Scribd Logo
Upload

Welcome to Scribd!

  • 14 views

    Terrific Broth: Product Description

    Uploaded by

    Nabila Putri Sabila
    This document provides information on Terrific Broth medium, including its product description, formula, directions for use, preparation instructions, physical and chemical properties, and microbiological test results. Terrific Broth is a high cell density medium used for cultivating recombinant E. coli strains. It contains yeast extract, tryptone, dipotassium phosphate, and monopotassium phosphate to support growth and maintain the logarithmic phase for protein and plasmid DNA yield. Glycerol is added as the carbon source.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Terrific Broth: Product Description

    Uploaded by

    Nabila Putri Sabila
    14 views2 pages
    This document provides information on Terrific Broth medium, including its product description, formula, directions for use, preparation instructions, physical and chemical properties, and microbiological test results. Terrific Broth is a high cell density medium used for cultivating recombinant E. coli strains. It contains yeast extract, tryptone, dipotassium phosphate, and monopotassium phosphate to support growth and maintain the logarithmic phase for protein and plasmid DNA yield. Glycerol is added as the carbon source.

    Original Title

    82111A

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides information on Terrific Broth medium, including its product description, formula, directions for use, preparation instructions, physical and chemical properties, and microbiological test results. Terrific Broth is a high cell density medium used for cultivating recombinant E. coli strains. It contains yeast extract, tryptone, dipotassium phosphate, and monopotassium phosphate to support growth and maintain the logarithmic phase for protein and plasmid DNA yield. Glycerol is added as the carbon source.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    14 views2 pages

    Terrific Broth: Product Description

    Uploaded by

    Nabila Putri Sabila
    This document provides information on Terrific Broth medium, including its product description, formula, directions for use, preparation instructions, physical and chemical properties, and microbiological test results. Terrific Broth is a high cell density medium used for cultivating recombinant E. coli strains. It contains yeast extract, tryptone, dipotassium phosphate, and monopotassium phosphate to support growth and maintain the logarithmic phase for protein and plasmid DNA yield. Glycerol is added as the carbon source.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    FT-82111A

    Terrific Broth
    Medium used with glycerol for the cultivation of recombinant strains of E.coli

    Product Description

    Name : Terrific Broth


    Catalog Number : 82111A, 500g
    82111C, 5 Kg
    82111D, 10 Kg

    Storage 20-25°C. Once opened keep powdered medium closed to avoid hydration.

    FORMULA:
    Yeast Extract: 24.0g/L Dipotassium Phosphate: 12.54g/L
    Tryptone : 12.0g/L Monopotassium Phosphate: 2.31g/L

    The pH after preparing the medium and at room temperature: 7.2 ± 0.2

    Directions for use


    Guidelines for use :
    TERRIFIC BROTH is a medium which supports a high cell density and, in the case is formulated for optimum growth
    of E.coli, maintains growth in the logarithmic phase for a long time. As a result it yields a greater number of
    recombinant proteins and plasmic DNA. In some circumstances it substitutes LB Broth (Lennox) used in genetic
    studies. Tryptone provide nitrogen, vitamins, minerals and amino acids essential for growth Yeast extract is source of
    vitamins, particularly the B-group. Potassium phosphates act as a buffer system to prevent cell death. The source of
    carbohydrates and carbon is glycerol that is not fermented to acetic acid as glucose and does not lead to confusing
    results.

    Preparation :
    Suspend 50.8 grams of the medium in 900 ml of distilled water .Mix well.
    Add 4 ml of glycerol and adjust to a final volume of 1000 ml. and dissolve by heating with frequent agitation.
    Boil for one minute until complete dissolution.
    Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.
    The prepared medium should be stored at 2-8°C.
    The color of the prepared medium is amber.
    The dehydrated medium should be homogeneous, free-flowing and beige in color.
    If there are any physical changes, discard the medium.

    P.1
    FT-82111A

    Physical and Chemical Test :


    Appearance: fine powder
    Solubility(at 3.5%) clear, amber, without rests
    Color: beige
    Color of the prepared medium: amber

    Microbiological Test :
    The following results were obtained in the performance of the medium from type cultures after incubation at a
    temperature of 35 ± 2°C and observed after 18-24 hours.

    Microorganisms Growth
    Escherichia coli ATCC 23724 Good
    Escherichia coli ATCC 33694 Good
    Escherichia coli ATCC 33849 Good
    Escherichia coli ATCC 53868 Good

    Literature :
    Joseph Sambrook, David W .Russell. The condensed protocols from molecular cloning: a laboratory manual.

    Ordering information
    Catalog size quantities and prices may be found at http://www.interchim.com.
    Please inquire for higher quantities (availability, shipment conditions).

    For any information, please ask : Uptima / Interchim; Hotline : +33(0)4 70 03 73 06


    Order on-line or Contact your local distributor
    Disclaimer : Materials from Uptima are sold for research use only, and are not intended for food, drug, household, or cosmetic uses.
    Uptima is not liable for any damage resulting from handling or contact with this product.

    P.2

    You might also like