Scribd
Upload
94 views2 pages

Lineweaver Burk Plot

The document contains Lineweaver-Burk plots showing the effect of an inhibitor on the velocity of an enzyme-catalyzed reaction at different substrate concentrations. Data tables provide reaction velocities with and without the inhibitor, which are then plotted as 1/velocity vs 1/substrate concentration to obtain linear plots for calculating kinetic parameters.

Uploaded by

TAN YEE LING
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
94 views2 pages

Lineweaver Burk Plot

The document contains Lineweaver-Burk plots showing the effect of an inhibitor on the velocity of an enzyme-catalyzed reaction at different substrate concentrations. Data tables provide reaction velocities with and without the inhibitor, which are then plotted as 1/velocity vs 1/substrate concentration to obtain linear plots for calculating kinetic parameters.

Uploaded by

TAN YEE LING
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd
You are on page 1/ 2

1/ [S] 1/V

0.666667 4.761905
0.5 4
0.333333 3.571429
0.25 3.030303
0.125 2.272727
0.0625 2.5

[S] V Lineweaver-Burke plo


1.5 0.21 5
2 0.25 4.5 f(x) = 4.04919355706431 x + 2.04850851992526
3 0.28

1/ Velocity (min/µmole)
4
4 0.33 3.5
8 0.44 3
16 0.4 2.5
2
1.5
1
0.5
0
0 0.1 0.2 0.3 0.4

1/ [Sucrose] (1/mM)

without inhibitor with inhibitor


[S] V [S] V
1.5 0.21 1.5 0.08 Lineweaver-Burke plo
2 0.25 2 0.1 14
3 0.28 3 0.12
12
1/ Velocity (min/µmole)

4 0.33 4 0.13 f(x) = 11.109128375077 x + 4.80121005908081


8 0.44 8 0.16 10
16 0.4 16 0.18
8

1/ [S] 1/V 1/ [S] 1/V 6


0.666667 4.761905 0.666667 12.5
4 f(x) = 4.04919355706431 x + 2.04850851992526
0.5 4 0.5 10
0.333333 3.571429 0.333333 8.333333 2
0.25 3.030303 0.25 7.692308
0
0.125 2.272727 0.125 6.25 0 0.1 0.2 0.3 0.4 0.5
1/ [Sucrose] (1/mM)
0.0625 2.5 0.0625 5.555556
Lineweaver-Burke plot

9355706431 x + 2.04850851992526

0.2 0.3 0.4 0.5 0.6 0.7

1/ [Sucrose] (1/mM)

Lineweaver-Burke plot

x + 4.80121005908081

1 x + 2.04850851992526
without
inhibitor

0.3 0.4 0.5 0.6 0.7


1/ [Sucrose] (1/mM)

You might also like