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L1F21MSMR004

The document provides details about a research synopsis submitted by a student named Samman for their MS research. The research aims to characterize multi-drug resistant Klebsiella pneumoniae strains found in hospitalized patients. The student plans to collect clinical samples, isolate K. pneumoniae, test antibiotic susceptibility, and use PCR to detect drug resistance genes. The research aims to determine the prevalence of K. pneumoniae, antibiotic resistance patterns, and identify resistance genes, which could help address the growing problem of antibiotic resistance posed by this pathogen.

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Samman zaka
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16 views4 pages

L1F21MSMR004

The document provides details about a research synopsis submitted by a student named Samman for their MS research. The research aims to characterize multi-drug resistant Klebsiella pneumoniae strains found in hospitalized patients. The student plans to collect clinical samples, isolate K. pneumoniae, test antibiotic susceptibility, and use PCR to detect drug resistance genes. The research aims to determine the prevalence of K. pneumoniae, antibiotic resistance patterns, and identify resistance genes, which could help address the growing problem of antibiotic resistance posed by this pathogen.

Uploaded by

Samman zaka
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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MOLECULAR CHARACTERIZATION OF MULTI DRUG RESISTANT

KLEBSIELLA PNEUMONIAE IN HOSPITALIZED PATIENTS

University of Central Punjab Faculty


of Science and Technology
Department of Microbiology
Research Synopsis for MS / MPhil

Name of Student: SAMMAN


Registration/Roll N o . L 1 F 2 1 M S M R 0 0 0 4
Date of Registration:
Class (MS / MPhil): MS RESEARCH
Session: 2021-2023
Couse Code: MB6916
Supervisor: D R S A B A K A B I R
Co-Supervisor ( if a n y ): DR FARHAN RASHEED

Title of Research:

MOLECULAR CHARACTERIZATION OF MULTI DRUG RESISTANT KLEBSIELLA


PNEUMONIAE IN HOSPITALIZED PATIENTS
Introduction :

Klebsiella pneumoniae, belonging to the Enterobacteriaceae family, is a natural inhabitant of the


gastrointestinal tract microbiome of healthy human and animals. It is a common opportunistic
hospital-associated pathogen, accounting for about one third of all Gram-negative infections
overall. It is involved in extra-intestinal infections including urinary tract infections, cystitis,
pneumoniae, surgical wound infections and life-threatening infections, such as endocarditis and
septicemia. (Haryani, Noorzaleha et al. 2007)
Due to the high rates of resistance to antibiotics, K. pneumoniae was considered as one of the most
frequent agents of infectious diseases and significant menace to public health . The resistance to
antibiotics is a complex multifactorial process. Nevertheless, it reflects evolution in action, related
to the continuous exposure to antibiotics.(Navon-Venezia, Kondratyeva et al. 2017) Consequently,
the selective pressure gives rise to the development of many genetic mechanisms . The acquired
resistance over the years has led to the emergence of multidrug-resistant (MDR) and extensively
drug-resistant (XDR) strains that exhibit resistance to nearly all available antibiotics, without
possible treatment options.(Falagas and Karageorgopoulos 2008) The resistance of K. pneumoniae
to many antibiotics is due to multiple mechanisms such as modification of antibiotic target sites,
alteration of metabolic pathways, activation of efflux pump systems, change in the permeability of
membrane and release of antibiotic-inactivating enzymes.(Lambert 2005)
Klebsiella pneumoniae has been associated with different types of infections and one of the most
important aspects of Klebsiella is the emergence of multi-drug resistant strains particularly those
involved in nosocomial diseases. Klebsiella pneumoniae plays a major role in the worldwide
burden of antibiotic resistance. (Blot, Depuydt et al. 2007)
Problem statement:
Klebsiella pneumoniae plays a major role in the worldwide burden of antibiotic resistance. In this era of
antibiotic resistance, Klebsiella pneumoniae represents one of the most concerning pathogens involved in
antibiotic resistance and as such, together with other highly important MDR pathogens. Recently, World
Health Organization also warned the community that multidrug resistant bacteria are emerging worldwide
which is a big challenge to healthcare.

Objectives of Study:

The key objectives of the study are:


 To check the prevalence Klebsiella pneumoniae among hospitalized patients
 To check the antibiotics suspectibility pattern of Klebsiella pneumonia strains against high
generation of antibiotics
 Molecular detection of Klebsiella pneumonia of drug resistance genes
Hypothesis:

Plan of Work:
Sample
Total of 100 clinical samples of pus, swab, urine, Blood, catheters, bronchial washings and CSF
etc will be collected from hospital and utilized.
Sample processing
Isolation of K. pneumonia isolates will be culture.
Biochemical Test
Antibiotics sensitivity test
Antibiotic sensitivity of clinical K. pneumoniae isolates will be done by Bauer’s and Kirby’s disc
diffusion method. Some of the antibiotics that will be used is mentioned in Table 1.
Table 1: Antibiotics to be used for sensitivity testing
Aminoglycosi Carbapen Cephalosporin Fluoroquinolones Monobactam Tetracycline Macrolide
des mes s

Gentamycin Imipenem Cephradine Norfloxiacin Aztreonam Doxycycline Erythromycin


Tobramycin Meropene Cephalexin Ciproflaxiacin Tetracycline Clindamycin
Amikacin m Cefadroxil Levofloxacin Clarithromyci
Kamamycin Ertapenem Cofactor Nalidixic n

Cefuroxime Ofloxacin Azthromycin

Cofactor
Cefipime
Sulbactam

DNA Extraction
DNA extraction will be carried out using Thermo scientific DNA extraction kit as per
manufacturer’s guidelines.
POLYMERASE CHAIN REACTION
The primer will be selected and design according to antibiotics sensitivity pattern of Klebsiella
pneumonia strains.

Expected Outcomes:
References:
Blot, S., et al. (2007). "Measuring the impact of multidrug resistance in nosocomial infection."
Current opinion in infectious diseases 20(4): 391-396.

Falagas, M. E. and D. E. Karageorgopoulos (2008). "Pandrug resistance (PDR), extensive drug


resistance (XDR), and multidrug resistance (MDR) among Gram-negative bacilli: need for
international harmonization in terminology." Clinical infectious diseases 46(7): 1121-1122.

Haryani, Y., et al. (2007). "Incidence of Klebsiella pneumonia in street foods sold in Malaysia and
their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis." Food
control 18(7): 847-853.

Lambert, P. A. (2005). "Bacterial resistance to antibiotics: modified target sites." Advanced drug
delivery reviews 57(10): 1471-1485.

Navon-Venezia, S., et al. (2017). "Klebsiella pneumoniae: a major worldwide source and shuttle
for antibiotic resistance." FEMS microbiology reviews 41(3): 252-275.

Signature of Student: Date:

Signature of Supervisor:

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