Microplate Washer

BK-9622
User Manual

Biobase Biodustry(Shandong)Co., Ltd

 Preface
Thank you for purchasing BK-9622 Microplate Washer.

The Manual Using Statement

Please read this manual carefully before using the product. After reading, please place the
manual near the product so that it can be read when necessary. When the product location
changes, please attach the user manual.
Copyright notice: the copyright belongs to Biobase Biodustry(Shandong)Co., Ltd and it is not
allowed to reprint, plagiarize or modify this user manual without permission.

Quality Assurance
1. Biobase Biodustry(Shandong)Co., Ltd guarantees that the products manufactured by the
company have passed strict quality confirmation.
2. The maintenance cost will be charged according to the actual situation for the failure
caused by the user's own modification of the pipeline and function in the machine or the
product beyond the warranty period. For the failure and damage caused by force majeure,
the company does not provide free warranty service.

Safety Instructions
The safety precautions in the manual must be followed throughout the operation, maintenance
and repair of this product. Failure to follow these safety precautions will reduce the safety
protection of the equipment. The manufacturer declares that it is not responsible for the user's
violation of such requirements.

EC Declaration of Conformity
This medical device has been assigned to class I according to Annex VIII of the Regulation
(EU) 2017/745. It bears the mark

Whose single Authorized EU-Representative:

Name: Luxus Lebenswelt GmbH
Address: Kochstr.1, 47877, Willich, Germany
Email: [email protected]

Manufacturer:
Name: Biobase Biodustry(shandong) Co., Ltd
Address: No.9 Gangxing Road, High-Tech Zone, Jinan City, Shandong Province, China
Tel: +86-531-81219801/03
Email: [email protected]

1
 Catalogue
Preface ........................................................................................................................................ 1
Catalogue .................................................................................................................................... 2
Safety Symbol .............................................................................................................................3
Chapter 1 Overview .................................................................................................................5
1.1 Product Introduction ...................................................................................................... 5
1.1.1 Features ................................................................................................................ 5
1.1.2 Model Designation ...............................................................................................5
1.1.3 Electrical Safety Classification: ClassⅠ ................................................................5
1.1.4 Normal Operating Conditions ..............................................................................5
1.2 Basic Operating Principle .............................................................................................. 5
Chapter 2 Unpacking and Installation ..................................................................................... 6
2.1 Unpacking ......................................................................................................................6
2.2 Installation ..................................................................................................................... 7
2.3 Testing ............................................................................................................................7
Chapter 3 Equipment Operation .............................................................................................. 8
3.1 Interface Introduction .................................................................................................... 8
3.2 Function Settings ........................................................................................................... 8
3.2.1 Program Name ..................................................................................................... 8
3.2.2 Rinse .................................................................................................................... 9
3.2.3 Set up ................................................................................................................. 10
3.2.4 Introduction to Washing Board Function ...........................................................12
3.3 Alarm ........................................................................................................................... 12
3.3.1 The Wrong Washing Settings .............................................................................12
3.3.2 Liquid Shortage Alarm ...................................................................................... 12
3.4 Shutdown ..................................................................................................................... 13
Chapter 4 Considerations .......................................................................................................14
4.1 Precautions ...................................................................................................................14
4.2 Contraindications .........................................................................................................14
Chapter 5 Troubleshooting .................................................................................................... 15
5.1 No Response After Power on ...................................................................................... 15
5.2 Low Flow or No Flow of Washing Fluid .................................................................... 15
5.3 Washing Fluid Residue ................................................................................................15
5.4 Washing Liquid Overflow ........................................................................................... 15
5.5 Washing Head Keep Dispensation When Washing .....................................................16
5.6 The Positive Pressure Interface Spurt Water ............................................................... 16
5.7 The Place of Washing Head and the Plate Well Mismatching ....................................16
Chapter6 Repair and Maintenance .........................................................................................17
6.1 Maintenance .................................................................................................................17
6.2 Transportation and Storage ..........................................................................................17

2
 Safety Symbol
1. Definition of electrical symbols, safety signs and warning signs
Symbol Description
For internal and external grounding. Please ensure that the
instrument is well grounded.
Power-on
Power-off
Caution: To avoid injury, death, or damage to the
instrument, the operator must comply with the instructions
in the manual.
CE mark: This medical device has been assigned to class I
according to Annex VIII of the Regulation (EU) 2017/745.
It bears this mark
European Authorized Representative: Represents the
authorized representat- ive of the European Union.

Indicates correct upright position of the transport package.

Contents of the transport package are fragile therefore it

shall be handled with care.

Transport package shall be kept away from rain.

Transport package shall not be rolled.

2.Security Abstract
Pay attention to the following general safety precautions during the operation or maintenance
of this instrument. If the customer fails to comply with these precautions or any clear warning
in this manual, we will not be responsible for any compensation for personal injury or
machine damage.

Be sure to read this manual carefully before use and please keep it
safe.

Do not use the product for situations other than those described in the
manual.
This product is only used in the case described in the product
manual.

Before connecting the power supply, please check that the power
supply meets the rated input value of the instrument, and make sure
that the switch is in the off state.

3
 Protective grounding: Before connecting power, make sure the
connection protection grounding in order to prevent electric shock.

Necessity of protective grounding: Do not cut inside or outside

protective grounding wire or interrupt the connection of the
protective earth terminal. This will cause a potential shock hazard,
which may bring harm to the human body
Fuse: Only use the rated current, voltage, and specific form of Fuse
(normal fuses, time delay, etc). Do not use different specifications
fuse or circuit fuse holder, otherwise it may cause electric shock or
fire hazard.
Please do not tear down the shell of the instrument: the operator
should not tear down the shell of the instrument. Parts replacement
and internal adjustment can be performed by qualified maintenance
personnel only.

Do not operate under the air condition of explosive or corrosive.

Especially in the flammable gas or corrosion environment

Turn off the power switch and all connections when change its
location.Products should be placed in the appropriate location,
where can be quickly disconnect power supply connection when in
emergency situations.Be careful when handling, especially the high
bottle.Please attach the manual with the products when resetting
position.
Check and ensure that the settings of AC input voltage are consistent
with the fuse specification. There is no abnormality on the surface
of power cord. Make sure to cut off the power supply cord or shut
off the power switch before inspection.
If there is any abnormal phenomenon or fault, please stop using
immediately and cut off the power cord or disconnect the power from
the power supply distribution chest and you do not use before
product maintenance well.
Please do not dismantle or change the product. If it must be changed,
please contact Biobase Biodustry(Shandong)Co., Ltd.

Do not let water or metal objects enter into the product inside.

It won’t in the product warranty range, if the product damage is

caused by using the wrong power grid to enter.

The end of the cleaning head needle is very sharp and may cause
physical injury. Do not place hands or fingers under the cleaning
head needle when the instrument is running.

4
 Chapter 1 Overview
1.1 Product Introduction
Microplate Washer is a newly developed model produced by our company, which applying
modern machinery and electronic technology to the field of medical equipment and is in
consideration of our country's medical department ELISA detection status. This machine is
controlled by microcomputer and the operation is quite easy and quick. Plate washing solution
can be arbitrarily set. The single row to the whole row washing can be automatic and accurate
completed. The machine will play a major role in the medical tests in consideration of its
exquisite structure, reliable quality and perfect after-sales service.
1.1.1 Features
(1) Suitable for various specifications of ELISA Plate:96 is available.
(2) The position of the cleaning head rinse can be adjusted according to the user’s needs.
(3) Parameter can be selected in order to complete different cleaning effect.
(4) Adopt 7-inch color touch screen, easy to operate and use.
1.1.2 Model Designation
The rule of the model designation is as follows:
BK-XX X X
Design sequence
ELISA plate placement mode: 2
The number of ELISA plate hole: 96 and below 96
Brand
Example: BK-9622 type can wash 96 and below 96 microplate and has 2 kinds of placing way,
and the design sequence is 2.
1.1.3 Electrical Safety Classification: ClassⅠ
1.1.4 Normal Operating Conditions
(1) Ambient Condition
a. Ambient temperature: 5℃～40℃.
b. Relative humidity: ≤80﹪RH.
c. Working pressure: 76.0KPa～106.0KPa.
d. Working surrounding: Without the interference of strong magnetic field and
mechanical vibration, no corrosive gas.
e. Elevation requirements: Below 2000m.
(2) Power supply: Single-phase AC power supply with grounding protection. Voltage:
AC220V/110V. Frequency: 50Hz/60Hz.
1.2 Basic Operating Principle
Microplate Washer drives cleaning fluid into the microplate and waste exhaust with the
principle of positive and negative pressure. The cushioning bottle is pressured during washing.
Therefore, don't open the bottle or disconnect the pipe during pump starting.
When the touch screen gets the signal to start cleaning, MCU sends the pump signal and send
pulses to the stepper motor drive circuit. Stepper motor forth moving, open the valve, cleaning
MPP and then washing plates according to the selected plate washing solution. Man-machine
conversation is realized through liquid crystal display.

5
 Chapter 2 Unpacking and Installation
2.1 Unpacking
The machine has a special packing. After open the packing cases, user should carefully
check whether the instruments and accessories are in accordance with list of items in Table
2-1.
If you find any missing or damaged, please immediately contact the local dealer or
manufacturer and it will be helpful for processing.
Note: Please keep packaging materials for future transit or return to factory
maintenance.

Table 2-1 Materials List

No. Name Units Qty. Note

1 Microplate Washer PCS 1

2 Certificate card PCS 1

3 Power Line PCS 1

4 Resistance pen PCS 1

5 User Manual PCS 1

6 250V/3A Fuse PCS 2

7 Washer cleaner PCS 1

12 needles washing
8 PCS 1 12 needles
head
8 needles washing
9 PCS 1 8 needles
head
10 Wash Bottle PCS 2

11 Waste Bottle PCS 1

12 Cushioning Bottle PCS 1

13 Pipe Flushing Bottle PCS 1

14 Silicone tube Set 1

15 Inspection report PCS 1

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2.2 Installation
As shown in figure 2-1, Microplate Washer’s water system consists of washer, wash bottle,
waste bottle, pipe flushing bottle, electromagnetic valve, washing head and pipeline.
Reference to the water system schematic diagram connected the wash bottle, waste bottle,
cushioning bottle,pipe flushing bottle according to the interface name and complete to connect.
The washing head has two interfaces, the upper interface is the liquid inlet which connected
with the silica gel tube with line label "up", the lower interface is the waste liquid outlet which
connected with the silica gel tube with line label "down".
Note: Because of the positive, negative pressure principle is used, each time the user
open the bottle caps, and please tighten the caps of each drum.

Figure 2-1 Schematic diagram of hydraulic system

Note: Do not use hand to push and pull the washing head fixing frame, it can
automatically reset and initialization.

2.3 Testing
After all above works are completed, put some distilled water or pure water in the wash bottle,
no more than a full bottle of 4/5 and tighten the caps. Insert the power cord into power supply
input pin on the rear panel, then plug the power line to power supply AC220V/110V,
50Hz/60Hz. Notice of low or high voltage power grid volatility and places, users should
increase UPS or AC voltage stabilizer before use.

7
 Chapter 3 Equipment Operation
3.1 Interface Introduction

Figure 3-1
Program Name: Define name according to different washing methods of different projects,
and set common washing methods by yourself.
Needle Number: 8 rows of needle washing head and 12 rows of needle washing head can be
selected freely.
Volume: 50ul-950ul, adjustable, and the interval of filling volume is 50ul.
Asp. Time: 0.1s-99.9s adjustable, Asp. time interval is 0.1s.
Wash Times: 1-999 times adjustable.
Soak time: (Shake time): 0-999s adjustable, soak time interval is 1s.
Clean Mode: "Sock" and "Shake" can be selected. When the cleaning method is "shake", the
soak time is the shake time.
Plate type: You can choose "flat ", "U-shape" and "V-shape" to adapt to different types of
microplate.
Cleaning Fluid: Choose "A", " B" and " water". (the washing machine is equipped with five
bottles in total, and the rest are Waste bottle and Cushioning bottle).
Start column: The start position of the board washing function can be selected. The default
options are 1-12 (8 rows of needle washing head) and 1-8 (12 rows of needle
washing head).
End column: The end position of the board washing function can be selected. The default
options are 1-12 (8 rows of needle washing head) and 1-8 (12 rows of needle
washing head).
3.2 Function Settings
3.2.1 Program Name
1.Click "New Project" to enter the following interface, enter the program name, click "Enter"
to save the name and enter the program editing interface as shown in Figure 3-2.

Figure 3-2

8
2. The program parameters can be adjusted according to the actual washing needs. (see 3.1
for details).

Figure 3-3
3. Click "Save" to save the new program. Wait a moment to save successfully. The saving
interface is as follows.

Figure 3-4 Figure 3-5

Note:
After saving, the interface will return to the main interface, as shown in Figure 3-1.
Once a program is created, it cannot be deleted, but the program name and parameters
can be modified according to the following operations:
 Choose the program in the main interface, click the program name to modify the
name and click "Enter" to finish the modification.
 Click any parameter to modify, after that, click "Start" to run the modified
program, after the program runs, it can be automatically saved.

3.2.2 Rinse

1. Click the "Rinse" button to enter the following interface.

Figure 3-6
2. The flushing time of the pipeline can be adjusted by up and down arrows, and the

9
 adjustment range is 0-999S, which can be adjusted freely according to different needs.
(long press can realize rapid change of time).
3. After setting the pipeline flushing time, click "Rinse". Then enter the pipeline flushing state.
After the preset time, the washer returns to the initial interface.

3.2.3 Set up
1. Click the "Setup" button to enter the following interface.

Figure 3-7
2. Click the "Check" button to enter the following interface.

Figure 3-8

: Indicates that the bottle is normal.

: Indicates that the bottle is abnormal.

3. Click "Return" to return to the previous interface.
4. Click the " Adjust" button to enter the following interface.

Figure 3-9
 Center 1, that is, the position of the aspirating needle (long needle) when it is in the
center of the first row of holes in the microplate. Adjust or to move the
horizontal position of the washing head. (The direction of adjustment shall be
horizontal, with the suction needle facing the center of the hole.)
 Two points is the same as above. The first point is the position where the aspirating

10
 needle sticks to the bottom of the left wall cup of the hole, and the second point is the
position where the aspirating needle sticks to the bottom of the right wall cup of the
hole. (The debugging direction is horizontal.)
 Overflow, the operation mode is the same as above, and the standard position is that
the tip of the extraction needle is located in the center of the hole mouth. (Debugging
direction is vertical.)
 Bottom in the same way as above. The standard position is that the tip of the
aspirating needle is at the bottom of the hole. (Debugging direction is vertical.)
 Center 12, that is, the position of the aspirating needle (long needle) when it is in the
center of the last row of holes in the microplate. The operation mode is the same as
above.
5. After the position is adjusted, click "Save" to save the debugging result. (Restore the
position before saving when resetting, and cannot reset after saving.)
6. Click "Version" to enter the following interface.

Figure 3-10
7. Click the "Language" button to enter the following interface.

Figure 3-11
8. Click the drop-down box after selection to pop up a menu of two languages. After selecting
English, all the interfaces are in English (The same as in Chinese.) except that this interface
is always bilingual. The default language of the first boot after the first burn is Chinese.
9. The "Aging" function is exclusive to the debugging staff, and users do not need to use it.

Figure 3-12 Figure 3-13

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10. Rinse (automatic rinse interval) which can be set to 0-24, is based on a complete plate
washing project. Automatic pipeline flushing will not be carried out until the number of
intervals has been set. For example, the automatic flushing interval is set as 2, then the
washing machine will automatically flush the pipeline after completing the plate washing
task for 3 times.
3.2.4 Introduction to Washing Board Function
1. After all parameters are set, click "Start " to enter the washing interface.

Figure 3-14 Figure 3-15

2. During the washing process, the number of “Wash times No.” and “Times Remina”
number of washing can be displayed. The operation keys are "Pause" and "Stop".
After clicking the "Pause" key, the current plate washing can be stopped after the end of the
action, "Pause" key is replaced by "Go", and then click "Go" again, the plate washing
machine will resume normal operation.
After clicking the "Stop" key, the current plate washing operation will immediately end and
return to the original position.
(Note: During the pause, if the washing machine is in the state of soak / shake , the
timing method is different. If the "Soak" mode is selected, the soaking time starts
from the washing plate. If the "Shake" mode is selected, the vibrating plate time
starts from the vibrating plate. If paused in soak mode, the timing is suspended.)
3.3 Alarm
3.3.1 The Wrong Washing Settings

Figure 3-16
When the start column and end column of the washing board are inconsistent (that is, the
number of the start column is greater than that of the end column), the above alarm will be
generated. Click back and readjust the number of washing columns.

3.3.2 Liquid Shortage Alarm

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 Figure 3-17
Take the clear water shortage alarm as an example. When the surplus of the Pipe flushing
bottle is insufficient, the above alarm will occur. Click "Close " to end the alarm tone. During
this operation, the bucket will not be given a liquid shortage alarm. Click "Cancel", that is, the
washing machine will end this operation. After the washing machine returns to its original
position, replace the bucket and tighten the bucket cover after adding. Make sure that there is
no mistake before the last plate washing project. Click "Close" or "Cancel" when judging the
alarm according to the actual liquid margin in the bottle.
(Note: It is forbidden to operate the washing machine when the bottle is empty and the
end is not tightened!)
When several bottles are short of liquid at the same time, the alarm message will give multiple
alarm messages according to the actual situation in the bottle. For example:

Figure 3-18
3.4 Shutdown
When the microplate washer is finished running, please do not turn off the power supply
directly. Click the "Shutdown" key on the main interface, then the washing machine will be
initialized and reset. After the reset, enter the following interface. At this time, you can choose
to turn off the power supply directly or click the "Return" key. Click "Return" to return to the
initial interface and cancel the shutdown.

Figure 3-19

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 Chapter 4 Considerations
4.1 Precautions
1.The user should read this specification carefully before using the instrument and master the
correct method.
2. When the liquid aspirating needle or liquid injection needle is blocked, use the special
needle for dredging.
3. When a row of microplate is not the whole strip, it is necessary to supplement the plate to
ensure that the microplate can be operated again under the whole strip state, so as to
prevent the leakage of liquid and corrosion of machine parts.
4. Do not allow liquid to enter the pump. Dispose of the waste liquid in the waste liquid bottle
in time after each use. Before each start-up, check whether the waste liquid bottle is empty.
During the use, the waste liquid shall not exceed 80% of the total volume of the waste
liquid bottle. Users should pay more attention to this point when cleaning more than two
enzyme plates every day.
5. Avoid using in high temperature, high humidity and dusty environment.
6. Shut down immediately after use.
7. Before replacing the fuse tube, unplug the power plug, screw off the fuse tube cover, take
out the fuse tube, and connect the standard fuse tube with the machine (Φ5× 20mm, 3A).
Put it into the fuse box and tighten the fuse cover.
Note: do not use the fuse with unknown parameters at will, otherwise it may cause fire
and electric shock risks.
4.2 Contraindications
Contraindications: None.

14
 Chapter 5 Troubleshooting
5.1 No Response After Power on
1. No voltage supply or the power socket might be loose. Check the power socket whether
there is a voltage .Whether the connection of the plug and socket is secure .Put the power
plug into the socket again.
2. The power fuse fusing. Beside the power socket which is at the back of the instrument has
two fuse holders, unscrew the fuse with the hand, check whether the fuse fusing. If the fuse
burn-out, change the fuse in same model and specification.

NOTICE: When replacing the fuse, making sure to unplug the power plug from the
instrument, in order to avoid the risk of electric shock risk of electric shock.

5.2 Low Flow or No Flow of Washing Fluid

1. Check whether a sufficient amount washing liquid in the washing bottle.
2. Check the dispensation volume parameter is set too small
3. The cover of the washing bottle is not tighten, the screw from the cover is loose, there is air
leakage between cap and screw.
4. There is beam or gather in the silicone tube, the tube blocked. Check the silicone tube and
make sure there is no beam or gather. If is beam or gather on the tube, make sure straighten
out the gather and cut off cracked parts or replace parts of the silicone tube.
5. The injection position is too low, which causes the long needle (aspirating needle) to suck
the injected detergent away. When washing the plate, the position of the long needle of the
cleaning head should be about 1mm below the hole port of the enzyme label plate, and it is
not allowed to penetrate too much below the hole mouth. if it is necessary to fill the
microplate, the long needle can be adjusted to a position slightly higher than the hole
bottom by about 0.5mm. (See 3.2.2 setting standard of anti overflow height for details.)
5.3 Washing Fluid Residue
1. The cover of wash and waste bottle is loose, or the screw is loose, there is air leakage
between cap and screw.
2. The aspiration needle is blocked. Check the aspiration needle whether there is bulkhead,
Use the nozzle cleaner to clean up the tube ,and go on the operation of washing tube.
Washing some times and make sure the waste is aspirated in the waste bottle.
3. The aspiration system is blocked .Check the aspiration system whether there is blocked.
Removing the silicone tube if it is blocked .Clean up the inside of silicone tube, make sure
the tube is good.
4. The aspiration position is too high, the aspiration needle didn’t reach the bottom of
microplate well. Check and make sure the distance of aspiration needle and microplate well
is not too big .If the aspiration did not reach the bottom of microplate, please adjust the
touch the bottom position parameter.(See 3.2.2 bottom touch height setting standard for
details.)
5.4 Washing Liquid Overflow
1. Check and make sure the dispensation volume parameter is not too big.
2. The speed regulator valve is not good, the speed of dispensation is too fast.
Counterclockwise rotating speed control valve, while adjusting the speed control valve

15
while watching the dispensation speed until the dispensation speed is normal.
3. The distance between the long needle of the cleaning head and the end face of the
microplate cup of the enzyme plate is too large, which causes the overflow function not to
play a role. Adjust the "anti overflow" parameter, adjust the end of the long needle of the
cleaning head to about 1 mm below the end face of the hole of the microplate. (See 3.2.2
setting standard of anti overflow height for details)
5.5 Washing Head Keep Dispensation When Washing
1. The valve is not working. Please connect with the manufacturer.
2. Main control board is not working, please connect with the manufacturer.
5.6 The Positive Pressure Interface Spurt Water
1. The waste bottle is full and the cushioning bottle is not installed as required,the waste liquid
was aspirated into the vacuum pump and discharge along the positive tube .Turn off the
machine and stop the working ,and then pour out the waste liquid of the waste bottle and
cushioning bottle .Connect the waste bottle and cushioning bottle (The wash bottle may be
not connected at this time.) before open the machine. The instrument idle running for
minutes so that the waste liquid in the vacuum pump may drain out.
2. The positive and negative pressure pipeline connection is wrong so that the waste liquid
may be absorbed into the pump directly. According to the operating manual, check the
connection of the pipeline is correct.
The waste liquid should not immersed in the vacuum pump for a long time.
Or the component of pump may corroded by waste liquid and damage the
vacuum pump.

5.7 The Place of Washing Head and the Plate Well Mismatching
1. The ELISA plate not be put into the groove of the tray. Reset the ELISA plate again. Make
sure the bottom of ELISA plate and the tray is contacted entirely and be flat.
2. The position calibration is not set properly, please readjust the parameter value. (See 3.2.2
for details.)

16
 Chapter6 Repair and Maintenance
6.1 Maintenance
 Use the instrument correctly and maintenance is useful for the machine’s properly
functioning and extend the operating life of the equipment .
 Turn off the power before the maintenance .
1. The storage environment of the instrument shall be dry to prevent moisture, corrosion and
strong electromagnetic interference sources.
2. When replacing the fuse tube in the fuse, first cut off the power supply and replace it
according to the specification of the fuse tube.
3. The instrument has been precisely adjusted before leaving the factory. In case of abnormal
or abnormal operation of the instrument, contact the manufacturer in time, and the user is
not allowed to disassemble and adjust it at will.
6.2 Transportation and Storage
1. The storage environment of the instrument should be kept dry. Prevent the damp and
corrosion and keep far away from the interference source of strong electromagnetic fields.
2. The power should be turn off when change the fuse. And change the fuse according to the
specification as marked.
3. The instrument is adjusted precisely when it is ex-factory. Please connect with the
manufacturer when the machine is abnormal or not working properly. The user should not
dismantle and adjust the machine.

17
Biobase Biodustry(shandong) Co., Ltd
Tel: +86-531-81219801/03
Email: [email protected]
Address: No.9 Gangxing Road, High-Tech Zone,
Jinan City, Shandong Province, China