sp-50 Routine Use Training Workbook

Routine Use Training Workbook
SP-50
Date: 26/10/2020
Document Ref: SUKBMS-24-334
Version: 4.0
Classification: Unrestricted
IFU version: 1801
Sysmex House, Garamonde Drive, Wymbush, Milton Keynes, MK8 8DF, United Kingdom · Phone +44 333 320 3460 · Fax +44 1908 267 901
[email protected]· www.sysmex.co.uk
Disclaimer – SP-50
Contents
Contents 2
Disclaimer 4
Revision History 4
Reference Documents 4
SP-50 Overview 5
Analyser components 6
Outside the analyser 6
Front Components 8
Smear Unit 9
Stain Unit 10
Reagents On-board the SP-50 11
Smear Production 12
Staining Principles 13
SP-50 – IPU Layout 14
Main Screen 14
Status Area 15
Instrument Status 15
Indicator 16
Slide Preparation Mode 16
Sample Number 16
Sampler/Manual 17
Mode 17
Quick Guide: Maintenance 18
Daily Maintenance 19
Performing Shutdown 1 19
Wipe Stain Baths 21
Performing Start-up 22
Weekly Maintenance 24
Cleaning the Spreader Glass 24
Cleaning the Smear/Stain Unit 27
Cleaning the Stain Pool 30
Monthly Maintenance 31
Performing Shutdown 2 31
As Required Maintenance 34
Loading Glass Slides 34
Replace Spreader Glass 35
[email protected]· www.sysmex.co.uk 2
Replacing Ink Ribbon 39

Replacing Reagents – CellPack DCL, Stains & Buffer Solution 42
Replacing Methanol 46
Quick Guide: Running Samples 47
Sample Preparation 47
Sampler Preparation 47
Manual Preparation 47
Sample Volumes 48
Sampler Mode 49
Smearing Mode 50
Staining & Smearing Mode 50
Operation of the SP-50 on the XN Track 51
Placing into ‘Offline’ or Isolation 51
Running Samples in ‘Offline’ or Sampler Mode 51
Manual Mode 52
Manual – Smearing & Staining 52
Manual – Stain Only 56
Manual – Smear Only 59
Manual – Print Only 62
Responding to Alarms 65
Audit Logs 66
Contact Us 69
Disclaimer
Please note the information in this presentation, workbook or training session provided by Sysmex
should not be used as an alternative to your sites Standard Operating Procedure (SOP)/Contract. If you
have any particular questions regarding any site specific use of reagents, consumables and/or
equipment please contact your Management Team.
Revision History
Revised section Alteration Name Date

Daily maintenance Added wipe stain bath K. Elgerton January 2019
Weekly maintenance Amended weekly schedule K. Elgerton January 2019
Reagents Amended changing stain K. Elgerton January 2019

Audit logs Added exporting CSV K. Elgerton January 2019
Reagents Amended changing Buffer K. Elgerton January 2020
Front cover New logo added K. Elgerton January 2020
Contacts Reagent hotline updated K. Elgerton January 2020

Maintenance Shutdown times updated K. Elgerton January 2020
Title page Workbook name changed to N. Bowen October 2020
include routine use.
Reference Documents
Document title Version Date
SP-50_BO (IFU) 1801 January 2018

SP-50_GI (IFU) 1801 January 2018
SP-50_TS (IFU) 1801 January 2018
Reference Documents – SP-50 Overview
SP-50 Overview
Facts and Figures
SP-Series analysers SP-1000i
SP-10
SP-50
Throughput SP-50 S – 30 samples/hr

SP-50 H – 75 samples/hr
Capacity Standalone analyser – 50 samples

Tracked analysers – racks are automatically sent to the SP if a
smear is requested.
Sample Type Whole blood
Sampler Mode Regular tube

Raised bottom tube (RBT) – requires RBT rack
Manual Mode Manual (Closed)

Manual (Open)
Manual (RBT)
Manual (Micro)
Aspiration Volumes Sampler mode - 70µl

Manual (Closed) mode - 70 µl
Manual (Micro) mode - 38 µl
Staining Protocols May-Grunwald-Giemsa stain

Wright single stain
Wright-Giemsa stain
Analyser components – SP-50 Overview
Analyser components
Outside the analyser
1. Main Power Switch Cover – Open this cover to turn the main power switch ON/OFF, and to
regulate the pressures if required.
2. Stain Unit Cover – Open this cover to inspect the interior of the stain unit. Access can be gained
for cleaning or maintenance tasks. Locked during operation.
3. Start-up (Power) Switch – Press to start up the instrument.
4. Eject Switch – Press to eject magazines from the stain unit.
5. IPU Touchscreen – Use to operate the instrument and configure settings.
6. Sample Tube Holder – Used to set samples for manual analysis.
7. Mode Switch – Press to switch between manual analysis and sampler analysis. Pressing it
opens and closes the sample holder.
• When the sample holder is open: Manual preparation

• When the sample holder is closed: Sampler preparation
8. Start Switch – Press to start manual analysis.
9. Status Indicator LED - Shows the current status of the analyser.
LED Indicator Light Description
Green Ready (Analysis or maintenance is possible)
Flashing Green Starting up / Analysis in progress / Maintenance in progress
Orange Sample analysis stopped / not possible. An error has occurred, but the
instrument is still operational.
Flashing Orange Starting up/Preparing a slide/Switching the slide preparation mode/ Shutting
down/Sleeping
Red Stopped - Error (without alarm) / initialising system / Analyser ready to be

turned off at the end of shutdown
Flashing Red Stopped - Error(with alarm)
Not Lit Powered OFF
10. Manual Magazine Holders - This is used for loading manual slides into the analyser for manual
staining.
11. Lower Front Cover - Open this cover to inspect the interior of the main unit to perform cleaning
or maintenance tasks. Analyser must be turned off.
12. Manual Magazine Holder Status LED – Shows the current status of the manual magazine
holder. A magazine cannot be loaded or removed while the LED is flashing.
Green Containing magazine

Red Error
Not Lit Powered OFF
13. Slide Unit Cover - Open to load glass slides. Locked during operation.
14. Smear Unit Cover - Open this cover to inspect the interior of the smear unit to perform cleaning
or maintenance tasks. The smear unit cover and the slide set cover are one piece. To open the
smear unit cover, remove the slide supply cassette first. Locked during operation
Front Components
1. 0.07 MPa Regulator – Regulates the source pressure to 0.07 MPa
2. Main Power Switch – Press to turn the main power of the main unit on/off
3. Slide Supply Cassette Status LED – Shows the current status of the supply cassettes. A
cassette cannot be loaded or removed while the LED is flashing.
Green Slides loaded

Red No slides/error
Not Lit Powered OFF
4. Printer – Prints sample information and patient demographics onto glass slide.
5. Slide Supply Cassettes – Loads/stores glass slides for use on the SP-50.
Smear Unit
1. Smear Unit – Spreads the dispensed sample onto glass slide. Contains spreader glass unit.
2. Print Unit – Prints sample information and patient demographics onto glass slide. Contains the
ink ribbon.
3. Smear Dryer Unit – Dries the smeared sample.
4. Slide Supply Unit – Takes out glass slides from the supply cassettes.
Stain Unit
A
A B
A. Stain Unit Cover – Open this cover to inspect the interior of the stain unit. Access can be gained
for cleaning or maintenance tasks. Locked during operation.
B. Stain Unit – Depending upon protocol; fixes, stains, rinses and dries the prepared slides.
There are 7 staining pools which are filled up with the following reagents. The types of reagent varies
depending on the staining method. Samples move through the staining pool in the direction of the arrow
as illustrated above.
Stain Pool Role Reagent
1* Fixing Methanol
2 Fixing Stain 1
3 Staining Stain 1/buffer
4 Rinsing Rinse
5 Staining Stain 2/buffer

6 Rinsing Rinse & dry
*Use depends upon protocol
Analyser components – Reagents On-board the SP-50
Reagents On-board the SP-50

The number of on-board reagents on the SP-50 depends on the staining protocol used. The table below
summarises the reagents recommended for use on the SP-series.
Reagent Name Function On-board Stability
Cell Pack DCL Rinse solution used for rinsing the whole blood 60 days
aspiration line.
May-Grunwald stain Used for staining blood smears. N/A*
Giemsa stain Used for staining blood smears. N/A*
Buffer solution pH 7.0 Used in the preparation of blood smears. N/A*
Methanol Used in staining process depending on protocol N/A*

and during maintenance procedures.
CellClean Alkaline detergent used for internal cleaning of the 60 days

SP-50
*See product insert and/or container for specific data.
Analyser components – Smear Production
Smear Production
1. Once the barcode has been read the first step in smear production is for a slide to be removed
from either slide supply cassette (1).
2. The slide is transported to the slide print position where the print unit (2) prints sample information
on the frosted end of the slide.
3. Next, slides are transported to the dispensing and smear position (3). The dispensing pipette
dispense blood evenly over a wide area. The flexible suspension plate prepares the smear by
adjusting the angle and speed of the spreader glass according to the HCT value.
4. Once prepared, the smear is transported via the conveyance block (4) to the staining block.
During this process the smear passes a drier unit where it is dried.
5. Once at the staining block the (depending upon protocol) smear is placed in stain block 1 (5),
followed by stain block 2 (6). Then finally on to the rinse block (7).
6. Once dry, the slides are transported to the magazine feeder unit (8).
7. The prepared slides are placed into an empty magazine and fed out to the magazine storage
unit (9).
Analyser components – Staining Principles
Staining Principles
Romanowsky stains, such as Giemsa, Leishman and May-Grunwald, are used widely for the routine
staining of peripheral blood films. Whichever combination of stains is used the distinction in staining is
achieved due to the balance of basic and acidic components within the stains.
The colour and particular cellular component stains, is dependent on the binding that occurs between
the staining compounds and chemical structures within the cell, as well as interaction between dye
molecules, pH and time.
Cellular Component Staining Principle
Proteins and Nucleic Acids Both bind to the basic component of the stain. This results in a blue-
violet or blue colour.
Haemoglobin Has a high affinity for the more acidic components of the stain
resulting in a red or orange colour.
Eosinophil Granules Eosinophil granules have a basic components which have a strong
affinity for acidic components of the stain resulting in a red or orange
colour.
Basophil Granules Basophil granules contain heparin which are strongly acidic and
therefore have a strong affinity for the basic component resulting in
dark purple colour.
Whichever combination of stains are used the process must be carried out at the correct pH. If the pH
is too acidic the basophilic components appear too pink. If the pH is too alkaline conversely there is over
staining as too much basic stain is taken up.
These reaction are also dependent on time as DNA binds to staining compounds more rapidly than RNA
and Haemoglobin is even slower. As a results incorrect concentration of stains and/or timings will result
in poor stains.
Main Screen – SP-50 – IPU Layout
SP-50 – IPU Layout

Main Screen
Area Function
Toolbar (fixed) Most frequently used functions are displayed here.
Toolbar (Changeable) Displays options that can be used in the currently displayed
screen.
Main area The various options and areas for use within the SP-50 are
displayed here.
Status area Displays the current status of the instrument. In addition, you
can perform operations such as slide preparation and
maintenance.
Status Area – SP-50 – IPU Layout
Status Area
Instrument Status
Green Ready (Analysis or maintenance is possible)
Flashing Green Starting up / Analysis in progress / Maintenance in progress
Orange Sample analysis stopped / not possible. An error has occurred, but the
instrument is still operational.
Flashing Orange Starting up/Preparing a slide/Switching the slide preparation mode/

Shutting down/Sleeping
Red Stopped - Error (without alarm) / initialising system / Analyser ready to

be turned off at the end of shutdown
Flashing Red Stopped - Error(with alarm)
Not Lit Powered OFF
Indicator
The statuses of the instrument, connected reagents and peripheral devices are displayed by icons.
If a reagent or device is not connected, an icon does not appear.
Icon Indication
Touch to display the [Reagent Replacement] dialog box.

Changes to orange when reagent volume is low.
Reagent
Status Changes to red when reagent has run out.
Changes to red if there is a connection error.

Host
Host
Comput
er
Present on analysers connected to the DI-60.
DI-60 Changes to red if there is a connection error.

Status
Changes to red if there is a sampler error.
Sample
r Status
Present on analysers connected to a CF-70.
Changes to red if there is an error occurs.

CF-70
Status
Slide Preparation Mode
Displays the selected slide preparation mode.
Sample Number
Displays a sample number. [>] at the beginning of the sample number indicates that the instrument
is ready to aspirate the next sample. If the sample number is not read, or if it has not been entered
manually, a message is displayed to prompt the input of the number.
Sampler/Manual
Touch to register sample information and slide preparation conditions.

Select to display the [Manual Preparation] dialog box or [Sampler Preparation] dialog box.
The icon displayed varies depending on the selected slide preparation method and settings.
Display Slide Preparation Setting
Sampler Preparation N/A
[Cap Open] is OFF
Manual Preparation
[Cap Open] ON
[RBT] is ON
Error Message
Displays the highest priority error among all current errors. DI-60 & CF-70 (if connected) errors are
also displayed here.
Colour Status
Orange background/black text Caution
Red background/white text Warning
Mode
Touch to select the slide preparation mode.
Status Area – Quick Guide: Maintenance
Quick Guide: Maintenance

Maintenance Key:
Instructions for Use (IFU) recommended maintenance in BLACK
Sysmex recommended maintenance (optional) in BLUE
As part of routine operation, maintenance must be performed on the SP-50 in order to maintain
optimum instrument performance.
IMPORTANT: Failure to follow recommended maintenance protocols can lead to unnecessary

downtime.
Execute maintenance according to the schedule below:
Maintenance task
Daily Maintenance Perform shutdown 1
Wipe stain baths
Weekly Maintenance Clean spreader glass
Cleaning the smear/stain unit
Cleaning the stain pool
Monthly Maintenance Perform shutdown 2
As Required Maintenance Loading glass slides
Replacing spreader glass
Replacing ink ribbon
Replacing rinse
Replacing reagents
Daily Maintenance – Quick Guide: Maintenance
Daily Maintenance
Performing Shutdown 1
Before commencing Shutdown 1 confirm the following:
• Make sure that slide glass has been loaded. Replenish slide glass if not sufficiently loaded.
• Confirm an empty magazine is loaded in the manual magazine holder. Load an empty magazine
if it is not loaded.
• Make sure that there are sufficient reagents available.
• If SP-50 is on the XN track, place the analyser into ‘offline’ mode.
To perform Shutdown 1:
1. From the [Menu] select [Shutdown].
2. The Shutdown dialog box appears and the manual tube holder extends. Select [Shutdown 1].
3. Follow the instructions on the IPU - Place 4mL CELLCLEN AUTO into the tube holder (cap
removed).
4. Press the Manual Analysis Start Switch. The sample tube holder retracts into the analyser and
aspiration begins.
5. Shutdown 1 takes approximately 30 minutes depending upon the settings. The sample holder
will eject forward once the CELLCLEAN AUTO aspiration finishes. Remove and discard
CELLCLEAN AUTO tube once the sample holder ejects the empty tube. When all operations
are finished, the sample holder automatically retracts into the main unit and the instrument shuts
down.
6. Turn the instrument main power OFF using the main power switch located at the top of the
analyser.
Note: If the CELLCLEAN AUTO is not removed before shutdown finishes, a notification that a sample
tube remains in the sample holder will appear at the next startup.
7. Remove the slide glass used for cleaning. The cleaned slide will be stored in the magazine in
the manual magazine holder.
Wipe Stain Baths
1. Moisten a paper towel with methanol/ethanol
2. Carefully wipe along the top of each stain bath to remove any debris present.
IMPORTANT: The wipe down of the top of the staining bath is to only be performed when the SP-50 is
fully shutdown and is switched off to avoid the risk of injury
Performing Start-up
Before stating up the SP-50 check that all consumables are replenished.
To perform start-up:
1. Make sure the main power switch is turned ON. If required, the main power switch can be kept
on at all times.
2. Press the start-up power switch to start up the instrument.
3. The [IPU logon] dialog box will appear. Select the name of the user by touching the name on the
screen. An onboard keyboard appears, enter the required password. Ordinarily the username is
‘admin’ and the password is ‘admin’.
4. Once logged on the analyser will perform a self-check. This is to determine if there are any errors
on the analyser. The self-check takes approximately 20 minutes and performs the following
tasks:
• Initialisation of the mechanical units.

• Rinsing of the hydraulic unit.
• Replenishment of the reagents.
5. Once the self-check is complete the analyser will be ready for use.
Weekly Maintenance – Quick Guide: Maintenance
Weekly Maintenance
Cleaning the Spreader Glass
To perform cleaning of the spreader glass:
1. From the [Menu] select [Maintenance].
2. From the [Maintenance] menu select [Rinse devices].
3. The [Rinse device] dialog box will appear. Make sure the smear unit cover is closed and Select
[Spreader glass rinsing] followed by [OK]. The smear unit will now move towards the front to an
accessible position.
4. Open the slide unit cover and remove the slide supply cassettes. This will enable you to open
the smear unit cover.
IMPORTANT: Be sure to open the all covers until they are locked in place.
5. Rotate the drying fan forward to gain access to the spreader glass.
6. Clean the surface of the spreader glass with gauze moistened with ethanol/methanol. If required,
CELLCLEAN can be used to remove stubborn dirt.
7. Once the clean is complete, restore all components back to their original position. On the IPU
select [OK] to complete task.
Cleaning the Smear/Stain Unit
To perform cleaning of the smear/stain unit:
2. From the [Maintenance] menu select [Rinse devices] the [Cleaning].
3. The [Cleaning] dialog box will appear and the manual tube holder extends. Select [Smear
unit/Stain unit].
removed).
aspiration begins. When finished the holder is ejected forwards and the empty tube can be
removed.
6. The time for cleaning is as followed: [Smear Unit/Stain Unit] approx. 50 minutes.
7. Once complete, press the mode switch on the main unit to retract the tube holder.
Cleaning the Stain Pool
On occasion the staining pool needs to be removed and cleaned to remove dirt or stain deposit. If using
either methanol or ethanol do not soak for longer than 30 minutes as this could damage the staining
pool.
To clean the staining pool:
1. Perform [Shutdown 1] to power down the analyser before cleaning. Instruction of how to do this
can be found in the [Daily Maintenance] section of this workbook.
2. Once the analyser has been shutdown, open the stain unit cover to gain access to the staining
pools.
3. Pull the staining pool cover down and lift and remove the staining pools. Remove both pools,
front & back.
4. Clean the staining pool by soaking it in a rinse solution (Methanol, ethanol or lukewarm water).
Make sure the staining pool is completely dry before placing back into the analyser.
5. Once dry, reinstall the staining pool and restore all affected components back to their original
position.
Monthly Maintenance – Quick Guide: Maintenance
Monthly Maintenance
Performing Shutdown 2
Perform Shutdown 2 once a month or when instrument will not be in use for 1 week or more.
To perform Shutdown 2:
1. From the [Menu] select [Shutdown].
2. The Shutdown dialog box appears and the manual tube holder extends. Select [Shutdown 2].
removed).
aspiration begins.
5. Shutdown 2 takes approximately 30 minutes depending upon the settings. The sample holder
will eject forward once the CELLCLEAN AUTO aspiration finishes. Remove and discard
CELLCLEAN AUTO tube once the sample holder ejects the empty tube. When all operations
are finished, the sample holder automatically retracts into the main unit and the instrument shuts
down.
6. Turn the instrument main power OFF using the main power switch located at the top of the
instrument. Leave the instrument off for at least 2 hours before performing the start-up.
Note: If the CELLCLEAN AUTO is not removed before shutdown finishes, a notification that a sample
tube remains in the sample holder will appear at the next startup.
7. Remove the slide glass used for cleaning. The cleaned slide will be stored in the magazine in
the manual magazine holder.
8. Perform Start-up procedure as per Shutdown 1.
As Required Maintenance – Quick Guide: Maintenance
As Required Maintenance
Loading Glass Slides
To load the glass slides:
1. Open the slide unit cover and remove the slide supply cassettes. Before removing, make sure
that the slide cassette status LED is either Green or red.
2. Remove the slide supply cassette guide and load in the new slides. Load the glass slides so that
the frosted end faces upwards and is positioned toward the ‘guide end’ of the cassette, opposite
end to the handle.
3. Replace the slide supply cassette guide, load the slide cassettes back into the slide supply unit
and close the slide unit cover.
Replace Spreader Glass
To replace the spreader glass:
2. From the [Maintenance] menu select [Replacement].
3. The [Replacement] dialog box will appear. Make sure the smear unit cover is closed and select
[Replace spreader glass]. The smear unit will now move towards the front to an accessible
position.
5. Rotate the drying fan forward to gain access to the spreader glass.
6. Remove the spreader glass from the holder by gently pulling it forward.
7. Set the new spreader glass in the holder. Set the spreader glass so that the edge with the small
beveled edges faces forward. Insert the spreader glass all the way into the spreader glass holder
until it stops.
8. Once the spreader glass has been replaced, restore all affected components back to their
original position. On the IPU, select [OK] on the spreader glass operation count followed by [OK].
This will reset the counter.
Replacing Ink Ribbon
To replace the ink ribbon:
2. Rotate the drying fan forward to gain access to the ink ribbon.
3. Remove the ink ribbon cartridge from the bracket as shown in the diagram below:
(1) Pull the protrusion slide the bracket to the left to move the ribbon to the removal position.
(2) Lift the ribbon and remove the hook pin from the bracket.
(3) Remove the ribbon cartridge.
4. Remove the ink ribbon by lifting both rollers out from the ribbon cartridge.
5. Replace the ink ribbon making sure the ink side of the ribbon faces down. Place the blue roller
on the side with the seal with the white roller on the opposite side.
6. Before loading, wind the white roller in the direction shown to remove in slack in the ribbon.
7. Install the ink ribbon cartridge as shown in the diagram below:
(1) Attach the ribbon to the hook pin

on the bracket.
(2) Slide the bracket back to the
right to load the ink ribbon
cartridge.
8. Once the ink ribbon has been replaced, restore all affected components back to their original
position.
Replacing Reagents – CellPack DCL, Stains & Buffer Solution
To replace the CellPack, Stains and Buffer reagents on the SP-50:
2. From the [Maintenance] menu select [Replacement].
3. The [Replacement] dialog box will appear. Select [Reagent Replacement].
4. The [Reagent Replacement] dialog box will appear. A red warning triangle will be displayed
indicating which reagent requires replacing.
5. The [Reagent Replacement] dialog box displays the reagent information and remaining volume.
Reagent Information
Shelf life after first opening Displays the shelf life of the reagent after it has been opened. This
is not displayed if the reagent has not been registered. When the
shelf life after first opening has expired, it is displayed in white
letter on a red background
Lot No Displays the lot number of the reagent
Remaining volume graph The remaining CELLPACK DCL and concentrated phosphate
buffer reagent appears as a graph.
Reagent state For CELLPACK DCL and concentrated phosphate buffer, the
reagent level is shown. When the reagent runs low, the
background becomes yellow. During CELLPACK
DCL replacement, progress is indicated as "0 to 100 %"
Reagent name Displays the reagent name
6. Input the new reagent information by scanning the barcode on the outer box/container of the
new reagent. You can also enter the reagent code manually by selecting the reagent required
and entering the information using the onboard keypad. If changing manually, make sure the
[Replace the reagent] box is ticked.
7. When the correct reagent information has been entered the red warning triangle will be replaced
by a green tick.
8. Remove the old container and replace it with the new reagent. Select [Execute] to prime the new
reagent. Once complete the [Reagent Replacement] dialog box closes.
Replacing Methanol
To replace the methanol on the SP-50:
1. Remove the cap from the container and discard any residual reagent. When removing the float
switch take care not to touch the end or place it on a dirty surface.
2. Fill the container with Methanol to the desired level and replace the cap and float switch.
3. Display the [Reagent Replacement] dialog box.
4. Select [Methanol] followed by [Execute]. Reagent replenishment will now start, once complete
the [Reagent Replacement dialog box will update.
5. When finished, select [Cancel] to close dialog box.
– Quick Guide: Running Samples
Quick Guide: Running Samples

Sample Preparation
There are 2 methods of preparing a slide on the SP-50, sampler preparation and manual preparation.
Sampler Preparation
Sample tubes with the cap closed are placed in a sample rack and loaded on the instrument. All steps
from sample mixing to smear preparation are performed automatically. You can place up to 50 samples
in the sampler at a time.
Manual Preparation
Use this method for urgent samples or samples that have volume that is not sufficient for the system /
rack mode. Samples must be mixed manually by inversion before analysis.
Mode Sampler Manual Description
✓ ✓ Use this mode when you want to print sample

information and perform sample smear & stain.
[Smear & Stain] Mode

✓ ✓ Use this mode when you want to print sample
information and perform sample smear only.
[Smear] Mode
X ✓ Use this mode when you want to stain a smear

sample that has been manually prepared.
[Staining] Mode
X ✓ Use this mode when you only ant to print sample

information onto a glass slide.
[Print] Mode
Sample Volumes – Quick Guide: Running Samples
Sample Volumes
Sample aspiration volumes and sample volumes required for smear preparation are indicated below:
Slide
Sample Sample Tube Aspiration Required
Preparation Cap [Cap Open]
Tube Position Volume Volume
Method
Regular ✓ Sample rack 500 µl

Sampler N/A 70 µl
RBT ✓ RBT Rack 250 µl
Regular ✓ Regular Tube OFF 70 µl 500 µl

Holder
Regular X ON 38 µl 300 µl
(1 slides)
Regular X 70 µl
(2 slides)
Manual
RBT ✓ OFF 70 µl 250 µl
Micro X Micro Tube N/A 38 µl 110 µl

(1 slide) Holder
Micro X 70 µl 140 µl
(2 slides)
Sampler Mode – Quick Guide: Running Samples
Sampler Mode
To run samples in sampler mode:
1. Make sure the SP-50 is in the ready state. The sample holder should be retracted into the main
unit and the status indicator LED should be green.
2. If required, change the slide preparation mode to the desired setting. Select [Mode] from the
status area to open the [Preparation Mode] dialog box.
3. Select either [Smearing & Staining] or [Smearing] and touch [OK].
Sampler Mode – Quick Guide: Running Samples
4. For standalone analysers; place the rack containing the sample tubes on the right-hand side of
the sampler unit. Slide the groove on the sample rack onto the protrusion, the rack will be
automatically fed into the analyser(s) for processing. A maximum of 5 sample racks can be
loaded at any one time. For tracked analysers, in System mode, racks will automatically be fed
into the SP-50.
5. Once analysis is complete, remove the sample rack from the left-hand side of the sampler unit.
6. [Staining & Smearing] mode: The prepared smears will be stored in the slide magazine. When
preparation of all smears is complete, the magazine containing the samples will be ejected to
the storage location for removal.
7. [Smearing] mode: The smears will be loaded into the manual magazine holder for removal.
Staining & Smearing Mode Smearing Mode
Operation of the SP-50 on the XN Track – Quick Guide: Running Samples
Operation of the SP-50 on the XN Track
Placing into ‘Offline’ or Isolation
The SP-50 should be placed offline when performing any maintenance, running the analyser in ‘Sampler’
mode or troubleshooting on the analyser as this will prevent further racks being sent to the analyser.
To isolate or place the SP-50 offline:
1. Select the [Mode Switch] on the front of the CV-unit of the SP-50
2. The LED indicator light will change from Green to Orange.
3. Carry out the sample preparation, maintenance or troubleshooting required.
4. Once complete select the [Mode Switch] again to put the analyser back into ‘System’ Mode.
Running Samples in ‘Offline’ or Sampler Mode
To run samples in ‘Sampler’ Mode:
1. Isolate the SP-50 from the track (please see above).
2. Place racks on the right hand side of the sampler unit and run like normal.
3. Once complete the rack will be available on the left hand side of the sampler unit.
Manual Mode – Quick Guide: Running Samples
Manual Mode
Manual mode can be used for the following situations:
• When you want to interrupt sampler preparation for an urgent sample.

• When you want to prepare a smear using a micro collection sample tube.
• When you only want to stain an already-prepared smear.
• When you only want to print sample information on the slide glass.
Manual – Smearing & Staining
To perform manual a smear & stain:
1. Make sure the SP-50 is in the manual mode by pressing the mode switch on the front of the main
unit.
2. From the IPU, select [Mode] from the status area. The [Preparation Mode] dialog box will open.
Select [Smearing and staining] followed by [OK].
3. Select [Manual] from the status area, the [Manual Preparation] dialog box will open. Configure
the menu for your sample type.
A) Sample No - Sample number, if required, can be manually entered here. Sample numbers can
be entered either via a hand-held barcode reader or inputted using the onboard keyboard.
B) Set smear – Can be used, if required, to run samples using alternative smearing conditions.
C) Cap open – Select this box to aspirate samples with the cap open loaded into the regular sample
tube holder.
D) RBT – Select this box if using a Raised Bottom Tube (RBT)
E) Read barcode – Select this box if you wish the analyser to read the sample barcode. This
function can only be performed for samples loaded into the regular sample tube holder.
F) Preparation action display area: This area shows the settings for slide preparation actions by
the icons below. When the icon is blue, the setting is ON.
[Alarm] – If selected, an alarm will sound at the end of smear preparation.
[Blood Aspiration Sensor] – Select to enable/disable blood aspiration sensor. Disable sensor for small
volume samples.
[Query to Host] – Select to query the host for the order.
[Additional Rinse Count] – This option allows the user to perform additional rinses for the piercer and
spreader glass if required.
G) Preparation order display area: Displays information of the order. When the icon is blue, the
setting is ON. This are allows the user to select:
• Which slide supply cassette is used

• To send, if connected, to the DI-60
• Number of slides to produce
4. Once the above settings have been confirmed, mix the sample as shown:
5. Place the sample tube in to the correct position in the sample holder.
6. Press the start switch on the front of the main unit. The sample holder will retract and smear
preparation starts. When the sample aspiration is completed the sample holder is ejected out
automatically.
7. Once complete, remove sample and close the sample tube holder by pressing the mode switch
on the front of the main unit.
8. The prepared smears, once complete will be stored in the magazine. The magazine is
automatically ejected to the storage location. Magazines can be manually ejected using the
‘Eject’ switch.
Manual – Stain Only
To perform a stain only:
1. Make sure the SP-50 is in manual mode by pressing the mode switch to eject the sample tube
holder.
2. Check the instrument and magazine holder status LED’s are green.
3. Load the prepared slides into an empty storage magazine. Load the slides so that they face the
same direction with the frosted end of the slide facing towards the front.
4. Open the manual magazine holder cover, pullout the holder and load the magazine. You can
load either sections or both if required. The left hand-side magazine will be processed first.
5. Push the magazine hold back into the analyser and close the magazine holder cover.
6. Select [Mode] from the status area. The [Preparation Mode] dialog box will open. Select
[Staining] followed by [OK].
7. From the status area, select the [Manual] option if you wish to alter the staining protocol. E.g. for
bone marrow staining.
8. Press the start switch on the front of the main unit.
9. Once complete, close the sample tube holder by pressing the mode switch on the front of the
main unit.
10. The prepared smears, once complete will be stored in the magazine. The magazine is
automatically ejected to the storage location. Magazines can be manually ejected using the
‘Eject’ switch.
Manual – Smear Only
To perform a smear only:
holder.
2. Check the status indicator LED of the instrument and magazine holders are green. Load an
empty magazine into the manual magazine holder.
3. Select [Mode] from the status area. The [Preparation Mode] dialog box will open. Select
[Smearing] followed by [OK].
Note: Details for this can be found in the ► [Manual – Smearing & Staining] section.
5. Once the above settings have been confirmed, mix the sample as shown:
Place the sample tube in to the correct position in the sample holder.
Press the start switch on the front of the main unit. The sample holder will retract and smear preparation
starts. When the sample aspiration is completed the sample holder is ejected out automatically.
Once complete, remove sample and close the sample tube holder by pressing the mode switch on the
front of the main unit.
The prepared smears, once complete will be fed out into the manual magazine holder.
Manual – Print Only
To perform a print only:
holder.
2. Check the status indicator LED of the instrument and magazine holders are green. Load an
empty magazine into the manual magazine holder.
3. Select [Mode] from the status area. The [Preparation Mode] dialog box will open. Select [Print]
followed by [OK].
Note: Details for this can be found in the ► [Manual – Smearing & Staining] section.
5. Press the start switch on the front of the main unit.
6. Once complete, close the sample tube holder by pressing the mode switch on the front of the
main unit.
7. The printed slides, once complete will be stored in the manual magazine holder.
Responding to Alarms – Quick Guide: Running Samples
Responding to Alarms
When an error occurs an audible alarm will be heard and the [Help] dialog box will be displayed on the
IPU. If connected, DI-60 and CF-70 errors will also be displayed here.
If an error occurs while another dialog box is displayed, the error message will appear on the status area
at the bottom of the screen. Close the displayed dialog box and touch the error message on the status
area to display the [Help] dialog box.
Message Type Description
Error message list Displays the current errors. Errors that have the highest priority
are displayed at the top.
Action message Displays troubleshooting action for the selected error.
In the [Help] dialog box screen you can perform the following functions:
• Stop the alarm.

• Access the IFU (Instructions For Use).
• Execute and/or accept the error. [Execute] will execute the action required, [Accept] will clear
the error.
Audit Logs – Quick Guide: Running Samples
Audit Logs
The SP-50 has the following audit logs which can be viewed:
Logs Description
Audit log Displays a log of operations.
Error log
Displays a log of errors that have occurred and information at the time of
occurrence and clearance.
Maintenance log
Displays a log of maintenance tasks executed and information at the time of
execution.
Reagent
replacement log Displays a log of reagent replacement and any information that was entered at
the time of replacement.
A maximum of 5,000 entries are saved in each log. When the maximum number of entries is reached in
a log, each new entry automatically deletes the oldest entry.
To check a log:
1. From the [Menu] screen select [Log].
2. The [Log] menu screen will open. Select the desired log you wish to view.
To add a comment to a log:
1. Once in the desired log, select [Comment] from the top toolbar.
2. Enter a comment up to a maximum of 100 characters.
3. Select [OK]. The comment is now added.

To save a log in CSV format:
1. From the [Menu] screen select [Log].
2. Select the desired log you wish to save.
3. Insert the USB stick into a free USB port on the back of the instrument.
NOTE: A USB memory stick with a password lock function cannot be used.
4. Select [File] from the top toolbar and select [Output in CSV Format]. The save starts and the
[Waiting for completion] dialog box appears. When the dialog box closes, the save is complete.
When performing a save, the file location/folder/name is automatically created on the memory
stick.
5. Remove the USB stick.
Mail – Contact Us
Contact Us
Mail
Training Academy
Sysmex UK Ltd
Garamonde Drive
Wymbush
Milton Keynes
MK8 8DF
Phone
Product Hotline
For urgent application support 0333 320 3466 (UK)
Service Hotline
For technical support and service team 0333 320 3467 (UK)
Reagent ordering 0333 320 3470 (UK)
Email
Product mail (non-urgent) [email protected]

Training Academy (non-urgent) [email protected]
Jennifer Hammersley [email protected]
Karl Elgerton [email protected]
Natalie Bowen [email protected]

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Replacing Ink Ribbon 39

Revised section Alteration Name Date

Weekly maintenance Amended weekly schedule K. Elgerton January 2019

Reagents Amended changing stain K. Elgerton January 2019

Front cover New logo added K. Elgerton January 2020

Contacts Reagent hotline updated K. Elgerton January 2020

Document title Version Date

SP-50_BO (IFU) 1801 January 2018

SP-50_TS (IFU) 1801 January 2018

Throughput SP-50 S – 30 samples/hr

Capacity Standalone analyser – 50 samples

Sample Type Whole blood

Sampler Mode Regular tube

Manual Mode Manual (Closed)

Aspiration Volumes Sampler mode - 70µl

Staining Protocols May-Grunwald-Giemsa stain

3. Start-up (Power) Switch – Press to start up the instrument.

4. Eject Switch – Press to eject magazines from the stain unit.

5. IPU Touchscreen – Use to operate the instrument and configure settings.

6. Sample Tube Holder – Used to set samples for manual analysis.

• When the sample holder is open: Manual preparation

8. Start Switch – Press to start manual analysis.

9. Status Indicator LED - Shows the current status of the analyser.

LED Indicator Light Description

Green Ready (Analysis or maintenance is possible)

Flashing Green Starting up / Analysis in progress / Maintenance in progress

Red Stopped - Error (without alarm) / initialising system / Analyser ready to be

Flashing Red Stopped - Error(with alarm)

Not Lit Powered OFF

LED Indicator Light Description

Green Containing magazine

Not Lit Powered OFF

1. 0.07 MPa Regulator – Regulates the source pressure to 0.07 MPa

LED Indicator Light Description

Green Slides loaded

Not Lit Powered OFF

3. Smear Dryer Unit – Dries the smeared sample.

Stain Pool Role Reagent

5 Staining Stain 2/buffer

*Use depends upon protocol

Reagents On-board the SP-50

Reagent Name Function On-board Stability

May-Grunwald stain Used for staining blood smears. N/A*

Giemsa stain Used for staining blood smears. N/A*

Buffer solution pH 7.0 Used in the preparation of blood smears. N/A*

Methanol Used in staining process depending on protocol N/A*

CellClean Alkaline detergent used for internal cleaning of the 60 days

*See product insert and/or container for specific data.

Cellular Component Staining Principle

SP-50 – IPU Layout

Toolbar (fixed) Most frequently used functions are displayed here.

LED Indicator Light Description

Green Ready (Analysis or maintenance is possible)

Flashing Green Starting up / Analysis in progress / Maintenance in progress

Flashing Orange Starting up/Preparing a slide/Switching the slide preparation mode/

Red Stopped - Error (without alarm) / initialising system / Analyser ready to

Flashing Red Stopped - Error(with alarm)

Not Lit Powered OFF