SHOOL OF MEDICINE
DEPARTMENT OF PHYSIOLOGY
Biomedical Engineering Unit
BME 304 - BIOMATERIALS
BBME – Third Year
Prepared by: Eng. Pinda. O
Lecture Two MSc in Mechanical Engineering
MSc in Biomedical Engineering
Salient Properties of Biomaterials
Mechanical Properties
Strength Biological Properties
Elastic modulus Nontoxicity
Fracture roughness Cytotoxicity
Biological Hardness Osseointegration
Resistant to wear Non-combustible
Biomaterial
Mechanical &corrosion
BIOMATERIALS
Physical
Physical Properties Chemical Properties
Microstructure Chemical structure
Chemical Texture Functional groups
Density Hydrophilic/hydrophobic
Porosity Degradation
Charge
Impact on human tissues pH
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Salient Properties of Biomaterials
A. Biological Properties
Refers to the properties which favor
Biological interaction of biomaterials to tissue /
Biomaterial
organs otherwise material will be rejected
Mechanical
through different reactions.
Physical
Biocompatibility Biodegradable
Chemical Bio-inert Bioresorbability
Toxicity Osteoconductivity
Bioactivity Osteoinductivity
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Definitions of terms
Biocompatibility: Generally refers to the response of biological
organism the presence of biomaterial, not vice versa (Rejection)
→ The ability of a material to perform its desired function with an
appropriate host response in a specific application (D.F. Williams, the
Williams dictionary of biomaterial, 1999)
Appropriate host response → Biomaterial should be;
Non - Blood clotting, Non - Thrombogenic
Non - Bacterial colonization Non- Toxigenic
Non – Carcinogenic
Non - Abnormal heating
Non- immunogenic
Non - Inflammatory
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Bio-inert
The ability of biomaterial to remain unchanged by a biological
organism and to not elicit biological activity
Bioactive
The ability of biomaterial to elicit or modulate a favorable response
(activity) from any party of biological organism such as interaction,
adhesion etc
Biodegradability
The ability of biomaterial to be broken down or decomposed by
biological organism
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Bioresorbability
The ability of biomaterial to be gradually resorbed or dissolved by
cellular and/or metabolic process
Osteoconductivity
The ability of biomaterial to passively allow the growth of pre-
existing bone tissue onto its surface and/or into pores
Osteoinductivity
The ability of biomaterial to stimulate new bone formation
(osteogenesis). Often evaluated by implantation in an ectopic site,
such as muscles
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B. Mechanical Properties
Biomaterials are subjected to several force when they are in
actual application.
→ So their mechanical properties should withstand the effect
of the applied forces (stress, strain, shear)
→ Mechanical properties of materials depend on
microstructure and can be modified through processing
techniques which cause rearranging of microstructure or
combining more than one materials together (Composite)
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Mechanical properties mostly refers to;
Tensile strength, Compression strength, yield strength, elastic
modulus, corrosion, wear, creep, and hardness etc.
The strength of material can be analyzed by stress- strain curve.
A: Limit of proportionality, elastic limit
B: Yield point (Upper yield point)
C: Lower yield point
D: Ultimate stress
E: Fracture stress
𝑆𝑡𝑟𝑒𝑠𝑠(𝜎)
𝑌𝑜𝑢𝑛𝑔 𝑀𝑜𝑑𝑢𝑙𝑢𝑠 𝐸 = 𝑆𝑡𝑎𝑖𝑛 (𝜀)
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Definitions of terms
Elasticity
Is the property of the material which enables the material to
recover to its original form after the external force is removed.
Plasticity
Material undergo permanent deformation without fracture even
after the external force is removed
Creep strength
Ability of materials withstand slowly deformation over long period
under (high) stress.
Young’s modulus of elasticity (E)
By Hooke's law, within the proportional limit, stress = E × strain.
𝜎 𝐹𝐿
𝐸 = = 𝑜 (Pa)
𝜀 𝐴𝑜 𝑒
Where;
E → Young’s modulus of elasticity
F → Applied force
𝐿𝑜 → Original length
𝐴𝑜 → Original area
e → Elongation
Hooke’s Law, states that;
Within the proportional limit (straight line between zero and A),
stress is proportionate to strain.
C. Physical Properties
This refers to size, shape, surface texture (roughness & topography),
porosity and wettability which plays a decisive role in the adhesion,
growth and differentiation of cells.
Adhesion and growth of cells is optimum on materials with moderate
wettability
D. Chemical Properties
This refers to chemical behavior due to material composition and
structure at atomic and molecular levels.
→ Types of atoms, bonding between atoms, crystalline structure.
The properties includes;
Reactivity, electronegativity, biodegradability, absorption,
degradation, corrosion resistance
Applications of Biomaterials
a) Medical implants: Replacement of diseased or damaged part
Heart valves, Artificial joints
Stents and grafts
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Implants cont…
Hearing loss implants Dental implants and
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b) Assist in healing
Promote tissue formation, preserve
volume and shape, and shorten the
time of wound healing
Includes:
Sutures
Clips and staples for wound closure
Dissolvable dressings
Bone plates and screws
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c) Assist in drugs delivery
Includes:
Catheters
Bags
Syringes
tubes,
infusion pumps
IV Cannula
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d) Assist in Diagnosis and Treatment
Includes:
Probes,
Catheters
Encapsulations
Drains
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e) Improve and correct f) Correct cosmetic problem
function abnormality
Includes: Includes:
Cardiac pacemaker Augmentation
mammoplasty
Intraocular lens
Chin Augmentation
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Summary
Applications
Biomaterials
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Biomaterials classes with
properties comparison
and effective area of
applications (Wagner
2020)
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Review on Salient Properties of Biomaterials
Hereunder are properties for any material to qualify as a
biomaterial.
Biocompatibility: Non-toxic, non-carcinogenic, non-allergenic,
etc.
Adequate mechanical properties
Essential physical and chemical properties
Stable durability for intended period i.e., Hrs to Yrs
Easy to process with the available techniques
Sterilizable with current facilities without any difficulty
Cost-effective and accessible
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Concept of Biocompatibility
When a Biomaterial is placed in the human body different
molecular interactions take place at the material surface
Types of interactions are Thus, the surface texture of
affected by the surface biomaterial governs the
properties of the material such interaction biological reactions
which mainly are;
Surface chemistry
Protein adsorption
Wettability and Cell adhesion
Surface roughness Cell growth and
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Therefore, the interaction depends on the type of biomaterials and
its surface, e.g consider the knee joint bellow;
Noted that;
Proteins (red) adsorb differently to different materials and are
depicted as elongated in metal and globular on polymer.
Cell (blue) interact with the material via adsorbed proteins
dictates how the cell will respond (adhere, proliferate,
differentiate, etc.)
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Interaction occurs through four stages;
Water-surface interaction, Cell attachment and spreading, and
Protein adsorption, Proliferation.
Stages of Biomaterial interaction
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a) Water surface interaction
It is an initial and first stage: Water molecule from human body is
adsorbed onto biomaterial surface.
Water can either spread or form droplet on the surface, this is
measured by contact angle (𝜃)
.
Hydrophilic
𝜃 < 90°
Hydrophobic
𝜃 > 90°
b) Protein adsorption
2𝑛𝑑 stage: Occurs in seconds to minutes, a monolayer of protein is
adsorbs to the most surfaces
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c) Cell – biomaterial interactions
3𝑟𝑑 stage: The cell is attached to the biomaterial surface and is
influenced by the adsorbed protein layer as well as surface
topography.
The cells primarily see the adsorbed protein layer rather than
the biomaterial surface itself.
d) Cell proliferation
4th stage: Is the process of increase in the number of cells which
occurs as a result of regulated cell growth and cell division.
The cells increase exponentially resulting on rapid tissue growth
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It is influenced by both microscale roughness and wettability
The process is balanced by cell division and cell differentiation or
cell death, which maintains an appropriate number of cells in the
body.
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Assessment of Biocompatibility of Biomaterials
Biocompatibility of biomaterials can be assessed and tested by
the following criteria
Cytotoxicity (Tissue culture)
Genotoxicity
Hemocompatibility
Mutagenicity,
Carcinogenicity and
Immunogenicity
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Toxicity: Refers to ability of biomaterial to damage a biological
system by chemical means.
Cytotoxicity: Refers to damage to individual cells, for example in
cell cultures
Immunogenicity: Referred to the ability of a substance to provoke
an immune response or the degree to which it provokes a
response
Genotoxicity: Describes an alteration of the base pair sequence of
the genome DNA → Cells possess numerous mechanisms to repair
genotoxic damages.
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Carcinogenicity: Is the alterations in the DNA that caused a cell to
grow and divide inappropriately → Alterations of DNA promoted
the generation of malignant tumors.
Testing of Biomaterials
Based on specific requirements to the area of application of
biomaterials different testing techniques have to be carried out to
assess the tissue response and the clinical relevant performance of
newly developed biomaterials.
Biocompatibility tests Surface topography test
Mechanical strength tests
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ASSIGNMENT
Q1. With the aid of sketch or block diagram/flow chart/process
elaborate at least three tests carried out on biomaterials to
evaluate its salient properties in the following categories;
Biocompatibility
Mechanical strength
Surface topography
Assessment: Presentation 20 minutes, Discussion 10 minutes
Presentation dates: 05th, 09th, 12th and 16st December, 2022
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