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Bbt312 Report 2

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72 views3 pages

Bbt312 Report 2

Uploaded by

entrepreneur881
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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based on the isolation, deteminaton of of DNA extraction The experiment is quantification, and purity bacteria) DNA. The purpose 18 40 obtain DNA by disrupting atl the cell ghuchyres , for example, cell wall, Cell membrans and the qucicar membrane. the. alisruption Of the pucleor membrane releases the highly Hon al h DNA Inte +he Intae removal of comaminating biomole euch as proteins, polysaccharides lipids, phenol and other secondary metabolits [4] @ Date given — * Arca ~ 28 (acepHd of “pure DNA D ~ 9.0-+(accepied as "pure" RNA) + Kaco/aeo, © £1. (protein contamination) = 34.8 (RNA contamination) Discuss! on. ng t 4he data given, the comparison | cined and thereto concluded hot the DNA rene| OF. other sink corarsi namon: The accord peturen THe rewhs obt calibre, it car OE somple rad pro absorbance ratio Bo the sormple 1s 2.01 which ts VE Jhon the ‘Pe! DNA rato which 16 1:2 at aco and 2800m less approximately. DNA extroction has great mportance 10 mony fields: For example, forensics 19 order to Javestigat. criminals as o suspect Roternity gests heleftt for qletermining th paternity © a child ,ancestry tracking smedical 4ests 5 genetic engineering and Vaccines L381 =e reasons behind contamination Beer cniepiecting: oS, samples because sample has higher cAnace. of getting contaminated fF not propetly handled skipped or chemicats oaded more HOO onceL3}.in meen ane he kacterial colony wos : aa into the pes buffer, the loop Should be properly sterilized 4o avoid any per possible errors include contaminaton. ¢ the pellet nat fully dissolving ushile vortex wos done; the Sample cidn'+ urderge complete Weis and DNA deqradehom can also occur “et at room dem perature if ONA san This “16 berau' fernperature has o significant effect on the Amount of BNA — lower demperature gives Qreater amount of ONA.

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