Exercise 1
Exercise 1
Exercise 1
EXERCISE NO. 1
INTRODUCTION
OBJECTIVES
MATERIALS
PROCEDURE
1. Using your collected materials, design a model each for prokaryotic and eukaryotic
replication.
2. Include all elements that converge at the replication fork during DNA replication for
both prokaryotes and eukaryotes.
3. Group the plastic chips according to color. One color represents one specific
replisome component.
4. Design your replication fork models and place the colored chips in their
corresponding places.
5. Make a legend of your colored chips for reference purposes.
6. Take pictures of your models and include these in your final reports.
1. Using the same set of materials, construct models for prokaryotic and eukaryotic
initiation of transcription/translation and post-transcriptional processing (in
eukaryotes).
2. Do the same for this exercise.
2
SKETCHES
LEGEND:
Violet = Replicated strand
White = Topoisomerase
Blue = Clamp
Green = Polymerase
Red = Helicase
Pink = Primer
Yellow = Parent Strand
Black = SSBP
LEGEND:
Violet = Replicated strand
White = Topoisomerase
Blue = Clamp
Green = Polymerase
Red = Helicase
Pink = Ligase
Yellow = Parent Strand
Orange = Primer
Black = SSBP
QUESTIONS
3. What are spliceosomes? What vital functions do they perform? And what are the
consequences if, in the performance of their functions, they go awry?