The Functions and Parts of A Microscope
The Functions and Parts of A Microscope
Arm: Supports the tube and connects it to the Diaphragm or Iris: Many microscopes have a
base. rotating disk under the stage. This diaphragm has
different sized holes and is used to vary the
Base: The bottom of the microscope, used for intensity and size of the cone of light that is
support. projected upward into the slide.
Eyepiece tube: It’s the eyepiece holder. It carries The Adjustment knobs – Adjustment Knobs are
the eyepiece just above the objective lens. the control knobs used to focus the microscope
on the specimen. These knobs are of two types;
Diopter Adjustment: It is a control knob present
only in the binocular microscope that is used to a. Fine Adjustment Knob: Fine Adjustment
change focus on one eyepiece. It is used to Knob is used for fine adjustment. It is a
correct any difference in vision and compensate smaller knob and is used to move the
for the differences in vision between the viewer’s stage up or down very slowly. The stage
two eyes. covers a very small distance on each
rotation of the fine adjustment knob. It
Illuminator: A steady light source (110 volts)
is used to sharpen the image. It is mostly
used in place of a mirror. If your microscope has
used while viewing under high power.
a mirror, it is used to reflect light from an external
b. Coarse Adjustment Knob: Coarse
light source up through the bottom of the stage.
Adjustment Knob is used for focusing the
Stage with Stage Clips: The flat platform where image under low power magnification. It
you place your slides. Stage clips hold the slides is a larger knob and is used to move the
in place. If your microscope has a mechanical stage up or down very rapidly. The stage
stage, you will be able to move the slide around is raised or lowered rapidly with the help
by turning two knobs. One moves it left and right, of a coarse adjustment knob.
the other moves it up and down.
Aperture: This is a hole in the microscope stage
Revolving Nosepiece or Turret: This is the part of through which the transmitted light from the
the microscope that holds two or more objective source reaches the stage.
lenses and can be rotated to easily change power.
Light Switch: Light Switch is an electrical control
Objective Lenses: Usually, you will find 3 or 4 device. Light switches are used to on and off the
objective lenses on a microscope. They almost illuminator.
always consist of 4x, 10x, 40x and 100x powers.
When coupled with a 10x (most common) Brightness Adjustment: The brightness
eyepiece lens, total magnification is 40x (4x times adjustment system controls the voltage supplied
10x), 100x, 400x and 1000x. To have good to the light bulb, controlling the intensity
resolution at 1000x, you will need a relatively (brightness) of the light bulb.
sophisticated microscope with an Abbe
condenser.