Practice Questions - Proteins

Name: ___________________________ CHEM 133 YB/YC/EDSCI 19
STUDY QUESTIONS
Provide the best answer for the following questions.
1. The chirality of an amino acid results from the fact that its alpha carbon:
A) is a carboxylic acid
B) is bonded to four different chemical groups
C) is symmetric
D) is in the L absolute configuration in naturally occurring proteins
E) has no net charge
2. The side chains of nonpolar amino acids are best categorized as:
A) hydrophobic
B) positively charged
C) negatively charged
D) uncharged
E) hydrophilic
3. In a highly basic solution, pH = 13, the dominant form of glycine is:
A) NH2-CH2-COOH
B) NH3+-CH2-COOH
C) NH2-CH2-COO
D) NH3+-CH2-COO
E) NH2-CH3+-COO
4. The peptide alanylglutamylglycylalanylleucine has:
A) four peptide bonds
B) two free amino groups
C) a disulfide bridge
D) no free carboxyl group
E) five peptide bonds
5. What functional groups are present on this molecule?
A) hydroxyl and carboxylic acid

B) hydroxyl and aldehyde
C) hydroxyl and ketone
D) ether and aldehyde
E) hydroxyl and ester
6. Which of the following amino acids are aromatic?
A) Phenylalanine, alanine, and glycine
B) Phenylalanine, histidine, and tyrosine
C) Histidine, tyrosine, and tryptophan
D) Tyrosine, tryptophan, and cysteine
E) Phenylalanine, tyrosine, and tryptophan
7. To precipitate proteins from solution, technicians often adjust the pH to the isoelectric point of
the protein. The purpose in doing this is to:
A) Prevent hydrolysis
B) Decrease solubility of protein molecules
C) Maintain disulfide linkages
D) Promote repulsion by protein molecules having like charges
E) Increase hydration of acid and amine groups
8. Which pair of amino acids absorbs the most UV light at 280 nm?
A) Thr & His
B) Trp & Tyr
C) Cys & Asp
D) Phe & Pro
E) None of the above
9. The isoelectric point of an amino acid is defined as
A) the pH where the molecule carries no net electric charge.
B) the pH where the carboxyl group is uncharged.
C) the pH where the amino group is uncharged.
D) the pH of maximum electrolytic mobility.
E) -log10(pKi + pKj)
10. Identify the element found in a protein that is not present in a carbohydrate
A) Carbon
B) Oxygen
C) Hydrogen
D) Nitrogen
11) What monomer of a protein is being shown?
A) monosaccharide
B) glycerol and fatty acids
C) amino acid
D) nucleotide
12) What are the bonds that connect amino acids in a protein called?
A) peptide bonds
B) ester bonds
C) glycosidic bonds
D) phosphodiester bonds
13)This protein is used to identify disease causing organisms like bacteria when they enter the body.
A) hemoglobin
B) insulin
C) keratin
D) antibodies
14) Draw the structure of the tripeptide Phe-Glu-Lys as it would appear at pH = 7.

15) The following is the amino acid sequence of bradykinin, a peptide released by certain organisms
in response to wasp stings:
Arg – Pro – Pro – Gly – Phe – Ser – Pro – Phe – Arg
What amino acids or peptides are produced when bradykinin is treated with each of the
following reagents?
a) with Carboxypeptidase c) with Trypsin
b) with Chymotrypsin d) with DNFB
16) Identify each of the following observations about protein structure as being aspects of the 1o, 2o,
3o, or 4o structure
______ A. Amino acids 14–38 in the sequence are arranged in α–helix pattern.
_______ B. When the protein was enzymatically broken apart, one of the fragments was found to be
Leu-Tyr-Gly-Ala-Lys.
_______ C. The entire molecule is globular 1.3 times as long in one dimension as its diameter.
_______ D. Detergent was added to a water solution of the protein. The molecular weight study
before the addition indicated a single protein with a molecular weight of 150,000. After
the addition, two proteins were present with molecular weights 25,000 and 50,000.
17) Describe all the different levels of the hierarchy of protein structure (from primary through to
quaternary).
18) Describe the structural and functional similarities and differences between myoglobin and
hemoglobin.

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Name: ___________________________ CHEM 133 YB/YC/EDSCI 19

Provide the best answer for the following questions.

A) hydroxyl and carboxylic acid

14) Draw the structure of the tripeptide Phe-Glu-Lys as it would appear at pH = 7.

a) with Carboxypeptidase c) with Trypsin

b) with Chymotrypsin d) with DNFB

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