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    Lab Exercise3 Gram Staining Lykajanetarnate PDF

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    calliemozart
    This document summarizes the results of an exercise identifying bacteria using Gram staining. Gram-positive bacteria were identified as cocci that were blue and bacilli that were purple. Gram-negative bacteria were identified as bacilli that were pink. The document also describes the function of reagents used in Gram staining and differences between Gram-positive and Gram-negative cell walls such as thickness of peptidoglycan layer and presence of lipopolysaccharides.

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    Lab Exercise3 Gram Staining Lykajanetarnate PDF

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    calliemozart
    76 views3 pages
    This document summarizes the results of an exercise identifying bacteria using Gram staining. Gram-positive bacteria were identified as cocci that were blue and bacilli that were purple. Gram-negative bacteria were identified as bacilli that were pink. The document also describes the function of reagents used in Gram staining and differences between Gram-positive and Gram-negative cell walls such as thickness of peptidoglycan layer and presence of lipopolysaccharides.

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    LAB_EXERCISE3_GRAM_STAINING_LYKAJANETARNATE.pdf

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    This document summarizes the results of an exercise identifying bacteria using Gram staining. Gram-positive bacteria were identified as cocci that were blue and bacilli that were purple. Gram-negative bacteria were identified as bacilli that were pink. The document also describes the function of reagents used in Gram staining and differences between Gram-positive and Gram-negative cell walls such as thickness of peptidoglycan layer and presence of lipopolysaccharides.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    76 views3 pages

    Lab Exercise3 Gram Staining Lykajanetarnate PDF

    Uploaded by

    calliemozart
    This document summarizes the results of an exercise identifying bacteria using Gram staining. Gram-positive bacteria were identified as cocci that were blue and bacilli that were purple. Gram-negative bacteria were identified as bacilli that were pink. The document also describes the function of reagents used in Gram staining and differences between Gram-positive and Gram-negative cell walls such as thickness of peptidoglycan layer and presence of lipopolysaccharides.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    Catanduanes State University

    Virac, Catanduanes

    LYKA JANE T. TARNATE


    BSN-1D

    EXERCISE NO. 3 GRAM-STAINING

    I. Give the Gram-staining reaction and morphology (shape) of the following bacteria.

    Gram stain reaction: Blue (Gram-positive)


    Morphology/Shape: Cocci

    Gram stain reaction: Purple (Gram-positive)


    Morphology/Shape: Bacilli

    Gram stain reaction: Pink (Gram-negative)


    Morphology/Shape: Bacilli

    This study source was downloaded by 100000880323897 from CourseHero.com on 02-05-2024 09:03:23 GMT -06:00

    https://www.coursehero.com/file/133274405/LAB-EXERCISE3-GRAM-STAINING-LYKAJANETARNATEpdf/
    II. Indicate the function of the different reagents used in Gram-staining

    Expected Result
    Reagent Function
    Gram-positive Gram-Negative

    Crystal violet Primary stain Purple or blue Purple or blue

    Gram’s iodine Mordant Purple or blue Purple or Blue

    95% Alcohol Decolorizer Purple or blue Colorless

    Counterstain or
    Safranin Secondary stain Purple or blue Red or pink

    III. Differentiate gram positive cell wall from negative cell wall

    Features Gram-positive Cell Wall Gram-negative Cell Wall

    Peptidoglycan
    Have a thick peptidoglycan Have a thin peptidoglycan
    layer and no other lipid layer and have an outer lipid
    membrane whist membrane

    Complexity
    Simple Complex

    Teichoic acid Absent


    These are essential to
    viability in the environment
    and provide chemical and
    physical protection

    Lipopolysaccharides complexes Absent -Present


    -any of a large class of lipids
    conjugated with
    polysaccharides

    This study source was downloaded by 100000880323897 from CourseHero.com on 02-05-2024 09:03:23 GMT -06:00

    https://www.coursehero.com/file/133274405/LAB-EXERCISE3-GRAM-STAINING-LYKAJANETARNATEpdf/
    Endotoxin None
    Any toxin secreted by a
    microorganism and released
    into the surrounding
    environment only when it dies

    This study source was downloaded by 100000880323897 from CourseHero.com on 02-05-2024 09:03:23 GMT -06:00

    https://www.coursehero.com/file/133274405/LAB-EXERCISE3-GRAM-STAINING-LYKAJANETARNATEpdf/
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