LOCUS AJ002680 3941 bp DNA linear SYN 01-SEP-2004

DEFINITION Synthetic DNA containing Escherichia coli plasmid pFA6a-kanMX4,

kanamycin and geneticin (G418) resistance gene, bla gene, multiple
cloning site, pSP72 derivative.
ACCESSION AJ002680
VERSION AJ002680.1 GI:2623975
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1
AUTHORS Oka,A., Sugisaki,H. and Takanami,M.
TITLE Nucleotide sequence of the kanamycin resistance transposon Tn903
JOURNAL J. Mol. Biol. 147 (2), 217-226 (1981)
PUBMED 6270337
REFERENCE 2
AUTHORS Steiner,S. and Philippsen,P.
TITLE Sequence and promoter analysis of the highly expressed TEF gene of
the filamentous fungus Ashbya gossypii
JOURNAL Mol. Gen. Genet. 242 (3), 263-271 (1994)
PUBMED 8107673
REFERENCE 3
AUTHORS Wach,A., Brachat,A., Pohlmann,R. and Philippsen,P.
TITLE New heterologous modules for classical or PCR-based gene
disruptions in Saccharomyces cerevisiae
JOURNAL Yeast 10 (13), 1793-1808 (1994)
PUBMED 7747518
REFERENCE 4 (bases 1 to 3941)
AUTHORS Philippsen,P.
TITLE Direct Submission
JOURNAL Submitted (07-NOV-1997) Philippsen P., Applied Microbiology,
University of Basel, Biozentrum, Klingelbergstr. 70, Basel,
CH-4056, SWITZERLAND
FEATURES Location/Qualifiers
source 1..3941
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
/focus
source 80..482
/organism="Eremothecium gossypii"
/mol_type="other DNA"
/db_xref="taxon:33169"
source 483..1271
/organism="Escherichia coli"
/mol_type="other DNA"
/db_xref="taxon:562"
source 1277..1501
/organism="Eremothecium gossypii"
/mol_type="other DNA"
/db_xref="taxon:33169"
misc_feature 1..79
/note="artificial sequence"
misc_feature 5..75
/note="multiple cloning site"
/citation=[3]
gene 115..458
/gene="TEF"
promoter 115..458
 /gene="TEF"
/note="Translation elongation factor 1 alpha"
/citation=[2]
CDS 459..1268
/note="unnamed protein product; Kanamycin resistance gene"
/citation=[1]
/codon_start=1
/transl_table=11
/protein_id="CAA05683.1"
/db_xref="GI:2623976"
/db_xref="InterPro:IPR002575"
/db_xref="InterPro:IPR008266"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR012149"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
repeat_region 483..1271
/mobile_element="transposon:Tn903"
misc_feature 1272..1276
/note="artificial sequence"
gene 1277..1471
/gene="TEF"
terminator 1277..1471
/gene="TEF"
/note="translation elongation factor 1 alpha"
/citation=[2]
misc_feature 1502..3941
/note="artificial sequence"
ORIGIN
1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
61 aggcgcgcca gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
121 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
181 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
241 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
301 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
361 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
421 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg
481 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
541 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
601 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
661 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
721 ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat
781 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
841 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
901 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
961 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
1021 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
1081 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
1141 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
1201 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
1261 ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
1321 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
1381 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
1441 caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc
1501 agtgtcgaaa acgagctcga attcatcgat gatatcagat ccactagtgg cctatgcggc
1561 cgcggatctg ccggtctccc tatagtgagt cgtattaatt tcgataagcc aggttaacct
 1621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
1681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
1741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
2041 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
2101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
2221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
2281 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
2341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
2401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
2461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
2581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
2641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
2701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
2761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
2821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
2941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
3001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
3061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
3121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
3181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
3241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
3301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
3361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
3421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
3481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3601 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
3661 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
3721 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
3781 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
3841 tgtactgaga gtgcaccata tggacatatt gtcgttagaa cgcggctaca attaatacat
3901 aaccttatgt atcatacaca tacgatttag gtgacactat a
//