CSIRO PUBLISHING

www.publish.csiro.au/journals/ajea Australian Journal of Experimental Agriculture, 2006, 46, 777–782

Effects of dietary fibre and feeding frequency on wool biting and aggressive
behaviours in housed Merino sheep

S. VasseurA,B , D. R. PaullA , S. J. AtkinsonA , I. G. ColditzA and A. D. FisherA,C

A CSIRO Livestock Industries, FD McMaster Laboratory Chiswick, Locked Bag 1, Armidale, NSW 2350, Australia.
B INA P-G, Institut National Agronomique de Paris-Grignon, 16 rue Claude Bernard, 75 231 Paris Cédex 05, France.
C Corresponding author. Email: andrew.fi[email protected]

Abstract. Wool biting is a behaviour that can develop in housed sheep, in which sheep start to bite and eat the
wool of others. The aim of this study was to determine whether (i) supplementing the diet of housed sheep with
fibre and (ii) increasing feeding frequency would help to reduce wool biting, aggressive behaviours and wool
damage. In a 2 × 2 factorial experiment, 40 Merino wethers were either fed with lucerne-based pellets only, or
with pellets supplemented with barley straw. They received their pellets either on a low feeding frequency basis
(once a day Monday to Friday mornings, double ration on Friday afternoon, nothing Saturdays and Sundays), or
on a high feeding frequency basis (twice a day, every day). The sheep were housed in 4 treatment pens, each with
10 animals. Wool biting and aggressive behaviours were recorded through direct observation and the sheep were
scored for wool damage twice a week during the 15-week study. The provision of fibre had a significant effect on
reducing wool biting (P<0.001) and wool damage score (P<0.001). There was no consistent effect of feeding
frequency on wool biting or wool damage, and no fibre × feeding frequency interactions. Whereas wool biting in
general increased with time during the study (P<0.001), levels of aggressive behaviour showed no consistent time
trend, and there were no effects of fibre or feeding frequency treatments. It is concluded that wool biting is largely
a redirected behaviour in concentrate-fed housed sheep deprived of adequate levels of activity or oral stimulus, and
that the provision of roughage will reduce the development of wool biting and improve animal welfare in housed
experimental sheep.

Introduction evidence from animal house managers suggests that such

Housed sheep sometimes develop a behaviour known as strategies may only delay the appearance of the phenomenon
‘wool biting’, in which 1 or more sheep pick and bite at (Bradley, personal communication).
the wool of other individuals within the group (Lynch et al. Another theory is that wool biting could result from
1992; Sambraus 1985). Wool biting is unpredictable and can an inadequate diet, either in terms of quality or quantity
develop within a few days of housing or after a longer period. (Sambraus 1985). The effects of a reduced volume of food
The behaviour often starts with a few animals biting a small intake on increased oral stereotypic behaviours have been
number of targeted sheep. Once started, however, it appears demonstrated in other species, especially pigs (Bergeron et al.
to spread, and sheep that were not initially involved in wool 2000). This may be partly driven by a need for gut fill, as
biting can adopt the behaviour. Anecdotal evidence would evidenced by a greater water intake in feed-restricted pigs
suggest that, once established, wool biting appears relatively (Barber et al. 1991). The use of concentrated feed in housed
intractable, and isolating the most active wool biters from the sheep may have the same effect on the sensation of gut fill
pen may relieve the practice for only a short period. and lead to the appearance of abnormal oral behaviours such
Wool biting has not been studied in detail and its as wool biting.
causative factors remain a source of speculation. A lack of The use of concentrated feed for housed sheep may also
environmental stimulation and dietary factors are thought to be responsible for a lack of oral stimulation and rumination.
be possible contributing factors (Lynch et al. 1992). Boredom The time spent eating such feed is short and this rapid
could be 1 cause for wool biting, and some techniques of consumption might not provide enough oral stimulation to
environmental enrichment have been developed to reduce the sheep. At pasture, grazing requires extensive use of the
it, including hanging chains from the ceiling, introducing tongue and mouth, but in captivity the opportunities to do
basketballs, plastic bottles or chewing bars in the pens, or so and the substrates upon which to develop this activity
playing music. Effects of these environmental stimuli on are often limited. Any oral frustration that may result could
wool biting have not been formally recorded, but anecdotal contribute to the development of redirected oral behaviours

© CSIRO 2006 10.1071/EA05320 0816-1089/07/060777

778 Australian Journal of Experimental Agriculture S. Vasseur et al.

such as licking water troughs, chewing pen divisions and Table 1. Composition of the two dietary treatments
wool biting. In addition, ruminating is a major component Sheep were fed either lucerne-based concentrate pellets only (NS),
of the sheep ethogram under most conditions, and the use or the same pellets supplemented with barley straw (S)
of concentrated feed with minimal roughage reduces this
behaviour. Campion and Leek (1996) showed that the removal Components (per animal) NS S
of hay from the diet of hay and concentrate-fed sheep reduced Lucerne pellets (g) 700 650
true rumination to zero after a period of 8 days. Barley straw (g) — 300
The rapid consumption of food and the absence of Dry matter (g) 632 717
rumination also result in large amounts of free time for the Crude protein (g) 137 131
sheep. Under Australian conditions, sheep graze for 6 to 9 h Metabolisable energy (MJ) 7.3 7.6
per day (Arnold and Dudzinski 1978; Dudzinski and Arnold
1979). A study recording rumination times of sheep on a
variety of grazed forages found that the animals ruminated Samples of the feeds as offered during the experiment were collected and
for up to 6 h per day (Lofgreen et al. 1957). The reduction of analysed, and these results are presented in Table 1. All the pellets were
rumination time to zero and feeding time to 1 or 2 h potentially consumed each day, however there was approximately 50% wastage of
the straw by the sheep.
liberates 13 to 14 h of time for an animal which, when housed The feeding frequency treatments only concerned the pellets. The
in conventional pens, has a relatively restricted array of LFF groups were fed once a day (0800 hours) during week days and were
environmental interactions. Long periods without feed may given a double ration on Friday afternoon (1600 hours) for the weekend.
also increase boredom. In horses, a study by Cooper et al. Sheep on the HFF treatment were fed twice a day (0800 and 1600 hours)
(2005) suggested that dividing stabled animals’ concentrate with half the total daily amount offered at each occasion, 7 days
per week.
ration into a number of smaller meals may be an effective Each treatment group of 10 sheep was housed in a 2.5 by 5 m pen
means of reducing oral stereotypies. Therefore, the increase with a steel mesh floor and containing 1 drinking trough, 2 feed troughs
in free time and the reduction in oral stimulation which and 1 hay rack. The hay rack was used to deliver the barley straw
result from reduced fibre in the diet could well lead to the (treatment S) or remained empty (treatment NS). The steel mesh on the
development of abnormal oral behaviours in sheep and be a hay rack was 5 by 5 cm. Shade cloth was placed on each panel between
pens to visually separate sheep from adjoining treatment pens.
cause for wool biting. On week 2 of the study, a break in the wool became apparent for
Accordingly, this experiment examines the effect of one sheep in the control group (NS and LFF). This animal was replaced
increased dietary fibre on wool biting, in order to determine by another animal from the same original group that was housed in
whether using more fibre in the diet can prevent or reduce the animal house for 1 week and then transferred to the treatment pen
wool biting, and thereby enhance animal welfare in housed on week 4. The replacement animal had received the same preparatory
feeding regimen prior to entering the experiment.
experimental sheep. The effect of feeding frequency was
also examined, as increasing feeding frequency could help Measurements
The sheep were weighed at the beginning and at the end of the experiment
to reduce boredom in housed sheep and thereby reduce
(week 15). Animal behaviour was recorded by direct observation. Each
wool biting. pen was observed for 1 hour twice a week, once in the morning (0900
to 1000 hours), and once in the afternoon (1500 to 1600 hours).
Materials and methods The following behaviours were recorded: wool biting, aggression,
Animals and treatments horn biting, ear licking and mounting. For each of these events, the
The protocol and conduct of the study were approved by the institutional agonist and recipient sheep were identified and recorded. Wool biting
Animal Ethics Committee, under the NSW Animal Research Act 1985. was defined as a sheep closing its mouth on another animal’s wool
The study utilised 40, 5-month-old Merino wethers from the same and biting or tugging. Aggressive interactions included butts, agonistic
flock. The sheep were brought from the paddocks to the animal house jumping and kicks. All the observations were conducted by the same
1 week before the beginning of the 15-week study, and were individually observer, except for weeks 9, 12 and 13, when a second, trained observer
identified by dye-based numbers branded on their flanks. After a week was utilised.
of acclimation to the animal house environment, the sheep were blocked To quantify the impact of wool biting, a measurement scale was used
by weight, and within block, allocated at random to their treatments. to assess the state of the wool and its damage. The scale consisted of
The experiment utilised a 2 × 2 factorial plan, with 2 levels of 6 levels from 0 to 5:
roughage provision and 2 feeding frequencies. The sheep were either 0: no sign of wool biting on any part of the sheep and no apparent
fed a diet of lucerne-based concentrate pellets only (NS), or the same damage of the wool;
pellets supplemented with barley straw (S). The animals received their 1: wool became fluffed — appearance of small tufts of wool protruding
concentrate pellets on a low frequency basis (LFF) or on a high frequency above the surface of the fleece — score 1 from the first tuft;
basis (HFF). 2: most parts of the fleece contain protruding tufts — high density of
Sheep fed the NS diet received 700 g of pellets a day, whereas sheep these tufts;
on the S diet were offered 650 g of pellets plus 300 g of barley straw 3: high density of tufts + appearance of a brighter area due to confluence
per day (Table 1). The pellets were made to a formulation designed to of protruding tufts or thinning of the fleece leading to greater
supply all the nutrient requirements of maintenance and growing sheep visibility of clean wool below the fleece tip (often on the rump or
(11.6 MJ ME, 21.6% crude protein, and sufficient levels of calcium, legs) — skin was not visible and the bright area was less than 50%
phosphorus, magnesium and sulphur). All feeding was on a group basis. of body surface;
The feeding levels were determined from existing analyses of the feeds, 4: spreading of the brighter area to more than 50% of body surface OR
and were calculated to provide the same levels of energy and protein. skin visible in this brighter area;
Wool biting in housed sheep Australian Journal of Experimental Agriculture 779

5: spreading of the white area to 80–100% of body surface OR skin the mean number of wool biting events per hour for each
visible on more than 10–20% of body surface. At this stage, decisions sheep over the duration of the study, both as attacker and
were made to avoid any further damage (removal of the animal, victim, and indicates that some sheep were more commonly
covering with a sheep coat, etc.).
The scale was objectively recorded using a series of photographs filling 1 role compared with the other. All subsequent results
corresponding to each level. All 40 sheep were scored twice a week, are presented based on the analyses of wool biting and
throughout the study. All the scoring was done by the same observer, aggressive events recorded for the attacking sheep. The
except for weeks 12 and 13, when a trained, second scorer was used. time trends for wool biting events for the main treatment
Statistical analyses effects are presented in Figure 3. There was a treatment
The statistical analyses were performed using Genstat, 7th Edition effect of fibre provision (P<0.001), but not for feeding
(Payne et al. 2003), with individual sheep as the experimental unit. frequency (P>0.05), however, there were treatment × time
Behavioural observation data were analysed by split–split plot ANOVA,
effects for both fibre provision (P<0.001) and feeding
with the main plot including the effects of fibre content, feeding
frequency and their interaction. The 2 subplots included: (i) the frequency (P<0.001). The key finding was that as the study
effect of time of day (morning or afternoon) and its interaction with progressed, the S sheep displayed significantly less wool
fibre content, feeding frequency and fibre content × feeding frequency; biting than the NS sheep. The interaction of fibre provision
and (ii) the effect of week (time repetitions) and its interaction with and feeding frequency was not significant (P = 0.34). The
fibre content, feeding frequency and fibre content × feeding frequency,
effect of time of day was significant (P<0.01), with more
as well as the interaction between time of day and week and the
interaction of this term with fibre content, feeding frequency and fibre wool biting events recorded in the afternoon.
content × feeding frequency. Unlike wool biting, aggressive behaviours were present
Logarithmic transformations of the behavioural data were used. throughout the experiment (Fig. 4), and remained relatively
Because there was insufficient frequency of occurrences of horn biting,
ear biting and mounting to permit robust analysis, these variables were
not analysed. Thus, the variables analysed were: log(number of wool As aggressor As victim
biting events as attacker + 1), log(number of wool biting events as
victim + 1), log(number of aggressions as attacker + 1) and log(number
of aggressions as victim + 1). Unless otherwise stated, all behavioural
results are presented as the log values. NS
Wool damage score data were analysed by split plot ANOVA, with LFF
the main plot including the effects of fibre content, feeding frequency
and their interaction, and the subplot including the effects of time
and its interaction with fibre content, feeding frequency and fibre
content × feeding frequency. The average daily gain for the duration of
the study was calculated for each animal, and analysed by ANOVA for a
2 × 2 factorial plan. Within fibre treatments, correlation analyses were S
performed between average daily gain and mean wool damage score for LFF
each animal.

Results
Wool biting was virtually absent at the start of the experiment,
but increased with time through to Week 15, with more
sheep undertaking the behaviour (Fig. 1). Figure 2 presents NS
HFF

10
No. of wool biters

6 S
HFF
4 NS, HFF
NS, LFF
S, HFF
2
S, LFF 30 20 10 0 10 20 30

0 Mean no. of events/hour

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Week Fig. 2. Mean number of wool biting events per hour for sheep (n = 40)
either fed pellets only at low feeding frequency (NS, LFF), supplemented
Fig. 1. Cumulative number of sheep observed wool biting within each with straw and fed pellets at low feeding frequency (S, LFF), fed pellets
treatment during the 15-week study. The sheep were either fed pellets only at a higher feeding frequency (NS, HFF), or supplemented with
only at low feeding frequency (NS, LFF), supplemented with straw straw and fed pellets at a higher feeding frequency (S, HFF). Open
and fed pellets at low feeding frequency (S, LFF), fed pellets only at a bars indicate individual sheep and solid bars represent the treatment
higher feeding frequency (NS, HFF), or supplemented with straw and means. Data are untransformed values and represent the mean over the
fed pellets at a higher feeding frequency (S, HFF). 15 weeks.
780 Australian Journal of Experimental Agriculture S. Vasseur et al.

** *** **
0.7
(a) *** ***
3.0
0.6 (a)

2.5
0.5 Pooled s.e.

2.0
Pooled s.e.
0.4
NS
0.3 S
1.5

0.2
1.0
Log(wool biting events/h)

0.1
0.5 NS

Wool damage score

0 S
5 10 15
0
5 10 15
0.7 (b)
* * *** ** 3.0 (b)
0.6

0.5 Pooled s.e. 2.5

0.4 2.0 Pooled s.e.

0.3
1.5
0.2
LFF 1.0
0.1
HFF
LFF
0.5
HFF
0
5 10 15
Week 0
5 10 15
Fig. 3. Wool biting events per sheep during the 15-week study period
Week
for the main effects of (a) fibre provision (NS, low fibre; S, fibre
supplement) and (b) feeding frequency (LFF, low feeding frequency; Fig. 5. Wool damage score during the 15-week study period for the
HFF, high feeding frequency). *P<0.05; **P<0.01; ***P<0.001. main effects of (a) fibre provision (NS, low fibre; S, fibre supplement)
and (b) feeding frequency (LFF, low feeding frequency; HFF, high
feeding frequency). **P<0.01; ***P<0.001.

consistent throughout the study period, although the effect

0.7 of time was significant (P = 0.002). There were no effects
of fibre provision, feeding frequency, or their interaction
Log(aggressive events/h)

0.6 (P>0.05). There was an effect of fibre provision × time

of day, whereby sheep that received fibre displayed less
0.5
aggression during the afternoon [0.22 and 0.19 log(events/h)
for NS and S, respectively, s.e. = 0.029; P = 0.013], but more
0.4
aggression during the morning [0.16 and 0.22 log(events/h)
0.3 for NS and S, respectively].
The main effects of treatment on wool damage are
0.2 presented in Figure 5. The wool damage score increased
(P<0.001) with time during the experiment. There was
0.1 an effect of fibre provision (P<0.001) and a fibre
0 5 10 15
provision × time interaction (P<0.001). Sheep on the S
Week
treatment displayed a slower increase in wool damage than
Fig. 4. Aggressive events per sheep during the 15-week study period. NS sheep during the course of the study. There were no effects
Wool biting in housed sheep Australian Journal of Experimental Agriculture 781

(P>0.05) of feeding frequency, feeding frequency × time, The effect of feeding frequency on wool biting was less
or fibre provision × feeding frequency on the wool obvious. There were effects of feeding frequency on wool
damage score. biting behaviour at some time points, but this was not
The average daily gain was 70.2 g for S sheep and 42.4 g consistent and did not result in differences in wool damage
for NS sheep (pooled s.e. = 7.72; P = 0.015). There was no scores. Contrary to our hypothesis, the effects of feeding
difference in average daily gain between LFF and HFF sheep frequency, where present, suggested that higher feeding
(54.8 and 57.8 g, respectively; pooled s.e. = 7.72; P = 0.78). frequency was associated with more wool biting behaviour.
The fibre × feeding frequency interaction was not significant This finding may result from animals being able to consume
(P = 0.91). Within fibre treatments, there were no significant all their allocated feed at once when it is presented in smaller
correlations between average daily gain and mean wool portions, providing them with less oral stimulation than when
damage score for either S sheep (r = 0.02; P = 0.93) or NS they are presented with feed in larger quantities, and return to
sheep (r = –0.16; P = 0.51). it several times during the day. However, given that any effects
of feeding frequency on wool biting were not consistently
Discussion present, it is not possible to draw robust conclusions.
The main findings of this study were that wool biting From the results of this study, the 2 behaviours of wool
developed and increased in sheep from the fifth week of biting and aggression are different and the motivations
housing, and that the provision of fibre significantly delayed resulting in their development are not the same. Incidents
and reduced the occurrence of wool biting. Furthermore, of aggressive behaviour were present throughout the study at
the provision of increased dietary fibre provided protection a consistent level, whereas wool biting took time to develop
against wool damage. and then increased. Furthermore, wool biting was not the
Although previous theories on wool biting have included sole preserve of 1 or 2 dominant animals in a pen, with
nutritional deficiencies as a possible cause (Sambraus 1985), all but 2 sheep acting as both initiators and victims of
the results of the present study indicate that the wool wool biting.
biting was more related to either a need for fibre and oral Rather than a stereotypy, which is defined as a behaviour
stimulus, or a need for activity that was satisfied by oral that is repetitive, invariant and has no obvious function
stimulus. The diets were designed to be nutritionally suitable (Odberg 1978), wool biting fits more closely with the concept
for growing sheep, and there was less than 5% difference of a redirected behaviour. The results of this study would
between the two dietary treatments in the major components support the argument that wool biting is at least partially
of energy and protein. Furthermore, all treatment groups initiated by the absence of a natural substrate for grazing and
gained weight during the study. A study by Yurtman et al. the non-satisfaction of the need for stimulation of sheep’s oral
(2002) showed that increasing the daily protein intake in organs by eating and ruminating.
lambs under energy restricted conditions may have additive With regard to its efficacy in preventing wool biting, we
effects on the development of stereotypic oral behaviours, did not test the effect of the type or source of fibre, but
such as licking and chewing inanimate objects. In the present our previous experience with housed sheep suggests that
study, the wool biting behaviour developed under conditions generally coarser fibre was better. For instance, when chaff
of adequate energy provision, although we did not examine had been fed as a supplement in the animal house before
the effect of differences in protein content. In pigs, the this experiment, wool biting still occurred. We also would
provision of straw was shown to reduce the incidence of recommend that fibre be given in a way which stimulates
anomalous oral behaviours by sows on otherwise isoenergetic the need for sheep to use their oral organs and to browse.
diets (Whittaker et al. 1998). In the present study, the large Increasing the work sheep have to undertake to extract the
difference in daily gain between the sheep on the NS and straw may be a good way of achieving this, and requires
S treatments is presumably due to the nuisance and stress the sheep to be browsing for longer without increasing the
caused by wool biting. Given that most sheep were both amount of straw provided. This may also help to counteract
aggressors and victims in wool biting, and that there was any contribution of boredom to wool biting.
no correlation between average daily gain and wool damage In conclusion, the provision of fibre in the form of straw
score within fibre treatments, it is likely that it was the within 5 by 5 cm metal mesh hay racks had a beneficial
performance and receiving of wool biting that was disruptive effect in delaying and reducing wool biting in housed
to the animals, rather than any physical harm caused. concentrate-fed sheep. Additional research is required to
The increase in wool biting during the course of the study identify whether the contribution of the fibre to altering
was not only due to a fixed number of sheep increasing their the time budgets of the sheep was part of its beneficial
habit, but also to new sheep undertaking the behaviour. This effect, through reducing time available for redirection of oral
suggests that either the sheep may learn from each other by behaviour to wool biting. Similarly, it would be useful to
visual observation, or that there is individual variation in the determine whether the provision of fibre can reduce wool
critical point from which the wool biting behaviour starts. biting once it has commenced.
782 Australian Journal of Experimental Agriculture S. Vasseur et al.

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