Mortality of Golden Apple Snail

Off-season Mortality of Golden Apple Snail, Pomacea canaliculata (Lamarck) and its Management Implications Abstract
RANEE ENRIQUEZ JOSHI, University Science High School, Central Luzon State University, Science City of Muoz, Nueva Ecija, Philippines, April 2005, OFF-SEASON MORTALITY OF GOLDEN APPLE SNAIL, POMACEA CANALICULATA (LAMARCK) AND ITS MANAGEMENT IMPLICATIONS. Adviser: Dr. Rolando D. Dollete The study was conducted to determine whether GAS size and GAS sex has any relation with the off-season mortality of GAS and to provide management implications in accordance to the results obtained. The experiments for GAS size and sex were conducted in netbags and plastic trays with soil. Results showed that generally, GAS have low mortality rates but increased with their size. With GAS sex, no consistent mortality trends were observed. Mortality rates in plastic trays proved higher than those in netbags. It could be due to lack of aeration and low air exchange. Proper management implications have been derived by giving a rice cropping calendar which would help sustain yields and reduce GAS damage.
Introduction
The golden apple snail (GAS), Pomacea canaliculata (Lamarck), originating from South America, has increased its invasiveness and damage to rice plants in Asia and North America where it was introduced. It is known by an array of common names such as: golden miracle snail, golden snail, jumbo snail, Argentinian apple snail, bisocol and golden kuhol. The term golden refers not to their color, but to the amount of money snail wranglers could make raising them. However, GAS is its frequently used common name. It is listed as one of the Worlds Worst
Invasive Alien Species (IAS) as its invasion ability is based on unique morphological and biological characteristics that support their capacity to survive in adverse environmental conditions and still reproduce fast.
GAS has become a major pest of rice in all the rice-growing countries where it was either intentionally or accidentally introduced (Joshi et al., 2003a). The most recent serious invasions are reported from Republic of Dominica, Papua New Guinea and South Korea. GAS continues to be a problem in Japan, Thailand, Vietnam, Cambodia and Malaysia. In Asia, distribution continues to expand westward. Large ricegrowing regions of India, Bangladesh, China and Australia are the probable next targets of GAS invasions. From the information gathered in the given countries, it could be generalized that: (1) four years after its introduction in a country, it attains the pest status; (2) local establishment is a key feature of invasion; (3) establishment is stochastic; (4) once established, it will typically persist; and (5) invasion-resistance increases during assembly.
In the Philippines, the government promoted GAS production in 1982 and 1984, as a national livelihood program to increase the protein intake of low-income Filipino rice farmers and as an additional source of their income (Ang, 1984; Adalla and Rejesus, 1989). Due to improper
rearing, GAS escaped into the rice paddies and infested 425.862 ha (11% of the total rice cropped area) in the Philippines (Rondon and Callo, 1989). The losses to Philippine rice crops from GAS in 1980 are tuned to US$ 1 billion in 1980s. Annual global agricultural economic losses from GAS range from 55-248 billion/year. By 1990, GAS infected area increased to more than 600,000 ha. Surveys conducted in 1991, showed that the infestation increased to 900,000 ha (Bayer, 1992). At present, it is a major biotic constraint in all regions and rice ecosystems of the Philippines (Alviola et al., 2000), including the Ifugao Rice Terraces (Dancel and Joshi, 2000; Joshi et al., 2001). In addition, to GAS being ranked as a pest of national importance, it is blamed for the decline of edible native apple snail, Pila conica (Gray) and the jojo or yoyo (Mirgurnus
anguillicaudatus) in the Ifugao Rice Terraces. This is probably because of the competition for common habitat and resources (Halwart, 1994).
GAS being a highly voracious nocturnal herbivore destroys newly transplanted rice (Saxena et al., 1987). GAS damage is characterized by missing hills and floating leaf fragments in the rice field (Joshi et al., 2002). GAS cuts the base of young seedlings with its layered tooth (radula) and eats the succulent, tender rice leaves. The extent of damage to the rice crop depends on snail size, snail density, and growth stage of the rice plant. A density of three GAS per square meter causes significant yield
loss, with much greater damage to direct -seeded rice and young seedlings transplanted at 18-21 days (Litsinger and Estao 1993). GAS with 40-mm is generally the most destructive size, irrespective of the rice establishment method. It causes 100% destruction of the rice seedlings in the germinating stage and at least 20% in transplanted seedlings. At 30 days after transplanting, medium-sized snail (2-3 cm shell height) at a density of one and eight snails per square meter reduced the number of rice tillers by 19% and 98%, respectively (Basilio, 1991). GAS of 10-mm size was capable of causing damage to direct -seeded rice even after 1 day. However, 5-mm sized GAS did not damage rice seedlings; instead, they fed on algae and other organic matter at the field water surface. GAS adults also feed on azolla, morning glory, sweet potato, taro and other aquatic plants. Adults measuring 22-26 mm consume up to 15 grams of azolla in 12-24 hr (Saxena et al., 1987). GAS damage is severe in lower portions of the fields where water stagnates.
Hence, because of the undeniable significance and hazard of GAS to rice plants, this study investigated the natural mortality rates of GAS, after rice harvesting but before rice planting (off-season). This baseline information is necessary to tackle GAS and facilitate its size prediction during rice growing periods and developing environment -friendly and socially acceptable management options.
The study determined the mortality rates of various GAS sizes and sexes during off-season. Off-season is the time interval between the last harvesting and the succeeding planting season. During these periods, GAS aestivates in soil or crop residues in rice fields. GAS stores nutrients and minerals prior to aestivation. During aestivation, GAS closes its lid with its mucous and buries inside the soil and remains immobile until the rains have arrived. A lot of GAS probably die in the process of hibernation. Hence, this study quantified the mortality rates and patterns on various GAS sizes and sexes. From this information, the study also advised the proper management implications.
The
knowledge
gained
in
this
study
is
essential
for
GAS
management in rice farming. It will guide the farmers and extension workers as to which GAS sizes they would have to deal with during rice planting time (either in direct -seeded or transplanted rice systems). In addition, this study established information on managing dominant GAS sizes and sexes for paddy weeding in lowland transplanted irrigated rice farming.
Statement of the Problem The following were the problems of the study: 1. What are the mortality rates of GAS during off-season in relation with their size? 2. What are the mortality rates of GAS during off-season in relation with their sex? 3. What are the management implications in relation with the various GAS sizes and sexes?
Objectives of the Study The following were the objectives of the study: 1. To determine the mortality rates of GAS during off-season in relation with their size. 2. To determine the mortality rates of GAS during off-season in relation with their sex. 3. To determine the management implications in relation with the various GAS sizes and sexes.
Hypothesis of the Study The hypothesis of the study was that the mortality rates were based on the variables; sex and size cannot contribute to any management implications.
Significance of the Study Research on the mortality of GAS suggests a more comprehensive approach. Since there are very limited studies that employ the off-season mortality of GA S, this exploratory study contributed information to the growing body of the knowledge relevant to the rice researchers and farmers. Moreover, it contributed to proper GAS management
implications and development of research methodology and procedures useful in the field problems.
Until September 2004, the different agencies of the Department of Agriculture, Philippines have not conducted a research on this aspect . The study filled the knowledge and information gaps relative to the offseason GAS mortality, particularly to the rice farm management extension staff and rice farmers. It would also give proper GAS management options to reduce the use of synthetic molluscicides.
Scope and Limitations of the Study The study is confined itself in observing the off-season mortality rate of GAS. It only considered t wo variables, namely: size and sex. The study was completed in two months. The samples were collected in Maligaya, Science City of Muoz, Nueva Ecija. The management implications were based on previously studied management options.
The only major limitation that may have affected the validity and accuracy of the study is the condition in which the samples were retained not in their natural habitat/environment.
Operational Definition of Terms 1. Mortality It refers to the population decrease factor or death rate of GAS. 2. Size It is the parameter was used to classify the samples. There were 8 definite sizes used ranging from 0 mm to 40 mm namely: 5 mm, 10 mm, 15 mm, 20 mm, 25 mm, 30 mm, 35 mm and 40 mm. Each range had a less than 1mm span. 3. Sex It refers to the gender of each snail, male or female. 4. Management Implications These are the different GAS management options suggested based on the gathered data from the study of the mortality rate of GAS.
Review of Related Literature
This chapter includes related readings, researches and references to this study which would help understand the topic of the research paper.
GAS Mortality Snails larger than 6 mm exhibit higher cold tolerance than small snails as observed in Japan. Temperature is a practical and effective parameter in estimating mortality of field snails (Syobu et al., 2001). The apple snails are proven susceptible to low temperatures. They die within 35 days at 0C, 3 days at 3C and 1 day at 6C (Oya et al., 1987). Unlike in Japan, the Philippines is a tropical country which makes the environment more favorable to GAS. The information about the mortality rate of GAS in the Philippines during the off-season is not thoroughly established, and this is what this study sought to provide.
Agrochemicals for GAS Management Different agencies have developed and recommended integrated management methods for GAS, but Filipino farmers use commercially available synthetic molluscicides as their first -line of defense, without considering the toxic hazards to themselves and non-target organisms.
Result surveys in the Philippines support this, the survey showed that 75100% of the rice farmers consider GAS to be the most serious pest problem in rice, with more than 40% applying pesticides (Revilla et al., 1001; Ketelaar, 1993). In 1988, the synthetic molluscicide expenditure was estimated at US$ 2.4 million (Halwart, 1994). In 1993, farmers spent about US$ 9 ha-1 for pest management (Medrano et al., 1993). This figure swelled to US$ 23 ha-1 despite the proper use of registered commercial molluscicides. In other parts of Asia, like Taiwan, they have spent more than US$ 1 million per year on molluscicides for treatment of 100,000 ha of rice fields (Cheng, 1989).
The chemical approach is unsuitable for the resource-poor rice farmers. Aside from the impracticality of using synthetic molluscicides, the use of organo-tin compounds further caused several human health risks, such as skin peeling in fingers and toes, head aches, skin disorders, blindness and even casualties (Anderson, 1993). These compounds caused high toxicities on fish especially in rice-fish farming systems (Hausen, 1993). Eventually, organo-tin compounds were banned. In
addition, the use of pesticides for GAS control is difficult as GAS bury themselves in the soil to avoid exposure from the pesticide sprays (Dela Cruz et al., 2000; Dela Cruz and Joshi, 2001a).
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Plant Molluscicides Neem, Azadirachta indica, has come under close scientific scrutiny as a source of unique natural products for integrated pest management (Jacobson, 1989; Saxena, 1898; Schmutter, 1990, 2002a; Ascher, 1993). Schmutter (2002b) has reviewed the effectiveness of various neem materials against mollusks. Muley (1978) has stated that 100% mortality of the snail Melania scabra occurred in 20 minutes when treated with 0.5% tap water extract of dried neem seed powder. Neem seed extract was likewise toxic to Biomphalaria glabrata (Jacobson, 1989). Dried leaves were treated with Lymnea luteola and Gyraulus convexiusculus that caused them to die within 24 hours (Bali and Pati, 1985). Its leaf, bark and fruit were also tested on other mollusks and had successful results. Eventually, Maini and Rejesus (1993) tested aqueous neem leaf and seed extracts, neem oil and Bioblitz against GAS. Leaf and seed extracts were the most toxic causing 100% snail mortality at 100 ppm after 48 hours. Effects of neem treatments on the ecology of the snails are still to be investigated. Aqueous Neem Seed Kernel Extract (NSKE) was tested against non-operculated and operculated freshwater snails (Mossalam et al., 1994). Treatment with 2.5% NSKE killed four non-operculated snail species after 24 hours while the operculated species died in 24 hours when under 5% NSKE.
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Vulgarone-B is another plant molluscicide isolated from crude oil by silica gel column chromatography of Artemisia douglasiana Besser aerial parts. The study of Joshi et al. (unpublished) has stated that Vulgarone-B has activity as molluscicide is comparable to that of metaldehyde (commercial molluscicide) in a laboratory bioassay indicating 100% mortality of GAS in 24 hours.
Naturally-Occurring Control Agents Red ants and long-horned grasshopper predate on GAS egg masses, while ducks and rats consume their shells and meat
(www.knowledgebank.irri.org/tropRice). Herding ducks into the rice fields during final land preparation or after crop establishment is therefore advised. Duck herding together with feed supplementation during their confinement can enhance egg production from 60 to 70% egg (Tacio, 1987). In rice-fish-duck-azolla farming system, duck-laying percentage was at an average of 60% (Cagauan, 1999).
Cultural and Physical Management The shift from transplanted rice to direct -seeded rice culture even cause bigger GAS nuisance in the later crop establishment method. This is labor-intensive because missing hills should be replanted. Good field
12
leveling and shallow water management practices are key options to reduce the GAS damage in lowland irrigated transplanted rice systems, but this practice is extremely difficult to adopt in direct -seeded and upland rice ecosystems and in flood-prone areas.
Installing metal screens at water inlets has been recommended to minimize the entry of large-sized GAS into the rice fields and to facilitate hand-collection, but small GAS can still enter undetected. Hand-picking GAS and crushing GAS egg masses by using hand-operated smashing devices are highly labor-intensive practices and unfeasible in large paddy fields. Mechanical control of GAS by rotary cultivator is efficient, as it can decrease their density drastically (Takahashi et al., 2002a). In submerged direct sown field, the GAS damaged 48.1 % of its area. However in the field where rotary cultivation was practiced it was only 2.3% (Takahashi et al., 2002b). Mochida (1988) also reported that the use of roto-tiller during land preparation is beneficial as it resulted to about 27% GAS mortality as compared to the unploughed fields.
Integrated Management Options Despite GAS being classified as invasive, it can still be managed. GAS can be utilized as an animal feed and human food. It is now considered as a replacement for meat or fish meal in animal diets. The
13
protein content (62.5%) is comparable to the CP value of Peruvian Fish meal (61.2%) but a little lower than the meat meal (66%) (Gerpacio and Castillo, 1979). Uncooked GAS meal in swine diets can be used up to 15% (Catalma et al., 1991a) and up to 10% in the diet of native chicks (Catalma et al., 1991b).
Nile Tilapia in aquaria when fed with GAS meat meal at 75-100% of the diet mixed with rice bran was beneficial and cost -effective (Cagauan and Doria, 1989). Similarly, in cage culture of Nile tilapia, snail-meal based diet was superior over the fish fed with fishmeal-based diet (Reazo, 1988). In the freshwater praw n larvae (Macrobrachium rosenbergii), 60% GAS meal in dried form mixed with rice bran, shrimp meal and fish meal gave good growth results (Lansangan et al., 2002).
As human food, GAS is cooked with coconut milk or made into kropeck. One major hindrance in the feasibility of GAS recipes is their short shelf life. Moreover, the latest GAS recipe is the chicharon kuhol (cracker) which is devoid of water, odorless and has a longer shelf life (Dela Cruz and Joshi, 2001b).
GAS can also be used as bioweeder in transplanted rice systems. Paddy weeding is practiced by some organic and inorganic farmers in
14
Japan, Philippines and South Korea (Okuma et al., 1994; Wada et al., 2002, Yusa et al., 2003; Joshi et al., 2003a). GAS is employed to feed on aquatic weeds. Utilizing GAS for weeding is less laborious, more economical and care-free when compared with ducks, carp or tadpole shrimps (Yusa et al., 2003). In areas, not invaded by GAS, paddy weeding should be strictly prohibited.
15
Methodology
This chapter contains the different materials and methods used in the different experiments and explain how the management implications were derived.
GAS Mortalities The study was conducted in Maligaya, Science City of Muoz, Nueva Ecija. With the help and support of Department of AgriculturePhilippine Rice Research Institute (DA -PhilRice), the proponent had access to the CPD headhouse No.6 and other equipment. The experiments utilized various sizes and sexes of Golden Apple Snail, Pomacea canaliculata (Lamarck) from October 30, 2004 to December 31, 2004.
Experiment 1. GAS Mortality of Different GAS Sizes in Netbag Treatment GAS were collected from water-logged rice fields, irrigation canals and fish ponds. They were sorted for standard sizes using a Mitutoyo digital caliper (range of 1mm) (Plate 1). The GAS sizes were 10, 15, 20, 25, 30, 35 and 40-mm. The GAS were then colored white with an OFFICE correction fluid and numbered individually for proper identification using a N60 Pentel Pen permanent marker (Plate 2 & 3). GAS were weighed individually in a Mettler AE 240 digital balance (Plate 4).
16
Plate 1. Sorting of GAS sizes and sex.
Plate 2. Drying the marked shell of the GAS.
17
Plate 3. Assigning numbers to GAS.
Plate 4. Weighing marked GAS individually in the Metler AE 240 digital balance.
18
One hundred individuals per size were put in each netbag with the dimensions 24 x 12 (Plate 5). The netbags were labeled to avoid misidentification. Every two weeks, ten GAS were taken out from each netbag. They were weighed again using a Mettler AE 240 digital balance and were submerged in water for 2h in order to determine if they were alive or dead. On the eighth week, the remaining 70 GAS were weighed individually and submerged in water together to make the final assessment of mortality.
Plate 5. GAS sizes and sexes in netbags.
Experiment 2. GAS Mortality of Different GAS Sizes in Plastic Tray with Soil Treatment The same procedure on GAS was followed except that in Experiment 2 where GAS were placed in 6 x 6 plastic trays in which one
19
inch of paddy field soil was placed (Plate 6). After the soil has been levelled, 300 ml water was added to each tray. Ten plastic trays were used for each GAS size. In each plastic tray, five snails were buried into the soil. Following this, dried soil was added until the plastic tray was filled (Plate 7). The trays were then covered with a plastic lid having 64 holes for proper ventilation. Gypsum blocks were put in one plastic tray of each size, to get the soil moisture readings. Moisture readings were taken every two weeks. The mortality of the GAS were checked on the 56 th day after treatment. The GAS were not weighed as it was impossible to remove the soil sticking on the shell.
Plate 6. The plastic trays used for the experiment were filled with one-inch paddy field soil.
20
Plate 7. GAS in plastic tray with water and paddy soil.
Experiment 3. GAS Mortality of Different GAS Sexes in Netbag Treatment The same procedure was done to the GAS as in Experiment 1. The GAS were further classified according to their sex. GAS of 10 mm and 15 mm were not included in this experiment as their sex could not be determined. Twenty-five GAS per sex were put for each netbag. GAS were individually weighted after 56 days and then submerged in water to confirm if they were dead or alive.
Experiment 4. GAS Mortality of Different GAS Sexes in Plastic Tray with Soil Treatment The same method in Experiment 3 was followed except that they were put in 6 x 6 plastic trays with paddy field soil (Plate 8). Twenty- five
21
GAS per sex were used, with 5 GAS per tray. The mortality was checked on the 56th day as done in the earlier experiments (Plate 9).
Management Implications From the results of the four experiments, management implications were arrived at and presented as the second phase of this study. These management implications are recommendations to manage GAS.
Plate 8. The set -up of the plastic tray experiments.
22
Plate 9. Observations for GAS mortality.
23
Results and Discussion
This chapter presents the results based on the experiments conducted. This also includes discussions on suggested management implications.
GAS Mortalities In all four experiments, GAS mortalities of all sizes and sexes showed that small-sized GAS took longer time to become active. This is probably because in smallsized GAS the operculum was tightly sealed with the mucus (saliva) (Plate 10). No relationships were observed for either sex.
Plate 10. GAS observations on mortality after one hour.
24
Experiment 1. GAS Mortality of Different GAS Sizes in Netbag Treatment
GAS Mortality (%)
60 50 40 30 20 10 0 10 15 20 25 30 35 40 GAS Size (mm)
2nd week 4th week 6th week 8th week
Figure 1. GAS mortality (%) at 2nd, 4th , 6th and 8th week after storage in netbags, October 30-December 31, 2004 (N=10 for 2nd, 4th and 6th week and N=70 for 8 th week). GAS mortality (%) increased as the size of the GAS increased (Figure 1). During the conduct of this experiment, it was observed that the largesized GAS were more susceptible to the larvae of parasitic flies (Plate 11). This would have caused greater loss of body fluids and thus induced death. The 10-mm GAS mortality remained stagnant throughout the period of observation.
The distinct patterns in mortality were not observed in the 15-mm and 25-mm GAS which could not be explained fully. This could be possibly because there were some dead GAS individuals in the netbags that would have triggered death of other remaining GAS.
25
Plate 11. GAS with the parasitic fly larva. (Note: white larva protruding out of the GAS operculum)
Table 1. GAS weight loss (%) in the netbags on the final day of observation. GAS Alive Dead Size Initial Wt. Final Wt. Weight Initial Wt. Final Wt. Weight * (mm) (g) (g) Loss (%) (g) (g) Loss (%) (X S.D.) (X S.D.) (X S.D.) (X S.D.) (X S.D.) (X S.D.) 10 0.3 0.1 0.2 0.1 29.0 8.2 0.3 0.1 0.1 0.1 53.6 18.0 15 0.7 0.2 0.5 0.2 23.9 8.1 0.6 0.2 0.3 0.1 52.7 15.1 20 1.6 0.2 1.3 0.2 20.4 3.5 1.4 0.3 0.8 0.2 40.5 14.3 25 3.0 0.3 2.5 0.4 18.2 7.7 2.7 0.5 1.2 0.4 54.7 13.6 30 5.1 0.6 4.2 0.7 18.3 6.7 4.7 0.4 2.1 0.5 55.0 13.0 35 6.7 1.0 5.4 1.2 19.8 10.2 6.2 1.0 2.8 0.9 55.3 12.6 40 9.4 1.6 7.3 1.7 22.5 8.3 8.0 1.2 3.7 1.2 53.7 14.1 * N=70 per size. Initial weights of dead and alive GAS were similar at the start of the experiment (Table 1). The standard deviation (S.D.) values were very small, suggesting variation within weights were negligible to cause experimental
26
errors. Weight loss (%) in alive GAS ranged from 18.2 to 29.0 with a maximum standard deviation of 10.2. On contrary, GAS that were dead on the eighth week of storage lost weight (40.5-55.3) with a much higher standard deviation value. This loss in weight is probably related to the loss of body fluids during the storage as it is known that GAS utilize stored foods for their metabolism during dormancy. The weight loss (%) is a reliable indicator to determine if the GAS is dead or alive, rather than deciding the status solely o the initial and final weights or by immersing GAS in n water. Hence in the future, this indicator can be used by researchers to study the long-term GAS mortality patterns. This is because even though the shell size was same (as measured by digital caliper); they differed significantly in their body weights.
Experiment 2. GAS Mortality of Different GAS Sizes in Plastic Tray with Soil Treatment GAS mortality across all sizes was clearly higher in plastic trays compared with those in netbags (Figure 2). The mortality gradient in netbag was ascending as the size of the GAS increased. However, in plastic trays the mortality patterns were erratic (inconsistent trends). This is probably because there was less air flow in plastic trays that hindered their respiration even though GAS has both gills and lungs.
27
GAS Mortality (%)
80 plastic trays 60 40 20 0 10 15 20 25 30 35 40 GAS Size (mm) netbags
Figure 2. GAS mortality (%) observed in plastic trays and netbags at the end of the 8th week of storage (N=50 for plastic trays and N=70 for netbags). Experiment 3. GAS Mortality of Different GAS Sexes in Netbag Treatment There were no significant patterns of initial weight, final weight and weight loss (%) in relation with GAS sex that could be established (Table 2). Alive GAS were similar to the results of Experiment 1 although with a much lower weight loss (range of 16.2-25.9 %) and standard deviation (9.3). The weight loss (%) for the dead GAS ranged from 49.7 to 64.7 with a maximum standard deviation of 21. This experiment verifies the findings in Experiment 1 that showed that weight loss (%) is a more reliable indicator rather than weight, to know whether the GAS is alive or dead.
28
Table 2. GAS weight loss (%) in males and females of various sizes in netbags on the last sampling date. Alive Dead GAS Initial Wt. Final Wt. Weight Initial Wt. Final Wt. Weight Loss Size Sex (g) (g) Loss (%) (g) (g) (%) * (mm) (X S.D.) (X S.D.) (X S.D.) (X S.D.) (X S.D.) (X S.D.) M 2.1 0.2 1.6 0.2 25.9 4.2 2.3 0.1 1.1 0.1 53.1 0.6 20 F 1.5 0.2 1.2 0.2 22.3 5.2 1.4 0.2 0.5 0.2 64.7 8.7 M 3.0 0.4 2.3 0.4 23.3 6.4 2.4 0.2 1.2 0.3 50.1 12.5 25 F 2.9 0.4 2.3 0.4 19.9 4.1 2.6 0.3 1.3 0.2 49.7 8.4 M 5.1 0.6 4.1 0.7 19.9 5.6 4.0 0.8 1.7 0.1 55.9 11.7 30 F 5.1 0.5 4.1 0.6 19.1 5.9 4.1 0.8 1.6 0.4 59.1 12.3 M 6.4 1.2 5.2 1.4 20.0 9.3 6.6 0.7 3.2 0.9 57.4 21.0 35 F 6.8 1.0 5.1 1.0 24.1 8.0 6.3 1.4 3.0 0.1 52.4 12.7 M 9.4 2.0 7.9 2.0 16.2 4.5 8.2 1.7 3.6 1.0 54.6 14.9 40 F 9.0 1.0 6.9 1.3 23.6 7.7 7.8 0.8 3.0 1.1 61.0 12.4
*N=25
for each sex and size.
Mortality Percentage
60 50 40 30 20 10 0 20 25 30 35 40 GAS Size (mm) Male Female
Figure 3. GAS mortality (%) at 8th week after storage in netbags, October 30-December 31, 2004 (N=25 for each sex and size).
29
The GAS mortality levels in relation to its sex were extremely variable (Figure 3). No consistent patterns were observed. It could only be observed that the mortality rate of the female GAS increased as the size increased, but suddenly decreased at 40-mm. In males, morality percentage increased except in 30-mm GAS. Therefore, there is no relationship between GAS sex and mortality percentage under netbag experiment.
Experiment 4. GAS Mortality of Different GAS Sexes in Plastic Tray with Soil Treatment
Mortality Percentage
80 60 40 20 0 20 25 30 GAS Size (mm) 35 40 Male Female
Figure 4. GAS mortality (%) at 8th week after storage in plastic trays, October 30-December 31, 2004 (N=25 for each sex and size). The mortality patterns of the males and females GAS varied. The mortality percentages of the males increased as their size increased just as it was observed in Experiment 1. However, mortality in females was
30
inconsistent. Very different from the previous experiments, the mortality in 40-mm were similar for both sexes (Figure 4).
Management Implications In rice farming, integrated crop management practices are necessary to sustain high yields. GAS being one of the major rice pest needs to be properly managed. Based on this study, the best way to increase natural mortality of GAS prior to rice farming is to increase the per cent of weight loss by enhancing the loss of body fluids. There are several options which need to be integrated with the rice cropping calendar. Briefly, they are as follows (Table 3):
The
GAS
mortalities
and
GAS
sizes
were
interdependent. Small-sized GAS (10-15 mm) had the lowest mortality in the netbags (not including in plastic trays), which means that during land preparation, exposing GAS to sunlight by deep ploughing would enhance desiccation and thus produce sizerelated mortalities. Once the GAS were exposed, they could also be predated by birds and rats. The GAS egg masses also serve as food for the long-horned grasshopper and red ants.
31
After flooding, but prior to harrowing, releasing ducks would again accelerate size-related reduction. Ducks are known to feed on GAS up to 15-mm. Table 3. Farmers activities, possible options, their effects and possible farmers acceptance. Farmers Management Possible Effects Farmers Possible Activities Implications Acceptance/Practice Land Soaking Duck Herding Itching Highly Variable Food for Ducks Manual Labor-Intensive Extremely High Collection Incomegenerating Harrowing Labor-Intensive Already Practiced Land Manual Labor-Intensive Extremely High Preparation Collection Incomegenerating Installation of Labor-Intensive Low Screens in Expensive Water Inlets Not effective Crop Pest Manual Labor-Intensive Extremely High Management Collection Incomegenerating Application of Expensive High Agrochemicals Fatal to Nonand Botanicals Target Organisms Proper Water Labor-Intensive Extremely High Management Harvesting Manual Labor-Intensive Extremely High Collection Incomegenerating After Harvest Deep Ploughing Increase Extremely High Mortality
After duck herding, the use of old newspapers to attract GAS, can make the manual picking of GAS easier and faster. Leaves of gabi, banana, papaya, trumpet flower, kangkong
32
and sweet potato could be used as attractants in areas were they are readily available. Manual collection should be done during the early morning and late afternoon, as GAS are highly active during those times. Manual collection is an attractive option for farm workers as they can use GAS for food, as well as feed for farm animals (ducks, pigs, prawns and fishes).
During harrowing, the use of rotary weeder can further enhance GAS mortality, Takahashi et. at (2002b).
In the past, use of metal screens in the water inlets and manual/mechanical collection of GAS egg masses has been suggested, but farmers found these methods labor-intensive and impractical (Joshi ). It is recommended that when the soil is harrowed, the seedbeds should be raised to avoid GAS feeding on the seedlings.
During transplanting, shallow paddy water must be maintained at 1-2 cm deep starting three days after transplanting. This is the most destructive stage of GAS; therefore water management is the key to GAS management.
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Application of agrochemicals and botanicals are not advised because they could also harm and kill non-target organisms. Aside from that they are beyond the reach of resourcepoor farmers.
By following this cropping calendar GAS mortalities could be increased and rice yields could be sustained and improved. This calendar (Table 3) was designed based from the results obtained in this study. It also aims to help rice technicians, extension workers and farmers, to have an integrated approach to GAS management that is socially-acceptable, economical, sustainable and environmental-friendly.
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Summary, Conclusions and Recommendation
Summary GAS is a major rice pest in all rice-growing countries. Its damage could range from 20-100% from the germinating to the transplanting stage. The extent of the damage can be determined by snail size, snail density and rice crop stage. Hence, it is highly significant to study the mortality rates of GAS before the planting season to be able to predict the GAS size dominant in the rice field.
The study aimed to determine if there is a relationship between GAS off-season mortality and GAS size and sex. The experiments were conducted in netbags and plastic trays with soil. GAS in netbags represent the GAS exposed while GAS in plastic trays were the GAS that aestivated. From the baseline information of the conducted experiments
management implicat ions were suggested.
Results showed that there is a highly positive relationship between GAS size and its mortality. Generally, GAS mortality increased as it increased in size. This could be clearly observed in netbags than in the plastic trays. In the plastic trays, there was a very high mortality for the small-sized GAS, which was probably due to the lack of aeration and low
35
air exchange. No consistent patterns were observed between GAS mortality and its sex. A rice cropping calendar was suggested in order to sustain high yields and reduce GAS damage. Conclusions GAS mortality in terms of their size and sex ranged from 5-60%, over the observation periods. These values are very low conforming their fast reproductive potentials and growth. Thus, it is advised to initiate GAS management options even before land preparation, rather than during transplanting only. In addition, it is much harder to manage GAS once rice seedlings are already planted. A well-leveled field with proper water management is the key to reduce GAS damage to rice.
Recommendations GAS mortality rates should be studied in terms of its weight, weight loss and other factors related over a longer period. These studies would then help to fill the knowledge gaps on GAS management and provide refinement to the GAS management systems in rice ecosystems.
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Lansangan, V. A., A. G. Cagauan and M. Tayamen. 2002. Golden apple snail Pomacea canaliculata Lam. As feed for freshwater prawn Macrobrachium rosenbergii de Man. Poster paper 7th ICMAM, Los Baos, Laguna. Philippines. Litsinger, J. A. and D. B. Estano. 1993. Management of the GAS (Pomacea canaliculata Lamarck) in rice. Crop Prot. 12(5): 363-370. Medrano, F. G., B. L. Canapi, F. M. J. Olivares, V. C. Sagun, G. F. Estoy, J. S. Rosario, P. Pacada, S. R. Obien, and D. G. Bottrell. 1993. Barangay IPM Project in irrigated rice in Nueva Ecija, Philippines, maximizing the need for wet season insecticides treatments. Jt. Report of PhilRice, IRRI, and DA, and FAO, Manila, IRRI. 22pp. Mochida, O. 1988. Non-seedborne rice pests of quarantine importance, pp. 117-129. In: Rice seed health. IRRI, Manila. Okuma, M., K. Tanaka, and S. Sudo. 1994. Weed control method using apple snail (Pomacea canaliculata) in paddy fields. Weed Research, 39: 114-119. Oya, S. , Y. Hirai and Y. Miyahara. 1987. Overwintering of the apple snail. (Unknown Publication) Reazo, D. M. 1988. The suitability of snail (Pomacea sp.) meal as substitute for fishmeal in diets of cage-cultured Nile tilapia (Oreochromis niloticus) at three stocking density levels. Thesis (MS Biology) De La Salle Univ., Manila (Philippines). 83p. Revilla, I. M. G. F. Estoy, and F. V. Salazar. 1991. State of golden Kuhol infestation in selected rice farming villages in Nueva Ecija. IPM Network, Report of a workshop on the management of golden snail in the Philippines, p. 38-59. Rondon, M. B and D. P. Callo, Jr. 1989. Distribution and mode of infestation of golden snail in rice farming, p. 12. In: B. O. Acosta and R. S. V. Pullin (eds.). Environmental impact of the golden apple snail (Pomacea spp.) on rice farming system in the Philippines. ICLARM, Manila, Philippines. Saxena, R. C., A. V. de Lara and H. D. Justo. 1987. Golden apple snail: a pest of rice. Int. Rice Res. Newsl. 12 (1): 24-25. 40
Syobu, S., H. Mikuriya, J. Yamajuchi and M. Matsuzaki. 2001. Estimating the overwintering mortality of the apple snail in a paddy field of southern Japan using temperature data. (Unknown Publication) Tacio HD, 1987. Raise snails for your ducks. Agribusiness Weekly, Philippines. 6-12 November, p. 16-17. Takahashi K, Seki M, Nishida H, 2002a. Research for control of apple snail by rotary cultivator. Journal of the Japanese Society of Agricultural Machinery, 64: 76-81. Takahashi K, Seki M, Nishida H, 2002b. Prevention of the harm from apple snail with rotary cultivator. Journal of the Japanese Society of Agricultural Machinery, 64: 101-107. Wada, T., R. C. Joshi and Y. Yusa. 2002. Experiences of Japanese rice farmers with apple snail, Pomacea canaliculata (Lamarck) for paddyweeding in transplanted rice: A video Documentation. Paper presented at the Seventh International Congress on Medical and Allied Malacology, 21-24 October 2002, Los Baos, Laguna, Philippines, 22 p. Yusa, Y., T. Wada and K. Takahashi. 2003. Apple snails in Japan: Their problems, Control strategies and possible benefit. Paper presented at the Korea-Japan Joint Conference on Applied Entomology and Zoology, 28-31 May, 2003, Grand Hotel, Haeundae, Busan, South Korea, 105 p.
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Web Links The Global Invasive Species Dat abase from New Zealand ISSG Website: http://www.issg.org/ http://www.issg.org/database/welcome/ Database on Introductions of Aquatic Species (DIAS) http://www.fao.org/waicent/faoinfo/fishery/statist/fisoft/dias/mainpage.h tm The golden apple snail in the rice fields of Asia http://www.fao.org/NEWS/1998/RIFILI -E.HTM Sarawak Warns Of Snail Threat http://agrolink.moa.my/moa1/newspaper/pertanian/ra981123c.html No. 1 GOLDEN APPLE SNAIL IN PAPUA NEW GUINEA Papua New Guinea has ... (PDF) http://www.spc.int/pps/PestAlerts/AgAlertNo01.pdf view as html N PAG DATA: POMACEA CANALICULATA GOLDEN APPLE SNAIL (PDF) http://www.cphst.org/npag/Molamppc598.pdf http://www.state.gov/g/oes/ocns/inv/cs/2312.htm http://pi.cdfa.ca.gov/advisory/gold_98.htm http://www.knowledgebank.irri.org/troprice/golden_apple_snail.ht m Socio-Economic Aspects of Biological Invasion, A Case Study: the Golden Apple Snail http://www.gcrio.org/ASPEN/science/eoc94/EOC3/EOC3-18.html http://www.abc.net.au/rn/science/earth/stories/s108695.htm http://www.aquahobby.com/gallery/gpbridgesi.html http://www.cat1234.com/id137.htm http://www.ricecrc.org/reader/golden_apple_snail.htm http://www.rdi.ku.ac.th/Manual_Res45/prominent_researchY44-45/p3941.pdf 42
USE OF ROTTEN JACKFRUIT TO CONTROL GOLDEN APPLE SNAIL http://www.agnet.org/library/article/pt2002041.html Eco-Jobs & Eco-Entrepreneurship A Global Data base on such Initiatives & Opportunities http://www.mssrf.org/ecojobs/sard/175b.html HALTING THE SNAIL TRAIL OF DESTRUCTION http://www.csiro.au/news/mediarel/mr1998/mr98241.html http://www.gsmfc.org/nis/nis/Pomacea _canaliculata.html The Florida (USA) link to golden apple snail is as follows: http://www.floridaaquaculture.com/Pub/Pub.htm The apple snail technical bulletin http://www.floridaaquaculture.com/Pub/Apple%20Snails.pdf Apple Snails in Wetland Taro Production http://agrss.sherman.hawaii.edu/onfarm/pest/pest0008.html Aquatic Species Introductions Database from FAO http://www.fao.org/scripts/acqintro/query/retrive.idc MISSISSIPPI EMERGENCY APPLE SNAIL REGULATION http://doacs.state.fl.us/~pi/plantinsp/AppleSnailMainx.html Some Hawaii pests arrived by invitation http://www.botany.hawaii.edu/botany/news/applesnail.htm Un sudamericano invade Asia http://www.ciencia-hoy.retina.ar/hoy66/sudamericano.htm http://konarc.naro.affrc.go.jp/kiban/g_seitai/hmpgsctn.html The Apple snail website http://www.applesnail.net http://www.applesnail.net \content\pest_alert\pest_alert.htm http://www.applesnail.net \content\pest_alert\asian_distribution\asian_di stribution.htm http://www.applesnail.net \pestalert\asian_pest_alert_poster\asian_pest_ alert_poster.htm 43
http://www.applesnail.net \pestalert\asian_pest_alert_poster\pest_alert_ poster.pdf http://www.applesnail.net \pestalert\management_guide\pest_manage ment.php http://www.applesnail.net \content\pest_alert\grass_hopper\grass_hopp er.htm The ECOPORT website http://www.ecoport.org/EP.exe$PictShow?ID=35024 CGIAR-SPIPM website (www.spipm.cgiar.org) http://www.runetwork.de/contribution.php?location=SPIPM_Interactive&l anguage=english&cid=1755 OPEN ACADEMY, PHILIPPINES Website www.openacademy.ph/elearning/goldenkohol/
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Appendices Experiment 1. GAS Mortality of Different GAS Sizes in Netbag Treatment Appendix 1. Weekly observation on the mortality of 10-mm GAS (November 21, 2004).
Snail No. 22 138 20 288 161 124 260 306 293 319 Initial Weight 0.34 0.33 0.37 0.32 0.31 0.27 0.39 0.25 0.38 0.4 Present Weight 0.17 0.29 0.32 0.27 0.26 0.24 0.34 0.19 0.33 0.34 Total Weight Loss 0.07 0.04 0.05 0.05 0.05 0.03 0.05 0.06 0.05 0.06 Status Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 20.59 12.12 13.51 15.63 16.13 11.11 12.82 24.00 13.16 15.00
Appendix 2. Weekly observation on the mortality of 10-mm GAS (December 4, 2004).

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Snail No. 299 229 10 99 218 268 56 228 281 311a Initial Weight 0.11 0.26 0.48 0.24 0.33 0.37 0.35 0.47 0.25 0.29 Present Weight 0.09 0.17 0.38 0.22 0.25 0.26 0.26 0.37 0.17 0.23 Total Weight Loss 0.02 0.09 0.1 0.02 0.08 0.11 0.09 0.1 0.08 0.06 Status Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 18.18 34.62 20.83 8.33 24.24 29.73 25.71 21.28 32.00 20.69
Appendix 4. Final observation on the mortality of 10-mm GAS (December 31, 2004).
Snail No. 116 142 198 267 283 313 318 25 39 41 42 52 57 60 62 68 74 76 81 85 87 91 94 95 99 100 111 Initial Weight 0.25 0.15 0.31 0.24 0.36 0.19 0.4 0.36 0.32 0.38 0.36 0.37 0.33 0.39 0.27 0.33 0.36 0.41 0.32 0.4 0.38 0.37 0.34 0.27 0.24 0.33 0.33 Present Weight 0.12 0.11 0.19 0.11 0.08 0.05 0.19 0.29 0.25 0.16 0.27 0.28 0.25 0.27 0.18 0.27 0.28 0.3 0.24 0.29 0.27 0.26 0.26 0.21 0.16 0.2 0.23 Total Weight Loss 0.13 0.04 0.12 0.13 0.28 0.14 0.21 0.07 0.07 0.22 0.09 0.09 0.08 0.12 0.09 0.06 0.08 0.11 0.08 0.11 0.11 0.11 0.08 0.06 0.08 0.13 0.1 Status Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 52.00 26.67 38.71 54.17 77.78 73.68 52.50 19.44 21.88 57.89 25.00 24.32 24.24 30.77 33.33 18.18 22.22 26.83 25.00 27.50 28.95 29.73 23.53 22.22 33.33 39.39 30.30
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112 113 118 119 123 152 154 166 167 170 173 177 179 186 192 201 204 206 216 217 219 224 236 238 242 252 270 271 282 289 290 291 295 298 303 312 316 324 330 337 311b 51a 51b
0.46 0.27 0.32 0.37 0.36 0.27 0.44 0.32 0.2 0.36 0.36 0.37 0.31 0.31 0.38 0.35 0.42 0.33 0.32 0.35 0.25 0.4 0.29 0.3 0.44 0.34 0.27 0.29 0.31 0.32 0.35 0.28 0.35 0.33 0.32 0.25 0.36 0.25 0.26 0.22 0.24 0.45 0.35
0.35 0.17 0.22 0.28 0.28 0.19 0.37 0.22 0.13 0.27 0.25 0.25 0.2 0.2 0.3 0.25 0.33 0.23 0.23 0.27 0.17 0.27 0.21 0.22 0.25 0.25 0.16 0.21 0.21 0.24 0.28 0.19 0.21 0.24 0.22 0.15 0.28 0.19 0.17 0.1 0.22 0.36 0.23
0.11 0.1 0.1 0.09 0.08 0.08 0.07 0.1 0.07 0.09 0.11 0.12 0.11 0.11 0.08 0.1 0.09 0.1 0.09 0.08 0.08 0.13 0.08 0.08 0.19 0.09 0.11 0.08 0.1 0.08 0.07 0.09 0.14 0.09 0.1 0.1 0.08 0.06 0.09 0.12 0.02 0.09 0.12
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
23.91 37.04 31.25 24.32 22.22 29.63 15.91 31.25 35.00 25.00 30.56 32.43 35.48 35.48 21.05 28.57 21.43 30.30 28.13 22.86 32.00 32.50 27.59 26.67 43.18 26.47 40.74 27.59 32.26 25.00 20.00 32.14 40.00 27.27 31.25 40.00 22.22 24.00 34.62 54.55 8.33 20.00 34.29
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Appendix 5. Weekly observation on the mortality of 15-mm GAS (November 21, 2004).
Snail No. 315 94 186 46 63 141 382 237 139 168 Initial Weight 0.83 0.61 0.47 1 0.59 0.74 0.77 0.64 0.47 0.63 Present Weight 0.75 0.55 0.41 0.94 0.53 0.67 0.7 0.56 0.42 0.58 Total Weight Loss 0.08 0.06 0.06 0.06 0.06 0.07 0.07 0.08 0.05 0.05 Status Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 9.64 9.84 12.77 6.00 10.17 9.46 9.09 12.50 10.64 7.94

Snail No. 198 199 383 9 311 225 83 92 365 7 Initial Weight 0.43 0.48 0.78 0.9 0.8 0.88 0.91 0.72 0.63 0.75 Present Weight 0.34 0.36 0.63 0.75 0.69 0.75 0.75 0.62 0.5 0.61 Total Weight Loss 0.09 0.12 0.15 0.15 0.11 0.13 0.16 0.1 0.13 0.14 Status Weight Loss % Alive 20.93 Alive 25.00 Alive 19.23 Alive 16.67 Alive 13.75 Alive 14.77 Alive 17.58 Alive 13.89 Alive 20.63 Alive 18.67
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Snail No. 37 45 96 299 3 4 8 14 16 20 31 33 38 39 58 65 72 87 108 110 111 134 136 144 145 146 149 151 159 165 166 177 182 183 188 197 200 202 206 212 216 219 222 Initial Weight 0.82 0.71 0.69 0.32 1.12 0.91 0.62 0.71 1.12 0.48 0.64 0.55 0.71 0.74 0.89 0.85 0.7 0.81 0.8 0.65 0.77 0.5 0.62 0.7 0.95 0.85 0.6 0.72 0.7 0.66 0.78 0.45 0.62 0.82 0.64 0.81 0.43 0.5 0.54 0.55 0.53 0.43 0.61 Present Weight 0.3 0.38 0.23 0.21 0.79 0.79 0.5 0.6 0.83 0.38 0.52 0.4 0.58 0.62 0.79 0.64 0.47 0.6 0.73 0.43 0.59 0.36 0.51 0.52 0.77 0.62 0.41 0.55 0.55 0.46 0.62 0.35 0.48 0.68 0.5 0.67 0.28 0.3 0.43 0.45 0.43 0.28 0.47 Total Weight Loss 0.52 0.33 0.46 0.11 0.33 0.12 0.12 0.11 0.29 0.1 0.12 0.15 0.13 0.12 0.1 0.21 0.23 0.21 0.07 0.22 0.18 0.14 0.11 0.18 0.18 0.23 0.19 0.17 0.15 0.2 0.16 0.1 0.14 0.14 0.14 0.14 0.15 0.2 0.11 0.1 0.1 0.15 0.14 Status Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 63.41 46.48 66.67 34.38 29.46 13.19 19.35 15.49 25.89 20.83 18.75 27.27 18.31 16.22 11.24 24.71 32.86 25.93 8.75 33.85 23.38 28.00 17.74 25.71 18.95 27.06 31.67 23.61 21.43 30.30 20.51 22.22 22.58 17.07 21.88 17.28 34.88 40.00 20.37 18.18 18.87 34.88 22.95
49
250 252 261 277 281 290 292 293 302 317 322 325 330 338 340 357 364 374 378 379 396 407 411 416 419 446 447
0.54 0.82 0.81 0.58 0.39 0.77 0.62 0.81 0.81 0.81 0.63 0.77 0.37 0.8 0.58 0.49 0.63 0.79 0.87 0.71 0.48 0.52 0.75 0.6 0.68 0.39 0.34
0.46 0.66 0.63 0.42 0.27 0.65 0.24 0.64 0.64 0.63 0.51 0.64 0.29 0.62 0.37 0.38 0.49 0.66 0.67 0.54 0.35 0.37 0.61 0.41 0.56 0.26 0.21
0.08 0.16 0.18 0.16 0.12 0.12 0.38 0.17 0.17 0.18 0.12 0.13 0.08 0.18 0.21 0.11 0.14 0.13 0.2 0.17 0.13 0.15 0.14 0.19 0.12 0.13 0.13
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
14.81 19.51 22.22 27.59 30.77 15.58 61.29 20.99 20.99 22.22 19.05 16.88 21.62 22.50 36.21 22.45 22.22 16.46 22.99 23.94 27.08 28.85 18.67 31.67 17.65 33.33 38.24
Snail No. 264 127 149 221 216 307 296 130 180 155 Sex f f f f f f f f f m Initial Weight 1.43 1.58 1.32 1.43 1.55 1.39 1.66 1.36 1.44 1.86 Present Weight 1.29 0.98 1.17 1.35 1.4 1.27 1.52 1.23 1.3 1.73 Total Weight Loss 0.14 0.6 0.15 0.08 0.15 0.12 0.14 0.13 0.14 0.13 Status Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 9.79 37.97 11.36 5.59 9.68 8.63 8.43 9.56 9.72 6.99
50
Snail No. 193 270 47 220 73 231 135 271 215 308 Sex f f f f f f f f f m Initial Weight 1.7 1.28 1.33 1.6 1.71 1.45 1.54 1.5 2.06 1.52 Present Weight 1.41 1.24 1.2 1.29 1.52 1.17 1.39 1.35 1.61 1.11 Total Weight Loss 0.29 0.04 0.13 0.31 0.19 0.28 0.15 0.15 0.45 0.41 Status Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 17.06 3.13 9.77 19.38 11.11 19.31 9.74 10.00 21.84 26.97
Snail No. 41 19 260 43 61 69 28 32 304 30 Initial Weight 1.58 1.67 1.31 1.54 1.5 1.62 1.45 1.38 1.36 1.65 Present Weight 0.61 0.72 1.08 1.26 1.2 1.26 1.18 1.18 1.09 1.42 Total Weight Loss 0.97 0.95 0.23 0.28 0.3 0.36 0.27 0.2 0.27 0.23 Weight Loss % 61.39 56.89 17.56 18.18 20.00 22.22 18.62 14.49 19.85 13.94
Sex f f f f f f f f f m
Status Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive
Snail No. 123 145 146 188 211 244 253 263 283 Sex f f f f f f f f f Initial Weight 1.07 1.7 1.74 1.17 1.35 1.2 1.62 1.3 1.88 Present Weight 0.61 0.78 1.4 0.87 0.98 0.6 0.84 0.77 0.66 Total Weight Loss 0.46 0.92 0.34 0.3 0.37 0.6 0.78 0.53 1.22 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Weight Loss % 42.99 54.12 19.54 25.64 27.41 50.00 48.15 40.77 64.89
51
289 2 5 10 13 24 25 33 44 49 53 68 72 77 78 79 80 119 120 121 122 125 134 144 147 151 156 163 166 169 177 178 181 185 186 197 198 199 212 213 226 233 242 250 254 257 273
f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f
1.02 1.58 1.28 1.91 1.69 1.49 1.5 2 1.42 1.49 1.6 1.61 1.82 1.81 1.26 1.44 1.18 1.28 1.64 1.24 2.06 1.7 1.55 1.21 1.56 1.91 1.54 1.8 1.71 2.03 1.25 1.35 1.41 1.36 1.35 1.26 1.73 1.46 1.29 1.6 1.66 1.15 1.64 1.69 1.64 2.14 1.4
0.7 1.26 1.04 1.53 1.31 1.15 1.13 1.6 1.14 1.21 1.23 1.38 1.56 1.55 1.03 1.16 0.91 0.95 1.14 1 1.72 1.26 1.2 0.95 1.22 1.52 1.22 1.43 1.37 1.62 0.93 1.11 1.14 1.01 1.11 1.04 1.42 1.18 1.05 1.27 1.29 0.89 1.16 1.41 1.39 1.78 1.07
0.32 0.32 0.24 0.38 0.38 0.34 0.37 0.4 0.28 0.28 0.37 0.23 0.26 0.26 0.23 0.28 0.27 0.33 0.5 0.24 0.34 0.44 0.35 0.26 0.34 0.39 0.32 0.37 0.34 0.41 0.32 0.24 0.27 0.35 0.24 0.22 0.31 0.28 0.24 0.33 0.37 0.26 0.48 0.28 0.25 0.36 0.33
Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
31.37 20.25 18.75 19.90 22.49 22.82 24.67 20.00 19.72 18.79 23.13 14.29 14.29 14.36 18.25 19.44 22.88 25.78 30.49 19.35 16.50 25.88 22.58 21.49 21.79 20.42 20.78 20.56 19.88 20.20 25.60 17.78 19.15 25.74 17.78 17.46 17.92 19.18 18.60 20.63 22.29 22.61 29.27 16.57 15.24 16.82 23.57
52
277 284 288 302 305 317 318 319 322 18 34 179 195 219
f f f f f f f f f m m m m m
1.63 1.47 1.63 1.92 1.65 1.39 1.42 1.37 1.53 1.93 1.73 1.43 1.98 1.73
1.33 1.2 1.33 1.41 1.37 1.13 1.16 1.12 1.19 1.58 1.44 1.1 1.6 1.32
0.3 0.27 0.3 0.51 0.28 0.26 0.26 0.25 0.34 0.35 0.29 0.33 0.38 0.41
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
18.40 18.37 18.40 26.56 16.97 18.71 18.31 18.25 22.22 18.13 16.76 23.08 19.19 23.70
Snail No. 323 341 334 206 235 173 405 177 424 373 Sex f f f f f m m m m m Initial Weight 2.36 2.57 3.18 3.19 3 2.57 2.8 3.78 2.6 3.09 Present Weight 2.09 2.35 2.82 2.89 2.52 2.1 2.58 3.42 2.44 2.85 Total Weight Loss 0.27 0.22 0.36 0.3 0.48 0.47 0.22 0.36 0.16 0.24 Status Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 11.44 8.56 11.32 9.40 16.00 18.29 7.86 9.52 6.15 7.77
Snail No. 277 295 27 318 269 165 356 384 246 409 Initial Weight 1.7 3.68 3.01 2.92 2.94 3.25 3.29 3 2.33 2.29 Present Weight 0.99 1.79 2.61 2.61 2.56 2.87 2.79 2.72 1.83 1.52 Total Weight Loss 0.71 1.89 0.4 0.31 0.38 0.38 0.5 0.28 0.5 0.77 Weight Loss % 41.76 51.36 13.29 10.62 12.93 11.69 15.20 9.33 21.46 33.62
Sex f f f f f f f f f m
Status Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive
53
Snail No. 168 262 44 121 123 303 394 313 61 407 Sex f f f f f f f m m m Initial Weight 3.05 2.94 3.2 3.54 4.15 2.58 2.75 2.87 3.56 3.3 Present Weight 2.64 2.59 2.85 3.11 3.3 2.17 2.38 2.24 3.14 2.84 Total Weight Loss 0.41 0.35 0.35 0.43 0.85 0.41 0.37 0.63 0.42 0.46 Status Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 13.44 11.90 10.94 12.15 20.48 15.89 13.45 21.95 11.80 13.94
Snail No. 34 59 92 155 223 254 282 316 332 333 362 363 371 78 98 149 320 421 427 1 26 29 47 51 52 63 65 Sex f f f f f f f f f f f f f m m m m m m f f f f f f f f Initial Weight 2.45 3.09 2.54 3.15 3.36 3.37 3.2 2.81 3.27 2.94 2.71 1.27 2.62 2.74 2.9 2.58 2.43 2.38 2.18 3.61 3.37 3.37 2.63 3.21 3.05 3.42 3.2 Present Weight 0.87 1.01 1.03 2.67 1.11 1.7 1.01 1.38 0.93 1 1.17 0.78 1.4 1.22 0.96 1.27 1.34 1.03 1.22 3.15 2.71 2.94 2.07 2.36 2.56 2.94 2.48 Total Weight Loss 1.58 2.08 1.51 0.48 2.25 1.67 2.19 1.43 2.34 1.94 1.54 0.49 1.22 1.52 1.94 1.31 1.09 1.35 0.96 0.46 0.66 0.43 0.56 0.85 0.49 0.48 0.72 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 64.49 67.31 59.45 15.24 66.96 49.55 68.44 50.89 71.56 65.99 56.83 38.58 46.56 55.47 66.90 50.78 44.86 56.72 44.04 12.74 19.58 12.76 21.29 26.48 16.07 14.04 22.50
54
70 97 100 103 131 134 136 137 140 154 161 164 166 175 181 187 195 205 208 219 226 253 263 265 267 270 272 298 338 339 340 344 359 379 381 388 236 17 122 151 264 288 428
f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f f m m m m m m
3 2.89 2.67 3.17 2.81 2.89 2.76 3.1 3.27 2.52 2.6 2.45 3.35 2.86 2.88 2.46 3 3.1 3.24 3.49 3.17 2.88 2.93 2.43 2.95 3.06 2.85 2.74 3.24 3.15 3.07 2.47 2.39 3.03 3.4 3.44 2.9 2.7 3.59 3.25 3.08 2.97 2.69
2.4 2.44 2.05 2.45 2.35 2.51 2.1 2.36 2.87 2.03 2.27 1.88 2.82 2.35 3.49 1.92 2.46 2.69 2.72 2.84 2.43 2.01 2.56 1.9 2.18 2.65 2.41 2.12 2.72 2.44 1.9 1.93 1.92 2.61 2.76 2.95 2.32 1.98 3 2.68 2.56 2.49 2.41
0.6 0.45 0.62 0.72 0.46 0.38 0.66 0.74 0.4 0.49 0.33 0.57 0.53 0.51 -0.61 0.54 0.54 0.41 0.52 0.65 0.74 0.87 0.37 0.53 0.77 0.41 0.44 0.62 0.52 0.71 1.17 0.54 0.47 0.42 0.64 0.49 0.58 0.72 0.59 0.57 0.52 0.48 0.28
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
20.00 15.57 23.22 22.71 16.37 13.15 23.91 23.87 12.23 19.44 12.69 23.27 15.82 17.83 -21.18 21.95 18.00 13.23 16.05 18.62 23.34 30.21 12.63 21.81 26.10 13.40 15.44 22.63 16.05 22.54 38.11 21.86 19.67 13.86 18.82 14.24 20.00 26.67 16.43 17.54 16.88 16.16 10.41
55
Snail No. 271 360 404 42 14 16 239 285 65 354 Sex f f f f f f m m m m Initial Weight 7.31 7.73 8.03 5.75 7.14 5.69 7.29 7.04 6.32 6.91 Present Weight 4.71 3.37 3.48 4.99 3.65 3.67 5.08 3.83 4.23 4.27 Total Weight Loss 2.6 4.36 4.55 0.76 3.49 2.02 2.21 3.21 2.09 2.64 Status Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 35.57 56.40 56.66 13.22 48.88 35.50 30.32 45.60 33.07 38.21
Snail No. 259 170 305 72 215 312 338 335 81 384 Sex f f m f f f f f m m Initial Weight 4.35 4.42 4.01 5.62 6.4 5.16 4.05 4.59 6.13 3.72 Present Weight 2.47 2.92 2.18 5.21 5.52 4.72 3.28 4.07 5.79 3.17 Total Weight Loss 1.88 1.5 1.83 0.41 0.88 0.44 0.77 0.52 0.34 0.55 Status Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Weight Loss % 43.22 33.94 45.64 7.30 13.75 8.53 19.01 11.33 5.55 14.78
Snail No. 361 257 189 235 50 11 33 191 266 64 Sex f m m m m f f f f f Initial Weight 4.62 4.71 5.72 5.64 4.25 5.31 5.34 4.42 4.87 4.83 Present Weight 4.12 2.13 2.21 1.94 1.92 4.8 5.05 3.55 4.29 4.23 Total Weight Loss 0.5 2.58 3.51 3.7 2.33 0.51 0.29 0.87 0.58 0.6 Status Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Weight Loss % 10.82 54.78 61.36 65.60 54.82 9.60 5.43 19.68 11.91 12.42
56
Snail No. 15 86 126 134 139 145 226 332 341 365 132 137 159 252 396 407 6 17 20 22 24 27 35 37 40 47 48 49 67 88 111 115 118 143 155 188 197 209 213 222 241 258 Sex f f f f f f f f f f m m m m m m f f f f f f f f f f f f f f f f f f f f f f f f f f Initial Weight 4.78 4.13 4.98 4.32 5.33 5.27 3.87 4.46 5.18 4.72 4.99 4.91 4.41 4.26 4.59 4.57 4.9 4.49 5.17 4.2 5.56 5.48 4.89 4.95 5.54 4.5 5.15 4.97 4.84 5.26 4.91 5.12 5.18 4.05 4.13 5.51 6.36 5.12 4.48 4.58 5.22 6.8 Present Weight 1.9 1.33 1.5 3.23 2.51 2.51 2.36 1.92 1.64 2.54 1.66 2.82 1.63 1.82 1.6 2.49 3.61 3.62 4.43 3.4 4.54 4.69 3.99 4.2 4.43 3.79 4.35 3.09 4.12 4.47 4.26 4.48 3.56 3.48 3.07 4.59 5.65 4.22 3.68 3.73 4.65 5.6 Total Weight Loss 2.88 2.8 3.48 1.09 2.82 2.76 1.51 2.54 3.54 2.18 3.33 2.09 2.78 2.44 2.99 2.08 1.29 0.87 0.74 0.8 1.02 0.79 0.9 0.75 1.11 0.71 0.8 1.88 0.72 0.79 0.65 0.64 1.62 0.57 1.06 0.92 0.71 0.9 0.8 0.85 0.57 1.2 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 60.25 67.80 69.88 25.23 52.91 52.37 39.02 56.95 68.34 46.19 66.73 42.57 63.04 57.28 65.14 45.51 26.33 19.38 14.31 19.05 18.35 14.42 18.40 15.15 20.04 15.78 15.53 37.83 14.88 15.02 13.24 12.50 31.27 14.07 25.67 16.70 11.16 17.58 17.86 18.56 10.92 17.65
57
272 278 283 286 290 310 348 359 368 371 375 376 1 92 108 135 148 167 202 214 244 248 254 284 299 316 387 403
f f f f f f f f f f f f m m m m m m m m m m m m m m m m
5.32 5.12 5.52 6.3 4.95 5.86 4.98 5.51 4.52 4.99 6.15 5.36 5.88 5.66 5.04 4.3 4.84 5.39 4.37 4.36 4.47 4.2 4.59 4.87 5.19 4.5 5.61 4.99
4.67 4.09 4.6 5.56 4.08 5.34 3.57 4.67 3.6 3.92 5.44 4.67 4.96 4.91 3.76 3.14 3.68 4.7 3.26 2.81 3.87 2.93 3.22 4.28 4.67 3.55 4.66 4.52
0.65 1.03 0.92 0.74 0.87 0.52 1.41 0.84 0.92 1.07 0.71 0.69 0.92 0.75 1.28 1.16 1.16 0.69 1.11 1.55 0.6 1.27 1.37 0.59 0.52 0.95 0.95 0.47
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
12.22 20.12 16.67 11.75 17.58 8.87 28.31 15.25 20.35 21.44 11.54 12.87 15.65 13.25 25.40 26.98 23.97 12.80 25.40 35.55 13.42 30.24 29.85 12.11 10.02 21.11 16.93 9.42
Snail No. 46 380 413 170 144 381 342 388 82 450 Sex f f f f f f m m m m Initial Weight 6.66 6.9 5.35 6.39 8.5 7.7 7.56 5.82 6.68 7.05 Present Weight 5.85 4.73 3.95 5.81 8.07 7.02 7.16 5.34 6.23 6.32 Total Weight Loss 0.81 2.17 1.4 0.58 0.43 0.68 0.4 0.48 0.45 0.73 Status Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Weight Loss % 12.16 31.45 26.17 9.08 5.06 8.83 5.29 8.25 6.74 10.35
58
Initial Weight 4.71 5.4 5.89 8.41 8.01 7.71 6.36 9.07 6.36 5.64 Present Weight 3.21 2.9 2.02 7.31 7.52 6.93 5.18 8.62 5.91 4.67 Total Weight Loss 1.5 2.5 3.87 1.1 0.49 0.78 1.18 0.45 0.45 0.97 Weight Loss % 31.85 46.30 65.70 13.08 6.12 10.12 18.55 4.96 7.08 17.20
Snail No. 303 301 215 437 210 10 423 53 203 221
Sex f f m f f f f f m m
Status Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive
Snail No. 246 427 325 392 412 176 434 288 452 321 Sex m m f f f f m m m m Initial Weight 6 5.22 5.53 7.12 6.13 5.39 4.76 5.22 7.24 5.86 Present Weight 2.12 1.65 4.44 5.72 4.29 4.49 3.51 4.18 5.93 4.75 Total Weight Loss 3.88 3.57 1.09 1.4 1.84 0.9 1.25 1.04 1.31 1.11 Status Weight Loss % Dead 64.67 Dead 68.39 Alive 19.71 Alive 19.66 Alive 30.02 Alive 16.70 Alive 26.26 Alive 19.92 Alive 18.09 Alive 18.94
Snail No. 23 76 80 105 212 226 230 355 365 438 Sex f f f f f f f f f f Initial Weight 5.31 7.12 7.14 7.07 4.44 6.49 6 6.25 6.68 5.59 Present Weight 1.2 2.84 3.49 4.29 2.27 2.33 3.49 3.3 3.75 1.47 Total Weight Loss 4.11 4.28 3.65 2.78 2.17 4.16 2.51 2.95 2.93 4.12 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Weight Loss % 77.40 60.11 51.12 39.32 48.87 64.10 41.83 47.20 43.86 73.70
59
31 156 169 205 235 253 352 367 396 422 8 22 51 100 146 183 264 290 291 318 330 335 347 348 361 401 26 47 48 59 60 74 93 96 107 141 149 167 202 204 207 213 249 250 286 314 334 350
m m m m m m m m m m f f f f f f f f f f f f f f f f m m m m m m m m m m m m m m m m m m m m m m
7.18 6.71 8.19 5.55 4.91 5.9 4.73 5.4 7.98 6.14 7.21 8.76 5.36 6.65 5.87 5.74 5.88 6.95 6.94 5.37 7.96 6.14 7.74 6.4 6.72 6.83 7.05 6.25 6.16 7.89 6.28 7.31 7.05 6.82 6.95 5.89 7.56 9.68 6.46 5.73 5.82 6.35 6.3 6.99 7.1 6.27 5.59 6.5
3.68 2.08 3.26 3.46 1.59 1.86 2.64 1.61 4.16 3.32 6.15 8 4.32 5.29 5.03 4.68 4.97 6.27 5.01 4.33 7.09 4.55 6.86 4.25 6.1 5.14 5.71 5.04 5.12 7.37 5.33 6.42 5.86 5.78 5.22 4.35 4.36 8.92 5.32 3.73 4.81 5.35 4.99 5.67 5.36 5.11 4.1 5.03
3.5 4.63 4.93 2.09 3.32 4.04 2.09 3.79 3.82 2.82 1.06 0.76 1.04 1.36 0.84 1.06 0.91 0.68 1.93 1.04 0.87 1.59 0.88 2.15 0.62 1.69 1.34 1.21 1.04 0.52 0.95 0.89 1.19 1.04 1.73 1.54 3.2 0.76 1.14 2 1.01 1 1.31 1.32 1.74 1.16 1.49 1.47
Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
48.75 69.00 60.20 37.66 67.62 68.47 44.19 70.19 47.87 45.93 14.70 8.68 19.40 20.45 14.31 18.47 15.48 9.78 27.81 19.37 10.93 25.90 11.37 33.59 9.23 24.74 19.01 19.36 16.88 6.59 15.13 12.18 16.88 15.25 24.89 26.15 42.33 7.85 17.65 34.90 17.35 15.75 20.79 18.88 24.51 18.50 26.65 22.62
60
366 370 371 373 382 385 395 405 425 426 431 435 453
m m m m m m m m m m m m m
7.56 5.8 7.28 8.46 5.39 4.99 8.12 7.94 6.6 6.48 5.67 6.48 5.04
6.66 1.69 6.18 7.26 4.28 3.99 6.86 6.17 4.85 5.7 4.79 5.56 3.73
0.9 4.11 1.1 1.2 1.11 1 1.26 1.77 1.75 0.78 0.88 0.92 1.31
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
11.90 70.86 15.11 14.18 20.59 20.04 15.52 22.29 26.52 12.04 15.52 14.20 25.99
Snail No. 254 191 255 279 259 235 88 267 269 223 Sex f f f m f f m m m m Initial Weight 9.04 7.35 8.32 10.71 9.65 9.25 7.33 9.42 11.24 9.65 Present Weight 7.86 4.93 6.82 9.46 8.95 8.27 6.78 9 7.65 8.81 Total Weight Loss 1.18 2.42 1.5 1.25 0.7 0.98 0.55 0.42 3.59 0.84 Status Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Weight Loss % 13.05 32.93 18.03 11.67 7.25 10.59 7.50 4.46 31.94 8.70
Snail No. 17 86 159 210 202 12 263 212 315 242 Sex f f f m f f m m m m Initial Weight 8.87 6.56 7.69 7.99 8.63 7.78 7.73 10.03 14.25 9.36 Present Weight 3.42 2.96 6.21 3.07 6.91 6.32 6.56 8.65 13.24 8.6 Total Weight Loss 5.45 3.6 1.48 4.92 1.72 1.46 1.17 1.38 1.01 0.76 Status Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Weight Loss % 61.44 54.88 19.25 61.58 19.93 18.77 15.14 13.76 7.09 8.12
61
Snail No. 222 239 220 75 175 167 158 313 217 81 Sex f f m f f f f m m m Initial Weight 7.11 6.88 7.48 9.68 10.99 10.1 9.65 7.6 11.32 8.58 Present Weight 3.56 5.96 2.55 8.86 9.71 8.4 7.72 5.52 9.96 7.44 Total Weight Loss 3.55 0.92 4.93 0.82 1.28 1.7 1.93 2.08 1.36 1.14 Status Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Weight Loss % 49.93 13.37 65.91 8.47 11.65 16.83 20.00 27.37 12.01 13.29
Snail No. 3 11 25 45 56 60 64 83 84 87 166 206 224 239 247 258 5 16 37 52 62 215 219 248 250 273 276 Sex f f f f f f f f f f f f f f f f m m m m m m m m m m m Initial Weight 8.12 9.08 9.1 7.94 6.41 9.99 5.83 8.12 8.39 7.12 8.43 10.12 6.7 6.88 8.75 7.38 9.73 7.08 7.17 7.7 6.8 6.82 7.46 10.61 8.87 8.95 8.28 Present Weight 2.52 2.67 2.77 4.37 3.09 3.63 2.94 4.29 2.42 4.48 5.42 2.77 3.11 3.14 5.64 2.78 6.43 4.36 2.11 5.29 3.63 2.94 4.13 3.23 6.06 3.19 2.9 Total Weight Loss 5.6 6.41 6.33 3.57 3.32 6.36 2.89 3.83 5.97 2.64 3.01 7.35 3.59 3.74 3.11 4.6 3.3 2.72 5.06 2.41 3.17 3.88 3.33 7.38 2.81 5.76 5.38 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Weight Loss % 68.97 70.59 69.56 44.96 51.79 63.66 49.57 47.17 71.16 37.08 35.71 72.63 53.58 54.36 35.54 62.33 33.92 38.42 70.57 31.30 46.62 56.89 44.64 69.56 31.68 64.36 64.98
62
280 297 298 317 7 18 22 34 46 57 67 85 156 162 173 180 184 192 209 227 234 246 252 253 257 73 78 211 216 218 229 231 236 237 243 260 281 286 287 292 293 294 316
m m m m f f f f f f f f f f f f f f f f f f f f f m m m m m m m m m m m m m m m m m m
6.8 6.96 9.05 7.48 9.5 9.25 6.56 10.44 13.26 11.55 9.87 11.18 9.8 10.36 7.73 8.28 8.75 11.76 11.51 6.86 7.81 8.86 9.71 8.96 11.82 9.09 6.9 9.47 10.43 8.22 8.97 9.35 7.32 8.42 8.15 7.71 8.74 9.36 11.16 10.59 8.29 11.17 8.16
3.29 2.13 5.84 2.43 8.23 6.04 5.86 8.44 11.12 9.79 8.44 9.39 9.01 8.18 5.71 5.24 5.31 10.23 9.2 5.82 5.57 7.51 6.28 7.12 9.46 6.93 5.36 8.1 8.85 5.64 7.14 7.06 4.67 7.02 5.51 6.01 6.41 7.37 8.57 8.33 5.01 9.26 5.68
3.51 4.83 3.21 5.05 1.27 3.21 0.7 2 2.14 1.76 1.43 1.79 0.79 2.18 2.02 3.04 3.44 1.53 2.31 1.04 2.24 1.35 3.43 1.84 2.36 2.16 1.54 1.37 1.58 2.58 1.83 2.29 2.65 1.4 2.64 1.7 2.33 1.99 2.59 2.26 3.28 1.91 2.48
Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive
51.62 69.40 35.47 67.51 13.37 34.70 10.67 19.16 16.14 15.24 14.49 16.01 8.06 21.04 26.13 36.71 39.31 13.01 20.07 15.16 28.68 15.24 35.32 20.54 19.97 23.76 22.32 14.47 15.15 31.39 20.40 24.49 36.20 16.63 32.39 22.05 26.66 21.26 23.21 21.34 39.57 17.10 30.39
63
Experiment 2. GAS Mortality of Different GAS Sizes in Plastic Tray with Soil Treatment Appendix 29. Final observation on the mortality of various GAS sizes in plastic trays (December 31, 2004).
S. No 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 10 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive 15 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 20 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 25 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 30 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 35 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 40 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive
64
41 42 43 44 45 46 47 48 49 50 % Mortality
Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 70
Experiment 3. GAS Mortality of Different GAS Sexes in Netbag Treatment Appendix 30. Final observation on the mortality of 25-mm male GAS (December 31, 2004).
Snail No. 14 68 109 304 408 420 15 19 105 124 179 189 212 241 244 261 271 283 291 296 319 343 399 418 425 Initial Weight 2.61 2.21 2.38 2.32 2.7 2.16 3.54 3.29 3.2 2.54 3.02 3.18 3 3.09 2.59 3.25 4.03 2.95 2.91 3.27 2.63 2.85 3.04 2.75 2.54 Present Weight 1.58 1.35 1.05 1.42 1 0.76 2.85 2.67 2.43 1.91 2.17 2.43 2.6 2.23 2.13 2.02 3.49 2.35 2.44 2.37 1.76 2.16 2.25 2.22 1.87 Total Weight Loss 1.03 0.86 1.33 0.9 1.7 1.4 0.69 0.62 0.77 0.63 0.85 0.75 0.4 0.86 0.46 1.23 0.54 0.6 0.47 0.9 0.87 0.69 0.79 0.53 0.67 Status Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 39.46 38.91 55.88 38.79 62.96 64.81 19.49 18.84 24.06 24.80 28.15 23.58 13.33 27.83 17.76 37.85 13.40 20.34 16.15 27.52 33.08 24.21 25.99 19.27 26.38
65
Appendix 31. Final observation on the mortality of 25-mm female GAS (December 31, 2004).
Snail No. 115 194 211 273 326 6 11 13 30 67 69 86 117 141 142 183 186 250 275 346 347 355 378 389 393 Initial Weight 2.63 2.29 2.37 3.13 2.63 3.06 2.88 2.97 2.65 2.89 3.54 2.37 2.27 3.15 2.99 3.1 3.19 3.5 3.2 2.6 2.92 2.56 1.99 3.34 3.2 Present Weight 1.63 1.26 1.08 1.27 1.27 2.58 2.32 2.46 1.82 2.34 2.81 1.88 1.69 2.74 2.43 2.6 2.52 2.96 2.64 2.07 2.25 1.95 1.63 2.54 2.61 Total Weight Loss 1 1.03 1.29 1.86 1.36 0.48 0.56 0.51 0.83 0.55 0.73 0.49 0.58 0.41 0.56 0.5 0.67 0.54 0.56 0.53 0.67 0.61 0.36 0.8 0.59 Status Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 38.02 44.98 54.43 59.42 51.71 15.69 19.44 17.17 31.32 19.03 20.62 20.68 25.55 13.02 18.73 16.13 21.00 15.43 17.50 20.38 22.95 23.83 18.09 23.95 18.44
66
Appendix 32. Final observation on the mortality of 30-mm male GAS (December 31, 2004).
Snail No. 70 251 398 54 66 76 110 142 144 151 163 174 208 247 249 276 279 291 303 320 321 322 381 394 399 Initial Weight 4.59 4.26 3.04 4.9 4.86 4.57 5.68 4.92 4.81 5.43 5.4 4.92 5.42 4.18 4.63 5.97 5.54 4.46 5.49 5.54 6.39 4.28 4.41 5.02 6.26 Present Weight 1.66 1.64 1.75 3.97 4.11 3.72 4.87 3.85 3.69 4.76 3.69 4.2 4.46 3.12 3.32 4.88 4.27 3.14 4.8 4.63 5.5 3.4 3.59 3.75 5.22 Total Weight Loss 2.93 2.62 1.29 0.93 0.75 0.85 0.81 1.07 1.12 0.67 1.71 0.72 0.96 1.06 1.31 1.09 1.27 1.32 0.69 0.91 0.89 0.88 0.82 1.27 1.04 Status Weight Loss % Dead 63.83 Dead 61.50 Dead 42.43 Alive 18.98 Alive 15.43 Alive 18.60 Alive 14.26 Alive 21.75 Alive 23.28 Alive 12.34 Alive 31.67 Alive 14.63 Alive 17.71 Alive 25.36 Alive 28.29 Alive 18.26 Alive 22.92 Alive 29.60 Alive 12.57 Alive 16.43 Alive 13.93 Alive 20.56 Alive 18.59 Alive 25.30 Alive 16.61
67
Snail No. 2 52 277 340 349 353 12 59 62 68 101 105 114 123 149 184 198 201 216 225 273 326 327 350 351 Initial Weight 4.62 4.49 2.43 3.94 4.72 4.09 5.13 4.81 5.66 4.25 5.26 5.32 5.1 5.27 5.29 5.35 3.93 4.57 5.88 4.41 5.53 4.99 5.05 5.34 5.38 Present Weight 1.37 1.74 1.25 2.38 1.61 1.28 4.04 4.12 4.46 3.34 4.46 4.44 3.63 4.24 4.51 4.39 3.27 2.93 4.7 3.26 4.57 4.26 4.38 4.55 4.72 Total Weight Loss 3.25 2.75 1.18 1.56 3.11 2.81 1.09 0.69 1.2 0.91 0.8 0.88 1.47 1.03 0.78 0.96 0.66 1.64 1.18 1.15 0.96 0.73 0.67 0.79 0.66 Status Weight Loss % Dead 70.35 Dead 61.25 Dead 48.56 Dead 39.59 Dead 65.89 Dead 68.70 Alive 21.25 Alive 14.35 Alive 21.20 Alive 21.41 Alive 15.21 Alive 16.54 Alive 28.82 Alive 19.54 Alive 14.74 Alive 17.94 Alive 16.79 Alive 35.89 Alive 20.07 Alive 26.08 Alive 17.36 Alive 14.63 Alive 13.27 Alive 14.79 Alive 12.27
68
Snail No. 12 65 87 108 111 145 211 341 448 21 49 130 138 149 153 162 179 189 190 216 261 272 274 327 411 Initial Weight 6.63 6.32 6.91 7.06 7.59 7.03 5.5 5.65 6.86 5.4 5.24 8.02 7.78 7.56 8.82 7.33 6.1 5.77 5.89 4.82 6.62 7.39 4.75 5.69 5.83 Present Weight 3.45 2.18 3.42 5.17 2.99 3.12 2.75 3.57 2.01 3.65 4.18 6.47 7.04 6.35 7.67 6.24 5.94 4 4.52 3.47 5.27 6.46 3.03 3.96 4.97 Total Weight Loss 6.63 4.14 3.49 1.89 4.6 3.91 2.75 2.08 4.85 1.75 1.06 1.55 0.74 1.21 1.15 1.09 0.16 1.77 1.37 1.35 1.35 0.93 1.72 1.73 0.86 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 100.00 65.51 50.51 26.77 60.61 55.62 50.00 36.81 70.70 32.41 20.23 19.33 9.51 16.01 13.04 14.87 2.62 30.68 23.26 28.01 20.39 12.58 36.21 30.40 14.75
69
Snail No. 39 58 72 77 94 113 211 227 307 357 362 420 446 9 13 116 157 168 180 181 267 296 311 419 451 Initial Weight 4.19 9.17 4.7 6.65 6.39 8.12 5.5 5.24 6.61 6.09 6.92 5.65 7.03 6.21 7.29 8.12 5.52 8.02 5.77 6.12 6.4 8.65 6.87 6.15 5.98 Present Weight 1.9 3.09 2.48 4.12 4.62 3.5 3.01 1.51 2.49 3.53 3.72 2.48 2.35 5.23 6.25 5.5 4.19 6.85 3.73 4.42 4.89 6.37 5.06 5.33 3.88 Total Weight Loss 2.29 6.08 2.22 2.53 1.77 4.62 2.49 3.73 4.12 2.56 3.2 3.17 4.68 0.98 1.04 2.62 1.33 1.17 2.04 1.7 1.51 2.28 1.81 0.82 2.1 Status Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Weight Loss % 54.65 66.30 47.23 38.05 27.70 56.90 45.27 71.18 62.33 42.04 46.24 56.11 66.57 15.78 14.27 32.27 24.09 14.59 35.36 27.78 23.59 26.36 26.35 13.33 35.12
70
Snail No. 24 33 40 59 68 69 77 266 288 289 291 300 301 311 15 20 28 36 44 49 271 274 275 304 314 Initial Weight 7.55 7.26 8.93 6.69 4.83 8.89 8.52 7.86 10.01 7.09 8.95 8.49 7.96 12.13 8.7 8.27 8.74 7.94 9.2 5.91 11.57 10.07 9.62 13.56 9.61 Present Weight 2.25 4.9 3.04 4.22 2.16 3.22 4.41 2.4 4 4.31 4.86 2.55 5.27 3.18 7.4 6.53 7.37 5.97 7.8 4.7 10.28 9.07 8.13 11.98 7.93 Total Weight Loss 5.3 2.36 5.89 2.47 2.67 5.67 4.11 5.46 6.01 2.78 4.09 5.94 2.69 8.95 1.3 1.74 1.37 1.97 1.4 1.21 1.29 1 1.49 1.58 1.68 Status Weight Loss % Dead 70.20 Dead 32.51 Dead 65.96 Dead 36.92 Dead 55.28 Dead 63.78 Dead 48.24 Dead 69.47 Dead 60.04 Dead 39.21 Dead 45.70 Dead 69.96 Dead 33.79 Dead 73.78 Alive 14.94 Alive 21.04 Alive 15.68 Alive 24.81 Alive 15.22 Alive 20.47 Alive 11.15 Alive 9.93 Alive 15.49 Alive 11.65 Alive 17.48
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Snail No. 1 8 10 23 29 72 161 171 188 201 4 21 30 47 66 163 178 186 190 194 195 198 200 204 207 Initial Weight 7.42 8.36 8.32 9.24 7.78 7.72 8.31 6.69 7.63 6.7 8.72 10.63 8.42 8.42 9.29 10.51 7.21 8.38 8.82 10.31 9.28 8.22 9.5 9 8.03 Present Weight 4.77 2.63 3.46 3.23 4.54 2.44 2.33 2.35 2.62 2.04 7.73 8.24 6.18 6.67 7.41 8.83 5.26 5.42 6.49 9.14 7.83 5.28 6.83 6.5 5.7 Total Weight Loss 2.65 5.73 4.86 6.01 3.24 5.28 5.98 4.34 5.01 4.66 0.99 2.39 2.24 1.75 1.88 1.68 1.95 2.96 2.33 1.17 1.45 2.94 2.67 2.5 2.33 Status Weight Loss % Dead 35.71 Dead 68.54 Dead 58.41 Dead 65.04 Dead 41.65 Dead 68.39 Dead 71.96 Dead 64.87 Dead 65.66 Dead 69.55 Alive 11.35 Alive 22.48 Alive 26.60 Alive 20.78 Alive 20.24 Alive 15.98 Alive 27.05 Alive 35.32 Alive 26.42 Alive 11.35 Alive 15.63 Alive 35.77 Alive 28.11 Alive 27.78 Alive 29.02
72
Experiment 4. GAS Mortality of Different GAS Sexes in Plastic Tray with Soil Treatment Appendix 38. Final observation on the mortality of male GAS of various sizes (December 31, 2004).
S. No 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 % Mortality 20 mm Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 32 25 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 52 30 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 52 35 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 52 40 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive 68
73
Appendix 39. Final observation on the mortality of female GAS of various sizes. (December 31, 2004).
S. No 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 % Mortality 20 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 56 25 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 52 30 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 52 35 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive Alive 52 40 mm Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Dead Alive Alive Alive Alive Alive Alive Alive Alive 68
Appendix 40. Moisture observations in plastic trays throughout the whole observation period.
Moisture readings 10mm 15mm 20mm 25mm 30mm 35mm 40mm control1 control2 control3 Nov. 6, 2004 97.7 97.6 97.5 97.7 97.6 97.6 97.7 97.5 97.6 97.7 Nov.20,2004 96.7 96.9 96.5 96.5 96.6 96.3 96.6 97.1 96.7 96.8 Dec.4,2004 96.7 96.7 96.4 95.9 96.4 96 96.5 97.2 96.5 96.9 Dec.19,2004 96.8 96.9 96.5 95.8 96.5 96.6 96.8 97.3 96.7 97.1 Dec.31,2004 96.8 96.9 96.5 96.9 96.5 96.7 96.9 97.3 96.9 97.1
74
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Mortality of Golden Apple Snail

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Off-season Mortality of Golden Apple Snail, Pomacea canaliculata (Lamarck) and its Management Implications Abstract

Review of Related Literature

Plate 1. Sorting of GAS sizes and sex.

Plate 2. Drying the marked shell of the GAS.

Plate 3. Assigning numbers to GAS.

Plate 5. GAS sizes and sexes in netbags.

Plate 7. GAS in plastic tray with water and paddy soil.

Plate 8. The set -up of the plastic tray experiments.

Plate 9. Observations for GAS mortality.

Results and Discussion

Plate 10. GAS observations on mortality after one hour.

Experiment 1. GAS Mortality of Different GAS Sizes in Netbag Treatment

GAS Mortality (%)

60 50 40 30 20 10 0 10 15 20 25 30 35 40 GAS Size (mm)

2nd week 4th week 6th week 8th week

GAS Mortality (%)

80 plastic trays 60 40 20 0 10 15 20 25 30 35 40 GAS Size (mm) netbags

for each sex and size.

60 50 40 30 20 10 0 20 25 30 35 40 GAS Size (mm) Male Female

80 60 40 20 0 20 25 30 GAS Size (mm) 35 40 Male Female

Summary, Conclusions and Recommendation

management implicat ions were suggested.

Appendix 2. Weekly observation on the mortality of 10-mm GAS (December 4, 2004).

Appendix 6. Weekly observation on the mortality of 15-mm GAS (December 4, 2004).

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