1100 Series
1100 Series
LC Devices
Connecting the Hardware and Triggering Data
Acquisition
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Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .v
About This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition iii
Preface
Related Documentation
In addition to this guide, Thermo Fisher Scientific provides Help available from within the
Xcalibur software.
CAUTION Highlights electric shock related hazards to human beings. Each electric shock
notice is accompanied by the international High Voltage symbol.
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition v
Safety Precautions
To connect the Agilent 1100 Series LC stack to a Thermo Scientific mass spectrometer, you
must install an external contact board in one of the Agilent modules and a LAN interface
board in a second Agilent module. To install a board, you must remove the cover from the
optional board slot. Figure 2 on page 6 shows the location of the slot on the back panel of the
detector.
Before you remove the slot cover from an Agilent 1100 Series module, do the following:
• Turn the power switch to the Off position and unplug the power cable.
CAUTION To avoid an electric shock, always turn off an Agilent module and unplug
it from line power before you remove its cover.
• Refer to the Reference Manual for the module for additional safety information and for
information on preventing ESD damage caused by an electrical discharge.
Contacting Us
There are several ways to contact Thermo Fisher Scientific.
Phone 800-685-9535
Fax 561-688-8736
E-mail [email protected]
Knowledge base www.thermokb.com
Find software updates and utilities to download at www.mssupport.thermo.com.
vi Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
Phone 800-532-4752
Fax 561-688-8731
Web site www.thermo.com/finnigan
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition vii
1. Make sure that the Agilent 1100 Series modules are connected by CAN communication
cables, that all the modules are turned on, and that the Agilent 1100 Control Module is
connected.
Refer to the Agilent 1100 Series LC reference manuals for instructions on how to connect
the CAN cables.
2. Press the Esc key on the Control Module until System appears in the upper-left corner of
the LCD display.
3. Press the F4 key on the Control Module to access the Records LCD display.
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 1
Time Ready
Records
4. Check the firmware versions of the Agilent 1100 Series modules. Refer to Table 1 for the
firmware versions supported by the Xcalibur 2.0. and higher data system.
2 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
Contents
• Ethernet Communication Kit
• Installing a Network Card in an Agilent Module
• Connecting the Ethernet Communication Cables
• Installing the External Contact Interface Board
• Connecting the Trigger Cable
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 3
Tip Vendors label network cards with a unique MAC address, which is a 12-digit
hexadecimal number. The first portion of the MAC address identifies the vendor. The
second portion of the MAC address is a unique serial number. For example, a typical mark
on a network card is AD: 0030C1C49982, in which 0030C1C49982 is the stack MAC
address. The first portion, 0030C1, identifies the vendor as Hewlett-Packard. The MAC
address of the HP JetDirect 400N network card is located on the back of the card.
Because you need the MAC address when you configure the Agilent stack, record the
network card MAC address before you install the card.
Agilent 1100 Series LC systems with JetDirect® network cards must have revision 2
mainboards to function properly with LAN communications. Table 3 shows the required
revision level for each module. The serial number listed in the table and all serial numbers
after that number are supported.
Table 3. Supported serial number
Module P/N (Mainboard) Serial Numbers for Supported Version *
G1310A G1311-65520 DE64300355 (made in Germany)
Isocratic pump US64400233 (made in U.S.A.)
G1311A G1311-65520 DE64301137 (made in Germany)
Quaternary pump US64401134 (made in U.S.A.)
G1312A G1312-65520 DE64300703 (made in Germany)
Binary pump US64400425 (made in U.S.A.)
G1313A G1313-65520 DE64302092 (made in Germany)
ALS autosampler US64400886 (made in U.S.A.)
G1314A G1314-65520 JP64201926 (made in Japan)
Variable wavelength detector
G1315A G131-65520 DE64301532 (made in Germany
Diode array detector US64400333 (made in U.S.A.)
*
All serial numbers above the listed number in numeric order are supported
4 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
1. Make sure the module (usually the detector) is turned off and unplugged from line
power. Figure 1 shows the location of the power switches.
When the power switch of a module is in the On position, the LED on the switch is
green. When you turn the power switch of a module to the Off position, the LED
turns off.
If your LC stack contains a detector (for example, diode-array detector, variable
wavelength detector, multiple wavelength detector), install the network interface card in
the detector. If your LC stack does not contain a detector, install the network card in any
module with an open slot.
CAUTION To avoid electric shock, unplug the power cable that connects the Agilent
module to line power. After you turn the power switch to the Off position, the power
supply continues to draw current.
Tip You can install an Agilent LAN interface board (model G1369A) instead of a
JetDirect 400N network card.
Solvent cabinet
1100 Series
Binary pump
Power switch
1100 Series
F8
F7
F6
F1 F2 F3 F4 F5 i
7 8 9
Esc ◄
◄ Enter
4 5 6
1 2 3
0 . -
Autosampler
Power switch
1100 Series
Column
compartment
Power switch
1100 Series
Detector
Power switch
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 5
2. To prevent damage to the interface board, ensure that you are using ESD protection. For
more information on preventing damage caused by an electric discharge, refer to the
Reference Manual for the Agilent module.
3. To remove the cover plate from the slot where you plan to install the network interface
card, loosen the two screws that fasten the plate to the chassis of the module. Figure 2
shows the location of the cover plate on the back panel of an Agilent 1100 Series detector.
Figure 2. Back panel of an Agilent 1100 Series detector
Cover plate
4. Record the MAC address (12-digit hexadecimal number) located on the back of the HP
JetDirect 400N network card. You will need this number to complete the configuration
of the Agilent stack. See Figure 6 on page 12.
5. Insert the HP JetDirect 400N network card into the slot, and then tighten the two screws
that fasten the PCB to the chassis of the module.
6 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
1. Connect a category 5, shielded Ethernet cable from the JetDirect 400N network card to
the Ethernet switch.
CAUTION To comply with safety and EMC regulations, you must use category 5,
shielded Ethernet cables to make the Ethernet connections for your instrument.
2. Connect a category 5, shielded Ethernet cable from the Ethernet switch to the Ethernet
card in the computer dedicated to the LC system (typically network interface card
number 3).
Figure 3. Ethernet connections
JET DIRECT 400N
100 (RJ-45)
100 (RJ-45)
PACKARD
HEWLETT
BNC
BNC
JetDirect 400N card
Computer
(installed in one of
Optiplex GX520
the Agilent modules)
(subject to change)
Not drawn to scale
USB
ENET
Ethernet Switch
Cat5 shielded
Ethernet cables
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 7
1. If you have not already done so, turn off the autosampler and unplug it from line power.
CAUTION To avoid electric shock, unplug the power cable that connects the Agilent
module to line power. After you turn the power switch to the Off position, the power
supply continues to draw current.
2. To prevent damage to the interface board, ensure that you are using ESD protection. For
more information on preventing damage caused by an electric discharge, refer to the
Reference Manual for the Agilent module.
3. To remove the cover plate from the slot where you plan to install the external contact
interface board, loosen the two screws that fasten the plate to the chassis of the
autosampler.
4. Insert the external contact interface board (see Table 2 for the part number) into the slot,
and then tighten the two screws to fasten the board to the chassis of the autosampler. See
Figure 4.
Figure 4. View of the external contact interface board installed in an Agilent 1100 Series
autosampler
Screws for fastening
the external contact interface board
1 8
8 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
Y To connect the trigger cable to the Agilent autosampler and the MS detector
1. If you have not already done so, install the external contact interface board as described in
“Installing the External Contact Interface Board.”
2. Connect the 2-pin connector to the I/O panel of the MS detector, such that the
white wire is connected to the Start In+ pin and the brown wire is connected to the
Start In– pin.
3. Connect the DB15 connector to the RELAY CONTACTS receptacle located on the
external contact interface board of the Agilent 1100 Series autosampler. See Figure 5.
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 9
START IN
Connection to autosampler
LXQ or LTQ RELAY CONTACTS
MS detector
(Right-side panel)
White wire
Brown wire
START IN
+-
START IN +
LCQ Series Peripheral –
(5V Max)
(Left-side
panel)
Surveyor MSQ
(Rear panel) Start In +
Start In -
Trigger Cable
Not drawn to scale
10 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
1. Choose Start > All Programs > Xcalibur > Instrument Configuration.
The Instrument Configuration dialog box appears.
2. Scroll through the Available Devices area and double-click the Agilent 1100 AS button.
The Agilent 1100 AS button is copied to the Configured Devices area and is displayed as
a Configured Devices button.
3. Double-click the Agilent 1100 AS button in the Configured Devices area.
The Agilent 1100 Autosampler dialog box appears.
4. Click the General tab.
The General page appears. See Figure 6
5. In the General page, make the appropriate selections:
• Select the Contact Board Installed check box.
Note If you do not select the Contact Board Installed check box, the Timed
Events page does not appear in the Instrument Setup view for the autosampler.
• In the Stack MAC address box, type the media access control address for your
Agilent LC stack.
The stack MAC address is a unique identification for each network card. The
manufacturer usually stamps it on the network card.
Note The TCP/IP settings are shared by all Agilent 100 LC modules in the stack.
Changing the value of a setting for one module in the Instrument Configuration
dialog box changes the value of that setting for all modules in the stack.
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 11
• In the Stack IP Address box, type the IP address for your Agilent 1100 LC stack.
Leave the value set to the default or contact your network administrator for the IP
address.
The default stack IP address is 172-16-0-102.
• In the Sub-mask box, type the subnet mask (address mask). Leave the subnet mask set
to its default value, or contact your network administrator for the subnet mask.
The default subnet mask value is 255-255-255-0.
• In the Bootp Server box, type the IP address for the network card in your PC that is
responsible for assigning the stack IP address for your Agilent 1100 LC system. Leave
the value set to the default or contact your network administrator for the BOOTP
server IP address.
The default IP address for the network card is 172-16-0-101.
6. Click OK to save the settings and close the dialog box.
Figure 6. Agilent1100 Autosampler dialog box
12 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
Note Configure the Agilent 1100 Series autosampler before performing the following
procedure. Refer to Chapter 3, “Configuring the Autosampler for Contact Closure,”
6. Make the appropriate entries and selections for the rest of the instrument method.
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 13
14 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
Y To turn off the solvent tracking feature on the Agilent 1100 pump
F8
F7
F6
F1 F2 F3 F4 F5 i
7 8 9
Esc ◄
◄ Enter
4 5 6
1 2 3
0 . -
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 15
16 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
1. Order the appropriate upgrade parts from your local office for Thermo Electron San Jose
products:
• If the HP 1100 is not interfaced to an MS detector as an Xcalibur-controlled GPIB
inlet device, order the JetDirect Ethernet Control kit, which contains the parts listed
in Table 4.
• If the HP 1100 is interfaced to an MS detector as an Xcalibur-controlled GPIB inlet
device, order the parts listed in Table 5.
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 17
18 Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific
Index
A J
Agilent 1100 Series LC JetDirect boards, supported 4
configuring autosampler for contact closure 11
external contact interface PCB, installing 8
solvent tracking feature, deactivating 15
M
supported mainboards for JetDirect cards 4 MAC address 4
Agilent LAN interface board (G1369A) 5 mainboards supported for JetDirect Cards 4
C S
configuring the Agilent 1100 Series Autosampler 11 safety precautions vi
contact board 11 slot cover, removing from an Agilent module vi
contact closure, configuring the Agilent Autosampler for 11 solvent tracking feature for Agilent pumps 15
Contacting vi stack IP address 12
stack MAC address 4, 12
D
data acquisition, triggering 13
X
deactivating the solvent tracking feature for Agilent 1100 LC Xcalibur data system
pumps 15 Instrument Configuration program, configuring the
Agilent 1100 series autosampler for contact closure 11
Instrument Setup view 13
E
Electric shock safety precaution 5, 8
ESD precaution vi, 6, 8
Ethernet cable connections 7
Ethernet card for upgrading HP 1100 LC 17
F
firmware for Agilent LC 1
H
HP 1100 Series LC, upgrading to Ethernet interface 17
I
instrument methods, triggering data acquisition 13
Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 19