Thesis For Research Proposal-Msa
Thesis For Research Proposal-Msa
) TO VARIOUS
BIONUTRIENT APPLICATION
__________________________________________________
A Research Proposal
Presented to
ARLENE P. ABLOG, Ph.D.
Graduate School
Ilocos Sur Polytechnic State College, Sta. Maria Campus
______________________________________________
In Partial Fulfillment
of the Requirements for the Degree
MASTER OF SCIENCE IN AGRICULTURE
Major in Rural Development
1
CHAPTER 1
production in the world amounted to 105.372 million tons more than half of which were
terms of volume among other major vegetables grown. Watermelon is a native to Central
Africa but was grown by ancient Egyptians and is believed to have also been cultured in
Asia, Russia and the near Middle East, for a thousand of years now. Today, Southwest
The climatic conditions in the provinces of La Union, Ilocos Sur and Ilocos Norte
are favorable for watermelon production particularly during the onset of the dry season in
November (Sabado, 2005). The crop is usually planted after the first rice crop and
extends up to late January for late season crops. Watermelon has become a popular crop
among farmers because it demands a good market price and is a favorite dessert and
snack especially during the hot summer months of the year (Samonte, 2006). Farmers sell
their produce within the community and nearby provinces where watermelon is not
grown but supply is not sustained until the summer months where the demand could even
In 2015, a total of 2,396.7 metric tons was produced in Ilocos Sur from 128.86
Sto. Domingo and Sta. Lucia (OPAG, 2016). Production has declined in the past years
2
due to decreased yield. However, much of the decline has been due to reduced consumer
demand which has result of the sale of poor-quality watermelons, something that can be
obtained only with careful management, correct number of vines to be maintained and
Diana watermelon is a solo type F1 variety with oblong shape and unique, golden-
yellow skin. This is an excellent variety for high class markets. Its flesh is red with a
cottony, fluffy texture. Brix averages from 10% to 12% and is harvestable starting from
nowadays. It is important that watermelon yield in the province and in nearby producing
areas can be increased and the supply be maintained in order that the local markets can
meet the demand in terms of volume and fruit quality. Increased production and
improved quality would enable local producers to increase their income. The trend of
increasing demand in the local market is parallel with stable production system that
Most cucurbit crops like watermelon need a good production system. Two of the
most important aspects that would increase yield and improve quality include the right
have started to discover more native and organic plants in the surroundings to have the
3
Organic fertilizers are fertilizers that are mainly composed of organic or natural
fertilizers such as manures and potash. Organic fertilizers are composed of anything
biodegradable that is found in nature which releases nutrients as it decays (Diaz et al.,
2011).
It is the milieu that the researcher thinks of using organic foliar fertilizer such as
Fish Amino Acid (FAA), Fermented Plant Juice (FPJ) and Indigenous Micro Organism.
Fish Amino Acid (FAA) is organic fertilizer sprayed on leaves for its nitrogen and
trace minerals and used directly by plants. Also, the micro-nutrients in natural form are
immediately absorbed into leaf surface. Spraying on regular basis increases the health,
vigor and yield of plants due to easily absorbed additional nutrition (Indian Mart, 2012).
On the other hand, Fermented Plant Juice (FPJ) helps the plants because of its
aids in the assimilation of the plants. It enables better nutrient absorption hence, healthier
plant growth. IMO is said to be cheap because of the availability of the materials in
cultivating it. It can be collected in rice or in fermented vegetables like mustard. A large
diversity of IMO can be found in forests, bamboo grooves and areas with thick
the production of tomato have lessened the use of hazardous chemicals (Ferrer, 2016).
4
Various bio-nutrient application had also been studied in numerous crops and had
some positive response to yield and quality but not much as watermelon, hence, this is
proposal study.
sources (fish amino acids, fermented plant juice and indigenous microorganisms).
2. Determine the best bio-nutrient source that will give the best performance of the
3. Identify the appropriate bio-nutrient source that will give the higher fruit set and
yield.
them;
5
Watermelon growers/Farmers. This serves as additional knowledge to them.
They could apply this as their guide for growing watermelon and utilize these bio-
nutrients as their bridge to join hand in hand to increase their productivity and income.
Barang-ay Demofarm Project Staff on Plant Division. The results of the study
farmers.
6
DEFINITION OF TERMS
Abscission – the separation of a leaf, flower of fruit from a plant as a result of natural,
Amino Acid – a liquid made from fresh trash which contains abundant amounts of
Fermented Plant Juice – juice extracted from axillary buds and young fruits, fats
Indigenous Micro-Organism – are group of innate microbial consortium that inhabit the
soil and the surfaces of all living things inside and outside which have the potentiality in
Total Soluble Solid – the sugar content measures and includes the carbohydrates,
Watermelon – is a warm season annual that covers a large area with its
sprawling stem which bears oval or round fruits with a hard green or yellow skin and
sweet and juicy red, pink and yellow flesh usually with many black seeds.
7
REVIEW OF RELATED LITERATURE
This presents results of conducted studies and literature which serve as bases of
produce both male and female flowers and the size of the fruit varies from 2 to 14 kg,
depending on the variety. Watermelon is successfully grown under cold temperature. The
best average temperature for watermelon production is between 18ºC and 35ºC. Above
35ºC or below 16ºC temperature will slow the growth and affect the crops maturity.
Watermelon does well on sandy loam or silt loamy soils. Sandy soils have limited water-
holding capacities and must be carefully watered and fertilized to allow for high yield
potential. Soil pH which ranges from 5.8 to 6.6 is ideal for watermelon production.
Watermelon serves as food for human consumption. It is a fruit that provides large
amount of vitamins, minerals and antioxidants at low amount of calories. It contains fiber
which encourages healthy digestion. The consumption of watermelon decreases the risk
of obesity and overall mortality, diabetes and heart diseases. Vegetables constitute a
major source of nitrate, providing more than 85% of the average daily human dietary
Watermelons are warm season annuals and are less tolerant to cold weather than
other Cucurbits and Cantaloupe. They have long prostrate vines and thus, require a lot of
garden space for good yield (George, 2004). It requires well-drained soils that are rich in
8
organic matter with water retention capacity (Lawal, 2000). The seeds are rich in fat and
protein and are eaten as snacks and also added to other dishes or used as an oil seed.
NUTRIENTS QUANTITIES
Energy 30 kcal
also provides significant amount of vitamin B, as well as minerals such as potassium (K),
magnesium (Mg), iron (Fe), manganese (Mn), phosphorus (P), sodium (Na) and zinc (Zn)
(Wikipedia, 2013). The leaves and fruits are utilized as green vegetables (Wayne, 1990).
Watermelon is spaced widely in the farm, though bush varieties that require less spacing
tasty water and utilizes large amount of moisture to produce juicy flesh (George, 2004).
Some farmers who embark on large scale production of watermelon lack cultural
practices like pruning in its production. Efforts are required for appropriate cultural
9
Bio-Nutrient
Every plant like any organism needs certain components for growth over and
above the soil, sun, rain and air. The basic component of living cells is proteins, with
building block material, amino acids. Proteins are formed by sequence of Amino Acids
The application of amino acids for foliar use is based on its requirement by plants
in general and at critical stages of growth in particular. Plant absorbs amino acids through
Liquid) and foliar spray provide readymade building blocks for Protein synthesis.
Amino acids are nitrogen (N), part of the five macro-nutrient elements of
fertilizer. Fish amino acid is a liquid made from fish trash. It contains abundant amounts
of nutrients and various types of amino acids. It is absorbed directly by the crops and it
stimulates the activity of microorganism. If you add urea, you will have an even better
effect.
Herring was used in making Fish Amino Acid because it produces the largest amount of
fat which has a very strong smell that repels insects particularly Mythimna Separala
10
Walker. The fat from the fish was collected during the production process, mix with
Fish has been used as a natural source of plant nutrients for centuries. Fish
fertilizer provides a rich source of organic matter that breaks down and release nutrients
into the soil to enhance the strength and vigor of the plants. Additionally, naturally
occurring microbes thrive and work their best in soil rich in organic matter. Fish fertilizer
is ideal for use on all plants including vegetable garden, fruits, roses, shrubs, trees and
universities have concluded trials and it is concluded that when amino acid is sprayed in
cotton, chilies, groundnut, sunflower, tomato, potato, grapes, tea, coffee, paddy rice and
Ph 3.37
Nitrogen, N 0.58
Fish Fertilizers provide an excellent source of nutrition plants and the soil. When
fish fertilizers are used, the plants receive a controlled level of nitrogen, a vital element
necessary for the production of chlorophyll and maintaining the health of the plant. Too
11
much nitrogen, which can be a side effect of chemical fertilizers, can overwhelm the
plant and cause it to be more vulnerable to weather fluctuations, insects and diseases. It is
well known that synthetic nitrogen fertilizers ‘volatilize’ into the atmosphere, not only
being lost to plant availability, but contributing to greenhouse gases. Runoff of synthetic
fertilizers to the water table, aquifers, streams, rivers and our oceans are other negative
Another major benefit of using a fish fertilizer is improving a healthy food source
which stimulates microorganism that exist in the soil. Some forms of commonly found
soil microbes also synthesize nitrogen (e.g. nitrifying bacteria). When fed good food, they
increase in numbers and produce more available organic nitrogen for roots to uptake and
naturally feed the plant. An active soil ecosystem will improve the vitality of the crop and
increase production.
Based from the result of the study conducted by (Tomas, 2016) on the effect of
fish amino acid (FAA) on the yield performance of tomato revealed that plants sprayed
with fish amino acid produced significantly bigger polar (4.18 cm) and lateral (3.74 cm)
diameter of tomato fruits, produced more marketable fruits per plot (28.45) and non-
fertilized with fish amino acid produced more number of non-marketable fruits/plot
Fermented plant juice (FPJ) is derived from mixing the young shoots of kangkong
with molasses and/or crude sugar and fermented in one (1) week. FPJ is rich in micro and
12
macro-nutrients. The juice also contains microorganisms which gives strength to plants
and animals. The shoots of plants are difficult to dissolve in water or any kind of oil but it
can be done with a little amount of alcohol. The process of fermenting the soft parts of
the plants with molasses/crude sugar and with the presence of microorganisms will result
to a small per cent of alcohol which is responsible in extracting the juice from the young
Ph 3.37
Nitrogen, N 1.62
The part of the plants used for this fermentation is the shoot because it is this part
that stores a high percentage of nutrients coming from the soil and from the atmosphere.
Moreover, plants used for FPJ are those that are fast growing such that if you cut the
shoot now, regeneration of a new part will take effect in a few hours. Kamote, Squash,
Kangkong, Alugbati, Banana and Bamboo are some of the plants that could be used and
others with same characteristics and the ones readily available in the area (Diaz et al.,
2011). Kangkong has been chosen because these were abundant in the area where the
study was conducted and proven to give higher yield per hectare.
13
FPJ can be used as a spray at almost any stage of a plant’s growth cycle. It helps
in nutrient absorption, adding vitality and colour to plants and fruits. It must be diluted to
water, 1:500 and is commonly used in conjunction with other inputs (Kalapan Organics,
2013).
with organic fertilizers in tomato, mungbean, cowpea, pepper and eggplant revealed no
significant differences among the different fertilizers in terms of initial height, plant
height at flowering and number of days to harvest on tomato, mungbean, cowpea, pepper
and eggplant (Pagluanan and Anical, 2012). However, statistical analysis showed highly
significant result on the number of days to fruit setting of pepper applied with fermented
plant juice compared to the other treatments, while no significant effect of the different
fertilizers on the number of marketable fruits and weight of marketable fruits on tomato,
mungbean, cowpea and pepper except on eggplant which showed that those plants
applied with fermented plant juice produced the greatest number and heaviest weight of
marketable fruits that resulted in higher yield obtained per hectare (Paluanan and Anical,
2012).
Study result conducted by Mola (2016) on the effect of different plant juices (FPJ)
and weight of marketable and non-marketable fruits per plot and fruit yield/hectare
Plants sprayed with fermented string beans juice significantly had the biggest
lateral fruit diameter (4.74 cm), had the greatest number of marketable fruits produce
14
(147.33/plot), while plants sprayed with fermented camote juice had the least number of
In terms of fruit weight per plot of tomato, plants sprayed with fermented string
beans juice produced the heaviest marketable fruits per plot and fruit yield/ha (19.22
tons).
mankind. Scientists have been researching for technologies naturally available for
applied in the eastern part of the world for the extraction of minerals, enhancement of
microbial consortium that inhabits the soil and the surfaces of all living things inside and
nitrogen fixation, improving soil fertility and as well in the production of plant growth
Ph 3.88
Nitrogen, N 1.86
15
Source: Graduate Thesis (F.V.Aquino, 2018)
have made them likely condition, have made them likely candidates for solving
particularly difficult problems in life science and other fields as well. The responsible
from fossil fuels have increased agricultural production, yet the growing awareness and
concern over their adverse effect on soil productivity and environmental quality cannot
be ignore. The high cost of these products, the difficulties of meeting demand for them,
and their harmful environmental legacy have encouraged scientist to develop alternative
strategies to raise productivity, with microbes playing a central role in these effort
One application is the use of soil microbes as bio inoculants for supplying
nutrients and/or stimulating plant growth. Some rhizospheric microbes are known to
synthesis plant growth promoters and antibiotics, as well as aiding phosphorous uptake.
The last 50 years have seen quick steps made in our appreciation of the diversity of
biology and biotechnology has only quickened the pace of developments (Patil et
16
al.,2014).
inhabits the soil and the surface of all living things inside and out which have the
solubilizes and plant growth promoters (Umiand Sariah, 2006). Without these microbes,
the life will be wretched and melancholic on this lively planet for the survival of human
race.
from invasion by microorganisms with a great potential for causing diseases. They
compete with the pathogens for essential nutrients and for making the environment
nutrients to host plants (Vessey, 2003) and increase the water-holding capacity, making
the plants to have sufficient (or) enough water all the time. It improves the aeration to the
plant root such that exchange of gases takes place effectively and prevents soil erosion.
(MIO), revealed no significant differences among treatment in terms of polar and lateral
diameter of tomato fruits, number and weight of marketable and non-marketable fruits
per plot and fruit yield/ha. However, though no significant results were gathered, plants
sprayed with highest level of IMO registered the longest polar diameter, posted the
biggest lateral fruit diameter, had the greatest number of marketable fruits produced
17
fruits/plot. Likewise, plants applied with 6 tbsp of IMO/liter of water gave the best
Furthermore, plants sprayed with 8 tbsp of IMO/Li of water produced the heaviest
marketable fruits per plot and produced the heaviest marketable fruit yield/ha, while
plants sprayed with 2 tbsp IMO/Li of water registered the heaviest non-marketable fruits
per plot.
18
CHAPTER 2
METHODOLOGY
This chapter presents the methodology of the study. Specifically gives the details
to the methods and procedures and explains how the data will be collected, analyzed and
interpreted.
Materials
Fertilizers:
Inorganic
o 14-14-14
o Calcium nitrate
o Boron
o Magnesium
Organic
o Extreme 300
Pesticides:
Insecticides
o Methomyl
o Ascend
19
o Deltamethrin
Fungicides
o Dithane
o Kumulus df
o Difeconazole
Knapsack sprayers
Plastic trays
Meter tape
Weighing scale
Measuring spoon
Scissors
Kangkong tops
Fish trash
Rice
Molasses
Jar
Clean cloth
Manila paper
Plastic twine
20
Research Methods
The experiment will be laying out in the field in random design. Each application
will be replicated in three (3) times. Treatments will be consisting of twelve (12) plots
with plot size of 5 m x 5 m. Distance between plots will be .80 m and distance between
plants will be 1 m.
21
Experimental Procedure
22
FISH AMINO ACID (FAA)
23
After 14 days, the mixture will be
strained and will place in a clean
bottle.
Indigenous Micro-Organisms
(IMO)
24
The bamboo will be buried in the soil
at a depth equivalent to the
container’s height under a bamboo
grove where there will be a thick
accumulation of decomposing plant
residues to hasten growth of molds.
25
Will be covered with manila paper
and will be secured with rubber band.
It will be stored in a secured place for
seven days to ferment.
Days to flowering (d). The number of days to flowering will be counted which
will starts from the date of sowing and will end when 50% of the population in
Vine length (cm). The average vine length of five random plants per treatment
26
Per cent Fruit Set (%). The number of female flowers produced by each plant
will be counted during flowering. The percentage of fruit set will base on the
number of fruits that will develop over the number of female flowers producing
transplanting to harvesting will be recorded when 80% per cent of the fruits will
Marketable and unmarketable fruits (%). The number of good size and good
fruits shape will be recorded. Too small (0.50 kg below) and deformed fruits will
consider unmarketable fruits and will be recorded. The percentage marketable and
Weight per fruit (cm). Fruit size will be determined by taking the polar and
equatorial diameter of the fruits and classified as: extra small, small, medium and
large.
27
Fruit Acceptability. This will be obtained by selecting random samples for every
treatment using the fruit acceptability rating index. Evaluation of groups will be
Index Taste
1 Bland
2 Slightly Bland
3 Sweet
4 Very Sweet
1 Spongy
2 Less firm
3 Firm
4 Compact
Cost and return analysis. Cost and return analysis will be computed based on
STATISTICAL ANALYSIS
Design.
28
References
MILLER, S.A., F.D. BROOM, T.G. THORP and A.M. BARNETT, 2001. Effects of
Pruning on Vine Architecture, Productivity and Fruit Quality in Kiwi Fruit Scientia
Horticulturae, 91(3), 14 December, 2001, pp. 189-199.
29
PALADA, MC AND CHANG, LC. Suggested cultural practices for bitter gourd,
AVRDC, 20043; (3): 547.
PATIL, S.S. E.M. ADETUTU, J. ROCHOW, J.G. MITCHELL and A.S. BALL.
2014. Sustainable Remediation, Electrochemically Assisted Microbial Dechlorination
of Tetrachloroethene-Contaminated Groundwater. MicrobBiotechnol, Vol. 7, No. 1
54-63. L. Growth and Yield Performance of Different Vegetables Applied with
Organic Fertilizers. Abra State
THAN, THAN NU. Pruning effect on the yield of cucumber varieties AVRDC,
Myanmar 1996; p 51
30
PAGLAUNAN, E.R. AND R.V., ANICAL. Growth and Yield Performance of
Different Vegetables Applied with Different Organic Fertilizers. Abra State Institute
of Science and Technology, Lagangilang, Abra (Philippines)
http://agrisfao,org/agrisearch/search.do?record ID=PH2013000084.2012
ROOFTOP ECOLOGY. Korean Natural Farming DIY agro-input – Fish Amino Acid
(FAA) http://rooftopecology.wordpress.com/2009/10/09/korean-natural-farming-diy-
agro-input-fish-amino-acid-faa/.2009.
31