DR-200Bc User Manual
DR-200Bc User Manual
DR-200Bc User Manual
Microplate Reader
Instruction Manual
English
Table of Contents
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Section 1 Important Safety Instruction
Dangerous or wrong using method may cause electric injuries, burns, fire or other disaster.
Basic safety protection including all the followings should be always obeyed.
When the instruments are used near children, disabled people or samples, rigorous monitoring
should be performed.
1.1 Read the followings before using the instrument
1) Check correspondence of voltage equipment and supply voltage.
2) Connect with network source: Put the instrument plug in the socket which has a grounded power
cord.
3) Take the plug out after finishing operating the instrument.Do not place the instrument in a difficult
position to operate the disconnecting device.
4) You mustn’t put the instrument in or near liquid. If the instrument is wet, please take the plug out
before touch it.
5) Please do not leave the instrument alone when it is working.
6) The instrument can only be used for tests according to the manual.
7) Do not use spare parts which are not provided or suggested by the manufacturer.
8) If the instrument is in abnormal working position or damaged, do not operate it.
9) Do not make instrument and lines touch surfaces which are too hot.
10) Do not block vents. Make vents away from soft cloth, fur, lint etc.
11) Do not put anything on the instrument.
12) Unless special requirements of the manual, you can not put anything into opening, pipe or seam of
the instrument.
13) Do not use instrument in the place where exits aerosol, droplet or oxygen is controlled.
14) Do not use instrument outdoors.
15) When dealing with hazardous substances, it should be handled by professionally trained
personnel.
16) Keep far away from electromagnetic interference sources.
17) Avoid direct exposure of high light.
18) Manufacturers are responsible for providing electromagnetic compatibility information of
equipment to customers or users.
19) The user has the responsibility to ensure the electromagnetic compatibility of the equipment, so
that the equipment can work properly.
20) In dry environments, especially in the drying environment of artificial materials (artificial fabrics,
carpets, etc.), the use of this equipment may cause damage to electrostatic discharges, resulting in
erroneous conclusions.
21) Prohibit the use of this device near a strong radiation source (such as a non shielded radio
frequency source), or it may interfere with the normal operation of the equipment.
22) This equipment is designed and tested according to class a equipment in GB4824. In the home
environment, this device may cause radio interference and requires protective measures.
23) It is recommended that the electromagnetic environment be evaluated prior to the use of the
equipment.
24) This equipment complies with the emission and immunity requirements specified by
GB/T18268.26.
Note: The instrument used reagents and samples may be corrosive or infectious, please pay
1
attention to the protection of instrument when do the operation, can use protective gloves, do not
come into contact with the skin, the waste must be in strict accordance with the management of
medical waste treatment system.
Note: The Microplate Reader is very professional precision instrument, the equipment
maintenance must be conducted by professional staff .When maintenance, repair, or movement
should do the cleaning and disinfection of instruments so as to minimize biological hazards, the
company promises to respond to user needs at any time, don't agree with non-professional
personnel engaged in the work related to the maintenance, repair, our company is not responsible
for the consequences.
1.2 Symbol
The following table lists the symbols used on the Microplate Reader and their significance.
Protective conductor
The symbol is marked to indicate ground protection.
terminal
2
2.2 Main Performance
Wavelength accuracy and transmission character of the filters should meet the following
requirements:
Item Indicator
Indication error of wavelength(nm) ±1.0
Half width(nm) ≤8
Peak transmittance(%) ≥35
-Measured value stability:±0.003A
-Absorbance indication error (Accuracy):±0.008A
-Absorbance repeatability: ≤0.2%
-Sensibility:≥0.01(L/mg)
-Channel error: ≤0.01A
2.3 Main Function
-Large screen interface, touch screen and touch pen input mode make it convenient for users to input
information.
-More than 500 programmable laboratory tests.
-Four measurement method: Single wavelength, Double wavelength, two point method, kinetic
-Several quantitative and qualitative methods: ABS, Cut-Off regression,Single-point calibration,
broken line regression, linear regression, exponential regression, logarithm regression, double
logarithm regression, log-logit, power regression.
-96-well visual plate. Blank hole, contrast hole, sample hole, standard hole and quality control hole
are adjustable.
-Conduct 12 different tests in one plate at the same time.
-Fast and accurate testing with 8 channels (≤10S/ Plate ) .
-Flexible synthesis report output, supporting internal thermal printer and printer with USB interface
2.4 Main Structure and Components
The instrument is made up of switch power supply, optical path system, inner computer system
and sample transmission system.
Front view: ③
②
①
① Power indicator light : After the instrument is power on, the indicator light is lit.
② Touch screen: Show software interface. Users can press the screen with fingers for variable
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operation
③ Internal thermal printer: Plastic cover plate. Paper delivery when using internal thermal printer.
You can open the cover plate to replace paper.
④ ELISA plate cover plate: Plastic cover plate. Prevent dust from polluting inner space of ELISA
plate and instrument.
Back view:
⑤ ⑥ ⑦ ⑧ ⑨
④ ④
① ② ③ ⑩
protective grounding of the machine unified using with logo .The AC power supply must be
stable, don’t share power with high-power electrical appliances. When unplug the power line,, must
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grasp the plug itself, rather than the power line. If discover there is smoke, strange smell or strange
sound, immediately shut off the power and contact the seller.
3.6 Start installing
--Connect instrument to power source;
--Put one port of power line in power socket of the instrument ;
--Put the other port of power line in AC power socket.
3.7 Print paper installation
3.7.1 Press the printer cover plate, then remove the printer cover (figure 1).
3.7.2 Pull out of the printer roller and place the printer paper thermal surface down to the head
direction of printer . Press the printer roller (figure 2-3).
3.7.3 Put the printer paper out of printer cover plate,cover the plate (figure 4).
Figure 1 Figure 2
Figure 3 Figure 4
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- Serum sample collection: Draw a certain amount of venous blood with single-use syringe or
vacuum blood tube, and place it at room temperature for 1-2 hours. Centrifuge with speed after
3000r/minmore than 6 min after tarombokinesis and blood clot retraction, and suck serum in
reserve. You should take care when collecting samples. It is proposed to use evacuated blood
tubes or butterfly needles tocollect samples, avoiding direct contact with blood.
Sample storage:
- Serum or plasma sample which are used for antibody detection should be stored in temperature
below -20℃. Samples which will be tested during 1 week can be stored in temperature of 2~
8℃. Plasma and blood cell samples for antigen and nuclein detection should be cryopreserved in
temperature below -20℃.
Application of sample and incubation:
- Operators should use graduated transferpettors.
- When incubating, operators should check temperature and make accurate timing before put plate
in incubator or water-bath.
Washing plates:
- It is best to use microplate washer to wash elisa plates, also set soak time and washing times
according to reagent instruction.
- When hand washing, try to avoid cross contamination.
Color development:
- Color developing reagent should be taken out from refrigerator 10 minutes before using. When
adding color developing reagent, keep dropping bottle straight down and holding pressure
uniform, besides dripping speed not too fast. Do not mix reagent A and B before adding, you
should add A and B in turn.
Reading plates:
- After color development, operators should use microplate reader to read the elisa plates during
required time of the instructions’.
- After reading plates, operators should process sample plates as pollutant.
Waste processing
- Regard kit as infectious substance and process it according to laboratory regulation about
infectious disease. ( Refer to reagent instruction’s requirements).
Warning:
The Microplate Reader use touch screen: do not use sharp or hardness of objects (such as
metal, glass, nails, etc.) touch screen surface; Part of touch screen is glass, do not exert great
impact on touch screen, so as to avoid damage.
The grounding of AC power supply must be good, otherwise it may affect the sensitivity of touch
screen.
Note: The Microplate Reader can be equipped with an external mouse, keyboard.
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4.2 Digital soft keyboard
The enter window can be used to input integers (such as sample numbers, etc.), decimals (such
as concentration values etc.). The enter content consists of 0~9 and decimal points. After completing
enter, press the “ OK ” button to confirm ,or press “ Cancel ” button to abandon content.
Change the content: Press the "Back" button to delete the first character of entered ; Press the
"Clear" button to clear all the content you've entered.
The window title bar is prompted with enter content and its limits. If user enter exceeds the limit,
the system will be discarded automatically. The system has the following restrictions on user enter
content:
1) The value entered shall not exceed the max or min; If the min is non-negative, press the "-"
button is invalid.
2)When enter an integer, press "." button is invalid.
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4.4 Date、time soft keyboard
For entering date and time, sliding the number up or down can change the current selection state.
Section 5 Start-up
5.1 Procedure of start-up
Turn on the power switch at the rear panel. Wait about 6 seconds, the instrument will perform an
initialization process.
The procedure of start-up includes:
1)Load the program;
2)Access the user data;
3)light road and mechanical self-examination: during the period, the filter wheel will rotate, and the
enzyme plate will approach a round trip.
If an error occurs during initialization, the system pops up a window to report an error message. Users can
refer to the "Simple fault handling" section of this manual “Instrument maintenance”. If can’t solve it,
please contact the seller.
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Section 6 System
Click “System” button in the main menu and go into the system setting menu.
6.1 Main Menu
Select the “System” button and go into the system setting menu:
1)Serial No.(ID): This number is read-only and cannot be modified;
2)Company Name: No more than 20 characters (including Chinese and English characters);
3)Report Title: No more than 20 characters (including Chinese and English characters;
4) LCD& Sound: Click "<<" or ">>" to adjust the contrast of the LCD screen;
5) Sound : Click to activate or close the beep sound;
6) Regional: Modify the time display format and system language;
7) Date&Time :The current date and time can be entered;
8) Print:Select the printer model, which supports KX-P1121 needle printer/EPSON-LQ300K+ needle
printer/ Built-in thermal printer;
9)Work Mode : Select Stand-Along or PC mode work;
10) Input Device : Select the current mode of operation as the Key & Mouse or Touch input;
11) Maintenance: Provides a range of system detection functions, including Add Filters, Lamp Test, Repeat
Test and so on;After you modify the parameter, click “OK “ button to save the settings, and then click
“Back” button to quit.
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Click the ‘Maintenance’ button to go into the system maintenance menu.
Add Filters: First select the number of filters, then fill each filter wavelength corresponding each
well, click "OK" button.
Lamp Test: The function is mainly used to replace the lamp. After selected the filter, click the
"Start" button, and then manually adjust the position of the lamp, while observing the test value;
adjust to the maximum value, fixed the lamp.
Repeat Test: This function is mainly used for detecting instrument performance. Select filter, test
times and row to test, the system will calculate the average value, CV value, and repeatability
value automatically.
Self Test:This function mainly test if the mechanical parts of the instrument can be used
normally.
Background Color Set:This function sets the background color.
Advanced: This module is only used by the serviceman/manufacture.
6.3 Dictionary
In the main menu, click the ‘Dictionary’ to go into the editing interface of dictionary.
This window provides addition, modification and deletion for seven dictionary information.Including
Clinical Lab, Submit Dep., Lab Doc.,Submit Doc. ,Sample,Diagnosis,Unit.
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Add: Click the “Add ” button and then enter the relevant information in the soft keyboard.
Modify: First select a dictionary item then click the “Modify ” and enter the relevant information in
the soft keyboard.
Delete: First select a dictionary item then click the “Delete”,delete the selected dictionary item.
The Microplate Reader all items are defined by the user. Items that have been set by the user are
displayed in the window list.
7.1 Measure method
The reader has four measure methods.
7.1.1 Single wavelength
The method uses one wavelength to measure the OD.
7.1.2 Dual wavelength
The method uses two wavelengths to measure the OD. The result of OD is the difference of the main
wavelength and the second wavelength.
7.1.3 Fixed time
Absorbance values were measured at two different time points. The absorbance value is the difference
between the second detection value and the first detection value.
7.1.4 Kinetics
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The absorbance was measured several times according to the interval between the intermittent time
and the delay time. According to the slope of the absorbance curve obtained by the regression, the
absorbance value of 1 minutes was used as the absorbance value, and the concentration value was
calculated.
Note: the kinetic method uses only one wavelength.
7.2 Analysis method
There are 9 analysis methods, divided into the following 3 categories:
7.2.1 O.D. mode
The absorbance of the sample is measured and output directly.
7.2.2 Qualitative(Cut-Off) mode
Cut-Off limit formula: Cov X NC Y PC Fac
NC is the O.D. of the negative reference material,PC is the O.D. of the positive reference material,
X,Y,Fac is the coefficient,which is set in the reagent instruction.All the other kind of qualitative formula
can convert into this formula format.For example:if Sample OD/Negative Ref.OD≥2.1 is the Positive,then
X=2.1,Y=0, Fac=0。
The result of qualitative calculation is expressed by the ratio of sample absorbance to cut-off
threshold, the unit is s/co. Generally, the value of negative and positive is determined with 1 as the critical
value.
7.2.3 Quantitative mode
1) Single regress:need to set 1 standard, take the line of origin and standard point as the calibration
curve. Single regress must set blank to calibrate 0.(X:concentrate,Y:O.D.):
O.D.
C
2) Multi regress(point - point) :allow set 2-8 standard samples ,take each standard point as the
calibration curve (should be a monotonically ascending or downward fold) :
O.D. O.D.
C C
If the absorbance of the standard sample does not increase monotonically or decrease monotonically,
the calibration result is error:
O.D.
C
3) Linear regress:allow set 2-8 standard samples,a straight line Y kX b is regressed by these
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standard points as a calibration curve:
O.D.
standard points as a calibration curve,all the standard sample O.D. values must be >0 .
If the absorbance value is logarithmic ( Y LnY ), the exponential regression can be transformed
into linear regression: Y k X b .
5) Log Regress:allow set 2-8 standard samples,a logarithmic curve Y kLnX b is regressed by
these standard points as a calibration curve,all the standard sample concentration values must be >0 .
If the concentration is logarithmic ( X LnX ), the logarithmic regression can also be transformed
into linear regression: Y kX b .
( kLnX b )
6) Dual Log:allow set 2-8 standard samples,a double logarithmic curve Y 10 is regressed
by these standard points as a calibration curve,all the standard sample concentration values must be >0 .
If the concentration is logarithmic ( X LnX , Y LnY ), the logarithmic regression can also be
transformed into linear regression: Y k X b
Power Regress:allow set 2-8 standard samples,a power curve Y kX is regressed by these
b
7)
standard points as a calibration curve,all the standard sample O.D. values and concentration values must
be >0 .
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Blank : The blank contrast absorbance value of the actual test.
● Ref. Area: The scope of the calculation results of the item.Some items can be entered 0.000 ~ 0.000 if
the user does not need to print the reference range.
● Sort No.: Define the sort of the item in the patient report. The small No. is in the front of the list. The
default value is 100.
● ABS analysis methods:Press the “OK ”to save the item information and press the “Cancel” to exit the
settings for the current items;
In addition to ABS, other calculations can be found on “Standard/ Conrtol Settings”, “Qualitative
Setting” and “Quality Control Setting".
If the analysis method is Cut-Off, click “Standard/ Conrtol Settings” to view the menu:
● Negative/Positive Ref Area:The valid reference area of the Negative and Positive,the default value is
0.000~4.000;
● Negative/Positive 1~4:User can see the OD of the Negative/Positive after test, or set the OD directly
without test.Then click “Qualitative Setting” .
● Qualitative Group Name:User can define the name or signal of every group;
Click the label “Group 1”~“Group 5” to set the predefined name,which will shift between the
“Negative,Weak negative,Grey Area,Weak positive,Positive”;
● Formula(Cut-Off value):User can define the C.O. formula or value of every group, four formulas at
most;
The relation of the Cut-Off value、OD value and qualitative is:
- +
Cut-Off value OD
From the manual of the ELISA kit, the formula of the Cut Off is “Cut Off value=Negative × 2.1”, so
the formula should be 2.1*NC1;If there are comments like“Minimum Negative OD = 0.05 ”,the meaning
of the comments is to get the large value of the NC1 and 0.05,so the final C.O. formula is:
2.1*MAX(NC1,0.05)
This is the formula of the Group 1. There are four formulas at all. And the value of the formula must
be “Formula 4 >Formula 3> Formula 2> Formula 1”. The five groups have four formulas.
If the item has more formulas, the CO value in S/CO could be selected from the four formulas. The
default value is 1.
If the formula is longer than the viewing area, just click the formula before the group label to show
the full formula in the last line.
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In addition to the formulas Cut-Off and ABS, click the “Standard/ Conrtol Settings” to display the
quantitative settings interface, and you can modify the following parameters:
● STD numbers: In addition to Single regress only can set 1 standard samples ,others can set 2~8
standard samples.
● Dilute Multiple:Define the dilute multiple of the sample, and the calculated value should multiply the
dilute multiple.
● Unit: Choose unit from the 24 common units in the list.
● Dual STD: If check the dual STD,all the standards in the plate will be positioned double times.
Di
● ABS percent: Check the box to use 100% as the ordinate of the standard graph,the D0 is the
D0
reference standard of 100% O.D., and the D0 needs no concentrate. If the item selects the ABS percent, the
first standard is D0 when position the plate. The dual logarithm regression cannot use the ABS percent
because of the ordinate is in logarithm.
● STD concentrate table: Display and select the STD concentrate. Attention: All the STD concentrate
should increase.
● Modify concentration: Modify the selected concentration.
Then click “ Qualitative Setting” .
● Qualitative Group Name:User can define the name or signal of every group;
Click the label “Group 1”~“Group 5” to set the predefined name,which will shift between the
“Negative,Weak negative,Grey Area,Weak positive,Positive”;
● Formula(Cut-Off value):Users can define values or formulas for each grouping, while supporting
multiple formulas.
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If the item needs to set quality control, you can click “Quality Control Setting”. This instrument
supports the known X, SD method, L-J method and instant quality control method.
Section 8 Test
8.1 Test setting
Click the test button on the main screen and enter the test parameter settings window.
8.3 Templet
Click the “Import Template” button to display the template selection window.
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Select an existing template and click “OK “to import the template.
Contains all of the information in the template layout: layout direction, moving mode, item no ,type.
The The Microplate Reader allows users to set wells at any position within the 96 orifice plate.
-The procedure of the position:
a) Click “Set” to select the item;
b) Click “S,B,N,P,D,Q,C” to select the type of the sample;
If you need multi item board layout., click on the "2nd" or ">>" button, select the item and layout
again. Repeat the steps until the completion of the board layout.
Well mark symbol:
● Sample:S
● Blank:B
● Negative contrast:N
● Positive contrast:P
● Standard:D
● Quality Control :Q
● Clear:C
-Sample
User could click any hole to set the Sample.Click the hole again to modify the Sample No.The area of
the Sample No. is from 1 to 999.
-Blank
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The blank is used to do zero adjustment.It means that all the other hole’s OD should minus the Blank
OD.If the Blank is not positioned, the default blank is zero.
Every item can position more than one blank wells.If there are several blank holes in the item, the
result only displays one mean blank value(average value).
-Negative contrast
The negative contrast is valid only in the Cut-Off items.User must position the negative contrast
according to the Cut-Off formula.Every item can position one or more negative contrast. If there are
multiple contrast values in one item, the result displays each contrast value.
The absorbance value of the contrast well in the test of the item will be automatically saved. In the
follow-up test of the same item, if the contrast is not reset, the system will use the old contrast value for
calibration.
-Positive contrast
Positive contrast is set as same as Negative contrast.
-Standard
Valid when standard calibration is required in item setting. If the item already has old standards, users
can choose not to set standards (use the old standard calibration) or reset all the standard samples, not
allow only set part of standard samples. Users click on the standard well, and the system also marks it D1,
D2.. If the standard has not been set, the user begin doing the test, the system will prompt "Standard layout
error".
Note: If a standard is set up in a item test and the calibration result is correct, the new standard will
cover the old standard of the item .
-Clear
User can click the hole to clear after select the “Clear” button. And only the hole of the current item
can be cleared.
-Batch
Press "Set" , then press “Batch ”, only the sample can be batch positioned. In the batch sheet window,
set the starting position, the end position and the initial sample number, and press "OK".
-Templet
Press "Set" ,then press “Board Templet ” to enter the template setting window. The template is a
specific type of cloth board, which can be set according to the usage habit, simplifying the daily fabric
operation. In the template window, you can add, delete, and cover templates.
-Choose way
There are four ways of orifice plate, after selected well position type:
● Click the well to set a well;
● Click the number(1—12)to set a column of wells;
● Click the alphabet(A—H)to set a line of wells;
● Click the “*” to set all the wells.
All the ways is valid to “Clear ”operation.With the function of "*", the cloth board of large quantity
samples can be carried out quickly.
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8.6 Result view and print
After the plate reading, automatically enter the test result window.
.
If the layout for the multi item layout, convenient for users to view data from the display, you can
select the item to display from the display item drop-down list, unrelated items will be shielding.
Click "Layout" to check the condition of layout board.
Click "ABS" to view the absorbance value and click the single sample well position to modify the
sample absorbance value.
Note: If the measured absorbance is > 4.000 A, it is shown as 4.00 *; < 0.000A will show as 0.00 *.
Click “Qualitative” to view the qualitative of the result according to the setting of the item.
Click “Quantify” to view the calculated value. In the Cut-Off item, the quantitative value is the value
of S/CO. But in the quantitative item, the value is the calculated concentration.
Click “Save” to save all the values of the plate. The memory of the instrument can save more than two
thousand plates.Save success will prompt "Save success!"
Click “Print” to print all the values by the position. The holes that not positioned will be neglected
automaticly.
Click “ Back ” to layout interface.
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9.1 Basic operation
Includes adding, modifying, and deleting patient information.
Operation procedure:
First of all, enter the test date, the form will list the sample no. and patient name for the specified date
be based on patient information and samples of the test.
Click the “Add” button to add a new patient message;
Select a patient item and click the “Modify” button to modify a new patient message;
Select a patient item and click the “Delete” button to delete patient information from the selected
patient.
Click the “ Last Page” & “ Next Page ” to display the patient information on the last page or next
page.
Note: If you add patient information, be sure to check the date of submit date and test date, otherwise there
may be errors.
Section 10 Search
Click the ‘Search’ icon in the main menu to go into the menu of data searching.
You can enter the send date , test date , name, sample number to search,search conditions is one of
them.
For example: the send date is "2017-04-01", and all patients on 2017-04-01 can be searched.
For example, the sample number is 12, and all patients with a sample number of 12 can be searched.
● Select a patient item and click the “Sample report” button to enter the sample report window. View
patient details at this window. Such as item name, result, unit / figurate and normal range of the item. Can
also print, delete the sample report.
● Select a patient item and click “Patient information” button to enter the patient information window
to view the patient's information.
● After select a patient item,the first column of the table will appear the words "P", click again can
cancel it .Can click “ Select all ” , add "P" to multiple patient items, click “Print” can continuously print
multiple patient report.
Click “Last page ” & “ Next page ” to view other patient information.
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10.2 Item Report
Press the "Item report" button and click "ok" to enter the itemst test result search. Search according to
Item name and Test date .
The item report window displays the patient's name, sample number, result, and figurate of all test
results which is specified item and date. You can also click "Print" to print the item.
The contents of the board report are in accordance with the test results.
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The Cut-Off item shows absorbance and formula values for each contrast sample.
The quantitative items show the concentration and absorbance of each standard sample and show
curves simultaneously.
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● Quality control data: Quality control data found.
● Quality control picture: A quality control chart drawn from quality control data.
Section 11 Report
In the main interface, press the "Report" button to enter the comprehensive report window. This
button is ashortcut to check patient reports for the day.
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Section 12 PC Control
12.1 Software installation
The instrument can be controlled by the PC machine through the serial port, using PC interface
software, more powerful, more convenient to connect to hospital management system, if using this method,
the instrument must be set as follows: (the software instructions, please refer to the online help software)
● Click on "System "
● According to the connection mode with PC, in the working mode select "PC mode RS 232" or
"PC mode USB""
● Click "Save"
Note:If you need to install the software, please contact the agent or manufacturer. After setting to PC
mode, the “Test” button on the main interface will be clicked invalid.
Note: Keep instrument away from dissolvent, oil and corrosive substance.
Note: The lamp must be purchased with the specified brand and model.
Motherboard failure,
contact distributor or
manufacturers to change
the motherboard
External printer connect System setup>Set
9 External printer error
later Printer
Open the machine cover,
check if the drive motor is
rotating
Drive motor fault
10 Plate holder don’t move On the data board,
Plate holder reset error
Holzer components can not
connect the magnetic steel of
the plate holder
External printer unable Printer power is not normal Check if power plug is loose
11
to start Check ON/OFF button
Check if printer type is
setting correct
External printer unable
12 Printer setting is not correct Check if print cable is
to print
normal connection
If printer is setting normal
External printer appears Replace the cartridge or
13 to fade, print quality Printer problem ribbon, clean the print head
decline (see the printer user's manual)
External printer paper (See the printer user's
14 Printer problem
jam and other faults manual)
Check whether plate
holder transverse plate is
falling off, shifting
Plate holder positioning Check whether 12
Plate holder transverse
15 detection signal positioning grooves on the
position is not normal
abnormal transverse plate is blocked
Check plate holder
positioning detection slot
coupler
Xxx wavelength air gap Bulb broken Replace bulb
16
is too low Filter broken Replace filter
Check filter and filter wheel
Xxx wavelength air gap Filter not properly installed
17 are properly installed
is too high Filter broken
Replace corresponding filter
Front end data The serial line between Reconnection 3 core serial
18
acquisition board is not front board and data line
31
responding acquisition board is loose Reinstall the software's
USB communication built-in USB drive
cannot identify USB
signals
Not detected the plate
holder reset signal(Not
Check the plate holder motion
necessarily signal error, it is
stroke length and force
Plate moving test possible that the distance is
19 condition
error too long) (The error is
Check the position of slot
related to the position of
coupler
the reset signal and slot
coupler )
In the Cut - Off
formula,calculated CO
value is 0;
Or in the quantitative
formula, a standard ABS
value exceeds the range of
Plate moving test data Check project setup and elisa
20 0.000-4.000;
error plate layout situation
Or in the quantitative
formula, unable to get the
curve after calculation, for
example two different
concentrations of standard
ABS values are the same.
Read the reagent user manual
The concentration values of
carefully and understand the
Standard concentration multiple standard must be
21 interpretation of the
must be increasing increasing in the project
specification for standard
setup
solubility
The quantity of standard
wells does not match with
Carefully verify the number
the quantity of standard
of standard items in the
22 Standard layout error which is setted in the
reagent user manual
project in the number of
items set in the standard
quantity
New projects do the plate
moving for the first
time,projects require setting It is recommended that
contrast but layout do not the client set a negative and
Contrast plate
23 not set (If the project positive contrast well every
arrangement error
require there are negative time when they do the
contrast and positive experiment
contrast at the same time ,
layout must do both)
Confirm that there is no
Negative control blank participation
24 Negative control value<0
absorbance too low calculation under the double
wavelength
Check if foreign objects are
Negative control Negative control value > stained with lenses, if the
25
absorbance too high 4.0 experiment fails,the solubility
of the sample is too deep
Confirm that under the dual
Positive control wavelength there is not blank
26 Positive control value<0
absorbance too low to participate in the
calculation
Check if foreign objects are
Positive control stained with lenses, if the
27 Positive control value>4.0
absorbance too high experiment fails,the solubility
of the sample is too deep
28 Cut-Off value error Cut-Off value ≤0 Check if the qualitative
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formula is wrong
Confirm that there is no blank
Standard absorbance
29 Standard absorbance ≤0 participation calculation
too low
under the double wavelength
Check if foreign objects are
Standard absorbance stained with lenses, if the
30 Standard absorbance ≥4.0
too high experiment fails,the solubility
of the sample is too deep
Standard absorbance Whether the test failed,
incorrect such as same whether the washing plate
31 Standard value error absorbance value of and adding sample is correct
standard with different and whether there is cross
concentration contamination
Note: In the use of the process if users can not solve the error, or a mistake is repeated,please contact the
seller.
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