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Injector Lin

The document reports on an injector linearity and accuracy test. The test measured the linearity and accuracy of injections of different volumes of caffeine. The results showed excellent linearity with an R^2 of 1.0 and good accuracy of 0.41 μL, both within specifications.

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Riad Manamanni
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6 views3 pages

Injector Lin

The document reports on an injector linearity and accuracy test. The test measured the linearity and accuracy of injections of different volumes of caffeine. The results showed excellent linearity with an R^2 of 1.0 and good accuracy of 0.41 μL, both within specifications.

Uploaded by

Riad Manamanni
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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INJECTOR LINEARITY & ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Acquired By: ryan


Processing Method: Injector Lin Acc PM Sample Kit 2 Lot Number: 1245
Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Sample Kit 2 Exp. Date: 12/22/2014 12:00:00 AM
Sample Set Id: 4778 Column Serial Number: W1412341AD
Sample Set Name: Injector Lin Acc SSM Result Set Id: 4786
Sample Set Start Date: 12/22/2014 11:30:53 PM EST

TE S T D E S C R I P TI O N
Injector linearity and accuracy is performed to determine whether the injection volume delivered is linear
within the specified criteria. The system makes several injections using four different injection volumes of
one concentration of caffeine from the same vial, and then Empower plots the area against the amount.
The coefficient of determination (R^2) is used to measure the injector linearity and the accuracy is
determined by dividing the y-intercept by the slope.

The qualification passes if the R^2 > or = 0.9990 and Accuracy is < or =1.0 µL.

Caffeine Inj ector Linearity & Accuracy Results


Linearity R^2 Inj ection Accuracy Inj ection Inj ection Accuracy
Linearity R^2 Spec Linearity R^2
Pass/Fail Specification Accuracy Pass/Fail
1 R^2 > or = 0.9990 1.00000 PASS < or = 1.0 ul 0.41 PASS

Printed 10:21:37 AM US/Eastern 12/30/2014 Page: 1 of 3


Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
INJECTOR LINEARITY & ACCURACY REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4778


Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4786

Caffeine Ov erlaid Chromatograms

0.70

0.60

0.50

0.40
AU

0.30

0.20
Caffeine - 0.733

0.10

0.00

0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00
Minutes
SampleName 5 uL
SampleName 20 uL
SampleName 40 uL
SampleName 80 uL

Printed 10:21:37 AM US/Eastern 12/30/2014 Page: 2 of 3


Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
INJECTOR LINEARITY & ACCURACY REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4778


Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4786

Inj e c tion Line a rity Re s ults

Calibration Id: 4803 Y Intercept: -20172

Equation: Y = 4.95e+004 X - 2.02e+004 Slope: 49486

Fit Type: Linear (1st Order) R^2: 1.00000

Caffeine Inj ector Linearity Calibration Plot


4x106

3x106
Area

2x106

1x106

0
0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00
Injection Volume (ul)
R^2 1.00000

Accuracy Calculation: Inj ection Accuracy = |Y intercept/Slope|

Y intercept = -20172 Slope = 49486

Inj ection Accuracy = | -20172 / 49486 | = 0.41

Caffeine Inj ector


Linearity & Accuracy
Summary Results
Inj ection
Linearity R^2
Accuracy
1 1.00000 0.41

Printed 10:21:37 AM US/Eastern 12/30/2014 Page: 3 of 3


Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00

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