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    Death Penalty Essay Titles

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    1. Two E. coli bacterial suspensions were prepared, one labeled +pGLO to which the pGLO plasmid was added, and one labeled pGLO without the plasmid. 2. The suspensions were incubated on ice, then heat shocked and returned to ice to facilitate uptake of the plasmid. 3. The bacteria were spread on nutrient agar plates with and without ampicillin to select for transformed cells. 4. One plate also contained the araC protein to induce expression of the pGLO plasmid and visualize glowing colonies. 5. The transformation efficiency was calculated based on the number of glowing colonies formed.

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    Death Penalty Essay Titles

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    ezknbk5h
    56 views4 pages
    1. Two E. coli bacterial suspensions were prepared, one labeled +pGLO to which the pGLO plasmid was added, and one labeled pGLO without the plasmid. 2. The suspensions were incubated on ice, then heat shocked and returned to ice to facilitate uptake of the plasmid. 3. The bacteria were spread on nutrient agar plates with and without ampicillin to select for transformed cells. 4. One plate also contained the araC protein to induce expression of the pGLO plasmid and visualize glowing colonies. 5. The transformation efficiency was calculated based on the number of glowing colonies formed.

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    1. Two E. coli bacterial suspensions were prepared, one labeled +pGLO to which the pGLO plasmid was added, and one labeled pGLO without the plasmid. 2. The suspensions were incubated on ice, then heat shocked and returned to ice to facilitate uptake of the plasmid. 3. The bacteria were spread on nutrient agar plates with and without ampicillin to select for transformed cells. 4. One plate also contained the araC protein to induce expression of the pGLO plasmid and visualize glowing colonies. 5. The transformation efficiency was calculated based on the number of glowing colonies formed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    56 views4 pages

    Death Penalty Essay Titles

    Uploaded by

    ezknbk5h
    1. Two E. coli bacterial suspensions were prepared, one labeled +pGLO to which the pGLO plasmid was added, and one labeled pGLO without the plasmid. 2. The suspensions were incubated on ice, then heat shocked and returned to ice to facilitate uptake of the plasmid. 3. The bacteria were spread on nutrient agar plates with and without ampicillin to select for transformed cells. 4. One plate also contained the araC protein to induce expression of the pGLO plasmid and visualize glowing colonies. 5. The transformation efficiency was calculated based on the number of glowing colonies formed.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Death Penalty Essay Titles

    Writing an essay on the topic of "Death Penalty Essay Titles" can present a unique set of challenges.
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    Death Penalty Essay Titles Death Penalty Essay Titles
    Similarities Between The Wanderer And The Dream Of The
    Rood
    Comitatus in The Wanderer and The Dream of the Rood The Wanderer and The Dream
    of the Rood are two Old English poems that demonstrate the link between lord and thane.
    This bond, also known as the comitatus, is highlighted with imagery to effectively
    portray the physical intimacy involved. The idea that everything is fleeting is
    emphasized to show the significance of the comitatus. Furthermore, the beauty of the
    relationship is shown by contrasting the shame that the Wandererfeels at the end of the
    poem to the honour and glory that is thrown upon the crossafter it willingly suffers along
    with Christ. In all, the ideals of the comitatus during the Medieval Times are clearly
    advocated through the illustration of the physical intimacy, shame and honour
    involved in the two poems. To start off, the two poems of interest both share the
    central idea that everything in life is fleeting. The Wanderer is a lament on the lost
    ways of life. The Wanderer thinks back to the times he was free from exile. He
    nostalgically compares his life then to the one he has now and comes to realize how
    quickly the time has gone by. From lines 73 to 77, it says The wise man must realize
    how ghastly it will be when all the wealth of this world stands waste, as now here and
    there throughout this middle earth walls stand blasted by the wind, beaten by frost, the
    buildings crumpling. There is a lack of hope in this passage. The idea that things that
    seem concrete are very temporary is emphasized when
    Mexican Revolution
    Assignment 1: Major Causes of the revolution in Mexico.
    Based on John Tutino, From Insurrection to Revolution in Mexico

    The Mexican Revolution (1910 1917) was caused by a variety of factors. It is


    impossible to place the blame on one single event or person because of the complexity
    of the Mexican people. One thing is for sure, if people are deprived of food and water,
    they will find a way to obtain enough to survive. History proves that in desperate times
    people will take matters into their own hands. When a countries leadership wavers, and
    conditions become poor enough that people are starving, they will respond negatively. It
    seems that any time the Mexicans lose the ability to grow their own maize they become
    discontent. ... Show more content on Helpwriting.net ...
    Diaz had done well at expanding the economic system especially by producing a system
    of Mexican Railroads. He is also given credit with the economic growth in the Southern
    Regions. Mexican land owners were losing land for owing money. For example.
    In Central Mexico, a heavily indebted Mariano Riva Palacio lost in 1870 the estates he
    had worked to acquire since the 1830 s, and that had provided the economic base at
    Chalco that underlay his political dominance of the state of Mexico for years.
    Tutino also discussed how interaction with the French could also lead to loss of land.
    Large chunks of land were broken up under Diaz, in order to redistribute lands in order
    to yield more productive areas to generate commerce. Crop failures in 1907 were
    another cause; the people could not afford to import American Maize, and were
    unable to grow their own crops for subsistence. All of these separate but different
    events led to the people of Mexico revolting, and brought a significant amount of
    change to the landscape of Mexico ever since. In the southern areas were uprisings
    were held to a minimum, people were still able to feed themselves and their children.
    The Elite were still making money and able to afford the near slave labor wages by
    giving the workers who migrated advances on their pay in the off seasons, for a promise
    to go to work at a later date. This system was used in the North, but ultimately failed
    E. Coli Bacterial Suspension
    In this experimental part of the lab, two bacterial suspensions were made by adding
    250µl of a transformation solution of calcium chloride, as well as adding and
    completely dispersing two large colonies of E. coli of approximately 2 mm in diameter,
    that were collected from the starter plate. Each suspension was labeled, one as
    +pGLO, and the other as pGLO. 10µl of p GLO plasmid was added to the +p GLO
    suspension. Both suspensions were incubated for a period of 10 minutes on ice.
    The bacterial suspensions were removed from the ice and then heat shocked in a water
    bath at 42 °C for exactly 50 seconds, both suspensions were quickly placed back on the
    ice for an incubation period of 2 minutes.
    The LB nutrient broth was added to both bacterial suspensions and incubated for 15
    minutes in a water bath at 37 °C.
    Bacterial Cells Spreading
    Four LB nutrient agar plates were obtained and labeled. Two of the plates were the
    transformations plates appropriate labeled as +pGLO/LB/amp ... Show more content on
    Helpwriting.net ...
    The results obtained in the bacterial culture plates were the following: In Plate # I, the
    bacteria grew as a lawn of bacteria, making it uncountable. In plate # II without pGLO
    plasmid, there were no bacteria, all of them died in the presence of ampicillin. In plate
    # II with pGLO plasmid, the bacterial culture lived and grew. In plate # III with pGLO
    plasmid and araC protein, the bacterial culture grew and glowed. The transformation
    efficiency is the number of colonies on LB/amp/ara plate divided by the amount of DNA
    on the plate. Figure 1 shows the culture plates under visible light, figure 2 shows the
    culture plates under ultraviolet light, table 1 shows the characteristics of the culture
    plates obtained after the experiment, and Table 2 shows the calculations done for finding
    the transformation

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