B.SC Biotechnology (Hons) In-House CBCS - 2020

MAKAUT In-House B.Sc Biotechnology Hons.
Syllabus Structure [CBCS] 2020
CBCS – MAKAUT B.Sc Biotechnology B.Sc (Hons) 140 Credit FRAMEWORK

Subject Type and
Number of Subjects per Semester I Semester II Semester III Semester IV Semester V Semester VI
Semester
CORE COURSE 2 2 3 3 2 2
GENERIC ELECTIVE
1 1 1 1
SUBJECT
ABILITY
ENHANCEMENT 1 1
COMPULSORY
SKILL ENHANCEMENT
1 1
COURSE
DISCIPLINE SPECIFIC
2 2
ELECTIVE
Number of paper (total
4 (20) 5 (20) 5 (26) 5(26) 4 (24) 4 (24)
credit)
Total Credit- 20+26+26+20+24+24= 140
1|Page
MAKAUT In-House B.Sc Biotechnology Hons. Syllabus Structure [CBCS] 2020
Semester-I
CORE COURSE ABILITY ENHANCEMENT SKILL ENHANCEMENT GENERIC ELECTIVE DISCIPLINE SPECIFIC
(Credit=4+2) COMPULSORY COURSE SUBJECT ELECTIVE
( Credit =2) ( Credit =2)Any one ( Credit =6) (MOOCs) ( Credit =6) Any one from A
group and B group
Paper name Paper code Paper name Paper code Paper name Paper code Paper name Paper code Paper name Paper code
Biochemistry BSUBTC- English BSUBTA- MOOC Basket BSUBTG-1
and 101 Communication 101 1
Metabolism Skill MOOC Basket
(Theory) Development 2
Biochemistry BSUBTC- MOOC Basket
and 191 3
Metabolism MOOC Basket
(Lab) 4
Cell Biology BSUBTC-
(Theory) 102
Cell Biology ( BSUBTC-
Lab ) 192
Credit- 6+6=12 Credit- 2 Credit- 6
Total Credit- 12+2+6=20
2|Page
Semester-II
( Credit =2)Any one ( Credit =2)Any one ( Credit =6)(MOOCs) ( Credit =6) Any one from A
group and B group
Paper name Paper code Paper name Paper code Paper name Paper Paper name Paper code Paper name Paper code
code
General BSUBTC- Introduction to BSUBTA- MOOC Basket 1 BSUBTG-2
microbiology 201 Environmental 201 MOOC Basket 2
(Theory) Science MOOC Basket 3
General BSUBTC- Introduction to BSUBTA- MOOC Basket 4
microbiology 291 fundamental 202
( Lab) computer
Plant and BSUBTC-
Mammalian 202
Physiology
(Theory)
Plant and BSUBTC-
Mammalian 292
Physiology (
Lab )
Credit- 6+6=12 Credit- 2 Credit- 6
3|Page
Semester-III
CORE COURSE ABILITY SKILL ENHANCEMENT GENERIC ELECTIVE DISCIPLINE SPECIFIC

(Credit=4+2) ENHANCEMENT COURSE SUBJECT ELECTIVE
COMPULSORY ( Credit =2)Any one ( Credit =6)(MOOCs) ( Credit =6) Any one from A
( Credit =2)Any one group and B group
Paper name Paper code Paper name Paper Paper name Paper code Paper name Paper code Paper name Paper code
code
Genetics BSUBTC- Enzymology BSUBTS- MOOC Basket 1 BSUBTG-3
(Theory) 301 301 MOOC Basket 2
Genetics ( BSUBTC- Industrial BSUBTS- MOOC Basket 3
Lab ) 391 Biotechnology 302 MOOC Basket 4
Chemistry I BSUBTC- Plant and animal BSUBTS-
(Theory) 302 chromosome 303
preparation and
karyotyping.
Chemistry I BSUBTC-
( Lab ) 392
Molecular BSUBTC-
Biology 303
(Theory)
Molecular BSUBTC-
Biology ( 393
Lab )
Credit- 6+6+6=18 Credit- 2 Credit- 6
4|Page
Semester-IV
( Credit =2)Any one ( Credit =2)Any one ( Credit =6)(MOOCs) ( Credit =6) Any one from A
group and B group
Paper name Paper Paper name Paper Paper name Paper code Paper name Paper code Paper name Paper code
code code
Immunology BSUBTC Molecular BSUBTS MOOC Basket 1 BSUBTG-4
(Theory) -401 Diagnostics -401 MOOC Basket 2
Immunology ( BSUBTC Plant-Microbe BSUBTS- MOOC Basket 3
Lab ) -491 Interaction 402 MOOC Basket 4
Chemistry2 BSUBTC Research BSUBTS-
(Theory) -402 Methodology 403
Chemistry 2 ( BSUBTC Basic Forensic BSUBTS-
Lab ) -492 Science 404
Bioanalytical BSUBTC
tools -403
(Theory)
Bioanalytical BSUBTC
tools ( Lab ) -493
Credit- 6+6+6=18 Credit- 2 Credit- 6
5|Page
Semester-V
( Credit =2)Any one ( Credit =2)Any one ( Credit =6) ( Credit =6) Any one from A
(MOOCs) group and B group
Bioprocess BSUBTC- Animal BSUBTD-
Technology 501 Biotechnology 501A
(Theory)
Bioprocess BSUBTC- Model organism BSUBTD-
Technology ( 591 and human 502A
Lab ) genome project
Recombinant BSUBTC- Medical BSUBTD-
DNA 502 biotechnology 503A
Technology
(Theory)
Recombinant BSUBTC- Plant BSUBTD-
DNA 592 Biotechnology 501B
Technology (
Lab )
Plant secondary BSUBTD-
metabolites and 502B
Biotransformation
Credit- 6+6=12 Credit- 6+6=12
Total Credit- 12+12=24
6|Page
Semester-VI
( Credit =2)Any one ( Credit =2)Any one ( Credit =6)Any one ( Credit =6) Any one from A
(MOOCs) group and B group
Genomics, BSUBTC- Genetic BSUBTD-
Proteomics 601 Modification In 601A
and agriculture, food
Bioinforma and medicine
tics(Theory)
Genomics, BSUBTC- Environmental BSUBTD-
Proteomics 691 Biotechnology 602A
and
Bioinforma
tics( Lab )
IPR, Biosafety BSUBTC- Project/ BSUBTD-
and ethical 602 Dissertation 681B
issues(Theory)
IPR, Biosafety BSUBTC-
and ethical 692
issues( Lab )
Credit- 6+6=12 Credit- 6+6=12
Total Credit- 12+12=24
7|Page
Credits point distribution per Semester:

Core Course [ Ability Skill Generic Discipline Semester
Credit=4+2] enhancement Enhancement elective Specific Specific Total
Compulsory Course Subject Subject Credit
[ Credit=2] [ Credit=2] {MOOC} [ Credit=6]
[ Credit=6]
SEM-I [4+2]+[4+2] 2 0 6 0 20
SEM-II [4+2]+[4+2] 2 0 6 0 20
SEM-III [4+2]+[4+2]+ [4+2] 0 2 6 0 26
SEM-IV [4+2]+[4+2]+ [4+2] 0 2 6 0 26
SEM-V [4+2]+[4+2] 0 0 0 6+6 24
SEM-VI [4+2]+[4+2] 0 0 0 6+6 24
Course 84 4 4 24 24 140 is the Total
Specific Credit for B.Sc
Total Credit Biotechnology
Course
8|Page
MOOCs Basket
MOOC Basket 1 MOOC Basket 2 MOOC Basket 3 MOOC Basket 4
HUMANITIES & HUMAN CREATIVE & GENERAL SCIENCE EMERGING TECH,

SKILLS PERFORMING ARTS INNOVATION &
ENTREPRENEURSHIP
1. Sports for All 11. Drama &Theatre 20. Renewable Energy 34. Creativity and
2. Wellness Lifestyle Arts 21. Biotechnology and Innovation
3. Yoga and Stress 12. Film Video & Human Welfare 35. Entrepreneurship
Management Photographic Arts 22. Environmental Theory and Practice
4. Understanding 13. Music-Indian, Biotechnology 36. Introduction to
Human Rights and Western & Fusion 23. Principles of Modern Mobile Application
Responsibility 14. Event Management Physics Development
5. Indian Society and 15. Digital Productions 24. Introduction to 37. E-Commerce System
Culture 16. Set Design Biological Physics Development
6. Fundamental 17. Multimedia 25. Biological Basis of 38. Ethical Hacking and
Psychological 18. AR-VR Behaviour Intrusion Forensics
Process 19. Scientific Illustration 26. Mathematics 39. Business Statistics
7. Industry 4.0 27. Statistics and Research
8. Leadership Skill 28. The Yoga Methods
9. Business English Professional 40. Data Science
10. Health Education & 29. History of Biology 41. Data Security
Communication 30. Structural Biology 42. Big Data
31. Microscopy, 43. C++, Pithon & R
spectroscopy 44. Internet of Things
32. Inheritance Biology 45. Data structure and
33. Biodiversity, Numerical analysis
Taxonomy 46. DBMS and
Computer
networking
9|Page
B.Sc Biotechnology Syllabus
B.Sc Biotechnology
Syllabus
(2020)
Department of
Biotechnology
MaulanaAbulKalam Azad
University of Technology
(MAKAUT)
1
SEMESTER-I
BSUBTC-101 Biochemistry and MetabolismCredit- 4
UNIT I:
Introduction to Biochemistry: A historical prospective.
Carbohydrates:- Structural aspects – Introduction & Occurrence, Classification of Mono-, Di- and
Polysaccharides, Reducing & Non-reducing Sugars, Constitution of Glucose & Fructose, Osazone
formation, Pyranose& Furanose forms, Determination of ring size, Inter-conversion of
monosaccharides.
Amino acids & Proteins: Structure & Function. Structure and properties of Amino acids, Types of
proteins and their classification, Forces stabilizing protein structure and shape. Different Level of
structural organization of proteins, Protein Purification. Denaturation and renaturation of proteins.
Fibrous and globular proteins.Protein misfolding and related diseases.
UNIT II
Lipids: Structural aspects – General introduction, Classification & Structure of Simple & Compound
lipids, Properties of Lipid aggregates (elementary idea), Biological membrane, membrane protein –
structural aspects, Lipoproteins (elementary idea).Membrane Transport.
Nucleic acids: Structure and functions: Physical & chemical properties of Nucleic acids, Nucleosides
& Nucleotides, purines & pyrimidines,. Biologically important nucleotides, Double helical model of
DNA structure and forces responsible for A, B & Z – DNA, denaturation and renaturation of DNA
UNIT-III
Chemical & Enzymatic Kinetics - An introduction to enzyme; How enzyme works; Reaction rate;
Thermodynamic definitions; Principles of catalytic power and specificity of enzymes, Order of
reactions; Enzyme kinetics – Approach to mechanism.
UNIT IV
Carbohydrates Metabolism: Reactions, energetics and regulation. Glycolysis: Fate of pyruvate under
aerobic and anaerobic conditions. Pentose phosphate pathway and its significance,
Gluconeogenesis, Glycogenolysis and glycogen synthesis. TCA cycle, Electron Transport Chain,
Oxidative phosphorylation. ß-oxidation of fatty acids.
Lipid Metabolism – Structures and roles of Fatty acids &Glycerols, beta oxidation of saturated fatty
acids, oxidation of unsaturated fatty acids, oxidation of odd chain fatty acids, energy yield, Ketone
bodies.
Amino acid Metabolism – Amino acid breakdown (amino acid deamination, Urea cycle, metabolic
breakdown of individual amino acids – glucogenic & ketogenic amino acids), amino acids as
biosynthetic precursors (haem biosynthesis & degradation, biosynthesis of epinephrine, dopamine,
seretonin, GABA, histamin, glutathione); biosynthesis of essential & non-essential amino acids.
Nucleotide Metabolism – biosynthesis of purine & pyrimidine (de novo& salvage pathway);
degradation of purine & pyrimidine.
BSUBTC-191 (Lab) [Lab on Biochemistry and Metabolism]Credit 2

(Wherever wet lab experiments are not possible the principles and concepts can be demonstrated
through any other material or medium including videos/virtual labs etc.)
1. Estimation of protein by Folin Lowry method
2. TLC separation of Amino acids /sugars
3. Determination of Iodine number of a fat
4. Estimation of RNA by Orcinol method
5. Estimation of DNA by diphenyl amine method
6. Qualitative tests for Carbohydrates, lipids and proteins
7. Testing of Blood Sugar
2
8. Testing of Liver Function Test (Bilirubin, SGOT, SGPT, Alkaline Phosphatase, Albumin,
Globulin, Total Protein)
9. Testing of Renal Function Test (Urea, Uric acid, Creatine, Creatinine)
BSUBTC-102 Cell Biology Credit 4
UNIT I
Basics of Cell Biology (structure & function) – Discovery of cell and Cell Theory; Comparison
between plant and animal cells; cytosol, compartmentalization of eukaryotic cells, cell
fractionation.Cell Membrane and Permeability: Chemical components of biological membranes,
organization and Fluid Mosaic Model, membrane as a dynamic entity, cell recognition and membrane
transport. Cell wall; Plasma membrane; Modification of plasma membrane and intracellular
junctions; Cytoskeleton; Protoplasm; Mitochondria; Chloroplast; ER; Golgi complex;
UNIT II
Membrane Vacuolar system, cytoskeleton and cell motility: Structure and function of microtubules,
Microfilaments, Intermediate filaments. Endoplasmic reticulum: Structure, function including role in
protein segregation. Golgi complex: Structure, biogenesis and functions including role in protein
secretion.
UNIT III
Lysosomes: Vacuoles and micro bodies: Structure and functions Ribosomes: Structures and function
including role in protein synthesis. Mitochondria: Structure and function, Genomes, biogenesis.
Chloroplasts: Structure and function, genomes, biogenesis Nucleus: Structure and function,
chromosomes and their structure.
UNIT IV
Extracellular Matrix: Composition, molecules that mediate cell adhesion, membrane receptors for
extra cellular matrix, macromolecules, regulation of receptor expression and function. Signal
transduction.
Cell cycle - An overview of cell cycle; Components of cell cycle control system; Intracellular and
Extra- cellular control of cell division, Programmed cell death (Apoptosis), intrinsic & extrinsic
pathways of cell death, Apoptosis in relation with Cancer,
Cancer: Carcinogenesis, agents promoting carcinogenesis, characteristics and molecular basis of
cancer.
BSUBTC-192 (Lab) )[Lab on Cell Biology] Credit 2
1. Preparation of Mitotic Chromosome from onion root tip.

3. Preparation of Meiotic Chromosome from Rhoeo discolor or onion sp.
4. Preparation and study of polytene chromosome from Drosophila salivary gland.
5. Study of sex chromatin through preparation of Barr body from buccal epithelium .
6. Study of chromosomal aberration induced by pesticide in onion root tips.
7. Study of plasmolysis and de-plasmolysis.
Learning Resources:
1. Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman
and Co.
2. Buchanan, B., Gruissem, W. and Jones, R. (2000) Biochemistry and Molecular Biology of
Plants.American Society of Plant Biologists.
3. Nelson, D.L., Cox, M.M. (2004) Lehninger Principles of Biochemistry, 4th Edition, WH
3
Freeman and Company, New York, USA.

4. Hopkins, W.G. and Huner, P.A. (2008) Introduction to Plant Physiology. John Wiley and
Sons.
5. Salisbury, F.B. and Ross, C.W. (1991) Plant Physiology, Wadsworth Publishing Co. Ltd.
BSUBTA-101 English Communication Skill development Credit2
1. Communication and communicative activities of the notions of encoder and decoder and the
message and the medium.
2. Concise grammatical structures and key vocabulary for general as well as specific purpose
accuracy and appropriateness in the use of English.
3. English speech sounds and sound combinations.
4. Elements of Spoken English.
5. Topic of discourse, mode of discourse and style of discourse with special reference to
scientific discourse.
6. Writing notes, reports, proceedings etc.
7. Expanding and summarizing.
8. Narrating and describing.
9. Tutorial for each topic.
BSUBTG-1(1,2,3,….n): MOOCS Credit: 6
MOOC Basket 1
MOOC Basket 2
MOOC Basket 3
MOOC Basket 4
4
SEMESTER-II
BSUBTC-201 General Microbiology Credit 4
UNIT I
Overview of history of Microbiology - Biogenesis and abiogenesis Contributions of Redi,
Spallanzani, Needham, Pasteur, Tyndal, Joseph Lister, Koch [Germ Theory], Edward Jenner and
Flemming [Penicillin], Scope of Microbiology.
Classification of Microbes - Systems of classification, Numerical taxonomy, Identifying characters for
classification, General properties and principles of classification of microorganisms Systematics of
bacteria, General properties of Archae and Eubacteria.
UNIT II
Staining: Concept of auxochrome, chromophore, dyes, Mechanism of gram staining, acid fast
staining, negative staining, capsule staining, flagella and endospore staining.
UNIT III
Methods of isolation: Cultivation and Maintenance of microorganisms, Concept of Sterilization -
Definition of sterilization, dry and moist heat, pasteurization, tyndalization; radiation, ultrasonication,
filtration. Physical and Chemical methods of sterilization; disinfection sanitization, antisepsis
sterilants and fumigation. Determination of phenol coefficient of disinfectant, Chemotherapeutic
agents.
UNIT III
Microbial growth: Growth curve, Generation time, synchronous batch and continuous culture,
measurement of growth and factors affecting growth of bacteria. Nutritional types [Definition and
examples]. Classification on the basis of oxygen requirement.
Microbial Metabolism: Metabolic pathways, amphi-catabolic and biosynthetic pathways Bacterial
Reproduction: Transformation, Transduction and Conjugation. Endospores and sporulation in
bacteria.
UNIT IV
Water Microbiology: Bacterial pollutants of water, coliforms and non coliforms. Sewage composition
and its disposal.
Food Microbiology: Important microorganism in food Microbiology: Moulds, Yeasts,
bacteria.Major food born infections and intoxications, Preservation of various types of foods.
Fermented Foods ( Yoghurt, cheese, Idli, Kinema).
BSUBTC-291 (Lab)[Lab on General Microbiology] Credit 2
1. Sampling and quantification of microorganisms in air, soil and water.
2. Isolation of bacteria [Streak plate, spread plate, pour plate,serial dilution].
3. Identification of microorganisms from the habitats [simple staining,differential staining, acid
fast staining, capsule staining, spore staining and motility]
4. Observation of morphology - shape and arrangement of cells.
5. Methods of inoculation of different microbes in selective media.
6. Microscopic measurements, micrometer (ocular and stage).
7. Enumeration of microorganism - total & viable count.
Learning Resources:-
1. Alexopoulos CJ, Mims CW, and Blackwell M. (1996). Introductory Mycology. 4
5
thedition.ohn and Sons, Inc.

2. Jay JM, Loessner MJ and Golden DA. (2005). Modern Food Microbiology. 7thedition, CBS
Publishers and Distributors, Delhi, India.
3. Kumar HD. (1990). Introductory Phycology. 2nd edition. Affiliated East Western Press.
4. Madigan MT, Martinko JM and Parker J. (2009). Brock Biology of Microorganisms. 12th
edition. Pearson/Benjamin Cummings.
5. Pelczar MJ, Chan ECS and Krieg NR. (1993). Microbiology. 5th edition. McGraw Hill Book
Company.
6. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General Microbiology. 5th
edition. McMillan.
7. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9 th edition.
Pearson Education.
8. Willey JM, Sherwood LM, and Woolverton CJ. (2008). Prescott, Harley and Klein’s
Microbiology. 7th edition. McGraw Hill Higher Education.
BSUBTC-202 Plant and Mammalian Physiology Credit 4
UNIT IA
Digestion and Respiration
Digestion: Mechanism of digestion & absorption of carbohydrates, Proteins, Lipids and nucleic acids.
Respiration: Exchange of gases, Transport of O2 and CO2, Oxygen dissociation curve, Chloride shift.
UNIT IB
Simple & complex permanent tissues, primary structure of shoot & root, secondary growth, growth
rings, leaf anatomy (dorsi-ventral and isobilateral leaf)
UNIT IIA
Circulation
Composition of blood, Plasma proteins & their role, blood cells, Haemopoisis, Mechanism of
Coagulation of blood.Mechanism of working of heart: Cardiac output, cardiac cycle, Origin &
conduction of heart beat.
UNIT IIB
Plant water relations and micro & macro nutrients
Importance of water to plant life, diffusion, osmosis, plasmolysis, imbibition, guttation, transpiration,
stomata & their mechanism of opening &closing.Micro& macro nutrients: criteria for identification of
essentiality of nutrients, roles anddeficiency systems of nutrients, mechanism of uptake of nutrients,
mechanism of food transport.
UNIT IIIA
Muscle physiology and osmoregulation
Structure of cardiac, smooth & skeletal muscle, threshold stimulus, single muscle twitch, muscle tone,
isotonic and isometric contraction, Physical, chemical & electrical events of mechanism of muscle
contraction.
Excretion: modes of excretion, Ornithine cycle, Mechanism of urine formation
UNIT IIIB
Carbon and nitrogen metabolism
CO2 Capture, Photosynthesis- Photosynthesis pigments, concept of two photo systems,
photphosphorylation, calvin cycle, CAM plants, photorespiration, compensation point Nitrogen
metabolism- inorganic & molecular nitrogen fixation, nitrate reduction and ammonium assimilation in
plants.
6
UNIT IVA
Nervous and endocrine coordination
Mechanism of generation & propagation of nerve impulse, structure of synapse, synaptic conduction,
saltatory conduction, Neurotransmitter Mechanism of action of hormones (insulin and
steroids)Different endocrine glands– Hypothalamus, pituitary, pineal, thymus, thyroid, parathyroid
and adrenals, hypo & hyper-secretions.
UNIT IVB
Growth and development
Definitions, phases of growth, growth curve, growth hormones (auxins, gibberlins, cytokinins,
abscisic acid, ethylene) Physiological role and mode of action, seed dormancy and seed germination,
concept of photoperiodism and vernalization.
BSUBTC-292 (Lab)[Lab on Plant and mammalian Physiology] Credit 2
1. Finding the coagulation time of blood

2. Determination of blood groups
3. Counting of mammalian RBCs
4. Determination ofHaemoglobin
5. Preparation of stained mounts of anatomy of monocot and dicot root and stem.
6. Separation of photosynthetic pigments by paper chromatography.
7. Demonstration of aerobic respiration and photosynthesis
LEARNING RESOURCES
1. Guyton, A.C. & Hall, J.E. (2006). Textbook of Medical Physiology. XI Edition. Hercourt
Asia PTE Ltd. /W.B. Saunders Company.
2. Tortora, G.J. & Grabowski, S. (2006). Principles of Anatomy & Physiology. XI Edition. John
wiley&sons,Inc.
3. Dickinson, W.C. 2000 Integrative Plant Anatomy. Harcourt Academic Press, USA.
4. Esau, K. 1977 Anatomy of Seed Plants. Wiley Publishers.
5. Fahn, A. 1974 Plant Anatomy. Pergmon Press, USA and UK.
6. Hopkins, W.G. and Huner, P.A. 2008 Introduction to Plant Physiology. John Wiley and Sons.
7. Mauseth, J.D. 1988 Plant Anatomy. The Benjammin/Cummings Publisher, USA.
8. elson, D.L., Cox, M.M. 2004 Lehninger Principles of Biochemistry, 4th edition, W.H.
Freeman and Company, New York, USA.
9. Salisbury, F.B. and Ross, C.W. 1991 Plant Physiology, Wadsworth Publishing Co. Ltd.
10. Taiz, L. and Zeiger, E. 2006 Plant Physiology, 4thedition, Sinauer Associates Inc .MA, USA.
BSUBTA-201: Introduction to Environmental Science Credit2
UNIT I
Introduction to environmental studies & ecosystems: Multidisciplinary nature of environmental
studies: Scope and importance; what is an ecosystem? The structure and function of ecosystem,
Energy flow in an ecosystem, food chains, food webs and ecological succession, forest ecosystem,
grassland ecosystem, desert ecosystem, aquatic ecosystems; Levels of biological diversity such as
genetic, species and ecosystem diversity; biogeography zones of India, biodiversity patterns and
global biodiversity hot spots, India as a mega-biodiversity nation, endangered and endemic species of
7
India, threats to biodiversity, habitat loss, poaching of wildlife, man-wildlife conflicts, biological
invasions, conservation of biodiversity, in-situ and ex-situ conservation of biodiversity, concept of
sustainability and sustainable development.
UNIT II
Natural resources & its management and conservation: Land resources and land use change: Land
degradation, soil erosion and desertification; Deforestation: Causes and impacts due to mining, dam
building on environment, forests, biodiversity and tribal populations; Water: Use and over-
exploitation of surface and ground water, floods, droughts, conflicts over water (international & inter-
state); Energy resources: Renewable and non renewable energy sources, use of alternate energy
sources and growing energy needs.
UNIT III
Environmental pollution & management: Environmental pollution: types, causes, effects and
controls; Air, water, soil and noise pollution, Solid waste management: Control measures of urban and
industrial waste. Climate change, global warming, ozone layer depletion, acid rain and their impact on
human communities and agriculture. Environment Laws: Environment Protection
Act, Air (Prevention & Control of Pollution) Act, Water (Prevention and control of pollution) Act,
Wildlife Protection Act, Forest Conservation Act; International agreements: Montreal and Kyoto
protocols and Convention on Biological Diversity (CBD); Nature reserves, tribal populations and
rights, and human wildlife conflicts in Indian context.
UNIT IV
Environment & social issues: Human population growth: Impacts on environment, human health and
welfare; Resettlement and rehabilitation of project affected persons; case studies; Disaster
management: floods, earthquake, cyclones and landslides; Environmental movements: Chipko, Silent
valley, Bishnois of Rajasthan; Environmental ethics: Role of Indian and other religions and cultures
in environmental conservation; environmental communication and public awareness.
Learning resources
1. Carson, R. 2002. Silent Spring. Houghton Mifflin Harcourt.

2. Gadgil, M., & Guha, R. 1993. This Fissured Land: An Ecological History of India. Univ. of
California Press.
3. Gleeson, B. and Low, N . (eds.) 1999. Global Ethics and Environment, London, Routledge.
4. Gleick, P. H. 1993. Water in Crisis. Pacific Institute for Studies in Dev., Environment &
Security. Stockholm Env. Institute, Oxford Univ. Press.
5. Groom, Martha J., Gary K. Meffe, and Carl Ronald Carroll. Principles of Conservation
Biology. Sunderland: Sinauer Associates, 2006.
6. Grumbine, R. Edward, and Pandit, M.K. 2013. Threats from India’s Himalaya dams. Science,
339: 36-37.
7. McCully, P. 1996. Rivers no more: the environmental effects of dams (pp. 29-64). Zed
Books.
8. Pepper, I.L., Gerba, C.P. &Brusseau, M.L. 2011. Environmental and Pollution Science.
Academic Press.
9. Rao, M.N. & Datta, A.K. 1987. Waste Water Treatment. Oxford and IBH Publishing Co. Pvt.
Ltd.
10. Raven, P.H., Hassenzahl, D.M. & Berg, L.R. 2012. Environment. 8th edition. John Wiley &
Sons.
11. Rosencranz, A., Divan, S., & Noble, M. L. 2001. Environmental law and policy in India.
Tripathi 1992.
12. Sengupta, R. 2003. Ecology and economics: An approach to sustainable development. OUP.
13. Singh, J.S., Singh, S.P. and Gupta, S.R. 2014. Ecology, Environmental Science and
14. CSthooednThsrei,orvpNai.ctSiso.., n JG.oShibn. sCWohnai,lneLdy. P&&u bSRloiasnvhsei.
nn g,
,PN.Hew. ( Dedesl)h.i .2 013. Conservation Biology: Voices from
8
15. Wilson, E. O. 2006. The Creation: An appeal to save life on earth. New York: Norton.
16. World Commission on Environment and Development. 1987. Our Common Future. Oxford
University Press.
BSUBTA-202 Introduction to Fundamental Computer Credit-2

Unit -I
Generation of computer: 1st to 4th generation with their characteristics .Basic concept of computer:
Introduction, different components of computer, basic design of computer.
Unit-II
Introduction to operating system, Introduction to OS, different management (processor, memory,
device, file),
Unit-III
Processor management-Process concept, Threads, CPU Scheduling Process scheduling, Deadlocks,
Process synchronization.
Unit-IV
Memory management –Memory allocation rule, Swapping, Overlay, Paging, Demand paging
,segmentation ,virtual memory. Device management, File management.
Reference Books:
• Computer Fundamentals – by Pradeep K Sinha, Priti Sinha
• Operating System Concepts – by Abraham Silberschatz, Peter B. Galvin, Gerg Gange
• Operating System – by P. Bala Krishna Prasad
• Digital Design - by M. Morris R. Mano (Author), Michael D. Ciletti (Author)
• Digital Logic and Computer Design – by M. Morris Mano.
BSUBTG-2(1,2,3,….n): MOOCS Credit: 6
MOOC Basket 1
MOOC Basket 2
MOOC Basket 3
MOOC Basket 4
9
SEMESTER-III
BSUBTC- 301 Genetics Credit 4
UNIT I
Prokaryotic Genomes - Physical organization of bacterial genomes (Structure of the bacterialnucleoid,
Replication and partitioning of the bacterial genome and Genome of Archaea).Mechanism of genetic
exchange: Plasmid and bacterial sex, Types of plasmids (F Plasmid : a Conjugate plasmid’,
Mobilization of Non-conjugative plasmid, R plasmid, Col plasmid Copy number and incompatibility),
Episomes.
Transcriptional regulation in prokaryotes (inducible and repressible system, positive regulation and
negative regulation); Operon concept – lac, trp, Ara operons. Transduction (Generalized transduction,
Specialized Transduction)- gene mapping.
UNIT II
Genome organisation and Fine structure of the Gene: Genes and Gene numbers, C value paradox,
Denaturation and Renaturation of DNA- Tm values and Cot curves, Repetitive and non-repetitive
DNA, Inverted and Tandem repeats, Satellite DNA, Gene clusters-Histone, rRNA
Eukaryotic Chromosome- Macro-molecular organization. Primary and Secondary constriction, Sat-
bodies, telomeres. Heterochromatin and Euchromatin and its significance. Ultrastructureof
chromosome- Nucleosome model and Nucleosome Structure.
UNIT III
Unique genetic features of plants - Ability to photosynthesize, Totipotency of plant cells,
Hermaphroditism and ability to reproduce both sexually and asexually, Doublefertilization,
Alternation of generations, Mitosis in haploid state.
Genes controlling flower development in Plants – genes responsible for steps of flower development,
genes for floral organ identity, MADS-Box genes, molecular expression of floral organ genes
andfloral commitment genes.
Genome Organization and Function - Analysis of Genomes by Re-association
Experiments,Organization of Single-copy Sequences, Chloroplast Genome Organization,
Mitochondrial Genome Organization, RNA editing.
Cis-acting elements and Trans-acting factors – Regulatory sequences that control gene expression,
Enhancer and Silencer elements, role of 3’ sequences, role of introns, conserved sequences in
Eukaryotic promoters, Cis-acting elements, Trans-acting factors, Transposon tagging of Plant genes –
McClintock and the Ac-Ds transposable elements of Corn,
UNIT IV
Chromosomal variation in Number & Structure– Euploidy, Non-disjunction & Aneuploidy,
Aneuploid segregation in plants and animal, Polyploidy in Plants & Animals, Induced Polyploidy,
applications of Polyploidy, Chromosomal Mosaics, Polytene chromosome in Diptera, structural
chromosomal variation, Chromosomal aberrations & evolution.
Human Cyto-Genetics– Human karyotype, Banding techniques, classification, use of Human Cyto-
genetics in Medical science, , viable monosomies&trisomies, chromosomal deletions & duplications,
genetics of chromosomal inversions & translocations, human traits.
BSUBTC-391: (Lab) [Lab on Genetics] Credit 2
1. Permanent and temporary mount of mitosis.
2. Permanent and temporary mount of meiosis.
3. Problems based on Reassociation Kinetics
10
4. Karyotyping with the help of photographs.
Learning Resources
1. Gardner, E.J., Simmons, M.J., Snustad, D.P. (2006). Principles of Genetics. VIII Edition John
Wiley & Sons.
2. Snustad, D.P., Simmons, M.J. (2009). Principles of Genetics. V Edition. John Wiley and Sons
Inc.
3. Klug, W.S., Cummings, M.R., Spencer, C.A. (2009). Concepts of Genetics. IX Edition.
Benjamin Cummings.
4. Russell, P. J. (2009). Genetics- A Molecular Approach. III Edition. Benjamin Cummings.
5. Griffiths, A.J.F., Wessler, S.R., Lewontin, R.C. and Carroll, S.B. IX Edition. Introduction to
Genetic Analysis, W. H. Freeman & Co.4. Theory and problems in Genetics by Stansfield.
BSUBTC-302 Chemistry I Credit 4
Atomic Structure, radioactivity and Nuclear Structure of Atoms:

Bohr,s atomic model & limitation. Idea of de Broglie matter weaves. Hisenberg’suncertaintyprinciple.
Schrodinger’s wave equation. Significance of wave function. Quantum numbers. Multielectron
system- Pauli’s exclusion principal, Hunds rules of maximum multiplicity. Stability of half filled and
full field orbitals, Afbau principal & its limitation. Electronic configuration of atoms.
Radioactive disintegration series, group displacement law, law of radioactive decay, half-life and
average life of radio elements, radioactive equilibrium, measurement of radioactivity. Stability of
atomic nucleus, n/p ratio. Radioisotopes and their application: Determination of age of earth, radio
carbon dating, Medicinal and agriculture use of isotopes, hazards of radio activity.
Chemical Bonding and Structure:
(a) Ionic Bonding:

General characteristics of ionic compounds: ionization energy, electron affinity etc. Sizes of Ions,
radius ratio rule and its limitation. Lattice energy, Born-Haber cycle.
(b) Covalent Bonding:
General characteristics of covalent compounds, valence bond approach, directional character of

covalent bond, hybridization involving s-, p- and d- orbitals. Valence State Electron Pair Repulsion
(VSEPR) concept, shapes of simple molecules and ions. Fajan’s Rules, M.O. Theory. Hydrogen
bonding and its effect on physical and chemical properties. Others types of molecular interaction.
Acids-Bases and Solvents:

Modern concepts of acids and bases: Arrhenius theory, theory of solvent system, Bronsted and
Lowry’s concept, Lewis concept with typical examples, applications and limitations. Strengths of
acids and bases (elementary idea). Ionization of weak acids and bases in aqueous solution, ionization
constants, ionic product of water, pH scale
Nomenclature and Bonding in organic compounds :

Classification, trivial names and IUPAC system of nomenclature of organic compounds. Nature of
covalent bond and its orbital representation. Hybridization, bond energy, polarity of bond & dipole
moment of molecules, inductive effect, hydrogen bond, conjugation, resonance.
Haemolytic&heterolytic fission of bonds electrophiles & nucleophiles, carbocation, carbanions and
radicals- there stability, geometry & generation.
Alkanes, Alkenes, Alkynes:

Isomerism, synthesis , chemical reactivity of alkanes, Mechanism of free radical halogenation of
11
alkanes, sulphonation of alkanes. Chemical reactivity, hydrogenation, heat of hydrogenation and

stability of alkanes, electrophilic addition reaction & mechanism, halogenation, hydrohalogenation,
hydration, hydroboration, Markownikoffs rule, peroxide effect, 1-3 dipolar addition (only formation
no details mechanism is required). Alkyne synthesis hydration, substitution reactions, polymerization.
Mechanism of SN1 & SN2 reactionSNi reaction, E1&E2 reaction (elementary treatment) of aliphatic
hydrocarbon. Saytzeff& Hofmann elimination.
Aromatics Hydrocarbons and Aromatic substitution reactions :

Isomerism of aromatic compounds, their nomenclature, structure of benzene ring. General mechanism
of aromatic electrophilic substitution (elementary treatment)
Methods of synthesis, nitration, Sulphonation, halogenation.

Friedel-crafts alkylation and acylation, reaction, nuclear and side chain halogination. Mechanism of
Nucleophilic and electrophilic aromatic substitution.
Stereochemistry:
Dissymmetric Molecules: Different types of Isomerism,Structural Isomers, Geometrical,
Stereoisomerism, Configurational Isomers, Conformational Isomers, Concept of asymmetric carbon
atom, Enantiomers, Diastereiosmers, Stereogenic atom / center, Chirotopic / Achirotopic Centre,
Protereoisomerism, Concept of Topicity of Ligands and Faces (Homotopic, Enantiotopic,
Diastereotopic atoms and groups; Prochiral, Homotopic, Enantiotopic, Diastereotopic Faces),
Projection Structures of Streoisomers (Fischer, Sawhorse, Newman, Flying-Wedge projection and
Interconversion of these projections formulas) of simple molecules containing one or two asymmetric
carbon atom, Optical isomerism, Optical activity, Element of symmetry and chirality,Meso
compounds, Chiral centers and the number of stereoisomers, Racemic modifications,Racemic mixture
or (+/-)-Conglomerate, Racemic Compounds or racenate, Stereochemical nomenclature of
Stereoisomers containing chiral centers(R/S and E/Z or cis-trans or sec cis- sec trans of C=C
system);D,L system of designation; Pro-R, Pro-S, Re, Si, Erythro, threo, Pref and Praf designation of
enantiotopic groups and atoms; Chirality of Organic molecules without chiral center and concept of
chiral axis.
Alcohols, Ethers and phenols:

Methods of synthesis, physical properties, distinction of primary, secondary and tertiary alcohols.
Chemical reactivity. Ethers, methods of synthesis, Chemical reactivity. physical properties acidic
character of phenols, chemical reaction –Reimer-Tiemann reaction, Fries rearrangement, Kolbe’s
reaction, phenol formaldehyde resins (Lederer-Manasse reaction) Cresols, nitro and amino
phenols.(Synthesis only).
Aldehydes and ketones:

Methods of synthesis of aldehydes and ketones, chemical reactivity of carbonyl group, cannizzaro
reaction and aldol condensation, relative reactivities of aldehyde and ketones. Perking reaction,
benzoine condensation, Claisen condensation.
Carboxylic acid and their derivatives:

Methodes of synthesis, acidity of aliphatic and aromatic acid, effects of substitutents on acidity
(simple cases). Chemicalreactivity. Mechanism of esterification. Methods of synthesis and reaction
of acid halides, amides, esters and anhydrides.
BSUBTC-392: (Lab)[Lab on Chemistry] Credit 2

1. Qualitative organic analysis:

Detection of elements (N,S,Cl,Br,I ), unsaturation & all the functional groups ( alcoholic & phenolic
12
hydroxyl/ aldehydic & ketonic carbonyl / carboxylic acid & aromatic amino, anilide and nitro)
present in a supplied mono- or bi- functional organic compounds.
2. Gravimetric Analysis :
Techniques of Precipitations, filtration, washing, drying, igniting and weighing
precipitates.Gravimatric estimation of any ion. Determination of hardness water.
3. Qualitative Inorganic Analysis:

Estimation of glucose & phenol. sulphaides, sulphites, sulphates, nitrites, nitrates, nitrites, &
phosphates, (Acid insoluble compounds & phosphate separation omitted).
1. Inorganic Chemistry by R. L. Dutta
2. Organic Chemistry by I. L. Finer (Vol. I)
3. Advanced practical chemistry, 3rd edition by Subhas C Das
4. An advanced course in practical chemistry by Ghoshal, Mahapatra and Nad.
BSUBTC- 303: Molecular Biology Credit4
UNIT I
DNA structure and replication.
DNA as genetic material, structure of DNA, Types of DNA, Replication of DNA in prokaryotes and
Eukaryotes, Semiconservative nature of DNA replication, Bi-directional replication, DNA
polymerases, The replication complex: Pre-primming proteins, primosome, replisome, Rolling circle
replication, Unique aspects of eukaryotic chromosome replication, Fidelity of replication.
UNIT II
DNA damage, repair, nonhomologous and homologous recombination.
DNA damage and repair: causes and types of DNA damage, mechanism of DNA repair: Photo-
reactivation, base excision repair, nucleotide excision repair, mismatch repair, translesion synthesis,
recombinational repair, nonhomologous end joining. Homologous recombination:models and
mechanism.
UNIT III
Transcription and RNA processing.
RNA structure and types of RNA, Transcription in eukaryotes: Eukaryotic RNA polymerases,
transcription factors, promoters,enhancers, mechanism of transcription initiation, promoter clearance
and elongation RNA splicing and processing: processing of pre-mRNA: 5’ cap formation,
polyadenylation, splicing, rRNA and tRNA splicing.
UNIT IV
Regulation of gene expression and translation.
Regulation of gene expression in prokaryotes: Operon concept (inducible and system), Genetic code
and its characteristics, Prokaryotic and eukaryotic eukaryotic translation: ribosome structure and
assembly, Charging of tRNA, aminoacyl tRNA synthetases, Mechanism of initiation, elongation and
termination of polypeptides, Fidelity of translation, Inhibitors of translation., Posttranslational
modifications of proteins.
UNIT V
How to clone a gene.
What is clone, overview of the procedure, Gene library, hybridization Cutting and Joining DNA-
Restriction Endonucleases, Ligation, Alkaline phosphate, Modification of Restriction fragment ends,
Other ways of joining DNA molecules. Plasmid vectors, Vectors based on the lambda
bacteriophage,cosmids, M13 vectors, Expression vectors, Vectors for cloning and expression in
13
Eukaryotic cells, Super vectors- YACs and BACs
BSUBTC- 393(Lab) [lab On Molecular Biology] Credit2
1. Preparation of buffers and solutions for molecular biology experiments
2. DNA isolation from Cabbage leaves/ goat liver/Human blood and Microbes
3. Plasmid DNA isolation
4. Agarose gel Electrophoresis of genomic DNA and plasmid DNA
5. Preparation of restriction digestion of DNA samples
6. Gel Documentation and photography Learning Resources
Reference:
1. Gene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th
edition,Pearson Benjamin Cummings Publishing, San Francisco
3. De Robertis EDP and De Robertis EMF (2006) Cell and Molecular Biology, 8th edition.
Lippincott Williams and Wilkins, Philadelphia
4. Karp G (2010) Cell and Molecular Biology: Concepts and Experiments, 6th edition, John
Wiley & Sons. Inc.
5. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4th Edition,
Cold Spring Harbour Laboratory press.
6. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and
Bartlett Learning
7. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India.
BSUBTS- 301:Enzymology Credit2
UNIT - I
Isolation, and purification of enzymes, Enzyme classification (rationale, overview and specific
examples) Enzyme substrate complex: concept of E-S complex, binding sites, active site, specificity,
Kinetics of enzyme activity, Michaelis-Menten equation and its derivation, Different plots for the
determination of Km and Vmax and their physiological significance, factors affecting initial rate, E,
S, temp. &pH.
UNIT - II
Mechanism of enzyme action: General mechanistic principle, factors associated with catalytic
efficiency: proximity, orientation, distortion of strain, acid-base, nucleophilic and covalent catalysis.
Techniques for studying mechanisms of action, chemical modification of active site groups, specific
examples-: chymotrypsin, Iysozyme, GPDH, aldolase, RNase, Carboxypeptidase and alcohol
dehydrogenase. Enzyme regulation: Product inhibition, feed backcontrol, covalent modification.
UNIT – III
Allosteric enzymes with special reference to aspartate transcarbomylase and phosphofructokinase.
Qualitative description of concerted and sequential models. Negative cooperativity and half site
reactivity. Enzyme - Enzyme interaction, Protein ligand binding, measurements analysis of binding
isotherm, cooperativity, Hill and scatchard plots, kinetics of allosteric enzymes
UNIT - IV
Enzyme Technology: Methods for large scale production of enzymes. Immobilized enzyme and their
comparison with soluble enzymes, Methods for immobilization of enzymes. Immobilized enzyme
14
reactors. Application of Immobilized and soluble enzyme in health and industry. Application to
fundamental studies of biochemistry.
Learning Resources
1. Biochemistry, LubertStryer, 6th Edition, WH Freeman, 2006.
2. Harper’s illustrated Biochemistry by Robert K. Murray, David A Bender, Kathleen
M.Botham, Peter J. Kennelly, Victor W. Rodwell, P. Anthony Weil. 28th Edition, McGrawHill, 2009.
3. Biochemistry, Donald Voet and Judith Voet, 2nd Edition, Publisher: John Wiley and Sons,
1995.
4. Biochemistry by Mary K.Campbell& Shawn O.Farrell, 5th Edition, Cenage Learning,2005.
5. Fundamentals of Enzymology Nicholas Price and Lewis Stevens Oxford University Press
1999
6. Fundamentals of Enzyme Kinetics Athel Cornish-Bowden Portland Press 2004
7. Practical Enzymology Hans Bisswanger Wiley–VCH 2004
8. The Organic Chemistry of Enzyme-catalyzed Reactions Richard B. Silverman Academic
Press 2002.
BSUBTS-302 Industrial Biotechnology Credit2
UNIT I
Production of industrial chemicals, biochemicals and chemotherapeutic products. Propionic acid,
butyric acid, 2-3 butanediol, gluconic acid, itaconic acid, Biofuels: Biogas, Ethanol, butanol,
hydrogen, biodiesel, microbial electricity, starch conversion processes; Microbial polysaccharides;
Microbial insecticides; microbial flavours and fragrances, newer antibiotics, anti cancer agents, amino
acids.
UNIT II
Microbial products of pharmacological interest, steriod fermentations and transformations. Over
production of microbial metabolite, Secondary metabolism – its significance and products. Metabolic
engineering of secondary metabolism for highest productivity. Enzyme and cell immobilization
techniques in industrial processing, enzymes in organic synthesis, proteolytic enzymes, hydrolytic
enzymes, glucose isomerase, enzymes in food technology/organic synthesis.
UNIT III
Purification & characterization of proteins, Upstream and downstream processing, solids and liquid
handling. Distribution of microbial cells, centrifugation, filtration of fermentation broth, ultra
centrifugation, liquid extraction, ion-exchange recovery of biological products. Experimental model
for design of fermentation systems, Anaerobic fermentations.
UNIT IV
Rate equations for enzyme kinetics, simple and complex reactions. Inhibition kinetics; effect of pH
and temperature on rate of enzyme reactions. Mathematical derivation of growth kinetics,
mathematical derivations of batch and continuous culture operations; single stage CSTR; mass
transfer in aerobic fermentation; resistances encountered; overall mass transfer co-efficient (Ka)
determination, factors depending on scale up principle and different methods of scaling up. Metabolic
engineering of antibiotic biosynthetic pathways.
Learning Resource
1. Casida LE. (1991). Industrial Microbiology. 1st edition. Wiley Eastern Limited.
2. Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology.
2nd edition. Panima Publishing Co. New Delhi.
3. Patel AH. (1996). Industrial Microbiology. 1st edition, Macmillan India Limited.
4. Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd
edition, Elsevier Science Ltd.
5. Salisbury, Whitaker and Hall. Principles of fermentation Technology,
15
BSUBTS-303 Plant and Animal Chromosome Preparation and Karyotyping Credit-2
1. Basic Principle of Cytogenetics Procedure- Specimen procurement , culture

procedure,harvesting, slide making (plant and animal)
2. Chromosome Preparation from Different Plant Parts- basic procedures
3. Peripheral Blood and Bone Marrow Culture- sample collection , setting up of culture, media
preparation and culture procedure, significance of different types of culture
4. Chromosome Staining (Plant and Animal)- Acetoorcein and feulgen staining for plants –
principle and methods, conventional giemsa staining, differential staining techniques
5. Photomicrograph and Image Processing –basic concepts
6. Chromosome Analysis and Karyotype - Karyotyping of normal male and female individuals
and interpretentions, plant chromosomes grouping
7. Chromosome Identification- individual band position and characteristics
Learning resources-
Barch MJ et al. The AGT cytogenetics Laboratory Manual; 3rd ed,1007, Lippincott-Raven; New
York
PurandareHema&Chakravarty Amit: Human cytogenetics Techniques& clinical applications,2000,
Bhalani Publishing House, Mumbai
Culture of Animal cells-a manual of basic Techniques:R IAN Freshney (Wiley Publication)
Arun Kumar Sharma and Archana Sharma :2014 Chromosome Techniques Theory and Practice,
Butterworth-Heinemann, Oxford,
BSUBTG-3(1,2,3,….n): MOOCS Credit 6
MOOC Basket 1
MOOC Basket 2
MOOC Basket 3
MOOC Basket 4
16
SEMESTER-IV
BSUBTC-401: Immunology Credit 4
UNIT I
Introduction
Concept of Innate and Adaptive immunity; Contributions of following scientists to the development
of field of immunology - Edward Jenner, Karl Landsteiner, Robert Koch, Paul Ehrlich, Elie
Metchnikoff, Peter Medawar, MacFarlane Burnet, Neils K Jerne, Rodney Porter and Susumu
Tonegawa
UNIT II
Immune Cells and Organs
Structure, Functions and Properties of: Immune Cells – Stem cell, T cell, B cell, NK cell,
Macrophage, Neutrophil, Eosinophil, Basophil, Mast cell, Dendritic cell; and Immune Organs – Bone
Marrow, Thymus, Lymph Node, Spleen, GALT, MALT, CALT
UNIT III
Antigens
Characteristics of an antigen (Foreignness, Molecular size and Heterogeneity); Haptens; Epitopes (T
& B cell epitopes); T-dependent and T-independent antigens; Adjuvants
UNIT IV
Antibodies
Structure, Types, Functions and Properties of antibodies; Antigenic determinants on antibodies
(Isotypic, allotypic, idiotypic); Monoclonal and Chimeric antibodies
UNIT V
Major Histocompatibility Complex
Organization of MHC locus (Mice & Human); Structure and Functions of MHC I & II molecules;
Antigen processing and presentation (Cytosolic and Endocytic pathways)
UNIT VI
Complement System
Components of the Complement system; Activation pathways (Classical, Alternative and Lectin
pathways); Biological consequences of complement Activation
UNIT VII
Generation of Immune Response
Primary and Secondary Immune Response; Generation of Humoral Immune Response (Plasma and
Memory cells); Generation of Cell Mediated Immune Response (Self MHC restriction, T cell
activation, Co- stimulatory signals); Killing Mechanisms by CTL and NK cells, Introduction to
tolerance
UNIT VIII
Immunological Disorders and Tumor Immunity
Types of Autoimmunity and Hypersensitivity with examples; Immunodeficiencies - Animal models
(Nude and SCID mice), SCID, DiGeorge syndrome, Chediak- Higashi syndrome, Leukocyte adhesion
deficiency, CGD; Types of tumors, tumor Antigens and cancer.
UNIT IX
Immunological Techniques
17
Principles of Precipitation, Agglutination, Immunodiffusion, Immunoelectrophoresis, ELISA,

ELISPOT, Western blotting, Immunofluoresence, Flow cytometry, Immunoelectron microscopy.
UNIT X
Vaccines & Vaccination
adjuvants, cytokines, DNA vaccines, recombinant vaccines, bacterial vaccines, viral vaccines,
vaccines to other infectious agents, tumor vaccines, principles of vaccination, passive & active
immunization, immunization programs & role of WHO in immunization programs.
BSUBTC- 491(Lab)[ Immunology] Credit 2
1. Identification of human blood groups.

2. Perform Total Leukocyte Count of the given blood sample.
3. Perform Differential Leukocyte Count of the given blood sample.
4. Separate serum from the blood sample (demonstration).
5. Perform immunodiffusion by Ouchterlony method.
6. Immunoelectrophoresis
7. Antigen- antibody reaction (Coomb’s test)
8. ELISA.
9. antibody and antigen(Ouchterlony method)
Learning Resources
1. Abbas AK, Lichtman AH, Pillai S. (2007). Cellular and Molecular Immunology. 6 th edition
Saunders Publication, Philadelphia.
2. Delves P, Martin S, Burton D, Roitt IM. (2006). Roitt’s Essential Immunology. 11th edition
Wiley-Blackwell Scientific Publication, Oxford.
3. Goldsby RA, Kindt TJ, Osborne BA. (2007). Kuby’s Immunology. 6th edition W.H. Freeman
and Company, New York.
4. Murphy K, Travers P, Walport M. (2008). Janeway’s Immunobiology. 7th edition Garland
Science Publishers, New York.
5. Peakman M, and Vergani D. (2009). Basic and Clinical Immunology. 2nd edition Churchill
Livingstone Publishers, Edinberg.
6. Richard C and Geiffrey S. (2009). Immunology. 6th edition. Wiley Blackwell Publication.
BSUBTC-402: Chemistry II Credit 4
Chemical analysis :
i) Comparative study of the following groups of elements:
(a) B, Al (b) C, Si, Ge, Sn, Pb (c) N, P, As, Sb, Bi, (d) O, S, Se, Te (e) F,Cl, Br,I
In respect of electronic configuration, elemental states, oxidation states, hydrides, halides, oxides, and
oxyacides.
Double & complex salt:

Werner’s theory of co-ordination compounds. Chelates. Polydentate ligands including naturally
occurring ones. Electronic interpretation of compounds formation. Stepwise and overall stability
constants. (elementary idea only) Geometrical & optical isomerism. Nomenclature of coordination
compounds.
Interhalogen compounds:
18
Basic properties of iodine, pseudo halogens.
Organometallic Compounds:
Organomagnesium Compounds, Organozinc Compounds, Organolead Compounds, Organocadmium
Compounds.
Bio-inorganic chemistry
Role of metal complexes in biological system : Role of Iron and Magnesium
Ideal and real Gases:

Distribution of molecular velocities, root-mean-square velocity, kinetic molecular theory of ideal
gases, deduction of kinetic gas equation. P=⅓mnc2, deduction of gases laws. Deviations of real gas
from ideal behavior, vanderwaal’s equation. Andrews experiment, critical phenomena in light of
vanderwaal’s equation of state, law of corresponding state.
Thermodynamics and Homogeneous chemical equilibrium:

Cyclic process, Reversible & irreversible process , internal energy, enthalpy, work Done, an
isothermal & adiabatic process, heat capacities, Cp-Cv =R for an ideal gas. Thermochemistry,
Carnot cycle, Elementary treatment of entropy, free entry, work function & criterion of equilibrium.
Gibbs Helmohltz equation, ClasiousClapeyron equation and its application. Law of mass action and
equilibrium constant Kp,Kc,Kx and their relationship. Le-chatelier’s principal- effect of temperature,
pressure and addition of products and inert gases. vant’shoff equation (derivation not required) and its
application.
Solubility and Ionic Equilibrium:

Solubility product, common ion effect and factors of solubility. Strong and weak electrolytes degree
of dissociation. Ostwald’s dilution law. Hydrolysis, buffer, calculation of pH, salt effect, elementary
idea of activity & activity co-efficient of electrolytes, ionic strength, buffer reaction of blood.
EMF :
Electrochemical cells, half-cell, electrode potential, standard electrode potential, Nernst equation,
redox potential, reference electrode,standered cell, measurement of emf, determination of pH,
potentiometric titration, storage battery, corrosion.
Dilute solution:
Rault’s law, ideal solution, non- ideal solution, and qualitative treatment of colligative properties
relative lowering of vapour pressure, elevation of boiling point, and osmotic pressure-their application
in finding molecular weight. Van’t Hoff ‘i’ factor, plasmolysis, haemolysis, isotonic solution, normal
saline, role of osmosis in living organism.
Reference books:
1. Inorganic Chemistry by R. L. Dutta
2. Organic Chemistry by I. L. Finer (vol. I)
3. Physical chemistry by P. C. Rakshit
BSUBTC-492: (Lab)[lab On Chemistry] Credit-2

1. Quantitative inorganic analysis

2. Preparation and standardization Mohr’s solution by KMnO4 solution.
3. Preparation of standard K2Cr2O7 solution and standardization
4. Mohr’s Salt solution.
5. Sodium thiosulphate solution.
6. Estimation of Fe(II) +Fe (III) mixture using standard solution of K2Cr2O7
7. Determination of Cu (II) using standard sodium thiosulphatesolution.
19
BSUBTC-403 Bio-Analytical Tools Credit 4
UNIT I
Simple microscopy, phase contrast microscopy, fluorescence and electron microscopy (TEM and
SEM), pH meter
UNIT II
Absorption Spectroscopy – Simple theory of the absorption of light by molecules, Beer-Lambert law,
Instrumentation for measuring the absorbance of visible light, Factors affecting the absorption
properties of a Chromophore. Principle of absorption fluorimetry,
UNIT III
Centrifugation – Basic Principle of Centrifugation, Instrumentation of Ultracentrifuge (Preparative,
Analytical), Factors affecting Sedimentation, Standard Sedimentation Coefficient, Rate-Zonal
centrifugation, sedimentation equilibrium Centrifugation. Cell fractionation techniques, isolation of
sub- cellular organelles and particles.
UNIT IV
Introduction to the principle of chromatography. Paper chromatography, thin layer chromatography,
column chromatography: silica and gel filtration, affinity and ion exchange
chromatography, gas chromatography, HPLC. Introduction to electrophoresis. Starch-gel,
polyacrylamide gel (native and SDS-PAGE), agarose-gel electrophoresis, pulse field gel
electrophoresis, immunoelectrophoresis, isoelectric focusing, Western blotting.
UNIT V
Mass spectrometry( MALDI, ESI) and Introduction to Biosensors and Nanotechnology and their
applications. Radioactive labeling & counting, Autoradiography.
UNIT VI
X-Ray Crystallography – X-ray diffraction, Bragg equation, Reciprocal lattice, Miller indices & Unit
cell, Concept of different crystal structure, determination of crystal structure [concept of rotating
crystal method, powder method].
UNIT VII
NMR Spectroscopy – Basic principle of NMR spectroscopy, Experimental technique &
instrumentation, Chemical shift, hyperfine splitting, Relaxation process
BSUBTC-492 (Lab)[Bio-Analytical Tools] Credit 2
1. Microscopy-light microscopy: principles, , parts and function, operation

2. Principles and operations of incubators, centrifuge
3. Principles and operations of pH meter and colorimeter
4. Determination pH of unknown solution
5. Native gel electrophoresis of proteins
1. Separation of sample mixture by column chromatography
2. Principles and operations of spectrophotometer
3. To identify lipids in a given sample by TLC.
4. Separation of amino acids by paper chromatography
5. Preparation of the sub-cellular fractions of liver cells.
20
Learning Resources:
1. Karp, G. 2010. Cell and Molecular Biology: Concepts and Experiments. 6th Edition. John
Wiley& Sons. Inc.
2. De Robertis, E.D.P. and De Robertis, E.M.F. 2006. Cell and Molecular Biology. 8th edition.
Lippincott Williams and Wilkins, Philadelphia.
3. Cooper, G.M. and Hausman, R.E. 2009. The Cell: A Molecular Approach. 5th edition. ASM,
Press & Sunderland, Washington, D.C.; Sinauer Associates, MA.
4. Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. 2009
5. The World of the Cell.7th edition. Pearson Benjamin Cummings Publishing, San Francisco.
BSUBTS-401 : Molecular Diagnostics Credit-2
UNIT I
Enzyme Immunoassays: Comparison of enzymes available for enzyme immunoassays, conjugation of
enzymes. Solid phases used in enzyme immunoassays. Homogeneous and heterogeneous enzyme
immunoassays. Enzyme immunoassays after immuno blotting. Enzyme immunohistochemical
techniques. Use of polyclonal or monoclonal antibodies in enzymes immuno assays. Applications of
enzyme immunoassays in diagnostic microbiology
UNIT II
Molecular methods in clinical microbiology: Applications of PCR, RFLP, Nuclear hybridization
methods, Single nucleotide polymorphism and plasmid finger printing in clinical microbiology
Laboratory tests in chemotherapy: Susceptibility tests: Micro-dilution and macro-dilution broth
procedures. Susceptibility tests: Diffusion test procedures. Susceptibility tests: Tests for bactericidal
activity. Automated procedures for antimicrobial susceptibility tests (.Lab – Demonstration of RAPD,
Kirby-Bauyer method (disc-diffusion method) to study antibiotic sensitivity of a bacterial culture)
UNIT III
Automation in microbial diagnosis, rapid diagnostic approach including technical purification and
standardization of antigen and specific antibodies. Concepts and methods in idiotypes. Antiidiotypes
and molecular mimicry and receptors. Epitope design and applications. Immunodiagnostic tests.
Immuno florescence. Radioimmunoassay.
UNIT IV
GLC, HPLC, Electron microscopy, flowcytometry and cell sorting.
Reference:
Culture of Animal cells-a manual of basic Techniques:R IAN Freshney (Wiley Publication)
Arun Kumar Sharma and Archana Sharma :2014 Chromosome Techniques Theory and Practice,
Butterworth-Heinemann, Oxford,
BSUBTS- 402 : Plant-Microbe Interaction Credit-2
UNITI:General account about the microbes used as biofertilizer – Rhizobium – isolation,

identification, mass multiplication, carrier based inoculants, Actinorrhizal symbiosis.
UNIT II:Azospirillum: isolation and mass multiplication – carrier based inoculant, associative effect
of different microorganisms. Azotobacter: classification, characteristics – crop response to
Azotobacter inoculum, maintenance and mass multiplication.
21
UNITIII: Cyanobacteria (blue green algae), Azolla and Anabaena azollae association, nitrogen
fixation, factors affecting growth, blue green algae and Azolla in rice cultivation.
UNITIV: Mycorrhizal association, types of mycorrhizal association, taxonomy, occurrence and

distribution, phosphorus nutrition, growth and yield – colonization of VAM – isolation and inoculum
production of VAM, and its influence on growth and yield of crop plants.
UNIT V: Organic farming – Green manuring and organic fertilizers, Recycling of biodegradable
municipal, agricultural and Industrial wastes – biocompost making methods, types and method of
vermicomposting – field Application.
Learning Resources
1. Dubey, R.C., 2005 A Text book of Biotechnology S.Chand& Co, New Delhi.
2. Kumaresan, V. 2005, Biotechnology, Saras Publications, New Delhi.
3. John Jothi Prakash, E. 2004. Outlines of Plant Biotechnology. Emkay Publication, New
Delhi.
4. Sathe, T.V. 2004 Vermiculture and Organic Farming. Daya publishers.
5. SubhaRao, N.S. 2000, Soil Microbiology, Oxford & IBH Publishers, New Delhi.
6. Vayas,S.C, Vayas, S. and Modi, H.A. 1998 Bio-fertilizers and organic Farming
AktaPrakashan, Nadiad
BSUBTS-403 Research Methodology Credit-2
UNITI: Foundations of Research

Meaning, Objectives, Motivation: Research Methods vs Methodology, Types of Research: Analytical
vs Descriptive, Quantitative vs Qualitative, Basic vs Applied
UNIT II: Research Design

Need for research design: Features of good design, Important concepts related to good design-
Observation and Facts, Prediction and Explanation, Development of Models. Developing a research
plan: Problem identification, Experimentation, Determining experimental and sample designs
UNIT III: Data Collection, Analysis and Report Writing

Observation and Collection of Data-Methods of data collection- Sampling Methods, Data Processing
and Analysis Strategies, Technical Reports and Thesis writing, Preparation of Tables
andBibliography. Data Presentation using digital technology.
UNITIV: Ethical Issues

Concepts of Copy Right, Royalty, Patent law, Plagiarism, Citation, Acknowledgement
BSUBTS-404 Basics of Forensic Science Credit-2
UNIT I
Introduction and principles of forensic science, forensic science laboratory and its organization and
service, tools and techniques in forensic science, branches of forensic science, causes of crime, role of
modus operandi in criminal investigation. Classification of injuries and their medico-legal aspects,
method of assessing various types of deaths.
UNIT II
Classification of fire arms and explosives, introduction to internal, external and terminal ballistics.
Chemical evidence for explosives. General and individual characteristics of handwriting,
examination and comparison of handwritings and analysis of ink (various samples).
22
UNIT III
Role of the toxicologist, significance of toxicological findings, Fundamental principles
offingerprinting, classification of fingerprints, development of finger print as science for personal
identification.
UNIT IV
Principle of DNA fingerprinting, application of DNA profiling in forensic medicine, Investigation
Tools, e-Discovery, Evidence Preservation, Search and Seizure of Computers, Introduction to Cyber
security.(Lab- Demo on PCR amplification on target DNA and DNA profiling).
BSUBTG-4(1,2,3,….n): MOOCS Credit 6

MOOC Basket 1
MOOC Basket 2
MOOC Basket 3
MOOC Basket 4
23
SEMESTER-V
BSUBTC-501:Bioprocess Technology Credit 4
UNIT I
Introduction to bioprocess technology. Range of bioprocess technology and its chronological
development. Basic principle components of fermentation technology. Types of microbial culture and
its growth kinetics– Batch, Fedbatch and Continuous culture.
UNIT II
Design of bioprocess vessels- Significance of Impeller, Baffles, Sparger; Types of culture/production
vessels- Airlift; Cyclone Column; Packed Tower and their application in production processes.
Principles of upstream processing – Media preparation, Inocula development and sterilization.
UNIT III
Introduction to oxygen requirement in bioprocess; mass transfer coefficient; factors affectingKLa.
Bioprocess measurement and control system with special reference to computer aided process control.
UNIT IV
Introduction to downstream processing, product recovery and purification. Effluent treatment.
Microbial production of ethanol, amylase, lactic acid and Single Cell Proteins.
BSUBTC- 591Bioprocess technology (Lab) Credit 2
1. Bacterial growth curve.

2. Calculation of thermal death point (TDP) of a microbial sample.
3. Production and analysis of ethanol.
4. Production and analysis of amylase.
5. Production and analysis of lactic acid.
Suggested Reading
1. Shuler, M. L., & Kargi, F. (2002). Bioprocess Engineering: Basic Concepts. Upper Saddle
River, NJ: Prentice Hall
Casida LE. (1991). Industrial Microbiology. 1st edition. Wiley Eastern Limited.
2. Crueger W and Crueger A. (2000). Biotechnology: A textbook of Industrial Microbiology.
2nd edition. Panima Publishing Co. New Delhi.
3. Patel AH. (1996). Industrial Microbiology. 1st edition, Macmillan India Limited.
4. Stanbury PF, Whitaker A and Hall SJ. (2006). Principles of Fermentation Technology. 2nd
edition, Elsevier Science Ltd.
BSUBTC-502 Recombinant DNA Technology Credit 4
UNIT I
Molecular tools and applications- restriction enzymes, ligases, polymerases, alkalinephosphatase.
Gene Recombination and Gene transfer: Transformation, Episomes, Plasmids and other cloning
vectors (Bacteriophage-derived vectors, artificial chromosomes), Microinjection,Electroporation,
24
Ultrasonication, Principle and applications of Polymerase chain reaction (PCR),primer-design, and

RT- (Reverse transcription) PCR.
UNIT II
Restriction and modification system, restriction mapping. Southern and Northern
hybridization.Preparation and comparison of Genomic and cDNA library, screening of recombinants,
reverse transcription,. Genome mapping, DNA fingerprinting, Applications of Genetic
Engineering,Genetic engineering in animals: Production and applications of transgenic mice, role of
ES cells in gene targeting in mice, Therapeutic products produced by genetic engineering-blood
proteins, human hormones, immune modulators and vaccines (one example each).
UNIT III
Random and site-directed mutagenesis: Primer extension and PCR based methods of site directed
mutagenesis, Random mutagenesis, Gene shuffling, production of chimeric proteins, Protein
engineering concepts and examples (any two).
UNIT IV
Genetic engineering in plants: Use of Agrobacterium tumefaciens and A. rhizogenes, Ti plasmids,
Strategies for gene transfer to plant cells, Direct DNA transfer to plants, Gene targeting in plants, Use
of plant viruses as episomal expression vectors.
BSUBTC-592: Recombinant DNA Technology (Lab) Credit 2
1. Isolation of chromosomal DNA from plant cells

2. Isolation of chromosomal DNA from E.coli
3. Qualitative and quantitative analysis of DNA using spectrophotometer
4. Plasmid DNA isolation
5. Restriction digestion of DNA
6. Making competent cells
7. Transformation of competent cells.
8. Demonstration of PCR
Learning resources:
1. Brown TA. (2006). Gene Cloning and DNA Analysis. 5th edition. Blackwell Publishing,
Oxford, U.K.
2. Clark DP and Pazdernik NJ. (2009). Biotechnology-Applying the Genetic Revolution.
Elsevier Academic Press, USA.
3. Glick, B.R., Pasternak, J.J. (2003). Molecular Biotechnology- Principles and Applications of
recombinant DNA. ASM Press, Washington
4. Primrose SB and Twyman RM. (2006). Principles of Gene Manipulation and Genomics, 7th
edition. Blackwell Publishing, Oxford, U.K.
5. Sambrook J, Fritsch EF and Maniatis T. (2001). Molecular Cloning-A Laboratory Manual.
3rd edition. Cold Spring Harbor Laboratory Press.
BSUBTD- 501A Animal Biotechnology Credit: 6
1. Gene transfer methods in Animals – Microinjection, Embryonic Stem cell gene transfer,
Retrovirus & Gene transfer.
2. Transgenic Animals – Mice, Cow, Pig, Sheep, Goat, Bird, Insect.
25
3. Animal diseases need help of Biotechnology – Foot-and-mouth disease, Coccidiosis,

Trypanosomiasis, Theileriosis.
4. Animal propagation – Artificial insemination, Animal Clones.
5. Cryopreservation technology Basic techniques,–Animal cell culture and cryopreservation,
Embryo conservation techniques Role of cryopreservation in assisted reproductive technology.
SUGGESTED READING
1. Brown, T.A. (1998). Molecular biology Labfax II: Gene analysis. II Edition. Academic Press,
California,USA.
2. Butler, M. (2004). Animal cell culture and technology: The basics. II Edition. Bios scientific
publishers.
3. Glick, B.R. and Pasternak, J.J. (2009). Molecular biotechnology- Principles and applications
of recombinant DNA. IV Edition. ASM press, Washington, USA.
4. Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An
introduction to genetic analysis. IX Edition. Freeman & Co., N.Y., USA.
5. Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA genes
and genomes- A short course. III Edition. Freeman and Co., N.Y., USA.
BSUBTD- 502 A: Model Organism and Human Genome Project Credit: 6
UNIT I
Genome – about genomes of model organisms (E. coli, Yeast, Arabidopsis thaliana, C. elegans,
Drosophila melanogaster, laboratory mouse, Zebra fish, Human), types of genomes, genomes &
genetic variation, comparison of different genomes, genome evolution.
Genomics – about the genomics, history, comparative genomics, comparative genomic hybridization,
functional genomics.
UNIT II
Genome projects – an overview of genome projects of human and other model organisms of Human
Genome Project.
Human Genome Project (HGP) – an overview of the project, goals of the project, major scientific
strategies & approaches used in HGP, expected scientific & medical benefits of this project, about the
organizations behind this project.
UNIT III
How Human genome was mapped – physical mapping, genetic mapping, gene ontology,
geneannotation.
UNIT IV
Technologies used in HGP – RFLP, microsatellite markers, STS, EST, DNA sequencing, DNA
microarray.
BSUBTD- 503A Medical Biotechnology Credit: 6
UNIT I: Gene therapy – background, types of gene therapy (ex vivo & in vivo), choosing targets for
gene therapy, vectors in gene therapy, retroviruses, adenoviruses, adeno-associated viruses, types of
gene delivery, Weismann barrier (soma-to-germ line barrier), epigenetic inheritance, problems &
ethics.
Gene Delivery methods – Viral delivery (through Retroviral vectors, through Adenoviral vectors),
Non- viral delivery, Antibody engineering.
Gene therapy Models – Liver diseases, Lung diseases, Hematopoietic diseases, Circulated gene
products, Cancer & Auto-immune diseases.
26
UNIT II: Vaccines – Vaccine vectors, nucleic acid vaccines, immuno-enhancing technology.
Synthetic therapy – synthetic DNAs, therapeutic Ribozymes, synthetic drugs.
UNIT III: Tissue Engineering – Skin, Liver, Pancreas.

Xenotransplantation – terminology, technology behind it, organ donors, social &ethical issues.
Cell Adhesion-based therapy – integrins, inflammation, cancer & metastasis.
UNIT IV: Drug delivery – conventional & new approaches to drug delivery.
BSUBTD- 501B : Plant Biotechnology Credit: 6
UNIT I
Plant Tissue Culture applications – Introduction, organogenic differentiation, Types of culture: Seed ,
Embryo, Callus, Organs, Cell. Micropopagation of Axillary bud proliferation, Meristem and shoot tip
culture, organogenesis, embryogenesis, advantages and disadvantages of micropropagation.
UNIT II
In vitro haploid production Androgenic methods: Anther culture, Microspore culture Andogenesis,
Double haploid production Significance and use of double haploids, Gynogenic haploids, factors
effecting gynogenesis.
UNIT III
Protoplast Isolation and fusion Methods of protoplast isolation, Protoplast development, Somatic
hybridization, identification and selection of hybrid cells, Cybrids, Potential of somatic hybridization
limitations. Somaclonal variation.
UNIT IV
Applications of Plant Genetic Engineering – crop improvement, herbicide resistance,insect resistance,
virus resistance, plants as bioreactors.
Genetic modification in Agriculture – transgenic plants, genetically modified foods,application, future
applications, ecological impact of transgenic plants.
BSUBTD-502B : Plant Secondary Metabolites and Bio-transformation Credit-6
1. Introduction to primary & secondary metabolites: structure

2. Types of secondaryb metabolites -Glycosides, isoprenoids, cardenolides, alkaloids, and
phenylpropanoids
3. various Biotechnological Method for the Production of Secondary
4. biosynthesis of important secondary products-Alkaloids, Flavonoids
5. Important groups of secondary metabolites-Sources and uses
6. Importance of secondary metabolites
7. Production of secondary metabolites by bioconversion genetic transformation for production
of secondary metabolite
8. Basic concepts of Biotransformation. –Introduction, Applications and limitations.
27
SEMESTER-VI
BSUBTC-601: Genomics, Proteomics and Bioinformatics Credit 4
UNIT I: Introduction to Genomics:

Information flow in biology, DNA sequencing methods– manual & automated: Maxam & Gilbert and
Sangers method. Pyrosequencing, Genome Sequencing: Shotgun & Hierarchical (clone contig)
methods, Computer tools for sequencing projects: Genome sequence assembly software.
UNIT II: Managing and Distributing Genome Data:

Web based servers and softwares for genome analysis: ENSEMBL, VISTA, UCSC Genome
Browser, NCBI genome, GenBank, EMBL. Concept of INSDC, Selected Model Organisms' Genomes
and Databases.
UNIT III: Single Nucleotide Polymorphisms:

Genome variation; Single nucleotide polymorphism idea of Missense,Synonymous, FrameshiftSNPs,
SNP profiling, Disease and SNPs. Basic idea of DNA microarray and SNP array.
UNIT IV: Structure and properties of proteins

Introduction to protein structure, Chemical properties of proteins, Physical interactions that determine
the property of proteins. Determination of sizes (Sedimentation analysis, gel filtration, Native PAGE,
SDS- PAGE); Determination of covalent structures of proteins.
UNIT V: Introduction to Proteomics

Fundamental goals of proteomics, Analysis of proteomes. 2D-PAGE
(Samplepreparation,solubilization, reduction, resolution. Reproducibility of 2D-PAGE). Mass
spectrometry based methods for protein identification. De novo sequencing using mass spectrometric
data.
UNIT VI: Protein databases and networks:

protein sequence and structural data, protein information resources and secondary data bases, protein
data bank. Introduction to preliminary analysis of the transcriptome, Proteomics-Expression analysis
& Characterization of proteins, Protein microarray, Metabolomics & global biochemical networks.
UNIT VII: Introduction to Bioinformatics

History of Bioinformatics. Importance of Bioinformatics in the field of biology and healthcare, Goal
and Scope of bioinformatics. Central Dogma and bioinformatics.
UNIT VIII: Data Generation and Data Retrieval

Sequence submission tools (BankIt, Sequin); Sequence filenformat (flat file, FASTA, Genbank,
Genpept, EMBL, Swiss-Prot); D ata retrieval systems (NCBI Entrez).
UNIT IX: Sequence Alignment and Pattern recognition

Sequence similarity searching; Methods of Alignment (Dot matrix,Dynamic Programming, BLAST
and FASTA algorithm); Local and global alignment, pairwise and multiple sequence alignments
(without algorithm); Concept of identity and homology of sequences. Scoring Matrices (PAM,
BLOSUM).
Reference Books:
1. Ghosh Z. and Bibekanand M. (2008) Bioinformatics: Principles and Applications. Oxford
University Press.
2. Pevsner J. (2009) Bioinformatics and Functional Genomics. II Edition.Wiley-Blackwell.
3. Campbell A. M., Heyer L. J. (2006) Discovering Genomics, Proteomics and Bioinformatics.
28
II Edition. Benjamin Cummings.

4. David W Mount Bioinformatics: Sequence and Genome analysis Cold Spring Harbor
Laboratory Press.
5. Fundamentals of Biochemistry by Voet, Voet and Pratt.
BSUBTC-691: (Lab)[Lab on Genomics, Proteomics and Bioinformatics] Credit 2
1. Internet basics in hand (Introduction to computer hardware and software, Concept of intranet
and internet. LAN, MAN and WAN, IP address, MAC address. Internet Browsers and search engine.)
2. Introduction to NCBI
Database Handling of NCBI; PubMed, Nucleotide, Protein, Gene, SNP, EST, OMIM.Tools of
NCBI;Genome Browser, performing various kinds of blast.
3. Multiple Sequence alignment tool; Clustal W2
4. USING PIR,
5. Handling Structural data; PDB
6. Visualization of structures; using Rasmol.
BSUBTC-602 : IPR, Biosafety and Ethical Issues Credit 4
Introduction to Intellectual property Rights- Concept of IPR, different forms of IPR

Classification of patents, Special patents, Patenting biological products, Patentale and non patentable
inventions in India, grant of patents, Grant process and requirements,
Introduction and Overview of Biosafety, Categories and Cartagena protocol .Good laboratory
biosafety practices
Genetic technologies – an overview of Genetic screening for any predisposition symptoms, Cancer
screening, Cloning, Gene therapy, DNA fingerprinting,(Paternity and Forensics) in vitro fertilization,
surrogate motherhood, PGD, transgenic organisms, xenotransplantation, GMOs.
Ethical issues – ethical issues against the molecular technologies.
Bioethics – Necessity of Bioethics, Scope of bioethics, different paradigms of Bioethics – National &
International.
BSUBTC-692 [Bio-safety and ethical issues] (Lab) Credit 2
1. Proxy filing of Indian Product/ Process patent
2. Seminar presentation on Bio-safety
3. Seminar presentation on Bio-ethics
4. Assignments
BSUBTD-601A Genetic Modification In agriculture, food & medicine Credit 6
1. Genetic Modification – terminology, methods of genetic modification, Basics of genetic

modification of bacteria, plant & animal, controversies over genetic modification, policy around the
world (USA, European Union, EU regulation, Japan, China & other developing countries).
2. Genetic Modification in Agriculture – types of transgenic plants, genetically modified foods,
application, future applications, ecological impact of transgenic plants.
3. Genetically Modified Foods – organic foods, types of organic foods, identifying organic
foods, organic food & preservatives.
29
4. Genetic Modification in Food Industry – background, history, controversies over Risks

Examples of modification and future application.
5. Genetic Modification in Medicine – gene therapy, types of gene therapy, vectors in gene
therapy, nanotechnology in gene therapy, molecular engineering, human genetic engineering,
problems & ethics.
BSUBTD-602A Environmental Biotechnology Credit 6
UNIT I
Conventional fuels and their environmental impact – Firewood, Plant, Animal, Water, Coal and Gas.
Modern fuels and their environmental impact – Methanogenic bacteria, Biogas, Microbial hydrogen
Production, Conversion of sugar to alcohol Gasohol
UNIT II
Bioremediation of soil & water contaminated with oil spills, heavy metals and detergents.
Degradation of lignin and cellulose using microbes. Phyto-remediation. Degradation of pesticides and
other toxic chemicals by micro-organisms- degradation aromatic and chlorinates hydrocarbons and
petroleum products.
UNIT III
Treatment of municipal waste and Industrial effluents. Bio-fertilizers, Role of symbiotic and
asymbiotic nitrogen fixing bacteria in the enrichment of soil. Algal and fungal biofertilizers (VAM)
UNIT IV
Bioleaching, Enrichment of ores by microorganisms (Gold, Copper and Uranium). Environmental
significance of genetically modified microbes, plants and animals.
1. Environmental Science, S.C. Santra
2. Environmental Biotechnology, Pradipta Kumar Mohapatra
3. Environmental Biotechnology – Concepts and Applications, Hans-Joachim Jordening and
Jesef Winter
4. Waste Water Engineering, Metcalf and Eddy, Tata McGraw hill
5. Agricultural Biotechnology, S.S. Purohit
6. Environmental Microbiology : Methods and Protocols, Alicia L. Ragout De Spencer, John
F.T. Spencer
7. Introduction to Environmental Biotechnology, Milton Wainwright
8. Principles of Environmental Engineering, Gilbert Masters
9. Wastewater Engineering – Metcalf & Eddy
BSUBTD- 681B : Dissertation on Biotechnology Credit6
A project work should be done individually under the guidance of one faculty member on any topic
related to the subject & can be recorded as dissertation & also be presented by the candidate in front
of externals in a seminar.
30

B.SC Biotechnology (Hons) In-House CBCS - 2020

Uploaded by

Document Informationclick to expand document information

Copyright:

Available Formats

B.SC Biotechnology (Hons) In-House CBCS - 2020

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

B.SC Biotechnology (Hons) In-House CBCS - 2020

Uploaded by

Copyright:

Available Formats

MAKAUT In-House B.Sc Biotechnology Hons.

Syllabus Structure [CBCS] 2020

CBCS – MAKAUT B.Sc Biotechnology B.Sc (Hons) 140 Credit FRAMEWORK

Total Credit- 12+2+6=20

Total Credit- 12+2+6=20

CORE COURSE ABILITY SKILL ENHANCEMENT GENERIC ELECTIVE DISCIPLINE SPECIFIC

Total Credit- 18+2+6=26

Total Credit- 12+12=24

Total Credit- 12+12=24

Credits point distribution per Semester:

MOOC Basket 1 MOOC Basket 2 MOOC Basket 3 MOOC Basket 4

HUMANITIES & HUMAN CREATIVE & GENERAL SCIENCE EMERGING TECH,

BSUBTC-191 (Lab) [Lab on Biochemistry and Metabolism]Credit 2

BSUBTC-102 Cell Biology Credit 4

BSUBTC-192 (Lab) )[Lab on Cell Biology] Credit 2

1. Preparation of Mitotic Chromosome from onion root tip.

Freeman and Company, New York, USA.

BSUBTA-101 English Communication Skill development Credit2

BSUBTG-1(1,2,3,….n): MOOCS Credit: 6

BSUBTC-201 General Microbiology Credit 4

BSUBTC-291 (Lab)[Lab on General Microbiology] Credit 2

thedition.ohn and Sons, Inc.

BSUBTC-202 Plant and Mammalian Physiology Credit 4

Excretion: modes of excretion, Ornithine cycle, Mechanism of urine formation

BSUBTC-292 (Lab)[Lab on Plant and mammalian Physiology] Credit 2

1. Finding the coagulation time of blood

BSUBTA-201: Introduction to Environmental Science Credit2

1. Carson, R. 2002. Silent Spring. Houghton Mifflin Harcourt.

BSUBTA-202 Introduction to Fundamental Computer Credit-2

BSUBTG-2(1,2,3,….n): MOOCS Credit: 6

BSUBTC- 301 Genetics Credit 4

BSUBTC-391: (Lab) [Lab on Genetics] Credit 2

4. Karyotyping with the help of photographs.

BSUBTC-302 Chemistry I Credit 4

Atomic Structure, radioactivity and Nuclear Structure of Atoms:

Chemical Bonding and Structure:

(a) Ionic Bonding:

General characteristics of covalent compounds, valence bond approach, directional character of

Acids-Bases and Solvents:

Nomenclature and Bonding in organic compounds :

Alkanes, Alkenes, Alkynes:

alkanes, sulphonation of alkanes. Chemical reactivity, hydrogenation, heat of hydrogenation and

Aromatics Hydrocarbons and Aromatic substitution reactions :

Methods of synthesis, nitration, Sulphonation, halogenation.

Alcohols, Ethers and phenols:

Aldehydes and ketones:

Carboxylic acid and their derivatives:

BSUBTC-392: (Lab)[Lab on Chemistry] Credit 2

1. Qualitative organic analysis:

3. Qualitative Inorganic Analysis:

BSUBTC- 303: Molecular Biology Credit4

Eukaryotic cells, Super vectors- YACs and BACs

BSUBTC- 393(Lab) [lab On Molecular Biology] Credit2

BSUBTS- 301:Enzymology Credit2

BSUBTS-302 Industrial Biotechnology Credit2

BSUBTS-303 Plant and Animal Chromosome Preparation and Karyotyping Credit-2

1. Basic Principle of Cytogenetics Procedure- Specimen procurement , culture

BSUBTG-3(1,2,3,….n): MOOCS Credit 6