Scribd Logo
Upload

Welcome to Scribd!

  • 11 views

    Replication of DNA

    Uploaded by

    zibnehuq
    The document discusses the process of DNA replication, which produces two identical copies of DNA from one original molecule. It describes three types of replication - semiconservative, conservative, and dispersive. Semiconservative replication is explained in detail, involving the separation of DNA strands, creation of complementary strands through DNA polymerase, and creation of new DNA molecules.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Replication of DNA

    Uploaded by

    zibnehuq
    11 views2 pages
    The document discusses the process of DNA replication, which produces two identical copies of DNA from one original molecule. It describes three types of replication - semiconservative, conservative, and dispersive. Semiconservative replication is explained in detail, involving the separation of DNA strands, creation of complementary strands through DNA polymerase, and creation of new DNA molecules.

    Original Description:

    Dna replication

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document discusses the process of DNA replication, which produces two identical copies of DNA from one original molecule. It describes three types of replication - semiconservative, conservative, and dispersive. Semiconservative replication is explained in detail, involving the separation of DNA strands, creation of complementary strands through DNA polymerase, and creation of new DNA molecules.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    11 views2 pages

    Replication of DNA

    Uploaded by

    zibnehuq
    The document discusses the process of DNA replication, which produces two identical copies of DNA from one original molecule. It describes three types of replication - semiconservative, conservative, and dispersive. Semiconservative replication is explained in detail, involving the separation of DNA strands, creation of complementary strands through DNA polymerase, and creation of new DNA molecules.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Replication of DNA

    It is the process of producing two identical copies from One Original DNA molecule
    Including 3 different types such as—
    1. Semiconservative replication 2.Conservative replication 3. Dispersive
    replication

    Semiconservative replication
    It was proposed by Watson and Crick in 1953. In this process, Produce to copies
    contains one Original and one new strand.It is completed by three consecutive
    stages,

    a) Separation of strands:
    • First of all the DNA is relaxed from its super coiling nature by topoisomerase
    enzyme.
    • Replication begins at the origin of replication along a DNA molecule where
    DNA is unzipped and nitrogen bases separate from each other by helicase
    enzyme forming replication bubbles leading to Y shaped replication fork at
    both ends.
    • SSBP ( single strand binding protein) binds to separated DNA strands and
    prevents them from reannealing after unwinding.

    b)Creation of complementary strands:


    • Separated each strand of DNA serps as a template for synthesis of
    complementary DNA strand
    • Among them one runs from 5’ to 3’ end called leading strand and other runs
    from 3’ to 5’end is called lagging strand .
    • RNA primer made by RNA Primase binds at the end of leading strand and
    various points of lagging strand acting as starting point for DNA synthesis.
    • By the help of DNA Polymerase and magnesium ion several nucleotides are
    attached at the free 3’ end of the leading strand in 5’ to 3’ direction only y the
    help of phosphodiester bond.\
    • In the lagging strand there are discontinuous small fragments of synthesized
    DNA called Okazaki fragments. Later these fragments are joined together by
    DNA Ligase enzyme.In both strands RNA primase adds more RNA primers .

    c) Creation of new DNA:


    • After matching up all the bases an enzyme called exonuclease removes the
    primers then the new strands are proofread to DNA mismatch repair (MMR) in
    the new DNA sequence.
    • Finally DNA Ligase seals the sequence of DNA into two continuous double
    strands.

    You might also like