Embryo Quality Assessment: Which One To Choose???
Embryo Quality Assessment: Which One To Choose???
Embryo Quality Assessment: Which One To Choose???
DAY 0 1 2 3 4 5
pn
NPB’s
SCOTT et. al., 2003 Reprod Biomed Online
26 ± 1 h post-ICSI
Early cleavage check 2-cell stage
28 ± 1 h post-IVF
Symmetric Asymmetric
cleavage Multinucleation vacuolization
cleavage
ISTANBUL CONSENSUS,
2011. Human Reprod.
BLASTOCYST GRADING DAY-5
•An optimal embryo at this stage of development, will be a fully expanded with an
ICM (easily discernible and consisting of many cells) with trophectoderm comprises
Of many cells forming a cohesive epithelium.
•In literature it is agreed that ICM and TE has high prognostic value for implantation
and fetal development.
• TLM for embryo selection is one such technique that allows selection & de-
selection of embryos based on various morphokinetic parameters.
Time-lapse microscopy:
automated image acquisition every 5–20 min
• Excellent quality control and research tool & also allows off-site
data analysis
Time Lapse: Potential Concerns
• Safety regarding use in routine laboratory workflow need is concern,
Cost Effectiveness
• Correlations between gross morphology and implantation potential can be weak
and inaccurate