Protein Notes (Dipesh Routh)

Dipesh Routh
Biochemistry and Clinical Pathology
PROTEINS
1. Definition, classification of proteins based on composition and

solubility with examples
2. Definition, classification of amino acids based on chemical
nature and nutritional requirements with examples
3. Structure of proteins (four levels of organization of protein
structure)
4. Qualitative tests and biological role of proteins and amino
acids
5. Diseases related to malnutrition of proteins
Definition.
➢ Proteins are naturally occurring polymers made up of amino acids.
➢ Almost everything that occurs in the cells involves one or more Proteins.
➢ Proteins provide structure, cellular reaction and carried out the tasks.
➢ 20 amino acids are found in protein and they are called standard amino
acid. These amino acids contain the carboxyl group and the amino group
attached to α carbon.
Role of protein.
➢ Protein performs difference role in the living system.
➢ Proteins which catalyse by your chemical reactions are called enzymes.
➢ Proteins are responsible for transportation of metabolites fructose, Glucose
or Gases (like Oxygen, Carbon dioxide) are called transport proteins.
➢ Protein which are responsible for to protect from infection and other toxic
➢ substances are called antibiotics or defense proteins.

➢ Proteins which are required to give strength to cells or tissue are called
structural proteins.
➢ Proteins which are required to carry out mechanical work are called muscle
proteins.
Classification of proteins based on composition.
1. Complete Proteins: – Proteins which contain all the essential amino

acids in required quantity are called complete Proteins.
2. Incomplete Proteins: - Proteins not containing all the essential amino

acid are called incomplete protein.
Also classified as –
1. Simple Proteins
2. Conjugated Proteins
3. Derived Proteins
1. Simple Proteins: - Simple protein contain only amino acid reduce and
other intimately bound material. Ex- Albumins, globulins, glutelin etc.
2. Conjugated Proteins: – Conjugated proteins contain in addition to

polypeptide chain others substance or groups which impart characteristics
properties. Ex- Nucleoproteins, Glycoproteins, Haemoglobin,
Phosphoproteins etc.
3. Derived Proteins: - Derived protein are derived from partial to complete

hydrolysis from the simple or conjugated proteins by the action of acids, alkalis
or enzymes. Ex- Peptones, peptides, proteoses etc.
Classification of protein based on solubility.
It is divided into two types-
a. Globular protein - It is made by the irregular amino acid sequence and

forms the rounded or spherical structure.
1. It is generally soluble in water.
2. It is also called as functional protein and it is more sensitive towards PH
and temperature change.
b. Fibrous protein - It is made by same type of amino acid sequence and forms
the long narrow structure.
1. It is generally Insoluble in water.
2. It is also called as structural protein and it is less sensitive towards PH
and temperature change.
AMINO ACIDS
Definition
1. Amino acids are building blocks of protein.

2. Amino acids are compound that contain and amino group and carboxyl
group and the amino group attached to alpha carbon.
3. The key elements of an amino acid are Carbon (C) Hydrogen (H)
Oxygen (O) and nitrogen (N).
4. There are there are 20 amino acids at present in our body in which 9
Essential and 2 Non-essential amino acids.
Properties of Amino Acids
A. Physical properties of the Amino acids.

1. Solubility.
• All amino acids are Soluble in water but their Solubility various to a
great extent.
• Solubility depends on the nature of R- group. i.e., polarity of the amino
acid.
• Polar amino acids are highly Soluble in water.
• Non-polar amino acids are highly Soluble in organic solvents like
chloroform, ether etc.
2. Acid and base behaviours.
• Amino acids contain the acidic carboxyl group (-COOH) and the basis
group amino (-NH2) Hence amino acids are called as amphoteric
molecules or ampholytes (i.e., Amphoteric electrolytes).
3. Optical activity.
• All standard amino acid except glycine has an asymmetric carbon atom
due to these amino acids are optically active.
B. Chemical properties of the Amino acids.
1. Ninhydrin reaction:
Amino acid + Ninhydrin Purple/Blue Pigment + Aldehyde
2. Reaction with Dansyl Chloride:

Amino acid + Dansyl Chloride Fluorescent Dansyl derivative
Of amino acids.
3. Reaction due to COOH Group: -

➢ Salt formation with alkalis.
➢ Ester formation with alcohols.
➢ Amide formation with amines and decarboxylation
.
Classification of amino acids based on chemical nature.
On the basis of chemical nature amino acids are divided as-
a. Acidic amino acid- Ex- Aspartic acid, Glutamic acid.
b. Basic amino acid- Ex- Histidine, Lysine, Arginine.
c. Hydrophilic amino acid- Ex- Asparagine, Glutamine.
d. Hydrophobic amino acid-Ex- Leucine, isoleucine, valine.
e. Sulphur containing amino acid- Ex- cystine, Methionine.
f. Aromatic amino acid- Ex- Phenylalanine, Tyrosine.
Classification of amino acids based on nutritional requirements
1. Essential Amino Acids.

• The amino acids which cannot be synthesized in the body but are
required for normal function of body are called as essential amino acids.
• These amino acids should be supplied through diet.
2. Non-Essential Amino Acids.
• The amino acids which are synthesized in the body are called as non-
essential amino acids.
S. N Essential Amino Non-Essential
acids Amino acids
1. Valine Alanine
2. Histidine Asparagine
3. Leucine Aspartic acid
4. Phenylalanine Cysteine
5. Tryptophan Glutamic acid
6. Lysine Glutamine
7. Alginine Glycine
8. Methionine Cysteine
9. Threonine Proline
10. Isoleucine Serine
Structure of proteins (four levels of organization of protein structure).
Due to arrangement of the amino acids in the protein, we find the four level of
organisation/modification in the proteins.
1. Primary protein: - It is the simple/basic/polypeptide chain like

proteineous structure formed by the joining of amino acid by the help of
covalent or peptide bond. Its biological activity mainly depends on the,
types of amino acids. Many of genetic disorders are occurs due to
changes in the primary protein structure.
2. Secondary protein: - Secondary structure forms by the interaction

between the polypeptide chain of primary protein amino group and
carboxyl group. It mainly presents two structural forms-
• α secondary protein (Helix Form).
• β secondary protein (Sheet/Plate form).
3. Tertiary protein: - Tertiary protein form by the further chemical

modification between the secondary protein. It is stabilized due to
presence of the H-bond, electrostatic forces, disulphides bonds, and
Vander walls forces.
4. Quaternary protein: - The quaternary structure of a protein is the

association of several protein chains or subunits into a closely packed
arrangement.
Qualitative tests for Proteins and Amino acids.

1. Heat test :
• When protein solution is heated in boiling water both the protein gets
coagulated and lose their biological activity.
• This is called thermal denaturation of proteins.
• e.g. – Boiling the eggs.
2. Test with trichloroacetic acid (TCA) :

• TCA is normally used to precipitate proteins from their solution. TCA
denatures the proteins.
3. Biuret Test :
• Biuret reagents consists of copper sulphate in an alkaline medium when
proteins are treated with Biuret reagent it’s shows a violet colour.
4. Hydrolysis Test :
• Proteins on hydrolysis gives free amino acids Hydrolysis can be carried
out by acids like HCL, H2SO4, etc. Or Alkalis like – NaOH, KOH etc.
5. Xanthoproteic test :
• Nitration of aromatic amino acids of protein give yellow colour
concentrated nitric acid is used for Nitration.
6. Millon’s Test :
• Phenolic group of tyrosine of proteins react with mercuric sulphate in the
presence of sodium nitrate and sulphuric acid to give red colour.
7. Precipitation test :
• Protein are Precipitated by using different agents the common
precipitation agents are salt, Organic solvent heavy metal ion, acids etc.
• Salt – Ammonium sulphate, Sodium chloride.
• Acids – Trichloroacetic acid (TCA), Acetic acid, Hydrochloric acid.
• Organic solvents – Acetone alcohol
• Heavy metal ions – Ammonium molybdate, Copper or Mercury salts.
Biological role of proteins and amino acids.
• Proteins give amino acids on hydrolysis during digestion and amino acids
are the building blocks required for a cell to synthesis for proteins.
• Proteins are the structural component of protoplasm cell and tissues.
• Enzymes and few hormones are Proteins in nature antibiotics,
haemoglobin are also Proteins.
• Protein is one of the important components of diet it is required to
maintain growth and healthy functioning of the body.
• In the cell, cell membrane is also made up by the protein, protein play the
role in the transporting the cellular and outer material through the active
or passive transport.
• In our body some amount of protein stored, for the starvation, critical
condition for energy.
• During the clinical condition protein also play the major role (protein
excrete in urine, during blood examination)
Diseases related to malnutrition of proteins.
A. Kwashiorkor :
• The symptoms of the diseases slow down the growth, oedema and
change in skin, hair pigmentation and texture.
• Frequently there is liver enlightenment there is vomiting and diarrhoea
and stools contain much undigested food.
• The course of this disease due to large family size, poor mental health,
poor environmental conditions and delayed supplementary feeding.
Note: - This disease appears most commonly in children between the

ages of 1 to 4 year.
B. Nutritional oedema :
• It results from long contained loss of protein and usually occurs in
famine areas. The Proteins deficiency in adults is very rare.
• The deficiency symptoms include loss of weight reduced fat,
infections, frequent loose stools delay in healing of wounds and Oedema.
• Use of soybean, milk and eggs and other nutritious diet can cure the
Protein deficiency syndrome in adults.
C. Marasmus :
• It is a disease of infants below one year of age.
• Its cause is Proteins and carbohydrate or other nutritional factor
deficiencies.

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Dipesh Routh

Biochemistry and Clinical Pathology

1. Definition, classification of proteins based on composition and

➢ substances are called antibiotics or defense proteins.

Classification of proteins based on composition.

1. Complete Proteins: – Proteins which contain all the essential amino

2. Incomplete Proteins: - Proteins not containing all the essential amino

2. Conjugated Proteins: – Conjugated proteins contain in addition to

3. Derived Proteins: - Derived protein are derived from partial to complete

Classification of protein based on solubility.

It is divided into two types-

a. Globular protein - It is made by the irregular amino acid sequence and

1. Amino acids are building blocks of protein.

Properties of Amino Acids

A. Physical properties of the Amino acids.

2. Reaction with Dansyl Chloride:

3. Reaction due to COOH Group: -

Classification of amino acids based on nutritional requirements

1. Essential Amino Acids.

Structure of proteins (four levels of organization of protein structure).

1. Primary protein: - It is the simple/basic/polypeptide chain like

2. Secondary protein: - Secondary structure forms by the interaction

3. Tertiary protein: - Tertiary protein form by the further chemical

4. Quaternary protein: - The quaternary structure of a protein is the

Qualitative tests for Proteins and Amino acids.

2. Test with trichloroacetic acid (TCA) :

Biological role of proteins and amino acids.

Diseases related to malnutrition of proteins.

Note: - This disease appears most commonly in children between the

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