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23AS102 - Chemistry Lab - Short Procedure

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29 views7 pages

23AS102 - Chemistry Lab - Short Procedure

Uploaded by

Sukhee Sakthivel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DEPARTMENT OF SCIENCE AND HUMANITIES

Subject: APPLIED SCIENCE - CHEMISTRY LABORATORY

Subject Code: 23AS102

SHORT PROCEDURE
DETERMINATION OF DISSOLVED OXYGEN IN WASTE WATER USING
WINKLER’S TITRIMETRY METHOD

SHORT PROCEDURE
AIM

To determine the amount of dissolved oxygen present in the given water sample by
Winkler’s method.

CHEMICALS REQUIRED
Potassium dichromate, Sodium thio-sulphate, Potassium iodide, Starch, Sulphuric
acid, Manganous sulphate.

TITRATION1: I STANDARDISATION OF SODIUM THIOSULPHATE

Potassium Dichromate Vs. Sodium Thiosulphate

Burette solution : Sodium thiosulphate


Pipette solution : Potassium dichromate (K2Cr2O7)
Reagents added : 10 ml of KI+5ml of dil H2SO4
Indicator : Starch
End Point : Colour change from blue to green

TITRATION – II: ESTIMATION OF DISSOLVED OXYGEN

Water Sample Vs Sodium Thiosulphate

Burette solution : Sodium thiosulphate


Pipette solution : water sample
Reagents added : 2 ml Mn(SO4)2 + 2 ml alkali iodide
Indicator : Starch
End Point : Disappearance of blue colour
DETERMINATION OF STRENGTH OF STRONG ACID BY pH METRY

SHORT PROCEDURE
AIM
To determine the strength of given hydrochloric acid by pH metry. You are provided with a
standard solution of NaOH of strength 0.2 N.

MATERIALS REQUIRED
pH Meter, Combined glass electrode, 100 ml beaker, Burette, Pipette, glass rod, HCl, NaOH
solution.

Burette solution : Sodium Hydroxide (NaOH)


Pipette solution : Dil. HCl
Reagents added : 20 ml of distilled water
Electrodes : Combined glass electrode

Model Graph

Model Graph: Volume of NaOH Vs pH

Model Graph: Volume of NaOH Vs ΔpH/ΔV


DETERMINATION OF SINGLE ELECTRODE POTENTIAL OF AN
ELECTRODE

SHORT PROCEDURE

AIM
To determine the single electrode potential of zinc and copper in different concentration
solution by potentiometric method.

MATERIALS REQUIRED
Potentiometer, Calomel electrode, Zinc electrode, Copper electrode, Graduated pipette, glass
rod, 100 ml volumetric standard flask.

Calculations

1. Copper -Calomel cell

Anode - Cu electrode

Cathode - Saturated calomel electrode

Electrolyte - CuSO4

Ecalomel = 0.2422 V

ECu = Ecell + Ecalomel

2. Zinc-Calomel cell

Anode - Saturated calomel electrode

Cathode - Zn electrode

Electrolyte - ZnSO4

Ecalomel = 0.2422 V

Ezn = Ecalomel - Ecell


ELECTROPLATING OF NICKEL AND DETERMINATION OF CATHODE EFFICIENCY

SHORT PROCEDURE

AIM:

To determine the cathode efficiency of nickel plating.

APPARATUS REQUIRED

DC power supply, beaker, copper plate, nickel plate.

Anode - Nickel Plate

Cathode - Copper Plate

Electrolyte - 20 ml of NiSO4 + 20 ml of Ni Chloride + 10 ml of Boric acid

Current density - 2A, 4A & 6A


ESTIMATION OF IRON IN WATER BY SPECTROPHOTOMETRY

SHORT PROCEDURE

AIM:

To estimate the amount of iron present in a given samples of water using spectrophotometer.

APPARATUS REQUIRED

Spectrophotometer, Ferrous ammonium sulfate solution, KmnO4, H2SO4, HNO3, KSCN, Volumetric
standard flask 100 ml, Cuvettes, Graduate pipette.

PRINCIPLE
I0
log = ct
It

I0
log =A
It
Where, A = ct
I0 = Intensity of incident light
It = Intensity of transmitted light
C = Concentration of solution
t = Thickness of the cell
 = Molar absorption co – efficient

OBSERVATION:
λmax = 480 nm

1 ml = 1 mg/l of iron (Ferric ion, Fe3+)

Preparation of various concentration of Iron solution

VOLUME VOLUME VOLUME VOLUME FINAL CONCENTRATION


OF IRON OF 10% OF 10% OF WATER VOLUME OF IRON
S.No.
SOLUTION HNO3 (ml) KCNS (ml) (ml) (ml) SOLUTION (mg/l)
(ml)
1. - 3 5 92 100 Blank Solution
2. 1 3 5 91 100 1
3. 3 3 5 89 100 3
4. 5 3 5 87 100 5
5. 7 3 5 85 100 7
6. 9 3 5 83 100 9
7. Unknown 3 5 -- 100 --
Model Graph: Concentration of FAS Vs Absorbance (OD)

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