Title: Mastering the Challenge: Crafting Your Dissertation on Embryonic Stem Cells

Embarking on the journey of writing a dissertation is akin to navigating through uncharted waters.
It's a formidable task that demands unwavering dedication, meticulous research, and unparalleled
expertise. And when it comes to delving into the intricate realm of embryonic stem cells, the
challenge only intensifies.

Dissertations on embryonic stem cells delve into one of the most groundbreaking and controversial
fields in modern science. They require a comprehensive understanding of not only the biological
intricacies of stem cells but also the ethical and societal implications that accompany their research
and application.

The process of crafting a dissertation on embryonic stem cells is laden with obstacles. From
formulating a research question that strikes the perfect balance between innovation and feasibility to
conducting exhaustive literature reviews to establish the groundwork for your study, every step is
fraught with complexities.

Furthermore, the experimental nature of stem cell research adds another layer of difficulty. Designing
robust methodologies, navigating ethical considerations, and interpreting often ambiguous results
necessitate a level of expertise that few possess.

Amidst these challenges, it's natural for aspiring researchers to seek assistance. However, not all
avenues for help are created equal. In such a critical endeavor, entrusting your dissertation to just
anyone is a risk not worth taking.

That's where ⇒ HelpWriting.net ⇔ comes in. With a team of seasoned experts well-versed in the
nuances of stem cell research, we provide unparalleled support tailored to your specific needs.
Whether you require assistance with literature review, methodology design, data analysis, or
manuscript preparation, our dedicated professionals are here to guide you every step of the way.

By choosing ⇒ HelpWriting.net ⇔, you're not just outsourcing a task – you're investing in the
success of your dissertation. Our commitment to excellence ensures that your research is not only
academically rigorous but also ethically sound, setting you on the path to academic achievement and
groundbreaking discoveries.

Don't let the daunting nature of dissertation writing on embryonic stem cells deter you from pursuing
your passion. With the right support system in place, you can conquer any challenge that comes your
way. Trust ⇒ HelpWriting.net ⇔ to be your partner in academic success, and let your research
shine bright in the realm of stem cell science.
During pregnancy, they develop into all of the cells and tissues that form the fetus and the newborn
it grows into. Then, those mature cells replace tissue that is damaged by a disease or injury. Next,
each response will be listed per interview question. This enzyme is a reverse transcriptase that is able
to synthesize the ends of chromosomes, preventing senescence. Most often this can be a failure of
the donor cells to successfully graft, or a loss of these donor cells following a period of successful
engraftment. Ad How we are working to go beyond managing symptoms to addressing the true
cause of diseases. Once during a noon intercession, I performed my ablutions to pray and I.
Nonetheless, it is relevant to remark that the recent iPSC applications in toxicology and drug research
provide new alternatives to the standard routine tests performed by industry and offer new chemical
safety assessment strategies ( Laustriat et al., 2010; Trosko and Chang, 2010 ). Newbury Park, CA.:
Sage. Merriam, S. (2009).Qualitative research: a guide to design and implementation. Once created,
the blastocyst embryos can be preserved indefinitely under laboratory conditions. New York, NY:
Guilford Press. Yin, R. (1994). Case Study Research: Design and Methods, Sage, Beverly Hills, CA.
Yin, R. (2009). Case Study Research: Design and Methods. Bearing this in mind, scientists also do
not believe that this should derail stem cell therapies considering that there are DNA tests to check if
stem cells will turn out to be problematic or not. We have intelligent experts and perfect platform to
ensure our performance in stem cell research. Afterward, each well is assessed for the cell number
and differentiation to cardiomyocytes (using quantitative in-cell Western analysis for myosin heavy
chain protein normalized with the cell number). Therefore, the basis of this research violates
women’s reproductive autonomy, thus violating the Principle of Beneficence. When kept under the
right conditions, stem cells will grow and divide indefinitely, without becoming differentiated.
Scientists are prohibited from studying human embryos in their labs beyond 14 days of
development. Stem Cell Information from the National Institute of Health, U. He found a way to
take ordinary skin cells and turn them into cells that behave just like embryonic stem cells, but
without destroying an embryo. The most salient characteristic of this reality o. Stem cells will be
active when there are dead cells present and it has the ability to replace dead cells in our body.
Summary This chapter presented the methodology used in this study. Patients eligible for
Haematopoietic Cell Transplantation (HCT) first undergo an induction therapy, which can last
upwards of 4 months. Schematic Representation Of How Embryonic Stem Cells Are Derived
Download Scientific Diagram Liver cancer stemness refers to the stem cell-like phenotype of
hepatocarcinoma cells and is closely related to a high degree of tumour malignancy.. Human
embryonic stem cells hESCs are derived from the inner cell mass ICM of embryonic blastocysts. In
light of these two opposing views, should embryonic stem cells be used in research. Greed has made
many people wild in history and still does. When the cell differentiates, it assumes the structure,
function, and biochemistry of that cell type. Embryonic stem cells ESCs are characterized by a dual
capacity self. European Centre for the Validation of Alternative Methods. Data will be collected
using a semi-structured, open-ended interview questionnaire.
Finally, as the cells continue to become more specialized, they are said to be multipotent. To
complicate matters, as each technological innovation arrives, stem-cell scientists will have to re-
define the start of life as many times as there are new technological developments, an exhausting
and never-ending process that would ultimately lead us back to moment of fertilization. Creswell, J.
W. (1998). Qualitative inquiry and research design: Choosing among five traditions. To conclude,
stem cells possess the vast potential to cure many medical conditions due to which this field of
research is gaining momentum in contemporary times; however, protests against Embryonic stem cell
research are hindering further research and identification of breakthrough findings that can benefit
mankind. Also, note that stem cells exist not only in embryos, but also in adults—we have stem cells
that give rise to skin, blood, and the epithelial lining of the digestive tract, among others. Yet, every
human ought to be regarded as autonomous with rights that are equal to every other human being.
This is 100% legal. You may not submit downloaded papers as your own, that is cheating. Also you.
In the treatment of ES cells with trichostatin A (TSA), a specific HDAC inhibitor has been reported
which not only induces the acetylation of both GATA4 and histones, but facilitates their
differentiation into cardiomyocytes ( Kawamura et al., 2005 ). Embryonic stem cells ES cells are
derived from the inner cell mass of the blastocyst. This type of stem cell is known as embryonic stem
cell. In traditional human pregnancy, the blastocyst stage occurs before the embryo reaches — and
implants within — the mother’s uterine wall. A cell that has become a special type may be able to
divide to make more of the same type, but cannot make different types. Telomeres are difficult to
replicate, however, so they shorten during each round of DNA synthesis. Dependability
Dependability accounts for results being subject to changes in the conditions and design of the study
through the use of accurate documentation during the data collection process (Creswell, 1998).
Bearing this in mind, scientists also do not believe that this should derail stem cell therapies
considering that there are DNA tests to check if stem cells will turn out to be problematic or not.
Example of a conclusion Here is a sample conclusion: Stem cell research has great potential in
understanding illnesses and treating incurable diseases apart from ending human and animal
experimentation. A good thesis is based on thoughtful research and not a simple rewriting of facts.
Source: (NIH, n.d.) The use of HSC therapies is associated with a number of general complications
that must be appropriately planned for, considered and balanced. In all cases, differences in
expression as regards the control were statistically significant for at least p Romero, 2010 ). During
the reign of President Bush, federal funding of human Embryonic stem cell research stood banned
since the administration opposed the practice. What lineages can a cell of each type differentiate
into. As HSCs can be found in both bone marrow and peripheral blood, they are readily accessible.
As discussed previously, surrounding tissues induce a developing cell to become a particular cell
type via inducers; the term inducer may also refer to the cell secreting the signal. During this process,
it will become apparent that the participants' responses are collapsing around common areas related
to the interview questions. The in vitro- in vivo evaluation, including the selection of a practical
battery of cell tests for prediction of acute lethal blood concentrations in humans. This was probably
due to the fact that only two endpoints were selected for the biostatistical assessment in the ESC
assays, which seems insufficient. As the government cut funding for embryonic stem cell research
initiatives, scientists must rely on private funding to continue their studies (Stake, 2010). A bipartisan
Congress voted to ease federal restrictions, legislation which Bush vetoed. Since an embryo is
regarded as a human being with morally obligated rights, the Principle of Beneficence is violated,
and the autonomy and welfare of the embryo is not respected due to the destruction of an embryo in
stem cell research. As this results in a reduction in all types of blood cells, Erythrocyte and platelet
transfusions can be provided if required.
Indeed, ECVAM has recommended the inclusion of molecular endpoints in the EST with a view to
improving its performance ( Spielman et al., 2006 ). A great deal of work has been done to follow
this recommendation in an attempt to introduce gene expression as biomarkers of differentiation.
With knowledge of embryonic stem cells having higher complications than the viable adult stem cells
continued use of embryonic stem cells violates the Principle of Beneficence not only for the embryos
but for the health and safety of the patients treated with stem cells. Personal approach to every
customer is our firm guarantee. Undifferentiated cells capable of cell division, can proliferate into the
millions unlike other cells. 2. Can be induced to become a specific type of cell. Having concluded
that a human embryo has the moral equivalent of a fully-fledged human being, this field of research
clearly violates the amiable rights of personhood, and in doing so discriminates against pre-born
persons. The cytotoxic effects on D3 and 3T3 cells were estimated by determining those
concentrations of the test substance at which proliferation was inhibited by 50% after 10 days of
exposure, yielding IC 50 D3 and IC 50 3T3, respectively. They can also turn into different kinds of
cells, known as differentiation. Opposition to the federal funding of embryonic stem cell research
can only be justified by an appeal to unreasonable and arbitrary moral standards based not on logic,
reason, or concern for human well-being, but rather on the dictates of outdated and dangerous
religious beliefs. Stem Cells Embryonic Stem Cells Side Track Adult Stem Cells Treatments.
Unipotent cells are those that only differentiate into one cell type, usually to maintain a particular cell
line. For instance, bone cells can originate from osteocyte cells, or liver tissues can come from
hepatocytes. This section briefs you about company addresses, contact details, products, and regional
presence of companies who are purchasing or are likely to purchase. When the cell differentiates, it
assumes the structure, function, and biochemistry of that cell type. Embryonic Stem cells Derived
from embryos the develop from fertilized human embryos. There are ethical boundaries that are
crossed when natural processes of life are altered or manipulated. Those breakthroughs not only
meant higher success rates for hopeful couples, they also created more viable embryos from which
stem cells could be extracted. Under right conditions, can become any cell type in our body. Much
later, they isolated human embryonic stem cells in 1998. It may lead to more questions than new
answers and the ethical risks are substantial. Cellular therapies in acute lymphoblastic leukemia. For
instance, bone cells can originate from osteocyte cells, or liver tissues can come from hepatocytes. Dr.
Eckman asserts that “every human being has a right to be protected from discrimination.” Thus,
every human, and by extension every embryo, has the right to life and should not be discriminated
against their for “developmental immaturity.” Therefore, the field of embryonic stem cell research
infringes upon the rights and moral status of human embryos. Content Alerts Brief introduction to
this section that descibes Open Access especially from an IntechOpen perspective How it Works
Manage preferences Contact Want to get in touch. The cells get harvested from the ICM after
scientists penetrate the outer layer. Embryonic stem cell research continues to provoke serious
controversies. Our pieces cover all aspects of bioethics from clinical to philosophical, addressing
timeless ethical dilemmas as well as current challenges. This newly revised high throughput EST
allowed the analysis of a larger number of substances, less manual work, yet it yielded data
comparable to those obtained with the conventional EST. The adverse effects are not balanced by a
clear increase if efficacy over AutoHCT and chemotherapy (Barlogie et al., 2006). Furthermore, the
ineligibility of many patients based on age or co-morbidities limits the scope of AlloHCT as the gold-
standard MM treatment protocol. Participants will also be informed that they would complete a brief
demographic questionnaire at the beginning of the interview session. The methods of data collection
were described in detail, including how the study population was recruited, and the information
contained in the consent form.