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    Liquid Chromatograph Mass Spectrometer

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    Liquid Chromatograph Mass Spectrometer

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    Instrumentation Tools
    9 views4 pages
    This document provides information about instrumentation tools and topics covered on the website https://instrumentationtools.com. The website teaches instrumentation and control engineering, covering industrial instrumentation, PLCs, DCS, SCADA, field instruments, analyzers and other topics.

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    This document provides information about instrumentation tools and topics covered on the website https://instrumentationtools.com. The website teaches instrumentation and control engineering, covering industrial instrumentation, PLCs, DCS, SCADA, field instruments, analyzers and other topics.

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    © All Rights Reserved
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    9 views4 pages

    Liquid Chromatograph Mass Spectrometer

    Uploaded by

    Instrumentation Tools
    This document provides information about instrumentation tools and topics covered on the website https://instrumentationtools.com. The website teaches instrumentation and control engineering, covering industrial instrumentation, PLCs, DCS, SCADA, field instruments, analyzers and other topics.

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    Instrumentation Tools

    Learn Instrumentation and Control Engineering. Inst Tools covers topics of Industrial
    Instrumentation, PLC, DCS, SCADA, Field Instruments, Analyzers and so on.
    https://instrumentationtools.com

    Author: userr

    Categories: Multiple Choice Questions

    Liquid Chromatograph- Mass Spectrometer

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    Liquid Chromatograph- Mass Spectrometer

    1. Introduction of total HPLC effluent into MS is feasible.

    a) True
    b) False

    Answer: b

    Explanation: Introduction of total HPLC effluent into MS is not feasible. The simplest method is
    the introduction of liquid from LC to the MS ion source region.

    2. The gas burden from conventional LC flow rates creates nearly _____ times more gas
    than cryo-pumped vacuum system can handle.

    a) 5
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    b) 10
    c) 20
    d) 40

    Answer: c

    Explanation: The gas burden from conventional LC flow rates creates nearly 20 times more
    gas than cryo-pumped vacuum system can handle.

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    .c
    3. Which of the following should be in position to split the effluent?

    s
    a) Interface

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    b) Ion source
    c) Makeup gas To
    d) Microbore
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    Answer: a
    at

    Explanation: Interface should be in position to split the effluent. LC-MS is a very advantageous
    nt

    method.
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    4. Which of the following is the most commonly used interface?


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    a) Nebulizer
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    b) Chopper
    In

    c) Filter
    d) Vapourising chamber

    Answer: a

    Explanation: Nebulizer is the most commonly used interface. The nebulizer gas and the make-
    up gas are introduced coaxially into the heated nebulization region.

    5. Only ______ percent of the effluent of the liquid chromatography must be introduced
    in the mass spectrometer.

    a) 1-2
    b) 1-5
    c) 1-20
    b) 1-15

    Answer: b

    Explanation: Only 1-5 percent of the effluent of the liquid chromatography must be introduced
    in the mass spectrometer. Therefore, the total effluent must be split.
    InstrumentationTools.com
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    Instrumentation, PLC, DCS, SCADA, Field Instruments, Analyzers and so on.
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    6. Which of the following is the normal nebuliser temperature used in LC mass


    spectrometry?

    a) 50-100oC
    b) 100-200oC
    c) 125-150oC
    d) 150-200oC

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    Answer: c

    .c
    Explanation: 125-150oC is the normal nebuliser temperature used in LC mass spectrometry. It

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    is suitable for a variety of applications.

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    7. The ions are focussed and de-clustered through which of the following regions?
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    a) Dry helium region


    b) Wet oxygen region
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    c) Wet chlorine region


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    d) Dry nitrogen region


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    Answer: d
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    Explanation: The ions are focussed and de-clustered through a dry nitrogen region. It is then
    sent into the high vacuum analyser.

    8. The ions are passed into the high vacuum analyser through which of the following?

    a) Orifice
    b) Nozzle
    c) Nebulizer
    d) Venturi tube

    Answer: a

    Explanation: The ions are sent into the high vacuum analyser through an orifice. It is then
    mass analysed.

    9. Using an interface would reduce detection limit and sensitivity.

    a) True
    b) False

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    Answer: a

    Explanation: The total effluent must be split. Using an interface would reduce detection limit
    and sensitivity.

    10. Gas burden from conventional LC flow rates is which of the following?

    a) 1ml/min of water produces 1.2 l/mm of gas


    b) 1ml/min of water produces 2.4 l/mm of gas

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    c) 2ml/min of water produces 3.2 l/mm of gas

    .c
    d) 1ml/min of water produces 4.2 l/mm of gas

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    Answer: a
    To
    Explanation: In LC-mass spectrometry, 1ml/min of water produces 1.2 l/mm of gas. This is the
    on
    gas burden that conventional flow rates produced.
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