MGM UNIVERSITY, AURANGABAD

INSTITUTE OF BIOSCIENCES AND TECHNOLOGY

CHOICE-BASED CREDIT SYSTEM (CBCS)

SEMESTER PATTERN

Faculty of Engineering and Technology Graduate (UG)

program

Biotechnology - CURRICULUM
w.e.f. Academic Year 2023-24

B.Tech, B. Tech. (Hons.), B. Tech. (Hons.) with Research of

Biotechnology

SEMESTER (I and II)

 MGM UNIVERSITY, AURANGABAD
INSTITUTE OF BIOSCIENCES AND TECHNOLOGY

CHOICE BASED CREDIT SYSTEM (CBCS)

SEMESTER PATTERN

Faculty of Engineering and Technology Graduate (UG) program

BIOTECHNOLOGY - CURRICULUM

B.Tech. Biotechnology

FIRST YEAR

SEMESTER-I

CURRICULUM
 MGM University
Chhatrapati Sambhajinagar– 431003
(Template format as per discussion at 14/05/2023)
Name of the College/Institute: Institute of Bioscience and Technology Faculty of Engineering and Technology
Name of the Program :( 3/4 Years UG programme) B.Tech. Biotechnology
Program Type: UG B.Tech Biotechnology Duration: - 04 Years (08 Semesters)

First Year (Semester I)

Course Course code Course Title Type Teaching period Credits Durat Evaluation Scheme (Marks) Minimum Passing (Marks)
Type per week ion of
Internal External Total Internal External Tota
(Hrs /week) exam
l
L T P CA-I MSE CA- TW ESE PR CA/MSE/TW ESE PR
II
BSC BBT42MML101 Basic Cell Biology Theory 3 3 20 20 20 - 40 - 100 16 40
BSC BBT42MML102 General Biochemistry Theory 3 - 3 20 20 20 - 40 - 100 16 40
ESC BBT42MML103 Biology Concept, Connection Theory 2 - 2 10 10 10 - 20 - 50 08 - 20
and Innovation
ESC BBT42MML104 Microbial Physiology Theory 2 - 2 10 10 10 - 20 - 50 08 - 20
AEC Ability Enhancement Course Theory 2 - - 2 10 10 10 - 20 - 50 08 20
VSEC BBT42VSP101 Mini project Practical - 4 2 30 20 50 08 20
BSC BBT42MMP101 Biochemistry Lab Practical - 4 2 30 20 50 08 20
ESC BBT42MMP102 Cell Bio Lab Practical - 4 2 30 20 50 08 20
ESC BBT42MMP103 Microbiology Lab Practical - - 2 2 - 30 - 20 50 08 20
CC Co-Curricular Activity Practical - 4 2 - 30 - 20 50 08 20
Total (L-T-P) Hrs / week = 30 12 18 22 550 220
 First Year (Semester II)
Teaching Evaluation Scheme Minimum Passing
period per
Duration Internal External Internal External
Course Course code Course Title Type week Credit
of exam Total Total
CA-
L T P CA-I MSE TW ESE PR CA/MSE/TW ESE PR
II
BSC BBT42MML105 Enzymology Theory 3 3 10 10 10 - 20 - 50 08 20
BSC BBT42MML106 Molecular Biology of Gene Theory 3 - 3 10 10 10 - 20 - 50 08 20
ESC BBT42MML107 Immunology Theory 2 - 2 20 20 20 - 40 - 100 16 40
ESC BBT42MML108 Numerical and Computational Theory 2 - 2 20 20 20 - 40 - 100 16 40
Fundamentals-I
PCC Mendel And Darwin Theory Theory 2 - 2 20 20 20 - 40 - 100 16 40
VSEC* BFT4VSP105 Mini project Practical - 4 2 30 20 - 50 08 20
ESC BBT42MMP104 Immuno Lab Practical 4 2 30
ESC BBT42MMP105 Computational Lab Practical - 4 2 30 20 - 50 08 20
BSC BBT42MMP106 Enzymo Lab Practical - - 4 2 - 30 - 20 50 08 20
CC Co-Curricular Activity Practical - - 4 2 - 30 - 20 50 08 20
Total (L-T-P) Hrs / week = 32 12 20 22 600 200

*As per the requirement VSC / SEC can be used for Theory or Practical of core subject **as per the requirement, Department/Institute can offer OE practical
Award of UG certificate with 44 credits and an additional 4-credits core NSQF course / Internship OR continue with major and minor
 SYLLABUS OF SEMESTER FIRST WITH LAYOUT

First Year (Semester I)

Course Course Course Title Type Teaching period Credits Durat Evaluation Scheme (Marks) Minimum Passing (Marks)
Type code per week ion of
Internal External Total Internal External Tota
(Hrs /week) exam
l
L T P CA-I MSE CA- TW ESE PR CA/MSE/TW ESE PR
II
BSC BBT42MM Basic Cell Biology Theory 3 3 20 20 20 - 40 - 100 16 40
L101
BSC BBT42MM General Biochemistry Theory 3 - 3 20 20 20 - 40 - 100 16 40
L102
ESC BBT42MM Biology Concept, Connection Theory 2 - 2 10 10 10 - 20 - 50 08 - 20
L103 and Innovation
ESC BBT42MM Microbial Physiology Theory 2 - 2 10 10 10 - 20 - 50 08 - 20
L104
AEC Ability Enhancement Course Theory 2 - - 2 10 10 10 - 20 - 50 08 20
VSEC BBT42VSP Mini project Practical - 4 2 30 20 50 08 20
101
BSC BBT42MM Biochemistry Lab Practical - 4 2 30 20 50 08 20
P101
ESC BBT42MM Cell Bio Lab Practical - 4 2 30 20 50 08 20
P102
ESC BBT42MM Microbiology Lab Practical - - 2 2 - 30 - 20 50 08 20
P103
CC Co-Curricular Activity Practical - 4 2 - 30 - 20 50 08 20
Total (L-T-P) Hrs / week = 30 12 18 22 550 220


BASIC CELL BIOLOGY

University: MGM University, Aurangabad Faculty: Engineering and Technology Institute:

Institute of Biosciences and Technology. Degree: B.Tech. Biotechnology Course Unit
Code: Course Title: Basic cell biology
Credits allocated: 3+0 Level of Study: UG
Recommended Year /Semester: B.Tech. Biotechnology Year 1/ Semester I
Prerequisites for registration: Registration of a student in various courses in consultation with
the respective course teacher and Adviser and acceptance by the principal. The approved
courses must be mentioned in the roster form.

Course Objective:
1. Students will understand the structures and purposes of basic components of
prokaryotic and eukaryotic cells, especially macromolecules, membranes, and organelles
2. Students will understand how these cellular components are used to generate and
utilize energy in cells
3. Students will understand the cellular components underlying mitotic cell division
4. Students will apply their knowledge of cell biology to selected examples of changes or
losses in cell function. These can include responses to environmental or physiological
changes, or alterations of cell function brought about by mutation.

Course Outcomes:

COURSE CONTENT
THEORY
Unit I: Cells and Genome (8 Lecture )
Introduction to cell: Cells and Genome: The Universal features of cells on Earth, Genetic
Information in Eukaryotesm .The chemical component of a cell Proteins: The Shape and
Structure of Proteins. Protein Function.
Unit II: Internal Organization of the cell (8 Lecture )
Membrane Structure: The Lipid Bilayer, Membrane proteins, Membrane transport of small
molecules and the electrical properties of membranes: Principles of membrane transport,
Transporters and active membrane transport, Channels and the electrical properties of
membranes.
Unit III: Ways of Working with Cells (8 Lecture )
Analysing cells, molecules, and systems: Isolation cells and Growing them in culture,
Purifying Proteins, Analyzing Proteins, Analyzing and Manipulating DNA. Electron
Microscope, Compound Microscope.
Unit IV: Cell Signaling (8 Lecture )
The Principals of Cell Signaling, signaling through G- protein- Coupled Receptors. The Cell
Cycle, Cell Death, Cell Junction and the Extracellular Matrix
Unit V: Pathogens and Infection (8 Lectures )
The Innate and Adaptive Immune Systems. Antigens, Antibody, Antigen-Antibody
interaction, ELISA.
Reference Book/ suggested Reading/ Textbook
1. Molecular Biology of the Cell (6th Edition) ...
2. The Cell: A Molecular Approach (7th Edition) ...
3. Essential Cell Biology (4th Edition) ...
4. Mitochondria and the Future of Medicine (1st Edition) ...
5. The Double Helix (1st Edition) ...
6. The Epigenetics Revolution (1st Edition)
 General Biochemistry

University: MGM University, Aurangabad Faculty: Engineering and Technology

Institute: Institute of Biosciences and Technology. Degree: B.Tech. Biotechnology
Course Code: Course Title: General Biochemistry
Credits allocated: 3+0 Level of Study: UG

Recommended Year /Semester: B.Tech. Biotechnology Year 1/ Semester I

Prerequisites for registration: Registration of a student in various courses in consultation

with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form

Course Objective:
1.The student will have an understanding of the metabolic processes by which energy is
produced in cells and amino acids, lipids, purines and pyrimidines, and carbohydrates are
synthesized.
2.The student will be able to describe the roles of vitamins in metabolic processes and
enzyme activity.
Course Outcomes:
1. The learning outcomes are designed to help learners understand the objectives of
studying Biochemistry that is, to analyse, appreciate, understand the basic concepts of
chemical reactions that occur in living systems
2. The student will be able to identify the structural elements of proteins, the basic
features of enzyme catalysis and regulation, and the function of hemoglobin in oxygen
binding and transport.
3. The student will be able to describe the basic structural features of nucleic acids
COURSE CONTENTS
THEORY
Unit I: Introduction: Biochemistry & it’s scope (8 Lectures )
Cellular Biochemistry - Cell-structure – plant and animal, composition and function of cell
organelle Carbohydrates: Occurrence, Classification & Structures; Physicochemical and
Metabolic functions.
Unit II: Biological role of carbohydrates (8 Lectures )
Metabolism of carbohydrates - glycolysis and respiration, production of ATP, brief
description of electron transport chain, oxidative and substrate phosphorylation Proteins:
Occurrence, Classification & Structures; Physicochemical & Metabolic functions.
Unit III: Metabolism of proteins (8 Lectures )
Breakdown of proteins, transamination, deamination, decarboxylation, nitrogen fixation, urea
cycle; Lipids: Occurrence, Classification & Structure; Physicochemical and metabolic
functions.
Unit IV: Biological Role of Lipid (8 Lectures )
classification and biosynthesis; Biological role of lipids; breakdown of triglycerides and
phospholipids; β‐oxidation of long chain fatty acids, ketosis, biosynthesis of fatty acids,
triglycerides and phospholipids.
Unit V: Nucleic Acids (8 Lectures )
Classification, structure & biosynthesis of nucleic acid; Metabolism RNA and DNA
metabolism. Vitamins; Sources and classification, Chemistry and Metabolic functions,
deficiency syndromes, Minerals; Sources and classification, Chemistry and Metabolic
functions, deficiency syndromes.

TEXT BOOK
1.Fundamentals of Biochemistry Jain JL, Jain S and Jain N S. Chand Publication, India 2016
2.Biochemistry Satyanarayana Elsevier, 2013
3.Lehninger Principles of Biochemistry David L. Nelson and Michael M. Cox 6th Ed.
Macmillan Learning, NY, USA. 2012
4.Outlines of Biochemistry Conn EE and Stumpf PK 4 th Edition Wiley Eastern Ltd, Pune
(India)
Reference Book/ suggested Reading/ Textbook:
1.Wardlaw's Perspectives in Nutrition: A Functional Approach Gaile Moe, Danita Kelley,
Byrd-Bredbenner Jacqueline Berning and Carol Mc Graw-Hill, Inc., NY, USA. 2013
2.Biochemistry Carolyn D. Berdanier, Elaine B. Feldman and Johanna Dwyer 4th Ed. John
Wiley and Sons, Inc.,NY, USA. 2011
3.Handbook of Nutrition and Food Donald Voet and Judith G. Voet 2nd Ed. CRC Press, Boca
Raton,FL, USA. 2008
4.Biochemistry & Molecular Biology of Plants Bob B. Buchanan, Wilhelm Gruissem and
Russell L. Jones John Wiley and Sons, Inc., NY, USA. 2002
 Biology Concept, Connection, Innovation and Application

University: MGM University, Aurangabad Faculty: Engineering and Technology

Institute: Institute of Biosciences and Technology. Degree: B.Tech. Biotechnology Course
Code: Course Title: Biology Concept,
Connection, Innovation and Application
Credits allocated: 2+0 Level of Study: UG
Recommended Year /Semester: B.Tech. Biotechnology Year 1/ Semester I
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the principal. The approved
courses must be mentioned in the roster form
Course Objective:
1.To provide an introduction to basic biological concepts including the metric system,
physical and chemical properties of life, cell structure and function, cell reproduction, and
metabolism.
2.To unable student to do Critical thinking skills, study skills, and basic math skills are also
included.
Course Outcomes:
Specific skills and competencies expected of students who complete this course include:
1.Describe basic physicochemical concepts that underlie the structure and function of cells
2.Describe the structure and function of the four classes of biomolecules
3.Describe the structure and function of eukaryotic cells and their organelles
4.Describe the processes by which substances move into and out of cells
5.Describe the structure and function of enzymes
6.Discuss energy transfer in cells and the pathways of cellular respiration
7.Describe DNA replication, protein synthesis, mitosis and meiosis
8.Analyse, synthesize, evaluate and apply information
9.Apply mathematical, logical and scientific principles and methods
10.Demonstrating higher level critical thinking skills, solving problems, and following
directions
11.Directing their own learning activities to meet course objectives
COURSE CONTENTS
THEORY
Unit I: The molecular basis of Life/ Cell (6Lectures )
The science of biology, the scientific study of life, the nature of molecules and the properties
of water, the chemical Building Block of life / chemistry of life, Cell structure and function,
Membrane structure and function, Metabolism energy and enzymes, how cells harvest
energy, Photosynthesis, Cell communication, how cell divide / cell division, Plant
organization and function, Plant reproduction and responses, how cells release energy
Unit II: Genetics and Molecular biology (6 Lectures )
Sexual Reproduction and Meiosis, Patterns of inheritance, Human inheritance, Chromosome,
mapping and the Meiosis-inheritance connection, DNA-The genetic material, Genes and how
they work, DNA Replication, Binary fission and Mitosis, Control of gene expression, DNA
Technology, Genomics, Cellular Mechanisms of development
Unit III: Cells and Energy (6 Lectures )
Cells: The working Units of Life, Features of Prokaryotic and Eukaryotic cell, chemical
structure and functions of lipids, Cell Membranes, Cell communication and multicellularity,
Signal transduction pathway, Signal Receptors initiate a cellular response, Energy: types of
energy, types of metabolism, Laws of thermodynamics, Role of ATP in biochemical
energetics, Enzymes: Introduction, Working of enzymes, Regulation of enzymes
Unit IV: Genes and Heredity (6 Lectures )
The cell cycle and cell division, Inheritance, Genes and chromosomes, DNA and Its Role in
Heredity, From DNA to Protein: Gene Expression, Gene Mutation and Molecular Medicine,
Regulation of Gene Expression.
Unit V: Genomes ( 6 Lectures )
Genomes, Recombinant DNA and B.Tech. Biotechnology, Differential gene Expression in
Development, Genes its development and Evolution

Reference Book/ suggested Reading/ Textbook:

1.Biology -Concepts & Applications by Cecie Starr Christine A. Evers Lisa Starr
2.BIOLOGY- Concept and investigation by Mariëlle Hoefnagels
3.Campbell Biology: Concepts & Connections by Martha R. Taylor, Eric J. Simon, Jean L.
Dickey, Kelly A. Hogan, Jane B. Reece
4.Life: The Science of Biology by David E. Sadava , David M. Hillis, et al.
5.Biology by Peter Raven, George Johnson, Kenneth Mason, Jonathan Losos , Susan Singer
 Microbial Physiology

University: MGM University, Aurangabad Faculty: Engineering and Technology Institute:

Institute of Biosciences and Technology. Degree: B.Tech. Biotechnology
Course Code: Course Title: Microbial Physiology
Credits allocated: 2+0 Level of Study: UG
Recommended Year /Semester: B.Tech. Biotechnology Year 1/ Semester I
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the principal. The approved
courses must be mentioned in the roster form
Learning Outcomes:
After completion of the lecture component of the course, successful students will:
1.Demonstrate an understanding of cellular superstructure and the functional components of
cells.
2.Demonstrate an understanding of how organisms build and maintain a proton motive force.
3.Comprehend the how cells metabolize the nutrients necessary for life including carbon,
nitrogen, sulphur and phosphorus.
4.Appreciate how biochemical pathways and processes are integrated into a network, which
provides robustness to life.
5.Comprehend how cellular physiology is altered by interactions between microbes and the
environment. 6. Appreciate that the diversity of life is driven by the metabolic diversity of
microbes.
Objective:
Students are expected to gain a fundamental understanding of the organization and
metabolism of microbial cells to further comprehend the robust and diverse nature of life.
COURSE CONTENTS
THEORY
Unit I: Microbial Growth (6 Lectures )
Definition of growth, balanced and unbalanced growth, growth curve, the mathematics of
growth-generation time, specific growth rate, batch and continuous culture, Synchronous
growth, diauxic growth curve. pH-classification based on pH ranges and adaptations
Unit II: Microbial Transport and Metabolism (6 Lectures )
Diffusion – Passive and facilitated, Primary active and secondary active transport, Group
translocation (phosphotransferase system), symport, antiport and uniport, electrogenic and
electro neutral transport. Chemolithotrophic metabolism- Carbondioxide fixation: Calvin
cycle and reductive TCA cycle. physiology of bacterial photosynthesis: light reactions, cyclic
and non- cyclic photophosphorylation.
Unit III: Enzymes and Regulation (6 Lectures )
Enzymes: Importance, structure and classification of enzymes. Apoenzyme and cofactors.
Prosthetic group, coenzyme and metal cofactors. Active site and its salient features.
Mechanism of enzyme action. Activation energy, Lock and key hypothesis, induced fit.
Enzyme kinetics and inhibition, Effect of pH and temperature on enzyme activity. rreversible
and reversible inhibition: competitive and non-competitive inhibition. Activity: allostery,
covalent modification and feedback inhibition.
Unit IV: Microbial energetics and Nitrogen Fixation (6 Lectures )
Concept of aerobic respiration, anaerobic respiration and fermentation. Central metabolic
pathways: EMP pathway, ED pathway, PP pathway, and TCA cycle. Gluconeogenesis.
Nitrogen Fixation – Physiology of nitrogen cycle. Assimilatory and dissimilatory nitrate
reduction, biological nitrogen fixation. Nitrogen fixers and mechanism of nitrogen fixation.
Unit V: Microbial Respiration (6 Lectures )
Components of respiratory chain, and their inhibitors. Anaerobic respiration, denitrification,
nitrate/nitrite respiration. Oxidative phosphorylation: ATP synthesis and ATP synthase.
Uncouplers, Chemical coupling, conformational coupling
Reference Book/ suggested Reading/ Textbook:
1.Atlas RM. (1989). Microbiology: Fundamentals and Applications. 2nd Edition,MacMillan
Publishing Company, New York.
2.Conn EE and Stumpf PK. (1976). Outlines of Biochemistry. John Wiley & Sons.
3.Gallon JR and Chaplin AE. (1987). An Introduction to Nitrogen Fixation. CassellEducation
Ltd.
4.Gottschalk G. (1986). Bacterial Metabolism. 2nd edition. Springer Verlag.
5.Lehninger A. (1982). Biochemistry. Worth Publ.
6.Moat AG and Foster JW. (2002). Microbial Physiology. John Wiley and Sons
 MINI PROJECT

University: MGM University, Aurangabad Faculty: Engineering and Technology Institute:

Institute of Biosciences and Tech. Degree: B.Tech. Biotechnology
Course Code: Course Title: Micro Project
Credits allocated: 0+2 (1 Practical) Level of Study: UG

Mode of delivery, planned learning activities and teaching method: Project 2 hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester I

Project Assessment:
Ideas of project:
Defining projects ideas is crucial for setting realistic expectations and laying out a clear
vision for a project life cycle. Project-based learning not only provides opportunities for
students to collaborate or drive their own learning, but it also teaches them skills such as
problem solving, and helps to develop additional skills integral to their future, such as critical
thinking and time management.
Literature survey:
A literature review establishes familiarity with and understanding of current research in a
particular field before carrying out a new investigation. Conducting a literature review should
enable you to find out what research has already been done and identify what is unknown
within your topic.
Attendance:
In ongoing semester attendance are important for students. They are expected to do their
project in the semester that is timetabled. The criteria of attendance are given below.
 BIOCHEMISTRY LAB
University: MGM University, Aurangabad Faculty: Engineering and Technology Institute:
Institute of Biosciences and Tech. Degree: B.Tech. Biotechnology
Course Code: BBL-117 Course Title: Biochemistry Lab
Credits allocated: 0+2 (Practical) Level of Study: UG
Mode of delivery, planned learning activities and teaching method: Practical 2 hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester I
Detail syllabus
1.Qualitative analysis of carbohydrates (Glucose, Fructose and starch)
2.Identification tests for Proteins (albumin and Casein)
3.Quantitative analysis of reducing sugars (DNSA method) and Proteins (Biuret method)
4.Preparation of buffer solution and measurement of pH.
5.Study of enzymatic hydrolysis of Starch.
6.Determination of Salivary Amylase activity.
7.Amino acids and protein qualitative tests
8.Estimation of proteins by Lowry’s method
9.Lipids Qualitative test
10.Separation of amino acids by TLC
11.To study the Fehling and Benedict test for carbohydrates
12.To study Sumner’s Method for reducing sugar
13.To study Anthron method
14.Quantification of RNA by Orcinol method
15.To estimate amount of protein by Biuret method.
 Cell Biology Lab
University: MGM University, Aurangabad Faculty: Engineering and Technology
Institute: Institute of Biosciences and Tech. Degree: B.Tech. Biotechnology
Course Code: Course Title: Cell biology Lab
Credits allocated: 0+1 (1Practical) Level of Study: UG
Mode of delivery, planned learning activities and teaching method: Practical 2 hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester I

COURSE OBJECTIVES:
1. To demonstrate various techniques to learn the morphology, identification and
propagation of cells and microbes.
2. To learn the staining techniques and culturing of microorganism.

COURSE OUTCOMES:
Students will be able to
1. Understand the advanced technical information pertaining to laboratory bio-safety and
preventive measures from pathogenic microorganism.
2. Identify the various stages of mitosis

Detail syllabus
1. Identification of different stages of meiosis in onion buds
2. Identification of different stages of mitosis in onion tips
3. Visualisation of nuclear fraction by Acetocarmine stain
4. Staining and visualisation of mitochondria by Janus green stain
5. ABO blood grouping
6. RH factor determination
7. Widal test, Syphilis Fast Latex Agglutination Test
8. Immune precipitation test: single radial immunodiffusion, Double immune diffusion
9. Immune electrophoresis.
10. Make a differential count and subset population in lymphocyte (B & T lymphocytes)
11. Cell permeability testing- osmotic fragility
12. Isolation of cell organelle by differential centrifugation techniques from plant / animal
sources Isolation of mitochondrial DNA
13. Isolation of chloroplast DNA
14. Cell motility studies ( bacteria, algae, cyanobacteria, protozoans,)
15. Cell death /apoptosis studies using flow-cytometry demonstration
16. Isolation and identification of mutagens of plant origin (demonstration/ video)
17. Isolation of mitochondria from cauliflower
18. Study lipid solubility of memeberane
19. Effect of detergent on RBC
20. Study osmosis in blood sample

References :
1. Biotechnology: A laboratory Skills Course, J. Kirk Brown 2011
2. Methods in Biotechnology, SB Hong, MB Rashid, LZ Santiago-Vazquez 2017
 Microbiology Lab

University: MGM University, Aurangabad Faculty: Engineering and Technology

Institute: Institute of Biosciences and Tech. Degree: B.Tech. Biotechnology Course
Code: Course Title: Microbiology Lab
Credits allocated: 0+1 (1 Practical) Level of Study: UG
Mode of delivery, planned learning activities and teaching method: Practical 2 hrs / weekly

Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester I

Detail syllabus
2. To study laboratory rules
3. To study compound microscope
4. To study autoclave
5. To Study Hot air oven
6. To study lab equipment
7. To study centrifuge
8. To study laminar air flow
9. To study water bath
10. To study incubator shaker
11. To study spectrophotometer
12. To prepare bacterial culture media
13. To study streak plate and
14. To spread plate method
15. To study pour plate method
16. Simple staining
17. Negative staining
18. Gram staining.
19. Lactophenol Cotton Blue staining for fungi.
20. To study the preparation of Potato Dextrose Agar for cultivation of fungi.
21. To study storage of microorganinsm.
22. To study the growth curve of E.coli
23. To perform IMViC test

References:
1) Atlas, R. M. (1997) Principles of Microbiology, 2nd edition, W.M.T.Brown Publishers,
Dubuque, USA.
2) Cappuccino J and Sherman N. (2010) Microbiology: A Laboratory Manual, 9th edition,
Pearson Education Limited, New Delhi
3) Parija S.C. (2005) Text Book of Practical Microbiology, 1st edition, Ahuja Publishing
House, New Delhi.
4) Dubey RC and Maheshwari DK (2004) Practical Microbiology, 1st edition, S. Chand and
Co., Delhi.
5) Harley, J. P. and Prescott L. M. (2002) Laboratory Exercises in Microbiology, 5th edition,
The McGraw-Hill Co., New York
6) Benson H. (2001) Microbiological Applications Lab Manual, 8 th edition, The McGrawHill
Companies, New York
7) Aneja K.R. (1996) Experiments in Microbiology, 3rd edition, WishwaPrakashan, New
Delhi.
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MGM UNIVERSITY, AURANGABAD

INSTITUTE OF BIOSCIENCES AND TECHNOLOGY

CHOICE BASED CREDIT SYSTEM (CBCS)

SEMESTER PATTERN

Faculty of Engineering and Technology

Graduate (UG) program

BIOTECHNOLOGY - CURRICULUM

B.Tech Biotechnology

FIRST YEAR

SEMESTER-II

CURRICULUM
 SYLLABUS OF SEMESTER SECOND WITH LAYOUT

First Year (Semester II)

Teaching Evaluation Scheme Minimum Passing
period per
Duration Internal External Internal External
Course Course code Course Title Type week Credit
of exam Total Total
CA-
L T P CA-I MSE TW ESE PR CA/MSE/TW ESE PR
II
BSC BBT42MML105 Enzymology Theory 3 3 10 10 10 - 20 - 50 08 20
BSC BBT42MML106 Molecular Biology of Gene Theory 3 - 3 10 10 10 - 20 - 50 08 20
ESC BBT42MML107 Immunology Theory 2 - 2 20 20 20 - 40 - 100 16 40
ESC BBT42MML108 Numerical and Computational Theory 2 - 2 20 20 20 - 40 - 100 16 40
Fundamentals-I
PCC Mendel And Darwin Theory Theory 2 - 2 20 20 20 - 40 - 100 16 40
VSEC* BFT4VSP105 Mini project Practical - 4 2 30 - 20 50 08 20
ESC BBT42MMP104 Immuno Lab Practical 4 2 30 - 20
ESC BBT42MMP105 Computational Lab Practical - 4 2 30 - 20 50 08 20
BSC BBT42MMP106 Enzymo Lab Practical - - 2 2 - 30 - 20 50 08 20
CC Co-Curricular Activity Practical - - 4 2 - 30 - 20 50 08 20
Total (L-T-P) Hrs / week = 30 12 18 22 600 200
 ENZYMOLOGY

University- MGM University, Aurangabad Faculty- Engineering and Technology Institute-

Institute of Biosciences and Technology Degree- B.Tech. Biotechnology
Course Code- BTB 122 Course Title- Enzymology
Credits- 3+0 (4 Theory) Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 3hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester II
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses
must be mentioned in the roster form
Objective:
Primary goals of this course is to provide the students with detailed knowledge in
enzyme activity and kinetics, their mechanism of action and regulation and about the way of
enzyme application and exploitation.
Outcomes:
The course include deepening knowledge in the areas of purification and isolation of
enzymes, classification of enzymes and cofactors, kinetics and regulation of enzymes and
their applications in industry, therapeutics and diagnosis.

THEORY
UNIT I: CLASSIFICATION AND ENZYME ACTION (6 lectures )
Nomenclature and classification of enzymes. Definition of holo-enzyme, apo-enzyme,
coenzyme, cofactor. Introduction to Biocatalysis, differences between chemical and
biological catalysis. Active site, Enzyme specificity. Principles of energy of activation,
transition state. Interaction between enzyme and substrate-lock and key, induced fit models.
Fundamentals of
enzyme assay, enzyme units.
UNIT II: ENZYME KINETICS AND INHIBITION (6 lectures )
Kinetics of single substrate enzyme - catalysed reactions - Michaelis - Menten equation,
importance of Vmax, Km, MM equation, and turnover number; Lineweaver - Burk plot,
Kinetics of Allosteric enzymes - MWC and KNF models Hill’ equation coefficient. Kinetics
of multi - substrate enzyme - catalysed reactions - Ping-pong bi-bi, random order and
compulsory order mechanism. Enzyme inhibition-Classification (a) competitive (b)
noncompetitive (c) uncompetitive (d) substrate
UNIT-III: MECHANISM OF ENZYME CATALYSIS (8 lectures )
Enzyme specificity, Mechanism of enzyme action - general acid-base catalysis, covalent
catalysis, proximity and orientation effects, role of metal ion in enzyme catalysis, mechanism
of serine proteases - chymotrypsin, lysozyme, and ribonuclease.
UNIT-IV: REGULATION (8 lectures )
Partial Proteolysis, Phosphorylation, adenylylation, disulphide reduction, Allosteric
regulation (1) sigmoidal kinetics (2) symmetry model (3) concerted model (4) kinetics and
functions of allosteric enzymes (a) phosphofructokinase (b) glycogen phosphorylase
UNIT-V: INDUSTRIAL USES OF ENZYMES (8 lectures )
Sources of industrial enzymes, thermophilic enzymes, amylases, glucose isomerases,
cellulose degrading enzymes, lipases, proteolytic enzymes in meat and leather industry,
detergents and cheese production. Clinical enzymology - Enzymes as thrombolytic agents,
antiinflammatory agents, digestive aids. Therapeutic use of asparginase, streptokinase.
Diagnostic enzymes. Immobilization of enzymes and their applications. Abzymes

References
1. Understanding enzymes: Palmer T., Ellis Harwood ltd., 2001.
2.Enzyme structure and mechanism. Alan Fersht, Freeman & Co. 1997
3. Principles of enzymology for food sciences: Whitaker Marc Dekker 1972.
4. Principles of Biochemistry, White. A, Handler, P and Smith.
5. Biochemistry, Lehninger A.L.
6. Biochemistry, Lubert Stryer.
7. Review of physiological chemistry, Harold A. Harper.
8. Text of Biochemistry, West and Todd.
 MOLECULAR BIOLOGY OF GENE

University- MGM University, Aurangabad Faculty- Engineering and Technology

Institute- Institute of Biosciences and Technology Degree- B.Tech. Biotechnology
Course Code- BTB- 123 Course Title- Molecular Biology of Gene
Credits- 3+0 (4Theory) Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 3hrs / weekly
Total Hours:
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester II
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form
Objective:
The course is an introduction to molecular biology and genetics and methods used within
thesefields. The subject content is the following.The structure of the genome: chromosomes,
chromosomal structure, and extrachromosomal inheritance. The molecular basis of
transmission of genetic information: nucleic acids and proteins. DNA replication, DNA
repair, mutations, recombination, transposition, transcription, and translation. Examples of
gene regulation.
Learning Outcomes:
After the course, the student should be able to:
1. account for structure, formation and function of DNA, RNA and proteins describe the
principles of gene regulation in prokaryotic and eukaryotic cells
2. describe the consequences of different types of mutations and DNA-repair systems,
recombination, restriction enzymes, operons and gene expression.
3. use some and understand several modern molecular methods to elucidate molecular
and genetic questions
4. critically analyse, evaluate and compile results from laboratory exercises reason about
ethical aspects of genetics

COURSE CONTENTS THEORY

UNIT I: DNA REPLICATION (8 lectures )
Prokaryotic and eukaryotic DNA replication – mechanism of replication, enzymes and
necessary proteins in DNA replication. DNA Mutation and Repair - mutation subtypes,
mismatch, base-excision and nucleotide excision. DNA recombination - homologous, non -
homologous and site-specific.
UNIT II: TRANSCRIPTION (8 lectures )
Prokaryotic and eukaryotic transcription - RNA polymerases - general and specific
transcription factors- regulatory elements. Mechanism of transcription regulation and
transcription termination. Post-transcriptional modification - 5’ cap formation- 3’end
processing and polyadenylation- splicing editing- nuclear export of mRNA- mRNA stability.
UNIT III: TRANSLATION (8 lectures )
Genetic code - Prokaryotic and eukaryotic translation - translational machinery. Mechanism
of initiation - elongation and termination. Regulation of translation.
UNIT IV: REGULATION OF ACTIVITY OF GENES AND GENE PRODUCTS IN
PROKARYOTES (8 lectures )
General aspects of Regulation, The lactose system and the operon model, The Galactose
operon, The Arabinose operon, The Tryptophan operon, Relative positions of Promoters and
Operators, Regulons, Regulation of Translation, Regulation of the synthesis of Ribosomes,
Unregulated changes in gene expression, Feedback Inhibition.
UNIT V: REGULATION IN EUKARYOTES (8 lectures )

Regulatory strategies in Eukaryotes, Gene alteration (Gene loss, Gene amplification, Gene
rearrangement: the joining of coding sequences in the immune system) Transcriptional
Control by hormones, Regulation mediate through Transcription factors, Regulation of
enhancer activity, Methylation, Regulation of processing, Translational control, Regulation
of gene expression in plant cells by light.

References:
1. Molecular Biology –David Frifelder
2. Genes IX –Benjamin Lewin
3. Molecular biology of the gene J. D. Watson and et. al.,
4. Molecular Biology of the Cell –Bruce Alberts and et. al.,
 IMMUNOLOGY

University- MGM University, Aurangabad Faculty- Engineering and Technology Institute-

Institute of Biosciences and Technology Degree- B.Tech. Biotechnology
Course Code- Course Title- Immunology
Credits- 2+0 (4 Theory) Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 2hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester II

Course Objective
1. Students should gain an understanding of basic aspects of the structure and functions of the
immune system.
2. Students should describe the applied aspects of immunology such as defense mechanism,
allergy and auto immunity.
3. Students should understand thecellular and molecular interaction of the immune responses.
Course Outcomes: The primary goal is to make Current understanding of the cellular and
molecular interactions in the inductions, expression, and regulation of the cellular and
humoral immune responses; recent knowledge and applications concerning immunity to
various microbial infections as well as antigen-antibody interactions; and detection of cell-
mediated immune response.

COURSE CONTENTS THEORY

UNIT I- IMMUNE SYSTEM (8 lectures )
Introduction to biology of the immune system, Organs and cells of immune system. Innate
and acquired immunity, Primary and Secondary lymphoid organs
UNIT II- ANTIGENS AND ANTIBODIES (8 lectures )
Nature of Antigen and Antibody, Antigen-Specificity, avidity, affinity, cross reactivity,
haptens, adjuvants, epitopes, Basic structure of antibodies ii) Classes of antibodies and
biological activity iii) Polyclonal antibodies, Antigen-antibody interactions – Principles and
applications
UNIT III- RECOGNITION OF ANTIGENS (8 lectures )
Major Histocompatibility Complex, Antigen processing and presentation, B- cell Maturation,
Activation and Differentiation, T- cell receptor – Structure and role, T- cell Maturation,
Activation and Differentiation
UNIT IV-IMMUNE EFFECTOR MECHANISMS (8 lectures )
Cytokines- - IL-1, IL-2, IL-4, IFNs and TNFs, Cytokine secretion by TH1 and TH2 cells,
Complement pathways- Classical, alternate and lectin pathways , Biological consequences of
complement activation, Complement fixation tests, Cell-mediated cytotoxicity of T cells, NK
cells, ADCC
UNIT V- ANTIGEN-ANTIBODY INTERACTIONS (8 lectures )
Antigen-antibody interactions –Principles and applications, Precipitation,
Immunoelectrophoresis, Agglutination, Radioimmunoassay, ELISA, Immunofluorescence,
Monoclonal antibodies (Hybridoma Technique).
Reference Books
1. Goldsby RA, Kindt TJ, Osborne BA. (2007). Kubyís Immunology. 6th edition W.H.
Freeman and Company, New York.
2.Richard C and Geiffrey S. (2009). Immunology. 6th edition. Wiley Blackwell
Publication
3.Cellular & Molecular Immunology, 7th Edition, Abul K. Abbas & Andrew H. Lichtman &
Shiv Pillai, 2011
 NUMERICAL AND COMPUTATIONAL FUNDAMENTALS I

University: MGM University, Aurangabad Faculty: Engineering and Technology Institute:

Institute of Biosciences and Technology. Degree: B. Tech-Biotechnology (UG)
Course Code- Course Title: Numerical and Computational
Fundamentals –I
Credits- 2+0 (4Theory) Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 2 hrs/weekly
Recommended Year /Semester: B. Tech-Biotechnology, Year 1/ Semester II
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form.
Course Objective:
1. To develop an understanding of basic mathematical tools and their application.
2. To model real world problems into mathematical expressions using numerical
representation.
Course Outcomes:
1. Use knowledge of content and mathematical procedures to solve problems.
2. Understand the fundamental ideas and application of Calculus.
COURSE CONTENTS
THEORY
UNIT I: Introduction: (8 Lectures)
Numbers, Strings, Lists, Loop, Functions, Data Structures, List, Tuples, Dictionaries,
Packages, pip,The dir() Function
UNIT II: Data analysis (8 Lectures)
Biostatistics, Data Input and Output, Formatted String Literals, The String format() Method
UNIT III: Machine Learning (8 Lectures)
Population studies, Distribution, ANOVA, Regression, multivariate analysis
UNIT IV: Quadratic Equations (8 Lectures)
Introduction, Quadratic equation, Factorization method, Completing square method, Formula
method, Nature of roots.
UNIT V: Permutation and Combination (8 Lectures)
Introduction, Factorial notation, Permutation, Combination.
Suggested Reading Book:
1.Class XI Mathematics, NCERT
2.Class X Mathematics, NCERT
 MENDEL AND DARWIN THEORY
University: MGM University, Aurangabad Faculty: Engineering and Technology Institute:
Institute of Biosciences and Technology. Degree: B. Tech- Biotechnology (UG)
Course Code- BTB-125 Course Title: Mendel and Darwin Theory
Credits allocated: 4+0 (Theory) Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 2 hrs/weekly
Recommended Year /Semester: B. Tech- Biotechnology (UG) Year 1/ Semester II

Prerequisites for registration: Registration of a student in various courses in consultation

with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form.
Course Objective:
1. To develop an understanding of basics of evolution and ideas of evolution.
2. To understand about the cause of heredity and variation.
Course Outcomes:
1. Use knowledge of genetics in the improvement of various traits.
2. Understand the fundamental ideas about the evolution

COURSE CONTENTS THEORY

UNIT I: Origin and Evolution of Life (8 Lectures)
Origin of Life, The meaning of evolution, Ideas of evolution before Darwin, Evidences of
Evolution, Theories of Evolution: Lamarck’s theory, Theory of germplasm, Darwin’s theory.
Modern views of Darwinism. Genetic basis of adaptation, Species concept.
UNIT II: Inheritance (8 Lectures)
Heredity and Variation, Genetics terms and symbol, Pre mendelian ideas of inheritance, John
Gregor Mendel, Theoroes of inheritance: Law of dominance, Law of seggregation and law of
Independent assortment. Rediscovery of Mendel’s work.
UNIT III: Traits and Alleles (8 Lectures)
Post mendelin era: other pattern of inheritance. Incomplete dominanace, Multiple alleles,
epistasis, polygenic traits, Pleotropy.
UNIT IV: Chromosomal Theory (8 Lectures)
Parallelism between genes and chromosomes, Chromosomal theory of inheritance, Linkage
and recombination
UNIT V: Chromosomal Variation (8 Lectures)
Genetic variation, Sex linked inheritance, Basics of sex determination, chromosomal
mapping.
Reference
1. Principles of Genetics:Robert H. Tamarin 7th ed. Tata McGraw Hill Publication. 2002.
2. Principles of Genetics: Gardener Simmons.
3. Genetics by Strictberger.
 MINI-PROJECT
University- MGM University, AURANGABAD Faculty- Engineering and Technology
Institute- Institute of Biosciences and Technology Degree B. Tech-BioTechnology (UG)
Course Code-BBL-128 Course Title- Micro project
Credits-0+2 Level of Study: UG
Mode of delivery planned learning activities and teaching method: Practical 4 hrs / weekly
Total Hours:
Recommended Year /Semester: B. Tech.. Biotechnology, Year 1/ Semester II

Prerequisites for registration: Registration of a student in various courses in consultation

with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form.
Objective- Students will get familiar to the topic in detail. They will have hands on different
instruments. They will develop technical handling skills.
Learning outcomes- Students will gain knowledge about need of research in particular field
and the idea development of how the lacuna can be erased that can be of social benefit.
COURSE CONTENTS
Practical
1. Synopsis
2. Project work
3. Thesis Writing
4. Presentation
 IMMUNO LAB
University- MGM University, Aurangabad Faculty- Engineering and Technology Institute-
Institute of Biosciences and Technology Degree- B.Tech. Biotechnology
Course Code- BBL-127 Course Title- Immuno –Enzymo Lab
Credits-0+1 Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 6 hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester II
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form.

Objective and Outcome: The students will be able to identify the cellular and molecular
basis of immune responsiveness. The students will be able to describe the roles of the
immune system in both maintaining health and contributing to disease.

COURSE CONTENTS
Practical
1. Blood staining.
2. Blood Grouping.
3. Rh factor detection .
4. Serum and plasma separation.
5. Radial immuno diffusion.
6. Antibiotics sensitive by paper disc Method .
7. Double diffusion.
8. Widal test.
9. To study immuno electro to detect Ag -Ab interaction.
10. Isolation of H and O Antigen from S.typhi.
11. Isolation of immunoglobulin by Ammonium sulphate precipitation.
12. Extraction and isolation of IgG ab from hen's egg.
13. Serum immunoelectrophorosis
14. Western blooting 15.
 COMPUTATIONAL LAB (Mol Bio)
University- MGM University, AURANGABAD Faculty- Engineering and Technology
Institute- Institute of Biosciences and Technology Degree- B.Tech. Biotechnology
Course Title- Computational Lab Course Code- BBL-127
Credits- 2+0 Level of Study:UG
Mode of delivery planned learning activities and teaching method: Lecture 6 hrs/weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester II
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form.

Objective and Outcome: To understand the classification & nomenclature of enzymes and
the factors effecting enzymatic reaction. The students will be able to identify the cellular and
molecular basis of immune responsiveness. The students will be able to describe the roles of
the immune system in both maintaining health and contributing to disease.

COURSE CONTENTS
Practical
1.Basic Electrical Engineering Practical
2.To identify different electrical measuring instruments
3.To measure voltage and current in electrical Circuit by voltmeter and ammeter.
4.To measure Power in electrical Circuit by Wattmeter
5.To Measure Electrical Energy in electrical circuit by Energy meter.
6.To determine Series equivalent of resistance
7.To determine parallel equivalent of resistance
8.Charging and discharging test on capacitor
9.To check insulation by Using insulation tester
10.Test circuit using series lamp and millimetre
11.To check earth resistance by Using the earth tester
12.For the given magnetic material find the B-H curve and hysteresis loop.
13.Use Faraday's first law of electromagnetic induction to analyses the behaviors of
statically induced E.M.F. and Dynamically induced E.M.F in the given circuit.

ENZYMO LAB
University- MGM University, Aurangabad Faculty- Engineering and Technology
Institute- Institute of Biosciences and Technology Degree- B.Tech. Biotechnology
Course Code- Course Title- Enzymo Lab
Credits-0+2 Level of Study: UG
Mode of delivery planned learning activities and teaching method: Lecture 6 hrs / weekly
Recommended Year /Semester: B.Tech. Biotechnology, Year 1/ Semester II
Prerequisites for registration: Registration of a student in various courses in consultation
with the respective course teacher and Adviser and acceptance by the Principal. The approved
courses must be mentioned in the roster form.

Objective and Outcome: To understand the classification & nomenclature of enzymes and
the factors effecting enzymatic reaction. The students will be able to identify the cellular and
molecular basis of immune responsiveness. The students will be able to describe the roles of
the immune system in both maintaining health and contributing to disease.

COURSE CONTENTS
Practical
1)Assay of amylase.
2)Assay of urease.
3)Assay of catalase .
4) Effect of pH,temp and substrate concentration on enzyme activity.
5) Estimation of glucose by DNS method.
6) Estimation of glucose by Benedictis trimetric method.
7) Estimation of total carbohydrate.
8)Test for lipids -Salkowski test,Lieberman-Burchard test.
9) Estimation of Amino Acid by Ninhydrin Method.
10) Estimation of protein by Biuret Method.
 List of Options to select from Bucket of Courses provided in various categories (Sample of Faculty of Basic and Applied
Sciences):

Major

Biotechnology

Food Processing
Technology
Minor options
Biomedical
Within Faculty of Basic Engineering
Sciences

Faculty of Faculty of Social

Faculty of Management Interdisciplinary
Engineering and Sciences and Faculty of Design Performing Arts
and Commerce Faculty
Technology Humanities

Minor
options Artificial Journalism and Mass Operations and Supply
Product Design Cosmetic Technology Theatre Arts
Intelligence (AI) Communication Management
from Other
Machine Learning Visual Educational
Faculty (ML)
Film Making
Communication
Human Resource (HR)
Technology
Dance

Data Analytics Photography Contemporary Arts Finance Management Yoga Sciences Music
Robotics Psychology Interior Design Marketing Physical Education Painting
Industrial
Economics Fashion Technology Accounting Library Sciences Pottery
Automation
 IKS (As per the UGC guidelines. Faculty of AEC (to be OE (Provide 4-8 Faculty of
Visit discussed and courses of your
Link:https://iksindia.org/English- developed by department to be
BGSamposhan-Kendram-1- committee of approved by the
updated.pdf) *** Dean)* BOS)
Holistic medicine and wellness Communicative Chemistry of Faculty of
English Biomolecules Biotechnology

Indian psychology and yoga Communication *Courses Instrumentation In Faculty of

***Courses and Soft Skills For Biotechnology Biotechnology
For reference reference
Indian sports and martial arts purpose only German purpose Introduction to Faculty of
only Fermentation Technology Biotechnology

Architectural engineering, town French Principles of Faculty of

planning, civil engineering, Vaastu and Developmental Biology Biotechnology
Shilpa Shastra
Sustainable agriculture and food Spanish
preservation methods

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VSEC Faculty of CC(Two courses to be VEC (to be discussed and developed by
(Respective finalized for I & II committee of Dean) ***
departments Semester)***
will prepare the
list)
NSS Universal Human Values
Digital Awareness
***Courses
Personality Development For Gandhian Studies
reference
Yoga purpose
only
NCC Value Education

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 ENTATIVE COURSE STRUCTURE III SEM TO VIII SEM

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Second Year (Semester III)
Teaching
period per Evaluation Scheme (Marks Minimum Passing (Marks)
week
Course Duration
Course code Course Title Type Credits
Type of Exam Internal External Internal
L T P Total Total

CA1 MSE CA2 TW ESE PR CA/MSE/TW ESE PR

PCC BBT42MML201 Microbe infection And 2 2 10 10 10 - 20 - 50 08 20
Theory
Immunity
PCC BBT42MML202 Genetics And Evolution Theory 2 - 2 10 10 10 - 20 - 50 08 20
PCC BBT42MML203 Numerical And Computational 2 2 10 10 10 - 20 - 50 08 20
Theory
Fundamentals-II
MDM Multidisplinary project Theory/Practical 2 - - 2 10 10 10 - 20 - 50 08 20
OE Open elective I Theory 2 2 10 10 10 - 20 - 50 08 20
OE Open elective II practical 4 2 30 20 - 50 08 20
AEC Ability Enhancement Course Theory 2 2 10 10 10 - 20 - 50 08 20
EEMC Theory/Practical 2 - 2 10 10 10 - 20 50 08 20
VEC Value Education course Theory 2 - 2 10 10 10 - 20 50 08 20
FP BBT42MMP201 Field project Practical 4 2 30 20 50 08 20
PCC BBT42MMP202 Genetics Lab Practical 4 2 30 20 50 08 20
Total = 28 16 12 22 650 260
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Second Year (Semester IV)
Teaching
period per Evaluation Scheme (Marks Minimum Passing (Marks)
Course week Duration of
Course code Course Title Type Credit Total Total
Type Exam Internal External Internal External
L T P CA
MSE CA2 TW ESE PR CA MSE TW ESE PR
1
PCC BBT42MML204 Recombinant DNA Technology Theory 2 2 10 10 10 - 20 - 50 08 20
PCC Genomics And Proteomics Theory 2 - 2 10 10 10 - 20 - 50 08 20
BBT42MML205
PCC BBT42MML206 Numerical And Computational Theory 2 2 10 10 10 - 20 - 50 08 20
Fundamentals-III
MDM Food Commodities Theory 2 - 2 10 10 10 - 20 - 50 08 20
OE Open elective I Theory 2 - 2 10 10 10 - 20 - 50 08 20
VSEC BBT42VSP201 Mini project Practical - 4 2 30 20 - 50 08 20
EEMC EEMC Theory 2 - 2 10 10 10 - 20 - 50 08 20
/Practical
VEC Value education course Theory 2 - - 2 10 10 10 - 20 - 50 20
PCC RDT & genomic lab - 4 2 30 20 - 50 08 20
BBT42FPJ203
PCC BBT42MMP204 Bio-Computational Lab Practical 4 2 - 30 - 20 50 08 20
PCC Indus Lab Practical 4 2 - 30 - 20 50 08 20
BBT42MMP205
Total = 30 14 16 22 550 220
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Third Year (Semester V)
Teaching
period per Evaluation Scheme (Marks Minimum Passing (Marks)
Course week Duration
Course code Course Title Type Credit
Type of Exam Internal External Internal External
L T P CA Total Total
MSE CA2 TW ESE PR CA MSE TW ESE PR
1
PCC Plant Tissue Engineering 3 3 20 20 20 - 40 - 100 16 40
BBT42MML301 Theory
PCC BBT42MML302 Cell Signalling Theory 3 - 3 20 20 20 - 40 - 100 16 40
PCC Numerical And Computational Fundamentals-IV Theory 2 2 10 10 10 - 20 - 50 08 20
BBT42MML303
MDM Food Safety And Microbial Standards Theory 2 - - 2 10 10 10 - 20 - 50 08 20
MDM Food safety Lab Practical - 4 2 30 20 - 50 08 20
PEC Program elective course I : : protein engineering Theory 2 2 10 10 10 - 20 - 50 08 20
and design
PEC Program elective course : project Project 4 2 30 20 - 50 08 20
/Practical
OE Open elective Theory 2 2 10 10 10 - 20 - 50 08 20
PCC Computational Lab Practical 4 2 30 20 50 08 20
BBT42MMP301
PCC BBT42MMP302 Plant Lab Practical 4 2 30 20 50 08 20
Total =30 14 16 22 600 240
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Third Year (Semester VI)
Teaching
period per Evaluation Scheme (Marks Minimum Passing (Marks)
Course week Duration of
Course code Course Title Type Credit
Type Exam
Internal External Internal External
L T P CA Total Total
MSE CA2 TW ESE PR CA MSE TW ESE PR
1
PCC 2 2 10 10 10 - 20 - 50 08 20
BBT42MML304 Python Theory

PCC BBT42MML305 2 2 10 10 10 - 20 - 50 08 20
Animal Tissue Engineering Theory

PCC 2 2 10 10 10 - 20 - 50 08 20
BBT42MML306 Molecular Cytogenetics Theory

PEC Programme Elective Course : Theory 3 3 20 20 20 - 40 - 100 16 40

Cancer cell biology
PEC Programme Elective Course Theory 3 3 20 20 20 - 40 - 100 16 40
:Nanobiotechnology
PEC Programme Elective Course: Practical 4 2 30 20 - 50 08 20
Nanobiotech Lab
MDM Bioethics ,Biosafety and Theory 2 - - 2 10 10 10 - 20 - 50 08 20
Regulatory Affairs
VSEC Micro Project Practical - 4 2 30 20 20 50 08 20
BBT42VSP301

PCC BBT42MMP303 4 2 30 20 50 08 20
Python Lab. Practical

PCC BBT42MMP304 Animal Tissue Lab Practical 4 2 30 20 50 08 20

Total = 30 14 16 22 600 240

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Fourth Year (Semester VII)
Teaching
period per Evaluation Scheme (Marks Minimum Passing (Marks)
Course week Duration
Course code Course Title Type Credit
Type of Exam Internal External Internal External
L T P CA Total Total
MSE CA2 TW ESE PR CA MSE TW ESE PR
1
Molecular Pharming And
PCC BBT42MML401 Theory 3 - 3 20 20 20 - 40 - 100 16 40
Biopharmaceuticals
BBT42MML402
PCC R language Theory 3 - 3 20 20 20 - 40 - 100 16 40

Advanced Topics in Molecular

PCC BBT42MML403 Theory 3 - 3 20 20 20 - 40 - 100 16 40
Biology
PCC BBT42MML404 Post Genomic Biology Theory 3 - 3 20 20 20 - 40 - 100 16 40
Program Elective Course : Gene,
PEC Theory 2 - 2 20 20 20 - 40 - 100 16 40
Marker And Omics Technology
Program Elective Course :Marine
PEC Theory 3 - 3 20 20 20 - 40 - 100 16 40
Bioprospecting
Program Elective Course
PEC Practical - - 4 2 20 20 20 - 40 - 100 16 40
Bioprospecting Lab
MDM Biological data analysis Theory 2 - 2 10 10 10 - 20 - 50 8 20
MDM Biological control system analysis Theory 2 - 2 20 20 20 - 40 - 100 16 40
Total = 25 21 4 23
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Fourth Year (Semester VIII)
Teaching
period per Evaluation Scheme (Marks Minimum Passing (Marks)
Course week Duration
Course code Course Title Type Credit
Type of Exam Internal External Internal External
L T P CA Total Total
MSE CA2 TW ESE PR CA MSE TW ESE PR
1
RM BBT42RML401 Research Methodology Theory 2 - - 2 20 20 20 - 40 - 100 16 40
RM BBT42RMP402 Research Methodology Practical - - 4 2 - - - 30 - 20 50 8 20
P BBT42PP401 Project Practical - - 8 4 - - - 30 - 20 50 8 20
OJT BBT42JTI401 On Job Training Internship 24 12
Total = 20
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