RT-2100C

Microplate Reader
Service Manual

Copyright: Rayto Life and Analytical Sciences Co.,Ltd.

Address: C&D/4F, 7th Xinghua Industrial Bldg, Nanhai Rd, Nanshan, Shenzhen 518067, P.R.
China

Version:1.4e

25
 CONTENT
Note of this manual .................................................................................................................................. 2
General Description ................................................................................................................................. 3
1.1. Foreword .................................................................................................................................. 3
1.2. Technical parameters ............................................................................................................... 4
1.3. Diagram of function .................................................................................................................. 5
1.4. Function description of each part ............................................................................................. 6
1.4.1. Function description of power supply board ................................................................. 6
1.4.2. Function description of mainboard ................................................................................ 6
1.4.3. Function description of front-end board ........................................................................ 6
1.4.4. Function description of motor driver board ................................................................... 6
1.4.5. Function description of LCD transition board................................................................ 6
1.4.6. Function description of LCD screen .............................................................................. 6
1.5. Optic system............................................................................................................................. 7
1.6. General views of RT-2100C ..................................................................................................... 7
1.6.1. RT-2100C front view...................................................................................................... 7
1.6.2. RT-2100C rear view ...................................................................................................... 8
2. Installation and primary operation .................................................................................................. 9
2.1. Instrument unsealing ................................................................................................................ 9
2.2. Environmental requirements .................................................................................................... 9
2.3. Pinter installation .................................................................................................................... 10
3. Daily maintenance and spare parts replacement ........................................................................ 11
3.1. Maintenance of the instrument................................................................................................11
3.1.1. Instrument cleaning ......................................................................................................11
3.1.2. Maintenance of LCD touch-screen ..............................................................................11
3.2. How to disassemble the instrument ........................................................................................11
3.3. Replacement of spare parts ................................................................................................... 12
3.3.1. Replacement of fuses ................................................................................................. 12
3.3.2. Replacement of power switch ..................................................................................... 13
3.3.3. Replacement of Lamp ................................................................................................. 13
3.3.4. Replacement of plate carrier ....................................................................................... 15
3.3.5. Replacement of the belt. ............................................................................................. 16
3.3.6. Replacement of filters ................................................................................................. 17
3.3.7. Replacement of touch panel/LCD screen ................................................................... 19
3.3.8. Replacement of LCD transition board ......................................................................... 20
3.3.9. Replacement of the front board .................................................................................. 20
3.3.10. Replacement of main board ........................................................................................ 21
3.3.11. Replacement of optical fibre ....................................................................................... 22
3.3.12. Replacement of driver board ...................................................................................... 25
3.3.13. Replacement of power supply .................................................................................... 26
4. Trouble shooting ............................................................................................................................. 27
Appendix I Data transfer protocol of RT-2100C .................................................................................. 31

1
Note of this manual

Note: for uncorrectable errors or repeated occurrence of an error during usage,

please contact the dealer.

Note: this manual may contain other declarations of proprietary rights and
copyright information that must be observed. This manual may contain
inaccurate technical information or misprints.

This manual is subject to change or updating without separate notice. Rayto

Incorporation can improve and/or modify the products and/or programs
described in this manual at any time without separate notice.

2
General Description

1.1. Foreword

1) RT-2100C is a microprocessor –controlled, general purpose photometer system designed to read

and calculate the result of assays, including contagion, tumorous mark, hemopathy,
dyshormonism, which are read in microplate.
2) Touch panel and pen brings you convenience in operation.
3) 100 tests can be programmed.
4) Multiple calculations：
⚫ Absorbance mode (ABS)
⚫ Cut-Off mode
⚫ Single standard mode
⚫ Point to point mode
⚫ % Absorbance Multi-Point Mode
⚫ Linear regression mode
⚫ Exponent regression mode
⚫ Logarithm regression mode
⚫ Power regression mode
5) Visual allocation of microplate，you can set blank, control, sample ,standard in any place，and
perform 12 different tests in one 96 microplate.
6) Test time ＜5S/plate,and mix plate before test
7) Maximum 1000 patient data and 10000 sample records can be saved.
8) Comprehensive report ，support common printers.
9) Information manage function：Department database, operator database, system log database.

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1.2. Technical parameters

Weight: 7.5kg
Overall dimensions: 460mm（L）×330mm（W）×190mm（H）
Power: a.c.110V~220V，50/60Hz
Fuses: T3.15AL250V
Work Environment: temperature 10℃～40℃；Humidity ≤85%
Store Environment: -10℃～40℃；Humidity ≤85%
Lamp: Halogen tungsten lamp
Standard 405，450，492，630nm，(substitute filters from 340-700nm
Wavelength: available on special order)
Accuracy: 0-2.0A：±1.0% or ±0.007A
Precision: 0-2.0A：±0.5% or ±0.005A
Linearity: ±2.0% or±0.007A
Reading speed: Continuous mode ＜5s，step by step mode＜15s
Warm up time: 1 minute
Interface: RS-232C serial interface, Interface for USB, SD card and
LAN
Display: 5.7″LCD display（320×240 discernibility,256 gray scale）
Input: Touch panel and pen, external mouse（Optional）

4
1.3. Diagram of function

Figure 1-1 Diagram of function

5
1.4. Function description of each part

1.4.1. Function description of power supply board

The power of RT-2100C system is provided by switching power supply, which outputs 6 channels
of AC electric power.
JP1 outputs 5V, for the mainboard
JP2 outputs 5V, 15V and –15V, for the front board
JP3 outputs 12V, for the fan
JP4 outputs 5V, 12V and 24V for motor driver board
JP5 outputs 6.5V for Lamp
JP6 outputs 5V for printer driver board

1.4.2. Function description of mainboard

Mainboard is the platform of RT-2100C system, which provides the running environment for
system software and application software;
It provides the interface to connect front board, and controls the front board and data acquisition
procedure;
It provides the I/O interfaces for the system, supports one RS-232 interface, 2 USB interface, 1
LAN interface and 1 SD interface.

1.4.3. Function description of front-end board

The front board converts the instructions from the mainboard into driving signals and locating
signals, to control the filter wheel’s motor and control the predetermined-position attainment signal
and reset signal for the plate carrier. It also converts the photoelectric signal that the photocell
received into digital signal and returns it to the mainboard.

1.4.4. Function description of motor driver board

The motor board receives the control signal from front-end board, providing voltage and control
signals for filter wheel motor and plate carrier motor.

1.4.5. Function description of LCD transition board

LCD transition board controls the displaying of LCD. It provides high voltage for LCD screen
receives the signal from the touch panel and returns it to the mainboard.

1.4.6. Function description of LCD screen

The LCD screen displays and the touch panel receive the signal by external touches.

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1.5. Optic system

A/D transfer board

Optical diode

Display Secondary lens

CPU Signal amplifier
Print
Microplate

Filter wheel
Reference path Heat absorb board and lens

Membran
Fiber
e

board

Lamp

Diagram 1-3 Optic system of RT-2100C

1.6. General views of RT-2100C

1.6.1. RT-2100C front view

①

③
②

① power pilot lamp： It light when opens the instrument

② touch panel & LCD：displaying by LCD and you can operate by touch panel.
③ Plastic cover
④ Plate carrier：Microplate in plate carrier

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1.6.2. RT-2100C rear view

⑦ ④ ⑤ ⑥

② ③ ⑧
LINE PRINTER RS232

POWER ACINPUT
ONO
/ FF

①②③④ ⑤ ⑥

① Power switch
② Power socket
③ Fuses
④ Interface for SD Card
⑤ Interface for USB
⑥ Interface for LAN
⑦ RS-232 interface
⑧ Brightness adjuster (to adjust the brightness of displaying)

8
2. Installation and primary operation

2.1. Instrument unsealing

Unseal the packing of the instrument and remove the material used for conveyance. Retain the
packing box and packing material for the purpose of re-packing the instrument in future.
1） Take the instrument out of the box.
2） Remove the packing material and take the instrument out of the plastic packing bag.
3） Check the contents of the packing box for the following:
⚫ RT-2100C main system
⚫ user manual
⚫ Packing list
⚫ guarantee card
⚫ Accessories: power line, RS-232 serial cable, touch pen and spare fuses.

Note: if any part is found missing or damaged or not conform to the encasement
checklist, please contact the dealer.

2.2. Environmental requirements

Find a location in you working place that is not directly exposed to the sun. The working table
selected shall have a smooth surface as well as an enough space to put the RT-2100C. The front edge
of the instrument shall be close to the edge of the working table. Avoid much vibration on the working
table (e.g. a centrifugal machine is put on the table).

Note: the ambient temperature of the instrument shall be 10 ℃ -40 ℃ , and the
humidity shall be≤85％.

In order to ensure the normal working of the instrument, it must not be put in the following places:
⚫ Where there is a wide temperature range;
⚫ Where it is too hot or too cold;
⚫ Where there is a lot of dust;
⚫ Close to the electromagnetic equipment that generates magnetic field;
⚫ AC110V - AC220V
⚫ 50 / 60Hz
⚫ 120VA

9
Note:
⚫ AC mains must have a good earthing (zero-ground voltage＜5V＝.
⚫ AC power supply must be stable and the instrument must not share the AC
mains with large-power electrical appliances.
⚫ Must clutch at the plug itself, not at the power cord, to disconnect the power
cord.

If fumes, peculiar smells or strange sounds are found given forth from the instrument, please cut
off the electricity supply immediately and contact the dealer.

2.3. Pinter installation

1) Make sure that the printer and instrument have been turned off.
2) Connect one end of the USB printer cable to the USB interface of the printer.
3) Connect the other end of the USB printer cable to the USB Interface of instrument.
4) Connect the printer to power supply by the power line for the printer.

Note：To make the printer work properly, you must turn on the instrument before
you turn on the printer.
When you are inserting or pulling out the printer cable, you must make sure
that the printer has been turned off. Or it may cause damage to the
instrument and make it work abnormally.

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3. Daily maintenance and spare parts replacement

3.1. Maintenance of the instrument

RT-2100C is a precision instrument for clinic analysis, and in order to keep the instrument in a
good condition, daily maintenance is a must. The maintenance of RT-2100C is very simple, but
one must do it with care.

3.1.1. Instrument cleaning

⚫ Keep the instrument’s working environment clean.
⚫ Use neutral detergent and wet cloth to clean the surface of the instrument.
⚫ Please clean the LCD with soft cloth.
⚫ Clean the moveable working table with neutral detergent and wet cloth.

Note: please keep this instrument away from any organic solvent, grease or
corrosive substance.

3.1.2. Maintenance of LCD touch-screen

⚫ Please use the machines attached special-purpose pen for operation.
⚫ Never touch the screen surface with sharp-pointed or high-hardness objects (such as metal,
glass and etc), so as not to cause damage.
⚫ If there is a great deviation of the position the arrow points at from the position you point at, the
touch-screen need recalibration.

3.2. How to disassemble the instrument

Steps of disassemble
Steps of disassembly:
1） Fix the plate cover of the microplate reader with adhesive paper.
2） Switch off the instrument and the printer and disconnect the power cord.
3） Remove the printer cable.
4） Remove the screws used to fix the top cover with a cross screwdriver, as shown in figure 3-1.
5） Lift the top cover carefully and you can see the inside of the instrument.
6） Remove the corresponding parts according to your requirement.

Figure 3-1

11
 Figure 3-2

3.3. Replacement of spare parts

Note: you must observe carefully the positions and wiring of the original parts
before replacing them, so as to restore the instrument.

3.3.1. Replacement of fuses

1、 As shown in figure 3-3, use a screwdriver to remove the fuse from fuse box. Push the fuse
socket in and turn it anticlockwise, then you can take out the fuse socket, as shown in figure
3-4.

Fuse box

Figure 3-3

Figure 3-4

12
 2、After taking out the fuse socket, as shown in figure 3-5, take the fuse out form the socket and
replace a new one. Install the fuse socket into the fuse box again. Use a screwdriver to push
the fuse socket in and turn it clockwise.

Fuse

Fuse socket

Figure 3-5
3.3.2. Replacement of power switch
1、 Switch off the instrument, remove the screw which fixed the top cover and remove the
cover

Figure 3-6
2、 Press out the spring piece ④ at the two sides of switch socket, take out the whole switch
socket, the structure of power switch is shown as figure 3-6
3、 Take off the wires from the switch, change a new switch and connect the wires as original,
then re-install the switch

Note：Please pay attention to the original position of wires, they must be correctly
connected. You’d better connect the wire to new switch as soon as you
take it form the old switch.

3.3.3. Replacement of Lamp

Note: You must wait for the lamp to cool down, then you can replace it.

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1、 Lift the top cover of instrument, you can see the lamp, as shown in figure 3-7, the lamp is
shown as figure 3-8.

Lamp

Lamp socket

Figure 3-7

Figure 3-8
2、 As shown in figure 3-9, remove the screw and spring piece which fix the lamp, press the lamp
down slightly, take off the lamp form lamp socket, disconnect the wires on lamp, replace a new
lam and connect the wires again.
3、 The installation of lamp is shown in figure 3-10, press down the lamp slightly, push it into the
lamp socket and make it locked, then fix the lamp by screw and spring piece as original. At
last you shall check if the lamp has been fixed tightly. If not, you must take off the lamp again;
adjust the spring piece to make sure that it has enough power to fix the lamp.

Spring piece

Figure 3-9

14
 Figure 3-10
3.3.4. Replacement of plate carrier
1、Lift the top cover of instrument, remove the black shield box on the front board.
2、Remove the fixing bracket at right side of plate carrier, take off the two metal rails.

Fixing bracket

Figure 3-11

Plate carrier

Metal rail

Figure 3-12
3、As shown in figure 3-12, loosen the belt on the plate carrier, take it off from the motor at left side
of plate carrier, then upturn the plate carrier.

15
 Figure 3-12
4、Remove the two screws, you can take off the plate carrier. Replace a new one, re-install it as
original, fix the screws.

Gear

Figure 3-14
5、Upturn the plate carrier, install the belt to the gear on the plate carrier motor at left side, as
shown in figure 3-14.
6、Install the metal rails and fixing bracket, adjust the belt to make the plate carrier moves
smoothly, install the shield box and top cover of instrument.
3.3.5. Replacement of the belt.
1、Lift the top cover of instrument, remove the shield box on front board.
2、Remove the fixing bracket at right side of plate carrier, take out the two metal rails.

Plate carrier

Metal rail
Fixing bracket

(a) (b)
Figure 3-15

16
 3、As shown in figure 3-16 (a), loosen the belt on plate carrier, take it off from the motor at left side
of plate carrier, then upturn the plate carrier, as 3-16 (b) .

(a) (b)
Figure 3-16
4、 Remove the two screws shown in figure 3-16 (b), you can take off the plate carrier.

Belt

(a) (b)
Figure 3-17
5、Take off the old belt and replace a new one. The replacing procedure is shown as figure 3-17
(b), here please note that the side with tooth should face outside. In 3-18 (a) , please note
that the side with tooth should face inside.

Gear

(a) (b)
Figure 3-18
6、Install the plate carrier then upturn it, as shown in figure 3-18 (b), install the belt to the gear on
the plate carrier motor.
7、Install the metal rail and fixing bracket, adjust the belt to make the plate carrier move smoothly,
then install the shield box and top cover, restore the instrument.

3.3.6. Replacement of filters

1、Take off the label, you can see the hole of screw, as shown in figure 3-19.

17
 Figure 3-19
2、Use hex wrench M3 to loosen the two fixing screws, as shown in figure 3-20.

Figure 3-20
3、Take out the convergent mirror assembly, as shown in Figure 3-21 (a)

Convergent mirror Optic coupler bracket

(a) (b)
Figure 3-21

4、 Loosen the two M3 cross screws which fix the optical coupler bracket, please not that you
don’t need to take them off, as shown in figure 3-21 (b).
5、 Move away the optic coupler, take out the filter wheel, as shown in figure 3-22 (a)

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 450nm
492nm
405nm
Filter wheel

630nm

Gap

Black position

(a) (b)
Figure 3-22

6、 Replace filters according to wavelength as shown in figure 3-22 (b), please note that you can
use the gap to distinguish the hole for different filters.
7、 After changing the filters, re-install the filter wheel. Please note that the upside surface
between the optic couplers should align at the side board surface of the optical path, as
shown in figure 3-23.

Side board of optical

path
The two surface should
be align at

Figure 3-23
3.3.7. Replacement of touch panel/LCD screen
1、 Lift the top cover of instrument, remove the fixing screw on the main board, take out the main
board, as shown in figure 3-24 (a).

(a) (b)
Figure 3-24

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 2、 Remove the two data cable at right side of LCD form the transition board by forceps，as shown
in figure 3-24 (b).
3、 Remove the four screws which fix the LCD, take out the LCD and touch panel, as shown in
figure 3-25 (a) and (b).

(a) (b)
Figure 3-25
4、 Replace a new touch panel of the same model, restore the instrument.
Note: please pay attention to the sides of touch panel while installation, the rough side of the
touch panel is the touch side, which should be towards outside.

3.3.8. Replacement of LCD transition board

1、 Life the top cover of instrument, you can see the LCD transition board at the right side of LCD,
as shown in figure 3-26 (a).
Pull this socket to left
slightly; you can take out
Connect to LCD the LCD cable.

Connect to
touch panel

(a) (b)
Figure 3-26
2、 Remove all the cables on LCD transition board (including LCD cable and touch panel cable),
remove the 4 screws which fix the LCD Transition board, you can take out the board.
3、 Replace a new LCD Transition board, connect the LCD cable and touch panel cable, connect
all the wires, fix the four screws, install the shield box and top cover of instrument.
3.3.9. Replacement of the front board
1、 Lift the top cover of instrument, remove the shield box on the front board, you can see the
front board, as shown in figure 3-27.

20
 Figure 3-27
2、 Remove all the wires on the front board, remove the two screws as shown in above figure, you
can take out the front board.
3、 Replace a new front board, fix the screws, connect all the wires and make them in good order.
Please note that the Hoare unit must be installed above the groove of the worktable for front
board. The deviation can not be too big or there will be an error of “No positioning signal for
plate carrier”.

Hoare unit
Hoare unit Groove
(a) (b)
Figure 3-28
4、 Install the shield box and top cover of instrument.

3.3.10. Replacement of main board

1、 Lift the top cover of instrument; you can see one big board and one small board at the bottom
of instrument. The big one is the main board, as shown in figure 3-29.

21
 Figure 3-29
2、 Remove the screws which fix the main board, remove the cables connected to main board,
connect them to a new board.
3、 Install the main board, fix the screws and restore the instrument.

3.3.11. Replacement of optical fibre

1、 Remove the lamp cable to prevent lamp from broken by misoperation, as shown in figure
3-30.

Lamp cable

Figure 3-30
2、 Remove the shield box, remove the two screws which fix the platform by hex wrench M4, as
shown in figure 3-31.

Platform

Figure 3-31
3、 Remove the two screws which fix the front board by cross screwdriver M3, as figure 3-32.

22
 Figure 3-32
4、 Use a cross screwdriver M3 to loosen the screws which fix the optical fiber head, please note
that you do not need to take the screw out. Then fix the screw in the middle by hex wrench M3,
as shown in figure 3-33.

Figure 3-33
5、 Use a hex wrench M4 to loosen the two screws which fix the optical path, please note that you
don’t need to take them out, as shown in figure 3-34.

Figure 3-34
6、 Use a cross screwdriver M3 to remove the two screws which fix the optical fiber bracket, as
shown in figure 3-35.

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Optical fiber bracket

Figure 3-35
7、 Lift the platform; take out the optical fiber assembly, as shown in figure 3-36.

Optical fiber assembly

Figure 3-36
8、 The optical fiber is as figure 3-38; Remove the four screws which fix the optical fiber, replace a
new one, as shown in 3-39.

Figure 3-38

Figure 3-39

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 9. Replace a new optical fiber with the same model, install it carefully as original, fix the optical
fiber and its head, and restore the instrument.
10. Turn on the instrument, enter the backdoor procedure, turn on the lamp, turn the filter to
492nm.
11. Find a white thin paper; put it at the eight light points to watch the faculas, as shown in figure
3-40. The 8 faculas should be at the center position of the black hole as figure 3-40. If there is
deviation, you shall loosen the two screws which fix the optical fiber, fine tuning the position of
optical fiber, until the facula come to the center of the hole, then fix the screw again.

Figure 3-40
12. Install the platform, front board and shield box as original, install the top cover of instrument.
13. Enter the backdoor procedure of instrument, check the AD value of each channel, If the
deviation is too big, you have to adjust the position of optical fiber or the fiber is not good, you
must change a new fiber again.
14. Take calibration liquid of 2.0A to test the instrument at backdoor procedure, if it can not meet
the requirement, you shall fine tuning the position of the optic coupler on the optical fiber. We
do not suggest the engineer to replace the optical fiber at customer side.

3.3.12. Replacement of driver board

1、 Lift the top cover of instrument; remove the shield box on front board.
2、 Take off the lamp cable to prevent the lamp from broken by misoperation, as shown in figure
3-41.

Lamp cable

Figure 3-41

3、 Remove the two hex screws which fix the platform, lift the platform carefully (pay attention not
to pull the optical fiber), then you can see the motor driver board, as shown in figure 3-42.

25
 Optical fiber Motor driver board

Figure 3-42
4、 Take off all the wires on motor driver board and remember their positions, remove the four
screws which fix the board, take off the motor driver board, replace a new one, and install it as
original.
5、 Install the shield and top cover of instrument.

3.3.13. Replacement of power supply

1、 Lift the top cover of instrument; remove the shield box on front board.
2、 Take off the lamp cable to prevent the lamp from broken by misoperation, as shown in figure
3-43.

Lamp cable

Figure 3-43

3、 Remove the two hex screws which fix the platform, lift the platform carefully (pay attention not
to pull the optical fiber).
4、 Take off all the wires on the power supply and remember their positions, as shown in figure
3-44. Remove the screws which fix the power supply, then remove the screw which fix the
bottom of power supply, change a new one and restore the instrument.

26
 Figure 3-44

Note：
1、 There is some glue on the plugs, befor you take it out, you can use a
tool to remove the glue.
2、 If you are not familiar with the position of plugs on the board, you
shall remenber the cresponding positions carefully, so that you
will not make mistakes when you want to install the plug again.

4. Trouble shooting

Trouble Solution
The plate courier can not move The supporting frame in the middle of the plate courier is
smoothly on the board and rubs not properly installed. Reinstall the supporting frame.
against the optical grating board.
Report “492nm wavelength is too The lamp is aging. Replace the lamp.
weak” when start the machine. The light-filtering sheet is broken. Replace the
light-filtering sheet and adjust the frequency and
synchronization of the light-chopper wheel.

Report “shared ROM error” when Too short interval between the starts of the machine. It
start the machine. shall be above 3 minutes.
No ground wire or the ground wire is in poor contact.
Earthing the instrument properly.
The machine is started in the wrong order. You should
switch on the host computer first and then the printer.
The front-end board error. Add a 4.7K resistor to between
the 10th pin and the 18th pin of U11 on the front-end board.

27
Report “405nm wavelength is too The lamp fell off or is not properly installed. Reinstall the
weak” when start the machine. lamp.
The light-filtering sheet is broken. Replace the
light-filtering sheet.
The lamp is aging. Replace the lamp.
The optical fiber is damaged. Replace the optical fiber.
The photoelectric reaction board on the front-end board is
broken. Replace the photoelectric reaction board.

The clock is out of order and need The battery is electrically discharged. Replace the battery.
to be re-set up every time the
machine is started.
No display on the screen when The flat data cable that connects the displaying screen to
start the machine. the transfer board is loose or fell off. Re-plug it.
The ground wire of the transfer board fell off or is in poor
contact. Reconnect it.
The transfer board is broken or fell off. Replace it or solder
it.
The displaying screen is broken. Replace the displaying
screen.
The mainboard is broken. Replace the mainboard.

Report “shared ROM (RAM) error” No ground wire or the ground wire is in poor contact.
when start the machine, and then Earthing the instrument properly.
all the data stored in the machine The machine is started in the wrong order. You should
are lost. switch on the host computer first and then the printer.
The front-end board error. Add a 4.7K resistor to between
the 10th pin and the 18th pin of U11 on the front-end board.
The front-end board is broken. Replace the front-end
board.

The touch panel is inaccurately Re-locate the touch-screen.

located. The touch-screen is broken. Replace the touch-screen.
The Q1-Q6 triode on the mainboard is broken. Replace
the Q1-Q6 triode.

Feign positive in testing HbsAg. The sample is contaminated or incompetent. Re-make the
sample.
The range of negative contrast shall be set as 0.05-3.5.
The machine’s internal K values are altered. Re-set the K
values. Set all K1s as 1 and all K2s as 0.

28
Report the blank absorbency is The blank is contaminated. Use clean blank holes.
too high in testing Modify the blank from ≤0.05 to ≤0.1 in item setting.

The enzyme label plate is wedged The enzyme label plate is not properly placed or the plate
in the machine and can not be bar is incompetent. Open the machine housing and take
taken out, or wedged outside the the enzyme label plate out. Use the right one.
machine and can not be put in for
testing.
The screen suddenly becomes a The voltage is not stable. Connect it with a manostat.
blank screen, and after a while, it The flat data cable that connects the displaying screen to
restores normal displaying. the transfer board is loose. Re-plug it.

The clip hood of the plate courier Replace the plate courier.
is damaged.
The belt is broken. Replace the belt.

The printer can not print. The model of the printer is improperly set up. Re-set up
the model of the printer.
The printing cable fell off. Re-plug the printing cable.
The printer is broken. Replace the printer.

Report monotonous errors in The method selected in item setting shall be “Logarithmic
testing T3 and T4 items. regression”.
In plate arrangement, the standard articles shall be
arranged in the same line and from left to right in
Gradient disposition.

Report “no Too long distance between the Hall module at the bottom
predetermined-position attainment right corner of the front-end board and the magnetic ball of
signal for the enzyme label plate” the plate courier. Adjust the position of the Hall module.
when start the machine. The photoelectric coupler. Replace the photoelectric
coupler.
The front-end board is broken. Replace the front-end
board.

Report “no reset signal for the The position of the driving motor is deviated. Readjust it
enzyme label plate” when start the and fix it.
machine. The optical grating bar fell off. Glue the optical grating bar.
The drive board is broken. Replace the drive board.

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Data can not be transferred to the The serial port cord is broken. Replace the serial port cord
computer. (the normal serial port twisted-wire shall be 2-3, 3-2 and
5-5).
Data transference shall be conducted in the main menu of
the software.
External software error. Reinstall the software.

Logarithmic regression testing The setting of 0 density standard in logarithmic regression

curve is in confusion. is unnecessary. Remove the 0 density standard from item
setting.
In plate arrangement, the standard articles shall be
arranged in the same line and from left to right in
gradient disposition.
software upgrading error. Fail to Re-upgrade the software
start the machine.
When start the machine, the
The reverse-converter on the transfer board is damaged.
mouse appears, but soon after, it
Replace the reverse-converter.
flashes and then disappears, and
the screen becomes a blue
screen.
The testing results are on the high Recommend the use of double-wavelength method of
side. testing. Select 630 as the secondary wavelength.

Report “FLASH self-check error” The Flash ROM on the mainboard is broken. Replace it.
when start the machine.
No display when printing the
System software error. Update the system software.
synthetic report for the testing
item.
Report “the front-end command is The power supply board’s 5V voltage is on the low side.
overtime” in the self-check when Replace the V18 on the power supply board.
start the machine.
Fail to start the machine. The Irregular power supply. Replace the power supply unit
lamp is flashing.
Report “please check the
light-chopper’s motor” in the The circuit between the 1st pin of U8 on the front-end
self-check when start the board and the drive board is broken. Reconnect it.
machine.

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Appendix I Data transfer protocol of RT-2100C

Plate transfer data format:

str.Format(L"B,%s,%s,%d,%s,%s,%d,%d,%s,%s,%s,%s,%s,%s,%d,%.3f,%.3f,%.3f,%.3f,
%.3f,%.3f,%.3f,%d,%d,%.4x,%s"
B(Plate Sign)plate number, cuvette, plate item number, item name, full name, １
wavelength,２wavelength, sample number, absorbency, quantitative, qualitative, calculate
mode, reagent name, control 2 represent double controls 3 represent three controls,
factor, negative control, positive control, maximum normal value, minimum normal value,
negative suspicious range, positive suspicious range, positive 0 negative 1 no 2,
shake time, checkout code, TRANSFER FINISH

Quality control data transfer

str. Format(L"Q,%s,%s,%d,%s,%.4x,%s"
//Q（quality control sign）,assay name, method, month, data (include 31 days),checkout
code，TRANSFER FINISH

Sample data transfer

R, %s,%s,%d,%d,%s,%03d,%s,%s,%f,%s,%s,%f,%-7.3f,%7.3f,%.4x
R, patient name，section office name，gender of patient, age，time for test，sample
number，assay name，full name，unit，qualitative，quantitative，normal range (low)，
normal range (high)，checkout code，TRANSFER FINISH

TRANSFER FINISH is displayed when all the data is done.

port：
baud rate：19200
verify：Odd
data bit：8
stop bit：1

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