CARRYOVER REPORT

ACQUIS IT ION INFORMATION

System Name: 2695_2998_QDa95 Acquired By: SQT_FSE

Processing Method: Carryover PM Sample Kit 2 Lot Number: W23101305
Project Name: SQT_E_2695_2998_QDa_S_RC3 Sample Kit 2 Exp. Date: 4/30/2016 12:00:00 AM EDT
Sample Set Id: 5868 Column Serial Number: 01673419613863
Sample Set Name: Carryover SSM Result Set Id: 5884
Sample Set Start Date: 1/14/2015 9:42:55 AM EST

TEST DESCRIPTION
The Carryover test is performed to determine the presence of sample carryover from one injection to the
next. The test involves running two pre blank (mobile phase) injections to demonstrate that the system is
free of unexplained or "ghost" peaks. This is followed by three injections of a 0.005 % carryover standard
(0.2 ppm caffeine). These injections are followed by a challenge injection (4000 ppm Caffeine), then by
three post-blank injections.

The caffeine peak in the post-blank injection is quantitated against a 0.005 % carryover standard.

The qualification passes if the % carryover is < or = 0.01%, or the peak is missing for PostBlank1.

Carryover Peak Results

Carryover
SampleName Carryover Spec Peak Type % Carryover
Pass/Fail
1 Post Blank 1 < or = 0.01% Found 0.006 PASS

Printed: 2:30:54 PM US/Eastern 1/22/2015 Page: 1 of 3

Waters Project Name and Version: SQT_E_2695_2998_QDa_S Version 1.00
 CARRYOVER REPORT
ACQUISITION INFORMATION

System Name: 2695_2998_QDa95 Sample Set Id: 5868

Project Name: SQT_E_2695_2998_QDa_S_RC3 Result Set Id: 5884

Carryover Chromatograms
0.0002 0.002
0.0004
0.0000
0.0002
-0.0002 0.001
0.0000
AU

AU

AU
Caffeine

Caffeine
-0.0004

Caffeine
-0.0002
0.000
-0.0006
-0.0004
-0.0008
0.00 0.50 1.00 0.00 0.50 1.00 0.00 0.50 1.00
Minutes Minutes Minutes
Sample PreBlank; Inj 1 Sample PreBlank; Inj 2 Sample Carryover Std; Inj 1

0.002 3.00
0.002
2.00
0.001

AU
0.001
AU

AU
Caffeine

1.00
Caffeine

Caffeine
0.000
0.000 0.00
0.00 0.50 1.00
0.00 0.50 1.00 0.00 0.50 1.00 Minutes
Minutes Minutes Sample Carryover Challenge;
Sample Carryover Std; Inj 2 Sample Carryover Std; Inj 3 Inj 1
0.0005
0.002 0.0005

0.0000
0.001
AU

AU

AU
Caffeine

0.0000
Caffeine
Caffeine

0.000 -0.0005
-0.0005

0.00 0.50 1.00 0.00 0.50 1.00 0.00 0.50 1.00

Minutes Minutes Minutes
Sample Post Blank 1; Inj 1 Sample Post Blank 2; Inj 1 Sample Post Blank 3; Inj 1

Printed: 2:30:54 PM US/Eastern 1/22/2015 Page: 2 of 3

Waters Project Name and Version: SQT_E_2695_2998_QDa_S Version 1.00
 CARRYOVER REPORT
ACQUISITION INFORMATION

System Name: 2695_2998_QDa95 Sample Set Id: 5868

Project Name: SQT_E_2695_2998_QDa_S_RC3 Result Set Id: 5884

Chromatographic Peak Results

Sample Type: Unknown
SampleName Sample Type Vial Injection Name RT Area Height Peak Type % Carryover
1 PreBlank Unknown 60 1 Caffeine 0.832 1596 264 Found 0.0008
2 PreBlank Unknown 60 2 Caffeine 0.624 3082 233 Found 0.0015

Chromatographic Peak Results

Sample Type: Standard
SampleName Sample Type Vial Injection Name RT Area Height Peak Type % Carryover
1 Carryover Std Standard 61 1 Caffeine 0.846 9862 2387 Found 0.0050
2 Carryover Std Standard 61 2 Caffeine 0.847 10719 2462 Found 0.0050
3 Carryover Std Standard 61 3 Caffeine 0.849 10690 2455 Found 0.0050

Chromatographic Peak Results

Sample Type: Unknown
SampleName Sample Type Vial Injection Name RT Area Height Peak Type % Carryover
1 Post Blank 1 Unknown 63 1 Caffeine 0.769 12139 2886 Found 0.0058
2 Post Blank 2 Unknown 63 1 Caffeine 0.813 4618 1045 Found 0.0022
3 Post Blank 3 Unknown 63 1 Caffeine 0.826 6701 1328 Found 0.0032

Carryover Summary
Results
SampleName % Carryover
1 Post Blank 1 0.0058

Printed: 2:30:54 PM US/Eastern 1/22/2015 Page: 3 of 3

Waters Project Name and Version: SQT_E_2695_2998_QDa_S Version 1.00