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    Antibody Human Active BMP 7

    Uploaded by

    made82
    This product listing provides details about a human active BMP-7 recombinant protein sold by Thermo Fisher Scientific. The protein is a homodimer containing two 117 amino acid subunits of human BMP-7 expressed in CHO cells. It has a molecular weight of 28.8 kDa and is 98% pure by SDS-PAGE and HPLC analysis with less than 0.1 ng/μg of endotoxin. The protein has an ED50 of 0.02-0.04 ng/mL in inducing alkaline phosphatase activity in mouse chondrogenic cells.

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    Antibody Human Active BMP 7

    Uploaded by

    made82
    2 views2 pages
    This product listing provides details about a human active BMP-7 recombinant protein sold by Thermo Fisher Scientific. The protein is a homodimer containing two 117 amino acid subunits of human BMP-7 expressed in CHO cells. It has a molecular weight of 28.8 kDa and is 98% pure by SDS-PAGE and HPLC analysis with less than 0.1 ng/μg of endotoxin. The protein has an ED50 of 0.02-0.04 ng/mL in inducing alkaline phosphatase activity in mouse chondrogenic cells.

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    This product listing provides details about a human active BMP-7 recombinant protein sold by Thermo Fisher Scientific. The protein is a homodimer containing two 117 amino acid subunits of human BMP-7 expressed in CHO cells. It has a molecular weight of 28.8 kDa and is 98% pure by SDS-PAGE and HPLC analysis with less than 0.1 ng/μg of endotoxin. The protein has an ED50 of 0.02-0.04 ng/mL in inducing alkaline phosphatase activity in mouse chondrogenic cells.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    2 views2 pages

    Antibody Human Active BMP 7

    Uploaded by

    made82
    This product listing provides details about a human active BMP-7 recombinant protein sold by Thermo Fisher Scientific. The protein is a homodimer containing two 117 amino acid subunits of human BMP-7 expressed in CHO cells. It has a molecular weight of 28.8 kDa and is 98% pure by SDS-PAGE and HPLC analysis with less than 0.1 ng/μg of endotoxin. The protein has an ED50 of 0.02-0.04 ng/mL in inducing alkaline phosphatase activity in mouse chondrogenic cells.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Catalog # PHC7204

    Human Active BMP-7 Recombinant Protein


    Product Details
    Size 10 µg
    Species Human
    Expression system CHO cells
    Amino acid sequence Human BMP-7 recombinant protein is a homodimer containing two 117 amino acid subunits (BMP-7,
    amino acids 315-431)
    Molecular weight 28.8 kDa
    Class Recombinant
    Type Protein
    Purity 98% by SDS-PAGE and HPLC
    Endotoxin concentration <0.1 ng/µg
    Activity ED50 = 0.02 - 0.04 ng/mL; determined by the dose-dependent induction of alkaline phosphatase in
    mouse ATDC52 chondrogenic cells.
    Conjugate Unconjugated
    Form Lyophilized
    Purification sequential chromatography
    Contains no preservative
    Storage conditions -20°C

    Applications Tested Dilution Publications


    Western Blot (WB) Assay-dependent -
    Immunohistochemistry (IHC) Assay-dependent -
    ELISA (ELISA) Assay-dependent -
    Functional Assay (FN) Assay-dependent -
    Control (Ctrl) Assay-dependent -

    Product Specific Information


    Carrier-Free

    Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in
    sterile, distilled water, to a concentration of 0.1-1.0 mg/mL (Do not vortex) and store at 2-8°C for up to 1 week. Make any further
    dilutions of the reconstituted protein in buffer containing carrier protein, such as 0.1% BSA, and apportion into working aliquots to
    store at -20°C to -80°C.

    Storage: The lyophilized protein is stable at room temperature for 1 month. Store the lyophilized protein at -20°C to -80°C,
    preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. Working aliquots stored with a
    carrier protein are stable for at least 7 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

    Support (US): 1 800 955 6288


    Support (Outside US): thermofisher.com/contactus 1
    E-mail: [email protected]
    For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation,
    specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to
    one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer is merely illustrative of the general type and quality of goods and does not represent that any
    Product will conform to such model or sample. NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY
    FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT
    OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise
    expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type
    of consumption by or application to human or animals.

    Support (US): 1 800 955 6288


    Support (Outside US): thermofisher.com/contactus 2
    E-mail: [email protected]

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