Journal of Cleaner Production 277 (2020) 122422

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Journal of Cleaner Production

journal homepage: www.elsevier.com/locate/jclepro

Enhanced nitrogen retention by lignite during poultry litter

composting
Yun Cao a, c, d, *, Mei Bai b, Bing Han b, Robert Impraim b, Clayton Butterly b, Hangwei Hu b,
Jizheng He b, Deli Chen b
a
Recycling Agricultural Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China
b
Faculty of Veterinary and Agriculture Science, University of Melbourne, Melbourne, VIC, 3010, Australia
c
Key Laboratory of Crop and Livestock Integrated Farming, Ministry of Agriculture, Nanjing, 210014, China
d
Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing, 210014, China

a r t i c l e i n f o a b s t r a c t

Article history: Composting is an effective method to transform organic wastes into soil amendments. However, sig-
Received 21 November 2019 nificant nitrogen (N) is lost through ammonia (NH3) volatilization during composting. This work eval-
Received in revised form uated the effect of lignite amendment (0% (CK), 5% (T1), 10% (T2), 15% (T3)) on N transformation during
22 March 2020
composting. The results showed T3 increased total nitrogen content by 25% (33.0 g kg
1 in T3 vs
Accepted 20 May 2020
21.0 g kg
1 in CK), and increased mineral N by >60% compared with CK. The X-ray photo-electron
Available online 24 May 2020
spectroscopy and Boehm’s titration analysis showed that composted T3 contained higher total acid
Handling editor: CT Lee groups than composted CK. Notably, the abundances of ammonium oxidizers decreased in all lignite
treatments. High moisture content and low NH3 availability could suppress ammonia oxidizers, therefore
Keywords: improved N retention. Partial least-squares path modeling suggested that physiochemical properties
Brown coal played a dominant role in N loss in composting with or without lignite, however, acid group content
Nitrogen loss appeared to be able to strongly inhibited N loss by adsorption NHþ 4 under composting with lignite
Ammonium oxidizers addition. The results of this study suggested lignite addition at 15% as a proper ratio for composting to
Urease activity
achieve fast organic degradation and great N retention.
X-ray photo-electron spectroscopy
© 2020 Elsevier Ltd. All rights reserved.

1. Introduction 2011). Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing

bacteria (AOB) have been discovered in composting and are sig-
With an increasing number of modern intensive poultry farms, nificant contributors to NH3 oxidation (Yamamoto et al., 2010). Any
the excess of poultry manure in many areas has caused significant NH3 not adsorbed by compost material is rapidly oxidized to NO
2
environmental challenges, in particular, the small areas with via hydroxylamine. Therefore, NH3 oxidation may be restricted by
insufficient surrounding agricultural land for application as a fer- the availability of NHþ
4 in composting.
tilizer. Composting is a cost-effective way to convert poultry litter To reduce nitrous emissions and retain N during poultry litter
into stabilized compost. However, composting of poultry manure composting, diverse disposal strategies including bulking agents,
can cause high loss of nitrogen through ammonia volatilization. In bedding materials, microorganisms, chemicals and various
poultry manure, organic N such as urea N and uric acid N can be amendments such as natural and mineral adsorbents have been
mineralized by microorganisms and converted into ammonium widely used (Awasthi et al., 2019; Wu et al., 2019).
(NHþ 4 ) via ammonification, in which urease catalyzes the hydrolysis Lignite, also known as brown coal, has been used widely as a
of urea to NH3. Reduced urease activity could be related to natural adsorbent and is globally abundant and mined in great
decreased N loss either in soil or composting systems (Cook et al., quantities (Hughes, 2018). It has a similar structure and chemical
composition in terms of C, H, N and O proportion to many biochars
(Paramashivam et al., 2016). Due to its high cation exchange ca-
pacity derived from dissociation of carboxylic and phenolic groups,
* Corresponding author. Recycling Agricultural Research Center, Jiangsu Academy
of Agricultural Sciences, Nanjing, 210014, China. lignite has been used in removal of metal ions, dyes and ammonium
E-mail addresses: [email protected] (Y. Cao), [email protected] from wastewater (Nazari et al., 2018). Granulation of urea with
(D. Chen).

https://doi.org/10.1016/j.jclepro.2020.122422
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Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

lignite delayed mineral N release and maintained higher N in soil rotating to provide fresh air to the composting mixtures. The mass
than urea only (Dong et al., 2009; Saha et al., 2017). of the materials in the composting reactors was weighted at the
More recently, Chen et al. (2015) and Sun et al. (2016a) beginning and the end of the experiment. Compost samples from
demonstrated that lignite application on the surface of cattle pen each reactor were collected on 1, 8, 15, 22, 28, 45 and 65 d and used
decreased NH3 emissions by 38 kg N head
1 y
1 and reduced for the analysis of moisture, pH, electric conductivity (EC), ash,
approximately 30% of the excreted N lost via NH3 volatilization. ammonium and nitrate contents, total carbon (TC), total nitrogen
Based on these studies, the increased N retention in soil and feedlot (TN), seed germination index (GI), urease activity and DNA
systems by lignite application was attributed to: 1) a part of the extraction. Temperatures were measured every day with a portable
NHþ 4 -N generated from organic N mineralization is brought into temperature monitoring probe.
lignite due to the high CEC of lignite or direct binding of NH3 (Chen
et al., 2015); and 2) lignite inhibits the activity of urease, which 2.3. Analytical methods
hydrolyzes urea to NH3, resulting in a lower rate of NH3 emission
(Shen et al., 2016). The decreased availability of NH3 could in turn 2.3.1. Physicochemical analysis of the raw materials and compost
affect the population size or even community structure of ammonia products
oxidizers in compost with consequences for the nitrification pro- Moisture content of fresh samples was measured by oven drying
cess. Lignite may also directly or indirectly influence microbial at 105  C for 24 h. The bulk density was determined using a
communities through increasing water retention and water hold- container with which to apply vertical loads and a platform scale.
ing capacity (Piccolo et al., 1996), decreasing bulk density and The container had an approximate inside diameter of 60 mm and a
improving aeration, retaining nutrients and buffering pH (Dong fill mark of 50 mm from the bottom. The bulk density was calcu-
et al., 2009). Most of the previous studies focused on the effects lated by dividing the dry mass of sample by its volume occupied
of lignite application on NH3 emission or N retention from soil or (Iqbal et al., 2010). Particle size analyses for all samples were per-
pure solution systems, few studies have been conducted to evaluate formed according to Schaub-Szabo and Leonard (1999). The dried
the potential use of lignite as an additive on N transformation compost was sieve-screened to different fractions and weighted.
during composting. The pH and EC were determined from 1:5 (w/v) water-soluble
Therefore, three different ratios (5%, 10% and 15%) of lignite were extract with a pH/EC meter. The water-soluble extract was also
co-composted with poultry litter in a lab-scale composting exper- centrifuged to analyze the water-soluble carbon (DOC) and water-
iment. The objectives of the study were to evaluate the effects of soluble nitrogen (DON) of the compost using a TOC/TN analyzer
lignite amendment on N retention, functional acid group concen- (multi N/C 3000, Analytik Jena AG, Germany). TC and TN were
tration, the abundance of ammonia oxidizers and their correlation analyzed on a LECO Trumac CN at a furnace temperature of 1350  C.
with physiochemical characteristics during poultry litter com- Dried samples were put into a muffle furnace at 550  C for 24 h for
posting. The novelty of the study aimed to provide evidence for the ash content calculation. Ammonium (NHþ

4 ) and nitrate (NO3 ) was
potential use of lignite as an additive for the reduction of N loss extracted with 2 M KCl (1:20) and analyzed on a Skalar SANþþ
during composting of agricultural wastes. Meanwhile, changes of segment flow analyzer (TrACESS Soil Node, University of Mel-
functional acid group and evolution of ammonifiers during com- bourne). Phytotoxicity of compost samples to the lettuce was car-
posting were analyzed to elucidate the mechanism of N retention ried out using the method provided by Zucconi et al. (1981). The
by addition of lignite during composting. cation exchange capacity (CEC) of composts was measured by the
method of ammonium acetate (Ciarkowsk et al., 2017). The oxygen-
2. Materials and methods containing acidic surface functional groups of fresh and composted
samples were determined by the method of Boehm’s titration
2.1. Description of composting feedstocks (Boehm, 1994).

Poultry litter was collected from a commercial broiler house 2.3.2. Urease activity measurement
near Victoria, Australia. The initial bedding material was composed Urease activity was determined using the Urease Activity Assay
of sawdust, straw and rice husk. The lignite was Bacchus Marsh Kit (Sigma-Aldrich) according to the manufacturer’s instruction.
brown coal obtained from Victoria, Australia. One unit of urease is the amount of enzyme that catalyzes the
formation of 1.0 mmol NHþ 4 h

1
at pH 7.0.
2.2. Composting process
2.3.3. DNA extraction and quantitative PCR in compost samples
The composting was performed at the Animal House at the Samples collected on 1, 8, 28 and 65 d representing the initial
University of Melbourne, Australia. Poultry litter was sieved with stage, thermophilic stage, cooling stage and maturity stage,
dimensions from 1e2 cm. The lignite was screened with a 2-mm respectively, were used for downstream molecular analysis.
sieve. Previous study showed that lignite added at the rate of Genomic DNA was extracted from 0.25 g of frozen compost sample
6 kg m
2, which was equivalent 10% to the total dry weight, could using FastDNA SPIN KIT for soil (MP Biomedicals, Solon, OH, USA)
enhance mineral N retention in cattle manures in feedlots signifi- according to the manufacturer’s instructions. After extraction, the
cantly (Shen et al., 2016). As a result, poultry litter was mixed with DNA was purified by spin-bind cartridges after being precipitated
lignite at 0 (CK), 5% (T1), 10% (T2) and 15% (T3) to the total dry by PEG 6000 (Yang et al., 2007). The abundances of bacterial 16S
matter. Each treatment was adjusted to a final moisture content of rRNA, AOA and AOB amoA genes were examined by quantitative
55e60% and had two replicates. PCR using the primer pairs and amplification conditions according
The raw materials of about 50 kg were transferred to commer- to previous research (Shi et al., 2016).
cial composting reactors. Each tumbling composter, insulated with
10-cm thick glasswool, had a volume of 160 L with a size of 725 mm 2.3.4. Spectroscopic characterization of composts by X-ray photo-
(width)  935 mm (height)  660 mm (length). At 30 cm from the electron spectroscopy (XPS)
bottom of both sides of the reactor, there were twelve 1  1 mm2 XPS was performed on a Kratos Axis ULTRA X-ray Photoelectron
holes which allowed aeration from the left and right sides. The Spectrometer using monochromatic Al Ka X-rays (1486.6 eV) at
composting reactors were turned once every day by manually 150 W (15 kV, 15 ma) under base pressure of s 1.0  10
8 torr. Pass
2
Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

energy of 160 eV for survey (wide) scans and multiplex (narrow)

scans at 20 eV was used. Scanned volume was ~2400 cubic micron
with an area of 0.8  0.3 mm2 and a depth of 10 nm. Survey scans
and narrow high-solution scans were carried out over 1200-0 eV
binding energy range with 1.0 eV steps and 0.05 eV steps, respec-
tively. Data analysis, curve fitting and calibration was carried out on
CasaXPS software.

2.4. Statistical analyses

Statistical analyses were carried out on the IBM SPSS Statistics

19 (IBM Corporation, Armonk, NY, USA). Redundancy analysis
(RDA) was performed to analyze the relationships between the
physicochemical characteristics and the population of AOA and
AOB. Variance partitioning was used to assess the variations in the
abundances of AOA and AOB explained by various physicochemical
properties. RDA and variance partition were performed using the
Canoco 5.0 (Microcomputer power, Ithaca, NY, USA). Partial least
Fig. 1. Temperature profiles of four compost piles over the 65-d study. CK: poultry
squares path modeling (PLS-PM) was used to examine the complex
litter with no lignite, T1: poultry litter with 5% lignite; T2: poultry litter with 10%
relationships among various variables on N loss using the R package lignite; T3: poultry litter with 15% lignite.
plspm (v 0.4.7). The physicochemical properties (pH, EC, ash, DOC,
CEC, moisture content, TC, TN, NHþ 4 ), total bacterial abundance,
ammonia oxidizers abundance and total acid group contents (-OH,
COO, C]O) were included in the model. By reducing the bulk density of compost material, the lignite
addition may improve aeration in the feedstocks that promotes
3. Results and discussion microbial activity and substantially improve the degradation of the
OM (Jain et al., 2019).
3.1. Impact of lignite on the temperature variation during Lignite addition changed moisture variation during composting.
composting process The moisture content of the mixtures decreased from 64.0% to
60.4% for CK and from 63.6% to 59.9% for T1, however, the moisture
The temperature in all treatments followed the typical evolution content for T2 and T3 showed an upward trend during composting
of composting process, heating stage (d 1~ d 2), thermophilic stage (Table 1). The higher moisture content in T2 and T3 than that in CK
(d 3~ d 12), cooling stage (d 13~d 28) and maturity stage (d 29 ~ d could be attributed to higher microbial metabolic activity in OM
65) (Fig. 1). The temperatures increased quickly in all treatments degradation during composting. Furthermore, composting vessels
and reached the maximum on day 2: 64.80  C (CK), 67.05  C (T1), without forced ventilation was used in the present study, so the
69.45  C (T2), 69.45  C (T3). The faster increase of temperature in water reduction would be reduced. The increase in the moisture
the heating stage and longer thermophilic phase indicated that content could be due to the water uptake and water holding ca-
addition of lignite improved the performance the composting pacity of lignite (Cihlar et al., 2014).
process (Fig. 1). Thermophilic phase (compost temperature >55  C) Particles of different sizes formed progressively in all treatments
in the study was extended by 3e7 d for the mixtures amended with throughout composting regardless of turning. The average weight
lignite: i.e. 3, 9, 7 and 8 d for CK, T1, T2 and T3, respectively. Hence, percentage corresponding to the fractions >3, 1e3 mm and <1 mm
the addition of lignite to the poultry litter resulted in the extension was 61.21%, 28.97% and 10.23% for CK and 83.76%, 12.63% and 3.61%
of the thermophilic stage of composting time. Higher temperatures for T3, respectively. Therefore, lignite addition at 15% level
observed at the beginning of the process and a longer increased the generation of large particles, thus decreased the ratio
thermophilic-active phase in the mixtures amended with lignite of small (<1 mm) compared with CK. In fact, large clumps (>1 cm)
could accelerate the OM decomposition, and consequently shorten accounted for 35.2% (dry weight) of the total particles (>3 mm) in
the time required for maturity. Lignite can be a habitat for micro- T3 compared to 8.7% large clumps (>1 cm) in CK. The reduced bulk
organisms due to its large surface area and strong water retention density and high moisture content of T3 treatment resulted in
capacity (Kolb et al., 2009). The results were similar to Georgacakis significant leachate formation, leading to the formation of large
et al. (1996), where lignite amendment resulted in prolonged clumps at the curing stage. The formation of large clumps can
thermophilic stage and improved compost quality during solid facilitate the reduction in porosity and oxygen availability which
swine manure composting. would in turn inhibit the growth of microorganisms (Jain et al.,
2019), and was supported by a decrease in copy numbers of the
3.2. Impact of lignite on the evolution of physicochemical 16S rRNA gene, AOA amoA and AOB amoA genes recorded in T3 on
characteristics during composting day 65.
The initial CEC values were significantly higher in T2 and T3 than
Physical properties (bulk density, particle sizes, and moisture in CK and T1 due to the higher rates of lignite added. In all treat-
content) significantly influence the performance of composting ments, the CEC increased dramatically in the first week and then
(Richard et al., 2002). At the beginning of composting, the addition increased gradually until the end of the study (Table 1). The
of lignite at 10e15% significantly reduced the bulk density, which significantly higher CEC in T3 and T2 could be due to the higher
might be due to the high porosity of lignite (Yue et al., 1999). As contents of carboxyl and phenolic functional groups in the lignite
composting progressed, the bulk density of all treatments (Benito et al., 2003). The initial pH in the compost mixtures was
increased and showed no significant difference among treatments significantly decreased by the addition of lignite and the lowest pH
(Table 1). The greatest increase in a percentage of bulk density and was observed in T3 (pH 7.91) compared to CK (pH 8.19) (Fig. 2A). As
decrease in the volume of raw material was observed for T3 (42%). the composting progressed, the pH in lignite added mixtures was
3
Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

consistently lower than CK. In the initiation and thermophilic

114.1(2.5)b
122.7(2.7)a
stages of composting, no significant difference was observed in EC

87.9(5.6)c
96.9(3.6)c
between treatments. However, the EC was significantly lowered by

d 65
lignite addition in the cooling and maturity stages (Fig. 2C). The
reduction of pH and EC in the mixtures of T3 could be related to the
low pH and high contents of carboxyl and phenolic groups

116.0(5.3)a
118.7(4.5)a
79.4(4.2)b
87.3(3.1)b
providing the active sites for ion exchange (Qi et al., 2011). The
cation exchange capability of lignite could contribute to the
d45
removal of cations from aqueous solution. However, DOC concen-
Cation exchange capacity (cmol/kg)

trations decreased gradually from 14.5e19.4 g kg
1 at the begin-

ning to 9.2e10.7 g kg
1 at the end of the composting amended with
114.7 (5.0)a
109.6(4.5)a
different ratios of lignite (Fig. 2E). The conditions were favorable for
81.3(2.5)b
89.7(5.6)b

microbes in T1, T2 and T3 to utilize DOC and proliferate, resulting in

sustained decreasing of DOC during the composting process
d8

(Castaldi et al., 2008). The reduction of DOC could also be attributed

to the absorption of DOC by lignite (Nazari et al., 2018). However,
66.6(1.3)ab

the DOC content in CK reached a peak on day 28 (23.2 g kg
1) and

69.2(1.4) a
65.1(0.8)b

71.6(2.6)a

decreased to 9.3 g kg
1 by day 65. The increase of DOC in CK sug-

gested that the degradation and utilization of DOC was much
d1

slower than the rate of synthesis. Although not quantified in this

study, this result could be associated with the lower microbial ac-
63.1(1.2)ab

Means (standard error) (n ¼ 3) within columns followed by different lowercase letters are significantly different at P ¼ 0.05 as determined by LSD test.
60.4(0.2)b
59.9(2.8)b

65.5(2.9)a

tivities in CK compared to lignite-amended treatments during the

first 28 d (Liu et al., 2011). The variation in DON contents in CK and
d 65

treatments showed a similar trend, increasing gradually during the

first 28 d, reaching maximum value on day 28 and then decreasing
slowly until the end of the experiment (Fig. 2F). DON and NHþ 4 -N
60.2(0.3)b
60.1(0.2)b
65.8(0.5)a
65.9(0.4)a

showed a similar trend and was inversely proportional to TN. It

could be inferred that TN was quickly transformed into DON and
Moisture content (%)

d45

NHþ 4 -N. The GI values suggested that lignite could enhance the
maturity of the compost (Fig. 2 B). GI dropped from 0.23e0.49 on
day 1 to 0.08e0.2 on day 22. However, the GI in T3 was increased to
60.1(1.7)ab
62.8(3.3)ab
58.9(2.4)b
65.9(2.9)a

CK, poultry litter only; T1, poultry litter þ 5% lignite; T2, poultry litter þ 10% lignite; T3, poultry litter þ 15% lignite.

0.63 on 28 d and kept rising afterwards, while it was only increased

to 0.32 on 65 d. The TC content decreased with composting time in
d8
Variation in bulk density and moisture content of different composting materials during composting process.

all the mixtures (Fig. 2D). Greater OM-loss of 51.92% and 49.17% was
observed for T3 and T2 compared with 39.78% and 42.44% for T1
59.1(2.6)ab

and CK, respectively. Therefore, the lignite addition at 15% level had
58.1(2.4)b
64.0(2.8)a
63.6(2.5)a

the greatest impact on the overall OM degradation. The higher OM

losses in T3 can be partly attributed to higher temperature and
d1

longer thermophilic stage compared to other treatments during

composting. The C/N ratio increased rapidly from during the first 8
0.193(0.05)a
0.196(0.04)a
0.188(0.05)a
0.182(0.01)a

days, after which the value remained stable in CK, in lignite-added

piles, however, the C/N decreased gradually from day 8 until the
end of composting (Fig. 3A). The decrease of C/N was more rapid
d65

and pronounced in T3, which should be caused by the decrease in

the content of TC and the increase in the TN.
0.182(0.04)a
0.183(0.02)a
0.173(0.05)a
0.162(0.06)a

3.3. Impact of lignite on N transformation

d45
Bulk density (g cm
3)

Lignite addition improved N retention in compost. The NHþ 4

concentration in all treatments increased to a peak of 7.6, 7.7, 6.6
and 7.2 g NH4eN kg
1 for CK, T1, T2 and T3, respectively (Fig. 3B).
0.138(0.02)b
0.128(0.01)b
0.166(0.08)a
0.153(0.04)a

This reflects higher organic N ammonification rate driven by

intense microbial activity during the heating and thermophilic
stage. The increase in NHþ4 concentration might be due to increased
d8

temperature which favored the mineralization of organic nitrogen

compounds. In the curing stage, NHþ 4 concentration was signifi-
0.132(0.02)b
0.123(0.04)b
0.160(0.03)a
0.151(0.04)a

cantly higher in the mixtures with 10% (2.1 g kg
1) and 15%
(2.5 g kg
1) than that of 5% (1.9 g kg
1) and CK (1.5 g kg
1). The
higher NHþ 4 concentration in T3 can be attributed to the strong
d1

adsorption capacity of NHþ
1

4 (76.7 cmol kg ) by lignite. Nitrification
was quite weak during the composting process. The initial nitrate
concentration was below 4 mg kg
1 for all mixtures and increased
Treatment

to 19, 15, 23 and 5 mg kg
1 for mixtures with 0%, 5%, 10% and 15%
Table 1

lignite, suggesting that higher rates of lignite decreased the gross

CK
T1
T2
T3

nitrification rate. The low rate of nitrification in T3 may be

4
Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

Fig. 2. Changes in physico-chemical properties of four compost piles. CK: poultry litter with no lignite, T1: poultry litter with 5% lignite; T2: poultry litter with 10% lignite; T3:
poultry litter with 15% lignite over the 65-d study.

attributed to unavailability of strongly adsorbed NHþ4 to ammonium 3.4. Characterization of functional groups of the fresh and
oxidizers (Mahimairaja et al., 1994). These results were comparable composted mixtures
with that reported by Patti et al. (1992) that brown coal when
applied to soil and can act as nitrification inhibitors. The nitrifica- Table 3 shows the total acidity of three main functional groups
tion inhibition during composting could further reduce the po- (carboxyl/lactonic/phenolic groups) of lignite, fresh and com-
tential for N loss through nitrate leaching. posted samples. The acidity in lignite and compost samples was
The TN decreased markedly from 26.0, 24.7, 24.3 g kg
1 to 21.0, due to the carboxyl groups, lactones and phenols (Boehm, 1994).
22.3, 23.7 g kg
1 for mixtures with 0, 5% and 10% lignite, respec- The results revealed that the concentrations of acidic functional
tively (Fig. 3E). However, TN in T3 treatment was increased signif- groups increased gradually during composting, the total acidity
icantly from 24.7 to 33.0 g kg
1. The significant increase of TN in T3 increased from 1.74 to 2.17 and 1.86e2.46 mmol g
1 in CK and T3,
could be partly due to the concentration effect provoked by the OM respectively. The increase in the total acidity might be due either
biodegradation (weight loss). The minimum N loss was recorded in to the oxidation of methoxyl and alcoholic groups of side chains of
T3 and was 25% lower than CK (Table 2). The parameters of kinetic lignin or to the microbial degradation of carbohydrates to
equations also suggested lower TN loss for mixture with 15% lignite carboxyl, phenolic and ketone groups (Lou et al., 2015). The higher
during the composting process. concentrations of acidic functional groups in T3 than CK should be
Higher temperature and pH of the mixtures favor NH3 volatili- attributed to the addition of lignite, which contain more lactones
zation. However, the temperature in lignite amended mixtures and phenols than poultry litter, indicating that it would be
increased and the pH was not significantly changed by the addition favorable for the degradation of OM and enhancing the microbial
of lignite during the thermophilic stage of composting. Therefore, activity in lignite-poultry litter mixtures composting (Zhang et al.,
higher N retention in lignite amended mixtures could not be 2014).
explained by the temperature or pH increase. In fact, the NHþ 4 As significant difference in N retention was only observed be-
produced from organic N mineralization during composting could tween CK and T3, which was discussed above, the oxygen-
be partially captured by lignite, since lignite amendment increased containing acidic functional groups of fresh and composted sam-
the CEC and the NHþ 4 -N could be captured by the abundant func- ples in CK and T3 were further characterized by XPS. The com-
tional groups such as eCOOH and eOH (Paramashivam et al., 2016), posted poultry litter (composted CK) had a higher relative
which will be discussed later. concentration of CeO (alcoholic, phenolic, hydroxyl) and C]O
(ketones and quinones) but lower concentrations of CeC and

Fig. 3. Changes in NHþ4 -N and TN of four compost piles. CK: poultry litter with no lignite, T1: poultry litter with 5% lignite; T2: poultry litter with 10% lignite; T3: poultry litter with
5% lignite over the 65-d study.

5
Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

Table 2
Nitrogen losses from different treatments during composting.

Treatment TN before composting (kg/reactor) TN retained in the end (kg/reactor) TN loss (kg/reactor) TN loss (%)

CK 0.54 (0.04)a 0.26 (0.02)b 0.28 (0.02)a 52.0 (2.8)a

T1 0.51 (0.02)b 0.26 (0.01)b 0.25 (0.02)b 49.0 (3.3)a
T2 0.49 (0.02)b 0.25 (0.01)b 0.24 (0.02)b 48.9 (3.4)a
T3 0.50 (0.02)a 0.29 (0.02)a 0.21 (0.03)b 42.4 (3.2)b

Values in parentheses are standard errors (n ¼ 3).

Values in a column with different letters indicate significant difference at P < 0.05 according to LSD test.

Table 3
Acid functional groups of composts determined by Boehm’s titration method.

Sample Total acidity (mmol g
1) Carboxylic groups (mmol g
1) Lactonic groups (mmol g
1) Phenolic groups (mmol g
1)

Poultry litter
d1 1.74 0.90 0.21 0.63
d8 1.98 1.05 0.22 0.71
d28 2.07 1.08 0.24 0.75
d65 2.17 1.12 0.24 0.81
Poultry litter þ 15% lignite
d1 1.86 0.82 0.33 0.71
d8 2.07 0.86 0.37 0.89
d28 2.21 0.91 0.39 1.04
d65 2.46 0.92 0.41 1.13

Lignite 2.7 0.7 0.8 1.2

lower total C 1s compared to fresh poultry litter (fresh CK). 3.5. Lignite effects on the abundance of 16S rRNA gene and
Furthermore, the atomic concentrations of eOH and C]O ammonia oxidizers and urease activity
increased by 74% and 132% in composted CK, and by 82% and 253%
in composted T3, respectively. These results confirm the The 16S rRNA gene copy numbers of total bacteria and the amoA
increasing density of acidic functional groups on the surface of gene copy numbers of AOB and AOA in the mixtures are presented
composted materials. in Fig. 4. The 16S rRNA gene was the most abundant during the

Fig. 4. Abundances of A) AOB amoA gene, B) AOA amoA gene, C) 16S rRNA gene and D) urease activity in the composts without lignite (CK) or with 5% (T1), 10% (T2) and 15% (T3)
lignite after 1 (D1), 8 (D8), 28(D28) and 65 (D65) d composting.

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Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

whole composting process and ranged between 1.6  1012 to

7.4  1012 copies g
1 DW compost sample. It did not vary signifi-
cantly between samples during the active fermentation phase.
However, the 16S rRNA gene abundance increased sharply and was
1.2e1.8 times higher in the mixtures from T2 and T3 collected on
day 8 compared to those collected on day 1. In the cooling stage
(D28), the 16S rRNA gene abundance was significantly higher than
CK and those with lower doses of lignite, indicating that lignite at
particular levels could enhance the growth of microorganisms,
probably due to greater water retention capacity creating a favor-
able micro-environment for bacteria (Richard et al., 2002). The AOA
and AOB were abundant throughout the entire composting process.
AOB abundances were generally higher than AOA in mixtures
regardless of lignite addition. The abundances of AOB and AOA were
in the range of 1.67  108e7.77  108 and 1.03  107e4.31  108
copies g
1 DW, respectively. The significantly lower AOB abundance
in lignite added treatments in the thermophilic stage could be
attributed to the higher temperature stress on the community. T2
had the highest copy of AOA, although significantly higher tem-
perature in T2 (55.6  C) was recorded than CK (46.6  C) on day 8.
The high density of AOA in the high temperature could be attrib-
uted to the reproduction thermophilic ammonia oxidizers (Awasthi
et al., 2018). These results are consistent with previous studies,
which demonstrated that AOA have tolerance to relatively high
temperature (Zeng et al., 2011; Li et al., 2019). The application of
lignite also inhibited AOB abundance in the cooling and maturing
phase of composting. Mixtures with 15% lignite significantly
inhibited AOA abundance by 30% on day 45 and the inhibition rate
increased to 54%, 78% and 86% for mixtures with 5%, 10% and 15%
lignite on day 65, respectively. The inhibition of lignite on the
growth of AOA and AOB could be due to lower free substrate (NHþ 4)
concentration in T3, as a large proportion of NHþ 4 could be captured
by the acidic functional groups on the surface of lignite. Another
possible reason for the growth inhibition of AOA and AOB might be
related to the higher moisture content in compost materials from
T3. Increased moisture content could decrease the O2 penetration
and thus inhibit the nitrification (Guo et al., 2016; Sun et al., 2016b).
The inhibition on the nitrification in the curing stage could be the
Fig. 5. Redundancy analysis (RDA) of physicochemical properties (TC, TN, NHþ 4 -N,CEC,
main reason for the low nitrate content in mixture with 15% lignite. DOC, DON, ash, moisture, pH and GI) (a) and relationship between the relative
Urease is responsible for the breakdown of urea into NHþ 4 that abundances of the AOA and AOB and environmental parameters (b) in compost
can be easily lost as NH3 along with high pH and temperature samples collected at d1, d 8, d28 and d 65 during composting. TC, total organic carbon;
(Castaldi et al., 2008). Low urease activity results in low NH3 TN, total nitrogen; CEC, cation exchange capacity; DOC, dissolved organic carbon; DON,
dissolved organic nitrogen; GI, germination index.
volatilization during the process (Kong et al., 2018). The urease
activity in all mixtures decreased significantly on day 8 compared
to day 1. However, urease activity increased sharply from 0.99 mmol
NH3 g
1 h
1 on day 8 to 3.03 mmol NH3 g
1 h
1 on day 65 in CK, physicochemical properties of poultry litter mixtures after 65-
while it changed slightly for mixtures with 5e15% lignite (Fig. 4D). d composting.
The results are in line with those obtained by Liu et al. (2011), who Environmental factors may play a crucial role in the growth of
reported that the increase in urease activity was attributed to the AOB and AOA during composting. The first two canonical axes
increased availability of water soluble NHþ 4 , which could be explained 57.5% and 26.7% of the variation in the abundances of
absorbed by the acid functional groups of the lignite, resulting in total bacteria, AOA and AOB. TN, GI, pH, moisture, ash, and CEC
suppressed urease activity. contributed significantly (P < 0.05) to the amoA gene abundance-
environment relationship (Fig. 5B). Forward selection analysis
3.6. Correlations between environmental factors and ammonia showed that TN, moisture and CEC explained 28.4% (P < 0.01), 23.7%
oxidizers (P < 0.05) and 20.1% (P < 0.05) of the total variation. DOC and NHþ
4-
N were significantly positively related to AOA, while CEC was
A total of 11 environmental factors (moisture, TC, TN, ash, DOC, significantly negatively related to AOA. The increase in CEC during
DON, GI, pH, CEC and NHþ 4 -N) were selected as independent pa- composting could be attributed to the accumulation of lignin-
rameters for RDA analysis (Fig. 5A). The first two axes of the RDA derived substances and the increased contents of carboxyl and/or
explained 87.2% of the cumulative variances. Based on the result phenolic hydroxyl groups (Satisha and Devarajan, 2007). Moisture
from RDA, the variation of physicochemical properties was more was negatively related to AOA, AOB and 16s rRNA. The pH values
closely related to the stage of composting rather than the type of had significantly positive correlation with AOB while TN was
raw materials for samples collected at day 1, 8 and 28. However, the negatively correlated to the abundances of AOB. High pH in the
end products were separated from each other. These results sug- active phase could enhance NH3 emission that would also remove
gested that the addition of 15% lignite significantly changed the the possible excessive amount of NHþ 4 that would inhibit the

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Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

Fig. 6. Direct and indirect effects of different factors on total N loss during composting with or without lignite. A, B: PLS-PM showing the relationships among physicochemical
properties (pH, EC, ash, DOC, CEC, moisture content, TC, TN, NHþ 4 ), bacterial abundance (16s rRNA gene copy number), ammonifier abundance (AOA and AOB abundances) and
functional acid group contents (-OH, COO, C]O) with respect to total N loss during composting without (A) and with 15% lignite (B). The positive and negative effects are indicated
by blue and orange arrows, respectively. Larger path coefficients are indicated by wider arrows. Significance levels of Path coefficients are indicated by * (P < 0.05) and **(P < 0.01).
C, standardized direct and indirect effects estimated by PLS-PM in composts without (C) or with 15% lignite (D).

activity and growth of AOB (C aceres et al., 2018). High water con- strongly inhibited N loss by more intensified absorption of NHþ 4,
tent may influence gaseous exchange by limiting diffusion and this which in turn had an inhibitory effect on the growth of ammoni-
restricts oxygen utilization by microorganisms including ammonia fication oxidizers. In contrast, in compost without lignite, the
oxidizers. This would in turn inhibit nitrification (Onwosi et al., ammonifiers abundance could be more strongly affected by phys-
2017). iochemical properties. These results further supported the idea that
apart from the physiochemical properties, the N loss was strongly
3.7. Relative contributions of selected factors to total N loss during decreased by the adsorption of NHþ 4 by the functional acid groups of
composting lignite and the associated decreased levels of ammonification
oxidizers.
Physicochemical properties, microbial abundance and func- Given the market price for urea fertilizer (46% N) of $600 AUD
tional acid group contents determined by Boehm’s titration could t
1, the increased N nutrient in the compost by lignite is equivalent
directly or indirectly influence the total N loss during composting, a to approximately $1.8 AUD and the cost of 15% lignite application as
PLS-PM was constructed to explore how these factors contributed compost additive to be $1.2 AUD when composting of 1 t poultry
to total N loss (Cui et al., 2019). The physiochemical property was litter. The addition of lignite is a cost-effective method for reducing
the key factor affecting the total N loss in composting with or N loss during composting and improve the quality of the final
without lignite (Fig. 6). The primary factor of physiochemical composts. However, further studies are needed to evaluate the
properties could directly influence the N loss and affect the abun- environmental impacts of lignite as a compost additive by
dances of total bacteria and ammonifiers, eventually impact the TN measuring the gaseous emissions of NH3 and N2O directly during
loss. These results could be related to the fact that the physi- composting.
ochemical can directly influence the microbial abundances and
community structure (Zeng et al., 2011). In both treatments, 4. Conclusions
physicochemical properties and total bacterial abundance had
positive effect on TN losses, even though with different magnitude Adding 15% lignite (T3) to poultry litter significantly increased
(Fig. 4). Although the ammonifier abundance and acid group con- the temperature in the thermophilic stage of composting, leading
tent had a minor influence on TN loss compared to other factors, it to faster degradation of OM and accelerated detoxification than
had opposite roles in different treatments. These results suggested composting without lignite (CK). The results indicate that lignite
that the underlying mechanisms towards N loss during composting amendment increased NHþ 4 content by 66% and decreased TN loss
with or without lignite could be different (Liao et al., 2018). In by 18%. The higher concentration of NHþ 4 corresponds with higher
compost with 15% lignite, acid group content appeared to be more TN content (3.2% in T3 vs 2.2% in CK) and higher concentration of
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Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

total acid groups in T3 (2.46 mmol g
1) than those in CK effective method to mitigate ammonia loss from intensive cattle feedlots:
(2.17 mmol g
1). Decreased urease activity and abundance of application of lignite. Sci. Rep. 5, 16689.
Ciarkowsk, K., Podwik, K.S., Filipek-Mazur, B., Tabak, M., 2017. Comparative effects
ammonia oxidizers caused by excessive NHþ 4 -N inhibition and of lignite-derived humic acids and FYM on soil properties and vegetable yield.
moisture content were observed in T3. Although physicochemical Geoderma 303, 85e92.
Cihlar, Z., Vojtova, L., Conte, P., Nasir, S., Kucerík, J., 2014. Hydration and water
properties were the key factor for N loss emission in all composting
holding properties of cross-linked lignite humic acids. Geoderma 230e231,
treatments, the N loss was strongly inhibited by NHþ 4 adsorption by 151e160.
functional acid groups in compost with lignite. This study provides Cook, K.L., Rothrock Jr., M.J., Eiteman, M.A., Lovanh, N., Sistani, K., 2011. Evaluation of
new evidence for the potential use of lignite as a cost-effective nitrogen retention and microbial populations in poultry litter treated with
chemical, biological or adsorbent amendments. J. Environ. Manag. 92, 17600-
compost additive to enhance N retention during composting. The 1766.
hydrophilic property of lignite involved in reduced N loss and the Cui, P., Chen, Z., Zhao, Q., Yu, Z., Yi, Z., Liao, H., Zhou, S., 2019. Hyperthermophilic
effect of lignite on direct gaseous N emissions during composting composting significantly decreases N2O emissions by regulating N2O-related
functional genes. Bioresour. Technol. 272, 433e441.
needs to be further studied. Dong, L., Co rdova-Kreylos, A.L., Yang, J., Yuan, H., Scow, K.M., 2009. Humic acids
buffer the effects of urea on soil ammonia oxidizers and potential nitrification.
CRediT authorship contribution statement Soil Biol. Biochem. 41, 1612e1621.
Georgacakis, D., Tsavdaris, A., Bakouli, J., Symeonidis, S., 1996. Composting solid
swine manure and lignite mixtures with selected plant residues. Bioresour.
Yun Cao: Conceptualization, Data curation, Formal analysis, Technol. 56, 195e200.
Writing - original draft. Mei Bai: Investigation, Methodology, Re- Guo, X., Lu, Y., Li, Q., 2016. Effect of adding flue gas desulphurization gypsum on the
transformation and fate of nitrogen during composting. Compost Sci. Util. 24
sources, Software. Bing Han: Methodology, Resources. Robert (4), 230e237.
Impraim: Methodology, Software. Clayton Butterly: Writing - re- Hughes, A., 2018. Australian Resource Reviews: Brown Coal 2017. Geoscience
view & editing, Validation. Hangwei Hu: Methodology, Writing - Australia, Canberra.
Iqbal, M.K., Shafiq, T., Ahmed, K., 2010. Characterization of bulking agents and its
review & editing. Jizheng He: Supervision, Project administration. effects on physical properties of compost. Bioresour. Technol. 101, 1913e1919.
Deli Chen: Funding acquisition, Supervision. Jain, M.S., Paul, S., Kalamdhad, A.S., 2019. Utilization of Biochar as an amendment
during lignocellulose waste composting: impact on composting physics and
Realization (probability) amongst physical properties. Process Saf. Environ. 121,
Declaration of competing interest 229e238.
Kolb, S.E., Fermanich, K.J., Dornbush, M.E., 2009. Effect of charcoal quantity on
The authors declare that they have no known competing microbial biomass and activity in temperate soils. Soil Sci. Soc. Am. J. 73,
1173e1181.
financial interests or personal relationships that could have Kong, Z., Wang, X., Liu, Q., Li, T., Chen, X., Chai, L., Liu, D., Shen, Q., 2018. Evolution of
appeared to influence the work reported in this paper. various fractions during the windrow composting of chicken manure with rice
chaff. J. Environ. Manag. 207, 366e377.
Li, X., Shi, X.S., Lu, M.Y., Zhao, Y.Z., Guo, R.B., Peng, H., 2019. Improved nitrogen
Acknowledgements conservation capacity during composting of dairy manure amended with oil
shale semi-coke as the porous bulking agent. J. Hazard. Mater. 388, 121742
This work was financially supported by a Cooperative Research https://doi.org/10.1016/j.jhazmat.2019.121742.
Liao, H., Lu, X., Rensing, C., Friman, V.P., Geisen, S., Chen, Z., Yu, Z., Wei, Z., Zhou, S.G.,
Centres Project (CRC-P), the National Natural Science Foundation of Zhu, Y.G., 2018. Hyperthermophilic composting accelerates the removal of
China (41701340), the National Key Technology R & D Program antibiotic resistance genes and mobile genetic elements in sewage sludge.
(2016YFD0501401), the Jiangsu Academy of Agricultural Sciences Environ. Sci. Technol. 52, 266e276.
Liu, D., Zhang, R., Wu, H., Xu, D., Tang, Z., Yu, G., Xu, Z., Shen, Q., 2011. Changes in
Research Foundation (027026111631), and Jiangsu Government biochemical and microbiological parameters during the period of rapid com-
Scholarship for Overseas Studies (JS-2017-134), China. We also posting of dairy manure with rice chaff. Bioresour. Technol. 102 (19),
thank TrACEES platform University of Melbourne for sample anal- 9040e9049.
Lou, L., Liu, D., Chen, H., Chen, F., He, Y., Tian, G., 2015. The change of organic matter
ysis. We specially thank Dr. Haigang Guo from Hebei University of
in sewage sludge composting and its influence on the adsorption of penta-
Engineering and Dr. Yushu Zhang from Fujian Academy of Agri- chlorophenol (PCP). Environ. Sci. Pollut. Res. 22, 4977e4984.
cultural Sciences for their great help in the setting up of the com- Mahimairaja, S., Bolan, N.S., Hedley, M.J., 1994. Losses and transformations of ni-
posting experiment. trogen during composting of poultry manure with different amendments: an
incubation experiment. Bioresour. Technol. 47, 265e273.
Nazari, M.A., Mohaddes, F., Pramanik, B.K., Othman, M., Muster, T., Bhuiyan, M.A.,
Appendix A. Supplementary data 2018. Application of Victorian brown coal for removal of ammonium and or-
ganics from wastewater. Environ. Technol. 39 (8), 1041e1051.
Onwosi, C.O., Igbokwe, V.C., Odimba, J.N., Eke, J.E., Nwankuwoala, M.O., Iroh, I.N.,
Supplementary data to this article can be found online at Ezeogu, I., 2017. Composting technology in waste stabilization on the methods,
https://doi.org/10.1016/j.jclepro.2020.122422. challenges and future prospects. J. Environ. Manag. 190, 140e157.
Paramashivam, D., Clough, T.J., Carlton, A., Gough, K., Dickinson, N., Horswell, J.,
Sherlock, R.R., Clucas, L., Robinson, B.H., 2016. The effect of lignite on nitrogen
References mobility in a low-fertility soil amended with biosolids and urea. Sci. Total En-
viron. 543, 601e608.
Awasthi, M.K., Chen, H., Liu, T., Awasthi, S.K., Wang, Q., Ren, X., Duan, Y., Zhang, Z., Patti, A.F., Verheyen, T.V., Douglas, L., Wang, X., 1992. Nitrohumic acids from
2019. Respond of clay amendment in chicken manure composts to understand Victorian brown coal. Sci. Total Environ. 13 (1e2), 49e65, 1992.
the antibiotic resistant bacterial diversity and its correlation with physico- Piccolo, A., Pietramellara, G., Mbagwu, J.S.C., 1996. Effects of coal derived humic
chemical parameters. J. Clean. Prod. 236, 117715. substances on water retention and structural stability of Mediterranean soils.
Awasthi, M.K., Wang, Q., Awasthi, S.K., Wang, M., Chen, H., Ren, X., Zhao, J., Zhang, Z., Soil Use Manag. 12, 209e213.
2018. Influence of medical stone amendment on gaseous emissions, microbial Qi, Y., Hoadley, A.F.A., Chaffee, A.L., Garnier, G., 2011. Characterisation of lignite as an
biomass and abundance of ammonia oxidizing bacteria genes during biosolids industrial adsorbent. Fuel 90 (4), 1567e1574.
composting. Bioresour. Technol. 247, 970e979. Richard, T.L., Hamelers, H.V.M., Bert), Veeken, A., Silva, T., 2002. Moisture re-
Benito, M., Masaguer, A., Moliner, A., Arrigo, N., Palma, R.M., 2003. Chemical and lationships in composting processe. Compost Sci. Util. 10 (4), 286e302.
microbiological parameters for the characterization of the stability and matu- Saha, B.K., Rose, M.T., Wong, V., Cavagnaro, T.R., Patti, A.F., 2017. Hybrid brown coal-
rity of pruning waste compost. Biol. Fertil. Soils 37, 184e189. urea fertiliser reduces nitrogen loss compared to urea alone. Sci. Total Environ.
Boehm, H., 1994. Some aspects of the surface chemistry of carbon blacks and other 601e602, 1496e1504.
carbons. Carbon 32, 759e769. Satisha, G.C., Devarajan, L., 2007. Effect of amendments on windrow composting of
C
aceres, R., Malin sk, K., Marfa
, O., 2018. Nitrification within composting: a review. sugar industry pressmud. Waste Manag. 27, 1083e1091.
Waste Manag. 72, 119e137. Schaub-Szabo, S.M., Leonard, J.J., 1999. Characterizing the bulk density of compost.
Castaldi, P., Garau, G., Melis, P., 2008. Maturity assessment of compost from Compost Sci. Util. 7 (4), 15e24.
municipal solid waste through the study of enzyme activities and water-soluble Shen, J., Chen, D., Bai, M., Sun, J., Coates, T., Lam, S.K., Li, Y., 2016. Ammonia depo-
fractions. Waste Manag. 28 (3), 534e540. sition in the neighbourhood of an intensive cattle feedlot in Victoria, Australia.
Chen, D., Sun, J., Bai, M., Dassanyake, K.B., Denmead, O.T., Hill, J., 2015. A new cost- Sci. Rep. 6, 32793.

9
Y. Cao, M. Bai, B. Han et al. Journal of Cleaner Production 277 (2020) 122422

Shi, X.Z., Hu, H.W., He, J.Z., Chen, D.L., Suter, H.C., 2016. Effects of 3,4- composting. Microb. Ecol. 60, 807e815.
dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and Yang, Z.H., Xiao, Y., Zeng, G.M., Xu, Z.Y., Liu, Y.S., 2007. Comparison of methods for
community composition of soil ammonia oxidizers in three land uses. Biol. total community DNA extraction and purification from compost. Appl. Micro-
Fertil. Soils 52, 927e939. biol. Biotechnol. 74, 918e925.
Sun, J., Bai, M., Shen, J., Griffith, D.W.T., Denmead, O.T., Hill, J., Lam, S.K., Mosier, A.R., Yue, Z.R., Jiang, W., Wang, L., Gardner, S.D., Pittman, C.U., 1999. Surface character-
Chen, D., 2016a. Effects of lignite application on ammonia and nitrous oxide ization of electrochemically oxidized carbon fibers. Carbon 37, 1785e1796.
emissions from cattle pens. Sci. Total Environ. 565, 148e154. Zeng, G., Zhang, J., Chen, Y., Yu, Z., Yu, M., Li, H., Liu, Z., Chen, M., Lu, L., Hu, C., 2011.
Sun, Z.Y., Zhang, J., Zhong, X.Z., Tan, L., Tang, Y.Q., Kida, K., 2016b. Production of Relative contributions of archaea and bacteria to microbial ammonia oxidation
nitrate-rich compost from the solid fraction of dairy manure by a lab-scale differ under different conditions during agricultural waste composting. Bio-
composting system. Waste Manag. 51, 55e64. resour. Technol. 102, 9026e9032.
Wu, Y., Chen, Y., Shaaban, M., Zhu, D., Hu, C., Chen, Z., Wang, Y., 2019. Evaluation of Zhang, J., Lv, F., Luo, C., Shao, L., He, P., 2014. Humification characterization of bio-
microbial inoculants pretreatment in straw and manure co-composting process char and its potential as a composting amendment. J. Environ. Sci. 26, 390e397.
enhancement. J. Clean. Prod. 239, 118078. Zucconi, F., Pera, A., Forte, M., Bertoldi de, M., 1981. Evaluating toxicity of immature
Yamamoto, N., Otawa, K., Nakai, Y., 2010. Diversity and abundance of ammonia- compost. Biocycle 22, 54e57.
xxidizing bacteria and ammonia-oxidizing archaea during cattle manure

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