can be studied between these
species to determine why they display these different reproductive behaviors.
Figure 55.2.
Partner
preference tests
can be conducted
to examine social
bonds in voles.
First, voles are
allowed to
cohabitate and
mate for 24
hours. Then, one
of the pair is
placed into a
preference
testing chamber
and is allowed to
spend time with
either the
partner animal or
a novel stranger.
In social voles like
the prairie vole,
mating will
induce a strong
preference for
the partner
animal over a
stranger. This
effect is not seen
in
non-monogamou
s voles like the
montane vole.
‘Vole Preference’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
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Not only do mated prairie voles prefer the company of their partner, they also demonstrate behaviors
similar to some human romantic relationships. For example, mated prairie voles living together display
selective aggression against a stranger, “intruder” voles, a behavior called “mate-guarding”. They also
spend significant time mutually caring for their young, their pair bonding can be modified by
psychoactive substance use, and they show increased anxiety when they are separated from their
partner vole.
Role of Oxytocin and Vasopressin in Pair Bonding

Comparing between monogamous and promiscuous species has allowed researchers to identify several
neurotransmitter signals that are implicated in pair bonding. Oxytocin and vasopressin seem to play
important roles in vole pair bonding, as inhibition of either of these signals decrease partner preference.
Additionally, differences in dopamine receptor levels and corticotropin releasing factors are observed
between vole species.
Hormone Control
The hypothalamus is a critical region for the formation of social bonds. Magnocellular
neurosecretory cells, the larger type of neurosecretory cell compared to parvocellular neurons, send
axons from the hypothalamus down to pituitary stalk where they terminate on capillaries of the general
circulation located within the posterior pituitary. Therefore, unlike the control of stress and gonadal
hormones, where the hypothalamic neurons release hormones onto anterior pituitary endocrine cells,
release of hormones from the posterior pituitary comes directly from hypothalamic neurons.
Figure 55.3. The

hypothalamic
magnocellular
neurosecretory
neurons release
oxytocin and/or
vasopressin into
the general
circulation via
capillaries located
in the posterior
pituitary.
‘Oxytocin and
Vasopressin
Release’ by Casey
Henley is
licensed under a
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The magnocellular neurons synthesize and release oxytocin and vasopressin, two neuropeptides, into
the blood. Oxytocin, often referred to as the love hormone, promotes social bonding. It is released
during reproduction and also causes uterine contractions during labor and the milk letdown reflex
after birth. Vasopressin, also called antidiuretic hormone, plays a role in regulating salt concentration
in the blood by acting on the kidneys to promote water retention and decrease urine production.
Further, it constricts blood vessels, raising blood pressure. Vasopressin has also been shown to be
involved in bonding, parenting, territoriality, and mate guarding in some animals. Biochemically,
vasopressin is very similar to oxytocin, as they are both nine amino acid peptides differing by only two
residues.
Figure 55.4.
Amino acid
sequences of
oxytocin and
vasopressin. The
amino acids that
are different
between the two
neuropeptides
are bolded.
‘Oxytocin and
Vasopressin’ by
Casey Henley is
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In the social prairie voles, oxytocin and vasopressin are released by the hypothalamus in response
to mating and act on regions of the reward and limbic systems. Specifically, there are increases in
oxytocin release in female prairies voles following mating. Female prairie voles express higher levels
of oxytocin receptors in the nucleus accumbens compared to montane voles. Oxytocin binding to
oxytocin receptors within the nucleus accumbens is necessary for females to form pair bonds and may
reinforce the association of reward with a partner. This is supported by experimental evidence that
when oxytocin is administered to females, there is an increase in pair bond formation. Female montane
voles have decreased oxytocin receptors within the nucleus accumbens, and thus do not form pair
bonds. Oxytocin does not have the same effects in male prairie voles.
Male prairie voles have an increase in vasopressin release following mating. The male prairie voles
express higher levels of vasopressin receptors in the ventral pallidum compared to montane voles. When
vasopressin is given to males it increases pair bond formation, and when a vasopressin antagonist is
administered prior to mating, pair bond formation is prevented. Vasopressin has no effect on female
prairie voles. The nucleus accumbens and ventral pallidum are both located in the basal ganglia and are
involved in the limbic loop, which is responsible for processing of emotions, rewards, and motivation.
An interesting experiment was done that overexpressed vasopressin receptors in the ventral pallidum
of male montane voles. The simple manipulation of this one protein completely the changed the
behavior of the monogamous montane voles such that they now behaved like prairie voles by forming
pair bonds and helping to take care of young.
Human bonding
Oxytocin, vasopressin, and the reward system also appear to be important for bonding in humans.
When presented with pictures of either their own children or partners, subjects in an fMRI show
increased activation in regions like the ventral tegmental area and striatum compared to when viewing
pictures of friends. These regions are also known to express oxytocin and vasopressin receptors, and
the hormones are released during times of bond formation, like breastfeeding and intercourse.
Love
Love can be thought of as an intensely strong attachment towards a person (romantic love, lust), a
thing (passion project), or concept (patriotism as a love for country, or altruism as a love for fellow
humans). However, it is very difficult to put a strict biological definition on these varied concepts of
love. This section focuses on interpersonal love, of which there are several unique forms, each resulting
in different behavioral outcomes. For example, romantic love drives physical attraction, lust, and sexual
activity. Parental love, on the other hand, encourages self-sacrifice and hyper-attentiveness towards a
newborn. Some behaviors are shared between the two forms of love, such as respect.
Romantic Love
Romantic love drives much of human behavior and has been documented thoroughly in the arts all
the way from Homer’s Iliad through Shakespeare’s Romeo and Juliet up to modern Taylor Swift.
The majority of human societies have embraced social monogamy, the romantic relationship
characterized by a pair of people who share resources, parenting duties, and exhibit preferential mating.
Outside of humans however, only 9% of mammalian species form socially monogamous pairs, while at
least 75% of bird species maintain socially monogamous relationships (which may explain the origin of
the phrase “love birds”).
Dr. Helen Fisher, an anthropologist and a leader in the field of love research, suggests that love can
be divided into three closely interconnected components. These three are guided in part by different
signaling pathways, and lead to somewhat different behavioral outcomes.
Lust (or libido) refers to a very strong desire for sexual gratification. These behaviors are largely
driven by the actions of the sex hormones testosterone, estradiol, and progesterone, released
downstream of activation of the HPG axis. Both testosterone and estradiol contribute to sex-seeking
behaviors in men and women, where increasing testosterone levels drive up sexual desire. The amygdala
also plays a significant role in mediating lust, and lesions may either result in hypersexuality (Kluver-
Bucy syndrome) or a decrease in responding to socially-derived sex cues.
Attraction is characterized by high energy investment and preoccupation towards a small number
of people. From an evolutionary perspective, attraction may have developed to discriminate between
multiple reproductive partners, allowing the focusing of limited resources towards fewer partners.
In Fisher’s theory, attraction is strongly related to the action of dopamine and norepinephrine. In
humans, the reward circuitry is involved in feelings of love. Using fMRI, Fisher presented pictures
of a patient’s romantic partner to them and identified increases in the blood flow to dopaminergic
midbrain areas such as the ventral tegmental area and the striatum. This finding compares with
imaging studies that observed increases in blood flow to insula, premotor, and hypothalamus as well
as striatum in response to highly erotic, sexual imagery. These studies suggest that romantic love and
lust have different driving neural structures underlying these behaviors. Norepinephrine functions to
increase attention, alertness, and energy, which accounts for the exhilarated feeling you may feel when
spending time with a potential partner.
Attachment is the long-term association with an individual accompanied by feelings of comfort
and emotional stability. Attachment also contributes to behaviors that maximize offspring
survivability, such as sharing parenthood responsibilities and protectiveness towards offspring. The
major neurochemical drivers of this form of love are oxytocin and vasopressin.
Parental Love
Parental love refers to instinctive affection towards one’s offspring. Parental love behaviors include
nurturing (collecting and sharing resources), protecting (promoting aggression against “intruders”),
and preparing one’s young for their adult life (risk assessment training). In evolutionary theory,
parental love serves to improve the odds of passing of one’s genes through the following generation.
Most large mammals (humans included) are K-selected species, which benefit most from a small
number of high-quality offspring that require substantial parental investment, as opposed to large
numbers of offspring with little investment.
An extension of parental love is familial love or kinship, the protection and preferential support
of one’s extended genetic relations. Because blood relatives share common genes, increasing the
survivability of these family members has benefits for passing genes to the next generation. In the words
of the geneticist Jack Haldane, “I’d gladly give my life for two brothers or eight cousins.”
Many behaviors related to mammalian motherhood are accompanied by changes in neural activity.
Nursing, for instance, is feeding behavior that is regulated through a positive feedback cycle. Offspring
suckling activates the mother’s somatosensory afferents. Through a series of oxytocin-dependent
circuits across the hypothalamus, suckling ultimately increases lactation through the milk letdown
reflex. Auditory sensory inputs such as the sounds of a crying baby can also trigger this reflex.
Sometimes, just thinking about the baby can induce letdown. Some changes are dependent on neural
plasticity. For example, after childbirth, the auditory areas of rodents rewire to become more sensitive
to high frequency sounds. This adaptation allows the mothers to better detect the ultrasonic
vocalizations that are emitted by offspring when they are distressed or hungry. Olfactory areas also
change in order to become more sensitive to the particular odorants given off by their young, allowing
them to better identify their offspring. In humans, these olfactory changes result in decreased aversion
towards traditionally aversive stimuli (urine or fecal matter) when they originate from their children.
Key Takeaways
• Prairie voles are a socially monogamous species that forms pair bonds with their
mating partners and display biparental care of young.
• Montane voles are a closely related species that display a promiscuous mating
strategy, do not form pair bonds, and do not show biparental care of young.
• Female prairie voles have increased oxytocin receptors within the nucleus accumbens
compared to montane voles.
• Male prairie voles have increased vasopressin receptors within the ventral pallidum
compared to montane voles.
• Oxytocin and vasopressin are both neuropeptides released from the posterior
pituitary.
• Human bonding and love (romantic and parental) has also been shown to involve
oxytocin, vasopressin, and reward circuitry.
Attributions
Media Attributions
• Prairie Vole © Davi adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• VolePreference © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• PosteriorPituitary © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• OxytocinVasopressin © Casey Henley is licensed under a CC BY-NC-SA (Attribution
804 | SLEEP AND EEG
56.
SLEEP AND EEG
Sleep is such an important part of our lives that a lack of it

strongly correlates with negative outcomes on nearly every
measure of health. People who sleep less than
Resources approximately 7 hours a night are at a greater risk for heart
disease, stroke, asthma, arthritis, depression, and diabetes.
Nearly 20% of all car crashes, both fatal and nonfatal, are
attributed to drowsy driving. The cancer research branch
• Glossary terms of the World Health Organization has determined that
• Key Takeaways disruption of regular sleep is “probably carcinogenic to
humans,” putting it in the same risk category as the
infectious agents malaria and human papillomavirus
(HPV), as well as the biochemical weapon mustard gas.
Sexual health is affected by sleep deprivation as well, as men with the worst sleeping habits have
significantly lower sperm counts, decreased circulating testosterone, and even testicular shrinkage.
Despite all that we know about the benefits of sleep, sleep is often the first time commitment to get
cut, often getting squeezed as people stay awake later while waking up sooner. Consider that the CDC
estimates that more than a third of American adults fail to get enough sleep each night. Almost 70% of
college students fail to get the recommended amount of nightly sleep, and half of all college students
report experiencing daytime sleepiness as a result.
The current medical recommendation is 7-9 hours of sleep each night. But why is sleep so
important? It is possible to study sleep using a combination of techniques; the output of sleep studies
are visualized on a polysomnogram (pahle-SOM-nuh-gram; somn- is the prefix referring to sleep).
Several physiological measures are taken in a polysomnogram, including heart rate, blood pressure and
oxygenation level, respiratory depth and pattern, muscle activity, eye movement, and one of major
interest to neuroscientists, brain wave activity. While everyone knows what sleep is, it is useful to try to
more precisely define sleep as a biological function.
SLEEP AND EEG | 805
Sleep is characterized by a decrease in physical activity, a decoupling from external inputs, and
changes in brain wave activity.
Compared to waking behavior, a person’s physical activity is greatly decreased when they sleep.
While asleep, people are relatively inactive, and as a result the body uses about 10% less energy. This
is not to say that people do not completely cease all movement during sleep. It is very common to
readjust posture many times in the middle of the night. Some people may grind their teeth together or
talk in their sleep, sometimes carrying on full conversations by themselves! About 15% of people have
experienced somnambulism, or sleepwalking: full on wake-like behaviors such as navigating down a
flight of stairs or preparing a sandwich, performed entirely in the absence of intent of memory recall.
Despite these rare occurrences of physical activity, the average movement of the person over a night’s
rest is still less than their average activity when awake.
When we sleep, our conscious brains are “distanced” from the outside world. Sleep causes a
heightened threshold for detection of stimuli, so we do not receive the same magnitude of inputs from
our sensory systems as when we’re awake. This is why someone else might have to talk loudly or even
shake you physically to wake you up.
Lastly, sleep was once thought to be a period of time characterized by low brain activity. After all,
the person looks like they are not moving. Shouldn’t brain activity be reflective of that decreased state
of activity? With the advancement of EEG technology in 1924, and the rise of sleep laboratories in the
1970s, scientists who studied brain activity noticed that, at different times throughout the night, the
brain of a sleeping person was very similar in activity to the brain of an awake person!
EEG
Electroencephalography (EEG) is used to measure brain rhythms through recording electrical
activity at the scalp, making it a non-invasive and painless tool to detect brain activity. EEG detects the
general activity of the cortex by measuring small (~10 uV) fluctuations in voltage. This technique does
not measure electrical activity of a single neuron, but rather measures the electrical activity of a large
population of neurons, on the order of thousands of cells, in order to generate a signal that is large
enough to detect.
An EEG reading depicts differences in both amplitude and frequency over time. An increase in
amplitude on an EEG indicates a higher degree of synchronous activity of cortical neurons, whereas
the frequency of an EEG indicates how often neural synchrony occurs. EEG is used to measure
806 | SLEEP AND EEG
patterns of brain activity during different behavioral states and is used in both research and in the
diagnosis of various neurological conditions such as sleep disorders and epilepsy.
Figure 56.1. EEG

recording setup.
In an EEG,
electrodes are
placed on the
head that can
detect and record
neuronal activity
of the cortex.
How is synchronous activity generated?

Though EEG is used to measure brain rhythms, the function of these rhythms is unknown, but they
have been correlated to different behavioral states such as wakefulness, sleep, attentiveness, and various
pathologies. Brain rhythms generated through synchronous neuron activity can be created through
two different mechanisms.
1. Cells within the thalamus have been demonstrated to have pacemaker properties that allow it to
generate rhythmical cortical activity.
2. Collections of cells can also generate intrinsic rhythms through their collective activity.
EEG patterns during sleep

Each night when we go to sleep, our brains undergo a very stereotyped pattern of activity changes.
At times, neurons in the cortex exhibit synchronized patterns of firing. And at other times, cortical
activity looks very similar to an awake brain. We can divide sleep roughly into two different phases
depending on one of the first physiological measures that sleep scientists studied: eye movement. A
study published in 1953 used a device to detect eye movement while a person was asleep. Interestingly,
they noticed that at some points in the night, usually first occurring around three hours after falling
asleep, the patient’s eyes would dart rapidly and jerkily back and forth, a pattern of activity that the
University of Chicago researchers called rapid eye movement (REM). This first period of REM
activity lasted for about 20 minutes, after which the eyes would stop moving again. This activity
SLEEP AND EEG | 807
pattern repeated every hour or two for the rest of the night. They used eye movement to separate sleep
into two phases: REM sleep, and nonREM sleep (NREM sleep.)
In addition to eye movement, they observed and measured other physiological behaviors.
Respiration rate and heart rate both increased during the REM phase of sleep and dipped during
NREM sleep. They also (rudely) woke up patients throughout the two phases of sleep and found that
patients were more likely to recall dreams with visual imagery if their REM sleep was interrupted.
Those woken during NREM sleep were less likely to recall dreams, hinting that dreaming is more likely
to happen during REM sleep. While eye movement could differentiate between two phases of sleep,
EEG could further subdivide NREM sleep. Using EEG, scientists discovered three distinct NREM
phases based on neuron activity patterns: NREM1, NREM2, and NREM3 sleep.
Currently, readings collected via EEG are considered to be the gold standard for measuring the stages
of sleep. But before we describe EEG traces while asleep, we should describe the EEG of a person who
is awake. Usually, the awake EEG is dominated by high-frequency waves, that increases with attention
and mental activity.
NREM1 is the earliest stage of sleep. It’s also described as relaxed wakefulness, drowsiness, or light
sleep. During NREM1, mostly alpha waves are present and a person’s muscles are still somewhat
active. Their eyelids may open and close every so often, and they may still respond to questions.
Basically, the deeper into NREM1 sleep a person becomes, more waves with lower and lower
frequencies start to emerge. NREM1 transitions into NREM 2, which mostly has theta waves.
Lastly, NREM3 occurs, which is also called deep sleep. At this phase of the night, a person’s
physiological activity drops to its lowest point of the night: heart rate, respiration, blood pressure, and
metabolism all reach minimum during NREM3. In this stage of sleep, many of the cortical neurons
fire in synchronicity with one another, and the subsequent change in potentials cause large amplitude
deflections in the EEG, called delta waves.
The EEG trace of a person in REM sleep is quite the opposite of what is seen in deep sleep. Instead
of large amplitude events at a low frequency, the REM brain has a lot of low amplitude events at a high
frequency. In fact, the brain in REM sleep has a pattern of activity that is more similar to a person who
is awake than asleep! Because of this asynchronous firing activity, REM sleep is sometimes also called
paradoxical sleep.
808 | SLEEP AND EEG
Figure 56.2. EEG

activity during
different stages
of sleep.
Brainwave
activity changes
drastically over
the course of the
different stages
of sleep. REM
sleep brain
activity most
closely resembles
brain activity
while awake.
Progression through the stages of sleep

To illustrate the stages of sleep that a person experiences each night, we can use a hypnogram. These
charts to plot time on the x-axis, and stage of sleep on the y-axis. Awake is represented at the top,
and deep sleep is at the bottom. For an average night’s rest, neural activity will fluctuate through the
four phases relatively predictably. When a person first falls asleep, they will move from NREM1 down
through NREM2 then NREM3, before coming back out of deep sleep progressively back to NREM1.
After NREM1, they may enter REM sleep before transitioning back through the stages to NREM3
again. This cycle of activity repeats roughly every one and a half hours. People spend a larger percentage
of each cycle in deep sleep and very little time in REM sleep early in the night. On the other hand, in
the last few cycles before waking up from a full night’s rest, people spend a larger percentage of each
cycle in REM sleep, and almost no time in deep sleep.
SLEEP AND EEG | 809
Figure 56.3.
Hypnogram
showing
progression of
sleep stages
through the
night. After
falling asleep, an
individual starts
in REM sleep,
and then
progresses
through Stage 1,
Stage 2, and
Stage 3 of NREM
sleep. They then
progressively
return to REM
sleep. As the
night progresses,
an individual
spends less time
in deep sleep and
more time in
REM sleep.
Why do we sleep?
All organisms that we know of experience some type of sleep. But we still haven’t figured out exactly
why animals sleep. Here, we will discuss three theories that have been proposed to explain sleep.
None of these theories alone fully explains the complex phenomenon of sleep, and they are not
mutually exclusive. The most likely reason we sleep is probably some combination of the following
three theories.
Recuperation Theory
The recuperation theory of sleep is centered around the idea that being awake is stressful and exerts
a physically demanding toll on the body. The body therefore needs a period of time when energy
810 | SLEEP AND EEG
usage decreases and the body’s natural repair systems can work without disruption. Sleep is how the
body “wipes the slate clean” and resets. Evidence for the recuperation theory comes from experiments
tailored around the idea of looking at what happens when a person doesn’t get sufficient sleep.
As anyone who has ever pulled an all-nighter can attest, a single night of sleep deprivation often leads
to significant psychological changes, including anxiety, irritability, and mood swings. Staying awake
even longer than 24 hours can cause more severe changes in mind state, such as temporary psychosis,
hallucinations, or delusions.
While the support for the recuperation theory is generally true for almost all people, there are about
1% of people who seemingly gain the restorative benefits of sleep, even with fewer than 6 hours of
sleep each night. They may wake up at 4:30 in the morning feeling completely refreshed. And yet,
despite getting so little sleep, these short sleepers have similar health outcomes with respect to body
mass index and psychiatric measures such as depression and overall optimism. Something about the
circadian rhythms of these short sleepers allows them to “maximize” their sleep efficiency. Many have
very short sleep latencies, meaning they fall asleep within minutes after lying down – as quickly as
someone with narcolepsy. They also spend a larger percentage of their night in deep sleep and REM
sleep while minimizing NREM1 and NREM2.
Evolutionary Adaptation Theory

The evolutionary adaptation theory is the idea that animal sleep patterns are different across species
for reasons that most efficiently benefit each animal. Over millions of years of evolution, individuals
with the most ideal sleep patterns have an advantage, and their sleep habits will be selected for in the
following generation. For example, consider humans. As an animal highly dependent on light and the
visual system for navigation and accurate performance of tasks, the dark is a very dangerous time to
be active. The risks of wandering off a cliff, running head-first into a tree while escaping a predator, or
eating a wrong-colored poisonous berry would all be elevated in the dark. We benefit from behaviors
that minimize those risks, such as inactivity until the sun rises. During this inactive period, sleeping
decreases our metabolism and our body’s need for energy.
Humans are just one animal that has an evolutionarily fine-tuned sleep pattern. If you look across
the animal kingdom, you’ll find all varieties of sleep behaviors that are best fitted to the needs of the
individual species. In the wild, for example, dolphins are generally prey. They evolved with the ability
to put one half of their brain to “sleep” at a time, allowing the “awake” half to keep an eye out for
potential predators. Small prey animals, like squirrels, are faced with the threat of being attacked at
SLEEP AND EEG | 811
night. For them, remaining very still, quiet, and hidden improves their survival. Tigers, the top alpha
predators in any ecological niche, have almost no predators to hide from, allowing them the luxury of
sleeping up to 20 hours a day.
This evolutionary adaptation theory argument has a major weakness, however. In almost all animals,
sleep represents a period of time when an organism’s ability to use their sensory organs to detect the
hallmark signs of an approaching predator, like the flurry of feathers from a hungry owl or the soft
padding of a wolf footsteps, decreases drastically. For an animal that can’t hide very effectively, sleep
represents a period of vulnerability, as they would be unable to sense incoming threats.
Brain Plasticity Theory

The brain plasticity theory suggests that the brain needs some period of time for critical changes
to occur. During sleep, circuits in the brain undergo consolidation processes that are important for
memory formation. For example, academic performance and examination grades worsen as a person’s
nightly sleep decreases. Both the REM and NREM3 phases of sleep are important for different types
of memories, and studies suggest that declarative memory, pieces of information about facts, benefits
more from slow-wave sleep while procedural memory, the learning of motor skills, is enhanced by
REM sleep.
Although the exact mechanisms about how sleep improves memory are unknown, we theorize that
brain activity during sleep helps move memories held in “temporary” areas into areas of stable, long-
term storage. The evidence in support of this theory starts with looking at the brains of newborns.
When you were first born, during those first few weeks of life, you slept close to nearly 70% of the day,
almost 17 hours! At this point in your life, your brain starts to experience all sorts of new sensations:
your eyes detect visible light for the first time, the skin feels the air blow past it, and the ears sense
new frequencies and combinations of sound waves. As a result of these stimuli, scientists hypothesize
that your brain undergoes as many learning events as rapidly as possible. This rapid learning helps you
remember what you learned each day so you can respond to your environment as you grow up.
812 | SLEEP AND EEG
Sleep Disorders
Insomnia
Almost everyone has experienced difficulty sleeping at some point in their lives, often as a result of
stress or anxiety. For example, it might be difficult to fall asleep the night before a big interview,
or you may wake periodically in the hours before an important early morning flight. There is no
strict definition for insomnia. The major clinical symptoms are self-reported measures, such as a
dissatisfaction with nightly sleep or a change in daytime behavior, such as sleepiness, difficulty
concentrating, or altered mood states. The lack of clear diagnostic criteria makes estimating prevalence
difficult, but some guesses put the number of people with insomnia close to one third of the US
population. Common triggers for insomnia include heightened anxiety, stress, or advanced age. It may
also be downstream of other diseases, such as Parkinson’s disease, diabetes, depression, or chronic pain
conditions. Lifestyle can also be a major risk factor for insomnia, as jet lag and working late-night shifts
can disrupt sleep patterns.
We can describe insomnia as acting at two stages. Onset insomnia is defined as a difficulty with
initially falling asleep. People with onset insomnia will frequently lie in bed for a long time before
finally drifting off. Maintenance insomnia, however, is a difficulty with remaining asleep. People with
maintenance insomnia experience many waking events throughout the middle of the night, or they
may wake up very early in the morning and be unable to get back to sleep. The two are not exclusive,
and people may experience both forms of insomnia in a single night.
The most effective treatments for insomnia begin at the level of behavioral changes. Improving
sleep habits, such as minimizing arousal states before bedtime, developing a reliable pattern of sleep-
wake timing, eliminating caffeine intake in the afternoon and evening, and increasing daytime physical
activity can decrease insomnia. Prescription medications are less preferred for insomnia treatment,
since these drugs are more effective at inducing unconsciousness rather than biological sleep. These
drugs can also have adverse psychological side effects such as mood swings and depression, and those
adverse effects may be more severe than insomnia itself. Prolonged use of prescription sleep
medications can lead to a “rebound effect,” causing a person to experience even worse insomnia when
they are unable to get sleep drugs. This is called iatrogenic insomnia, and can lead to a cycle of
dependence.
SLEEP AND EEG | 813
Narcolepsy
Unlike the previous two sleep disorders, which result in a deficit of sleep, narcolepsy can be thought of
as an “excess” of sleep. More accurately, narcolepsy is inappropriate sleep, and it manifests as frequent
sleep attacks throughout the day, each event lasting for seconds or minutes at a time. An estimated 1
in 2000 people experience narcolepsy. One of the life-threatening symptoms that appears in narcolepsy
is cataplexy, which is the sudden weakening of muscle tone that accompanies a sleep attack. A
cataplectic attack may cause someone to physically fall over during a narcoleptic incident. Cataplexy
often happens during high emotional states, such as excitement. As with other sleep disorders, changes
in lifestyle can improve the course of narcolepsy. Introducing short daily naps can be helpful, as can
general good sleep habits (minimal digital device usage before sleep, regular sleep-wake timing, and
physical activity). Drugs such as amphetamines (Modafinil) can be used in the daytime to stimulate
activity in the CNS and can be prescribed to treat severe cases of narcolepsy. Some antidepressant drugs
can be used to treat cataplexy.
The exact cause of narcolepsy has not yet been identified. However, there are many clues that point
to a dysregulation of the signaling molecule orexin produced by cells in the lateral hypothalamus. These
neurons die off in people with narcolepsy, but the cause of why the neurons die is unknown. Also,
having a genetic predisposition to narcolepsy does not guarantee that a person will experience the
symptoms, indicating that there is some combination of genetic and environmental factors that lead to
narcolepsy.
Fatal familial insomnia

While many cases of sleeplessness last a day or two, and some cases are clinically significant and treatable
with behavioral changes, a very small fraction of cases of insomnia are incurable and deadly. In people
with fatal familial insomnia (FFI), they experience severe insomnia. Some patients stay awake for up
to six months at a time. As a result of either the disease or the sleep deprivation, they experience altered
mood states, hallucinations, dementia, and eventually death, usually within two years after a diagnosis
is made. The cause of FFI is unknown. There is a strong genetic component associated with it, as it
appears frequently within certain family trees. But, there have also been a few cases of sporadic FFI,
people with no apparent family members with the disease. One observation in common among people
with FFI is significant damage to the thalamus, as a result of misshapen proteins called prions, a similar
disease-causing agent that is responsible for mad cow disease.
814 | SLEEP AND EEG
REM sleep behavior disorder

One very rare sleep disorder called REM sleep behavior disorder (RBD) can cause people to carry
out complex, highly coordinated motor actions while they are sleeping, sometimes acting out their
dreams as if they were reality. People with RBD are at risk of injuring themselves or others. Their sleep
actions may be in response to a violent nightmare, causing them to jump out of bed, kick or punch
the air, run through the house, or throw things. One of the most shocking instances of parasomnia-
induced sleepwalking was the 1987 case of Kenneth James Parks, a Canadian man who, in his sleep,
drove 23 km to the house of his in-laws and stabbed both of them with a kitchen knife. After his arrest,
scientists discovered that his brain activity was highly abnormal during sleep. As a result of the medical
examination, the Supreme Court of Canada acquitted him of murder in 1992.
Key Takeaways
• EEG is used to measure brain activity through the scalp and is used to measure brain
activity during sleep.
• Sleep can be divided into REM and NREM sleep (NREM1, NREM2, NREM3).
• REM sleep EEG readings most closely resemble awake EEG readings.
• The progression of the stages of sleep starts in REM sleep, then progresses through
NREM 1, NREM2, and NREM3 before cycling back to REM sleep. As the night
progresses, an individual spends more time in REM sleep and less time in deep sleep.
• The reason why we sleep is not fully understood but is likely a combination of three
theories: recuperation theory, evolutionary adaption theory, brain plasticity theory.
• There are disorders of sleep that can cause either lack of sleep or excessive sleep.
SLEEP AND EEG | 815
Attributions
Media Attributions
• EEG recording © Petter Kallioinen adapted by Valerie Hedges is licensed under a CC BY-SA
• Stages of sleep © Cheryll Fitzpatrick and Christina Thorpe adapted by Valerie Hedges is licensed
under a CC BY-SA (Attribution ShareAlike) license
• Progression through stages of sleep © Cheryll Fitzpatrick and Christina Thorpe adapted by
Valerie Hedges is licensed under a CC BY-SA (Attribution ShareAlike) license
816 | CIRCADIAN RHYTHMS
57.
CIRCADIAN RHYTHMS
Almost every living organism that we know of on Earth

exhibits some sort of cyclic pattern of activity that closely
matches the rising and setting of the sun.
Resources
Retinohypothalamic Tract
• Glossary terms Our sleep and wake cycles are associated with the day/
• Scientist links to learn night cycle. Previously, we discussed the retinal ganglion
more cells that transmit signals from the rods and cones of the
• Key takeaways retina into the brain for processing within the lateral
geniculate nucleus and primary visual cortex. A separate
population of retinal ganglion cells do not process signals
from the rods and cones, but rather serve as
photoreceptors and are directly sensitive to light, communicating whether or not it is daytime.
These cells send their axonal projections separately from the optic nerve that sends most visual
information. Rather, they project through a pathway called the retinohypothalamic tract, synapsing
on a clump of cells in the hypothalamus called the suprachiasmatic nucleus (SCN). In turn, these
cells of the SCN have projections to a gland in the brain called the pineal gland, so named for
its pinecone-like shape. The pineal gland converts the amino acid tryptophan into melatonin, an
endogenous hormone, which is then secreted into the bloodstream. Melatonin helps the brain regulate
the sleep-wake cycle with increased melatonin levels helping to signal the body to prepare for sleep.
The production of melatonin is heavily dependent on exposure to sunlight. In the daytime, the
SCN tonically inhibits activity of the pineal gland, resulting in low production of melatonin. But
when daylight starts to decrease, the retinohypothalamic tract sends a weaker excitatory signal onto the
CIRCADIAN RHYTHMS | 817
SCN, which allows increased pineal gland activity. Light exposure is the main environmental influence
that decreases melatonin levels.
Figure 57.1. Pineal

gland and
melatonin. A
group of retinal
ganglion cells
within the retina
are
photosensitive
and are able to
communicate
day/night
lighting
conditions
through the
retinohypothala
mic tract. The
photosensitive
retinal ganglion
cells synapse on
the
suprachiasmatic
nucleus. The
pineal gland
produces the
neurohormone
melatonin. The
suprachiasmatic
nucleus inhibits
activity of the
pineal gland and
production of
melatonin. Under
nighttime
lighting
conditions, there
is weaker
activation of the
retinhypothalami
c tract, and thus
decreased
inhibition of the
pineal gland,
leading to
increased
melatonin
production.
‘Pineal gland and
melatonin’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Not all wavelengths of light are equally potent at dampening melatonin production. The shorter
wavelengths of light, down in the violet-blue range, are much more efficient at activating the
retinohypothalamic tract compared to longer, yellow-red wavelengths. Increased retinohypothalamic
tract activation leads to decreased melatonin production, which can delay the onset of sleep.
Fluorescent or LED lighting and digital devices, such as computer screens and cell phone screens,
use blue wavelengths of light. Therefore, the best advice to optimize your sleep habits is to eliminate
exposure to all digital devices about one hour before your intended bedtime.
Circadian Rhythms
The discovery of 24-hour patterns of behaviors began in 1729 with the French scientist Jean-Jacque
d’Ortous de Mairan, who documented the movement of the Mimosa pudica plant. This unique
organism was chosen since the plant exhibits heliotropism, light-seeking movements. In particular,
this plant opened up the leaves in the daytime to capture sunlight, then closed at night, minimizing
predation. When the plants were put into a dark room with no exposure to sunlight, to his surprise
they still opened and closed their leaves in time with the clock. Mairan concluded that the plant did
not change its behavior in response to light, but rather in response to some internal 24-hour clock.
His work laid the foundations for future chronobiologists, scientists who study day-night dependent
periodic phenomena in living beings.
Figure 57.2. The

Mimosa pudica
plant displays
light-seeking
movements, with
the leaves of the
plant opening
during daylight
and the leaves
closing at night.
During an
experiment in
which the plants
were put in a
dark
environment for
24 hours,
surprisingly, the
plant still opened
and closed its
leaves on a
24-hour cycle,
demonstrating
that the plant
had an internal
clock controlling
the opening and
closing of leaves.
Any behavior or physiological measure that intrinsically cycles on a 24-hour pattern is said to be a
circadian rhythm. The word circadian comes from the Latin words ‘circa’ meaning “around”, and
‘diem’ meaning “day”. Compare this with an ultradian rhythm, any cycle that is faster than 24 hours,
such as the cycling between deep sleep and REM sleep every 90 minutes. Alternatively, compare with
infradian rhythms, patterns that are longer than 24 hours, like the 4-week-long human menstrual
cycle.
Although we mostly think of the circadian rhythm in the context of sleep and wake, many other
physiological measures fluctuate reliably throughout the day. Body temperature dips late in the
evening, putting the body into a low-energy state that helps promote sleep. Withdrawal reflexes peak
around midnight, hunger-driving hormone production rises before lunch and dinner, attention is
usually highest in the morning – all manner of behaviors that rise and fall depending on the time of
day can be said to be a part of a circadian rhythm.
Figure 57.3.
Circadian
rhythms in the
body.
Throughout a
24-hr period of
time, there are
many behavioral
and physiological
changes that
occur through
the day.
Circadian Rhythms are Entrainable

Luckily, a person’s circadian rhythm is not permanent. Anyone who has traveled overseas to a different
time zone for more than a few days has experienced that uncomfortable sensation called jet lag, where
a person experiences psychological symptoms such as difficulty concentrating and mood swings, and
physical symptoms like daytime fatigue, insomnia, and gastrointestinal distress (nausea, constipation,
or diarrhea). Jet lag happens when there’s a mismatch between the internal environment and the
signals that the brain receives from the outside world.
If you flew east from Chicago to Cairo, for example, your circadian clock will be off by 7 hours.
When your internal “Chicago” clock is telling you to start getting sleepy around 11 PM, the sun will
be rising in Cairo, as the locals are starting to wake up. You may be eating when you’re not hungry or
laying down in bed when you’re not sleepy, and this mismatch contributes to jet lag. However, with a
few days of adjustment, you will be able to overcome jet lag. You will start sleeping as the sun sets, you
will get hungry at the same time as the Egyptians, and your physiological measures will start to align
with your time zone.
This adjustment is only possible because our circadian rhythms are entrainable, meaning they are
able to change and fit the surroundings. Our circadian rhythms entrain in response to zeitgebers, the
German word for “time givers”: environmental cues, such as increased light exposure when the sun
comes up, or social cues, such as increased sensory input from heightened activity of the people around
you. A rise in the neurohormone melatonin is an important signal that contributes to helping the
brain entrain, which is why taking a melatonin supplement late at night when in a new time zone may
help someone get over jet lag more quickly.
Figure 57.4.
Free-running and
entrained
circadian
rhythms. In the
absence of
zeitgebers,
circadian
rhythms will free
run. Typically,
this means that
the 24-hour cycle
will shift 2 hours
later each day.
When the cycle
is entrained
again to a 24
hour rhythm, the
circadian rhythm
will no longer
free run and will
be consistent.
‘Free-running
and entrained
circadian
rhythms’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
If sunlight is a trigger that helps a person entrain their circadian rhythm to new environments, what
would happen if a person is completely isolated from sunlight? In other words, what does a free-
running circadian rhythm look like? One of the early documented case studies addressing this
curiosity was conducted by a French cave explorer named Michel Siffre. In 1972, Siffre (voluntarily)
spent six months deep in a Texan cave to evaluate what would happen to a person completely isolated
from zeitgebers. At the end of the experiment, he found his circadian cycle was much longer than 24
hours, and very unpredictable – some of his so-called days would consist of being awake for 36 hours
and asleep for 12.
A more rigorous scientific study, conducted in a group of people living in an underground bunker
with unchanging lighting conditions for several days estimates the typical free-running circadian cycle
to be close to 26 hours. In other words, a person absent from external cues begin to fall asleep and
awaken 2 hours later each day. Free-running circadian disruption is a potential issue for scientists
aboard the International Space Station, who experience about 16 sunsets and sunrises per day, since
the orbiting space vessel completes one trip around the earth every 90 minutes. In order to minimize
the negative effects of jet lag on the researchers aboard, NASA has the inside of the vessel set to an
artificial 24-hour cycle. Bright blue LEDs illuminate the cockpit in the “daytime,” while dim, red-
shifted wavelengths are used in the evening to induce sleepiness.
Key Takeaways
• The retinohypothalamic tract signals lighting conditions to the suprachiasmatic

nucleus.
• Activity in the suprachiasmatic nucleus inhibits the pineal gland and production of
melatonin.
• A 24-hour cycle is called a circadian rhythm
• Circadian rhythms are entrainable to zeitgebers, or external cues.
• Without zeitgebers circadian rhythms free-run, and will shift.
Attributions
Media Attributions
• Pineal gland and melatonin © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Circadian plant © Abubiju adapted by Valerie Hedges is licensed under a Public Domain license
• circadian rhythms © vaxzine is licensed under a CC BY-ND (Attribution NoDerivatives) license
• Free running rhythm © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
EMOTIONS: OVERVIEW | 825
58.
EMOTIONS: OVERVIEW
Emotions
At the airport, you can observe people experiencing a wide
Resources
range of emotions: Sadness at seeing family members off,
fear and anxiety for those about to fly for the first time,
love when a long-distance relationship is reunited, and
anger over unpredictable cancellations. Emotions are
• Glossary terms
complex neurophysiological states that contribute to an
internal feeling and guide behavior. Some emotions are
more
pleasant (joy), some are negative (disgust), and some are a
• Key takeaways
mix of both (nostalgia). Some are short-lasting (surprise)
while others may persist over years (vengefulness).
However, beyond this statement, it becomes very difficult
to put a clear-cut definition on “emotion”. The difficulty with defining emotion arises because of the
fluid nature of emotions: they exist on a spectrum, multiple emotions are experienced simultaneously,
each emotion is perceived by different people in unique ways, and everyone has a slightly different
interpretation and understanding of an emotion.
The field of affective neuroscience seeks to understand the neural mechanisms that underlie
emotion. The field has expanded with the help of functional imaging methods like EEG and fMRI,
where changes in brain activity can be measured and quantified as a person experiences different
emotion-provoking stimuli. Affective neuroscientists work to develop biology supported therapies
for disorders such as depression, PTSD, and addiction, which are dysregulations of the emotions of
sadness, fear, and desire, respectively.
826 | EMOTIONS: OVERVIEW
Faces of Emotion
Although best known for his theory on evolution, naturalist Charles Darwin published prolifically
about other topics in biology, ranging from botany, coral reefs, and even a treatise on psychology. In
this 1872 text, called The Expression of Emotions in Man and Animals, Darwin suggested that similar
emotional responding is found across different cultures, and to some extent, even in nonhumans. In
his view, the main purpose of emotive expression is to communicate survival cues between individuals:
a relaxed expression conveys safety, while a fearful expression promotes alertness, since danger may
be nearby. Darwin also suggested that we gain survival information from non-human behaviors, for
example, a hissing snake or a snarling lion is an immediate threat that should cause fear or other
avoidance behaviors.
Figure 58.1.
Displays of
emotion. Charles
Darwin published
images of
displays of
emotions in both
animals and
people in his The
Expression of
Emotions in Man
and Animals,
1872.
Moving forward into more modern times, the American psychologist Paul Ekman expanded on
Darwin’s theory, distilling down the range of human emotions to belonging in one of seven basic
categories of emotions: Anger, contempt, disgust, fear, happiness, sadness, and surprise. If the purpose
of emotion was to communicate pro-survival cues, then Ekman theorized that all humans, regardless
of culture, would use similar facial expressions.
To test this hypothesis, Ekman visited a remote village in Papua New Guinea, where he studied a
population that is isolated from any other known cultures. As predicted, these people made the same
facial responses in reaction to various emotional circumstances. In 1972, Ekman published his theory
of universal facial expressions.
Part of Ekman’s research led their team to develop a series of photographs of actors portraying six
major emotions. This Ekman 60 faces (EK-60F) test has since been used to assess facial emotional
with fascinating results: People with major depressive disorder or borderline personality disorder have
a lessened ability to detect happiness in others, seeing emotional faces results in a similar emotion in
the viewer, and people with dementia or Parkinson’s disease identify emotions as being less intense.
Ekman’s research led them to develop the Facial Action Coding System (FACS), a system that uses
facial anatomy to differentiate the features that are characteristic of different expressions. For example,
a feature of a happy face is the flexing of the zygomaticus major and orbicularis oculi muscles, which
produces an upward turn of the corners of the mouth and a rising of the cheeks. Other facial features,
such as head movement, eye movement, and larger physical movements are also scored, and are also
used to help identify emotions. The FACS can be used to formally describe why some smiles appear
as genuine (a Duchenne smile, with simultaneous muscle action) while others look fake or forced (a
non-Duchenne smile, characterized by the turn of the corners of the mouth without much change to
the top part of the face).
History of Emotion Research

One of the older theories about the origin of emotion is based on the most common sense
interpretation of cause and effect. For example, imagine some noticeable emotional stimulus, such as
encountering a hungry lion on the sidewalk. The logical cause and effect explanation suggests that
seeing the lion prompts the emotion of fear, which then causes the sympathetic nervous system
“fight-or-flight” response (elevated heart rate and blood pressure, increased respiration, and cellular
mobilization of energy). In the 1880s, psychologist William James and physician Carl Lange
independently developed a new theory about the origin of emotion. According to the James-Lange
theory of emotion, and contrary to a common sense understanding of the origin of emotion, the
body’s physiological changes precede the onset of an emotional response. For example, imagine
encountering that same hungry lion on the sidewalk. The James-Lange theory tells us that the
perception of the threat of being eaten causes the sympathetic nervous system response, and that these
physiological changes trigger the onset of fear.
Soon after, in the 1920s and 1930s, two physiologists named Walter Cannon and his doctoral
student Philip Bard criticized the James Lange theory. In one experiment, they surgically removed the
entire sympathetic nervous system from cats, destroying the nerves that regulate vascular dilation, the
activity of liver enzymes, and the reaction that causes the hair standing on end. These cats were then
put before a threatening aggressor. If the James-Lange theory was true, then the physiological changes
should precede the emotive response. However, the cats exhibited the fear / aggression response (such
as posturing, hissing, and clawing) even without an intact sympathetic nervous system. Relatedly,
patients with spinal cord injuries have a similar lack of autonomic inputs to the brain, but their
capacity for emotional responding is still intact.
In a second criticism, Cannon and Bard proposed that the physiological changes seen in sympathetic
nervous system activity may arise for a variety of reasons, not always for emotionally salient reasons. For
example, intense exercise causes strong cardiorespiratory changes; however, we do not necessarily feel
a strong emotional state after this physiological perturbation. Likewise, exogenous administration of
epinephrine, onset of fever, or being in cold temperatures may also trigger some physiological changes
without causing a strong emotional response. Based on their evidence opposing the James-Lange
theory, Cannon and Bard developed an alternative explanation for the origin of emotions. According
to the Cannon-Bard theory of emotion, the perception of an emotionally charged stimulus prompts
simultaneous but independent activation of both the autonomic nervous system and the emotional
response. The Cannon-Bard theory also draws attention to the neuroanatomical structures that trigger
the autonomic and emotional responses, with a particular focus on diencephalon structures such as
hypothalamus and thalamus.
Figure 58.2.
Theories of
emotion.
Comparison of
the
common-sense
interpretation,
the James-Lange
interpretation
and the
Cannon-Bard
interpretation of
emotions.
Papez Circuit
Just years later, in 1937, American neuroanatomist James Papez (pronounced payps) ascribed
emotional behavior to a particular series of brain structures. These structures, collectively called the
Papez circuit, consist of the hypothalamus, cingulate gyrus, thalamus, hippocampus, and more. The
model that Papez developed started with a stimulus that activates the thalamus, which in turn activates
a loop of activity between the hippocampus, hypothalamus, thalamus and cortex to produce both a
physiological response and emotional feelings.
Figure 58.3.
Papez Circuit.
The Papez circuit
is a series of
brain structures
(in pink) that are
responsible for
both emotional
feeling and
physiological
responses
following the
presentation of a
stimulus.
Papez observed unusual aggression among animals with injury to these structures, suggesting that
emotional responding is distributed across many areas, rather than localized. (Notably missing from
the Papez circuit structures is the amygdala, which was later added in a future revision in the 1950’s.)
Structures involved in emotion

Emotions are one of the most holistic functions of the brain, incorporating neural circuitry from
across several different structures, ranging from the phylogenetically older to the most advanced frontal
cortical areas. The structures of the Papez circuit are not a comprehensive list of brain structures
involved in emotional processing but offer a good starting place for describing the anatomy of emotion.
Amygdala
A limbic structure called the amygdala contributes heavily to processing the valence of emotional
experiences. Roughly the shape and size of an almond, the amygdala is part of the temporal lobe.
Generally, the amygdala is subdivided into several nuclei, including the basolateral amygdala, central
nucleus, and cortical nucleus. As a temporal lobe structure, amygdala is strongly implicated in
emotional memory formation. Emotional memories can be either positive valence (such as the
happiness you may have experienced at a birthday party when younger) or negative valence (such as
childhood trauma or being teased as a child).
Figure 58.4.
Amygdala. The
amygdala is a
bilateral structure
(highlighted in
red) that is
important in
emotional
responses.
Of the forms of declarative memory, autobiographical memories more often have emotional content
compared to semantic memories. Amygdala lesions have been used as a last resort treatment for
patients with temporal lobe epilepsy or psychiatric conditions with pathological and dangerous
aggressiveness. These psychosurgery strategies have been variably successful, but they often have high
complication rates and upwards of a 4% mortality rate. These treatments are rarely used today.
Hypothalamus
One of the major output signaling pathways of the amygdala is the hypothalamus, found at the
base of the brain. The hypothalamus is often seen as the neural structure that initiates endocrine
responses in the rest of the body, such as hormone production. Several different behaviors, ranging
from homeostasis, hunger, and circadian regulation are modulated by hypothalamic signals.
Figure 58.5.
Hypothalamus
and Pituitary.
The
hypothalamus is
located at the
base of the brain
(highlighted in
blue). The
hypothalamus is
connected to the
anterior and
posterior
pituitary through
the infundibulum
(orange). The
posterior
pituitary (purple)
releases oxytocin
and vasopressin
in the
bloodstream that
is produced by
hypothalamus.
Pituitary Gland
Downstream from the hypothalamus is the pituitary gland, a pea-sized endocrine organ that
protrudes from the base of the brain. It is strongly involved in the production and release of
neurohormones, signaling molecules produced by nerve cells that travel throughout the bloodstream
to influence the activity of several organs throughout the entire body. Recall that the pituitary gland
is subdivided into two regions with distinct anatomical and functional differences. Hypothalamic
magnocelluar neurons produce the neurohormones oxytocin and vasopressin. The axons of these
neurons project to the posterior pituitary gland, which then releases these hormones into the
bloodstream.
Oxytocin plays a significant role in the development and maintenance of prosocial behaviors, acts
such as trust, compassion, and empathy, all actions that enhance interpersonal relationships.
Interestingly, while oxytocin generally strengthens the social bonds between people, it promotes
antisocial behaviors against those not perceived to be within one’s own social group. Disorders of the
oxytocin system are believed to contribute to autism spectrum disorder and psychopathy, two complex
conditions characterized partly by social impairment. Some studies have examined the therapeutic
use of nasal oxytocin for a variety of psychiatric conditions, but the studies have been unable to
demonstrate strong clinical effects despite success in nonhuman animal models.
Insula
The insula (or insular cortex) is the lobe of the cortex buried deep within the lateral fissure. Although
not visible from a side view, the insula is often considered to be the fifth lobe of the telencephalon.
Early studies by Wilder Penfield where he directly stimulated the brain during open brain surgery led
researchers to suggest that the insula contributes to interoception, detecting the internal state of the
body and conveying that information for processing.
Figure 58.6.
Insula. The insula
can only be
visualized by
removing the
cortex outside
the lateral
fissure. Deep
within the cortex,
the insula
(highlighted in
this image) is an
important
structure for the
processing of
disgust.
In functional imaging studies, the insula is involved in the recall or many different emotional stimuli,
especially those emotions that have a sensory component. Notably, the insula is strongly implicated in
the emotion disgust. For example, a patient is placed in an fMRI scanner while breathing through a
mask, which allows the experimenters to change the smells that are perceived. Patients are then given
pleasant smells (such as passion fruit, pear, or mint), a neutral smell, or unpleasant smells (like ethyl-
mercaptan or isovaleric acid, which smells like skunk or body odor, respectively). There is increased
activity of the anterior insula in response to the unpleasant smells but not the pleasant smells.
The insula also responds to social cues related to disgust as well. When a patient in an fMRI sees a
video of a person smelling something unpleasant and reacting with a “repulsed” face (the closing of the
nostrils and curling of the upper lip), their anterior insula likewise increases in activity just as if they
had smelled it themselves. In addition to sensory stimuli, feelings of social repugnance (unwarranted
violence, murder) or moral disgust (incest) also increase insula activity. Atypical insula activity is
implicated in behavioral disorders. For example, insensitivity to disgust can lead to squalor-dwelling
conditions (sometimes seen in excessive hoarding or late cognitive decline), which puts those people
at heightened health risks due to regular exposure to unsanitary conditions. Substance use disorders,
PTSD, and suicide attempts have all been associated with atypical insula activity.
Key Takeaways
• Affective neuroscience studies emotions.

• There are seven basic classes of emotions: Anger, contempt, disgust, fear, happiness,
sadness, and surprise.
• The Papez circuit relates several brain structures to emotional feeling and
physiological feelings, including the amygdala, hypothalamus, pituitary gland, and
insula.
Attributions
Media Attributions
• Darwin Emotions © Charles Darwin adapted by Valerie Hedges is licensed under a Public
Domain license
• Emotion interpretations © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Papez circuit © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Amygdala © Life Science Databases adapted by Valerie Hedges is licensed under a CC BY-SA
• Hypothalamus © OpenStax College adapted by Valerie Hedges is licensed under a CC BY
• Insula © Schappelle adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
EMOTIONS: FEAR | 835
59.
EMOTIONS: FEAR
Nearly everyone has experienced the prototypical fear

response: Imagine reading a book when you see a spider
skittering along the wall. Suddenly, you’ll feel your heart
racing, your breathing increase, dryness of your mouth, Resources
and your palms sweating. You probably won’t notice the
dilation of your pupils or the change in liver activity and
digestion. This sympathetic nervous system activity is
downstream of being presented with a fearful stimulus. • Glossary terms
But upon closer inspection, it was no spider at all – just an • Scientist links to learn
errant piece of brown fuzz picked up by a draft. Within more
minutes, your body’s physiology returns back to normal. • Key takeaways
This anecdote points out a few important features
about the fear response. First, the onset of the fear
response is quick, and so is the dissipation of the fear.
Second, it is triggered by exposure to a perceived threat, regardless of whether the stimulus is a genuine
threat or not (the overwhelming majority of spiders are clinically harmless to humans!). Third, the fear
response is greatly modified by knowledge and experience – an entomologist would recognize that the
spider is a harmless house spider and would, instead of fear, display curiosity, interest, boredom, or
other emotions. On the other hand, someone who has been bitten by a dangerous spider and sent to
the hospital when younger would have a much stronger physiological response.
Fear is likely the most evolutionarily ancient emotion, and is highly protective. When encountering
a hungry mountain lion, faces displaying the traits of fear (enlarged eyes, flared nostrils, and a slightly
open mouth accompanying a gasp) would signal to others nearby that a threat is nearby, which helps
initiate heightened alertness and the appropriate fight-or-flight response.
836 | EMOTIONS: FEAR
Amygdala and Fear
Klüver-Bucy Syndrome
In 1939, two researchers named Heinrich Klüver and Paul Bucy described a unique set of emotional
deficits in monkeys with bilateral temporal lobe removal, including removal of the, further providing
evidence of the neuroanatomy of emotion. Most notably, the monkeys fail to display fear, including
their facial expressions and vocalizations even in the face of life-threatening stimuli such as a large
snake. Further they also had decreased anger or aggression. They also display visual agnosia (the
inability to recognize faces or objects visually), psychic blindness, hypersexuality, and hyperorality (an
inappropriate fixation with using the mouth to interact with surroundings, such as licking or eating
nonfoods). Collectively, these sets of symptoms are called Klüver-Bucy syndrome.
Although it is primarily an experimental manipulation observed in monkeys, some human patients
may develop the condition, such in after brain surgery, stroke, viral encephalitis, traumatic brain injury,
and many others. In addition to the symptoms described in Klüver-Bucy Syndrome, humans also
display “flattened” emotions. The lesioning of the amygdala clearly results in decreased expression
of fear. Stimulation of the amygdala, however, causes in increase in anxious, fearful and aggressive
responses in humans and other animals, such as piloerection in cats. In fact, amygdala activity is
increased when shown images of fearful faces, but not neutral or happy faces when measured with
fMRI.
Amygdala Lesions
Patient SM is a notable case study of a person who does not experience the fear response. Born with
an extremely rare genetic condition called Urbach-Wiethe disease, Patient SM progressively developed
calcification in her amygdala bilaterally, causing destruction and cell death. Like other people with
Urbach-Wiethe disease, she had no severe significant cognitive deficits, except for the inability to
experience fear.
In one test, she was shown a variety of emotionally charged videos, then asked to rate the intensity
of each clip with respect to different emotions. SM found clips from America’s Funniest Home Videos
to be just as funny as the control patients, and she found the clips of disgusting toilets to be just as
repulsive. But, when presented with clips depicting ghost hauntings or suspenseful serial killers on the
loose, SM rated these stimuli as being non fearful.
Other studies challenged Patient SM with more concrete threats. Researchers brought SM to a
pet store, where she asked to handle the snakes. She was stopped by an employee before she put
her hand into a tarantula cage out of curiosity. The researchers also took her to the Waverly Hills
Sanatorium haunted house, where she bravely led a group of strangers through the house populated
by actors dressed as monsters and ghosts. Although she did not display any fearful behaviors, such as
hesitation to walk through the darkened corridors, patient SM reported the sensation of exhilaration
and enthusiasm, akin to riding a roller coaster. She also was asked to recall some of her past real-life,
fear-provoking experiences, such as when she was attacked in a domestic violence incident or was held
up by a stranger at knife point in a public park. In none of these cases did she ever report feeling fear,
although she was upset and angered at the situation. The destruction of her amygdala seemed to make
her resilient against PTSD: the day after being threatened with a knife to her throat, she walked past
the very same park bench.
Types of Fear
Scientists have long realized there are at least two distinct types of fear:
• Innate fear in which subjects avoid certain stimuli such as snakes or spiders even though they
may never have seen them before
• Learned fear in which a stimulus or situation causes arousal or anxiety because it has been
associated with a painful or negative experience in the past
There are two important protocols used to examine learned fear: fear conditioning and
conditioned defeat.
Fear Conditioning
One of the best studied laboratory models of fear comes from the work of Joseph LeDoux who
studied the brain circuit that mediates learned or conditioned fear in laboratory rats. In these studies,
LeDoux used a classical conditioning procedure to induce what he called “conditioned fear”. In
classical conditioning (think Pavlov’s dogs), a neutral stimulus that normally would not cause any
physiological response (called a conditioned stimulus, e.g., a ringing bell) is paired with a meaningful
stimulus (called an unconditioned stimulus, e.g., the presence of food) that elicits a behavioral
response (unconditioned response, e.g., drooling). Eventually, the behavior (drooling) occurs in
response to the conditioned stimulus (bell) alone.
Instead of pairing the conditioned stimulus with a positive unconditioned stimulus like food,
LeDoux paired the conditioned stimulus with an electrical shock. After pairing the shock to the
stimulus multiple times, the animals responded to the conditioned stimulus alone (no shock) in the
same way they did to the electrical shock alone. This is referred to as the conditioned emotional
response.
Figure 59.1 Fear

conditioning. Left
panel: Prior to
conditioning, the
conditioned
stimulus (an
auditory tone)
has no effect on
behavior. The
unconditioned
stimulus (shock)
leads to
startle-like
behaviors that
researchers refer
to as “freezing”.
Middle panel:
During fear
conditioning, the
tone is paired
with the shock
and freezing
behavior is seen.
Right panel: After
successful fear
conditioning, the
tone alone can
elicit freezing
behavior. ‘Fear
Conditioning’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Conditioned Defeat
In this model the experimental subject (the animal whose behavior is being examined) is placed in the
home cage of a larger, resident animal. This typically results in aggressive behaviors displayed by the
resident animal toward the experimental subject. The resident animal will usually win the encounter
because it is larger and in its own territory. The experimental animals will show submissive and
defensive posturing and does not attack or threaten its opponent.
Experiencing this defeat has major long-lasting effects on the experimental subject. Following
defeat, the animal rarely shows aggression even to non-aggressive animals placed in the subject’s home
cage (an intruder would typically cause aggression). Because this paradigm has parallels with the
earlier fear conditioning studies that paired an acoustical tone with electrical shock to the feet, it is
often referred to as “conditioned defeat;” the experimental subject has been conditioned to respond to
all other animals with submissive defensive postures.
Figure 59.2.
Conditioned
defeat. Left
panel: Prior to
defeat, the
experimental
subject and
resident animal
are kept
separate, and
behavior is
normal. Middle
panel: During the
conditioned
defeat trial, the
experimental
animal is placed
into the home
cage of the
larger, resident
animal. The
resident will
defend its
territory, acting
aggressively
toward the
smaller,
experimental
animal. The
experimental
animal will show
submissive and
defensive
behaviors. Right
panel: After
defeat, the
experimental
animal will show
submissive
behaviors even
toward
non-aggressive
animals placed
into the
experimental
animal’s home
cage.
‘Conditioned
Defeat’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Fear and Aggression

Fear and anger are two very closely related emotions. As with many other emotions, anger manifests in
complex ways. The anger spectrum runs from low (irritation) to high (rage), and from quick (lashing
out) to persistent (vengeful). Anger can manifest behaviorally as aggression, though clearly in humans
we can feel angry without showing signs of aggression. In animal models, however, we can only judge
anger through their display of aggressive behaviors.
Aggression is affected by circulating levels of androgens, as castration (removal of the testes) can
reduce aggressive behavior. As with fear, amygdala activation is also related to aggressive displays, as
bilateral removal of the amygdala causes animals to be less aggressive and more docile. A type of
psychosurgery, amygdalectomy, bilateral surgical lesioning of the amygdala) can cause a “taming” effect
in humans, and is still performed today, though rarely.
In addition to amygdala circuits, regions in the frontal cortex decrease activity during acts of
aggression, suggesting that frontal circuits actively inhibit the limbic system, which drive our more
“primitive” responses. Researchers in the 1920s performed a series of lesion experiments, systematically
injuring different parts of the brain in cats. To their surprise, when the cortex of the cat was surgically
separated from the rest of the nervous system, a procedure called the decorticate preparation, the
cats would exhibit a hyper-aggressive response to stimuli. For example, an innocuous touch of the
tail would trigger violent clawing, biting, and hissing, behaviors which were described as sham rage.
The same sham rage behavior was observed following a decorticate preparation that also included
the anterior hypothalamus. However, when the decorticate preparation included both the anterior
and poster hypothalamus, sham rage was no longer observed. They concluded that rage (and other
powerful emotions) is normally under inhibitory control by the cortex. Altered frontal cortical action
may therefore account for one reason why two different people would react to the same anger-
provoking stimulus in different ways.
In a separate set of experiments, cats had different areas of their hypothalamus electrically stimulated
while being presented with a rat. These experiments indicated that stimulation of different areas of
the hypothalamus elicits distinct displays of aggressive behavior. When the medial hypothalamus
is electrically stimulated, the cat displays what is caused a “threat attack”. The threat attack includes
threatening behaviors such as hissing, but the cat did not attack the rat. If the lateral hypothalamus
was electrically stimulated, the cat displayed a “silent-biting attack”. In this case, the cats did not display
exaggerated threatening behaviors, but rather the cat would quickly and viciously attack the rat’s neck.
Key Takeaways
• Lesions of the amygdala in monkeys leads to Klüver-Bucy Syndrome, which includes

decreased expression of fear.
• Lesions of the amygdala in people results in decreased expression of fear, as
demonstrated with patient SM.
• Learned fear can be studied using either fear conditioning or conditioned defeat tests
• Fear conditioning pairs a neutral stimulus, like a light or a tone, to a harmful stimulus,
like a shock
• Conditioned defeat submits an experimental animal to aggressive behaviors from
another animal, after which the experimental animal will continuously show
submissive behaviors to others
Attributions
Media Attributions
• Fear Conditioning © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• Conditioned Defeat © Casey Henley
NEURAL CONTROL OF STRESS | 845
60.
NEURAL CONTROL OF STRESS
It is likely every reader of this chapter has experienced

some form of stress, perhaps due to a big exam, a looming
deadline, or an unplanned interaction with a spider. The Resources
physical reactions to stress, like increased heart rate and
breathing, are a result of brain activation.
• Key Takeaways
Types of stress • Test Yourself
Stress is often split into two categories: physical and

psychological. Physical stress can be caused by trauma,
illness, or injury. Blood loss, dehydration or allergic reactions are examples of physical stressors.
Psychological stress has an emotional and mental component. Fear, anxiety, and grief are examples of
psychological stress. The neural circuits involved in responding to the different stressors are
overlapping but separate.
Stress response systems

The body has two main systems for responding to stress: the autonomic nervous system and the
hypothalamic-pituitary-adrenal (HPA) axis. The autonomic nervous system response occurs very
quickly because it is synaptic in nature and is responsible for the “fight or flight” response, which
stimulates heart rate and breathing and inhibits digestion. The HPA axis is a hormonal response, so it
is a slower response relative to the autonomic system. Its downstream effects also promote energy use.
846 | NEURAL CONTROL OF STRESS
Neural Control
Hypothalamus
The hypothalamus plays a critical role in stress, activating both the autonomic and hormonal
responses. The hypothalamus is a region right above the brainstem on either side of the 3rd ventricle.
The hypothalamus manages hormone release in the body and maintains homeostasis; this small
structure is critical for numerous functions including hunger and thirst, temperature control,
regulation of blood composition, sleep, reproduction, and stress.
Figure 60.1. The

hypothalamus,
shown in orange
in this coronal
view, is located
adjacent to the
third ventricle.
‘Hypothalamus
Coronal View’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.

Although the hypothalamus directly controls the body’s response to stress, it is influenced by
activity in other regions of the brain. When information from the environment is processed, activity
is seen in the prefrontal cortex, hippocampus, and amygdala. These regions have direct and indirect
connections to the hypothalamus. The prefrontal cortex plays an executive decision-making role, the
hippocampus places events in context with previous memories, and the amygdala assesses a wide
range of stimuli for their potential ability to cause harm and places an emotional value on them.
Amygdala
The amygdala is located medially in the temporal lobe. The amygdala, which means “almond” in
Latin, is responsible for the processing of emotions and consolidating emotional memories. It is
especially active during fear learning and evaluates the salience, or importance, of a situation. For
instance, when we look at frightened faces, our amygdala is more activated than when we see neutral
faces. Conditions such as anxiety, depression, and post-traumatic stress disorder are all linked to
amygdala dysfunction.
The amygdala regulates the HPA axis by regulating the activity of the hypothalamus. Specifically, the
basolateral amygdala receives sensory information that is related to fear and processes that information
within the central nucleus of the amygdala, which then influences activity of the hypothalamus and
the stress response.
Figure 60.2. The

amygdala, shown
in yellow in a
coronal view, is
located in the
deep in the
anterior portion
of the temporal
lobe. ‘Amygdala’
by Casey Henley
is licensed under
a Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Hippocampus
Just posterior to the amygdala lies the hippocampus. The hippocampus, which means “seahorse” due
to the similarity between its shape and the animal, is important in the long-term consolidation of
memories, spatial navigation, and associating contextual cues with events and memories. Activity
within the hippocampus typically inhibits the HPA axis. When a stressor is encountered, the stressor
inhibits the hippocampus, thus disinhibiting the HPA axis and allowing for a stress response.
Stress has been demonstrated to damage the hippocampus in both monkeys and humans, causing
a decrease in the size of the hippocampus. This damage may be due to increased glucocorticoid levels
from higher than normal activation of the HPA axis. On a cellular level, increased glucocorticoid levels
alter the structure of hippocampal neurons in rodents, decreasing dendritic arborizations.
Figure 60.3. The

hippocampus,
shown in purple
in a coronal view,
is located in the
deep in the
temporal lobe.
Hippocampus by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
View the hippocampus using the BrainFacts.org 3D Brain
Prefrontal Cortex
Finally, the prefrontal cortex, which is located in the front of the brain in the frontal lobe, contributes
to higher level cognitive functions like planning, critical thinking, understanding the consequences of
our behaviors, and is also associated with the inhibition of impulsive behaviors. The prefrontal cortex
is one of the last brain regions to fully develop and may not be fully developed until an individual
reaches their mid-twenties. Experts think this might explain why teens are more likely than adults to
participate in risky behaviors.
Figure 60.4. The

prefrontal cortex
is located in the
anterior portion
of the frontal
lobe. ‘Prefrontal
Cortex’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
View the prefrontal cortex using the BrainFacts.org 3D Brain
Key Takeaways
• There are two systems that manage physical responses to stress: the autonomic
nervous system and the HPA axis
• The hypothalamus is located on either side of the 3rd ventricle, inferior to the
thalamus and directly controls both the autonomic nervous system and the HPA axis
• The amygdala, hippocampus, and prefrontal cortex all influence the activity of the
hypothalamus
Test Yourself!
Attributions
Media Attributions
• Hypothalamus Coronal © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• Amygdala © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Hippocampus © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Prefrontal Cortex © Casey Henley is licensed under a CC BY-NC-SA (Attribution
HPA AXIS | 851
61.
HPA AXIS
When presented with a stressor, our brain activates the

hypothalamic-pituitary-adrenal (HPA) axis, which
initiates a hormonal response. Resources
Hypothalamus
• Glossary terms
• Key Takeaways
The hypothalamus, which sits below the thalamus,
integrates information from many regions of the central
nervous system and plays a critical role in maintaining
homeostasis in the body. The hypothalamus regulates
temperature, hunger, thirst, blood volume and pressure, sleep and wakefulness, reproductive
functions, and stress and fear responses.
852 | HPA AXIS
Figure 61.1. The

hypothalamus,
shown in blue in
a mid-sagittal
section, sits
below the
thalamus, shown
in orange.
‘Hypothalamus
Sagittal’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Pituitary
The hypothalamic regulation of the body’s response to stress is managed via hormone release by the
pituitary gland. The pituitary gland is located inferior to the hypothalamus. The pituitary is divided
into two lobes, the anterior and the posterior pituitary. These regions are responsible for the
release of different hormones and are controlled by the hypothalamus in different ways.
HPA AXIS | 853
Figure 61.2. The

pituitary, shown
in green in a
mid-sagittal
section, lies just
below the
hypothalamus,
shown in blue.
‘Hypothalamus
and Pituitary’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
View the pituitary using the BrainFacts.org 3D Brain
Hormone Release
The stress response relies on anterior pituitary function. The hypothalamus contains two types of
neurons that secrete hormones into the pituitary: parvocellular neurosecretory cells and
magnocellular neurosecretory cells. Parvocellular cells are smaller than the magnocellular neurons
(parvus means “small” in Latin). In the HPA axis, the parvocellular neurosecretory cells release a
hormone called corticotropin-releasing hormone (CRH) into a specialized capillary system that
lies between the hypothalamus and the pituitary called the hypophyseal portal circulation. When
CRH reaches the anterior pituitary, it causes the endocrine cells of the pituitary to release
adrenocorticotropic hormone (ACTH) into the general circulation.
854 | HPA AXIS
Figure 61.3. In
response to
stress, the
hypothalamic
parvocellular
neurosecretory
neurons release
corticotropin-rele
asing hormone
(CRH) into the
hypophyseal
portal circulation,
causing the
hormone-releasi
ng endocrine
cells in the
anterior pituitary
to release
adrenocorticotro
pic hormone
(ACTH). ‘CRH and
ACTH Release’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
The ACTH travels through the circulatory system and can act on the adrenal cortex, a gland located
on top of the kidney. The adrenal cortex releases cortisol, a glucocorticoid hormone, into the blood
stream. Cortisol travels throughout the body and has many effects that prepare the body for either
fleeing or fighting the stressor. Promotion of energy use (for a quick escape or for defense) occurs
through the release of glucose, the sugar the body uses for energy.
HPA AXIS | 855
Figure 61.4. The

adrenal glands,
which sit on top
of the kidney,
release cortisol
into the
bloodstream in
response to
release of ACTH
by the anterior
pituitary. ‘HPA
Axis’ by Casey
Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
Hormone Action
Cortisol is a steroid hormone; steroid hormones are synthesized from cholesterol and are able to cross
the phospholipid bilayer because they are lipid soluble. Glucocorticoid receptors are located in the
cytoplasm of many cell types across the body. The receptors dimerize after cortisol binds, and the
dimer moves to the nucleus where it can alter DNA transcription.
Animation 61.1. Cortisol can cross the phospholipid bilayer and bind to glucocorticoid receptors.
The receptors dimerize, move to the nucleus, and interact with DNA, altering transcription of certain
856 | HPA AXIS
genes. ‘Cortisol Action’ by Casey Henley is licensed under a Creative Commons Attribution Non-
Commercial Share-Alike (CC BY-NC-SA) 4.0 International License.
Negative Feedback
Once the stress response has been initiated, and cortisol enters the circulation, cortisol itself is able to
act on the hypothalamus and pituitary and inhibit production of CRH and ACTH. This is called a
negative feedback loop; the active hormone (cortisol) can shut off its own production. Negative
feedback is possible because neurons in the hypothalamus and pituitary express glucocorticoid
receptors that are activated by cortisol.
Figure 61.5.
Cortisol released
by the adrenal
cortex inhibits
the synthesis and
release of CRH
and ACTH from
the
hypothalamus
and pituitary,
respectively. via a
negative
feedback loop.
‘Cortisol
Feedback’ by
Casey Henley is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike (CC
BY-NC-SA) 4.0
International
License.
HPA AXIS | 857
Regulation of the HPA Axis

One area of the brain that regulates the HPA axis is the hippocampus. Activity within the
hippocampus normally acts to inhibit activity of the HPA axis. Stressors in the environment inhibit
activity of the hippocampus, which in turn disinhibits the HPA axis and allows for cortisol levels to
increase. The hippocampus also expresses glucocorticoid receptors that bind to circulating cortisol and
act to increase hippocampal activity. This in turn increase inhibition of the HPA axis, another example
of negative feedback control within the circuit.
858 | HPA AXIS
Figure 61.6.
Hippocampal
regulation of the
HPA axis. The
hippocampus
normally inhibits
activity of the
HPA axis.
Stressors in the
environment
inhibit the
hippocampus,
which then
disinhibits the
HPA axis. Cortisol
released into the
circulation by the
adrenal glands
provides
negative
feedback within
this circuit by
binding to
glucocorticoid
receptors at the
level of the
anterior pituitary
gland, the
hypothalamus,
and the
hippocampus.
Cortisol inhibits
activity of the
anterior pituitary
and
hypothalamus,
thus decreasing
HPA activity.
Cortisol activates
the
hippocampus,
which allows it to
inhibit activity of
the HPA axis.
Cortisol therefore
HPA AXIS | 859
provides
negative
feedback within
this circuit.
‘Hippocampal
regulation of
HPA axis’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Chronic Stress
While this cortisol response to stress is particularly important in certain situations, like moments of
danger, chronic stress is an unhealthy scenario which can put people at risk for heart disease and other
illnesses. Chronic stress can cause structural and functional changes, like cell death or alterations in
the dendritic arbor, within the cortical regions that play a role in control of the HPA axis and the
hippocampus due to long-lasting exposure to cortisol.
Key Takeaways
• The hypothalamus directly controls the stress response by controlling hormone

release from the anterior pituitary
• The hypothalamus releases corticotropin-releasing hormone (CRH)
• The anterior pituitary releases adrenocorticotropic hormone (ACTH)
• The adrenal cortex releases cortisol
860 | HPA AXIS
• Cortisol binds to receptors and alters DNA transcription

• Cortisol can shut off its own production via a negative feedback loop
Attributions
Media Attributions
• Hypothalamus Sagittal © Casey Henley is licensed under a CC BY-NC-SA (Attribution

• Hypothalamus Pituitary © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• CRH and ACTH Release © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• HPA Axis © Casey Henley is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Cortisol Feedback © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• HPA axis with hippocampus © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
PSYCHIATRIC DISORDERS: ANXIETY | 861
62.
PSYCHIATRIC DISORDERS: ANXIETY
Psychiatric Disorders
The brain can be thought of as a well-oiled machine made
Resources
up of hundreds of billions of moving parts, all cooperating
in tandem for healthy behavior and function. There is
redundancy in the way these parts are organized, allowing
for occasional mishaps without any significant loss of
• Glossary terms
function. But when these parts interact in unusual or
• Key Takeaways
atypical ways, a person may develop some psychiatric
disorder. The conditions described in the next three
chapters likely involve dysregulations in molecules, cells,
or circuits, and are therefore complex.
As far as we know, the likelihood of developing these conditions is not exclusively determined by
either genes or influence from the environment. Instead, there is probably some influence from both.
That is to say, none of these conditions are 100% penetrant; none of them are dictated exclusively by
genetics. Having two parents or an identical twin with the condition may indicate an elevated baseline
risk over the population at large, but it is not a guarantee that the disease will manifest. Environmental
triggers and other exposures may lead to a sudden onset of the condition; on the other hand, certain
factors in the environment may be protective against these diseases.
One of the major challenges with understanding these brain diseases is related to the difficulty of
making an accurate diagnosis – as almost everything in biology exists on a spectrum, so do these brain
disorders. The symptoms of these disorders frequently overlap, adding another layer of complexity.
To help establish a diagnosis, the American Psychiatric Association (APA) has put together a series of
criteria for psychiatrists to diagnose these complex conditions. Recall our previous discussion of the
Diagnostic and Statistical Manual of Mental Disorders (DSM-5), used for compiling symptoms and
diagnostic criteria.
862 | PSYCHIATRIC DISORDERS: ANXIETY
Many of the treatments we currently have for neuropsychiatric disorders aren’t always effective.
Our ability to treat these conditions depends on our understanding of the disease. The better we
understand the causes of these conditions, the wider variety of new therapies we can test. Therapeutic
strategies for these conditions are often first tested using cells and non-human animal models, but these
have shortcomings. Most of the time, animal models of disease incompletely mimic the symptoms of
the disease. Unfortunately, we don’t have any animal models that reproduce the symptoms of the most
complex human conditions – it is almost impossible to create a mouse model of dissociative identity
disorder or dyslexia, and even if we could, scientists would struggle to quantify the behaviors that we
use as diagnostic criteria, which are too subtle to be observed or quantified in non-humans. And for
the animal models that we do have, they are often imperfect or incomplete, modeling only some of the
deficits seen in humans. Furthermore, most human diseases have many symptoms, and only a few can
be assessed with behavioral tests. We can only study disorders of the brain that have a clearly and easily
quantifiable behavioral component.
Anxiety Disorders
Anxiety is something that everyone has experienced at many points in their life. An anxious person
may experience cardiovascular symptoms such as elevation of blood pressure and heart rate, shortness
of breath, profuse sweating, and a state of panic. In many ways, the anxiety response is similar to
the fight-or-flight response observed during sympathetic nervous system activity. However, a clinical
diagnosis of anxiety is different from the passing anxiety that we all experience. Anxiety disorders are
characterized by fear, which is an adaptive response to an external threat or stressor.
Common Anxiety Disorders

Anxiety disorders can be very common, and lifetime prevalence estimates suggest 29% of people could
develop clinically significant anxiety over their life span.
There are various anxiety disorders. Some are described below.
1. Generalized anxiety disorder (GAD). People with GAD experience a constant sensation of
being overwhelmed, accompanied by fear and worry. Many times, this worry is not about a single
concern, but rather a combination of issues all at once, such as financial issues, relationship
issues, uncertainty of the future, and many others. GAD is much more severe and persists longer
than the normal worries that affect everyone. In GAD, worry persists for several months and is
uncontrollable. There are also associated cognitive symptoms, such as fatigue, irritability,
difficulty with concentration, and changes in sleep patterns.

2. Specific phobias. With specific phobias, a person develops the anxiety-related symptoms
(cardiovascular and psychological changes) in response to highly specific stimuli, such as snakes,
enclosed spaces, deep ocean, or public speaking. The person with the phobia perceives the
stimulus to be a great threat, even though it does not actually pose a genuine threat. Most people
with specific phobias will go to great lengths to avoid exposure to their particular phobia trigger.
These phobias are often influenced by social and cultural conditions. Developing a specific
phobia has a lifetime prevalence of about 7%, but only a very small number of people with
specific phobias ever seek treatment for their phobia. Like other forms of anxiety, there is a range
of severity of these phobias.
3. Panic disorder. A person with panic disorder experiences frequent panic attacks, characterized
by sudden increases in heart rate, shortness of breath, dizziness, and sudden numbness or
tingling (panic attacks can also be seen in specific phobias, but are not observed in GAD.) In
panic disorder, these panic attacks may occur independently of external influences.
4. Agoraphobia. Agoraphobia involves a fear of entrapment or of having no escape. Individuals
with agoraphobia may avoid crowds of people, riding elevators or cars, and crossing bridges.
5. Social phobia. Social phobia is similar to more specific phobias, however, with social phobia
there is a specific fear of public speaking or being in social situations.
6. Obsessive compulsive disorder (OCD). OCD is a common psychiatric condition affecting an
estimated 3% of the population, characterized by persistent intrusive thoughts or the need to
perform some ritualistic series of actions or motions. OCD exists on a spectrum, and in severe
cases, can severely impair the quality of an individual’s life.
7. Post-traumatic stress disorder (PTSD). Anyone can be diagnosed with PTSD at any point in
their lives. Some of the most common events that lead to a PTSD diagnosis include combat
experience, physical, emotional, or sexual assault or abuse, accidents, and natural disasters.
Women are twice as likely as men to be diagnosed with PTSD. Genetic influence appears to
account for approximately 30-40% of risk. The symptoms of PTSD are usually divided into
three categories: Re-experiencing, Avoidance, Hyper-arousal. Re-experiencing symptoms arise
when stimuli cause PTSD patients to relive their traumatic experience in flashbacks, frightening
thoughts, or nightmares. Avoidance symptoms arise when PTSD patients feel lack of emotion,
lose interest in activities they once enjoyed, or withdraw from family and friends. These
symptoms may be a result of trying to avoid reminders or triggers of the traumatic event. Hyper-
arousal symptoms appear as increased anxiety or feeling tense in safe environments. PTSD
patients can be easily frightened, may have trouble sleeping, and may have frequent angry
outbursts.
Theories of Anxiety Disorders

The exact cause of anxiety is still unknown. One theory suggests that anxiety is a maladaptive
evolutionary response to our modern living conditions. The argument is based on the observation that
an anxiety response looks a lot like a mild version of the fight-or-flight, sympathetic nervous system
response: both elicit cardiovascular and respiratory changes.
For the majority of the evolutionary history of Homo sapiens, we benefited from the sympathetic
nervous system as a reflex to improve the odds of survival in dangerous situations. However, our
modern civilized living conditions over the past few centuries have been very tame in comparison to the
risks that our earlier ancestors experienced. The relative ease of living has let the main function of the
sympathetic nervous system fall into disuse. The theory argues that people experience GAD because
a part of them encourages sustained activity in the sympathetic nervous system. Although thought-
provoking, this theory can’t be tested experimentally and offers no explanation about a biological
mechanism that can help to develop a therapy.
Treatments for Anxiety Disorders

There are various treatments for anxiety disorders.
1. Psychotherapy utilizes a therapist that performs exposure therapy for the individual to practice
being put in a situation that would normally cause anxiety and a fearful response to learn that
the situation is not actually dangerous. This treatment has been demonstrated to be very
effective but does take time and can be uncomfortable for the individual receiving the therapy.
2. Deep brain stimulation is a medical intervention in which a surgeon implants permanently
indwelling electrodes directly into brain tissue. These electrodes are controlled by an external
battery pack that delivers preprogrammed stimulation protocols. Deep brain stimulation has
been approved for treating obsessive compulsive disorder and is being tested as a potential
treatment for a number of other disorders.
Figure 62.1.
Deep brain
stimulation. A
deep brain
stimulator
device must be
implanted
surgically.
Electrodes are
positioned in
specific brain
areas for the
delivery of
electrical
impulses. The
impulses come
from an external
battery pack
that is typically
implanted
beneath the
skin on the
chest. Deep
brain
stimulation can
be used to treat
disorders such
as Parkinson’s
disease,
obsessive
compulsive
disorder, and
depression.
3. Psychosurgery involves an operation that uses a stereotaxic frame to make very precise
alterations to the brain by cutting connections between brain areas or by selectively lesioning
brain areas with the goal of correcting abnormal activity within the brain. Psychosurgery is still
performed today, but not very often.
4. Anxiolytic Drugs. Pharmacologically, there are a wide variety of drugs that can be used to treat
anxiety, broadly called anxiolytics. The first-line therapies are usually selective serotonin reuptake
inhibitors (SSRIs), the same class of compounds that are used in depression treatment. Other
anxiolytics, such as the benzodiazepines alprazolam or clonazepam, act as positive allosteric
modulators which increases the effect of the GABA system. Benzodiazepines are not always
preferred since they may have abuse potential and can be addictive. Opioids and norepinephrine
inhibitors can also decrease anxiety.
Figure 62.2.
Action of SSRIs.
Serotonin is
released from
synaptic vesicles
within the
presynaptic cell
and can then
cross the
synapse and
bind to
receptors on the
postsynaptic
cell. The
serotonin
transporter
(SERT) is
located on the
presynaptic cell
membrane.
SSRIs (red)
block the SERT,
causing
serotonin
concentration
within the
synapse to
increase, which
in turn increases
the possibility of
serotonin
binding to
postsynaptic
receptors.
When treating individuals for anxiety using drugs, the placebo effect is especially strong in those with
anxiety disorders. A placebo is an inactive substance that is not made to serve as a treatment, such as a
sugar pill. The placebo effect is a real response (can be either negative or positive) that an individual has
to receiving a placebo. Interestingly, the mechanisms underlying how the placebo effect works is still
largely unknown, but is believed to be related to the mind-body relationship.
SSRIs are the most common treatment for anxiety disorders. A SSRI inhibits reuptake of serotonin
by blocking the serotonin transporter (SERT) located on the presynaptic cell, effectively increasing
serotonin concentration within the synapse, and increasing the likelihood that serotonin will bind to
postsynaptic serotonin receptors. If increasing serotonin at the synapse is an effective treatment for
anxiety disorders, to many it logically follows that anxiety disorders are caused by decreased serotonin
levels. However, the cause of anxiety disorders is not so clear cut. It is possible that SSRIs might be
treating symptoms of the disorders rather than the actual underlying disorder. Alternatively, these
drugs may be producing compensatory changes within the brain that ultimately cause them to be
effective.
Animal Models to Study Anxiety

There are nonhuman behavioral tests used to assess anxiety in rodents, such as the elevated plus maze.
This maze is a raised platform, with four arms in the shape of a plus sign. Two of the arms have walls
surrounding the sides, while the other two are open, exposed on all sides. The rodent is free to move
between any of the arms as they choose. Standing in one of the open arms, where they can see the floor
far below them, is an anxiety-provoking condition. Under normal circumstances, rodents choose to
spend more time in the arms that are surrounded by walls. But if you give these animals an anti-anxiety
drug, they increase the time spent in the open arms, indicating a decrease in the behavioral expression
of anxiety.
Figure 62.3.
Elevated plus
maze. An
elevated plus
maze is used to
measure anxiety
in rodents. Two
of the arms are
enclosed and two
are open. When
an animal is
placed in the
middle of the
maze, a more
anxious animal
will spend most
of its time in the
closed arms of
the elevated plus
maze, whereas a
less anxious
animal will
venture out onto
the open arms of
the elevated plus
maze.
A related behavioral test is the open field test. The test apparatus consists of a large, flat area where the
rodent can move around freely, and some method to track the animal – either an aerial view camera or
a series of parallel invisible infrared beams that can locate the animal in the field. In the wild, rodents,
as prey animals, prefer to spend more time close to the sides of the testing arena up against the wall,
avoiding the wide-open space in the middle where their instinct warns them that they may be snatched
up by some predator. However, if you give the rodent an anti-anxiety drug, they will spend more time
venturing into the middle of the open field.
Another non-human model of anxiety is the predator exposure paradigm. In this paradigm, an
ethologically-relevant stimulus is presented to the rodent, such as one of their naturally occurring
predators. In this paradigm, rodent anxiety presents itself as a freezing response, an autonomic nervous
system activity spike, and a reduction in non-survival behaviors. Although the predator exposure
paradigm has good predictive validity, they may struggle with poor face validity, since the anxiety
measures also may appear as many of several other conditions, such as PTSD or stress.
Key Takeaways
• Psychiatric disorders are diagnosed through the DSM-5.

• An anxiety response is similar to sympathetic nervous system activity. Anxiety
disorder differ from passing anxiety due to an atypical expression of fear to an
environmental stressor.
• There are several different anxiety disorders that are all characterized by increased
fear.
• The cause of anxiety is largely unknown.
• Treatments for anxiety disorders include psychotherapy, deep brain stimulation,
psychosurgery, and anxiolytic drugs.
• Animal models are used to study anxiety, though they are not capable of completing
mimicking the symptoms of complex psychiatric disorders.
Attributions
Media Attributions
• Deep brain stimulation © NIMH adapted by Valerie Hedges is licensed under a Public Domain
license
• SSRIs © U3190069 adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• elevated plus maze © Samueljohn.de adapted by Valerie Hedges is licensed under a CC BY-SA
PSYCHIATRIC DISORDERS: AFFECTIVE DISORDERS- MAJOR DEPRESSION DISORDER | 871
63.
PSYCHIATRIC DISORDERS: AFFECTIVE

DISORDERS- MAJOR DEPRESSION
DISORDER
Affective Disorders
Affective disorders are highly prevalent. Affect indicates
Resources
mood, or emotional state. Therefore, affective disorders
are disorders of mood. We will cover two major mood
disorders: Major depression and bipolar disorder.
• Glossary terms
Major Depressive Disorder • Key Takeaways
Episodes of depression can occur throughout an

individual’s life with or without an external cause and can
last on the order of months or years. Depression is a highly prevalent condition with a lifetime risk of
about 18%. Depression gets diagnosed more frequently in women than in men, affecting about 5% of
women and 2.5% of men. Even with treatment, there is a high rate of relapse: an estimated 80% of
people with depression have more than one episode in their lifetime. The prevalence of depression is
similar across both high-income and low-income countries, indicating that biological factors
contribute significantly to the disease. Major Depression Disorder is also called unipolar depression to
differentiate it from the depression that represents a phase seen in bipolar disorder.
Clinical depression is often associated with another medical or psychiatric condition. For example,
rates of depression are higher among people with terminal diagnoses, like cancer. In this case, we say
that the two are comorbid. Additionally, a set of particularly challenging circumstances, like the death
of a loved one, could trigger a depressive episode. Major Depression Disorder represents a severe health
872 | PSYCHIATRIC DISORDERS: AFFECTIVE DISORDERS- MAJOR DEPRESSION DISORDER
risk across all ages. About 18% of adolescents report at least one instance of non-suicidal self-injury,
and the lifetime risk for suicide among people with Major Depression Disorder is estimated to be about
10%.
Symptoms of Major Depression Disorder

The fundamental criteria that are used to diagnose people with Major Depression Disorder is a
depressed mood/ self-esteem, low energy, and anhedonia, the decrease in sensitivity to pleasure.
Because of the decrease in pleasure, they have a lessened desire to engage in activities that once
produced happiness, thus leading them to become withdrawn from their friends and family. Short-
term changes in mood are completely normal and not clinical. The main diagnostic criteria for Major
Depression Disorder is the severity and duration of the symptoms. When the depression begins to
affect other aspects of life, including feelings of worthlessness, changes in sleep or appetite, difficulty
concentrating, or suicidal ideation that persist daily for two weeks or longer, then a person may be
diagnosed as clinically depressed.
To diagnose Major Depression Disorder, a trained psychiatrist or psychologist would use a
combination of interview with a self-report questionnaire such as the Hamilton Rating Scale for
Depression (HAM-D scale) or the Beck Depression Index. Items that appear on these sorts of tests
include: “Feels like life is not worth living” “Experience frequent weeping” “I blame myself all the time
for my faults” To date, there is no biomarker for depression.
Biological Basis of Affective Disorders
Monoamine Hypothesis
The monoamine hypothesis of affective disorders postulates that there is decreased signaling within
monoamine neurotransmitter systems and is based on observations in depressed patients. First,
treatment with different pharmacological substances has indicated that the monoamines serotonin
and/or norepinephrine are involved in affective disorders. The drug reserpine blocks the vesicular
monoamine transporter that is responsible for the loading of monoamine neurotransmitters into
vesicles prior to release at the synapse and causes severe depression in approximately 20% of people.
Monoamine oxidase inhibitors, drugs that inhibit the degradation of monoamine neurotransmitters
(therefore increasing monoamine availability) have been demonstrated to improve mood. Lastly, the
drug imipramine that blocks both the serotonin transporter and norepinephrine transporter is
effective at relieving depression. These pieces of evidence support that affective disorders are a
consequence of decreased serotonin and/or norepinephrine signaling.
Serotonin
Serotonin, specifically, has been a neurotransmitter of focus in depression since the 1950’s due to a
number of observations in depressed patients. There are lower levels of serotonin metabolites, such as
5-H1AA in the cerebrospinal fluid and diets that are low in tryptophan (the precursor for serotonin
synthesis) can cause a return of depressive symptoms. Lastly, a number of drugs that are effective
at treating affective disorders act to increase serotonin signaling and/or availability. However, it is
important to note that there is also evidence that opposes the theory that affective disorders are due
to a deficit in serotonin signaling. Primarily, that the level of serotonin in the synapse is less important
than how serotonin acts in the synapse. Plastic changes at the synapse, either presynaptically (altering
serotonin synthesis) or postsynaptically (altering expression and sensitivity of serotonin receptors)
affect how serotonin or serotonergic drugs act on the synapse.
Norepinephrine
There is also pharmacological data that supports a specific role for norepinephrine in affective
disorders. The drug desipramine specifically blocks the reuptake of norepinephrine at adrenergic
synapses and has been demonstrated to be effective at treating depression in some individuals. Recall
that norepinephrine and serotonin have different synthesis mechanisms, with serotonin synthesized
from tryptophan and norepinephrine synthesized from tyrosine. The drug AMPT inhibits the activity
of tyrosine hydroxylase, the rate limiting step of norepinephrine synthesis. For individuals that are
successfully treated for depression with the adrenergic drug desipramine, the administration of AMPT
results in worsening of depressive symptoms. However, when AMPT is administered to those on
serotonergic drugs, it has no effect on depressive symptoms. This evidence indicates that there may
be separate groups of individuals in which either serotonin or norepinephrine is the major
neurotransmitter system affected in depression.
Diathesis-Stress Hypothesis
Diathesis is a predisposition for a disease. The diathesis-stress hypothesis of affective disorders is
based on the observation that mood disorders have a tendency to be more common in families with
common genetics. This leads to some individuals having a genetic predisposition for the development
of mood disorders. Further, abuse and stress have both been demonstrated to be significant risk
factors for the development of mood disorders and anxiety and depression are often comorbid. In
the diathesis-stress hypothesis, the HPA axis (discussed in Chapter XX) is where both genetic and
environmental factors converge to ultimately cause mood disorders. Specifically, the HPA axis has
been shown to be hyperactive in depressed individuals, with increased levels of corticotropin releasing
factor (CRF), adrenocorticotropic hormone (ACTH), and cortisol and an overall decrease in negative
feedback control of the HPA axis.
Anterior Cingulate Cortex Dysfunction

The anterior cingulate cortex is a structure that is the anterior most portion of the cingulate
cortex just dorsal to the corpus callosum. It is part of the limbic system and typically functions in
emotions and in maintaining attention in healthy individuals. Interestingly, the anterior cingulate
cortex is interconnected with the HPA axis, the amygdala, prefrontal cortex, and hippocampus. When
individuals are sad or depressed there is increased activity within the anterior cingulate cortex, and
successful treatment of depression results in decreased activity of the anterior cingulate cortex. It
is hypothesized that dysfunction within the anterior cingulate cortex to prefrontal cortex circuit
underlies difficulty in concentrating on tasks during depressive bouts.
Figure 63.1
Anterior
cingulate cortex.
This MRI image
shows the
anterior cingulate
cortex
highlighted in
yellow in a
midsagittal view.
The anterior
cingulate cortex
is located
anterior in the
brain and dorsal
to the corpus
callosum.
Treatments for Major Depression Disorder

There is currently no completely effective treatment for Major Depression Disorder that reliably works
for everyone. The currently accepted strategies can be divided into behavioral treatments and chemical
treatments.
Cognitive Behavioral Therapy

Cognitive behavioral therapy (CBT) is a therapist-guided form of talk therapy that may help a
person manage their depression. In CBT, a patient’s behavior, including their coping mechanisms,
erroneous thoughts, and emotional responses, is analyzed through careful clinical examination and
patient self-reflection. Then, the patient is taught mechanisms to counteract those maladaptive
behaviors and replace them with adaptive behaviors. For example, a person undergoing CBT for Major
Depressions Disorder might learn to identify the moments when they dwell on something negative in
their lives, and then learn to tell themselves “that thought does not function to make my day better.
Let’s start the day by getting out of bed and see what happens next.” It should be known that CBT is
not exclusively for the treatment of Major Depression Disorder; CBT can also be effective for anxiety,
OCD, PTSD, insomnia, substance use disorders, behavioral addictions, and many others.
Pharmacological Treatments
A wide variety of drugs are used for the treatment of depression. The first-generation antidepressants
were developed in the 1950s and 1960s. These drugs acted to increase the action of the monoamine
neurotransmitters: primarily dopamine, norepinephrine, and serotonin. Our body uses an enzyme
called monoamine oxidase (MAO) which degrades these chemicals into inactive components that do
not signal at receptors. These first generation antidepressants block the action of MAO; biochemically,
we call them monoamine oxidase inhibitors (MAOIs).
In the presence of an MAOI, the neurotransmitter signal remains in the synapse longer, similar
to how an acetylcholinesterase inhibitor increases acetylcholine signaling. Most of the MAOIs, while
sometimes effective, have fallen out of fashion clinically because of the adverse side effects associated
with their biochemical activity. Some of them interact dangerously with foods rich in tyramine
(particularly fermented foods, such as aged cheeses or beer, as well as beans and processed meats), an
amino acid that is degraded by MAO. Excess tyramine can activate the sympathetic nervous system,
and body levels of tyramine can rise to dangerous levels in the presence of an MAOI, leading to adverse
cardiovascular events like stroke. Many of the common MAOIs, like phenelzine and isocarboxazid, can
be damaging to the liver. Some MAOIs also produce unwanted side effects, such as psychosis or nausea.
A different class of antidepressant drugs called the tricyclic antidepressants (TCAs), named for
the shape of their chemical structure, was also developed around this time. They generally act as
monoamine reuptake inhibitors, resulting in elevated neurotransmitter signaling. Unfortunately, these
tricyclics may produce many severe side effects, such as seizures, tachycardia, and heart attacks, so
prescriptions must be monitored closely. The tricyclics are still prescribed today for several other
nervous system disorders, ranging from insomnia to neuropathic pain, but due to their potential
cardiotoxicity, they are not often the first line of treatment in depression.
Our current, most often prescribed class of compounds for Major Depression Disorder, called the
third-generation antidepressants, are focused on boosting the signaling activity of serotonin. Instead
of preventing degradation, like the MAOIs, these compounds block the reuptake of serotonin out of
the synapse. These chemicals are called selective serotonin reuptake inhibitors (SSRIs), of which
fluoxetine (Prozac) is one of the most well-known examples. While SSRIs can be effective at reversing
the side effects of depression, they are not perfect drugs. One shortcoming is that a person needs to be
on the drug for 2-4 weeks before they start to experience a clinically meaningful reversal of depressive
symptoms. This finding is highly unusual since the pharmacological, molecular-level effects of SSRIs
take place within hours after taking the medication.
Similar to SSRIs, SNRIs (serotonin and norepinephrine reuptake inhibitors) can also be used
to treat depression. One of the unsavory side effects of SSRIs is serotonin syndrome, a set of somatic
changes resulting from excessive serotonergic signaling. In mild cases, a person may have an elevated
body temperature, excessive sweating, rapid heart rate, and elevated blood pressure. In more severe
cases, a patient may have severe fevers or seizures. Serotonin syndrome can happen in the event of an
SSRI overdose, or as a consequence of some interaction between an SSRI and other drugs like MAOIs,
MDMA (ecstasy), amphetamines, or cocaine.
Our newest treatment options for depression, recently approved by the FDA in March of 2019, is
esketamine, a dissociative anesthetic typically used as a veterinary tranquilizer and a recreational club
drug. Branded as Spravato, it can be administered rapidly via nasal spray. The strength of esketamine is
the speed of its action. After taking a dose, the antidepressant effects of the substance can be observed
within hours. The speed at which this drug takes effect, makes it ideal for treating severe cases of
suicidal thoughts.
One future therapy that is currently making significant medical advancements in curing depression
is the use of psychedelic drugs, particularly psilocybin – a substance found in some species of wild
mushrooms. As a potent serotonergic agonist, a single dose of psilocybin has been shown to decrease
depression scores in various self-report studies with little to no adverse side effects.
Electroconvulsive Therapy
For severe or treatment-resistant depression, electroconvulsive therapy (ECT) is a treatment option.
Introduced in 1938, the procedure has since been refined over the years (it is currently performed under
anesthesia) and is considered to be well-tolerated and highly effective. During the procedure, electrodes
are placed on the head and electrical current is passed through the electrodes. The mechanism of action
that accounts for the therapeutic effects of electroconvulsive therapy are not well-known. Side effects
include aches, nausea, and memory loss.
Figure 63.2.
Electroconvulsive
therapy.
Electroconvulsive
therapy delivers
an electric
current via
electrodes
through the skull.
Deep Brain Stimulation

Deep brain stimulation that targets the cingulate cortex or the prefrontal cortex that may be
overactive in depression, is ultimately aimed at decreasing activity. This treatment is only pursued
when other less invasive treatments are unsuccessful.
Animal Behavioral Tests of Depression

Behavioral neuroscientists have developed a variety of tests to assess the effectiveness of antidepressant
drugs in non-humans. They are roughly divided into two categories: despair-based tests and reward-
based tests.
Despair-based tests
One symptom of depression is that a person “gives up,” a behavior that can be modeled in a variety
of rodent tests. One such example is in a tail suspension test. In this test, a rodent is held by the tail
upside down. While it does not cause injury to the animal, they do experience discomfort, and will
generally struggle to either free themselves or to get upright again. The sooner they stop struggling
(more time spent immobile), the more despair they experience, or the more depressive they are. Giving
a rodent an antidepressant like fluoxetine causes them to fight for a longer duration.
Figure 63.3. Tail

suspension test.
A tail suspension
test is an
example of a test
of despair. In this
test, a rodent is
suspended by its
tail. This is
typically
uncomfortable
for the rodent,
which will
struggle to get
free. When
animals struggle
for very little
time, it is
interpreted as a
measure of
despair or giving
up.
A similar test to assess “giving up” is the forced swim test. Here, a rodent is put into a container
with some water. While they are naturally buoyant and are not at risk of drowning, they do not like
being wet and will try to swim so that they can climb out of the water-filled container. As in the tail
suspension test, they are unable to escape their predicament, and will eventually become immobile.
Giving them an antidepressant decreases time spent immobile.
Figure 63.4.
Forced swim test.
In a forced swim
test, another
example of a test
of despair, a
rodent is placed
in a container of
water. The
rodents do not
like to be wet,
and will try to
escape the
container.
Animals that
stop trying to
escape will
eventually float
in the water
(immobile). A
longer latency to
float indicates
more despair.
Reward-based tests
These tests seek to measure the severity of anhedonia, one of the main symptoms of depression. For
example, a rodent may be presented with a two-bottle choice task, where they are put into a cage
with two different bottles to drink from: one filled with standard water, and the other filled with a
more desirable sugar-water solution. A depressed rodent will not drink from the sugar-water bottle as
frequently as a healthy rat but give that rat an antidepressant, and they will prefer the sweet water. An
intracranial self-stimulation paradigm can also be tested here in the context of depression. When
an electrical stimulator is placed in a reward area of the brain and the rodent is trained to perform some
operant task to receive activation of these areas, we find that depressed rodents do not activate these
areas of their brain as much as rodents on antidepressants.
Key Takeaways
• Affective disorders are disorders of mood and include Major Depression Disorder and
Bipolar disorder.
• Symptoms of depression include a depressed mood/ self-esteem, low energy, and
anhedonia.
• Monoamine neurotransmitter systems, especially serotonin and norepinephrine, have
been implicated in depression. With decreased signaling in either serotonin or
norepinephrine signaling underlies the monoamine hypothesis of depression.
• Hyperactivity of the HPA axis has been observed in depression and underlies the
diathesis-stress model of depression.
• Hyperactivity in the anterior cingulate cortex has been observed in depression.
• Treatments for depression include pharmacological treatments, cognitive behavioral
therapy, electroconvulsive therapy, and deep brain stimulation.
• Depression is modeled in rodents through tests of despair and tests of reward.
Attributions
Media Attributions
• Anterior cingulate cortex © Geoff B Hall adapted by Valerie Hedges is licensed under a Public
Domain license
• electroconvulsive therapy © BruceBlaus adapted by Valerie Hedges is licensed under a CC BY-SA
• Tail suspension test © DataBase Center for Life Science (DBCLS) adapted by Valerie Hedges is
• forced swim test © DataBase Center for Life Science (DBCLS) adapted by Valerie Hedges is
PSYCHIATRIC DISORDERS: AFFECTIVE DISORDERS- BIPOLAR DISORDER | 883
64.
PSYCHIATRIC DISORDERS: AFFECTIVE

DISORDERS- BIPOLAR DISORDER
Bipolar Disorder
A person with bipolar disorder (BD) experiences phases
Resources
of clinical depression as described in the previous chapter,
and at other times they experience mania, a state of
exhilarating high energy. During this state, they may sleep
very little, have difficulty concentrating, and experience
• Glossary terms
pressure of speech: a perceived need to speak very rapidly
• Key Takeaways
to get their thoughts out. They might make poor financial
or life decisions.
Historically, BD has been called “manic depression.”
The estimated prevalence of BD is around 2.5%, but the disease is often misdiagnosed in the clinic as
Major Depression Disorder. One reason this happens is that more people are aware of the symptoms
of depression, and these symptoms are generally more easily observed. Mania is more difficult to
identify, since in mild cases it may be hard to distinguish from a person just “being in a really good
mood.”
Diagnosis of Bipolar Disorder

For a diagnosis of BD according to the DSM-V, a mood cycle has to last for a week or more. The
word “bipolar” is often misused in pop culture. Frequent changes in mood from happy to sad does
not characterize BD. In fact, for a person to be diagnosed with rapid-cycling bipolar disorder, they
need to experience four mood transitions annually! BD is usually diagnosed in adolescence and early
884 | PSYCHIATRIC DISORDERS: AFFECTIVE DISORDERS- BIPOLAR DISORDER
adulthood. As with depression, there are some genetic factors involved, since a family history of BD is a
risk factor. Concordance rates for identical monozygotic twins are estimated to be between 35%-80%.
But, environmental influences may be the precipitating factor in the onset of BD.
BD is diagnosed into two categories, based roughly on the severity of symptoms. Bipolar 1
disorder is the more severe of the two conditions, with a clinical diagnosis made when a patient
experiences depressive or manic events that cause significant social or occupational impairment, or
hospitalization to prevent serious self-harm. A diagnosis of bipolar 2 disorder is less severe, but the
behavioral changes are still noticeable by friends and family. A related diagnosis is cyclothymia, where
a person has alternating mood states that shift from depression to hypomania, a less severe state of
mania. Like most other disorders, BD exists on a spectrum, and these labels only exist for simplicity.
Figure 64.1.
Cycles of
depression and
mania in bipolar
disorder. In
Bipolar 1 disorder,
individuals cycle
between states
of mania and
major depression.
In Bipolar 2
disorder,
individuals cycle
through a less
severe form of
mania (called
hypomania) and
major depression.
Lastly, a related
disorder, in
Cyclothymia
patients cycle
between
hypomania and
minor
depression.
PSYCHIATRIC DISORDERS: AFFECTIVE DISORDERS- BIPOLAR DISORDER | 885
Treatment of Bipolar Disorder

The main issue with BD therapy is bringing the patient to some “middle” state: an antidepressant may
treat the depression phase, but could also swing the patient into mania. Similarly, a mania-controlling
drug could initiate depression.
Currently, our most reliable therapy for BD is lithium drugs. These compounds are described as
mood stabilizers since they act to move the patient’s mood to the center, rather than being at either the
high end of mood (mania) or the low end (depression). The way lithium acts to reverse the symptoms
is still unknown, and it probably acts on multiple pharmacological targets.
The main downside of this therapy is that lithium is very toxic. It has a very narrow therapeutic
window: blood levels of lithium lower than 0.6 mEq/L produce no effect, and anything above 1.5
mEq/L causes delirium, tremor, fatigue, and deadly side effects like seizures and coma. It is also
harmful to the kidneys after long exposure. Therefore, a person taking lithium drugs regularly undergo
therapeutic drug monitoring, a procedure by which the concentration of lithium is assayed.
Therapeutic drug monitoring requires frequent visits to a hospital. Usually, patients get multiple blood
draws in the first month when they start lithium treatment, decreasing to one test every 2 months,
before decreasing to about four times a year.
Bipolar disorder is very challenging to model in nonhumans. A genetically modified disruption of
the circadian rhythm can induce mania-like symptoms, as can extending the length of daytime light
exposure; oppositely, decreasing daily light exposure can induce depressive behaviors. Exposure to
amphetamine can increase manic behaviors, while withdrawal from the drug can induce depression.
Clearly, neither of these models for BD exhibit strong validity.
Key Takeaways
• Bipolar disorder is an affective (mood) disorder.

• Bipolar disorder has periods of both depression and a high energy state, called mania.
886 | PSYCHIATRIC DISORDERS: AFFECTIVE DISORDERS- BIPOLAR DISORDER
• Lithium drugs act as mood stabilizers. Through an unknown mechanism, lithium is

effective at treating bipolar disorder, however, lithium is very toxic and patients need
to be monitored for toxicity levels.
Attributions
Media Attributions
• bipolar disorder © Blacktc adapted by Valerie Hedges is licensed under a CC BY (Attribution)

license
PSYCHIATRIC DISORDERS: SCHIZOPHRENIA | 887
65.
PSYCHIATRIC DISORDERS:
SCHIZOPHRENIA
Schizophrenia is a psychiatric condition affecting just

under 1% of people. Schizophrenia affects men slightly
more often than women and affects people of all races.
There is a strong association between low socioeconomic Resources
status and the risk of developing schizophrenia, indicating
that stresses such as neonatal nutritional deficiency or
food insecurity may be risk factors. Other risk factors that
contribute to increased schizophrenia risk include • Glossary Terms
prenatal drug exposure, heavy drug use during early • Links to learn more
adolescence, and childhood adversity. • Key Takeaways
A diagnosis is generally made while a person is in their
late adolescent years through their thirties. During this
phase of life, the brain is still undergoing subtle
maturation processes, which may account for why a person is more vulnerable in these years. After this
age, the risk of developing schizophrenia decreases significantly. Also, the later in life that schizophrenia
symptoms appear, the better the health outcomes are. It is worth noting that people with
schizophrenia have the neurotypical range of intelligence, with the occasional outliers: John Nash, the
real-life Nobel prize-winning economist depicted in the movie “A Beautiful Mind”, was first diagnosed
with schizophrenia in 1959.
Symptoms of schizophrenia
The symptoms of schizophrenia can be roughly classified into two categories: positive symptoms and
negative symptoms. These phrases are used to describe whether there is an excess of some function
888 | PSYCHIATRIC DISORDERS: SCHIZOPHRENIA
(positive symptoms) or a deficit of a function (negative symptoms). The symptoms do not appear
uniformly across patients, so not all patients develop every symptom.
Positive Symptoms
The most well-known positive symptom of schizophrenia is hallucinations, perceiving something
that is not there (as opposed to illusions, which are misinterpretations of things that are there). Usually,
patients experience auditory hallucinations, but they more rarely have visual hallucinations. The
voices that people hear may be consistent or can change over time. Interestingly, the nature of these
hallucinations is influenced by society. In cultures with strong ancestor reverence, they may hear the
voices of their grandparents, whereas people in religious cultures may hear the voices of deities.
Relatedly, people with schizophrenia may experience a variety of delusions, untrue beliefs that
cannot be changed despite overwhelming evidence. The delusions can come and go spontaneously.
Delusions exist in many forms. A paranoid delusion is when a person believes that they are being
spied on, maybe by the government or by aliens. A persecutory delusion is a persistent thought that
the world is out to get them or to do them harm. Delusions of grandeur are when a person has a
tremendously high sense of self esteem, believing that they are royalty or are the reincarnation of God.
Other positive symptoms that can present in schizophrenia are a variety of motor disturbances.
Basal ganglia and cerebellar structural deficits are found in schizophrenia, two brain structures involved
in motor control, which may explain why deficits are observed. One motor difficulty is catatonia,
where a person can hold their body in a highly unusual position for a prolonged period of time. They
may also display stereotypy, a series of repetitive, purposeless behaviors, such as the persistent rocking
of the body or self-caressing.
Figure 65.1.
Individual with
catatonia. This
picture shows an
individual with
catatonia holding
their body in a
rigid and unusual
position for long
periods of time.
Negative Symptoms
The negative symptoms of schizophrenia may include deficits in expression. One common symptom
is a flat affect, where a patient does not show or express emotion in situations where you would
expect to see them. A related negative symptom is alogia, a decrease in the use of language. People
with alogia often use vague language that is lacking in content or repetitive. Negative symptoms also
include deficits in motivation or interest. Two closely-related negative symptoms include anhedonia,
a loss of a sensation of pleasure and the inability to expect upcoming pleasure. Furthermore, patients
with schizophrenia may also exhibit avolition, a decrease in goal-directed activity, which can cause
a person to stop seeing their friends and cease displaying interest in social gatherings, leading to
worsened interpersonal relationships. Negative symptoms may also manifest as a deficit of a patient’s
cognitive abilities, particularly shortcomings in episodic memory. They may also present with difficulty
in performing attention-related behavioral tasks.
Biological Basis of Schizophrenia
Genetics
Schizophrenia has a strong genetic component, with increased risk associated with having a first-degree
relative that has the disease, however most individuals with schizophrenia do not have a family history.
Many different genes have been identified in disease onset and progression.
One example supporting the genetic component of schizophrenia are the Genain Quadruplets.
They were a set of identical quadruplet sisters that were born in 1930. All four of the sisters developed
schizophrenia by the age of 24. The sisters’ paternal grandmother may have had schizophrenia and
it was known that they grew up in an abusive household, making it unclear if their development of
schizophrenia stemmed from their environment, genetics, or a combination of the two.
Structural Abnormalities in Schizophrenia

A number of structural abnormalities have been observed in the brains of individuals with
schizophrenia. For one, the lateral ventricles are typically enlarged in those with schizophrenia than
in those without schizophrenia. The increased size of the lateral ventricles could alter intracerebral
pressure or compress the areas of the brain adjacent to the lateral ventricles.
Figure 65.2.
Enlarged
ventricles in
schizophrenia.
The lateral
ventricles
(highlighted in
purple) are
enlarged in
individuals with
schizophrenia
compared to
individuals
without
schizophrenia.
In addition, a decrease in cortical thickness has been observed as well as wider than typical sulci between
the gyri. Problems of cell migration during development may also play a part in schizophrenia, leading
to abnormal cellular densities and organization. There also seems to cell loss and disorganized cell
location within the prefrontal cortex.
Role of Dopamine in Schizophrenia

In a healthy person, dopamine is important for motor control and motivation, two behaviors that are
changed in patients with schizophrenia. Dopamine receptors (D1 and D2-like receptors) are found
throughout the mesolimbic and nigrostriatal dopamine pathways. Therefore, scientists have suggested
that increased dopamine signaling may be an underlying root cause in schizophrenia. In fact, psychotic
episodes are triggered by activation of dopamine receptors and increased D1 receptor binding has been
observed in the prefrontal cortex in those with schizophrenia. While the dopamine hypothesis is one of
the earliest theories of schizophrenia, modern genetics studies have shown that polymorphisms in the
dopamine D2 receptor are risk factors.
Figure 65.3.
Dopamine
pathways in the
brain. There are
two major
dopaminergic
pathways in the
brain. The
mesolimbic
dopamine
pathway starts
with
dopaminergic cell
bodies in the
ventral
tegmental area
(VTA) that
extend axons to
the nucleus
accumbens and
prefrontal cortex
to control reward
and motivation.
The nigrostriatal
dopamine
pathway has cell
dopaminergic cell
bodies in the
substantia nigra
that extend
axons to the
striatum to
control motor
behavior.
Animal Models of Schizophrenia

One animal model for schizophrenia is based on the hypothesis that excess dopamine leads to the
disorder. Introducing high doses of the drug amphetamine, which increases dopaminergic signaling,
induces a temporary schizophrenic-like state in non-human animals. The hyperdopaminergic model
of schizophrenia produces cognitive deficits with no changes in memory or other negative symptoms.
Alternatively, administration of NMDA glutamate receptor antagonists, like ketamine or PCP, is also
used as a behavioral model of schizophrenia.
Other non-human models of schizophrenia are neurodevelopmental models. In these models, a
pregnant dam is exposed to the compound MAM, which causes the newborns to develop atypically
and display behavioral deficits similar to schizophrenia. Inducing an unusually strong immune
response in the pregnant mother can also cause atypical development in utero, which causes the
animals to experience behavioral deficits after birth. The biggest limiting factor to developing an
animal model is that many symptoms in human schizophrenia, like paranoid delusions or auditory
hallucinations, are impossible to detect and quantify in nonhumans. The PCP model can cause
changes in rodent social behaviors, but it is hard to tell if this model causes any of the positive
symptoms that you might see in a patient with schizophrenia. Despite the limitations of these non-
human models of schizophrenia, they have been helpful in testing the therapeutic efficacy of anti-
schizophrenia drugs.
Treatments for Schizophrenia
Pharmacological Treatments
The dopamine theory of schizophrenia has led to a few novel therapeutic strategies, especially in the
context of the dopamine D2 receptor.
Typical, or classical, antipsychotics such as thorazine or haloperidol are more effective treating
positive symptoms of schizophrenia such as hallucinations and delusions in some patients. These drugs
block D2 dopamine receptors (dopamine antagonists) and decrease dopaminergic signaling, however
they have many undesirable motor side effects such as tremors, decreased voluntary movement, tardive
dyskinesias, and a shuffling gait named the ‘Thorazine shuffle’. The effectiveness of the antagonist is
correlated with the ability of that drug to block the D2 receptor.
Atypical, or nonclassical, antipsychotics such as Clozapine, antagonize a wider array of
dopamine receptors in addition to some serotonin and norepinephrine receptors. These drugs more
effectively treat negative symptoms of schizophrenia, but also can treat the positive symptoms with
less side effects compared to the typical antipsychotics. Unfortunately, pharmacological therapies are
not always effective in humans. Around a third of patients discontinue their treatment regimen, and
around a fifth of them report adverse side effects such as extrapyramidal motor symptoms, sedation,
and weight gain.
Transcranial Magnetic Stimulation

A potential new therapy is based on transcranial magnetic stimulation. In physics, there is a
close relationship between electricity and magnetism. The movement of magnets can generate electric
currents, and conversely, electricity can generate magnetic fields, a process called induction. A brain
stimulation technique called transcranial magnetic stimulation (TMS) relies on induction to generate
electrical currents through the skull. The TMS machine itself is a handheld coil of wires in a loop.
Passing electrical current through that loop produces a magnetic field. The magnetic field generated
then induces an electrical current at some distance away from the coil. By placing the coil at the surface
of the scalp, we can electrically activate small areas of brain tissue.
Figure 65.4.
Transcranial
magnetic
stimulation.
Transcranial
magnetic
stimulation
(TMS) is a
noninvasive
technique used
to alter electrical
activity in the
brain. The
machine is a
handheld coil
produces a
magnetic field
that induces an
electrical current
in the tissue near
the coil. The coil
is placed over the
scalp to alter
activity of the
brain tissue
beneath it.
For instance, using TMS above the motor cortex causes muscle contractions, and using TMS above
the occipital lobe causes the perception of flashes of light. A single pulse of TMS delivered to the
right motor cortex will produces electrical activity in the motor nerve of the left hand, causing it to
twitch. In repetitive TMS (rTMS) multiple pulses are delivered, which has been demonstrated to
produce long-lasting effects that extend beyond the stimulation period. rTMS can be used to produce
either inhibition in brain targets, through low frequency rTMS, or to produce excitation to the brain
through high frequency rTMS.
TMS is believed to have moderate benefits for several different psychiatric conditions, potentially
making it a meaningful therapeutic intervention. Single pulse TMS can be used to evaluate damage
from stroke, ALS, multiple sclerosis, and a number of motor disorders. It has also been approved
for the treatment of migraines. rTMS delivered to the motor cortex may help alleviate chronic pain
conditions, Parkinsonian symptoms, and improve contralateral motor function following stroke
injury. Further, rTMS activation of prefrontal cortex can decrease anxiety, antidepressive symptoms,
cigarette craving or consumption, and schizophrenia. Stimulation of other brain regions can be
antiepileptic and may also minimize auditory hallucinations or tinnitus (ringing in the ears.) In
schizophrenia, some evidence suggests that targeted activation of the cortex can decrease the severity of
auditory hallucinations. There may also be some mild improvements in the negative symptoms.
The technique itself is completely noninvasive. TMS can be delivered simply with placement of
the electric coil on the surface of the head. TMS may have some unexpected consequences, such
as temporary headaches, localized pain, changes in hearing, and bizarre changes in somatosensation.
The most severe side effect so far recorded is seizures, which can be very dangerous. Use of TMS is
still highly experimental. As with fMRI, the magnetic fields generated by a TMS device can cause
dangerous interactions with any magnetosensitive implants, such as deep brain stimulation devices,
cochlear implants, or aneurysm clips.
Figure 65.5.
Demonstration of
transcranial
magnetic
stimulation. This
picture shows a
person
undergoing
transcranial
magnetic
stimulation
therapy. The
magnetic coil is
placed over the
skull and it can
alter electrical
activity of the
underlying brain
tissue.
Key Takeaways
• Schizophrenia is a psychiatric condition that has both genetic and environmental

components.
• The symptoms of schizophrenia are classified into two categories: positive symptoms
(addition of function) or negative symptoms (subtraction of a function).
• A number of structural abnormalities have been observed in schizophrenia.
• There are animal models that help model some of the symptoms of schizophrenia,
but modeling hallucinations and delusions is not possible in animal models.
• Excess dopamine signaling has been implicated in schizophrenia and pharmacological
treatments are aimed at decreasing dopaminergic signaling.
• Transcranial magnetic stimulation is a newer technique that is being used to treat a

number of different psychiatric disorders.
Attributions
Media Attributions
• catatonia © ункноњн adapted by Valerie Hedges is licensed under a Public Domain license
• enlarged ventricles © BruceBlaus adapted by Valerie Hedges is licensed under a CC BY-SA
• dopamine pathways © NIDA adapted by Valerie Hedges is licensed under a Public Domain
license
• TMS © Eric Wassermann, M.D. adapted by Valerie Hedges is licensed under a Public Domain
license
• TMS on person © Eric Wassermann, M.D. adapted by Valerie Hedges is licensed under a Public
Domain license
MEMORY SYSTEMS | 897
66.
MEMORY SYSTEMS
Think back to your favorite birthday party. Which of your

friends were there? What did you do, where did you go, Resources
and did you have cake? Did you get gifts? The ability to
perform this task depends on our ability to create and
recall memories. According to our current best
• Glossary Terms
understanding of the neuroscience of learning, the
underlying biology of a memory mainly consists of subtle
more!
changes among synapses distributed across several brain
• Key Takeaways
areas. Our ability to learn new facts, recount the events of
last week, or to perform new motor skills is the result of
learning-induced neural plasticity. In our discussion of
learning and memory, we will consider different aspects of learning and memory, starting from the
behavioral level down to the molecular changes responsible for memory formation.
Types of Memory
First, it is important to distinguish between different types of memories.
898 | MEMORY SYSTEMS
Figure 66.1.
Different types of
Memory.
Declarative
memory,
nondeclarative
memory and
working memory
are distinct from
each other.
Declarative
memories, also
called explicit
memories, are
memories of
piece of
information that
can be stated
explicitly.
Declarative
memories can be
further
subdivided into
semantic
memory,
memory of facts,
and episodic
memory,
memory of
autobiographical
events.
Nondeclarative
memories
encompass
“knowing how”
memories and
include
procedural
memories,
emotional
memories, and
skeletal muscle
memories.
Working
memory involved
temporarily
storing
information that
you are
simultaneously
working on.
‘Types of
Memory’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution-NonC
ommercial-Share
Alike 4.0
International
License.
Declarative Memory
Declarative memories, also called explicit memories, are the pieces of information that can be
consciously declared or stated explicitly. Declarative memories are thought of as a “knowing what”.
Declarative memories can be further subdivided into semantic memory and episodic memory.
Semantic memories are pieces of factual information. Some examples include: 1. “Jupiter is the largest
planet of our solar system.” 2. “Rosalind Franklin discovered the doublehelix structure of a DNA
molecule.” 3. “The actor Keanu Reeves played the protagonist of the movie The Matrix.”
An episodic memory, sometimes also called an autobiographical memory, is the recollection of a
discrete moment in a person’s life. It can be thought of as “mental-time travel” – what was it like when.
The following memories are examples of episodic memories: “When I got home, I put my wallet and
phone on the table.” “I ordered pizza last night.” “In 2019, I went to see my favorite musicians perform
live.”
Declarative memories can either be a short-term memory or a long-term memory, which are distinct
from each other. The process of memory consolidation takes facts and events and converts them
from short-term memory into a long-term memory, which is supported by structural and functional
change within the neurons that hold the long-term memory.
Nondeclarative Memory
Nondeclarative memory encompasses all other types of memory outside of semantic and episodic
memory including procedural memories, skeletal muscle memory, and emotional response memory.
Procedural memories, (or implicit memories) are unconscious memories, and can’t be explicitly
stated. These can be thought of as “knowing how”. Some examples of procedural memories include,
for example, performance of a series of motor actions without conscious thought such as an
experienced musician playing a simple scale (sometimes commonly called “muscle memory”, even
though the muscles do not store any actual memory!), or a priming effect (such as when a person
sees pictures of bananas, they are more likely to answer the fill-in-the-blank prompt “b _ _ _ _ _” with
“banana”, whereas other people might guess “bubble” or “badger.
There are two different types of procedural learning: non-associative learning and associative
learning.
Non-associative learning
Non-associative learning involves a change in behavioral response over time in response to a single
type of stimulus and includes habituation and sensitization. In habituation, responses decrease after
repeated presentation of a stimulus as the individual learns that the stimulus lacks meaning. For
example, if someone poked you in the back, you will initially jump in surprise. However, if someone
repeatedly pokes you in the back over and over again, your response will decrease and you realize that
the stimulus is meaningless.
In sensitization, responses increase following presentation of a strong stimulus. For example a
sound of a door opening in your house typically will not cause a strong response. However, if you are
home along and hear a loud bang followed by a door opening, you will likely be on high alert.
Associative Learning
Associative memories are the types of information that we learn through traditional Pavlovian
conditioning. For example, recall the classic Nobel prize-winning experiment in physiology
conducted by Ivan Pavlov in the late 1800s. Normally, the presentation of dog food, an unconditioned
stimulus (US), causes a dog to salivate, a naturally occurring behavior, called the unconditioned
response (UR). Dogs are not particularly interested in the sound of a bell: this neutral stimulus will
produce a minor response, such as a head turn and attentional shift towards the origin of the sound,
but not much more than that. However, when this stimulus is repeatedly paired with the presentation
of food, dogs quickly learn to associate that the bell signals food. After multiple pairings, upon
hearing the bell, a conditioned stimulus (CS), the dogs begin to salivate, a conditioned response (CR),
independent of any food being presented.
Figure 66.2.
Associative
Learning. Initially,
in the presence
of an
unconditioned
stimulus (food)
the dog will
salivate, however
in the presence
of a neutral
stimulus (a
ringing bell), the
dog will not
salivate. During
conditioning the
unconditioned
stimulus is paired
with the bell,
causing the dog
to salivate.
Following
conditioning, the
dog will salivate
from only the
presence of only
the bell.
Associative learning can also be measured with instrumental conditioning where a rodent, for
example, learns to associate a response with a meaningful stimulus. For example, a rodent will learn to
press a lever for access to food.
Figure 66.3.
Instrumental
conditioning. In
instrumental
conditioning a
rodent can learn
to associate
pressing a lever
with a
meaningful
stimulus, such as
access to food.
Working Memory
Separate from declarative or procedural memories, a different form of memory called working
memory involves processes of storing information temporarily while simultaneously manipulating
those pieces of information. This type of memory can be thought of as a “short-term memory on
overdrive.” For example, a test of working memory is the digit span test, where a person is given
a series of numbers to remember, then they are asked to repeat the numbers in reverse order. After
successfully completing this task, a different series of numbers, this time one digit longer, is presented
to the patient until they first start making errors in recall. A related task is called the Corsi block
tapping test, where an experimenter sets up several blocks on a table. The experimenter then taps a
series of blocks in a specific order, then the subject is asked to tap on the blocks in reverse order. As with
the digit-span test, the experimenter then makes the series of blocks longer until they make mistakes in
the tapping.
Brain Structures in Memory
Declarative Memory- Cortex

In the 1950’s, the scientist Karl Lashley was interested in finding a location in the brain where
memories were stored. His experiments searched for the location within the cortex of what he called an
“engram” or memory trace. He trained rats to run in a complex maze to reach a food reward, which
took the animals time to learn. After the animals had learned how to successfully run the maze, Lashley
would lesion different areas of the rat cortex and then test the rats in the maze again. He found that
regardless of the location of the lesion, which was done at different areas of the cortex for each rat, that
cortical lesions resulted in decreased ability to remember the location of the food reward and run the
maze. Instead of finding a specific location where the memories were being stored, Lashley discovered
that the size of the lesion corresponded to the amount of memory deficit in the animal, but that the
location of the lesion did not have an effect, demonstrating that the engram was widely spread across
the cortex.
Memory Consolidation and Spatial Memory- Medial

Temporal Lobes, Including the Hippocampus
The main structures of the medial temporal lobes include the hippocampus, the entorhinal cortex,
the perirhinal cortex, and parahippocampal cortex. Together these structures are important in
both consolidation and storage of declarative memories.
The hippocampus, meaning “seahorse” in Greek, was named based on its morphology and
resemblance to a seahorse. The hippocampus is located along the ventral and medial surface of the
brain. The hippocampus is one of the critical structures of the limbic system, a series of subcortical
brain structures that are involved in several different complex behaviors, such as emotions and memory.
The limbic system is an evolutionarily ancient brain network.
The hippocampus is involved in spatial memories, memories involved in navigation of our
surroundings and the creation of a mental map of our world. Spatial memories are developed when
we enter a new building for the first time, and we search for a new classroom. We also use our spatial
memory whenever we are walking around campus, making our way from one building to another,
thinking about the streets you’d need to cross or the buildings you can cut through. While the volume
of the hippocampus is not a reliable indicator of the strength of a healthy person’s spatial memory,
injury to the hippocampus causes deficits in spatial memory.
Patient HM
One of the most influential case studies in the neuroscience of memory is the story of Patient HM.
Since his death in 2008, we now know that Patient HM’s name was Henry Molaison. HM was born
in 1926 in a small Connecticut town. In his childhood, HM began having severe seizures, possibly
the result of a head injury. In his teenage years, he started having tonic-clonic seizures, the most severe
form of seizures that produces a loss of consciousness and convulsions (extreme muscle contraction
or extension). In his early adulthood, he was having a tonic-clonic seizure monthly and several minor
seizures daily, preventing him from working a normal job or living a normal life, despite taking a
cocktail of anti-epileptic medications.
Neurosurgeon William Scoville proposed a “frankly experimental operation” to treat HM. It was
known that most epilepsy originates in patches of neurons of the medial temporal lobe, and HM’s
epilepsy was typical in this respect. Scoville suggested to surgically resect, or remove, the medial
temporal lobe. In 1953, Scoville removed about 8 cm of the medial temporal lobe bilaterally, including
part of the amygdala, and notably the hippocampus, the seahorse-shaped structure of the brain. The
surgery succeeded at its primary goal: HM’s seizures were less frequent and less severe. However, HM
was left with a highly unusual and life-altering side effect: He was unable to create new declarative
memories, a memory deficit called anterograde amnesia. For example, he could not remember what
he had eaten for lunch just minutes after finishing the last bite. Despite being an avid fan of watching
the news, HM couldn’t remember the names or the faces of different celebrities or public figures. It
was as if he was permanently living in the present. (In contrast, retrograde amnesia affects the ability
to successfully retrieve memory from one’s past.)
However, despite his pervasive memory deficits, HM did not display any deficits in intelligence. His
language and speech were unaffected, and word recall was excellent, as he loved completing crossword
puzzles and often did so successfully late in life, with only the occasional spelling errors. He could
learn to acquire new skills, such as keeping a pen still on a moving circular platform, or a tapping task
(these skills are different form of memory called procedural memory). He was also capable of recalling
things from his early childhood, such as geography facts he had learned in elementary school.
The fact that HM’s medial temporal lobe surgery disrupted some types of memories (e.g., memory
for facts) while others were still intact (e.g., motor skills) inspired neuropsychiatrists to try to define the
different forms of memory. Much of the research was led by Dr. Brenda Milner, who carried out several
behavioral tests on HM to figure out what types of memories are dependent on the intact medial
temporal lobe and which ones can function without the medial temporal lobe.
Several tests concluded that HM had lost his ability to create new semantic memories. In one such
study, HM was asked to determine if a word was made up or real. He was shown words with very old
origins, such as “shepherd” or “butcher.” On these words, he performed as well as the control group.
When he was shown words that are made up, such as “phlage” or “thweise”, he likewise performed
as well as the controls. However, when shown words that were added into the dictionary after his
1953-surgery, such as “granola” or “jacuzzi,” he scored about 50% correct – consistent with guessing at
random, as if he never acquired the knowledge that these words have a meaning.
HM was also unable to create autobiographical memories. When asked to recall one of his birthday
celebrations as an adult, he wouldn’t be able to give any significant details about the event. Instead,
his answers were often vague and generic. One interesting observation was that HM’s memory about
details from his childhood were still intact. The inability to recall memories from the past, in this
case, from before HM´s surgery, is called retrograde amnesia. Patient HM’s retrograde amnesia was
temporally graded, meaning that the farther back you examine, the more complete his memories were.
Many of his memories for the two years before his surgery were completely lost, but memories from
his youth and teenage years were intact as much as healthy individuals (there is contention about this
observation, because HM was taking several anti-epileptic drugs, which may have impacted memory
formation.)
From this observation, memory researchers concluded that the medial temporal lobe functions as
short-term storage site for memories, but after some years, those memories get relocated to other brain
areas outside of the medial temporal lobe. Currently, the scientific evidence suggests that memories are
distributed across several networks of cortical and subcortical brain areas.
While HM lost the ability to create new declarative memories, he was still able to maintain a different
class of memories, called procedural memories (or implicit memories). The original test of procedural
memory conducted by Dr. Brenda Milner was called the mirror tracing task. In this test, the patient is
told to draw a third star in between the two stars as quickly as possible without making any mistakes.
The challenge is that the tracing is to be done while watching their hand and the star in their reflection
in a mirror. Because of these unusual circumstances, completing this task is difficult. But over multiple
days of practice, people become better at this mirror tracing task, completing it faster with fewer errors.
Improvement on this task indicates that a person is learning or gaining some memory about how to
better perform the task. After practicing this mirror tracing task, HM was able to finish drawing the
star about ten times faster than when he first began. He improved his performance within each day’s
worth of training, and he also improved day-to-day. There is evidence that he maintained these skills
up to one year later, despite not having regular training on this task.
Surprisingly, each day Milner examined HM, she would need to reintroduce herself since he forgot
who she was. She also had to re-explain what HM was supposed to do in the mirror tracing task. Hence,
while HM was unable to form declarative memory about the experiment or the people involved,
learning of the procedural memories and motor actions involved in this task remained intact.
Based on the deficits seen in Patient HM and other experimental manipulations of the
hippocampus, we conclude that the hippocampus is strongly implicated in the process of declarative
memories and spatial navigation. Since some of HM’s memory functions were still intact, such as
procedural memories and working memory, it is believed that these functions are independent of
hippocampus function.
Tests of Hippocampal Function in Rodents
To test spatial memory behaviorally in nonhuman animals, one test that is regularly used in rodents
is called the Morris water maze. In this test, a shallow pool is filled with an opaque liquid, making it
difficult to see through. Hidden somewhere in this pool is a clear plexiglass platform, and surrounding
the pool are different environmental cues that can be seen from the surface of the water, such as
different shapes or colors. The water is deep enough that when a rodent is put into the Morris water
maze, they have to swim to stay afloat. The rodents swim around aimlessly until they find the platform,
the time it takes for this to happen is recorded, and the trial ends. Over time, the animals learn that the
platform is located near certain navigational cues, and on future trials, the animals spend more time
near those cues, and the latency to find the platform decreases. When the hippocampus is surgically
removed from rodents or inactivated, they perform poorly in the Morris water maze.
Figure 66.4.
Morris water
maze. The Morris
water maze is
used to test
spatial memory
in rodents. A tub
of water is filled
with an opaque
liquid that the
rodent cannot
see through.
Hidden within
the pool is a clear
plexiglass
platform. A
camera is placed
above the pool to
track the rodent’s
path through the
pool. When the
rodent is put in
the pool, it has to
swim around
looking for an
escape. Visual
cues are placed
around the pool
that can be seen
from the surface
of the water,
such as different
shapes or colors.
During a trial, the
time it takes for
the rodent to
find the hidden
platform is
recorded. Over
time and
additional trials,
the animals learn
that the platform
is located near
certain
navigational
cues, and on
future trials, the
animals spend
more time near
those cues,
decreasing the
amount of time it
takes for the
animal to find
the platform.
Another non-human behavioral test used to assess the capacity for learning navigational cues is the
radial arm maze. In this test, a rodent is placed on a circular platform. Extending from this platform
are eight or more “arms”, at the end of each is a small dish. In one of the dishes is a morsel of
food (“rewarded arm”), while the other dishes contain nothing (“non-rewarded arms”). The maze is
designed so that the food cannot be seen from the end of each arm, so the animal must return to the
starting platform before exploring another arm. The number of entries into a non-rewarded arms is
counted as an error. Over time, the animals make fewer errors as they learn which arm is rewarded and
which ones are not. Alzheimer’s disease model organisms perform poorly on this task.
Figure 66.5.
Radial arm maze.
A radial arm
maze is a test of
hippocampal
function. A
central platform
is surrounded by
several arms
radiating away
from the center.
At the end of one
arm is a small
dish that
contains food,
but the other
arms do not have
food. The rodent
must explore the
arm to discover if
it has food or
not. When an
animal travels
down a
non-rewarded
arm it is counted
as an error.
Animals with a
damaged
hippocampus
commit more
errors in this task
than animals
with an intact
hippocampus.
Special populations of neurons
An individual memory is likely distributed widely across several different parts of the brain. However,
there are a few special populations of neurons mainly in the medial temporal lobe that contribute to
highly specific types of memories.
Place cells
Place cells are a special population of pyramidal cells of the hippocampus. These neurons increase
their firing activity when the animal is in a particular location in an environment, indicating that
they contribute strongly to location and navigational memory. There is no apparent topographical
arrangement of these place cells, meaning that adjacent areas of an environment do not necessarily
activate adjacent hippocampal place cells. The place cells, when firing at the right times, help the animal
create a spatial map of their surroundings.
Figure 66.6. Place

Cells. Place cells,
a special
population of
neurons located
within the
hippocampus,
fire action
potentials when
an animal is in a
specific location
and are believed
to be important
in navigational
memory. Here a
rat is placed in a
maze and place
cells within the
hippocampus are
being recorded.
Where each
place cell fires is
indicated on the
maze with
colored dots. All
dots of the same
color indicate
that the same
place cell is firing.
Grid cells
Grid cells are located in the entorhinal cortex, the main input structure to the hippocampus. Closely
related to the place cells described above, grid cells increase their firing properties periodically when
an animal is at an intersection of a “grid” in a wide-open, previously-explored environment. The grid
itself is roughly hexagonal, and spans the whole environment an animal is in. The overlap of multiple
grids gives the animal an idea of the surroundings. The scientific description of grid cells earned three
scientists, Edvard Moser, May-Britt Moser, and John O’Keefe, a Nobel Prize in Physiology or Medicine
in 2014.
Figure 66.7. Grid

cells are a special
population of
neurons located
in the entorhinal
cortex, which
serves as the
main input
structure to the
hippocampus.
Similar to place
cells, grid cells
fire when an
animal is at an
intersection of a
“grid” in a
wide-open,
previously-explor
ed environment.
These images on
the left show the
movement of a
rat through a
square
environment
depicted by a
black line. The
red dots indicate
locations within
the environment
where a specific
entorhinal grid
cell fired. The
images on the
right show a
spatial
autocorrelogram
that corresponds
to the neuronal
activity of the
grid cell from the
figures on the
left.
Concept cells
Concept cells are a series of cortical neurons in the temporal lobe that increase their firing exclusively
in response to highly-specific stimuli, such as the idea of Jennifer Aniston, Halle Berry, or the Tower
of Pisa. These concept cells respond to much more than just pictures: for example, a Luke Skywalker
neuron that responded to a picture of young Mark Hamill (the actor who played Luke Skywalker)
will also respond to text that reads “LUKE SKYWALKER” and the sound of a person saying “Luke
Skywalker”. Although this particular neuron probably won’t fire in response to pictures of athlete
Manu Ginobili or actress Marilyn Monroe, the neuron might fire in response to pictures of Yoda or
Darth Vader, indicating that the neuron may encode an even broader concept, such as “Star Wars
characters” or “Jedi”, or is a part of a network that encodes concepts related to the Star Wars franchise.
Working Memory- Prefrontal Cortex

As part of the frontal lobe, the prefrontal cortex is involved in higher order decision making and
personality. In the context of memory, neural circuits in the prefrontal cortex are important for short-
term and working memory.
A delayed-response task relies on prefrontal cortex activity. In this task, a monkey is shown a cue
of food placed into a randomly selected compartment outside their cage. A screen is then lowered
the compartments are covered for a standard period of time. The screen is then raised again and the
monkey is tasked with correctly uncovering the compartment that contains the food. Animals with a
lesion to the prefrontal cortex perform worse than animals with an intact prefrontal cortex in this task.
Patients with injuries to their prefrontal cortex after stroke, tumors or aneurysm, performed worse
on a variety of working memory tasks such as the digit span test. Additionally, people with
frontotemporal dementia, a neurodegenerative disorder characterized by a degradation of the frontal
lobe, often have difficulty with working memory. The prefrontal cortex also has strong projections
with the hippocampus, and these circuits are likely also involved in the formation of hippocampal-
dependent memories.
Figure 66.8.
Prefrontal
Cortex. The
prefrontal cortex,
shown in blue in
an external view
of the brain, is
located in the
anterior portion
of the frontal
lobe.
Habit or Procedural Memory- Striatum

The striatum, made up of the caudate nucleus and putamen, is important for habit or procedural
memory and reward in learning. A T-maze is used to test procedural learning. In this task, a rodent is
placed at the area of the maze that corresponds to the bottom of the T. The animal is trained to turn
either left (with a high frequency tone) or right (with a low frequency tone once they reach the cross
of the T. If the animal completes the task successfully, they are rewarded with a treat. The ability of the
animal to learn this task is dependent on activity within the striatum. Animals that have striatal lesions
have impaired performance on the T-maze, but not on tasks that rely on other types of memory.
Figure 66.9. T
Maze. A T maze
is used to test
habit, or
procedural
memory and
reward in
learning. In this
task a rodent is
placed at the
area of the maze
that corresponds
to the bottom of
the T. The animal
is trained to turn
either left or
right. If the
animal completes
the task
successfully, they
are rewarded
with a treat.
Cellular Memory
In the late 1800s, around the time when Golgi and Ramon y Cajal were engaged in intense debate
about the organization of the nervous system, many neuroscientists came to a strange observation: the
weight of the brain increases dramatically over the first 10 years of life, but not much more after that.
Even though we learn lots of new facts and make lots of new memories in adulthood, the brain itself
doesn’t grow in size. So how is it possible to store new knowledge if the brain is not making many new
neurons? Most likely, new pieces of information are held in the connections between cells, not just in
the cells themselves. If our estimate of 150 trillion synapses per adult brain is correct, then it is possible
that we could store all the knowledge and memories that we collect over our lifetime through some
combination of activity across certain connections.
The activity at the cellular level is believed to take place on at least three different levels enabling us
to build, store, and retrieve memories.
1. Memory Encoding
Encoding refers to the ability for brain circuits to store some piece of information. In real life, you
are presented with countless stimuli simultaneously. Imagine walking down a busy street, and think
of the number of different sights, smells, and tactile stimuli you experience. Storing memories is
an energetically costly process, and we are limited in the fact that all of our sensory inputs cannot
possibly get encoded. Instead, evolution has preferred to encode stimuli that are most salient pieces of
information, such as perceptual cues associated with predators. Alternatively, information that we pay
strong attention to can get encoded more strongly, like when we repeat a phone number to ourselves
until we have a chance to write it down. It is also easier to encode novel information that “builds” on
previous bits of knowledge, or information that is closely related to other well-established information,
which is why analogies are such an effective way to learn new facts.
2. Memory Consolidation
The process that enables memory storing is called consolidation, which makes the memory more
permanent. In 1949, a student of Karl Lashley, the neuropsychologist Donald O. Hebb, offered an
explanation for how changes in synapses could possibly lead to a phenomenon as complex as learning.
His theory, published in his text The Organization of Behavior, can be summed up in the phrase:
“Cells that fire together, wire together.” In Hebb’s framework, repeated activity at a synapse within a
circuit of neurons acts as a reinforcer signal that strengthens this synapse for future communication,
making the next incoming signal more robust. Hebb also implies the inverse is true: when cells do
not fire together, they weaken their connection. Through fine tuning of synaptic connections, some
strengthening and others weakening, a lifetime of memories can be stored across a wide distribution of
neurons. After a memory has been created, the specific circuit of neurons that represent that piece of
information is called a memory trace or an engram. A cellular process called reverberation is thought to
be the mechanism that allows for consolidation.
Reverberation is the process by which networks of neurons fire repeatedly. Each time that circuit
is activated, the strength of the network is increased, meaning that it becomes easier for that circuit
to be activated in the future. Throughout the process of consolidation, memory traces are thought to
become more represented in the neocortex and less in the subcortical structures like hippocampus or
amygdala.
Recall Patient HM’s temporally graded retrograde amnesia, where he had lost declarative
memories in the two years leading up to this surgery, and yet his memories from the long past were
maintained. This finding suggests that some aspects of declarative memory consolidation depends
at least partially on medial temporal lobe and hippocampus for a period of time, maybe up to two
years, before those memories get stored in the cortex more permanently. However, we cannot conclude
how long consolidation takes in healthy humans based on findings from HM, who had pervasive and
frequent seizures, which might have negatively impacted memory consolidation before the surgery.
Consolidation seems to occur predominantly during sleep. Specifically, declarative memory is
enhanced during non-REM sleep, while procedural memory is enhanced during REM sleep. Studies
investigating sleep consolidation are often done by letting participants learn or perform behavioral
tasks, after which they are deprived of a specific phase of sleep. To wake participants at the right
moment, researchers commonly use EEG signatures, which are unique for different phases of sleep:
sleep deprivation early in the night denies non-REM slow wave sleep, while late-sleep deprivation
decreases time spent in REM sleep. Some speculate that the hallucinations we sometimes experience
during dreaming are a consequence of consolidation processes, but it is inconclusive as to what role
dreaming plays in memory formation.
3. Memory Retrieval
Finally, for the stored memories to be recalled, a cellular process called retrieval happens which
brings back the specific engram. Retrieval happens for both declarative and procedural memories.
The writing of memories can be differentiated from the retrieval of memories using specific priming-
related behavioral tests. One example is a vocabulary recall test. Consider if you were given a list
of 50 words to memorize, words that belong to a handful of different conceptual categories (such as
cinnamon, pepper, or curry, which fall under the category “food flavors”). When asked to write down
as many words as possible from the list using your memory, a test called free-recall, you may be able to
successfully remember about a third of them. However, if you were prompted with the category titles,
a related test called cued-recall, you would perform much better at retrieving those memories, possibly
recalling up to 75% of the words. The fact that cued-recall scores are often higher than free-recall scores
indicate that there is a distinction between the encoding / consolidation of memories and the retrieval
of memories.
Retrieval is not a passive function. When an engram is retrieved, it is reconsolidated, which is
an act similar to replaying the activity of the circuit. During this reconsolidation, it is possible that
some aspects of the memory are emphasized, while others are lost. This is likely the main reason why
we experience false memories, memories that are not true to reality – one reason why eyewitness
testimonies are notoriously unreliable. We may imagine our good memories as better than they actually
were, while simultaneously dampening the negative aspects of those memories. A dysregulation of this
reconsolidation process could lead to the symptoms seen in post-traumatic stress disorder, where the
negative emotional components of a particular memory are exaggerated rather than being blunted.
Figure 66.10.
Memory
encoding and
retrieval. Short
term memory
goes through the
process of
encoding to be
converted into
long term
memory.
Retrieval brings
back a specific
engram for both
declarative and
procedural
memories.
Key Takeaways
• Memory can be divided into declarative memory, nondeclarative memory, and

working memory. Declarative memory can be further subdivided into semantic
memory and episodic memory.
• The cortex, hippocampus, striatum, and prefrontal cortex are all structures with
specific roles in memory.
Attributions
Media Attributions
• Types of memory © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

• classical conditioning © Salehi.s adapted by Valerie Hedges is licensed under a CC BY-SA
• instrumental conditioning © DataBase Center for Life Science (DBCLS) adapted by Valerie
Hedges is licensed under a CC BY (Attribution) license
• Morris water maze © Samueljohn.de adapted by Valerie Hedges is licensed under a CC BY-SA
• radial arm maze © Mcole13 adapted by Valerie Hedges is licensed under a CC BY-SA
• Place cells © Stuartlayton adapted by Valerie Hedges is licensed under a CC BY-SA (Attribution
ShareAlike) license
• Grid cells © Lisa M. Giocomo, May-Britt Moser, Edvard I. Moser, adapted by Valerie Hedges is
licensed under a CC BY-SA (Attribution ShareAlike) license
• PrefrontalCortex-1024×472-1 © Casey Henley adapted by Valerie Hedges is licensed under a
• T maze © ataBase Center for Life Science (DBCLS) adapted by Valerie Hedges is licensed under
a CC BY (Attribution) license
• Encoding_and_retrieval_image © Mcmactavish adapted by Valerie Hedges is licensed under a
CC BY-SA (Attribution ShareAlike) license
920 | MOLECULAR MECHANISMS OF MEMORY: APLYSIA
67.
MOLECULAR MECHANISMS OF
MEMORY: APLYSIA
Through the 1960s, psychiatrist Eric Kandel and his

colleagues Arvid Carlsson and Paul Greengard worked
with the marine mollusk Aplysia californica. Their work
Resources uncovering the neural mechanisms of learning and
memory earned them the Nobel Prize in Physiology or
Medicine in 2000.
With a nervous system of only 20,000 cells, Aplysia is
• Glossary terms orders of magnitude simpler than the other model
• Scientist links to learn organisms used at the time. Additionally, some Aplysia
more! neurons are huge, up to a millimeter in diameter, which
• Key Takeaways took away the need for highly precise equipment.
Figure 67.1.
Aplysia
californica.
Picture of an
Aplysia
californica
specimen.
Aplysia also has a relatively simple anatomy. It breathes using a half-circle of delicate tissue called the
gill, which is guarded by the mantle shelf. They also have an organ called the siphon, a small tube
that is used for moving water through the animal.
MOLECULAR MECHANISMS OF MEMORY: APLYSIA | 921
Figure 67.2
Aplysia anatomy.
Aplysia is a sea
slug that has
anatomical
features that
have been
utilized by
neuroscientists
to learn more
about the
processes of
learning and
memory. The
Aplysia uses their
delicate gill to
breath, which is
protected by the
mantle shelf. The
siphon is a small
tube that is used
to move water
through the
animal and the
tail helps the
animal move in
the water.
Kandel and his colleagues began their exploration of memory by studying the gill-withdrawal reflex,
a defensive motor response behavior. When a stimulus, such as a hungry predator (or an experimenter’s
paintbrush), grazed the siphon, the Aplysia would reflexively withdraw their gill, as if to protect this
vital organ by shrinking away from the threat. However, after repeated brush strokes to the siphon,
the sea slugs figured out that the stimulus was completely innocuous and decreased the strength of gill
withdrawal. Kandel and team suggested that this change in behavior was a form of learning. Kandel
and colleagues used Aplysia to learn about the processes of habituation and sensitization.
Habituation
The gill-withdrawal reflex circuit relies heavily on two different populations of neurons: the
glutamatergic sensory neurons that receive somatosensory information from the skin of the siphon,
and the motor neurons that control the muscles of the gill. By using two different tiny glass pipettes,
they could impale these neurons, inducing action potential firing in the sensory neuron, and observe
changes in membrane potential of the motor neurons. Activation of the sensory neuron causes release
of glutamate onto the motor neuron that controls the gill, binding to glutamatergic receptors and
causing an EPSP response in the motor neuron.
Figure 67.3.
Synapse
between siphon
sensory neuron
and gill motor
neuron. The
siphon sensory
neuron is
glutamatergic,
which results in
the release of
glutamate onto
the motor
neuron that
serves the gill
when the siphon
is touched with a
paintbrush.
When glutamate
binds to the
motor neuron it
causes excitation
of the motor
neuron and
contraction of
the gill.
Although there was a strong gill withdrawal reflex following the one poke, Kandel and colleagues
observed that after repeated pokes to the siphon, the gill withdrawal reflex got weaker. They found
that the activity of the siphon neuron remained unchanged, however, the activity of the motor neuron
supplying the gill decreased. The reflex had been habituated. This habituation was due to a decreased
release of glutamate from the siphon sensory neuron onto the gill motor neuron.
Sensitization
The group went about seeing if they could modify this habituated response, curious if a stored
memory can be modified by stimuli from the outside world. When they paired the mild siphon touch
with a painful electric shock to the tail, the Aplysia began responding with a strong motor reaction,
withdrawing the gill very intensely, indicating that the inhibited response disappeared. They called this
observation sensitization. In electrophysiological studies, they observed that the EPSP at the motor
neuron was much larger following the tail shock.
On a molecular level, presentation of sensitization is downstream of the action of a third population
of neurons, serotonergic interneurons that synapse onto the siphon sensory neurons. The noxious
stimulus triggers these interneurons to release the neurotransmitter serotonin, which binds to
receptors on the terminals of the siphon sensory neurons, increasing adenylyl cyclase activity and
cAMP production. cAMP activates protein kinase A, which in turn increases the release of glutamate
from the sensory neuron and strengthening the gill withdrawal reflex.
Figure 67.4.
Shocking the tail
produces
sensitization of
the gill
withdrawal
reflex. After
producing a
habituated
response by
touching the
siphon
repeatedly, a tail
shock was
provided, which
results in the
return of a strong
gill withdrawal
reflex. Shocking
the tail excites a
serotonergic
neuron that
synapses on the
siphon sensory
neuron. This
facilitates the
release of
glutamate from
the siphon
sensory neuron
onto the gill
motor neuron.
Repeating the sensitization protocol multiple times results in plastic changes at the synapse between
the sensory siphon neuron and the motor neuron. These plastic changes are long-lasting and
dependent on new gene expression. The activated protein kinase A can translocate to the siphon
sensory neuron nucleus to activate gene expression and the production of proteins that can remodel
the anatomy of the synapse through the growth of additional sensory neuron axons, increasing
synaptic connections.
Key Takeaways
• Aplysia is a simple animal model that has been used to study learning and memory.
• Touching the siphon activates the gill withdrawal reflex through the release of
glutamate on to the gill motor neuron.
• Repeatedly touching the siphon results in habituation of the gill withdrawal reflex,
and a weaker gill withdrawal.
• Shocking the tail following habituation activates a serotonergic neuron that
facilitates glutamate release from from the siphon neuron, producing a strong gill
withdrawal reflex.
• Over the long-term, this produces long lasting plasticity within this synapse.
Attributions
Media Attributions
• aplysia © Columbia University, New York adapted by Valerie Hedges is licensed under a Public
Domain license
• aplysia anatomy © Phillip Henry Gosse adapted by Valerie Hedges is licensed under a Public
Domain license
• Synapse between the siphon and the gill © Valerie Hedges is licensed under a CC BY-NC-SA

• Synapse between siphon, tail and gill © Valerie Hedges is licensed under a CC BY-NC-SA
MOLECULAR MECHANISMS OF MEMORY: HIPPOCAMPUS | 927
68.
MOLECULAR MECHANISMS OF
MEMORY: HIPPOCAMPUS
Zooming in beyond the level of anatomy, the substrates of

learning can be found at the level of synapses. Synapses
change in a phenomenon called plasticity. The word
“plasticity” refers to a change in synaptic strength, which Resources
may be an increase or a decrease. This change may persist
for minutes, hours, days, or in some cases, even a whole
lifetime.
When synaptic strength is increased and remains • Glossary terms
elevated, we call this long-term potentiation (LTP). A • Key Takeaways
prolonged weakness of a synapse is called long-term • Scientist links to learn
depression (LTD). In our current limited understanding more!
of plasticity, both phenomena are important for a healthy
brain, and neither one is always good or always bad. It is
also important to clarify that both excitatory synapses and
inhibitory synapses can be subject to either LTP or LTD.
The Hippocampus
The hippocampus, meaning “seahorse” in Greek, was named based on its morphology. It is located
along the ventral and medial surface of the brain. The hippocampus serves as one of the critical
structures of the limbic system, a series of subcortical brain structures that are involved in several
different complex behaviors, such as emotions and memory. The synaptic connectivity of the
hippocampus is very well characterized. Hippocampal synaptic connectivity was first described by
928 | MOLECULAR MECHANISMS OF MEMORY: HIPPOCAMPUS
Ramon y Cajal, and is made up of three main synaptic connections; sometimes called the trisynaptic
circuit.
First, the entorhinal cortex serves as the major input to the hippocampus. This white matter
signaling tract is called the perforant pathway, and the neurons synapse onto the granule cells within
an area of the hippocampus called the dentate gyrus. The dentate gyrus neurons send their axons,
called mossy fibers, to the pyramidal cells of the CA3 region of the hippocampus. The CA3 neurons
have axonal projections called Schaffer collaterals that project out of the hippocampus via the fornix
and also to neurons within an area of the hippocampus called CA1, which are also neurons that serve
as an output of the hippocampus.
Figure 68.1.
Rodent
hippocampal
trisynaptic circuit.
Neurons from
the entorhinal
cortex serve as
the input to the
hippocampus.
The axons of
these cells form
the perforant
path (orange).
The entorhinal
cortex neurons
synapse onto
granule cells in
the dentate
gyrus (red). The
dentate gyrus
cells extend their
axons, called
mossy fibers, to
cells in a region
of the
hippocampus
called CA3. The
CA3 neurons
(purple) extend
axons, called
Shaffer
collaterals, to
another area of
the hippocampus
called the CA1
region (blue).
‘Rodent
hippocampal
trisynaptic circuit’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Long-Term Potentiation (LTP)

Long-term potentiation (LTP) is a long-lasting increase in synaptic strength, measured by the
amplitude of the post-synaptic potential. Plasticity can be measured throughout these connections,
but for our purposes we will examine how to create LTP within the Schaffer collateral.
To create LTP within the Schaffer collateral, a brief electrical stimulus must be provided to the
presynaptic axons coming from the CA3 cells via a stimulating electrode. The EPSP generated in the
postsynaptic CA1 neurons is then measured with a recording electrode to establish a baseline.
Figure 68.2.
Inducing Schaffer
collateral LTP. To
induce LTP
through the
Schaffer
collateral, a
stimulating
electrode is
positioned to
electrically excite
the Schaffer
collaterals, which
are also the axon
terminal of the
CA3 neurons. A
recording
electrode is
placed
postsynaptically
in the CA1
neurons. First,
baseline
measurements
are taken by
providing brief
stimulation to
the CA3 neurons
and recording the
amplitude of the
EPSP
postsynaptic
response. After
establishing a
baseline, a
tetanus is
delivered the the
CA3 neuron,
consisting of
short burst of
high frequency
stimulation.
Following the
delivery of the
tetanus, a single
brief stimulation
is provided to the
CA3 neuron and
the postsynaptic
response in the
CA1 neuron is
measured. An
increased
amplitude of the
EPSP in the CA1
neuron indicates
LTP. ‘Inducing
Schaffer
collateral LTP’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Using Hebb’s theory about plasticity, if “cells that fire together, wire together,” then it stands that
repeated stimulation of that synapse would induce a rewiring of the connection, resulting in LTP.
To induce LTP within these cells, a tetanus, or a very intense electrical stimulation consisting of 100
stimulations a second (100 Hz) is delivered to the presynaptic CA3 cells for 3 seconds. Following
the delivery of tetanus, a single stimulus is provided again and the EPSP is then measured in the
postsynaptic CA1 neuron. The delivery of the high frequency stimulation results in an increased
amplitude of the postsynaptic EPSP in response to a single stimulus, demonstrating that LTP is a
measurable phenomenon.
Figure 68.3.
Graphing LTP. In
this graph the
magnitude of the
postsynaptic
EPSP is being
measured on the
y-axis over time.
After establishing
a baseline, a high
frequency
stimulation
(tetanus) is
delivered. After
the high
frequency
stimulation, the
magnitude of the
postsynaptic
response
increases and
remains elevated
for a long-period
of time,
indicating LTP.
‘Graphing LTP’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
In many cases LTP is shown graphically by measuring the EPSP amplitude as a percent of the control
EPSP amplitude. Prior to tetanus delivery, the amplitude will be measured as 100%, or the same as
control. Following tetanus, the amplitude of the EPSP will be larger than it was baseline, for instance
130% indicating a 30% increase in amplitude. Increasing the amplitude of the postsynaptic EPSP
increases the likelihood of a neuron firing an action potential by increasing the neuron membrane
potential such that it is closer to the threshold potential for the cell.
It has been demonstrated in rodents that the elevated postsynaptic EPSP response is long-lasting and
can remain elevated for upwards of one year. In humans, we theorize that some synaptic connections
may remain potentiated for our entire lifetime, however investigating this is in humans is ethically
constrained.
Figure 68.4.
Graphing
postsynaptic
response as a
percent of
control. Here, the
postsynaptic
EPSP amplitude
is being
measured as a
percent of the
control
measurements.
Prior to the high
frequency
stimulation, the
EPSP amplitude
is 100%.
Following the
high frequency
stimulation, the
EPSP amplitude
is increased
above the
control,
indicating that
there is a larger
postsynaptic
response. In this
example, the
EPSP amplitude
remains elevated
for upwards of
one year of time,
indicating that
LTP can be
long-lasting.
‘Graphing
postsynaptic
response as a
percent of
control’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Glutamate Receptors and LTP

Long-lasting changes in synaptic strength, such as the LTP, are made possible through a series of
molecular and cellular level changes. One form of LTP results from a change in the types of
glutamatergic receptors. Of the three classes of ionotropic glutamate receptors, two are important for
this form of LTP: the AMPA and the NMDA receptors (Chapter 16).
When a molecule of glutamate binds to the active site of the AMPA (α-
amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor, the ligand-gated ion channel changes
to the open conformation and allows the cations, sodium and potassium, to cross the cell membrane.
Sodium moves into the cells more than potassium leaves the cell, leading to depolarization. The
NMDA (N-methyl-D-aspartate) receptor requires the binding of glutamate to open, but it is also
dependent on voltage. The pore of the NMDA ligand-gated ion channel is blocked by a molecule of
magnesium when the membrane potential is below or near resting membrane potential, preventing
ions from moving through the channel. Once the cell depolarizes, the magnesium block is expelled
from the receptor, which allows sodium, potassium, and calcium to cross the membrane.
The voltage change needed to open the NMDA receptor is usually a result of AMPA receptor
activation. Released glutamate binds to both AMPA and NMDA receptors, and sodium influx occurs
through open AMPA channels, which depolarizes the cell enough to expel the magnesium ion and
allow ion flow through the NMDA receptors.
Figure 68.5.
AMPA and
NMDA. (A)
When below the
voltage
threshold,
glutamate binds
to AMPA
receptors,
causing the
ionotropic
receptor to open
and allow
passage of both
sodium and
potassium. The
movement of
sodium into the
cell dominates,
indicated by the
bold arrow.
However, the
binding of
glutamate to the
NMDA receptor
does not allow
the movement of
ions across the
membrane due
to a magnesium
ion blocking the
pore of the
channel. (B)
When the
membrane is
depolarized
above the
threshold voltage
of the NMDA
receptor, the
magnesium ion is
expelled from the
pore of the
channel, allowing
movement of
calcium, sodium,
and potassium
through the
NMDA channel.
The movement
of calcium and
sodium into the
cell dominates,
indicated by the
bold arrows.
LTP and Intracellular Calcium

The calcium that enters the cell through the open NMDA receptors activates various kinases
including, protein kinase A (PKA), protein kinase C (PKC) and calcium-calmodulin-dependent
kinase II (CAMKII). Kinases phosphorylate target proteins within the cell, including AMPA
receptors. Phosphorylation of AMPA receptors increases their conductance, allowing more ions to pass
through the receptors and leading to a greater degree of depolarization. Further, increased intracellular
calcium can lead to the insertion of additional AMPA receptors into the postsynaptic cell membrane,
which will also allow more sodium to move into the cell and lead to increased depolarization.
Figure 68.6.
Calcium and LTP.
Calcium enters
the cells through
NMDA receptors.
When
intracellular
calcium levels are
high, the calcium
activates protein
kinases within
the cell. The
protein kinases
phosphorylate
AMPA receptors
expressed on the
cell surface,
increasing their
conductance and
allowing more
sodium to enter
the cell. Kinases
also cause
additional AMPA
receptors to be
inserted into the
membrane of the
cell, thereby
increasing the
available
receptors that
glutamate can
bind. ‘Calcium
and LTP’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Long-Term Depression (LTD)

Long-term potentiation increases the strength of synapses. Changes in activity can also cause synapses
to be weakened through the process of long-term depression (LTD). Again, using the Schaffer
collateral as an example, long-term depression can be induced at the synapses between the CA3
and CA1 neurons by replacing the tetanus stimulation with low-frequency stimulation for longer
periods of time. For instance, a 15 min exposure to 1 Hz stimulation will lead to a decreased EPSP
amplitude.
Through LTP and LTD, synapses demonstrate bidirectional plasticity that is dependent on the type
of stimulation that the synapse receives.
Figure 68.7.
Graphing LTD. In
this graph the
magnitude of the
postsynaptic
EPSP is being
measured on the
y-axis over time.
After establishing
a baseline, a low
frequency
stimulation is
delivered. After
the low
frequency
stimulation, the
magnitude of the
postsynaptic
response
decreases and
remains lowered
for a long-period
of time,
indicating LTD.
‘Graphing LTD’
by Valerie
Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
LTD and Intracellular Calcium

Even with low-frequency stimulation of the synapse, glutamate will bind to post-synaptic NMDA
receptors and allow for the influx of calcium into the cell. Weak depolarization of the membrane
leads to a low amount of calcium entering the cell, which activates a separate class of enzymes call
protein phosphatases, specifically protein phosphatase 1 and protein phosphatase 2, that remove
phosphate groups from target proteins. Further, LTD causes AMPA receptors to be internalized, or
removed from the postsynaptic membrane. Removal of AMPA receptors will decrease excitability.
Figure 68.8.
Calcium and LTD.
Calcium enters
the cells through
NMDA receptors.
When
intracellular
calcium levels are
low, the calcium
activates protein
phosphatases
within the cell.
The protein
phosphatases
de-phosphorylat
e AMPA
receptors
expressed on the
cell surface,
decreasing their
conductance of
sodium. Further,
phosphatases
cause AMPA
receptors to be
internalized and
removed from
the cell surface,
thereby
decreasing the
available
receptors that
glutamate can
bind. ‘Calcium
and LTD’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Relating LTP to Learning

To test how LTP And LTD are directly related to memory, a robust model of learning is used called
Inhibitory Avoidance. In an inhibitory avoidance task, a rat learns to associate an environment with
an aversive experience. Typically, a two chamber compartment is used with one light side and one dark
side. The animal is permitted to move from the light chamber to the dark chamber, where it receives
an electric foot shock. After just one trial with this protocol, the animal will learn to avoid the dark
chamber where it received the foot shock.
One trial of inhibitory avoidance led to measurable LTP within the Schaffer collaterals,
demonstrating that the behavioral learning corresponded to LTP during this task. Administration
of an NMDA receptor blocker to the hippocampus prior to inhibitory avoidance training results in
the animals being unable to learn that the dark chamber leads to a foot shock and inhibition of the
corresponding LTP.
Figure 68.9.
Inhibitory
avoidance. In this
task, rodents
learn to associate
an environment
with an aversive
experience, in
this case a foot
shock. In a two
chamber
compartment
one side is light
and one side is
dark to
distinguish the
two
compartments.
When the animal
is placed in the
dark
compartment it
receives an
electric foot
shock. After just
one trial with this
protocol, the
animal learns to
avoid the dark
chamber where it
received the foot
shock. One trial
of inhibitory
avoidance led to
measurable LTP
within the
Schaffer
collaterals,
demonstrating
that the
behavioral
learning
corresponded to
LTP during this
task. ‘Inhibitory
avoidance’ by
Valerie Hedges is
licensed under a
Creative
Commons
Attribution
Non-Commercial
Share-Alike
(CC-BY-NC-SA)
4.0 International
License.
Key Takeaways
• Plasticity in the form of LTP and LTD occurs at both inhibitory and excitatory
synapses
• The hippocampus has a trisynaptic circuit of connections
• Long-term potentiation (LTP) is a long-term strengthening of a synapse, whereas
long-term depression (LTD) is a long-term weakening of a synapse.
• LTP is demonstrated by observing an increased EPSP amplitude following a tetanus.
• LTD is demonstrated by observing a decreased EPSP amplitude following low
frequency stimulation.
• AMPA and NMDA glutamate receptors are important in LTP.
• High levels of intracellular calcium from high frequency stimulation activates protein
kinases, which leads to increased conductance of AMPA receptors and insertion of
AMPA receptors in the cell membrane.
• Low levels of intracellular calcium from low frequency stimulation activates protein
phosphatases, which leads to decreased conductance of AMPA receptors and
removal of AMPA receptors in the cell membrane.
• Inhibitory avoidance is a robust learning model in which animals only need one trial
before learning occurs. It has been show to also induce LTP.
Attributions
Media Attributions
• Rodent hippocampus trisynaptic circuit © Valerie Hedges is licensed under a CC BY-NC-SA

• Schaffer collateral LTP © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• LTP Graph © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Graphing postsynaptic response as a percent of control © Valerie Hedges is licensed under a CC
• AMPA NMDA © Casey Henley is licensed under a CC BY-NC-SA (Attribution
• Calcium and LTP © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• LTD graph © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution NonCommercial
ShareAlike) license
• Calcium and LTD © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution
• Inhibitory avoidance © Valerie Hedges is licensed under a CC BY-NC-SA (Attribution

ADDITIONAL REVIEW ANSWERS | 949
ADDITIONAL REVIEW ANSWERS
Chapter 1
Questions 1 and 2
The neuron is composed of a number of specialized structures that are critical for proper functioning.
Dendrites branch out from the cell body or soma like a tree. The word dendrite comes from the word
for tree in Greek. The dendrites are the primary input zone for the cell and receive information from
other cells. The number of inputs a neuron receives depends on the complexity of the dendritic
branching. The soma houses the nucleus and most organelles and is the location of gene
transcription, protein synthesis, vesicle packaging, and other cellular machinery mechanisms. The
axon hillock is located at the transition of the soma and axon. It is the location where action potential
propagation begins. The axon transmits electrical signals, called action potentials, from the cell body
to the presynaptic terminal. Some axons are covered by myelin sheath, which is an insulating material
that allows the action potential to travel down the axon faster. Between the myelin segments are the
Nodes of Ranvier where the axonal cell membrane is open to the extracellular space. Ions can flow
across the membrane at the Nodes, which allows for regeneration of the action potential. Finally, the
presynaptic terminal is the output zone. After the action potential reaches the terminal,
neurotransmitters are released into the synapse to send messages onto the next cell.
950 | ADDITIONAL REVIEW ANSWERS
Question 3
Myelin increases the speed at which action potentials flow down the axon. If myelin was lost, action
potentials would propagate down the axon at a slower rate or possibly not at all. Normal sensory,
motor, and cognitive functions would be dysfunctional, depending on the location of the
demyelination.
Chapter 2
Question 1
Ions move from regions of high concentrations to regions of low concentrations by a process called
diffusion. The concentration gradient will cause ions to diffuse across an impermeable membrane if
there are open ion channels. Ions are also attracted to regions of opposite charge, so the electrical
gradient will move cations toward a negative charge and anions toward a positive charge.
Figure 2.4. Concentration and electrical gradients drive ion movement. Ions diffuse down
concentration gradients from regions of high concentration to regions of low concentration. Ions
also move toward regions of opposite electrical charge. ‘Gradients’ by Casey Henley is licensed
under a Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License. View
animation of image.
Question 2
When an ion is at equilibrium, there will be no net ion flow in either direction. The ions will still
move however, there will simply be an equal number of ions moving into the cell than are moving
out.
Figure 2.5. When an ion is at equilibrium, which occurs when the concentration and electrical
across the membrane through open channels, but the ion flow into and out of the cell is equal . In
this animation, the membrane starts and ends with seven positive ions on each side even though
the ions move through the open channels. ‘Ion Equilibrium’ by Casey Henley is licensed under a
Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License. View
animation of image.
Chapter 3
Question 1
The membrane potential is the difference in electrical charge between the inside and outside of a cell.
The extracellular solution is the reference point, so you should think of that as having no charge. If a
cell has a membrane potential of -70 mV, that means the inside of the cell is more negative than the
outside. Active neurons can have drastic changes in their membrane potentials, but the resting
membrane potential is the difference in charge when the cell is not active.
Figure 3.1. The membrane potential is measured using a reference electrode placed in the
extracellular solution and a recording electrode placed in the cell soma. The membrane potential is
the difference in voltage between these two regions. ‘Measuring Membrane Potential’ by Casey
Question 2
The membrane potential is the difference in charge inside the cell relative to the outside, so it takes
into account permeability and concentration of all charged molecules when the cell is at rest. It is
calculated by the Goldman equation. Equilibrium potential is the membrane potential at which one
specific ion is at equilibrium, meaning there is no net movement of that ion in either direction. A cell
would reach the equilibrium potential of an ion if the membrane were permeable to only that ion. It
is calculated by the Nernst equation.
Question 3
Nernst equation:
For Potassium:
z=1
[Ion]inside = 125 mM
[Ion]outside = 15 mM
Chapter 4
Question 1
Goldman equation:
Increase extracellular potassium:
Question 2
Decrease extracellular sodium:
Question 3
A change in potassium’s extracellular concentration had a much larger effect on the resting membrane
potential compared to changed sodium’s extracellular concentration.
Question 4
The reason for this is because the membrane is significantly more permeable to potassium compared
to sodium at rest. Since there are open channels, potassium can diffuse across the membrane and alter
the resting membrane potential. Sodium may increase in concentration outside the cell, but without a
way to cross the membrane, the membrane potential does not change.
Question 5
At rest, the neuronal membrane includes open non-gated potassium channels, fewer open chloride
channels, very few open sodium channels, and sodium-potassium pumps.
Chapter 6
Question 1
Figure 6.3. EPSPs that summate to reach threshold initiate the action potential. The depolarizing
rising phase moves the membrane potential from threshold to above 0 mV. The overshoot is the
peak of the action potential where the membrane potential is positive. The falling phase repolarizes
the membrane potential, and the undershoot takes the membrane potential more negative than the
resting membrane potential. After the undershoot, the membrane potential returns to rest. ‘Action
Potential Phases’ by Casey Henley is licensed under a Creative Commons Attribution
The rising phase is a depolarization. The voltage-gated sodium channels are open. The voltage-gated
potassium channels are closed.
The falling phase is a repolarization. The voltage-gated sodium channels are inactivated. The
voltage-gated potassium channels are open.
The undershoot is a hyperpolarization. The voltage-gated sodium channels are closed. The voltage-
gated potassium channels close during the undershoot.
Question 2
The absolute refractory period occurs when the voltage-gated sodium channels are either open during
the rising phase or inactivated during the falling phase.
The relative refractory period occurs when the voltage-gated sodium channels are de-inactivated
during the undershoot, but the voltage-gated potassium channels are still open.
Question 3
The cell cannot fire a second action potential during the absolute refractory period. The voltage-gated
sodium channels cannot reopen in response to a stimulus, regardless of the strength.
The cell can fire a second action potential during the relative refractory period, but the strength of
the stimulus must be stronger than when the cell is at rest since the membrane potential is
hyperpolarized. The cell will require more stimulus to reach threshold.
Chapter 7
Question 1
In this voltage-clamp experiment, the neuron is clamped at a membrane potential of 0 mV starting a
the 1 msec time point. This depolarization is past threshold, so the voltage-gated sodium channels
open transiently, increasing sodium permeability and allowing sodium ions to rush into the cell,
shown by the inward ion flow on the graph. Briefly after opening, the voltage-gated sodium channels
inactivate, preventing any further inward current. The delayed-rectifier voltage-gated potassium
channels then open, increasing potassium permeability and allowing potassium ions to rush out of
the cell, shown by the outward ion flow on the graph.
Chapter 10
Question 1
1. An action potential arrives in the terminal
2. The depolarization opens voltage-gated calcium channels
3. Calcium interacts with synaptotagmin, a protein bound do synaptic vesicles
4. Synaptotagmin interacts with the SNARE proteins, causing the synaptic vesicle membrane to
fuse with the cellular membrane
5. Neurotransmitters are released into the synaptic cleft via exocytosis.
Chapter 11
Question 1
The postsynaptic cell would show an EPSP
A depolarization above rest at -60 mV.
Question 2
The postsynaptic cell would show an IPSP
A hyperpolarization below rest at -60 mV.

Question 3
With this repetitive firing comes a summation of individual EPSPs, which leads to an increase in
depolarization. Once the depolarization reaches threshold, the cell will fire an action potential, but
the recording electrode in this example is located in the cell body, not the axon, so action potentials
will not be measured.
Summated EPSPs
Chapter 12
Question 1
Neurotransmitters can activate both ionotropic and metabotropic receptors.
Ionotropic receptors are themselves an ion channel, allowing direct ion flow after neurotransmitter
binding. Action is immediate and rapid.
Metabotropic receptors do not form an ion channel, and if they affect ion flow it is done indirectly
via G-proteins. The effects are slower than ionotropic receptors and have broader actions.
Metabotropic receptors can open ion channels, but they can also send second messengers into the cell,
which have downstream cellular effect and can even alter gene transcription and protein translation.
Chapter 19
Question 1
Rods and cones play specialized roles in the perception of sight.
Physical appearance: Rods have an elongated, cylindrical outer segment; cones have a shorter,
conical outer segment.
Light sensitivity: Rods are highly sensitive to light and therefore are specialized for low light levels.
Cones, on the other hand, have lower sensitivity to light, so they are specialized for bright light
conditions.
Location: Rods are almost completely excluded from fovea, but the fovea is the location of the
majority of cones.
Convergence: Rods have high convergence on bipolar cells; cones have low convergence and can have
a 1 to 1 pairing of photoreceptor to bipolar
Question 2
The fovea is the region of greatest vision acuity. It consists mainly of cone photoreceptors.
The optic disc is the region where the ganglion cell axons leave the retina as the optic nerve. It is
responsible for our blindspot because of the absence of photoreceptors in the region.
Chapter 22
Question 1
Different areas of the body have different two-point discrimination thresholds. The more receptors
that innervate an area, the smaller the receptive fields of those receptors, which leads to a smaller two-
point discrimination threshold.
Chapter 23
Question 1
The primary somatosensory cortex is located in the postcentral gyrus – named because it is the gyrus
just posterior to the central sulcus.
Question 2
Neurons traveling from brainstem will synapse in the ventral posterior nucleus of the thalamus before
traveling to the primary somatosensory cortex.
IMAGES OF ANIMATIONS | 963
IMAGES OF ANIMATIONS
Chapter 1
Animation 1
Figure 1.11. The action potential is a brief but significant change in electrical potential across the
964 | IMAGES OF ANIMATIONS
Chapter 2
Animation 1
Figure 2.3. When ion channels in the membrane are closed, ions cannot move into or out of the
neuron. Ions can only cross the cell membrane when the appropriate channel is open. For example,
only sodium can pass through open sodium channels. The dotted, blue channels represent sodium
channels; the striped, green channels represent potassium channels; the solid yellow channels
represent chloride channels. ‘Ion Movement’ by Casey Henley is licensed under a Creative Commons
Attribution Non-Commercial (CC-BY-NC) 4.0 International License.
Animation 2
Figure 2.4. Concentration and electrical gradients drive ion movement. Ions diffuse down
concentration gradients from regions of high concentration to regions of low concentration. Ions
also move toward regions of opposite electrical charge. ‘Gradients’ by Casey Henley is licensed
under a Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License.
Animation 3
Figure 2.5. When an ion is at equilibrium, which occurs when the concentration and electrical
across the membrane through open channels, but the ion flow into and out of the cell is equal . In
this animation, the membrane starts and ends with seven positive ions on each side even though
the ions move through the open channels. ‘Ion Equilibrium’ by Casey Henley is licensed under a
Creative Commons Attribution Non-Commercial (CC-BY-NC) 4.0 International License.
Chapter 3
Animation 1
Figure 3.7. A) At rest, both the concentration and electrical gradients for sodium point into the cell.
As a result, sodium flows in. As sodium enters, the membrane potential of the cell decreases and
becomes more positive. B) As the membrane potential changes, the electrical gradient decreases in
strength, and after the membrane potential passes 0 mV, the electrical gradient will point outward,
since the inside of the cell is more positively charged than the outside. C) The ions will continue to
flow into the cell until equilibrium is reached. An ion will be at equilibrium when its concentration
and electrical gradients are equal in strength and opposite in direction. The membrane potential of
the neuron at which this occurs is the equilibrium potential for that ion. Sodium’s equilibrium
potential is approximately +60 mV. The dotted, blue channels represent sodium channels; the
striped, green channels represent potassium channels; the solid yellow channels represent chloride
channels.‘ Sodium Gradients’ by Casey Henley is licensed under a Creative Commons Attribution
Chapter 4
Animation 1
Figure 4.3. Electrochemical gradients drive potassium out of the cell, removing positive charge,
making the cell’s membrane potential more negative, in the direction of potassium’s equilibrium
potential. The dotted, blue channels represent sodium leak channels; the striped, green channels
represent potassium leak channels; the solid yellow channels represent chloride leak channels.
‘Potassium at Rest’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 2
Figure 4.4. The membrane is most permeable to potassium at rest, and this leads to potassium
efflux. However, the membrane is also permeable to chloride and sodium, and the flow of these
ions keep the resting membrane potential more positive than potassium’s equilibrium potential. The
dotted, blue channels represent sodium leak channels; the striped, green channels represent
potassium leak channels; the solid yellow channels represent chloride leak channels. ‘Ion Flow at
Rest’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 3
Figure 4.5. The sodium-potassium pump is embedded in the cell membrane and uses ATP to move
sodium out of the cell and potassium into the cell, maintaining the electrochemical gradients
necessary for proper neuron functioning. A) Three intracellular sodium ions enter the pump. B) ATP
is converted to ADP, which leads to a conformational change of the protein, closing the intracellular
side and opening the extracellular side. C) The sodium ions leave the pump while two extracellular
potassium ions enter. D) The attached phosphate molecule then leaves, causing the pump to again
open toward the inside of the neuron. E) The potassium ions leave, and the cycle begins again.
‘Sodium-Potassium Pump’ by by Casey Henley is licensed under a Creative Commons Attribution
Chapter 5
Animation 1
Figure 5.3. Top panel: A stimulus can cause ion channels in the membrane of the cell body or
dendrites to open. Bottom panel: The open ion channels allow ion flow across the membrane. The
dotted, blue channels represent sodium channels; the striped, green channels represent potassium
channels; the solid yellow channels represent chloride channels. ‘Postsynaptic Ion Flow’ by Casey
Animation 2
Figure 5.4. When a stimulus opens sodium channels, sodium rushes into the cell because the
equilibrium potential of sodium is +60 mV. This causes an excitatory depolarization called an
excitatory postsynaptic potential (EPSP). After the stimulus, the ion channels close, and the
membrane potential returns to rest. The dotted, blue channels represent sodium channels; the
striped, green channels represent potassium channels; the solid yellow channels represent chloride
channels. ‘EPSP’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 3
Figure 5.5. When a stimulus opens chloride channels, and the resting membrane potential is more
positive than chloride’s equilibrium potential of -65 mV, chloride rushes into the cell. This causes an
inhibitory hyperpolarization called an inhibitory postsynaptic potential (IPSP). After the stimulus, the
ion channels close, and the membrane potential returns to rest. The dotted, blue channels represent
sodium channels; the striped, green channels represent potassium channels; the solid yellow
channels represent chloride channels. ‘IPSP’ by Casey Henley is licensed under a Creative Commons
Animation 4
Figure 5.6. If the cell is at rest at chloride’s equilibrium potential, when a stimulus opens the chloride
channels, there will be no net movement of chloride in either direction because chloride will be at
equilibrium. Since there is no net movement, there will also be no change in membrane potential
because there is an equal amount of ion flow into and out of the cell. The dotted, blue channels
represent sodium channels; the striped, green channels represent potassium channels; the solid
yellow channels represent chloride channels. ‘IPSP at Equilibrium’ by Casey Henley is licensed under
License.
Animation 5
Figure 5.7. If the cell is at rest at chloride’s equilibrium potential, when a stimulus opens the chloride
channels, chloride will leave the cell, removing its negative charge. This causes a depolarization in
the membrane potential, but it is still inhibitory since chloride movement will try to keep the cell
near -65 mV. The dotted, blue channels represent sodium channels; the striped, green channels
represent potassium channels; the solid yellow channels represent chloride channels.‘Inhibitory
Depolarization’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 6
Figure 5.8. Excitatory stimuli that occur quickly in succession lead to summation of EPSPs. This leads
to increased depolarization of the membrane potential compared to a single EPSP. The dotted, blue
channels represent sodium channels; the striped, green channels represent potassium channels; the
solid yellow channels represent chloride channels. ‘Summated EPSPs’ by Casey Henley is licensed
Animation 7
Figure 5.9. When an inhibitory input and an excitatory input stimulate a postsynaptic neuron at the
same time, chloride and sodium channels open. Due to the equilibrium potentials of the two ions,
both will flow into the cell. Sodium tries to depolarize the cell, whereas chloride tries to keep the cell
near rest. The dotted, blue channels represent sodium channels; the striped, green channels
represent potassium channels; the solid yellow channels represent chloride channels. ‘EPSP and
IPSP Ion Flow’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Chapter 6
Animation 1
Animation 6.9. The action potential moves down the axon beginning at the axon hillock. The action
potential moving down a myelinated axon will jump from one Node of Ranvier to the next. This
saltatory conduction leads to faster propagation speeds than when no myelin in present. When the
action potential reaches the synaptic terminal, it causes the release of chemical neurotransmitter.
‘Action Potential Propagation’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 2
Figure 6.10. A. As EPSPs summate, a result of ion movement not shown in the figure, the cell’s
membrane potential will depolarize. B. Reaching threshold causes voltage-gated ion channels to
open. Once the channels are open, ions will move toward equilibrium. In the figure, sodium ions
flow inward. The dotted, blue channels represent voltage-gated sodium channels; the striped, green
channels. ‘Voltage-Gated Channel’ by Casey Henley is licensed under a Creative Commons
Animation 3
Figure 6.11. Voltage-gated sodium channels open once the cell’s membrane potential reaches
threshold. The rapid influx of sodium results in a large depolarization called the rising phase. The
dotted, blue channels represent voltage-gated sodium channels; the striped, green channels
represent voltage-gated potassium channels; the solid yellow channels represent chloride channels.
‘Rising Phase’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 4
Figure 6.12. After approximately 1 msec, the voltage-gated sodium channels inactivate, which
prevents any further ion flow into the cell. Although the voltage-gated potassium channels are
activated in response to the cell reaching threshold, their opening is delayed and occurs alone with
the sodium channel inactivation. This allows an efflux of potassium ions, which causes the
repolarization of the falling phase. The dotted, blue channels represent voltage-gated sodium
channels; the striped, green channels represent voltage-gated potassium channels; the solid yellow
channels represent chloride channels. ‘Falling Phase” by Casey Henley is licensed under a Creative
Animation 5
Figure 6.13. Once the cell’s membrane potential repolarizes, the voltage-gated sodium channels
de-inactivate and return to their closed state. The voltage-gated potassium channels remain open
long enough for the undershoot to occur as potassium continues to flow out of the cell. The dotted,
blue channels represent voltage-gated sodium channels; the striped, green channels represent
voltage-gated potassium channels; the solid yellow channels represent chloride channels.
‘Undershoot’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 6
Figure 6.14. Once the voltage-gated potassium channels close, the sodium-potassium pump will
work to re-establish the electrochemical gradients and return the cell to its resting membrane
potential. ‘Return to Rest’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 7
Figure 6.15. A) A voltage change that reaches threshold will cause voltage-gated sodium channels to
open in the axonal membrane. The influx of sodium causes the rising phase of the action potential,
but the ion flow also depolarizes nearby axon regions. B) As the depolarization reaches threshold,
the action potential moves down the axon. The dotted, blue channels represent voltage-gated
sodium channels; the striped, green channels represent voltage-gated potassium channels. ‘Action
Potential Movement’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 8
Figure 6.16. The action potential moves down an unmyelinated axon like a wave, opening
voltage-gated channels along the length of the axon. In a myelinated axon, though, the action
potential is able to skip portions of the axon that are covered by the myelin; the action potential
jumps from node to node and travels further down the axon in the same amount of time. The
dotted, blue channels represent voltage-gated sodium channels; the striped, green channels
represent voltage-gated potassium channels. ‘Action Potential Speed’ by Casey Henley is
Chapter 7
Animation 1
Figure 7.9. A) Clamping the cell at 0 mV will result in current being passed into the axon to
depolarize the membrane potential. This depolarization is above threshold, so the voltage-gated ion
channels in the membrane will be activated. B) Sodium will enter the axon through the open sodium
channels. The voltage clamp equipment will inject current equal in strength and opposite in charge
to the sodium influx in order to keep the membrane potential of the axon at 0 mV. The membrane
potential will remain at 0 mV because the injected current offsets any change that would normally
occur due to ion flow. ‘Voltage Clamp Sodium Flow’ by Casey Henley is licensed under a Creative
Animation 2
Figure 7.10. The voltage-gated sodium channels will inactivate, and the potassium channels will
open. Potassium will then flow out of the axon. Similar to the sodium influx, the voltage clamp
equipment will inject current equal in strength and opposite in charge to the potassium efflux in
order to keep the membrane potential of the axon at 0 mV. ‘Voltage Clamp Potassium Flow’ by
Chapter 8
Animation 1
Figure 8.4. Membrane-bound proteins called connexons form gap junctions between presynaptic
and postsynaptic neurons. This allows for direct exchange of ions between neurons. An action
potential in the presynaptic neuron will cause an immediate depolarization of the postsynaptic
membrane because the sodium ions will cross the membrane through the gap junctions. ‘Electrical
Synapse – Ion Flow’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 2
Figure 8.5. Since an electrical synapse is a direct, physical connection between two neurons, ions are
able to flow either direction across the gap junction. ‘Bidirectional Electrical Synapse’ by Casey
Animation 3
Figure 8.6. Gap junctions are large enough to allow the flow of small cellular molecules like ATP or
second messengers. ‘Electrical Synapse – Small Molecules’ by Casey Henley is licensed under a
License.
Animation 4
Figure 8.7 When an action potential arrives in the presynaptic terminal, neurotransmitters are
released into the synaptic cleft where they can act on neurotransmitter receptors in the
postsynaptic membrane. ‘Chemical Synapse – Neurotransmitter Release’ by Casey Henley is licensed
Chapter 10
Animation 1
Animation 10.5. The action potential is a brief but significant change in electrical potential across the
Animation 2
Figure 10.6. A) An action potential causes an influx of sodium in the terminal. B) The depolarization
opens voltage-gated calcium channels, and calcium ions flow into the terminal down their
electrochemical gradient. The blue, dotted channels represent voltage-gated sodium channels, and
the purple, striped channels represent voltage-gated calcium channels. ‘Terminal Calcium Influx’ by
Animation 3
Figure 10.7. A) Calcium enters the cell when the voltage-gated channels open. B) In the presence of
calcium, synaptotagmin, a protein bound to the vesicular membrane interacts with the SNARE
proteins. The purple, striped channels represent voltage-gated calcium channels. ‘Synaptotagmin’
Animation 4
Figure 10.8. Once the synaptotagmin-SNARE protein complex forms, the synaptic vesicle membrane
fuses with the terminal membrane, and the neurotransmitters are released into the synaptic cleft
through exocytosis. The purple, striped channels represent voltage-gated calcium channels.
‘Transmitter Exocytosis’ by Casey Henley is licensed under a Creative Commons Attribution
Chapter 11
Animation 1
Figure 11.2. A) Ionotropic receptors, also called ligand-gated channels, are ion channels that are
opened by the binding of neurotransmitters. Voltage-gated channels are opened by the membrane
potential of the cell reaching threshold. B) Both types of channels allow ions to diffuse down their
electrochemical gradient. The lined, teal channels represent glutamate receptors; the solid yellow
channels represent GABA receptors; the dotted, blue channels represent voltage-gated sodium
channels. ‘Ion Channel Gating’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 2
Figure 11.3. Since neurotransmitter receptors can only bind specific neurotransmitters. glutamate
binds to (A) and opens (B) glutamate receptors but has no effect on GABA receptors. The lined, teal
channels represent glutamate receptors; the solid yellow channels represent GABA receptors.
‘Ligand and Receptor’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 3
Figure 11.4. AMPA and kainate glutamate receptors are non-selective ion channels that allow both
sodium and potassium to flow across the membrane. When glutamate binds, sodium flows in and
potassium flows out. The lined, teal channel represents AMPA receptors; the checkered, teal channel
represents kainate receptors. ‘AMPA and Kainate’ by Casey Henley is licensed under a Creative
Animation 4
Figure 11.5. NMDA receptors are opened by a combination of glutamate binding and a voltage
trigger. A) At low levels of stimulation when the the membrane potential is near rest and below the
NMDA receptor voltage threshold, a magnesium ion blocks the open NMDA receptor channel
preventing ion flow. Ions can flow through open AMPA receptors, which begins to depolarize the
membrane. B) Once the NMDA receptor voltage threshold has been reached, the magnesium ion is
expelled from the channel, allowing sodium, potassium, and calcium to cross the membrane. The
lined, teal channels represent AMPA receptors; the dotted, violet channels represent NMDA
receptors. ‘AMPA and NMDA’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 5
Figure 11.6. GABA and glycine are inhibitory receptors that are selective to chloride. The solid yellow
channel represents a GABA receptor; the patterned, yellow channel represents a glycine receptor.
‘GABA and Glycine’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 6
Figure 11.7. Ions move through open voltage-gated channels trying to reach equilibrium. As the ions
cross the membrane, the neuron’s membrane potential moves closer to the ion’s equilibrium
potential. In the figure, a voltage-gated sodium channel opens, and sodium flows in until the
membrane potential equals approximately +60 mV, sodium’s equilibrium potential. The blue, dotted
channel represents a voltage-gated sodium channel. ‘Equilibrium Potential’ by Casey Henley is
Animation 7
Figure 11.8. Ions move through open ligand-gated channels trying to reach equilibrium. As the ions
cross the membrane, the neuron’s membrane potential moves closer to the receptor’s reversal
potential. When the ionotropic receptor only increases permeability for one ion, the receptor’s
reversal potential is the same as the ion’s equilibrium potential. In the animation, a GABA receptor
open, and chloride flows in until the membrane potential equals approximately -65 mV, GABA’s
reversal potential and chloride’s equilibrium potential. The yellow, checkered channel represents a
GABA receptor. ‘GABA Reversal Potential’ by Casey Henley is licensed under a Creative Commons
Animation 8
Figure 11.9. The reversal potential of an ionotropic receptor that is not selective to one ion will fall
between the equilibrium potentials of the permeable ions. Glutamate receptors allow the flow of
both sodium and potassium ions, so the reversal potential for the receptor is approximately 0 mV.
More sodium will flow into the cell than potassium flows out, resulting in a depolarization of the
membrane. The line, teal channel represents a glutamate receptor. ‘Glutamate Reversal Potential –
Rest’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 9
Figure 11.10. At the reversal potential, there is no net ion flow in either direction. An equal number of
sodium ions enter the cell as potassium ions leave. Since there is no change in voltage at the
reversal potential, if the receptor remained open, the membrane potential would stay at 0 mV.
‘Glutamate Reversal Potential – 0 mV’ by Casey Henley is licensed under a Creative Commons
Chapter 12
Animation 1
Figure 12.5. A) Neurotransmitter binding to a G-protein-coupled receptor causes the inactivated

G-protein complex to interact with the receptor. B) The GDP molecule is then exchanged for a GTP
molecule, which activates the G-protein complex. ‘G-protein Binding’ by Casey Henley is licensed
Animation 2
Figure 12.6. A) Once activated, the G-protein complex will separate into the alpha-GTP subunit and
the beta-gamma subunit. B) These subunits can stimulate or inhibit effector proteins within the cell.
‘G-protein Effects’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 3
Figure 12.7. Some GPCRs, like the muscarinic acetylcholine receptors in the heart, alter cellular
permeability by opening ion channels. The activated beta-gamma subunit of the muscarinic receptor
(A) opens GIRK potassium channels and allows the efflux of potassium (B). ‘Beta-Gamma Ion
Channels’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 4
Figure 12.8. GPCRs that couple to the Gs alpha subunit initiate the adenylyl cyclase / cAMP pathway.
The Gs subunit activates adenylyl cyclase, which then converts ATP to cAMP. cAMP binds to and
activates protein kinase A (PKA), which phosphorylates proteins in the cell. ‘Adenylyl Cyclase
Pathway’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Animation 5
Figure 12.9. A) The adenylyl cyclase / cAMP pathway can alter many cellular functions. One example
is that both cAMP and PKA can open ion channels. B) Like ligand-gated channels, there are also
cAMP-gated channels, which open after cAMP binding. PKA is able to phosphorylate and modulate
ion channel function by converting ATP to ADP. ‘Second Messenger Ion Channel Action’ by Casey
Animation 6
Figure 12.10. A) PKA can phosphorylate a number of proteins involved with neuron function. B) It
can target proteins involved with neurotransmitter synthesis, packing, and release, or it can enter
the nucleus and phosphorylate CREB, a transcription factor that can initiate gene transcription and
protein synthesis. ‘PKA Targets’ by Casey Henley is licensed under a Creative Commons Attribution
Animation 7
Figure 12.11. A) The Gq G-protein subunit activates phospholipase C, which converts the
phospholipid PIP2 in the cell membrane into DAG, another membrane-bound molecule, and IP3, a
cytoplasmic molecule. B) DAG can interact with PKA, initiating phosphorylation of cellular proteins.
IP3 opens calcium channels in the endoplasmic reticulum, allowing calcium to flow into the
cytoplasm. C) Calcium, another second messenger can have many cellular effects. It can bind to
calmodulin, which then activates CaMK, causing phosphorylation of more protein targets. ‘IP3-DAG
Pathway’ by Casey Henley is licensed under a Creative Commons Attribution Non-Commercial
Chapter 19
Animation 1
Figure 19.16. A) In the dark, the photoreceptor is depolarized due to an influx of sodium and calcium
through open ion channels that are gated by cGMP. The photoreceptor has high levels of cGMP
when it is in the dark. Additionally, the opsin proteins, the G-protein transducin, and
phosphodiesterase (PDE) are all inactivated. B) Light reaching the photoreceptor causes a
conformational change in the opsin protein, which activates the G-protein transducing. Transducin
activates phosphodiesterase (PDE), which converts cGMP to GMP. Without cGMP, the cation
channels close, stopping the influx of positive ions. This results in a hyperpolarization of the cell.
‘Phototransduction’ by Casey Henley is licensed under a Creative Commons Attribution

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Introduction to Neuroscience

Michigan State University Libraries

Part I. Introduction to Neuroscience

Part II. Cells of the Nervous System Structure & Function

2. Cells of the Nervous System: The Neuron 25

11. Synapse Structure 131

Part IV. Nervous System Organization

21. Anatomical Terminology 221

28. General Principles of Sensory Systems 335

Part VI. Motor Systems

40. Spinal Motor Control and Proprioception 563

47. Neurodegenerative Disease- Non Motor 683

Additional Review Answers 949

Introduction to Neuroscience is designed for undergraduate students enrolled in a typical 2-semester

About the Author

Figure 1.1. Brain

Neuroscience is an integrative field of study

conceptualize. It would be nearly impossible to understand that many components of a system

Figure 1.2 Areas

How do we learn about neuroscience?

Figure 1.3 The

Figure 1.4 Image

The Use of Animals in Research

1. Animals should only be used in worthwhile experiments.

Performing an experiment in an intact, living organism, whether human or nonhuman, is

Falling in between these two preparations is an ex vivo experiment. In this kind of

What neuroscience is not

Myth 1: “We only use 10% of our brain.”

Myth 2: “Forming memories causes new neurons to be

Myth 3: “The brain cannot repair itself.”

Myth 4: “If you are analytical, you are left brain

dominant, but if you are creative, you are right brain

Figure 1.8. Image of Paul Broca.

CELLS OF THE NERVOUS SYSTEM: THE

There are 2 major cell types within the nervous system:

Figure 2.9. Since

Presynaptic versus Postsynaptic

• Each structural component of the neuron has an important function

CELLS OF THE NERVOUS SYSTEM: GLIA

Although most of neuroscience is concerned with

Figure 3.2 Image

Figure 3.5 Image

VISUALIZING CELLS OF THE NERVOUS

For the majority of human history, the only way we were

Visualizing Cells: Cell Staining

Nissl-Staining (Cresyl Violet Staining)

Figure 4.1 Nissl

Santiago Ramon y Cajal

• Nissl stains stain neuronal cell bodies

Ion flow into and out of the neuron is a critical

Ion Channels Allow Ion Movement

Attribution Non-Commercial (CC-BY-NC) 4.0 International License. View static image of

Gradients Drive Ion Movement

static image of animation.

When Gradients Balance, Equilibrium Occurs

Important Ions for Neurons

Ion Inside concentration (mM) Outside concentration (mM)

Sodium (Na+) 15 145

Potassium (K+) 125 5

Chloride (Cl-) 13 150