QuantStudio 3 and QuantStudio 5

Real-Time PCR Systems

Just the right everything
Data within your reach
Applied Biosystems™ QuantStudio™ 3 and 5 Real-Time PCR Systems allow you to
remotely monitor your runs, as well as easily access and securely share results
with colleagues anywhere, anytime with Connect, our cloud-based platform.
When your data are always within reach, the answers shaping the future of
science are never far away.

Interactivity Connectivity* Collaboration*

• Run and edit directly • Telemetry data monitoring • Quickly share data sets and
from touchscreen to proactively anticipate protocols online
maintenance needs
• Send large files securely
Accessibility*
• Calendars to schedule time around campus or around
• Access experiment runs
on shared instruments for the world
from any location, anytime,
the best utilization and return
with remote monitoring • Integrate and analyze
on investment
multiple data sets and data
• Wi-Fi–enabled connectivity
• Integration with scientific types into one project
• Utilize portable devices to analysis apps and peer * Internet access and Connect cloud-based account required.
quickly analyze data when collaboration tools
you need to
• Remote support from our
services team

• World-class data security

on an Amazon Web
Services™ platform

100

Obtain results you can trust—Detect differences in

target quantity as small as 1.5-fold in singleplex reactions 10

and obtain 10 logarithmic units of linear dynamic range

(Figure 1). 1
ΔRn

Helps save valuable time— 0.1

Applied Biosystems™ VeriFlex™ Blocks

with 3 or 6 independent temperature 0.01

zones provide flexibility to run multiple Cycle

1 101 102 103 104 105 106 107 108 109 1010
experiments simultaneously. Fast thermal cycling is also
available, enabling results in less than 30 minutes. Figure 1. Real-time PCR reproducibility. This plot shows results from
amplification of KAZ plasmid DNA in 10-fold dilutions using the 96-well
block. The data show highly reproducible results over 10 logarithmic units
of input template amount, illustrating the broad linear dynamic range of
the system.

2
Simple, intuitive interface—at your fingertips
The interactive touchscreen interface • Manipulate view to a particular Service and support plans designed
and simplified Applied Biosystems™ graph or data point (Figure 2B) for you
QuantStudio™ Design and Analysis Our extended warranty plans are
Software make it easy to get started • Option to pause a real-time PCR run designed to maximize instrument
and stay organized. on demand performance and help ensure availability
• Preoptimized protocol templates of critical systems with preventive
• Software can be accessed either via maintenance, proactive instrument
desktop or online allow quick selection of default
protocols for standard applications monitoring, remote diagnostic
• Easily identifiable icons guide you capabilities, and—should one of
through the workflow to set up runs • Locked workflow feature allows for your instruments require repair—fast
and analyze experiments experimental consistency in tightly response. Benefits include:
controlled environments
• Graphical interface allows easy • Guaranteed 2 business day
editing of experimental conditions response time*
and viewing of plate layout • Scheduled on-site planned
(Figure 2A) maintenance (PM)
A B • Parts, labor, and travel for repair

• Remote instrument diagnostics

• Priority access to remote

service engineer

To learn more about our services

and support solutions, go to
thermofisher.com/instrumentservices
Figure 2. Graphical interface. Software allows (A) easy editing of thermal cycling conditions
and viewing of plate layout, and (B) viewing of amplification plots and drilling down to a subset of
* Guaranteed response times vary by region.
sample wells.

Get started quickly—Instrument is factory-calibrated Establish standard operating procedures

for optical and thermal accuracy, quick installation, and and compliance with ease—Locked
immediate use. protocol templates, in-run quality control (QC)
feedback, and QC traceability of consumables
Skip the learning curve—Sign into EducationConnect with offer greater control of experimental data. Real-time
your Connect account for an online course on installing, data markup language (RDML) export is available for
operating, and maintaining your instrument. To begin the compatibility with MIQE guidelines.
course, go to thermofisher.com/quantstudio3-5training.

Maximize benchtop space—Compact instrument can

be configured as a stand-alone unit or with a computer
to fit most laboratory needs (instrument dimensions:
27 x 50 x 40 cm).

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Technical specifications

QuantStudio 3 Real-Time QuantStudio 5 Real-Time

PCR System PCR System
Sample capacity (wells) 96 96 or 384
96-well 0.1 mL block: 10–30 μL
0.1 mL block: 10–30 μL
Reaction volume 96-well 0.2 mL block: 10–100 μL
0.2 mL block: 10–100 μL
384-well block: 5–20 μL
Excitation source Bright white LED
96-well: 6 decoupled filters
Optical detection 4 coupled filters
384-well: 5 coupled filters
96-well: 450–680 nm/500–730 nm
Excitation/detection range 450–600 nm/500–640 nm
384-well: 450–650 nm/500–700 nm
96-well: up to 6 targets
Multiplexing Up to 4 targets
384-well: up to 5 targets
96-well 0.1 mL block: 9.0°C/sec
0.1 mL block: 9.0°C/sec
Maximum block ramp rate 96-well 0.2 mL block: 6.5°C/sec
0.2 mL block: 6.5°C/sec
384-well block: 6.0°C/sec
Average sample ramp rate 3.66°C/sec
Temperature uniformity 0.4°C
Temperature accuracy 0.25°C
FAM/SYBR Green, VIC/JOE/HEX/TET,
FAM™/SYBR™ Green, VIC™/JOE™/HEX™/
ABY/NED/TAMRA/Cy3, JUN, ROX/
Compatible dyes TET™, ABY™/NED™/TAMRA™/Cy®3,
Texas Red, Mustang Purple™, Cy®5/
JUN™, ROX™/Texas Red™
LIZ™, Cy®5.5
Security, auditing, and e-signature
No Yes, with no additional fees
(SAE) features

4
Flexible, powerful secondary analysis software
Applied Biosystems™ Analysis Modules are innovative cloud-based data analysis
applications that bring together multiple data sets in one convenient place and
render them in stunning data visualizations for enhanced analysis and insights
(Figures 3–5).

Figure 3. Absolute quantification module for gene expression

analysis. The module enables analysis of genes of interest with the use of
a standard curve. Additional flexibility is achieved by importing standard
curves from other experiments.

Figure 4. Relative quantification module for gene expression analysis.

With this module, you can customize groupings of data within projects
for a thorough comparison of data. The module also includes integrated
correlation, volcano, and cluster plot analysis, with the ability to drill down
to amplification plots.

Figure 5. Genotyping analysis module. This module expands on existing

Applied Biosystems™ TaqMan® Genotyper™ Software with improved visuals
and integrated traces of allelic discrimination plots. The module allows for
thorough quality control of single-nucleotide polymorphism (SNP) assays
to accurately reflect true signals vs. background noise.

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High-quality data for a variety of applications
Utilizing proven Applied Biosystems™ OptiFlex™ technology and VeriFlex™ Blocks,
QuantStudio 3 and 5 systems offer optimal data accuracy and sensitivity
(Figure 6). Applications include analysis of gene expression, microRNAs and
noncoding RNAs, SNPs, copy number variation, somatic mutations, drug
metabolism enzymes, and protein expression.

A 10
B 40.0
37.5
35.0
32.5
30.0
1 27.5
25.0
22.5
ΔRn

Ct
20.0
17.5
0.1 15.0
12.5
10.0
7.5
5.0
0.01
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle Quantity
1,000 1,500 3,000 4,500 6,667 10,000 NTC 1 101 102 103 104 105 106 107 108 109 1010

Figure 6. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences.
(A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with the Applied Biosystems™ PE2 TaqMan® Assay under fast run conditions using
Applied Biosystems™ TaqMan® Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059);
3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard
curve generated from the Ct values.

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Assay flexibility to support your application
The QuantStudio 3 and 5 systems support probe-based assays as well as
intercalating dyes (Figures 7–9). TaqMan probe-based assays, developed with
powerful algorithms and optimized master mixes, enable outstanding specificity
and sensitivity. Applied Biosystems™ SYBR™ Green chemistry is an economical
alternative for target identification or initial qualification of assays.

6900000

5900000
Derivative reporter (–Rn´)

4900000

3900000 Figure 7. Melt curve analysis using the online version of the software.
In this experiment, 96 replicates of human genomic DNA were amplified
2900000
using Applied Biosystems™ SYBR™ Select Master Mix with primers for
1900000 the RNase P gene, followed by a dissociation step. The reactions were
performed under fast run conditions, showing Ct uniformity with a mean of
900000 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak
with a melting temperature (Tm) of 84.17°C (SD 0.07°C).

Temperature (°C)

10

1
Figure 8. Multiplex gene expression analysis of 4 targets. Whole-
plate amplification plots are shown of 96 replicates of cDNA made from
ΔRn

universal human RNA (UHR) amplified under fast run conditions using
0.1 Applied Biosystems™ TaqMan® Multiplex Master Mix with Mustang Purple
passive reference dye. Targets and labels: FZD1 labeled with FAM dye,
APOE labeled with VIC dye, CD44 labeled with ABY dye, GAPDH labeled
with JUN dye.
0.01

Cycle
CD44 APOE FZD1 GAPDH

3.3
2.8
2.3
Allele 2

1.8
Figure 9. Genotyping analysis that includes cluster plots with data
1.3 traces. An allelic discrimination plot is shown of 44 gDNA samples and
0.8 4 no-template controls (NTCs) genotyped using Applied Biosystems™
0.3 TaqMan® SNP Genotyping Assay C_29086771_20, with both PCR and allelic
discrimination performed on the QuantStudio 5 Real-Time PCR System. The
0.3 0.8 1.3 1.8 2.3
novel use of real-time PCR data to plot SNP cluster progress aids in calling
Allele 1
ambiguous samples and reduces run times by displaying the optimal number
Homozygous allele 1/allele 1 Homozygous allele 2/allele 2
of cycles necessary for maximum cluster separation.
Heterozygous allele 1/allele 2 Undetermined

For more information about TaqMan Assays and formats, go to thermofisher.com/taqman

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Ordering information
Instrument with
Instruments Cat. No. service—Cat. No.*
QuantStudio 3 system configurations
QuantStudio 3 Real-Time PCR System (96-well, 0.1 mL block)** A28136 A33777
QuantStudio 3 Real-Time PCR System (96-well, 0.2 mL block)** A28137 A33779
QuantStudio 5 system configurations
QuantStudio 5 Real-Time PCR System (96-well, 0.1 mL block)** A28138 A33619
QuantStudio 5 Real-Time PCR System (96-well, 0.2 mL block)** A28139 A33624
QuantStudio 5 Real-Time PCR System (384-well block)** A28140 A33628
* Extended warranty packages for the QuantStudio 3 and 5 Real-Time PCR Systems include the instrument, SmartStart™ Orientation, and a 1-year AB Assurance service plan with 1 planned maintenance (PM)
visit. Packages are not available in all countries. Contact your local sales representative for availability information.
** Does not include a computer. Additional Cat. No. are available that include a laptop or desktop computer.

Reagents Quantity Cat. No.

TaqMan Fast Advanced Master Mix 5 mL 4444557
TaqMan Fast Advanced Master Mix 50 mL 4444558
PowerUp SYBR Green Master Mix 5 mL A25742
PowerUp SYBR Green Master Mix 50 mL A25743
SuperScript IV VILO Master Mix 500 reactions 11756500
High-Capacity RNA-to-cDNA Kit 50 reactions 4387406
qPCR plastics Quantity Cat. No.
MicroAmp Optical 96-Well Reaction Plate 10 plates N8010560
MicroAmp EnduraPlate Optical 96-Well Clear Reaction Plates with Barcode 20 plates 4483354
MicroAmp Fast Optical 96-Well Reaction Plate, 0.1 mL 10 plates 4346907
MicroAmp EnduraPlate Optical 96-Well Fast Clear Reaction Plates with Barcode 20 plates 4483485
MicroAmp Optical 384-Well Reaction Plate with Barcode 50 plates 4309849
MicroAmp EnduraPlate Optical 384-Well Clear Reaction Plates with Barcode 20 plates 4483285
MicroAmp Optical Adhesive Film 100 covers 4311971
Instrument qualification (IQ), operational qualification (OQ),
and instrument performance verification (IPV) services† Quantity Cat. No.
IQ/OQ/IPV Service for QuantStudio 3 Real-Time PCR Systems, 96-well, 0.1 mL block 1 service A28481
IQ/OQ/IPV Service for QuantStudio 3 Real-Time PCR Systems, 96-well, 0.2 mL block 1 service A28480
IQ/OQ/IPV Service for QuantStudio 5 Real-Time PCR Systems, 96-well, 0.1 mL block 1 service A28483
IQ/OQ/IPV Service for QuantStudio 5 Real-Time PCR Systems, 96-well, 0.2 mL block 1 service A28482
IQ/OQ/IPV Service for QuantStudio 5 Real-Time PCR Systems, 384-well block 1 service A28484
†
OQ/IPV service is not available in all countries. Contact your local sales representative for availability information.

Find out more at thermofisher.com/quantstudio3-5

For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks
are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche
Molecular Systems, Inc., used under permission and license. Cy is a registered trademark of GE Healthcare. Amazon Web Services is a
trademark of Amazon Technologies, Inc. COL33293 1219