AIMS INTERNATIONAL SENIOR SECONDARY SCHOOL.GONDA BIOLOGY INVESTIGATORY PROJECT SESSION :2023-2024 TOPIC - DNA REPLICATION Submitted By :Pratyush Kumar Soni NAME :Pratyush Kumar Soni CLASS 2123 (PCB) ROLLNO : 26 ,Certificate 3 Acknowledgement 4 Objective 5 Introduction 6 DNA Replication Steps and Process Conclusion Summary BibliographyThis is to certify that this “Biology Investigatory Project” on the Topic “DNA REPLICATION” has been successfully completed by Pratyush Kumar Soni of class XII-A under the guidance of Mr.__ ____in partial fulfillment of the curriculum of Central Board of Secondary Education (CBSE) Leading to the award of annual examination of the year 2022-23. Teacher's Signature External Examiner’s Signature Principal’s SignatureT have taken efforts in this project. However, it would not have been possible without the kind support and help of many individuals. I would like to thank my principal Mr.Vinot Bhatt and school for providing me with facilities required to do my project. I am _ highly indebted to my _ Biology teacher , or his invaluable, guidance which has sustained my efforts in all the stages of this project work. I would also like to thank my parents for their continuous support and encouragement. My thanks and appreciations also go to my fellow classmates and the laboratory assistant in developing the project and to the people who have willingly helped me out with their abilities.The goal of replication of is to Produce a Second and identical Double stand. Because each of the two Stands in the DNA molecule Serves as a Template for a New DNA stand, the First step DNA Replication is to separate DNA.DAN replication, or the urging of a cell’s DAN a no simple teak; there are about 3 billion have pairs of DAN in are genome all of which must be accurately copied when any one of four trillions of cells deviates The basic mechanism of DNA replication is similar across organisms. In this article, we ’we II fours on DNA replication E. unit, but the mechanism of replication are similar in humans and other eukaryotes. Let’s take a look at the proteins and enzymes that carry out replication, seeing how they work together to ensure accurate and complete replication of DNA. Page 6There were, three, basic mescals for DNA replication that had been proposed by the — MABEDEDNamEBLaCaTION scientific community after the discovery of DNA's structure. a) Semiconservative mode! b) Conservative mode! ¢) Dispersive model < < 1 Ss Ss > eo fen ene S S S ~3§ $5255 $5.55 6b oiication §. SS=SS SS= ES SESS © Sami —conservative re replication In this model , the two strands of DAN Unwind from each acts as a template for synthesis of new, complementary strand.. this rewets in tow DAN molecules with me original strand and me new strand . © Conservative replication In this model DAN replication results in ore molecule that consist of both original DNA strands (indent to the original DAN Molecule ) and another more cue that consist of two new strands (with erectly the same sequences as the original molecule). ® Dispersive replication In the dispersive model, DNA replication results in tow DNA molecules that are mixtures, or’ hybrids”, of parental and daughter DNA . In this model , each individual strand is a patchwork of original and new DNA. Page 7Why Replicate DNA? DNA is the genetic material that defines every cell. Before a cell duplicates and is divided into new daughter cells through _ either mitosis or meiosis, biomolecules and organelles must be copied to be distributed among the cells. DNA, found within the nucleus, must be replicated in order to ensure that each new cell receives the correct number of chromosomes. The process of DNA duplication is called DNA replication. Replication follows several steps that involve multiple proteins called replication enzymes and RNA. In eukaryotic cells, such as animal cells and plant cells, DNA replication occurs in the S phase of interphase during the cell cycle. The process of DNA replication is vital for cell growth, repair, and reproduction in organisms. Key Takeaways + Deoxyribonucleic acid, commonly known as DNA, is a nucleic acid that has three main components: a deoxyribose sugar, a phosphate, and a nitrogenous base. + Since DNA contains the genetic material for an organism, it is important that it be copied when a cell divides into daughter cells. The process that copies DNA is called replication. + Replication involves the production of identical helices of DNA from one double- stranded molecule of DNA. + Enzymes are vital to DNA replication since they catalyze very important steps in the process. + The overall DNA replication process is extremely important for both cell growth and reproduction in organisms. It is also vital in the cell repair process. Page 8DNA Structure DNA or deoxyribonucleic acid is a type of molecule known as a nucleic acid. It consists of a 5-carbon deoxyribose sugar, a phosphate, and a nitrogenous base. Double-stranded DNA consists of two spiral nucleic acid chains that are twisted into a double helix shape. This twisting allows DNA to be more compact. In order to fit within the nucleus, DNA is packed into tightly coiled structures called chromatin. Chromatin condenses __ to form chromosomes during cell division. Prior to DNA replication, the chromatin loosens giving cell replication machinery access to the DNA strands. Page 9Step 1: Replication Fork Formation Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands. DNA has four bases called adenine (A), thymine (T), cytosine (C) and guanine (G) that form pairs between the two strands. Adenine only pairs with thymine and cytosine only binds with guanine. In order to unwind DNA, these interactions between base pairs must be broken. This is performed by an enzyme known as DNA helicase. DNA helicase disrupts the hydrogen bonding between base pairs to separate the strands into a Y shape known as the replication fork. This area will be the template for replication to begin. DNA is directional in both strands, signified by a 5’ and 3" end. This notation signifies which side group is attached the DNA backbone. The 5" end has a phosphate (P) group attached, while the 3" end has a hydroxyl (OH) group attached. This directionality is important for replication as it only progresses in the 5' to 3' direction. However, the replication fork is bi- directional: one strand is oriented in the 3' to 5' direction (leading strand) while the other is oriented 5' to 3' (lagging strand). The two sides are therefore replicated with two different processes to accommodate the directional difference. REPLICATION BEGINS Step 2: Primer Binding The leading strand is the simplest to replicate. Once the DNA strands have been separated, a short piece of RNA called a primer binds to the 3" end of the strand. The primer always binds as the starting point for replication. Primers are generated by the enzyme DNA primase.Step 3: Elongation Enzymes known as DNA polymerases arc responsible creating the new strand by a process called elongation. There are five different known types of DNA polymerases in bacteria and human cells. In bacteria such as E. coli, polymerase II is the main replication enzyme, while polymerase I, I, IV and V are responsible for error checking and repair. DNA Page 11polymerase III binds to the strand at the site of the primer and begins adding new base pairs complementary to the strand during replication. In eukaryotic cells, polymerases alpha, delta, and epsilon are the primary polymerases involved in DNA replication. Because replication proceeds in the 5' to 3' direction on the leading strand, the newly formed strand is continuous. The lagging strand begins replication by binding with multiple primers. Each primer is only several bases apart. DNA polymerase then adds pieces of DNA, called Okazaki fragments, to the strand between primers. This process of replication is discontinuous as the newly created fragments are disjointed. Step 4: Termination Once both the continuous and discontinuous strands are formed, an enzyme called exonuclease removes all RNA primers from the original strands. These primers are then replaced with appropriate bases. Another exonuclease “proofreads” the newly formed DNA to check, remove and replace any errors. Another enzyme called DNA ligase joins Okazaki fragments together forming a single unified strand. The ends of the linear DNA present a problem as DNA polymerase can only add nucleotides in the 5' to 3’ direction. The ends of the parent strands consist of repeated DNA sequences called telomeres. Telomeres act as protective caps at the end of chromosomes to prevent nearby chromosomes from fusing. A special type of DNA polymerase enzyme called telomerase catalyzes the synthesis of telomere sequences at the ends of the DNA. Once completed, the parent strand and its complementary DNA strand coils into the familiar double helix shape. In the end, replication produces two DNA molecules, each with one strand from the parent molecule and one new strand. rage seReplication Enzymes DNA replication would not occur without enzymes that catalyze various steps in the process Enzymes that participate in the eukaryotic DNA replication process include Page 13,+ DNA helicase - unwinds and separates double stranded DNA as it moves along the DNA. It forms the replication fork by breaking hydrogen bonds between nucleotide pairs in DNA + DNA primase - a type of RNA polymerase that generates RNA primers. Primers are short RNA molecules that act as templates for the starting point of DNA replication. + DNA polymerases - synthesize new DNA molecules by adding nucleotides to leading and lagging DNA strands. + Topoisomerase or DNA Gyrase - unwinds and rewinds DNA strands to prevent the DNA from becoming tangled or supercoiled. + Exonucleases - group of enzymes that remove nucleotide bases from the end of a DNA chain. + DNA ligase - joins DNA fragments together by forming phosphodiester bonds between nucleotides. DNA Replication Summary DNA replication is the production of identical DNA helices from a single double- stranded DNA molecule. Each molecule consists of a strand from the original molecule and a newly formed strand. Prior to replication, the DNA uncoils and strands separate. A replication fork is formed which serves as a template for replication. Primers bind to the DNA and DNA polymerases add new nucleotide sequences in the 5’ to 3’ direction. Page 14CONCLUSIONS The secondary structure of DNA is important in many events in cellular life. Replication, transcription and regulation of expression of many genes depends on local differences or changes in DNA structure. Recombination which leads to rearrangement of genes takes advantage of the ability to form an unusual structure called a Holiday's structure. Also different kinds of mutations occur as a result of specific DNA structure.BIBLOGRAPHY > www.wikipediaorg +» NCERT biology textbook for class 12" + Exploring biology by Arihant Publications > http://www.youtube.com/c/ALLINVESTIGATORYPROJECTS +> https://t.me/allinvestigatoryprojects --THANK YOUALLINVESTIGATORYPROLECTS THANK