Biodiversity

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Group 1

1.1 and 1.2

1.1

a) State types of biodiversity

There are three distinct types of biodiversity: genetic diversity, species diversity, and
ecosystem diversity.

b) State the hierarchical classification

Hierarchical classification involves grouping and categorizing organisms into


taxonomic groups based on their biological characteristics.

c) Explain briefly the three-domain system

The three-domain system, proposed by Carl Woese in 1977, is based on molecular


data revealing significant differences in ribosomal RNA sequences. This system
classifies microorganisms into three domains—Domain Bacteria, Domain Archaea,
and Domain Eukarya—at a taxonomic level higher than the kingdom level.

1.2: Domain Bacteria and Domain Archaea

a) State the two domains of prokaryotes

Prokaryotes are classified into two domains: Domain Bacteria and Domain Archaea.
For instance, E. coli represents Domain Bacteria, while Sulfolobus sp. represents
Domain Archaea.

b) Differentiate between the two prokaryotic domains, Bacteria and Archaea, based
on:

i. Cell wall structure

ii. Association of histones with DNA

iii. Structure of membrane lipids

i. Domain Bacteria's cell wall contains peptidoglycan, while Domain Archaea


lacks peptidoglycan.
ii. DNA in Domain Bacteria does not associate with histone protein, while
DNA in Domain Archaea is associated with histone protein.
iii. iii. Domain Bacteria's membrane lipids consist of unbranched hydrocarbon
chains connected to glycerol by ester linkage, whereas Domain Archaea's
membrane lipids consist of branched hydrocarbon chains connected to
glycerol by ether linkage.

b) Describe the diversity of bacteria

i) Cell shape (coccus, bacillus, spirillum, and vibrio)

a) Coccus: Spherical bacteria examples include Staphylococcus aureus and


Streptococcus pneumoniae.

b) Bacillus: Rod-shaped bacteria examples include Escherichia coli (E. coli) and
Bacillus anthracis.

c) Spirillum: Spiral-shaped bacteria with examples such as Spirillum volutans.

d) Vibrio: Comma-shaped bacteria, with Vibrio cholerae as an example.

ii) Gram-stain (Gram-positive, Gram-negative)

a) Gram-Positive Bacteria:
These bacteria, such as Staphylococcus aureus and Streptococcus pyogenes,
retain the crystal violet stain, appearing purple or blue under a microscope
due to a thick peptidoglycan layer.
b) Gram-Negative Bacteria:
Examples like E. coli and Salmonella sp. take up the safranin counterstain,
giving them a pink or red color due to a thinner peptidoglycan layer and the
presence of an outer membrane.

d) Explain the roles of bacteria

i) Recycling of chemical elements in the ecosystem Bacteria, such as Rhizobium sp.,


participate in nitrogen fixation, converting nitrogen into forms usable by other
organisms.

ii) Symbiotic relationships (e.g., E. coli in the human intestine) Enterobacteria like E.
coli live symbiotically in the human intestine, providing essential functions like
producing biotin and vitamin K for the host.
iii) Pathogenic bacteria (e.g., Salmonella sp.) Pathogenic bacteria, like Salmonella sp.,
can cause various diseases in animals and plants, such as food poisoning and
tetanus.

iv) Contributions in research and technology (e.g., bacterial plasmid) Bacterial


plasmids, like those used in DNA cloning vectors, play a significant role in research
and biotechnology by carrying target genes into host cells.

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