DNA microarray

From Wikipedia, the free encyclopedia

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.
Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of
a genome. Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). These
can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti­sense RNA) sample
(called target) under high­stringency conditions. Probe­target hybridization is usually detected and quantified by detection of fluorophore­,
silver­, or chemiluminescence­labeled targets to determine relative abundance of nucleic acid sequences in the target.

Contents
1 Principle
2 Uses and types
2.1 Fabrication
2.2 Spotted vs. in situ synthesised arrays
2.3 Two­channel vs. one­channel detection
2.4 A typical protocol
3 Microarrays and bioinformatics
3.1 Experimental design
3.2 Standardization
3.3 Data analysis
3.4 Annotation
3.5 Data warehousing
4 Alternative technologies
5 Multi­stranded DNA Microarray
6 Glossary
7 See also
8 References
9 External links

Principle
The core principle behind microarrays is hybridization between two DNA
strands, the property of complementary nucleic acid sequences to
specifically pair with each other by forming hydrogen bonds between
complementary nucleotide base pairs. A high number of complementary
base pairs in a nucleotide sequence means tighter non­covalent bonding
between the two strands. After washing off non­specific bonding
sequences, only strongly paired strands will remain hybridized.
Fluorescently labeled target sequences that bind to a probe sequence
generate a signal that depends on the hybridization conditions (such as
temperature), and washing after hybridization. Total strength of the signal,
from a spot (feature), depends upon the amount of target sample binding
to the probes present on that spot. Microarrays use relative quantitation in
which the intensity of a feature is compared to the intensity of the same
feature under a different condition, and the identity of the feature is Hybridization of the target to the probe.
known by its position.

The steps required in a microarray experiment.

Uses and types

Many types of arrays exist and the broadest distinction is whether they are spatially arranged on a surface or on coded beads:
 The traditional solid­phase array is a collection of orderly microscopic "spots", called features, each with
thousands of identical and specific probes attached to a solid surface, such as glass, plastic or silicon
biochip (commonly known as a genome chip, DNA chip or gene array). Thousands of these features can be
placed in known locations on a single DNA microarray.
The alternative bead array is a collection of microscopic polystyrene beads, each with a specific probe and
a ratio of two or more dyes, which do not interfere with the fluorescent dyes used on the target sequence.

DNA microarrays can be used to detect DNA (as in comparative genomic hybridization), or detect RNA (most
commonly as cDNA after reverse transcription) that may or may not be translated into proteins. The process of
Two Affymetrix chips. A
measuring gene expression via cDNA is called expression analysis or expression profiling.
match is shown at bottom
Applications include: left for size comparison.

Application or
Synopsis
technology
In an mRNA or gene expression profiling experiment the expression levels of thousands of genes are simultaneously
monitored to study the effects of certain treatments, diseases, and developmental stages on gene expression. For
Gene expression
example, microarray­based gene expression profiling can be used to identify genes whose expression is changed in
profiling response to pathogens or other organisms by comparing gene expression in infected to that in uninfected cells or
tissues.[1]
Comparative
genomic Assessing genome content in different cells or closely related organisms.[2][3]
hybridization
Small microarrays to check IDs of organisms in food and feed (like GMO [1] (http://bgmo.jrc.ec.europa.eu/home/doc
GeneID s.htm)), mycoplasms in cell culture, or pathogens for disease detection, mostly combining PCR and microarray
technology.
DNA sequences bound to a particular protein can be isolated by immunoprecipitating that protein (ChIP), these
Chromatin fragments can be then hybridized to a microarray (such as a tiling array) allowing the determination of protein binding
immunoprecipitation site occupancy throughout the genome. Example protein to immunoprecipitate are histone modifications (H3K27me3,
on Chip H3K4me2, H3K9me3, etc.), Polycomb­group protein (PRC2:Suz12, PRC1:YY1) and trithorax­group protein (Ash1)
to study the epigenetic landscape or RNA Polymerase II to study the transcription landscape.
Analogously to ChIP, genomic regions bound by a protein of interest can be isolated and used to probe a microarray
to determine binding site occupancy. Unlike ChIP, DamID does not require antibodies but makes use of adenine
DamID
methylation near the protein's binding sites to selectively amplify those regions, introduced by expressing minute
amounts of protein of interest fused to bacterial DNA adenine methyltransferase.
Identifying single nucleotide polymorphism among alleles within or between populations.[4] Several applications of
SNP detection microarrays make use of SNP detection, including genotyping, forensic analysis, measuring predisposition to disease,
identifying drug­candidates, evaluating germline mutations in individuals or somatic mutations in cancers, assessing
loss of heterozygosity, or genetic linkage analysis.
An exon junction array design uses probes specific to the expected or potential splice sites of predicted exons for a
gene. It is of intermediate density, or coverage, to a typical gene expression array (with 1­3 probes per gene) and a
Alternative splicing
genomic tiling array (with hundreds or thousands of probes per gene). It is used to assay the expression of alternative
detection
splice forms of a gene. Exon arrays have a different design, employing probes designed to detect each individual exon
for known or predicted genes, and can be used for detecting different splicing isoforms.
A Fusion gene microarray can detect fusion transcripts, e.g. from cancer specimens. The principle behind this is
Fusion genes
building on the alternative splicing microarrays. The oligo design strategy enables combined measurements of
microarray
chimeric transcript junctions with exon­wise measurements of individual fusion partners.
Genome tiling arrays consist of overlapping probes designed to densely represent a genomic region of interest,
Tiling array sometimes as large as an entire human chromosome. The purpose is to empirically detect expression of transcripts or
alternatively spliced forms which may not have been previously known or predicted.
Right­handed double­stranded B­DNA microarrays can be used to characterize novel drugs and biologicals that can be
Double­stranded B­ employed to bind specific regions of immobilized, intact, double­stranded DNA. This approach can be used to inhibit
DNA microarrays gene expression.[5][6][7] They also allow for characterization of their structure under different environmental
conditions.
Left­handed double­stranded Z­DNA microarrays can be used to identify short sequences of the alternative Z­DNA
Double­stranded Z­ structure located within longer stretches of right­handed B­DNA genes (e.g., transcriptional enhancement,
DNA microarrays recombination, RNA editing).[5][6][7][8] The microarrays also allow for characterization of their structure under
different environmental conditions.
Multi­stranded DNA Multi­stranded DNA and RNA microarrays can be used to identify novel drugs that bind to these multi­stranded
microarrays (triplex­ nucleic acid sequences. This approach can be used to discover new drugs and biologicals that have the ability to
DNA microarrays
and quadruplex­ inhibit gene expression.[5][6][7][9][10] These microarrays also allow for characterization of their structure under different
DNA microarrays) environmental conditions.

Fabrication
Microarrays can be manufactured in different ways, depending on the number of probes under examination, costs, customization
requirements, and the type of scientific question being asked. Arrays may have as few as 10 probes or up to 2.1 million micrometre­scale
probes from commercial vendors.

Spotted vs. in situ synthesised arrays

Microarrays can be fabricated using a variety of technologies, including printing with

fine­pointed pins onto glass slides, photolithography using pre­made masks,
photolithography using dynamic micromirror devices, ink­jet printing,[11][12] or
electrochemistry on microelectrode arrays.

In spotted microarrays, the probes are oligonucleotides, cDNA or small fragments of

PCR products that correspond to mRNAs. The probes are synthesized prior to deposition
on the array surface and are then "spotted" onto glass. A common approach utilizes an
array of fine pins or needles controlled by a robotic arm that is dipped into wells
containing DNA probes and then depositing each probe at designated locations on the
array surface. The resulting "grid" of probes represents the nucleic acid profiles of the
prepared probes and is ready to receive complementary cDNA or cRNA "targets" derived
from experimental or clinical samples. This technique is used by research scientists
A DNA microarray being printed by a robot at the
around the world to produce "in­house" printed microarrays from their own labs. These
University of Delaware
arrays may be easily customized for each experiment, because researchers can choose the
probes and printing locations on the arrays, synthesize the probes in their own lab (or
collaborating facility), and spot the arrays. They can then generate their own labeled samples for hybridization, hybridize the samples to the
array, and finally scan the arrays with their own equipment. This provides a relatively low­cost microarray that may be customized for each
study, and avoids the costs of purchasing often more expensive commercial arrays that may represent vast numbers of genes that are not of
interest to the investigator. Publications exist which indicate in­house spotted microarrays may not provide the same level of sensitivity
compared to commercial oligonucleotide arrays,[13] possibly owing to the small batch sizes and reduced printing efficiencies when compared
to industrial manufactures of oligo arrays.

In oligonucleotide microarrays, the probes are short sequences designed to match parts of the sequence of known or predicted open reading
frames. Although oligonucleotide probes are often used in "spotted" microarrays, the term "oligonucleotide array" most often refers to a
specific technique of manufacturing. Oligonucleotide arrays are produced by printing short oligonucleotide sequences designed to represent a
single gene or family of gene splice­variants by synthesizing this sequence directly onto the array surface instead of depositing intact
sequences. Sequences may be longer (60­mer probes such as the Agilent design) or shorter (25­mer probes produced by Affymetrix)
depending on the desired purpose; longer probes are more specific to individual target genes, shorter probes may be spotted in higher density
across the array and are cheaper to manufacture. One technique used to produce oligonucleotide arrays include photolithographic synthesis
(Affymetrix) on a silica substrate where light and light­sensitive masking agents are used to "build" a sequence one nucleotide at a time
across the entire array.[14] Each applicable probe is selectively "unmasked" prior to bathing the array in a solution of a single nucleotide, then
a masking reaction takes place and the next set of probes are unmasked in preparation for a different nucleotide exposure. After many
repetitions, the sequences of every probe become fully constructed. More recently, Maskless Array Synthesis from NimbleGen Systems has
combined flexibility with large numbers of probes.[15]

Two­channel vs. one­channel detection

Two­color microarrays or two­channel microarrays are typically hybridized with cDNA prepared
from two samples to be compared (e.g. diseased tissue versus healthy tissue) and that are labeled with
two different fluorophores.[16] Fluorescent dyes commonly used for cDNA labeling include Cy3,
which has a fluorescence emission wavelength of 570 nm (corresponding to the orange part of the
light spectrum), and Cy5 with a fluorescence emission wavelength of 670 nm (corresponding to the
red part of the light spectrum). The two Cy­labeled cDNA samples are mixed and hybridized to a
single microarray that is then scanned in a microarray scanner to visualize fluorescence of the two
fluorophores after excitation with a laser beam of a defined wavelength. Relative intensities of each
fluorophore may then be used in ratio­based analysis to identify up­regulated and down­regulated
genes.[17]

Oligonucleotide microarrays often carry control probes designed to hybridize with RNA spike­ins.
The degree of hybridization between the spike­ins and the control probes is used to normalize the
hybridization measurements for the target probes. Although absolute levels of gene expression may be
determined in the two­color array in rare instances, the relative differences in expression among
different spots within a sample and between samples is the preferred method of data analysis for the
two­color system. Examples of providers for such microarrays includes Agilent with their Dual­Mode
platform, Eppendorf with their DualChip platform for colorimetric Silverquant labeling, and
Diagram of typical dual­colour
TeleChem International with Arrayit.
microarray experiment.
In single­channel microarrays or one­color microarrays, the arrays provide intensity data for each
probe or probe set indicating a relative level of hybridization with the labeled target. However, they do not truly indicate abundance levels of
a gene but rather relative abundance when compared to other samples or conditions when processed in the same experiment. Each RNA
molecule encounters protocol and batch­specific bias during amplification, labeling, and hybridization phases of the experiment making
comparisons between genes for the same microarray uninformative. The comparison of two conditions for the same gene requires two
separate single­dye hybridizations. Several popular single­channel systems are the Affymetrix "Gene Chip", Illumina "Bead Chip", Agilent
single­channel arrays, the Applied Microarrays "CodeLink" arrays, and the Eppendorf "DualChip & Silverquant". One strength of the single­
dye system lies in the fact that an aberrant sample cannot affect the raw data derived from other samples, because each array chip is exposed
to only one sample (as opposed to a two­color system in which a single low­quality sample may drastically impinge on overall data precision
even if the other sample was of high quality). Another benefit is that data are more easily compared to arrays from different experiments as
long as batch effects have been accounted for.

One channel microarray may be the only choice in some situations. Suppose samples need to be compared: then the number of experiments
required using the two channel arrays quickly becomes unfeasible, unless a sample is used as a reference.

number of samples one­channel microarray two channel microarray two channel microarray (with reference)

1 1 1 1
2 2 1 1
3 3 3 2
4 4 6 3

A typical protocol

This is an example of a DNA microarray experiment, detailing a particular case to better explain DNA microarray experiments, while
enumerating possible alternatives.

1. The two samples to be compared (pairwise comparison) are grown/acquired. In this example treated sample (case) and untreated
sample (control).
2. The nucleic acid of interest is purified: this can be all RNA for expression profiling, DNA for comparative hybridization, or DNA/RNA
bound to a particular protein which is immunoprecipitated (ChIP­on­chip) for epigenetic or regulation studies. In this example total
RNA is isolated (total as it is nuclear and cytoplasmic) by Guanidinium thiocyanate­phenol­chloroform extraction (e.g. Trizol) which
isolates most RNA (whereas column methods have a cut off of 200 nucleotides) and if done correctly has a better purity.
3. The purified RNA is analysed for quality (by capillary electrophoresis) and quantity (for example, by using a NanoDrop or
NanoPhotometer spectrometer). If the material is of acceptable quality and sufficient quantity is present (e.g., >1μg, although the
required amount varies by microarray platform), the experiment can proceed.
4. The labelled product is generated via reverse transcription and sometimes with an optional PCR amplification. The RNA is reverse
transcribed with either polyT primers (which amplify only mRNA) or random primers (which amplify all RNA, most of which is
rRNA); miRNA microarrays ligate an oligonucleotide to the purified small RNA (isolated with a fractionator), which is then reverse
transcribed and amplified. The label is added either during the reverse transcription step, or following amplification if it is performed.
The sense labelling is dependent on the microarray; e.g. if the label is added with the RT mix, the cDNA is antisense and the
microarray probe is sense, except in the case of negative controls. The label is typically fluorescent; only one machine uses radiolabels.
The labelling can be direct (not used) or indirect (requires a coupling stage). For two­channel arrays, the coupling stage occurs before
hybridization, using aminoallyl uridine triphosphate (aminoallyl­UTP, or aaUTP) and NHS amino­reactive dyes (such as cyanine dyes);
for single­channel arrays, the coupling stage occurs after hybridization, using biotin and labelled streptavin. The modified nucleotides
(usually in a ratio of 1 aaUTP: 4 TTP (thymidine triphosphate)) are added enzymatically in a low ratio to normal nucleotides, typically
resulting in 1 every 60 bases. The aaDNA is then purified with a column (using a phosphate buffer solution, as Tris contains amine
groups). The aminoallyl group is an amine group on a long linker attached to the nucleobase, which reacts with a reactive dye. A form
of replicate known as a dye flipcan be performed to remove any dye effects in two­channel experiments; for a dye flip, a second slide is
used, with the labels swapped (the sample that was labeled with Cy3 in the first slide is labeled with Cy5, and vice versa). In this
example, aminoallyl­UTP is present in the reverse­transcribed mixture.
5. The labeled samples are then mixed with a propriety hybridization solution which can consist of SDS, SSC, dextran sulfate, a blocking
agent (such as COT1 DNA, salmon sperm DNA, calf thymus DNA, PolyA or PolyT), Denhardt's solution, or formamine.
6. The mixture is denatured and added to the pinholes of the microarray. The holes are sealed and the microarray hybridized, either in a
hyb oven, where the microarray is mixed by rotation, or in a mixer, where the microarray is mixed by alternating pressure at the
pinholes.
7. After an overnight hybridization, all nonspecific binding is washed off (SDS and SSC).
8. The microarray is dried and scanned by a machine that uses a laser to excite the dye and measures the emission levels with a detector.
9. The image is gridded with a template and the intensities of each feature (composed of several pixels) is quantified.
10. The raw data is normalized; the simplest normalization method is to subtract background intensity and scale so that the total intensities
of the features of the two channels are equal, or to use the intensity of a reference gene to calculate the t­value for all of the intensities.
More sophisticated methods include z­ratio, loess and lowess regression and RMA (robust multichip analysis) for Affymetrix chips
(single­channel, silicon chip, in situ synthesised short oligonucleotides).

Microarrays and bioinformatics

The advent of inexpensive microarray experiments created several specific bioinformatics challenges:

the multiple levels of replication in experimental design (Experimental design)

the number of platforms and independent groups and data format (Standardization)
the statistical treatment of the data (Statistical analysis)
mapping each probe to the mRNA transcript that it measures (Annotation)
 the sheer volume of data and the ability to share it (Data warehousing)

Experimental design

Due to the biological complexity of gene expression, the considerations of experimental design that are
discussed in the expression profiling article are of critical importance if statistically and biologically valid
conclusions are to be drawn from the data.

There are three main elements to consider when designing a microarray experiment. First, replication of the
biological samples is essential for drawing conclusions from the experiment. Second, technical replicates (two Gene expression values
RNA samples obtained from each experimental unit) help to ensure precision and allow for testing differences from microarray
within treatment groups. The biological replicates include independent RNA extractions and technical experiments can be
replicates may be two aliquots of the same extraction. Third, spots of each cDNA clone or oligonucleotide are represented as heat maps to
present as replicates (at least duplicates) on the microarray slide, to provide a measure of technical precision in visualize the result of data
each hybridization. It is critical that information about the sample preparation and handling is discussed, in analysis.
order to help identify the independent units in the experiment and to avoid inflated estimates of statistical
significance.[18]

Standardization

Microarray data is difficult to exchange due to the lack of standardization in platform fabrication, assay protocols, and analysis methods. This
presents an interoperability problem in bioinformatics. Various grass­roots open­source projects are trying to ease the exchange and analysis
of data produced with non­proprietary chips:

For example, the "Minimum Information About a Microarray Experiment" (MIAME) checklist helps define the level of detail that
should exist and is being adopted by many journals as a requirement for the submission of papers incorporating microarray results. But
MIAME does not describe the format for the information, so while many formats can support the MIAME requirements, as of 2007 no
format permits verification of complete semantic compliance.
The "MicroArray Quality Control (MAQC) Project" is being conducted by the US Food and Drug Administration (FDA) to develop
standards and quality control metrics which will eventually allow the use of MicroArray data in drug discovery, clinical practice and
regulatory decision­making.[19]
The MGED Society has developed standards for the representation of gene expression experiment results and relevant annotations.

Data analysis

Microarray data sets are commonly very large, and analytical precision is influenced by a number of variables. Statistical challenges include
taking into account effects of background noise and appropriate normalization of the data. Normalization methods may be suited to specific
platforms and, in the case of commercial platforms, the analysis may be proprietary. Algorithms that affect statistical analysis include:

Image analysis: gridding, spot recognition of the scanned image (segmentation algorithm), removal or marking of poor­quality and
low­intensity features (called flagging).
Data processing: background subtraction (based on global or local background), determination of spot intensities and intensity ratios,
visualisation of data (e.g. see MA plot), and log­transformation of ratios, global or local normalization of intensity ratios, and
segmentation into different copy number regions using step detection algorithms.[20]
Class discovery analysis: This analytic approach, sometimes called unsupervised classification or knowledge discovery, tries to identify
whether microarrays (objects, patients, mice, etc.) or genes cluster together in groups. Identifying naturally existing groups of objects
(microarrays or genes) which cluster together can enable the discovery of new groups that otherwise were not previously known to
exist. During knowledge discovery analysis, various unsupervised classification techniques can be employed with DNA microarray
data to identify novel clusters (classes) of arrays.[21] This type of approach is not hypothesis­driven, but rather is based on iterative
pattern recognition or statistical learning methods to find an "optimal" number of clusters in the data. Examples of unsupervised
analyses methods include self­organizing maps, neural gas, k­means cluster analyses,[22] hierarchical cluster analysis, Genomic Signal
Processing based clustering[23] and model­based cluster analysis. For some of these methods the user also has to define a distance
measure between pairs of objects. Although the Pearson correlation coefficient is usually employed, several other measures have been
proposed and evaluated in the literature.[24] The input data used in class discovery analyses are commonly based on lists of genes
having high informativeness (low noise) based on low values of the coefficient of variation or high values of Shannon entropy, etc. The
determination of the most likely or optimal number of clusters obtained from an unsupervised analysis is called cluster validity. Some
commonly used metrics for cluster validity are the silhouette index, Davies­Bouldin index,[25] Dunn's index, or Hubert's statistic.
Class prediction analysis: This approach, called supervised classification, establishes the basis for developing a predictive model into
which future unknown test objects can be input in order to predict the most likely class membership of the test objects. Supervised
analysis[21] for class prediction involves use of techniques such as linear regression, k­nearest neighbor, learning vector quantization,
decision tree analysis, random forests, naive Bayes, logistic regression, kernel regression, artificial neural networks, support vector
machines, mixture of experts, and supervised neural gas. In addition, various metaheuristic methods are employed, such as genetic
algorithms, covariance matrix self­adaptation, particle swarm optimization, and ant colony optimization. Input data for class prediction
are usually based on filtered lists of genes which are predictive of class, determined using classical hypothesis tests (next section), Gini
diversity index, or information gain (entropy).
Hypothesis­driven statistical analysis: Identification of statistically significant changes in gene expression are commonly identified
using the t­test, ANOVA, Bayesian method[26] Mann–Whitney test methods tailored to microarray data sets, which take into account
multiple comparisons[27] or cluster analysis.[28] These methods assess statistical power based on the variation present in the data and the
number of experimental replicates, and can help minimize Type I and type II errors in the analyses.[29]
 Dimensional reduction: Analysts often reduce the number of dimensions (genes) prior to data analysis.[21] This may involve linear
approaches such as principal components analysis (PCA), or non­linear manifold learning (distance metric learning) using kernel PCA,
diffusion maps, Laplacian eigenmaps, local linear embedding, locally preserving projections, and Sammon's mapping.
Network­based methods: Statistical methods that take the underlying structure of gene networks into account, representing either
associative or causative interactions or dependencies among gene products.[30] Weighted gene co­expression network analysis is widely
used for identifying co­expression modules and intramodular hub genes. Modules may corresponds to cell types or pathways. Highly
connected intramodular hubs best represent their respective modules.

Microarray data may require further processing aimed at reducing the dimensionality of the data to aid comprehension and more focused
analysis.[31] Other methods permit analysis of data consisting of a low number of biological or technical replicates; for example, the Local
Pooled Error (LPE) test pools standard deviations of genes with similar expression levels in an effort to compensate for insufficient
replication.[32]

Annotation

The relation between a probe and the mRNA that it is expected to detect is not trivial. [33] Some mRNAs may cross­hybridize probes in the
array that are supposed to detect another mRNA. In addition, mRNAs may experience amplification bias that is sequence or molecule­
specific. Thirdly, probes that are designed to detect the mRNA of a particular gene may be relying on genomic EST information that is
incorrectly associated with that gene.

Data warehousing

Microarray data was found to be more useful when compared to other similar datasets. The sheer volume of data, specialized formats (such as
MIAME), and curation efforts associated with the datasets require specialized databases to store the data. A number of open­source data
warehousing solutions, such as InterMine and BioMart (http://www.biomart.org/), have been created for the specific purpose of integrating
diverse biological datasets, and also support analysis.

Alternative technologies
Advances in massively parallel sequencing has led to the development of RNA­Seq technology, that enables a whole transcriptome shotgun
approach to characterize and quantify gene expression.[34][35] Unlike microarrays, which need a reference genome and transcriptome to be
available before the microarray itself can be designed, RNA­Seq can also be used for new model organisms whose genome has not been
sequenced yet.[35]

Multi­stranded DNA Microarray

[36]This figure shows the anchoring of eight different

DNA and RNA molecules onto a DNA microarray
surface.

Glossary
An Array or slide is a collection of features spatially arranged in a two dimensional grid, arranged in columns and rows.
Block or subarray: a group of spots, typically made in one print round; several subarrays/blocks form an array.
Case/control: an experimental design paradigm especially suited to the two­colour array system, in which a condition chosen as
control (such as healthy tissue or state) is compared to an altered condition (such as a diseased tissue or state).
Channel: the fluorescence output recorded in the scanner for an individual fluorophore and can even be ultraviolet.
Dye flip or Dye swap or Fluor reversal: reciprocal labelling of DNA targets with the two dyes to account for dye bias in experiments.
Scanner: an instrument used to detect and quantify the intensity of fluorescence of spots on a microarray slide, by selectively exciting
fluorophores with a laser and measuring the fluorescence with a filter (optics) photomultiplier system.
Spot or feature: a small area on an array slide that contains picomoles of specific DNA samples.
For other relevant terms see:

Glossary of gene expression terms

Protocol (natural sciences)
See also
Cyanine dyes, such as Cy3 and Cy5, are commonly used Phenotype microarray
fluorophores with microarrays Systems biology
Gene chip analysis Serial analysis of gene expression
Significance analysis of microarrays RNA­Seq
Methylation specific oligonucleotide microarray Whole genome sequencing
Microfluidics or lab­on­chip
Pathogenomics
Phenotype microarray
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