Scribd Logo
Upload

Welcome to Scribd!

  • 5 views

    Isolation of Aerotolerant Campylobacter

    Uploaded by

    kamalikau
    Isolation of Arcobacter spp. (aerotolerant Campylobacter) from Chaetocerous algae culture in a shrimp hatchery in Chilaw, Sri Lanka

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    Isolation of Aerotolerant Campylobacter

    Uploaded by

    kamalikau
    5 views3 pages
    Isolation of Arcobacter spp. (aerotolerant Campylobacter) from Chaetocerous algae culture in a shrimp hatchery in Chilaw, Sri Lanka

    Original Title

    Isolation of aerotolerant Campylobacter

    Copyright

    © © All Rights Reserved

    Available Formats

    DOC, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Isolation of Arcobacter spp. (aerotolerant Campylobacter) from Chaetocerous algae culture in a shrimp hatchery in Chilaw, Sri Lanka

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    5 views3 pages

    Isolation of Aerotolerant Campylobacter

    Uploaded by

    kamalikau
    Isolation of Arcobacter spp. (aerotolerant Campylobacter) from Chaetocerous algae culture in a shrimp hatchery in Chilaw, Sri Lanka

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 3

    Isolation of Arcobacter spp.

    (aerotolerant Campylobacter) from Chaetocerous algae


    culture in a shrimp hatchery in Chilaw, Sri Lanka
    Considering primarily ease of growth and sufficient omega-3 unsaturated fatty acids
    profiles, Chaetocerous phytoplankton is frequently used in large quantities as a larval
    shrimp feed in commercial shrimp culturing in Sri Lanka. Use of new salt water, reliable
    phytoplankton starter culture and fresh culture containers are of paramount importance
    since bacteria can overtake the phytoplankton cultures. Arcobacter is closely related to
    Campylobacter and has recently gained attention as an emerging food and waterborne
    pathogen of human and potential pathogen of animals (Ho et al., 2006). There is evidence
    that livestock animals might be a significant reservoir of Arcobacter spp. and isolation of
    this spp. from drinking water reservoir in Germany, from canal water in Thailand, from
    river water in Italy has been reported (Fera et al., 2004). However the number of
    outbreaks and incidents attributable to Arcobacter infection is unknown as this organism
    is not usually included in routine clinical investigations. The isolation of Arcobacter spp.
    from Chaetocerous algae culture was performed according to the traditional culture
    methods. The Chaetocerous algae culture was plated onto sheep blood agar and
    MacConkey agar plate as the standard medium inoculated for the isolation of facultative
    microorganisms at 37oC. The pure cultures were tested Gram staining, motility, oxidase
    and catalase production, oxidative or fermentative glucose metabolism, gas production,
    nitrate reduction and indole production. Further investigation carried out to rule out the
    Campylobacter by culturing onto selective media (Karmali agar) at 42 oC with the
    supplementation of optimal oxygen concentration for growth 5-10%. The antibiotic
    sensitivity test depicted the organism vary in sensitivity to antibiotics; high level of
    sensitivity observed for Enrofloxacin, Streptomycin, Doxycycline whereas resistance was
    observed for Amoxycillin and Tetracycline. Arcobacter species are common human and
    animal pathogens. The association of these organisms with enteritis in human and
    animals indicates their potential public health importance. It was evident Arcobacter spp.
    are members of the bacterial flora of seawater and are associated with zooplankton in the
    coastal environment (Fera et al., 2004). Therefore it is very important to have good
    sanitization in algae culture to success the larval feed, the basic nutritional building block
    of our home shrimp breeding effort.
    Isolation of Arcobacter spp. (aerotolerant Campylobacter) from Chaetocerous algae
    culture in a shrimp hatchery in Chilaw, Sri Lanka

    J.K.H.Ubeyratnea, M.Somaratnea

    a
    Veterinary Research Institute, Gannoruwa, Peradeniya, Sri Lanka
    Dr. J.K.H.Ubeyratne
    Veterinary Investigation Centre
    Veterinary Research Institute
    Gannoruwa
    Peradeniya
    02.04.2008

    Prof. R. Sivakanesan
    Dept.of Biochemistry
    Faculty of Medicine
    Peradeniya

    Abstract for poster presentation

    Herewith I am sending the abstract of the study topic Isolation of Arcobacter spp.
    (aerotolerant Campylobacter) from Chaetocerous algae culture in a shrimp hatchery
    in Chilaw, Sri Lanka for the poster presentation at the Sri Lanka Veterinary Association
    60th Annual Scientific Sessions 2008.

    Thanking you

    ………………………………
    Dr. J.K.H. Ubeyratne

    You might also like